Log in

Relevant bibliographies by topics / CaMV35S promoter / Journal articles

To see the other types of publications on this topic, follow the link: CaMV35S promoter.

Journal articles on the topic 'CaMV35S promoter'

Author: Grafiati

Published: 5 June 2025

Last updated: 1 August 2025

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'CaMV35S promoter.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Upadhyaya, Narayana M., Brian Surin, Kerrie Ramm, et al. "Agrobacterium-mediated transformation of Australian rice cultivars Jarrah and Amaroo using modified promoters and selectable markers." Functional Plant Biology 27, no. 3 (2000): 201. http://dx.doi.org/10.1071/pp99078.

Full text

Abstract:

We report the first successfulAgrobacterium-mediated transformation of Australian elite rice cultivars, Jarrah and Amaroo, using binary vectors with our improved promoters and selectable markers. Calli derived from mature embryos were used as target tissues. The binary vectors contained hph(encoding hygromycin resistance) or bar (encoding herbicide resistance) as the selectable marker gene and uidA (gus) or sgfpS65T as the reporter gene driven by different promoters. Use of Agrobacterium strain AGL1 carrying derivatives of an improved binary vector pWBVec8, wherein the CaMV35S driven hph gene

APA, Harvard, Vancouver, ISO, and other styles

2

Ashraf, Muhammad Aleem, Ahmad Ali Shahid, Abdul Qayyum Rao, Judith K. Brown, and Tayyab Husnain. "Development and Evaluation of the Cotton Leaf Curl Kokhran Virus-Burewala Bidirectional Promoter for Enhanced Cry1Ac Endotoxin Expression in Bt Transgenic Cotton." Applied Sciences 12, no. 21 (2022): 11275. http://dx.doi.org/10.3390/app122111275.

Full text

Abstract:

Fluctuation in Cry1Ac endotoxin levels expressed in transgenic Bacillus thuringiensis (Bt) cotton (Gossypium hirsutum L.) can result in a variation in efficacy throughout the growing season. Here, a green tissue-specific strong promoter of the cotton leaf curl Kokhran virus-Burewala (CLCuKoV-Bu) C1 gene is reported that can direct consistently high levels of Cry1Ac endotoxin expression in transformed cotton plants. The objective of this study was to investigate the capacities of the CLCuKoV-BuC1 promoter to drive transcription of Cry1Ac and stably express endotoxin in mature leaves and bolls o

APA, Harvard, Vancouver, ISO, and other styles

3

Xin, Shichao, Jinu Udayabhanu, Xuemei Dai, et al. "Identification and Functional Evaluation of Three Polyubiquitin Promoters from Hevea brasiliensis." Forests 13, no. 6 (2022): 952. http://dx.doi.org/10.3390/f13060952.

Full text

Abstract:

Hevea brasiliensis is an economically important tree species that provides the only commercial source of natural rubber. The replacement of the CaMV35S promoter by endogenous polyubiquitin promoters may be a viable way to improve the genetic transformation of this species. However, no endogenous polyubiquitin promoters in Hevea have been reported yet. Here, we identified three Hevea polyubiquitin genes HbUBI10.1, HbUBI10.2 and HbUBI10.3, which encode ubiquitin monomers having nearly identical amino acid sequences to that of AtUBQ10. The genomic fragments upstream of these HbUBI genes, includin

APA, Harvard, Vancouver, ISO, and other styles

4

Martin, D. P., and E. P. Rybicki. "Improved Efficiency of Zea mays Agroinoculation with Maize streak virus." Plant Disease 84, no. 10 (2000): 1096–98. http://dx.doi.org/10.1094/pdis.2000.84.10.1096.

Full text

Abstract:

Agroinoculation is a technique permitting the transmission of geminivirus genomes cloned in Agrobacterium tumefaciens into a wide variety of mono- and dicotyledonous host plants. Most geminiviruses are obligately transmitted by insect vector species under natural conditions; therefore, agroinoculation has greatly simplified the study of this group of viruses. In many cases, agroinoculation has replaced insect transmission, and has been used to compare virulence characteristics among viruses. Here we report on the discovery that, in agroinfectious Maize streak virus constructs, the orientation

APA, Harvard, Vancouver, ISO, and other styles

5

Dai, Wenhao, Zong-Ming Cheng та Wayne Sargent. "486 Expression of β-glucuronidase Gene in Aspen under Control of CaMV35S, Heat Shock and RolC Promoters". HortScience 35, № 3 (2000): 478A—478. http://dx.doi.org/10.21273/hortsci.35.3.478a.

Full text

Abstract:

Transgenic hybrid aspens (Populus tremuloides × P. tremula) were produced by Agrobacterium-mediated transformation and confirmed by polymerase chain reaction. Three promoters (CaMV 35S, Heat shock, and Rol C) were used to drive transcription of chimeric genes -glucuronidase (GUS), npt-II, and rol B. Stem sections in ≈100 mm thick, leaf blades, and root tips of transgenic aspen were treated with X-Gluc solution for 2 to 12 h in a 37 °C incubator and fixed in a solution containing 5% formaldehyde, 5% acetic acid, and 20% ethanol (FAA) for 10 min. After washing with 50% ethanol twice and clearing

APA, Harvard, Vancouver, ISO, and other styles

6

Naveenarani, Murugan, Huskur Kumaraswamy Mahadeva Swamy, Sakthivel Surya Krishna, et al. "Isolation and Characterization of Erianthus arundinaceus Phosphate Transporter 1 (PHT1) Gene Promoter and 5′ Deletion Analysis of Transcriptional Regulation Regions under Phosphate Stress in Transgenic Tobacco." Plants 12, no. 21 (2023): 3760. http://dx.doi.org/10.3390/plants12213760.

