Academic literature on the topic 'Campylobacter termofili / thermophilic Campylobacter spp'

Th ermophilic Campylobacter spp. are the leading cause of zoonotic enteric disease in Europe and USA. In Serbia, it has an upward trend in human population. Th e disease is usually indirectly transmitted to humans through the consumption of food contaminated by the faeces of infected animals. Th e aim of this paper was to analyze data on the prevalence of Campylobacter spp. in poultry meat production chain and the risk for the development of the disease in humans. Th e Campylobacter jejuni/coli was identifi ed at farm level in 73.3% of poultry, 66.6% calves and 58.3% pig samples of already ill or suspected cases. Clinical manifestation of the disease in birds can be expected if an additional immunosuppressive factor is present. Artifi cial infection of healthy chickens with 6.77 log cfu C. jejuni per chicken on day 21st of life leads to 5.26 log cfu/g faeces aft er only fi ve days with a tendency to decrease during the next 18 days. Although chilling and freezing may signifi cantly reduce Campylobacter contamination of carcasses, it cannot completely eliminate the initial contamination. According to our experimental results the prevalence of Campylobacter contaminated chickens from positive fl ock appears to drop from 100% live birds (with 3.02 log cfu/g faeces) to 50% of chicken carcasses. Contamination of the carcasses depends on initial contamination of live birds, good hygiene practices andgood manufacturing practices. Th erefore, high variability in contamination of carcasses can be considered; prevalence range from 11.43 to 90.00% ofcarcases was established in various slaughterhouses. At retail, Campylobacter was detected in 18.8% poultry meat samples and 10.0% samples of othermeat types. Campylobacter is frequently found in the entire production chain of poultry meat and represents high risk for consumers’ health.

ABSTRACT Thermophilic Campylobacter spp. are one of the primary causes of bacterial human diarrhea. The consumption of poultry meats, by-products, or both is suspected to be a major cause of human campylobacteriosis. The aims of this study were to determine the prevalence of thermophilic Campylobacter spp. in fresh poultry meat and poultry by-products by conventional culture methods and to confirm Campylobacter jejuni and Campylobacter coli isolates by using the multiplex PCR assay. Two hundred fifty fresh poultry samples were collected from a variety of supermarkets and slaughterhouses located in Sfax, Tunisia, including chicken (n =149) and turkey (n =101). The samples were analyzed using conventional microbiological examinations according to the 2006 International Organization for Standardization method (ISO 10272-1) for Campylobacter spp. Concurrently, a real-time PCR was used for identification of C. jejuni and C. coli. Of the 250 samples of poultry meat and poultry by-products, 25.6% (n = 64) were contaminated with Campylobacter spp. The highest prevalence of Campylobacter spp. was found in chicken meat (26.8%) followed by turkey meat (23.7%). Among the different products, poultry breasts showed the highest contamination (36.6%) followed by poultry by-products (30%), poultry wings (28%) and poultry legs (26%) showed the lowest contamination, and no contamination was found on neck skin. Of the 64 thermophilic Campylobacter isolates, C. jejuni (59.7%) was the most frequently isolated species and 10.9% of the isolates were identified as C. coli. All of the 64 Campylobacter isolates identified by the conventional culture methods were further confirmed by PCR. The seasonal peak of Campylobacter spp. contamination was in the warm seasons (spring and summer). The study concluded that high proportions of poultry meat and poultry by-products marketed in Tunisia are contaminated by Campylobacter spp. Furthermore, to ensure food safety, poultry meats must be properly cooked before consuming.

The prevalence of thermophilic Campylobacter spp. was evaluated in the caecum and on carcasses of pigs at slaughter and in the facilities of slaughterhouses in the period of 2001– 2003. During that timeframe, prevalence of Campylobacter spp. in both the pigs and the environment of slaughterhouses decreased. In 2001, Campylobacter spp. were detected in 34% of 316 samples; in 2002 there were 27% of positive findings out of the 624 samples; and in 2003, Campylobacter spp. were detected in 16% out of 300 samples. Campylobacter spp. were mostly found primarily in the caecum (292 isolates) and in smears collected from carcasses (21 isolates), while Campylobacter spp. were isolated only sporadically from the work surfaces of equipment in slaughterhouses. The majority of isolates were identified as C. coli. In 2001, 16 out of 109 strains of Campylobacter spp. were identified as C. jejuni; in 2002, 8 out of 167 strains were C. jejuni; and in 2003, none of 47 isolates was identified as C. jejuni.

