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Journal articles on the topic 'Callusing Response'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Singh, Neerja. "Effect of Indole Butyric Acid (IBA) Concentration on Sprouting, Rooting and Callusing Potential in Bougainvillea Stem Cuttings." Journal of Horticultural Sciences 7, no. 2 (2012): 209–10. http://dx.doi.org/10.24154/jhs.v7i2.378.

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A study was conducted in 2008-09 to investigate the influence of Indole Butyric Acid (IBA) at 0, 1000, 1500 and 2000ppm concentrations on rooting potential in hardwood cuttings of four varieties of bougainvillea, i.e., Louise Wathen, Thimma, Mrs. Butt and Shubhra. The experiment was laid out in Randomized Block Design with sixteen treatments and four replications. Results indicated that both IBA concentration and variety had significant effect on sprouting, rooting, callusing and establishment of cuttings. Louise Wathen cuttings treated with 1000ppm IBA were superior in response with 85.39% sp

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2

Rowe, C. A., D. P. Logan, and P. G. Connolly. "Plant callus response to egglaying by chorus cicada and its effect on egg hatch." New Zealand Plant Protection 69 (January 8, 2016): 305–9. http://dx.doi.org/10.30843/nzpp.2016.69.5901.

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Chorus cicada Amphipsalta zelandica (Biosduval) is an endemic species of cicada that is a pest of kiwifruit Adult female cicadas cause damage to kiwifruit canes when egg laying The oviposition wound often stimulates the local formation of callus tissue in kiwifruit canes The effect of eggnest callusing on the proportion of eggs to hatch successfully was investigated as a potential plant resistance trait for selection The proportion of callus tissue covering the eggnest and the number of eggs hatched were measured from eggnests in canes of two kiwifruit cultivars Hayward and Zesy002 and stems o

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3

S C Fernando. "Use of Arabinogalactan Proteins in Coconut (Cocos Nucifera L.) Tissue Culture: An Alternative Approach for Improved Tissue Response." CORD 27, no. 2 (2011): 8. http://dx.doi.org/10.37833/cord.v27i2.112.

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Arabinogalactan proteins (AGPs) are extra-cellular proteins involved in plant growth and development. The effects of these proteins on in vitro response of different species are well documented. This study assessed for the first time the role of AGPs on tissue culture of coconut, a highly recalcitrant species in vitro. Coconut (Sri Lanka Tall) plumules were cultured in medium containing 2, 4-dichlorophenoxyaceticacid (2,4-D) for callogenesis. Calli were multiplied by subculturing onto freshly prepared callusing medium. Somatic embryos were induced by transferring of calli to medium with 2/3 co

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Chaudhuri, Pinaki, Rupak K. Sengupta, and Partha D. Ghosh. "An investigation of gamma-radiation sensitivity on in vitro study of Hordeum vulgare L." Acta Societatis Botanicorum Poloniae 64, no. 2 (2014): 163–64. http://dx.doi.org/10.5586/asbp.1995.022.

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Gamma-radiation sensitivity was studied on <i>in vitro</i> condition in <i>Hordeum vulgare</i> L. The variation of callusing response assessed with the increasing level of gamma-radiation treatment and regeneration delayed at higher dose level.

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5

Singh, Sanjay. "Physio-biochemical Changes during Adventitious Root Formation in Gmelina arborea Roxb. Bud Sprout Cuttings." Plant Science Archives 9, no. 3 (2024): 23–30. http://dx.doi.org/10.51470/psa.2024.9.3.23.

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Changes in the endogenous moisture content and biochemical viz., soluble sugar), phenols, o-phenol and peroxidase activity at different stages of adventitious rooting in auxin-treated (5 mM IBA) and non-treated miniature bud sprout cuttings of Gmelina arborea Roxb. were investigated at 0 h, 24 h, 3 days, 7 days, 2 weeks and 3 weeks of planting to elucidate the process of adventitious root formation at physio-biochemical level. Simultaneously adventitious rhizogenesis parameters viz., proportion of live green sprouts (%), callusing (%) and rooting (%) were recorded. Two weeks after planting, th

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MAHINDRAKAR, AAKANKSHA, RAJIV KUMAR, C. ASWATH, and P. M. MUNIKRISHNAPPA. "Callusing and regeneration response of in vitro derived leaf explants of gerbera (Gerbera jamesonii)." Indian Journal of Agricultural Sciences 88, no. 6 (2018): 860–64. http://dx.doi.org/10.56093/ijas.v88i6.80584.