Full text

Abstract:

Phosphorus deficiency highly interferes with plant growth and development. Plants respond to persistent P deficiency by coordinating the expression of genes involved in the alleviation of stress. Promoters of phosphate transporter genes are a great choice for the development of genetically modified plants with enhanced phosphate uptake abilities, which improve crop yields in phosphate-deficient soils. In our previous study, the sugarcane phosphate transporter PHT1;2 gene showed a significantly high expression under salinity stress. In this study, the Erianthus arundinaceus EaPHT1;2 gene was is

APA, Harvard, Vancouver, ISO, and other styles

7

Yi, Jung-Yoon, Hyo-Won Seo, Kwan-Soo Kim, Hyun-Mook Cho, and Kuen-Woo Park. "068 Development of the Bacterial Disease Resistant Potatoes by Introduction of Shiva Gene." HortScience 35, no. 3 (2000): 400C—400. http://dx.doi.org/10.21273/hortsci.35.3.400c.

Full text

Abstract:

Antimicrobial peptide gene (shiva) under the promoter of tomato phenylalanine ammonia-lyase (tPAL5) was transformed into potato (Solanum tuberosum L.) plants. Antimicrobial peptide gene was isolated originally from giant silk moth (Hyalophora cecropia) and modified its nucleotide seqnence to increase antimicrobial activity. Phenylalanine ammonia lyase 5 (PAL5) gene was known to express highly by wounding, irradiation, and infection by pathogens. It also expresses specifically on vessel tissues of young roots, stems, and leaves. The vector with shiva and CaMV35S promoter was also introduced int

APA, Harvard, Vancouver, ISO, and other styles

8

Maekawa, Takaki, Mitsumasa Kusakabe, Yoshikazu Shimoda, et al. "Polyubiquitin Promoter-Based Binary Vectors for Overexpression and Gene Silencing in Lotus japonicus." Molecular Plant-Microbe Interactions® 21, no. 4 (2008): 375–82. http://dx.doi.org/10.1094/mpmi-21-4-0375.

Full text

Abstract:

In this study, we compared the transcriptional activities between Cauliflower mosaic virus (CaMV)35S promoter and polyubiquitin (Ljubq1) promoter from Lotus japonicus using β-glucuronidase (gus) reporter gene in transgenic plants of L. japonicus. The promoter analysis demonstrated that the Ljubq1 promoter possessed higher activity than the CaMV35S promoter in leaves, stems, roots, nodules, and pollen. Finally, we created GATEWAY conversion technology-compatible binary vectors for over-expression and RNA interference under the Ljubq1 promoter. These materials could provide alternative choice fo

APA, Harvard, Vancouver, ISO, and other styles

9

Rehana, S. Baishakh N. Datta K. Oliva N. Abrigo E. Mazid M.A. Shah-E.-Alam Uddin M.R. and Datta S.K. "Engineering provitamin A synthesis pathway with β-carotene metabolism in rice endosperm of a restorer line BR827R". Archives of Agriculture and Environmental Science 3, № 2 (2018): 103–8. https://doi.org/10.26832/24566632.2018.030201.

Full text

Abstract:

Vitamin A deficiency (VAD) is a serious public health problem in South Asia particularly in Bangladesh. Indica rice as a major staple in the country completely lacks vitamin A or compounds with provitamin A activity after milling. A combination of transgenes has been introduced enabling biosynthesis of provitamin A in the endosperm of a restorer line using biolistic system of transformation. The rice seed-specific glutelin promoter (Gt-1 P) was used to drive the expression of phytone synthase (psy), while lycopene b-cyclase (lcy) and phytoenedesaturase (crtI), fused to the transit peptide sequ

APA, Harvard, Vancouver, ISO, and other styles

10

López, Marian, Jaime M. Humara, Roberto Rodríguez, and Ricardo J. Ordás. "Transient uidA gene expression in electroporated cotyledonary protoplasts of Pinus nigra ssp. salzmannii and in bombarded cotyledons." Canadian Journal of Forest Research 30, no. 3 (2000): 448–55. http://dx.doi.org/10.1139/x99-228.

Full text

Abstract:

We have developed a method for the routine isolation of Pinus nigra Arn. ssp. salzmannii (Dunal) Franco protoplasts. The optimized electroporation conditions for uidA gene (gene for beta-glucuronidase) expression in protoplasts from cotyledons excised from 8-day-old seedlings were determined using an exponential pulse wave generator for gene transfer. The protoplasts were electroporated with plasmids containing the chimeric uidA gene under the control of several promoters, and in parallel, cotyledons were bombarded with the same constructs using a biolistic gun. Both techniques confirmed that

APA, Harvard, Vancouver, ISO, and other styles

11

Sargent, Wayne A., and Zong-Ming Cheng. "Expression of “Rooting” Genes iaaM and rolB Under Regulatory Promoters in Tobacco." HortScience 32, no. 3 (1997): 481E—482. http://dx.doi.org/10.21273/hortsci.32.3.481e.