The purpose of this study was to determine the presence of thermophilic Campylobacter spp. and Salmonella spp. in sand from non-EEC standard and EEC standard designated beaches in different locations in the UK and to assess if potentially pathogenic strains were present. Campylobacter spp. were detected in 82/182 (45%) of sand samples and Salmonella spp. in 10/182 (6%). Campylobacter spp. were isolated from 46/92 (50%) of samples from non-EEC standard beaches and 36/90 (40%) from EEC standard beaches. The prevalence of Campylobacter spp. was greater in wet sand from both types of beaches but, surprisingly, more than 30% of samples from dry sand also contained these organisms. The major pathogenic species C. jejuni and C. coli were more prevalent in sand from non-EEC standard beaches. In contrast, C. lari and urease positive thermophilic campylobacters, which are associated with seagulls and other migratory birds, were more prevalent in sand from EEC standard beaches. Campylobacter isolates were further characterized by biotyping and serotyping, which confirmed that strains known to be of types associated with human infections were frequently found in sand on bathing beaches.

Six commercially available anti-Campylobacter antibodies were examined for their applicability in an enzyme immuno-assay on hydrophobic grid membrane filters, both for the detection and enumeration of Campylobacter spp. When a panel of nine Campylobacter (seven Campylobacter jejuni and two Campylobacter coli) and eight non-Campylobacter strains were used in a dot-blot format enzyme immunoassay to test the specificity of these antibodies, only one polyclonal antibody (Biodesign) detected all Campylobacter strains. Escherichia coli O157:H7 produced weak nonspecific signals due to endogenous peroxidase activity. The specificity of this Biodesign antibody was further tested against 30 more Campylobacter strains and more than 600 non-Campylobacter strains on hydrophobic grid membrane filters grown on modified Campylobacter agar with charcoal and deoxycholate, a Campylobacter selective medium. All the Campylobacter strains were detected, whereas only two (Acinetobacter calcoaceticus, Salmonella Minnesota) of the approximately 130 non-Campylobacter strains, which grew on modified Campylobacter agar with charcoal and deoxycholate, gave false-positive signals. This simple, rapid, and specific enzyme immunoassay also detected Campylobacter spp. from inoculated milk and chicken rinses and naturally contaminated chicken rinses.

Assessing the carriage of Campylobacter in animal reservoirs is essential to better understand Campylobacter epidemiology. Here, we evaluated the prevalence of thermophilic Campylobacter spp. in dogs and cats, hereafter defined as pets, and characterized Campylobacter jejuni (C. jejuni) isolates to assess their genetic diversity and their potential link with isolates from other animals or human cases. During a 6-month period, 304 feces samples were collected from pets. A significantly higher prevalence of thermophilic Campylobacter spp. was found in dogs compared with cats, as well as in dogs ≤ 1-year-old compared with older dogs. C. jejuni was the predominant species found in pets, and its genomic characterization revealed a high genetic diversity. Genotypes comparison with previously characterized isolates revealed a partial overlap between C. jejuni isolates from pets, chicken, cattle, and clinical cases. This overlap suggests the potential role of livestock and humans in pets’ exposure to Campylobacter, or vice versa. The isolation of pets’ specific profiles may suggest the existence of other sources of pet contamination or imply that pets may constitute a reservoir for Campylobacter. Because of the proximity between humans and pets, along with their frequent carriage of C. jejuni, human exposure to Campylobacter from pets can be more important than previously thought.