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Gerbera (Gerbera jamesonii Bolus ex. Hookes F.) is one of the most popular commercial flowers worldwide used both as cut flower and potted plant. Conventionally, it is propagated through vegetative means such as divisions of clump which is very slow process and not commercially viable. Rapid multiplication of elite hybrids/genotypes with improved quality traits, has been achieved by using both direct and indirect tissue culture methods. Gerbera hybrid seeds obtained by half-sib mating were raised in half strength MS medium. The earliest days for seed germination(2.00), first leaf development (

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7

Tripathy, S. K. "High throughput anther culture response in an upland rice cross ‘Khandagiri x Dular’." Journal of Environmental Biology 43, no. 03 (2022): 420–29. http://dx.doi.org/10.22438/jeb/43/3/mrn-2020.

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Aim: The experiment was conducted to maximize anther culture response in an upland indica rice cross amenable for drought tolerance breeding. Methodology: The cold pre-treated anthers of a cross Khandagiri (drought sensitive) x Dular (drought tolerant) were cultured on N6, SK3, MS and CIM media at varying concentration of hormones (2,4-D, Kn and NAA) to assess callusing response. Embryogenic calli were placed on regeneration medium (RM) with varying concentration of Kn, BAP and NAA for plant regeneration. The plantlets were acclimatized in half-strength MS basal liquid medium for one week befo

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Rani, Archana, M. Kumar, and Sanjeev Kumar. "Effect of phytohormones on shoot apex and leaf explants of Withania somnifera (Ashwagandha)." Journal of Applied and Natural Science 8, no. 1 (2016): 412–15. http://dx.doi.org/10.31018/jans.v8i1.808.

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An efficient protocol for callus induction of Withania somnifera through in vitro culture of shoot apex and leaf explant was standardized. Of the various combinations of phytohormones evaluated, MS media supplemented with 6-furfuryl aminopurine (KIN) 0.5 mg/l + 2,4-dichlorophenoxy acetic acid (2, 4-D) 2.0 mg/l was found to be bestfor mean callus induction (86%) in leaf explants after 6 weeks of culture and in case of shoot apex expant the best response and growth of callusing was observed on MS medium supplemented with 2,4-D 1.0 mg/l + BAP 2.0 mg/l (77%).The response of callus growth increases

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9

Chée, Raymond P., Jonathan R. Schultheis, and Daniel J. Cantliffe. "Plant Recovery from Sweetpotato Somatic Embryos." HortScience 25, no. 7 (1990): 795–97. http://dx.doi.org/10.21273/hortsci.25.7.795.

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Plant formation from somatic embryos in response to BAP, NAA, and sucrose was studied in sweetpotato [Ipomoea batatas (L.) Lam.]. A maximum of 15% embryos at the torpedo stage of development formed plants of agar-solidified basal medium containing 3% sucrose and no growth regulators. The percentage of embryos forming shoots was increased to 53% by 4 μm BAP, but BAP reduced whole plant formation and promoted callusing at the root axis end of embryos. The frequency of plant development was increased to 38% by adding 0.1 μm NAA to the basal medium. Reducing sucrose concentration to 1.6% in basal

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10

N, BABU, SHEEBA A, YOGAMEENAKSHI P, ANBUMALARMATHI J, and RANGASAMY P. "Effect of media composition on in vitro multiplication of sugarcane varieties." Madras Agricultural Journal 90, December (2003): 643–46. http://dx.doi.org/10.29321/maj.10.a00152.

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The in vitro response of sugarcane varieties CO 86249 and CO 8021 was assessed on twelve media for callus induction, multiplication, shooting and rooting. CO 86249 recorded the highest callusing frequency at 2,4-D 3 mg/lit. with 0.1 mg/ lit casein hydrolysate followed by 2,4-D 3 mg/lit with 100 ml of coconut milk. CO 86249 had significant shoot multiplication ratio and length in MS media supplemented with1 mg/lit IBA+ 2.0 mg/lit+1.0 mg/lit kinetin. The half strength MS media supplemented with 1 mg/lit NAA+ 1.25 mg/lit IBA was promoted profuse root in both the varieties. Whereas, full strength

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11

Viji, M., and A. Maruthupandian. "Metabolic Conditioning of Seedlings and its Effect on the Callusing Response in Green Gram, (Vigna radiata) L. Wilczek." International Journal of Advances in Interdisciplinary Research 4, no. 3 (2017): 35. http://dx.doi.org/10.29191/ijaidr.2017.4.3.07.

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Currey, Christopher J., Veronica A. Hutchinson, and Roberto G. Lopez. "Growth, Morphology, and Quality of Rooted Cuttings of Several Herbaceous Annual Bedding Plants Are Influenced by Photosynthetic Daily Light Integral During Root Development." HortScience 47, no. 1 (2012): 25–30. http://dx.doi.org/10.21273/hortsci.47.1.25.