Full text

Abstract:

Hybrid aspen is a major source of fiber in the north-central United States. One major problem for utilizing genetically improved aspen, and a variety of other woody species, is the difficulty in rooting hardwood cuttings. The objective of this project was to construct and confirm the function of genetic transformation vectors with two rooting genes (iaaM from Agrobacterium tumefaciens and rolB from A. rhizogenes) using three promoters (CaMV35S, soybean heat shock-inducible and poplar wound-inducible promoters). The gene constructs can be used to transform aspen for improvement of rooting hardw

APA, Harvard, Vancouver, ISO, and other styles

12

Park, Soo Min, and Choo Bong Hong. "Transcription of human lnterleukin-2 gene inNicotiana tabacum driven by CaMV35S promoter." Journal of Plant Biology 40, no. 2 (1997): 110–14. http://dx.doi.org/10.1007/bf03030242.

Full text

APA, Harvard, Vancouver, ISO, and other styles

13

Guo, Qi, Nan Lu, Yuhan Sun, et al. "Heterologous Expression of the DREB Transcription Factor AhDREB in Populus tomentosa Carrière Confers Tolerance to Salt without Growth Reduction under Greenhouse Conditions." Forests 10, no. 3 (2019): 214. http://dx.doi.org/10.3390/f10030214.

Full text

Abstract:

The DREB transcription factors regulate multiple stress response genes, and are therefore useful for molecular plant breeding. AhDREB, a stress-inducible gene, was isolated from Atriplex hortensis L. and introduced into Populus tomentosa Carrière under the control of the CaMV35S promoter. Under salt stress, the chlorophyll content and net photosynthetic rate were higher in transgenic lines than in the wild type (WT). Moreover, the rate of electrolyte penetration (REC) was lower in the transgenic lines. Additional analyses revealed that the AhDREB transgenic plants generally displayed lower mal

APA, Harvard, Vancouver, ISO, and other styles

14

Pang, Yue-Hong, Yi-Ying Wang, Meng-Meng Sun, and Xiao-Fang Shen. "Visual detection of CaMV35S promoter via target-triggered rolling circle amplification of DNAzyme." Journal of Food Composition and Analysis 106 (March 2022): 104304. http://dx.doi.org/10.1016/j.jfca.2021.104304.

Full text

APA, Harvard, Vancouver, ISO, and other styles

15

Setyati, Sri, Purnama Oktaviandari, Muhammad Hazmi, and Bambang Sugiharto. "STUDI PERBANDINGAN METODE TRANSFORMASI DNA MENGGUNAKAN VEKTOR Agrobacterium tumefaciens PADA TANAMAN TEBU (Sacharum hybrid)." Berkala Penelitian Hayati 13, no. 1 (2007): 39–44. http://dx.doi.org/10.23869/bphjbr.13.1.20076.

Full text

Abstract:

In order to compare transient expression of gus gene driven by CaMV 35S and rice ubiquitin RUBQ2 promoters, a DNA transformation was conducted using embryogenic callus and suspension cultures of sugarcane. The transient gus expression was observed by histochemical staining method. The histochemical observation of GUS activity after co-cultivation showed that RUBQ2 promoter produced high level of clear blue spots both in embryogenic callus and suspension cultures, while the CaMV35S promoter was not detected. The suspension cultures slightly increased transient gus gene expression compared to em

APA, Harvard, Vancouver, ISO, and other styles

16

Zhang, Chen-Yi, Zha-Long Ye, Li-Wang Qi, Ling Yang, and Wan-Feng Li. "Screening and Functional Evaluation of Four Larix kaempferi Promoters." Plants 13, no. 19 (2024): 2777. http://dx.doi.org/10.3390/plants13192777.

Full text

Abstract:

Promoters are powerful tools for breeding new varieties using transgenic technology. However, the low and unstable expression of target genes is still a limiting factor in Larix kaempferi (Lamb.) Carr (Japanese larch) genetic transformation. In this study, we analyzed L. kaempferi transcriptome data, screened out highly expressed genes, cloned their promoters, and constructed plant expression vectors containing the β-glucuronidase (GUS) reporter gene driven by these promoters. Recombinant vectors were introduced into the L. kaempferi embryogenic callus by means of the Agrobacterium-mediated tr

APA, Harvard, Vancouver, ISO, and other styles

17

Barotov, Samariddin S., Firuza Yu Nasyrova, and Farzona A. Abdukholiqova. "Identification of genetically modified crops in Tajikistan." Ecological genetics 21, no. 3S (2023): 75–76. http://dx.doi.org/10.17816/ecogen568487.

Full text

Abstract:

The technology of genetically modified crops (also called GM crops, GM products) allows, using genetic engineering methods, to select a specific genetic trait of one organism for inclusion in the genome of the original crop. This has made it possible to develop food crops with beneficial properties and eliminate undesirable properties in others. Despite the great agricultural benefits of transgenic crops, they have not gained acceptance in some countries: a) consumer suspicion due to allergic reactions observed to some transgenic products, b) lack of international regulations regarding these G

APA, Harvard, Vancouver, ISO, and other styles

18

Amack, Stephanie C., and Mauricio S. Antunes. "CaMV35S promoter – A plant biology and biotechnology workhorse in the era of synthetic biology." Current Plant Biology 24 (December 2020): 100179. http://dx.doi.org/10.1016/j.cpb.2020.100179.

Full text

APA, Harvard, Vancouver, ISO, and other styles

19

Li, Baoxia, Liuqin Zhang, Jing Xi, et al. "An Unexpected Regulatory Sequence from Rho-Related GTPase6 Confers Fiber-Specific Expression in Upland Cotton." International Journal of Molecular Sciences 23, no. 3 (2022): 1087. http://dx.doi.org/10.3390/ijms23031087.