The purpose of the present study was to determine the prevalence of thermophilic Campylobacter in poultry, pork, and beef meat at the retail level and to identify the main categories of meat representing the most significant reservoirs of Campylobacter. A monitoring study was conducted throughout Poland from 2009 to 2013. A total of 1,700 fresh meat samples were collected from supermarkets, large retail outlets, and smaller stores. Thermophilic Campylobacter species were detected in 690 (49.3%) of 1,400 poultry samples collected from retail trade. Strains were isolated from 50.2 and 41.1% of raw chicken and turkey meat samples, respectively, and from 50.1 and 42.6% of raw chicken and turkey giblets. The incidence of Campylobacter spp. on pork (10.6%) and beef (10.1%) was significantly lower than on poultry. Campylobacter jejuni was the most prevalent Campylobacter species in chicken (46.6%), pork (68.6%), and beef (66.7%), and Campylobacter coli was the most frequently isolated Campylobacter species in turkey meat (71.2%). This study revealed that retail raw meats are often contaminated with Campylobacter; however, the prevalence of these pathogens is markedly different in different meats. Raw retail meats are potential vehicles for transmitting foodborne diseases, and our findings stress the need for increased implementation of hazard analysis critical control point programs and consumer food safety education efforts.

Background: The objective of this study was to determine the prevalence of thermophilic Campylobacter spp. in asymptomatic school-going children and establish the antibiotic resistance patterns of the isolates towards the drugs used to treat campylobacteriosis, including macrolides, quinolones and tetracycline. Campylobacter spp. are a leading cause of enteric illness and have only recently shown resistance to antibiotics. Methods: This study isolated Campylobacter spp., including Campylobacter coli, Campylobacter jejuni and Campylobacter lari, in stool samples from asymptomatic school-going children in one of the biggest urban slums in Kenya. The disc diffusion method using EUCAST breakpoints was used to identify antibiotic-resistant isolates, which were further tested for genes encoding for tetracycline resistance using primer-specific polymerase chain reaction. Results: In total, 580 stool samples were collected from 11 primary schools considering both gender and age. Subjecting 294 biochemically characterized Campylobacter spp. isolates to genus-specific PCR, 106 (18.27% of stool samples) isolates were confirmed Campylobacter spp. Out of the 106 isolates, 28 (4.83%) were Campylobacter coli, 44 (7.58%) were Campylobacter jejuni while 11 (1.89%) were Campylobacter lari. Campylobacter jejuni had the highest number of isolates that were multi-drug resistant, with 26 out of the 28 tested isolates being resistant to ciprofloxacin (5 mg), nalidixic acid (30 mg), tetracycline (30 mg) and erythromycin (15 mg). Conclusions: In conclusion, asymptomatic school going children in the study area were found to be carriers of multidrug resistant Campylobacter coli, Campylobacter jejuni and Campylobacter lari at 84%. A one-health approach, which considers overlaps in environment, animals and human ecosystems, is recommended in addressing multidrug resistane in Campylobacter, since animals are the main reservoirs and environmental contamination is evident.