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Cuttings of herbaceous annual bedding plants must be rooted in late winter and early spring when ambient outdoor photosynthetic daily light integrals (DLIs) are at seasonally low levels. We evaluated the effect of DLI during root development on growth, morphology, and quality of nine popular vegetatively propagated annual bedding plant species. Cuttings of Angelonia angustifolia Benth. ‘AngelMist White Cloud’, Argyranthemum frutescens (L.) Sch. Bip. ‘Madeira Cherry Red’, Diascia barberae Hook. f. ‘Wink Coral’, Lantana camara L. ‘Lucky Gold’, Nemesia fruticans (Thunb.) Benth. ‘Aromatica Royal’,

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13

Hettiarachchi, H. D. B. K., V. R. M. Vidhanaarachchi, S. P. N. C. Jayarathna, and Perera Dinum. "Effect of exogenous polyamines on coconut (<em>Cocos nucifera</em> L.) embryogenic callus multiplication." COCOS 23, no. 1 (2022): 47–56. http://dx.doi.org/10.4038/cocos.v23i1.5823.

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Major bottlenecks of coconut in vitro culture are poor plant regeneration rate, severe browning and premature necrosis of cultured tissue, and heterogeneous response of individual palms and explants to in vitro culture conditions. Among them, tissue browning is a common and often severe problem in coconut in vitro culture systems which results in death of explant/ callus ultimately. This experiment was carried out to enhance the in vitro multiplication of coconut, which is a highly recalcitrant species to in vitro culture through exogenously added polyamines in the media. The polyamines are im

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14

Cyriac, Angeline, Toji Thomas, and T. Dennis Thomas. "Mass Multiplication of Gymnema sylvestre (Retz.) R. Br. ex Schult. Through In vitro Shoot Organogenesis from Callus." Plant Tissue Culture and Biotechnology 30, no. 1 (2020): 27–32. http://dx.doi.org/10.3329/ptcb.v30i1.47788.

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For large scale production through shoot organogenesis from callus, seeds of Gymnema sylvestre were cultured on MS containing various concentrations of 2,4-D. An optimum callusing (62.40%) was observed on MS with 1.5 mg/l 2,4-D. Upon transfer to multiplication medium, the calli multiplied 2.5 times in 45 days. The calli were then tested for shoot induction and an optimum result in terms of both per cent response (52.04%) and an average number of shoots (47.2) were observed on MS supplemented with 1.0 mg/l BAP. Maximum shoot elongation was obtained on 0.5 mg/l Kn and best rooting took place on

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15

Ault, James Robert, and Sandy S. Siqueira. "Morphogenetic Response of Lilium michiganense to Four Auxin-type Plant Growth Regulators In Vitro." HortScience 43, no. 6 (2008): 1922–24. http://dx.doi.org/10.21273/hortsci.43.6.1922.

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Shoot, root, and callus induction were examined in the North American lily Lilium michiganense in response to treatment with four auxin-type plant growth regulators (PGR). Seed from controlled crosses were aseptically excised from slightly immature capsules and cultured in vitro on Murashige and Skoog basal medium and vitamins with 30 g·L−1 sucrose, 7.0 g·L−1 agar, and a pH = 5.7. Seed were maintained at 20 °C with a 14-h photoperiod. After 5.0 to 5.5 months, leaves and roots were removed from seedlings, the bulbs transversely sectioned, then the bulb sections cultured cut-surface down on the

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16

Ault*, Jim, and Sandy Siqueira. "Morphogenetic Response of Lilium michiganense to Four Auxins In Vitro." HortScience 39, no. 4 (2004): 813B—813. http://dx.doi.org/10.21273/hortsci.39.4.813b.

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Shoot, root, and callus induction were examined in the North American lily, Lilium michiganense, in response to treatment with four auxins. Seed from controlled crosses were aseptically excised from slightly immature capsules and cultured in vitro on Murashige and Skoog basal medium and vitamins with 30 g/l sucrose, 7.0 g/l agar, and a pH = 5.7. Seed were maintained at 20 °C with a 14-h photoperiod. After 5.0-5.5 months, leaves and roots were removed from seedlings, the bulbs transversely sectioned, then the bulb sections cultured cut-surface down on the identical medium supplemented with 0.0,

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17

Ribeiro, Juliana Martins, Silvio Lopes Teixeira, Joselita Cardoso de Souza, et al. "In vitro response of date palm (Phoenix dactylifera L.) inflorescence explants to high 2iP and 2,4-D concentrations." MAY 2020, no. 14(05):2020 (May 20, 2020): 831–35. http://dx.doi.org/10.21475/ajcs.20.14.05.p2317.