Full text

Abstract:

Cotton fibers, single seed trichomes derived from ovule epidermal cells, are the major source of global textile fibers. Fiber-specific promoters are desirable to study gene function and to modify fiber properties during fiber development. Here, we revealed that Rho-related GTPase6 (GhROP6) was expressed preferentially in developing fibers. A 1240 bp regulatory region of GhROP6, which contains a short upstream regulatory sequence, the first exon, and the partial first intron, was unexpectedly isolated and introduced into transgenic cotton for analyzing promoter activity. The promoter of GhROP6

APA, Harvard, Vancouver, ISO, and other styles

20

Song, Xiang Li, Zhi Hui Wu, Xi Zhang, Meng Xi Lu, and Bei Guo. "Construction of Expression Vector with Fruit-Specific Promoter and Genetic Transformation of Strawberry." Advanced Materials Research 690-693 (May 2013): 1314–17. http://dx.doi.org/10.4028/www.scientific.net/amr.690-693.1314.

Full text

Abstract:

In order to transcription and expression an exogenous gene just in transgenic strawberry fruit, the gene encoding tomato fruit-specific E8 promoter was cloned. We constructed plant expression vector with E8 promoter replace the CaMV35S of pBI121 that used for transformation of strawberry. Recombination plasmid was identified by PCR, restriction enzymes digestion and sequencing analysis, and transfered into the agrobacterium EHA105 strain. At the same time, the agrobacterium EHA105, transformation and no transformation tissue of strawberry used as the experiment materials to study the expressio

APA, Harvard, Vancouver, ISO, and other styles

21

Kirienko, Anna N., and Elena A. Dolgikh. "Studying the effect of tissue-specific expression of the K1 gene encoding LysM-receptor-like kinase on the development of symbiosis in peas." BIO Web of Conferences 23 (2020): 03005. http://dx.doi.org/10.1051/bioconf/20202303005.

Full text

Abstract:

To study the role of pea LysM receptor-like kinase K1 in the coordination of the infection process, starting in epidermis and nodule organogenesis in the root cortex of plants, during the development of rhizobium-legume symbiosis, the genetic constructs in which K1 gene was cloned under the control of tissue-specific promoter pLeEXT1 of tomato Lycopersicon esculentum extensin gene and the constitutive promoter of cauliflower mosaic virus (CaMV35S, cauliflower mosaic virus 35S) were obtained. During the transformation of the Nod- mutant line, the k1-1, with two types of constructs, the restorat

APA, Harvard, Vancouver, ISO, and other styles

22

Wang, Qingxiang, Jiancong Ni, Feng Gao, Bin Zhang, Shunxing Li, and Kui Jiao. "Use of a chitosan–cadmium polymer as a redox hybridization indicator for CaMV35S promoter gene detection." Journal of Bioactive and Compatible Polymers 27, no. 3 (2012): 278–92. http://dx.doi.org/10.1177/0883911512440537.

Full text

APA, Harvard, Vancouver, ISO, and other styles

23

Galovic, Vladislava, T. Rausch, and Slobodanka Grsic-Rausch. "Mature embryo-derived wheat transformation with major stress-modulated antioxidant target gene." Archives of Biological Sciences 62, no. 3 (2010): 539–46. http://dx.doi.org/10.2298/abs1003539g.

Full text

Abstract:

The mature embryos of fourteen elite winter wheat cultivars have been transformed by a biolistic approach. The gene coding for ?-glutamylcysteine synthetase (EC 6.3.2.2) was used as a transgene in order to obtain stable transformants resistant to drought stress. A binary vector, pBinarUTRECS, was used. The gene was under the control of the CaMV35S promoter region. GUS::GFP gene fusion was used as a reporter system and nptII served as a selectable marker gene. A high regeneration capacity of callus tissue under the selective pressure and successful GUS assay of transformed tissue were an indica

APA, Harvard, Vancouver, ISO, and other styles

24

Strelnikova, Svetlana R., Anastasiya A. Krinitsina, and Roman A. Komakhin. "Effective RNAi-Mediated Silencing of the Mismatch Repair MSH2 Gene Induces Sterility of Tomato Plants but Not an Increase in Meiotic Recombination." Genes 12, no. 8 (2021): 1167. http://dx.doi.org/10.3390/genes12081167.

Full text

Abstract:

In plant breeding, the ability to manipulate meiotic recombination aids in the efficient construction of new allelic compositions of chromosomes and facilitates gene transfer from wild relatives of crop plants. The DNA mismatch repair system antagonizes meiotic recombination. In this research, a trial was conducted to evaluate transgenic tomato plants carrying an RNA interference (RNAi) construct designed to inhibit the expression of the mismatch repair MSH2 gene. To drive the RNAi construct, we used either a pro-SmAMP2 promoter from Stellaria media ANTIMICROBIAL PEPTIDE2 or a Cauliflower mosa

APA, Harvard, Vancouver, ISO, and other styles

25

Enomoto, Takuo, Mutsutomo Tokizawa, Hiroki Ito, et al. "STOP1 regulates the expression of HsfA2 and GDHs that are critical for low-oxygen tolerance in Arabidopsis." Journal of Experimental Botany 70, no. 12 (2019): 3297–311. http://dx.doi.org/10.1093/jxb/erz124.