Campylobacteriosis, a human infection caused by thermophilic Campylobacter spp. (particularly C. jejuni and C. coli), is the most commonly reported zoonosis in the European Union and one of the main bacterial causes of human gastroenteritis worldwide. Domestic poultry are the main reservoir of these microorganisms and harbour them without clinical manifestations. Handling and consumption of contaminated poultry meat has been reported as main source of human infection. Although campylobacteriosis is generally a self-limiting illness, severe cases require antimicrobial treatment, with macrolides and fluoroquinolones used as first-line therapy, while tetracyclines are second-choice drugs. Nowadays resistance to these antimicrobials is increasingly observed in C. jejuni and C. coli, determining a worldwide concern for public health, since resistant strains can be easily transmitted from animals to humans via the food-chain. Despite the high number of commercial broiler and turkey farms in Northern Italy and the economic importance of this sector in the Italian animal production, few on-farm studies have been carried out to investigate on the colonization of poultry with thermophilic Campylobacter spp. in this area. Considering that, together with the remarkable public health implications of the presence of Campylobacter spp. in poultry farms, the present study was aimed at determining the occurrence, the genetic diversity and the antimicrobial resistance of thermophilic campylobacters in the two leading poultry species reared for meat production in Italy, i.e. broilers and meat turkeys. A total of 10 broiler flocks reared in 7 different farms in Veneto and Lombardy regions and 3 turkey flocks belonging to 3 farms located in Veneto region were examined for the presence of thermophilic Campylobacter spp. by collecting cloacal swabs from randomly selected birds. Broiler flocks were sampled once, while turkeys were monitored with several samplings for the whole production cycle in order to evaluate the development of the colonization over time, because it has not been deeply studied in turkeys. Campylobacter isolates were genetically characterized by flaA-SVR typing and then examined for their phenotypic antimicrobial resistance to a wide range of antimicrobial drugs by the disk diffusion method. The occurrence of class 1 and class 2 integrons was also investigated. A wide colonization by C. jejuni and C. coli was observed both in broiler flocks (7 positive out of 10) and in turkey flocks (all positive), with differences among flocks in the Campylobacter species distribution, although an overall dominance of C. jejuni was detected. Interestingly, the onset of colonization differed considerably among turkey flocks, but in all of them it persisted up to the time of slaughter. A high genetic diversity among isolates was detected both in broilers and turkeys, with most genotypes showing a flock- or farm-specificity. Most strains isolated from both avian species were resistant to a high number of antimicrobials, including those of first and second choice for human campylobacteriosis therapy. Conversely, either class 1 or class 2 integrons were not detected. However, it should be noted that differences between broilers and turkeys were detected both in genotypes colonizing them and in antimicrobial resistance, suggesting discrepancies among the two poultry species in the epidemiological dynamics of Campylobacter spp. Overall, this study provides new insights into the epidemiology of thermophilic Campylobacter spp. in commercial broilers and meat turkeys in Northern Italy and suggests that they may represent a concrete risk for the transmission of multidrug-resistant strains to humans in our country
La campilobatteriosi, un’infezione umana provocata dalle specie termofile di Campylobacter (soprattutto da C. jejuni a C. coli), è la zoonosi più frequentemente riportata nell’Unione Europea e una delle principali cause di gastroenterite batterica nell’uomo a livello mondiale. Le specie avicole domestiche sono il principale reservoir di questi microrganismi, dei quali sono carrier asintomatici, e infatti la manipolazione e il consumo di carne avicola contaminata rappresentano le principali fonti d’infezione per l’uomo. Nonostante la campilobatteriosi sia una malattia autolimitante, nei casi gravi è necessario un trattamento antimicrobico. Macrolidi e fluorochinoloni costituiscono la terapia d’elezione, seguiti dalle tetracicline come molecole di seconda scelta. Attualmente il fenomeno dell’antimicrobico-resistenza è sempre più comune in C. jejuni e in C. coli, e in tutto il mondo rappresenta motivo di grande preoccupazione per la sanità pubblica, poiché ceppi antimicrobico-resistenti possono essere trasferiti dagli animali all’uomo attraverso gli alimenti. Nonostante l’elevata concentrazione di allevamenti intensivi di polli e tacchini da carne nel Nord Italia e l’importanza economica di questo settore nel comparto delle produzioni animali italiane, sono poche le indagini svolte negli allevamenti di quest’area geografica per valutare la presenza di Campylobacter termofili a livello di produzione primaria. Alla luce di questo e delle importanti implicazioni di sanità pubblica