Academic literature on the topic 'CaMV35S promoter'

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Journal articles on the topic "CaMV35S promoter"

1

Upadhyaya, Narayana M., Brian Surin, Kerrie Ramm, et al. "Agrobacterium-mediated transformation of Australian rice cultivars Jarrah and Amaroo using modified promoters and selectable markers." Functional Plant Biology 27, no. 3 (2000): 201. http://dx.doi.org/10.1071/pp99078.

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We report the first successfulAgrobacterium-mediated transformation of Australian elite rice cultivars, Jarrah and Amaroo, using binary vectors with our improved promoters and selectable markers. Calli derived from mature embryos were used as target tissues. The binary vectors contained hph(encoding hygromycin resistance) or bar (encoding herbicide resistance) as the selectable marker gene and uidA (gus) or sgfpS65T as the reporter gene driven by different promoters. Use of Agrobacterium strain AGL1 carrying derivatives of an improved binary vector pWBVec8, wherein the CaMV35S driven hph gene
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2

Ashraf, Muhammad Aleem, Ahmad Ali Shahid, Abdul Qayyum Rao, Judith K. Brown, and Tayyab Husnain. "Development and Evaluation of the Cotton Leaf Curl Kokhran Virus-Burewala Bidirectional Promoter for Enhanced Cry1Ac Endotoxin Expression in Bt Transgenic Cotton." Applied Sciences 12, no. 21 (2022): 11275. http://dx.doi.org/10.3390/app122111275.

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Fluctuation in Cry1Ac endotoxin levels expressed in transgenic Bacillus thuringiensis (Bt) cotton (Gossypium hirsutum L.) can result in a variation in efficacy throughout the growing season. Here, a green tissue-specific strong promoter of the cotton leaf curl Kokhran virus-Burewala (CLCuKoV-Bu) C1 gene is reported that can direct consistently high levels of Cry1Ac endotoxin expression in transformed cotton plants. The objective of this study was to investigate the capacities of the CLCuKoV-BuC1 promoter to drive transcription of Cry1Ac and stably express endotoxin in mature leaves and bolls o
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3

Xin, Shichao, Jinu Udayabhanu, Xuemei Dai, et al. "Identification and Functional Evaluation of Three Polyubiquitin Promoters from Hevea brasiliensis." Forests 13, no. 6 (2022): 952. http://dx.doi.org/10.3390/f13060952.

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Hevea brasiliensis is an economically important tree species that provides the only commercial source of natural rubber. The replacement of the CaMV35S promoter by endogenous polyubiquitin promoters may be a viable way to improve the genetic transformation of this species. However, no endogenous polyubiquitin promoters in Hevea have been reported yet. Here, we identified three Hevea polyubiquitin genes HbUBI10.1, HbUBI10.2 and HbUBI10.3, which encode ubiquitin monomers having nearly identical amino acid sequences to that of AtUBQ10. The genomic fragments upstream of these HbUBI genes, includin
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4

Martin, D. P., and E. P. Rybicki. "Improved Efficiency of Zea mays Agroinoculation with Maize streak virus." Plant Disease 84, no. 10 (2000): 1096–98. http://dx.doi.org/10.1094/pdis.2000.84.10.1096.

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Agroinoculation is a technique permitting the transmission of geminivirus genomes cloned in Agrobacterium tumefaciens into a wide variety of mono- and dicotyledonous host plants. Most geminiviruses are obligately transmitted by insect vector species under natural conditions; therefore, agroinoculation has greatly simplified the study of this group of viruses. In many cases, agroinoculation has replaced insect transmission, and has been used to compare virulence characteristics among viruses. Here we report on the discovery that, in agroinfectious Maize streak virus constructs, the orientation
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5

Dai, Wenhao, Zong-Ming Cheng та Wayne Sargent. "486 Expression of β-glucuronidase Gene in Aspen under Control of CaMV35S, Heat Shock and RolC Promoters". HortScience 35, № 3 (2000): 478A—478. http://dx.doi.org/10.21273/hortsci.35.3.478a.

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Transgenic hybrid aspens (Populus tremuloides × P. tremula) were produced by Agrobacterium-mediated transformation and confirmed by polymerase chain reaction. Three promoters (CaMV 35S, Heat shock, and Rol C) were used to drive transcription of chimeric genes -glucuronidase (GUS), npt-II, and rol B. Stem sections in ≈100 mm thick, leaf blades, and root tips of transgenic aspen were treated with X-Gluc solution for 2 to 12 h in a 37 °C incubator and fixed in a solution containing 5% formaldehyde, 5% acetic acid, and 20% ethanol (FAA) for 10 min. After washing with 50% ethanol twice and clearing
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6

Naveenarani, Murugan, Huskur Kumaraswamy Mahadeva Swamy, Sakthivel Surya Krishna, et al. "Isolation and Characterization of Erianthus arundinaceus Phosphate Transporter 1 (PHT1) Gene Promoter and 5′ Deletion Analysis of Transcriptional Regulation Regions under Phosphate Stress in Transgenic Tobacco." Plants 12, no. 21 (2023): 3760. http://dx.doi.org/10.3390/plants12213760.