Full text

Abstract:

Abstract The SENSITIVE TO PROTON RHIZOTOXICITY 1 (STOP1) transcription factor regulates gene expression associated with multiple stress tolerances in plant roots. In this study, we investigated the mechanism responsible for the sensitivity of the stop1 mutant to low-oxygen stress in Arabidopsis. Transcriptomic analyses revealed that two genes involved in low-oxygen tolerance, namely GLUTAMATE DEHYDROGENASE 1 (GDH1) and GDH2, showed lower expression levels in the stop1 mutant than in the wild-type. Sensitivity of the gdh1gdh2 double-mutant to low-oxygen conditions was partly attributable to the

APA, Harvard, Vancouver, ISO, and other styles

26

OGUCHI, Taichi, Mari ONISHI, Yukie CHIKAGAWA, et al. "Development of Event-Specific Quantitation Method for GA21 Maize, Which Is a GM Event without CaMV35S Promoter." Journal of the Food Hygienic Society of Japan (Shokuhin Eiseigaku Zasshi) 49, no. 1 (2008): 16–22. http://dx.doi.org/10.3358/shokueishi.49.16.

Full text

APA, Harvard, Vancouver, ISO, and other styles

27

Andalusia, Galih Gibral, Sony Suhandono, and Ima Mulyama Zainuddin. "Analysis of MeEf1A6 Gene Promoter Activity with In-vitro and In-vivo using Transient and Stable Expression Techniques in Tobacco Plant (Nicotiana tabacum)." 3BIO: Journal of Biological Science, Technology and Management 3, no. 1 (2021): 1–8. http://dx.doi.org/10.5614/3bio.2021.3.1.1.

Full text

Abstract:

The promoter is a part of the gene that functions in carrying out the gene expression, and its work activity becomes a matter of concern to ensure that expression works effectively. MeEF1A6 (Manihot esculenta Elongation Factor 1 Alfa - 6) is a promoter derived from cassava plants (Manihot esculenta). In previous studies, the MeEF1A6 promoter was successfully isolated, introduced, and characterized into the pBI121 plasmid, replacing the CaMV35S promoter. This study aims to analyze the activity of MeEF1A6 promoters in-vivo and in-vitro by using transient and transgenic techniques in tobacco plan

APA, Harvard, Vancouver, ISO, and other styles

28

Humara, Jaime M., M. Soledad Marín, Francisco Parra, and Ricardo J. Ordás. "Improved efficiency of uidA gene transfer in stone pine (Pinus pinea) cotyledons using a modified binary vector." Canadian Journal of Forest Research 29, no. 10 (1999): 1627–32. http://dx.doi.org/10.1139/x99-117.

Full text

Abstract:

This paper describes the construction of a binary vector with the uidA-int gene under the control of the promoter and first intron from the maize (Zea mays L.) ubiquitin 1 (Ubi1) gene, aiming at high expression levels of the uidA gene in Pinus pinea L. cotyledons. Transient expression was determined after microparticle bombardment of 1-day-old cotyledons, and data indicated that the new construct performed considerably better than p35SGUSint, which has the uidA-int gene under the control of a CaMV35S promoter. In addition, we have demonstrated that the marker gene uidA from plasmid pBINUbiGUSi

APA, Harvard, Vancouver, ISO, and other styles

29

de la Torre, Francisco, Ruth Rodríguez, Gago Jorge, et al. "Genetic transformation of Eucalyptus globulus using the vascular-specific EgCCR as an alternative to the constitutive CaMV35S promoter." Plant Cell, Tissue and Organ Culture (PCTOC) 117, no. 1 (2014): 77–84. http://dx.doi.org/10.1007/s11240-013-0422-z.

Full text

APA, Harvard, Vancouver, ISO, and other styles

30

Kianfar, Niloofar, and Maryam Heydarifard. "Rapid and accurate detection of genetically manipulated soybean using polymerase chain reaction." MOJ Current Research & Reviews 2, no. 2 (2019): 37–40. http://dx.doi.org/10.15406/mojcrr.2019.02.00053.

Full text

Abstract:

Background: It is controversy over the GM products consumption around the world. However, the limitations of food supplies and population growth have forced the posterity to use transgenic products. Accurate strategies for consumption and use of proper diagnostic methods are crucial in this regard. Materials and methods: The soybean commercial samples were purchased as a control from supermarkets located in Tehran. Considerable attention was paid to collecting samples from different brands. In this investigation, in order to identify the soybean oil in products, randomly, 25 raw oil and soybea

APA, Harvard, Vancouver, ISO, and other styles

31

Rajabi Memari, Hamid, Ramakrishnan Ramanan та Arbakariya Ariff. "Comparison of expression systems for the production of human interferon-α2b". Open Life Sciences 5, № 4 (2010): 446–55. http://dx.doi.org/10.2478/s11535-010-0036-y.

Full text

Abstract:

AbstractThe production of human interferon alpha2b (IFN-α2b) in two expression systems, tobacco (Nicotiana tabaccum) and Escherichia coli, was compared in various aspects such as safety, yield, quality of product and productivity. In the E. coli system, IFN-α2b was expressed under a pelB signal sequence and a T7lac promoter in a pET 26b(+) vector. The same gene was also cloned in expression plant vector (pCAMBIA1304) between cauliflower mosaic virus promoter (CaMV35S) and poly A termination region (Nos) and expressed in transgenic tobacco plants. The expression of protein in both systems was c

APA, Harvard, Vancouver, ISO, and other styles

32

Wang, Ya-Jie, Xiao-Hua Lu, Xing-Hou Zhen, et al. "A Transformation and Genome Editing System for Cassava Cultivar SC8." Genes 13, no. 9 (2022): 1650. http://dx.doi.org/10.3390/genes13091650.