della presenza di questi microrganismi zoonotici negli allevamenti avicoli, questo studio è stato intrapreso allo scopo di valutare la presenza, la diversità genetica e l’antimicrobico-resistenza di Campylobacter termofili nelle due specie avicole da carne maggiormente allevate in Italia: polli broiler e tacchini. In totale sono stati esaminati 10 gruppi di broiler di 7 diversi allevamenti di Veneto e Lombardia, e 3 gruppi di tacchini di 3 allevamenti veneti. In ciascun gruppo di animali sono stati eseguiti tamponi cloacali a soggetti scelti causalmente. In ciascun gruppo di broiler è stato eseguito un campionamento, mentre nei tacchini sono stati svolti numerosi campionamenti per valutare l’andamento della colonizzazione durante tutto il ciclo produttivo, aspetto poco studiato in questa specie avicola. I Campylobacter isolati sono stati caratterizzati geneticamente mediante flaA-SVR typing e quindi sottoposti ad antibiogramma secondo il metodo della diffusione in agar per valutarne la sensibilità a più antimicrobici. Inoltre è stata valutata la presenza di integroni di classe 1 e 2. È stata rilevata una notevole diffusione di C. jejuni e C. coli sia nei broiler (7 gruppi positivi su 10), sia nei tacchini (positività di tutti e 3 i gruppi), con differenze tra i gruppi nella distribuzione delle due specie termofile e una complessiva predominanza di C. jejuni. Nei gruppi di tacchini la positivizzazione è avvenuta in tempi molto diversi, ma in tutti è persistita fino al termine del ciclo produttivo. É stata riscontrata una notevole diversità genetica nei ceppi isolati sia dai broiler, sia dai tacchini e la maggior parte dei genotipi era esclusiva di un gruppo di animali o di un allevamento. Inoltre, la maggioranza dei ceppi isolati da entrambe le specie avicole era resistente a molti antimicrobici, compresi quelli di prima e seconda scelta nella terapia della campilobatteriosi. Al contrario, non sono stati riscontrati integroni né di classe 1 né di classe 2. É importante notare che sono state riscontrate differenze tra broiler e tacchini sia relativamente ai genotipi dai quali erano colonizzati, sia nell’antimicrobico-resistenza, riscontri che suggeriscono il sussistere di differenze tra le popolazioni di Campylobacter che colonizzano l’una e l’altra specie avicola. Nel complesso, questo studio apporta nuovi elementi sull’epidemiologia di Campylobacter spp. in polli e tacchini da carne del Nord Italia, che possono rappresentare un rischio concreto per la trasmissione di ceppi multi-resistenti all’uomo

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Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq
Poultry products are recognized as sources of contamination by several pathogens. In the last decades the genus Campylobacter, particularly thermophilic species, stands out in developed countries, where it is considered the primary agent responsible for Foodborne Illnesses (DVA's). Due to the importance of this pathogen in public health and economics, researches are conducted in order to identify and characterize the isolated, as well as develop and improve techniques for the identification of the genus Campylobacter spp. with attention focused mainly to important thermophilic species related to food, allowing the determination of the prevalence of this pathogen in main placement sources, such as chicken. The objective of this study was to verify the occurrence of Campylobacter spp. and identify isolated thermophilic species from chicken carcasses in slaughterhouses in the state of Goiás submitted to the Federal Inspection Service which had proper authorization to internal and external trade. A total of 200 samples have been evaluated using the immunoenzymatic screening assay, with subsequent bacterial samples isolation and identification through biotyping. In this study it was observed that the employed methods allowed the isolation of Campylobacter spp. in 24% (48/200) of the assessed samples. Among these samples, 21 (10.5%) were demonstrably positive for genre, having as basis the set of analytical screening method, sequential bacterial isolation for positive samples and evidence of enzymatic and biochemical assimilation and antimicrobial profile. C.coli was the most frequently isolated species (58.6%), followed by C.jejuni ssp. jejuni (17.2%), C.lari (13.8%) and Campylobacter jejuni ssp. doylei (3.4%). By using a screening method, conventional isolation system and numerical biotyping it was immediately revealed the characterization of the isolated Campylobacter. It was observed that some suggestive isolated, due to its positivity in the screening test, growth on selective sources, incubation temperature, motility and morphology, characteristics of the genus assessed, have not been identified by numerical system and thus were considered negative. From the results, it can be concluded that the association of screening method to conventional isolation and biotyping numerical profile allowed a greater understanding of the isolated. It can also be concluded that Campylobacter spp. is present in chicken meat for human consumption and three thermophilic species and two subspecies have been identified. The pathogen presence attests public health risk.