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One of the major problems related to the implementation of date palm crops in Brazil is propagation. Therefore, the aim of this study was to evaluate the possibility of using tissue culture technique for the in vitro propagation of this species. Hence, the effect of 2iP and 2,4-D on the in vitro response of date palm inflorescence tissues, related to floral bud swelling, callusing, and rhizogenesis, was evaluated. The absence of 2,4-D was more detrimental to the in vitro response of inflorescence bud explants than absence of 2iP. In treatments without addition of 2,4-D to the culture medium, e

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18

Pattnaik, Snigdha Sameer, Byomkesh Dash, Sudhansu Sekhar Bhuyan, et al. "Anther Culture Efficiency in Quality Hybrid Rice: A Comparison between Hybrid Rice and Its Ratooned Plants." Plants 9, no. 10 (2020): 1306. http://dx.doi.org/10.3390/plants9101306.

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An immense increase in human population along with diminished lands necessitates the increase of rice production since, it serves the human population as a staple food. Though rice hybrids (RH) are showing considerable yield enhancement over inbreds in terms of both quality and quantity, farmers’ adoption of hybrid rice technology has been much slower than expected because of several constraints such as seed cost and quality. Doubled haploid (DH) technology was considered useful for the development of inbred lines from rice hybrids in a single generation. Androgenesis shows its significance in

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19

SHITTU, H. O., G. C. MGBEZE, G. O. OKOLOKO, O. ASEMOTA, and C. R. EKE. "IN VITRO ASSESSMENT OF EMBRYO AGE AND NAPHTHALENE ACETIC ACID SUPPLEMENTATION OF MEDIA IN CALLUS GENERATION OF OIL PALM (Elaeis guineensis Jacq)." Nigerian Journal of Life Sciences (ISSN: 2276-7029) 3, no. 1 (2022): 179–86. http://dx.doi.org/10.52417/njls.v3i1.121.

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Callus generation from embryo explants of the oil palm (Elaeis guineensis Jacq) was observed in relation to age of plants at time of explants excision and types of media used. The two stages of explants were those at 15 and 18 weeks after anthesis. Eeuwens and Murashige & Skoog‘s (MS) media supplemented with Naphthalene acetic acid (NAA) were used. These gave two media variations viz: MS medium supplemented with NAA and Eeuwens‘ medium supplemented with NAA. The best combination for the generation of callus from the embryo explants within 56 days in culture was determined by observing type

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Rafiq, Shah, Nasir Aziz Wagay, Irshad Ahmad Bhat, et al. "In Vitro Propagation of Aconitum chasmanthum Stapf Ex Holmes: An Endemic and Critically Endangered Plant Species of the Western Himalaya." Horticulturae 7, no. 12 (2021): 586. http://dx.doi.org/10.3390/horticulturae7120586.

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Aconitum chasmanthum Stapf ex Holmes, a highly valued medicinal plant, is a critically endangered plant species with restricted global distribution. Because there is no published report on the in vitro micropropagation of A. chasmanthum, the present study was undertaken to contribute to the development of an efficient micropropagation protocol for its conservation. Seeds collected from the wild showed enhanced germination after being given a chilling treatment (−4 °C and −20 °C) for different durations (10, 20, 30 and 40 days). Seeds given a chilling treatment of −4 °C for 10 days showed enhan

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21

Naseer, Samar, and Tariq Mahmood. "Tissue culture and genetic analysis of somaclonal variations of Solanum melongena L. cv. Nirrala." Open Life Sciences 9, no. 12 (2014): 1182–95. http://dx.doi.org/10.2478/s11535-014-0354-6.

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AbstractThe present study was designed to analyze genetically somaclonal variants using biochemical and molecular markers. Efficient tissue culture protocol for Solanum melongena L. cv. Nirrala was developed. Maximum callus induction (100%) was observed for Murashige and Skoog (MS) media supplemented with 2.0 mg L−1 naphthalene acetic acid +0.5 mg L−1 6-benzylaminopurine; and nodal explants gave best callusing response (88.8%) as compared to internodes (88.3%) and leaves (87.7%). The best shooting was induced on nodal and internodal callus in the presence of 2.0 mg L−1 6-benzylaminopurine. Tot

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22

Murugesan, Dhandapani, Geethanjali Subramanian, Arumugam Palanivelu Salini, and Raveendran Muthurajan. "Optimization of high frequency plant regeneration protocol via embryogenic callus formation from diverse indica rice genotypes induced by combinations of 2,4-D and BA." Oryza-An International Journal on Rice 59, no. 3 (2022): 281–91. http://dx.doi.org/10.35709/ory.2022.59.3.3.