Full text

Abstract:

Cassava starch is a widely used raw material for industrial production. South Chinese cassava cultivar 8 (Manihot esculenta Crantz cv. SC8) is one of the main locally planted cultivars. In this study, an efficient transformation system for cassava SC8 mediated with Agrobacterium strain LBA4404 was presented for the first time. Cassava friable embryogenic calli (FECs) were transformed through the binary vector pCAMBIA1304 harboring GUS- and GFP-fused genes driven by the CaMV35S promoter. The transformation efficiency was increased in the conditions of Agrobacterium strain cell infection density

APA, Harvard, Vancouver, ISO, and other styles

33

Pecchia, Paola, Maria Cammareri, Nicola Malafronte, M. Federica Consiglio, Maria Josefina Gualtieri та Clara Conicella. "Quinic Acids from Aster caucasicus and from Transgenic Callus Expressing a β-Amyrin Synthase§". Natural Product Communications 6, № 11 (2011): 1934578X1100601. http://dx.doi.org/10.1177/1934578x1100601125.

Full text

Abstract:

Several different classes of secondary metabolites, including flavonoids, triterpenoid saponins and quinic acid derivatives, are found in Aster spp. (Fam. Asteraceae). Several Aster compounds revealed biological as well as pharmacological activities. In this work, a phytochemical investigation of A. caucasicus evidenced the presence of quinic acid derivatives, as well as the absence of triterpene saponins. To combine in one species the production of different phytochemicals, including triterpenes, an Agrobacterium-mediated transformation of A. caucasicus was set up to introduce A. sedifolius β

APA, Harvard, Vancouver, ISO, and other styles

34

Wang, Xutong, Mandi Wang, Jian Sun, Xiaolei Qu, Shixin Wang, and Tingting Sun. "Establishment of an Efficient Genetic Transformation System in Sanghuangporus baumii." Journal of Fungi 10, no. 2 (2024): 137. http://dx.doi.org/10.3390/jof10020137.

Full text

Abstract:

(1) Background: Sanghuangporus baumii, a valuable medicinal fungus, has limited studies on its gene function due to the lack of a genetic transformation system. (2) Methods: This study aimed to establish an efficient Agrobacterium tumefaciens-mediated transformation (ATMT) system for S. baumii. This study involved cloning the promoter (glyceraldehyde-3-phosphate dehydrogenase, gpd) of S. baumii, reconstructing the transformation vector, optimizing the treatment of receptor tissues, and inventing a new method for screening positive transformants. (3) Results: The established ATMT system involve

APA, Harvard, Vancouver, ISO, and other styles

35

Majumder, Shuvobrata, Karabi Datta, Subrata Satpathy, and Swapan K. Datta. "Development and evaluation of lepidopteran insect resistant jute expressing the fused Bt-Cry1Ab/Ac toxin driven by CaMV35S promoter." Industrial Crops and Products 156 (November 2020): 112873. http://dx.doi.org/10.1016/j.indcrop.2020.112873.

Full text

APA, Harvard, Vancouver, ISO, and other styles

36

Chibbar, R. N., K. K. Kartha, N. Leung, J. Qureshi, and K. Caswell. "Transient expression of marker genes in immature zygotic embryos of spring wheat (Triticum aestivum) through microprojectile bombardment." Genome 34, no. 3 (1991): 453–60. http://dx.doi.org/10.1139/g91-068.

Full text

Abstract:

Transient expression of marker genes (cat and uidA) delivered by the Biolistics microprojectile bombardment technique has been detected in immature zygotic embryos of wheat (Triticum aestivum L.). The DNA expression vectors that gave maximal expression of both cat (pCaMVI1CN) and uidA (pCaMVI1GusN) genes had an alcohol dehydrogenase (Adh1) intron 1 cloned in between the cauliflower mosaic virus (CaMV35S) promoter and the coding region of the gene. Detection of chloramphenicol acetyltransferase (CAT) activity in response to cat gene was complicated by the presence of an inhibitor of CAT activit

APA, Harvard, Vancouver, ISO, and other styles

37

Zdjelar, G., Z. Nikolić, I. Vasiljević, et al. "Detection of genetically modified soya, maize, and rice in vegetarian and healthy food products in Serbia." Czech Journal of Food Sciences 31, No. 1 (2013): 43–48. http://dx.doi.org/10.17221/105/2012-cjfs.

Full text

Abstract:

The presence of genetic modifications was analysed in a total of 100 samples of non-labelled vegetarian and healthy food products. The basic raw materials in the samples tested comprised maize, soya, and/or rice. The screening of all samples was performed using the primers for CaMV35S promoter. The positive samples from this initial screening were further subjected to the analysis of specific transgenic material to determine the type of GMO present with subsequent quantification. Roundup Ready soya was found in eight samples, but its content was below the limit of 0.9%. None of the analysed sa

APA, Harvard, Vancouver, ISO, and other styles

38

Wang, Hongbin, Tao Wei, Xia Wang, et al. "Transcriptome Analyses from Mutant Salvia miltiorrhiza Reveals Important Roles for SmGASA4 during Plant Development." International Journal of Molecular Sciences 19, no. 7 (2018): 2088. http://dx.doi.org/10.3390/ijms19072088.