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Phosphorus deficiency highly interferes with plant growth and development. Plants respond to persistent P deficiency by coordinating the expression of genes involved in the alleviation of stress. Promoters of phosphate transporter genes are a great choice for the development of genetically modified plants with enhanced phosphate uptake abilities, which improve crop yields in phosphate-deficient soils. In our previous study, the sugarcane phosphate transporter PHT1;2 gene showed a significantly high expression under salinity stress. In this study, the Erianthus arundinaceus EaPHT1;2 gene was is
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7

Yi, Jung-Yoon, Hyo-Won Seo, Kwan-Soo Kim, Hyun-Mook Cho, and Kuen-Woo Park. "068 Development of the Bacterial Disease Resistant Potatoes by Introduction of Shiva Gene." HortScience 35, no. 3 (2000): 400C—400. http://dx.doi.org/10.21273/hortsci.35.3.400c.

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Antimicrobial peptide gene (shiva) under the promoter of tomato phenylalanine ammonia-lyase (tPAL5) was transformed into potato (Solanum tuberosum L.) plants. Antimicrobial peptide gene was isolated originally from giant silk moth (Hyalophora cecropia) and modified its nucleotide seqnence to increase antimicrobial activity. Phenylalanine ammonia lyase 5 (PAL5) gene was known to express highly by wounding, irradiation, and infection by pathogens. It also expresses specifically on vessel tissues of young roots, stems, and leaves. The vector with shiva and CaMV35S promoter was also introduced int
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8

Maekawa, Takaki, Mitsumasa Kusakabe, Yoshikazu Shimoda, et al. "Polyubiquitin Promoter-Based Binary Vectors for Overexpression and Gene Silencing in Lotus japonicus." Molecular Plant-Microbe Interactions® 21, no. 4 (2008): 375–82. http://dx.doi.org/10.1094/mpmi-21-4-0375.

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In this study, we compared the transcriptional activities between Cauliflower mosaic virus (CaMV)35S promoter and polyubiquitin (Ljubq1) promoter from Lotus japonicus using β-glucuronidase (gus) reporter gene in transgenic plants of L. japonicus. The promoter analysis demonstrated that the Ljubq1 promoter possessed higher activity than the CaMV35S promoter in leaves, stems, roots, nodules, and pollen. Finally, we created GATEWAY conversion technology-compatible binary vectors for over-expression and RNA interference under the Ljubq1 promoter. These materials could provide alternative choice fo
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9

Rehana, S. Baishakh N. Datta K. Oliva N. Abrigo E. Mazid M.A. Shah-E.-Alam Uddin M.R. and Datta S.K. "Engineering provitamin A synthesis pathway with β-carotene metabolism in rice endosperm of a restorer line BR827R". Archives of Agriculture and Environmental Science 3, № 2 (2018): 103–8. https://doi.org/10.26832/24566632.2018.030201.

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Vitamin A deficiency (VAD) is a serious public health problem in South Asia particularly in Bangladesh. Indica rice as a major staple in the country completely lacks vitamin A or compounds with provitamin A activity after milling. A combination of transgenes has been introduced enabling biosynthesis of provitamin A in the endosperm of a restorer line using biolistic system of transformation. The rice seed-specific glutelin promoter (Gt-1 P) was used to drive the expression of phytone synthase (psy), while lycopene b-cyclase (lcy) and phytoenedesaturase (crtI), fused to the transit peptide sequ
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10

López, Marian, Jaime M. Humara, Roberto Rodríguez, and Ricardo J. Ordás. "Transient uidA gene expression in electroporated cotyledonary protoplasts of Pinus nigra ssp. salzmannii and in bombarded cotyledons." Canadian Journal of Forest Research 30, no. 3 (2000): 448–55. http://dx.doi.org/10.1139/x99-228.

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We have developed a method for the routine isolation of Pinus nigra Arn. ssp. salzmannii (Dunal) Franco protoplasts. The optimized electroporation conditions for uidA gene (gene for beta-glucuronidase) expression in protoplasts from cotyledons excised from 8-day-old seedlings were determined using an exponential pulse wave generator for gene transfer. The protoplasts were electroporated with plasmids containing the chimeric uidA gene under the control of several promoters, and in parallel, cotyledons were bombarded with the same constructs using a biolistic gun. Both techniques confirmed that
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