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A high frequency plant regeneration protocol via induction of embryogenic callus followed by somatic embryogenesis was standardized for a group of indica rice varieties consisting of land races and improved varieties, which are generally considered recalcitrant. Scutellum derived embryogenic calluses were obtained from the mature seeds inoculated into MS medium supplemented with different concentrations and combinations of 2,4-D and BA added with casein hydrolysate (500 mg/l) and proline (1 g/l). The varieties significantly differed in their response to embryogenic callusing and regeneration.

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23

Arias Padró, Maria D., Emilia Caboni, Karla Azucena Salazar Morin, Marco Antonio Meraz Mercado, and Víctor Olalde-Portugal. "Effect of Bacillus subtilis on antioxidant enzyme activities in tomato grafting." PeerJ 9 (March 12, 2021): e10984. http://dx.doi.org/10.7717/peerj.10984.

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Grafting generally means stress to a plant and this triggers antioxidant defense systems. An imbalance in reactive oxygen species may negatively affect the grafting success. Several research projects have studied the association with plant growth-promoting rhizobacteria (PGPR) and it has been documented that they enhance nutrient acquisition, regulate hormone levels, and influence the antioxidant response in crops. However, little is known about the strategy of inoculating grafted herbaceous plants with PGPR and its effect on the antioxidant response. The effects of inoculating a strain of Bac

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Smitha, Devi P. S., T. S. Preetha, and A. S. Hemanthakumar. "Establishment of an efficient in vitro propagation system for Holostemma annulare (Roxb.) K. Schum. using SH medium." Research Journal of Biotechnology 17, no. 2 (2022): 118–24. http://dx.doi.org/10.25303/1702rjbt118124.

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Holostemma annulare (Roxb.) K. Schum. (Asclepiadaceae) is an important medicinal herb and its root tubers are an important ingredient of the drug ‘jivanti’. Here, a more reliable procedure of in vitro propagation of the plant is discussed using SH nutrient medium formulation that facilitates the in vitro multiplication and establishment without callusing from the basal region of shoots that was prominent in the earlier protocols. Surface sterilization of the nodal explants with 10% (v/v) NaOCl for 12 minutes followed by 0.1% (w/v) HgCl2 for 8 minutes exhibited 90% shooting response. The in vit

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25

Afrin, S., MA Rahman, M. Khalekuzzaman, MM Hasan, AHF Fahim, and MA Alam. "Study on in vitro micropropagation of Rosa sp." Bangladesh Journal of Agriculture 47, no. 1 (2022): 66–74. http://dx.doi.org/10.3329/bjagri.v47i1.60593.

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Tissue culture has long been recognized as an ideal experimental paradigm for studying the mechanisms governing plant cell growth, division, and physiological and biochemical processes. The present investigation was undertaken to establish a standard method for callus induction, shoot and root regeneration of rose at Genetic Engineering and Biotechnology Laboratory, University of Rajshahi, Bangladesh in 2010. Shoot tip and nodal segments were used as experimental materials excised from field grown plants for callus induction. The direct shoot was observed from both shoot tip and nodal segments

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26

Currey, Christopher J., and Roberto G. Lopez. "Biomass Accumulation and Allocation, Photosynthesis, and Carbohydrate Status of New Guinea Impatiens, Geranium, and Petunia Cuttings Are Affected by Photosynthetic Daily Light Integral during Root Development." Journal of the American Society for Horticultural Science 140, no. 6 (2015): 542–49. http://dx.doi.org/10.21273/jashs.140.6.542.

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During the propagation of herbaceous stem-tip cuttings, the photosynthetic daily light integral (DLI) inside greenhouses can be low (≈1–4 mol·m−2·d−1) during the winter and early spring when propagation typically occurs. The mechanisms by which cuttings adapt biomass allocation patterns, gas exchange, and starch accumulation in response to the photosynthetic DLI are not clearly understood. Our objectives were to quantify the impact of DLI on growth, photosynthesis, and carbohydrate concentration during the root development phase of cutting propagation. Petunia (Petunia ×hybrida ‘Suncatcher Mid

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Dreger, Mariola, Milena Szalata, Małgorzata Górska-Paukszta, Grażyna Mańkowska, and Karolina Wielgus. "Effect of Auxin Transport Inhibitors on Shoot Organogenesis of Hemp (Cannabis sativa L.) Epicotyl Explants." Biology and Life Sciences Forum 4, no. 1 (2020): 15. http://dx.doi.org/10.3390/iecps2020-08843.