Full text

Abstract:

Salvia miltiorrhiza (S. miltiorrhiza) is an important Chinese herb that is derived from the perennial plant of Lamiaceae, which has been used to treat neurasthenic insomnia and cardiovascular disease. We produced a mutant S. miltiorrhiza (MT), from breeding experiments, that possessed a large taproot, reduced lateral roots, and defective flowering. We performed transcriptome profiling of wild type (WT) and MT S. miltiorrhiza using second-generation Illumina sequencing to identify differentially expressed genes (DEGs) that could account for these phenotypical differences. Of the DEGs identified

APA, Harvard, Vancouver, ISO, and other styles

39

Koh, Eun-Ji, Sung June Lee, Suk-Whan Hong, Hoi Seon Lee, and Hojoung Lee. "The ABA Effect on the Accumulation of an Invertase Inhibitor Transcript that Is Driven by the CAMV35S Promoter in ARABIDOPSIS." Molecules and Cells 26, no. 3 (2008): 236–42. http://dx.doi.org/10.1016/s1016-8478(23)13990-2.

Full text

APA, Harvard, Vancouver, ISO, and other styles

40

Zhao, Qingchun, Zhenzhen Luo, Jiadong Chen, et al. "Characterization of two cis-acting elements, P1BS and W-box, in the regulation of OsPT6 responsive to phosphors deficiency." Plant Growth Regulation 93, no. 3 (2021): 303–10. http://dx.doi.org/10.1007/s10725-020-00688-z.

Full text

Abstract:

AbstractPhosphorus (P) deficiency is one of the major nutrient stresses restricting plant growth. The uptake of P by plants from soil is mainly mediated by the phosphate (Pi) transporters belonging to the PHT1 family. Multiple PHT1 genes from diverse plant species have been shown to be strongly up-regulated upon Pi starvation, however, the underlying mechanisms for the Pi-starvation-induced (PSI) up-regulation have not been well deciphered for most Pi transporter genes. Here, we reported a detailed dissection of the promoter activity of a PSI rice Pi transporter gene OsPT6, using the β-glucuro

APA, Harvard, Vancouver, ISO, and other styles

41

Malnoy, M., Q. Jin, E. E. Borejsza-Wysocka, S. Y. He, and H. S. Aldwinckle. "Overexpression of the Apple MpNPR1 Gene Confers Increased Disease Resistance in Malus × domestica." Molecular Plant-Microbe Interactions® 20, no. 12 (2007): 1568–80. http://dx.doi.org/10.1094/mpmi-20-12-1568.

Full text

Abstract:

The NPR1 gene plays a pivotal role in systemic acquired resistance in plants. Its overexpression in Arabidopsis and rice results in increased disease resistance and elevated expression of pathogenesis-related (PR) genes. An NPR1 homolog, MpNPR1-1, was cloned from apple (Malus × domestica) and overexpressed in two important apple cultivars, Galaxy and M26. Apple leaf pieces were transformed with the MpNPR1 cDNA under the control of the inducible Pin2 or constitutive Cauliflower mosaic virus (CaMV)35S promoter using Agrobacterium tumefaciens. Overexpression of MpNPR1 mRNA was shown by reverse tr

APA, Harvard, Vancouver, ISO, and other styles

42

Zhang, J. I. Yi, Mary Sledge, Joseph Bouton, and Zeng-Y. U. Wang. "Increased cuticular wax accumulation and enhanced drought tolerance in transgenic alfalfa (Medicago sativa) by overexpression of a transcription factor gene." NZGA: Research and Practice Series 12 (January 1, 2006): 145–50. http://dx.doi.org/10.33584/rps.12.2006.3024.

Full text

Abstract:

Plant cuticular waxes play an important role in protecting aerial organs from damage caused by multiple environmental stresses such as drought, cold, UV radiation, pathogen infection, and insect attack. We characterized a novel AP2 domain-containing transcription factor gene, designated WXP1, from the model legume plant Medicago truncatula. The gene is able to activate wax production and confer drought tolerance in alfalfa (Medicago sativa). The predicted protein of WXP1 has 371 aa; it is one of the longest peptides of all the single AP2 domain proteins in M. truncatula. Transcript level of WX

APA, Harvard, Vancouver, ISO, and other styles

43

Petrillo, Caroline Pereira, Newton Portilho Carneiro, Antônio Álvaro Corsetti Purcino, Carlos Henrique Siqueira Carvalho, José Donizeti Alves, and Andréa Almeida Carneiro. "Optimization of particle bombardment parameters for the genetic transformation of Brazilian maize inbred lines." Pesquisa Agropecuária Brasileira 43, no. 3 (2008): 371–78. http://dx.doi.org/10.1590/s0100-204x2008000300012.

Full text

Abstract:

The objective of this work was to develop a genetic transformation system for tropical maize genotypes via particle bombardment of immature zygotic embryos. Particle bombardment was carried out using a genetic construct with bar and uidA genes under control of CaMV35S promoter. The best conditions to transform maize tropical inbred lines L3 and L1345 were obtained when immature embryos were cultivated, prior to the bombardment, in higher osmolarity during 4 hours and bombarded at an acceleration helium gas pressure of 1,100 psi, two shots per plate, and a microcarrier flying distance of 6.6 cm

APA, Harvard, Vancouver, ISO, and other styles

44

Topping, J. F., W. Wei, and K. Lindsey. "Functional tagging of regulatory elements in the plant genome." Development 112, no. 4 (1991): 1009–19. http://dx.doi.org/10.1242/dev.112.4.1009.