Os produtos de origem avícola podem veicular diversos microrganismos patogênicos. Nas últimas décadas, o gênero Campylobacter, em especial as espécies termofílicas, destaca-se como um dos principais agentes responsáveis por Doenças Veiculadas por Alimentos (DVA’s). Em decorrência da importância deste patógeno na saúde pública e na economia, pesquisas são realizadas com o objetivo de identificar e caracterizar os isolados, bem como desenvolver e aprimorar as técnicas para a identificação do gênero, permitindo a determinação da prevalência deste patógeno nas principais fontes de veiculação, como a carne de frango. O presente estudo teve como objetivo verificar a ocorrência de Campylobacter spp. e identificar as espécies termofílicas isoladas de carcaças de frango de abatedouros do estado de Goiás submetidos ao Serviço de Inspeção Federal e que possuíam autorização para o comércio interno e externo. Um total de 200 amostras foi avaliado utilizando o ensaio imunoenzimático como triagem, com posterior isolamento bacteriano e identificação por meio da biotipificação. Observou-se que os métodos empregados permitiram a identificação de Campylobacter spp. em 24% (48/200) das amostras avaliadas. Destas, 21 (10,5%) foram comprovadamente positivas para o gênero, tendo por fundamentação o isolamento bacteriano de amostras positivas à triagem e testes de assimilação enzimática, bioquímicas e de perfil antimicrobiano. Campylobacter coli foi a espécie mais isolada (58,6%), seguida por Campylobacter jejuni ssp. jejuni (17,2%), Campylobacter lari (13,8%) e Campylobacter jejuni ssp. doylei (3,4%). Observou-se que alguns isolados sugestivos, por apresentar positividade no teste de triagem, crescimento em meios seletivos, temperatura de incubação, morfologia e motilidade características do gênero em questão, não foram identificados pelo sistema numérico empregado neste estudo e, portanto, foram considerados negativos. Pelos resultados, pode-se concluir que a associação do método de triagem ao isolamento convencional e perfil numérico para biotipificação permitiram melhor identificação dos isolados. Assim, é possível afirmar que Campylobacter spp. foi identificada em carne de frango destinada ao consumo, havendo a descrição de três espécies termofílicas e duas subespécies. A presença deste patógeno confere risco à saúde pública por ingestão de alimentos contaminados e decorrência de doenças gastroentéricas e extraintestinais e em casos mais graves, porém não raros, a síndromes reumatológicas, oftalmológicas e neurológicas. Especificamente as espécies Campylobacter coli e Campylobacter jejuni descritas como de maior interesse em alimentos foram as de maior frequência e relacionam-se à maioria dos casos de campilobacteriose de origem alimentos.

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Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq
Thermophilic species of Campylobacter spp. are often cited as the most common etiologic agent in cases of foodborne illnesses in several countries. When ingested even in small quantities (400 to 500 cells) this organism, through the mechanisms of pathogenicity, the host develops infectious gastroenteritis known as campylobacteriosis. This disease is closely related to the intake and improper handling of contaminated food, especially poultry, and birds the main reservoir of the microorganism. Poor knowledge climbs the role of eggs in the transmission of campylobacteriosis, based on the annual consumption of the food matrix, research clarifying all variables involving this food are essential. This study aimed to verify the occurrence of Campylobacter spp., identify thermophilic species involved and assess the level of antimicrobial susceptibility of isolates from chicken carcasses and eggs obtained from commercial establishments in the city of Goiânia. A total of 100 chicken carcasses and 100 dozens of eggs were evaluated using enzyme- linked immunosorbent assay for screening miniVIDAS ® with subsequent bacterial isolation and identification of samples by biotyping . By means of the methods employed observed that Campylobacter spp. was identified in 7% (7 /100) of chicken carcasses in 1% (1/100 ) of samples of eggs, 100 % of these were confirmed as belonging to the bacterial genus . The species with the highest number of isolates were Campylobacter jejuni (50 %), followed by Campylobacter coli ( 25 % ) and Campylobacter lari ( 25 % ). For antimicrobial resistance results were that 100% of the isolates were resistant to erythromycin, as well as ciprofloxacin and tetracyclines. For ceftriaxone and nalidixic acid resistance values were 91.7 % resistant. Chloramphenicol was 50% of resistant isolates, showed 33.3% sensitivity and 16.7% intermediate resistance. It was also observed that 100% of the isolates showed multidrug profile. Through the results obtained it is concluded that thermophilic Campylobacter are present in chicken carcasses and marketed in Goiânia eggs, whose distribution becomes relevant from the point of view of exposure to consumers. Campylobacter jejuni was the most frequent species. The six districts surveyed had bacteria of the genus in at least one category. Isolates when screened for response to antimicrobial proved multiresistant, denoting the importance of research as a way to support the election of bases and principles for the antibiotic treatment prescribed treatments. Poultry foods surveyed in this study and considered positive for the pathogen constitute risk to the consumer.