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Industrial hemp (Cannabis sativa L.) is an economically valuable crop used in the production of nutraceutical supplements, functional food, pharmaceuticals, cosmetics, etc. However, the large-scale propagation of this plant has been limited by challenges regarding its low regeneration rate and the variety-/genotype-dependent response of the explants. Previously, it was shown that elevated endogenous auxin levels are inhibitory for shoot organogenesis and the use of auxin transport inhibitors may improve shoot regeneration in some recalcitrant species. This study explored the effect of the appl

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Tsipouridis, C., and T. Thomidis. "Rooting of 'GF677' (almond × peach hybrid) hardwood cuttings in relation to hydrogen hyperoxide, moisture content, oxygen concentration, temperature and pH of substrate." Australian Journal of Experimental Agriculture 44, no. 8 (2004): 801. http://dx.doi.org/10.1071/ea03059.

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In this study, the influence of water content, temperature, oxygen concentration and pH of perlite substrate and H2O2 treatments on the rooting percentage of GF677 hardwood cuttings was examined. The best moisture level was 100 mL in 500 mL perlite; this resulted in 100% rooting. The observations showed that even pure O2 did not reduce callusing or, possibly, rooting and it gave similar results as 21% O2. There was no indication of a promotory effect. No rooting or callusing was observed in N, 5% CO2 or 5% O2 treatments. There were significant differences in the responses to temperature and pH

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Liedtke, Jenny, Muhali Jimoh, and Charles Laubscher. "Rooting Responses of Female and Male Cuttings of Leucadendron elimense E. Phillips subsp. elimense on the Type and Concentration of Auxin." Journal of Horticultural Research 29, no. 2 (2021): 57–64. http://dx.doi.org/10.2478/johr-2021-0016.

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Abstract Female and male plants of difficult-to-root species Leucadendron elimense subsp. elimense were investigated for rooting potential with three rooting hormones: indole-3-acetic acid (IAA), 1-naphthaleneacetic acid (NAA), and indole-3-butyric acid (IBA) at three different concentrations (2000 ppm, 4000 ppm, and 6000 ppm). The experiment was made under essential misting, bottom heat, and a naturally ventilated greenhouse. After 18 weeks the experiment terminated and callusing and rooting percentage, root number, and root length were determined. Female cuttings rooted in the highest percen

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Fufa, Hundessa, and Jiregna Daksa. "Development of an in vitro Callus Induction Protocol and Shoot Proliferation for Selected Jatropha (Jatropha curcas) Accessions." Plant Tissue Culture and Biotechnology 30, no. 1 (2020): 131–41. http://dx.doi.org/10.3329/ptcb.v30i1.47798.

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The present study was undertaken to establish a protocol for in vitro callusing of three Jatropha accessions, namely Metema, Adami Tulu and Shewa Robit from leaf explants. The medium supplemented with combination of 4.44 μM BAP and 4.52 μM 2,4-D resulted in maximum percentage of callus (100%) formed for all accessions. The maximum shoot regeneration (66.67%) from callus with 10.13 number of shoot was obtained from Shewa Robit in MS medum fortified with TDZ (2.27 μM ) and IBA (0.49 μM ). The presence of TDZ in the shoot regeneration medium has greater influence on the induction of adventitious

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Kumar, Sunil, Virendra Kumar, Manoj Kumar Sharma, et al. "Effects of different plant growth regulators on in vitro callus induction in physic nut (Jatropha curcus L.)." Journal of Applied and Natural Science 7, no. 1 (2015): 30–37. http://dx.doi.org/10.31018/jans.v7i1.559.

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Jatropha (Jatropha curcas L.) is an oil bearing crop growing in tropical and subtropical parts of the world. The present study was undertaken to investigate the effects of different plant growth regulators on in vitro callus induction in physic nut (J. curcus). In the present study, it was observed that all the explants viz., leaf lamina, petioles, nodal segments and cotyledonary nodes showed good callus induction responses on various culture media thus tried. Leaf lamina and petioles showed 100.0% callus induction responses on different MS media supplemented with auxins and cytokinins alone o

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32

Frankenstein, Claus, and Uwe Schmitt. "Wound effects in the xylem of poplar: A UV microspectrophotometric study." Holzforschung 60, no. 6 (2006): 595–600. http://dx.doi.org/10.1515/hf.2006.101.