Full text

Abstract:

In comparison with animals, relatively few plant genes have been identified that have been shown to be under organ-, tissue- or cell-type-specific regulation. In this paper, we describe how the beta-glucuronidase (GUS) reporter gene (gusA or uidA), fused to a weak promoter (a truncated (−90 bp) CaMV35S promoter), can be used to identify tissue-specific markers in transgenic tobacco plants. The rationale was that the expression of gusA would be determined primarily by position effect. Quantitative analysis revealed that, of 184 −90-gus transgenic plants, 73% exhibited gusA gene activation in le

APA, Harvard, Vancouver, ISO, and other styles

45

Hằng, Phạm Thu, Đàm Quang Hiếu, Phan Tuấn Nghĩa, and Phạm Xuân Hội. "Construction of vector and transformation of drought-responsive gene OsNAC1 into J02 (Oryza sativa L. Japonica) rice." Vietnam Journal of Biotechnology 14, no. 2 (2016): 271–77. http://dx.doi.org/10.15625/1811-4989/14/2/9340.

Full text

Abstract:

NAC (including NAM - no apical meristem, ATAF1/2 - Arabidopsis transcription activation factor and CUC2 - cup-shaped cotyledon), which is the largest plant transcription factor family, plays an important role in development and stress responses in plants. Protein of this family is characterized by a highly conserved DNA binding domain, known as NAC domain in the N-terminal region. In contrast, the C-terminal region of NAC proteins, usually containing the transcriptional activation domain, is highly diversified both in length and sequence. More than 100 members of this family have been identifi

APA, Harvard, Vancouver, ISO, and other styles

46

Li, W., L. G. Li, X. F. Sun, and K. X. Tang. "An oleosin-fusion protein driven by the CaMV35S promoter is accumulated in Arabidopsis (Brassicaceae) seeds and correctly targeted to oil bodies." Genetics and Molecular Research 11, no. 3 (2012): 2138–46. http://dx.doi.org/10.4238/2012.august.13.1.

Full text

APA, Harvard, Vancouver, ISO, and other styles

47

Wu, Hui, Jin-song He, Fang Zhang, Jianfeng Ping, and Jian Wu. "Contamination-free visual detection of CaMV35S promoter amplicon using CRISPR/Cas12a coupled with a designed reaction vessel: Rapid, specific and sensitive." Analytica Chimica Acta 1096 (February 2020): 130–37. http://dx.doi.org/10.1016/j.aca.2019.10.042.

Full text

APA, Harvard, Vancouver, ISO, and other styles

48

Yi, Jung-Yoon, Hyo-Won Seo, Ji-Hong Cho, and Kuen-Woo Park. "067 Analysis of Protein Expression and Resistance to Bacteria in Transgenic Potatoes with Antimicrobial Gene." HortScience 35, no. 3 (2000): 400B—400. http://dx.doi.org/10.21273/hortsci.35.3.400b.

Full text

Abstract:

Anti-bacterial peptide gene (shiva) was introduced into potato plants to improve the resistance to bacterial diseases. The 21 potato clones were selected in the medium containg 50 mg/L kanamycin and 13 transformants were confirmed by GUS activity assay using 4-methylumbellyferyl glucuronide (MUG) and PCR by NPTII specific primer sets. A 0.5-kb band was confirmed by PCR in the most transformants of T0, T1, and T2 generations. As a result of PCR with primer set chosen at shiva and GUS, expected 690-bp fragments were produced in the most transformants of T0, T1, and T2 generations. For southern b

APA, Harvard, Vancouver, ISO, and other styles

49

BIHANI, P., B. CHAR, and S. BHARGAVA. "Transgenic expression of sorghum DREB2 in rice improves tolerance and yield under water limitation." Journal of Agricultural Science 149, no. 1 (2010): 95–101. http://dx.doi.org/10.1017/s0021859610000742.

Full text

Abstract:

SUMMARYPlant-specific transcription factors belonging to the dehydration response element binding (DREB)/C-repeat binding factor (CBF) subfamily of the AP2/EREBP family specifically interact with dehydration-responsive elements (DRE)/C-repeat (CRT) and control the expression of many stress-inducible genes in plants. Two major subgroups of DREB proteins are represented by DREB1 and DREB2, which are induced specifically under cold and drought/salt stress, respectively. A DREB2 transcription factor gene from sorghum, SbDREB2 was identified and cloned in binary vectors, such that it was driven eit

APA, Harvard, Vancouver, ISO, and other styles

50

Ravelonandro, Michel, Pascal Briard, Ralph Scorza, et al. "Robust Response to Plum pox virus Infection via Plant Biotechnology." Genes 12, no. 6 (2021): 816. http://dx.doi.org/10.3390/genes12060816.

Full text

Abstract:

Our goal was to target silencing of the Plum pox virus coat protein (PPV CP) gene independently expressed in plants. Clone C-2 is a transgenic plum expressing CP. We introduced and verified, in planta, the effects of the inverse repeat of CP sequence split by a hairpin (IRSH) that was characterized in the HoneySweet plum. The IRSH construct was driven by two CaMV35S promoter sequences flanking the CP sequence and had been introduced into C1738 plum. To determine if this structure was enough to induce silencing, cross-hybridization was made with the C1738 clone and the CP expressing but PPV-sus

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!