As espécies termotolerantes de Campylobacter spp. são frequentemente apontadas como o agente etiológico mais comum nos casos de doenças veiculadas por alimentos em diversos países. Ao ser ingerido mesmo em pequenas quantidades (de 400 a 500 células), esse microrganismo, por meio dos mecanismos de patogenicidade, desenvolve gastroenterite infecciosa no hospedeiro, conhecida como campilobacteriose. Esta doença está intimamente relacionada à ingestão e manipulação incorreta de alimentos contaminados, especialmente carne de frango, sendo as aves o principal reservatório. Pouco se sabe sobre o papel desempenhado pelos ovos na transmissão de campilobacteriose, tendo como base o consumo anual dessa matriz alimentar, pesquisas que esclareçam todas as variáveis envolvendo esse alimento são de fundamental importância. Pelo exposto, este estudo teve como objetivo verificar a ocorrência de Campylobacter spp., identificar as espécies termotolerantes circulantes na cidade de Goiânia, vinculadas aos estabelecimentos comercializadores de alimentos, relacionados à alta densidade populacional, localizados em distritos comerciais e avaliar o nível de suscetibilidade a antimicrobianos dos isolados de carcaças de frango e ovos provenientes destes estabelecimentos. Um total de 100 carcaças de frango e 100 dúzias de ovos foram avaliados utilizando como triagem o ensaio imunoenzimatico miniVIDAS® com posterior isolamento bacteriano das amostras e identificação por meio da biotipificação. Por meio dos métodos empregados observou-se que Campylobacter termotolerantes foi identificada em 7% (7/100) das carcaças de frango e em 1% (1/100) das amostras de ovos. A espécie com maior número de isolados foi Campylobacter jejuni (50%), seguidos por Campylobacter coli (25%) e Campylobacter lari (25%). Para a resistência à antimicrobianos os resultados reportaram que 100% dos isolados são resistentes à eritromicina, assim como à ciprofloxacina e tetraciclina. Para ácido nalidixico e ceftriaxona, os valores de resistência foram da ordem de 91,7%. Cloranfenicol foi resistente em 50% dos isolados, apresentou sensibilidade a 33,3% e resistência intermediária a 16,7%. Observou-se ainda que 100% dos isolados apresentaram perfil de multirresistência. Pelos resultados obtidos conclui-se que Campylobacter termotolerantes estão presentes em carcaças de frango e ovos comercializados na cidade de Goiânia, cuja distribuição torna-se relevante do ponto de vista da exposição aos consumidores. Campylobacter jejuni foi a espécie mais frequente. Os seis distritos pesquisados apresentaram bactérias do gênero pelo menos em um estabelecimento. Os isolados quando pesquisados quanto a resposta a antimicrobianos mostraram-se multirresistentes, denotando a importância desta investigação como forma de respaldar a eleição de bases e princípios para a antibioticoterapia para tratamentos prescritos. Os alimentos avícolas pesquisados neste estudo e considerados positivos para o patógeno constituem risco ao consumidor.