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Abstract Cell wall modifications in vessels and fibres of wound wood of Populus tremula L.×P. tremuloides Michx. formed after mechanical wounding have been examined by light microscopy, transmission electron microscopy, and UV microspectrophotometry (in scanning and point measurement mode), mainly focusing on the lignin distribution. With this goal, wound xylem within lateral wound callusis was collected after response periods of up to 23 months. Vessels and fibres in wound xylem deviated from their usual axial orientation. Vessels within the wound xylem were smaller in diameter and shorter in

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El, Goumi Y., S. Essayagh, M. Fakiri, M. Benbachir, and O. Lamsaouri. "Effect of Cold Pretreatment, Anthers Orientation, Spikelet Position and Donor Tiller on the Callusing Response in Barley Anther In vitro Culture." October 11, 2017. https://doi.org/10.19070/2379-1020-SI02003.

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The anthers in vitro culture is a useful technique for plant improvement as to create new varieties. However, the success of this technique is limited by several factors. This study was carried out to identify factors as the influence of spikelet position in the spike, position of donor tiller on plate tillering and anther orientation onto the medium as well as cold pretreatment and the genotype that affect the callusing induction in anthers in vitro culture of three cultivars of Moroccan barley (Arig 8, Asni and Tamelalt). Among genotypes, the callusing response was significantly higher in As

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Deepti, Swati Rani, Kumari Anjani, Rajiv Kumar, and Vinay Kumar Sharma. "In-vitro Screening for Disease Resistance in Wheat Genotypes against Bipolaris sorokiniana Using Callus Culture Method." Current Journal of Applied Science and Technology, December 31, 2020, 82–87. http://dx.doi.org/10.9734/cjast/2020/v39i4631178.

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The present investigation was done to identify the efficacy of callus culture method for in-vitro screening to identify resistant and susceptible wheat genotypes. The study led to establishment of protocol for in-vitro screening of susceptible and resistant wheat genotypes against Bipolaris sorokiniana. The Bipolaris sorokiniana crude toxin was used at various concentrations to supplement the callusing medium and the response of twelve genotypes was studied. The susceptible genotype Agra local showed maximum area prone to death of the callus because of effect of toxin in medium supplemented wi

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Subedi, M., B. R. Ojha, S. K. Ghimire, et al. "Effect of sugarcane genotypes and 2, 4-dichlorophenoxy acetic acid on callus induction." Journal of the Institute of Agriculture and Animal Science, August 10, 2018, 237–42. http://dx.doi.org/10.3126/jiaas.v33i0.20710.

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Callus induction is an important step in in-vitro culture valued for micropropagation, somaclonal variation, mutagenesis, synthetic seed production, transformation and genetic engineering. Callusing response of ten sugarcane genotypes viz., BO-110, BO-134, Cose-95422, Cose-98232, Cose-92423, UP-9742, BO-91, BO-135, and Cose-98255, were studied under three different doses (2, 3 & 4 mg L-1) of 2,4-D supplemented in the callus induction media. Callus induction was found to depend on both genotype and 2,4-D concentration in the media. The genotype UP-9742 was most amenable to callus induction

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Y, El Goumi, and Essayagh S. "Effect of Cold Pretreatment, Anthers Orientation, Spikelet Position and Donor Tiller on the Callusing Response in Barley Anther In vitro Culture." International Journal of Medical Biotechnology & Genetics, October 11, 2017, 33–38. http://dx.doi.org/10.19070/2379-1020-si02003.

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Sultana, R. S., N. Ahsan, R. Islam, M. R. Ali, M. Anisuzzaman, and M. F. Alam. "EVALUATION OF SOMACLONES OF POTATO (SOLANUM TUBEROSUM L.)." Khulna University Studies, October 23, 2022, 43–47. http://dx.doi.org/10.53808/kus.2005.6.1and2.0329-l.

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Evaluation of somaclones in the field of three potato varieties (Diamant, Multa and Cardinal) were examined through callus culture using node, internode and leaf segments. Multiple shoot regeneration from callus at various frequencies was observed using different concentrations and combinations of growth regulators. The highest percentage of callus induction from internode was observed in MS medium containing 3.0 mg l-1 of 2,4-D + 150 mg l-1 of L-asparagine. In this combination, the percentage of callusing was 90% for Diamant, 80% for Multa and 70% for Cardinal. The best response for multiple

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Okazaki, Karin, Imari Koike, Sayuri Kera, et al. "Gene expression profiling before and after internode culture for adventitious shoot formation in ipecac." BMC Plant Biology 22, no. 1 (2022). http://dx.doi.org/10.1186/s12870-022-03756-w.

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Abstract Background In ipecac (Carapichea ipecacuanha (Brot.) L. Andersson), adventitious shoots can be induced simply by placing internodal segments on phytohormone-free culture medium. The shoots form locally on the epidermis of the apical region of the segments, but not the basal region. Levels of endogenous auxin and cytokinin transiently increase in the segments after 1 week of culture. Results Here, we conducted RNA-seq analysis to compare gene expression patterns in apical and basal regions of segments before culture and after 1 week of culture for adventitious shoot formation. The resu

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Battiston, Enrico, Sara Falsini, Alessio Giovannelli, et al. "Xylem anatomy and hydraulic traits in Vitis grafted cuttings in view of their impact on the young grapevine decline." Frontiers in Plant Science 13 (October 5, 2022). http://dx.doi.org/10.3389/fpls.2022.1006835.

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Grapevine grafting is an essential practice in viticulture and over the years, various bench grafting techniques have been developed to mechanize the nursery process and to increase the yield in number of viable cuttings. Bench grafting is a fundamental nursery practice that can potentially affect the quality of propagation material also in young decline associated to grapevine trunk diseases and has been recently reported to influence leaf symptoms development associated with diseases of Esca complex. The study aimed to investigate how three bench grafting methods [i.e., (i) Omega graft as me

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Mahood, Huda E., Virginia Sarropoulou, and Thiresia-Teresa Tzatzani. "Effect of explant type (leaf, stem) and 2,4-D concentration on callus induction: influence of elicitor type (biotic, abiotic), elicitor concentration and elicitation time on biomass growth rate and costunolide biosynthesis in gazania (Gazania rigens) cell suspension cultures." Bioresources and Bioprocessing 9, no. 1 (2022). http://dx.doi.org/10.1186/s40643-022-00588-2.

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AbstractGazania rigens (L.) Gaertn. (Asteraceae) is a medicinal plant with high ornamental potential and use in landscaping. The therapeutic potential of sesquiterpene lactones (SLs) as plant natural products for pharmaceutical development has gained extensive interest with costunolide (chemical name: 6E,10E,11aR-6,10-dimethyl-3-methylidene-3a,4,5,8,9,11a-hexahydrocyclodeca[b]furan-2-one) used as a popular herbal remedy due to its anti-cancer, antioxidant, anti-inflammatory, anti-microbial, anti-allergic, and anti-diabetic activities, among others. In the present study, two explant types (leaf

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Chamandoosti, Firoozeh. "Effect of Cytokinins on the Vegetative Propagation of Olea europaea L. (cv. Koroneiki)." PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, August 3, 2023, 3–4. http://dx.doi.org/10.56557/pcbmb/2023/v24i3-48330.

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The study was carried out in soil born diseases laboratory, Iranian Research Institute of Plant Protection during Mar 2022 to Sep 2022. The Single node explants (prepared from young certificated scions from Fadak garden in Qom province) of Koroneiki olive scions cultured on DKW medium. Culture media were free from PGRs in establishment stage. After the mentioned stage, explant cultured on DKW media fortified with PGRs, in order to presentation a simple and repeatable method for shoot multiplication which is one of the important method for In Vitro olive propagation by examining three item incl

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Haque, Muhammad Shahidul, Md Hasanuzzaman, Md Tohidur Rahman, Nazmul Islam, Shamsun Nahar Begum, and Sabina Yasmin. "Hydroponic and in vitro screening of wheat varieties for salt-tolerance." Plant Science Today, July 21, 2022. http://dx.doi.org/10.14719/pst.1686.

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Salt-tolerant wheat cultivars are essential for sustainable wheat production and global food security. The present study aimed to establish a reliable screening protocol as well as successfully isolated the potential salt-tolerant wheat varieties by discerning morpho-physiological parameters with multivariate analysis. Seventeen wheat varieties were evaluated at 0, 12, 15 and 18 dSm-1 salinity levels in a hydroponic culture system at the seedling stage. Moreover, in vitro callusing responses of four selected varieties were determined to clarify the salt tolerance capability at 0, 9, 12 and 15

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Battiston, Enrico, Stéphane Compant, Livio Antonielli, et al. "In planta Activity of Novel Copper(II)-Based Formulations to Inhibit the Esca-Associated Fungus Phaeoacremonium minimum in Grapevine Propagation Material." Frontiers in Plant Science 12 (March 15, 2021). http://dx.doi.org/10.3389/fpls.2021.649694.

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Grapevine trunk diseases (GTDs) are a serious and growing threat to vineyards worldwide. The need for innovative control tools persists since pesticides used against some GTDs have been banned and only methods to prevent infections or to reduce foliar symptoms have been developed so far. In this context, the application of imaging methods, already applied to study plant–microbe interactions, represents an interesting approach to understand the effect of experimental treatments applied to reduce fungal colonization, on GTD-related pathogens activity. To this aim, trials were carried out to eval

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