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Academic literature on the topic 'Cancer cell cultures'

Author: Grafiati

Published: 19 February 2023

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Journal articles on the topic "Cancer cell cultures"

Mahadevan, Swarna, Kenelm Kwong, Mingjie Lu, Elizabeth Kelly, Belal Chami, Yevgeniy Romin, Sho Fujisawa, Katia Manova, Malcolm A. S. Moore, and Hans Zoellner. "A Novel Cartesian Plot Analysis for Fixed Monolayers That Relates Cell Phenotype to Transfer of Contents between Fibroblasts and Cancer Cells by Cell-Projection Pumping." International Journal of Molecular Sciences 23, no. 14 (July 19, 2022): 7949. http://dx.doi.org/10.3390/ijms23147949.

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We recently described cell-projection pumping as a mechanism transferring cytoplasm between cells. The uptake of fibroblast cytoplasm by co-cultured SAOS-2 osteosarcoma cells changes SAOS-2 morphology and increases cell migration and proliferation, as seen by single-cell tracking and in FACS separated SAOS-2 from co-cultures. Morphological changes in SAOS-2 seen by single cell tracking are consistent with previous observations in fixed monolayers of SAOS-2 co-cultures. Notably, earlier studies with fixed co-cultures were limited by the absence of a quantitative method for identifying sub-populations of co-cultured cells, or for quantitating transfer relative to control populations of SAOS-2 or fibroblasts cultured alone. We now overcome that limitation by a novel Cartesian plot analysis that identifies individual co-cultured cells as belonging to one of five distinct cell populations, and also gives numerical measure of similarity to control cell populations. We verified the utility of the method by first confirming the previously established relationship between SAOS-2 morphology and uptake of fibroblast contents, and also demonstrated similar effects in other cancer cell lines including from melanomas, and cancers of the ovary and colon. The method was extended to examine global DNA methylation, and while there was no clear effect on SAOS-2 DNA methylation, co-cultured fibroblasts had greatly reduced DNA methylation, similar to cancer associated fibroblasts.

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MAS, Bezerra, Ferreira LAM, Kawasaki-Oyama RS, Nascimento MCA, Cuzziol CI, Castanhole-Nunes MMU, Pavarino EC, Maniglia JM, and Goloni-Bertollo EM. "Effectiveness of Hypoxia-Induced Accumulation of Cancer Stem Cells in Head and Neck Squamous Cell Carcinoma." Cancer Medicine Journal 3, S1 (November 30, 2020): 13–23. http://dx.doi.org/10.46619/cmj.2020.3.s1-1003.

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INTRODUCTION: The small number of cancer stem cells, which correspond to only 0.01% - 0.1% of total tumor cells, has been the biggest obstacle in understanding their biology and role in the origin and maintenance of tumors, their metastatic and recurrence potentials, and resistance to radio-chemotherapy. Therefore, promoting its accumulation will enable further studies and future advances in the diagnosis and treatment of head and neck cancer squamous cell carcinoma. OBJECTIVE: To induce cancer stem cell accumulation in primary cell cultures of head and neck squamous cell carcinoma using a hypoxia chamber. METHODS: Head and neck squamous cell carcinoma samples were cultured and subjected to hypoxia. Oxygen deprivation aimed to induce cancer stem cell accumulation. RESULTS: Immediately after hypoxia, the percentage of O2-deprived cancer stem cells increased 2-fold as compared to control. Surprisingly, new phenotyping performed 45 days after hypoxia showed a 9-fold increase in cancer stem cell percentage in cells that suffered hypoxia. Hypoxic cells showed an increase in spheroid formation when compared to control cells, as well as enhanced abilities in invasion and migration. CONCLUSION: Hypoxia was efficient in cancer stem cell accumulation. As cancer stem cells are a small number of cells within the tumor, promoting their accumulation will enable further studies and future advances in the diagnosis and treatment of head and neck cancer.

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Timofeeva, Sofia V., Oleg I. Kit, Svetlana Yu Filippova, Anastasia O. Sitkovskaya, Irina V. Mezhevova, Nadezhda V. Gnennaya, Tatiana V. Shamova, et al. "Effect of berberine on cancer cell motility in glioma, lung cancer, and prostate cancer cell cultures." Journal of Clinical Oncology 40, no. 16_suppl (June 1, 2022): e15079-e15079. http://dx.doi.org/10.1200/jco.2022.40.16_suppl.e15079.

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e15079 Background: Initially, berberine was used as an antimicrobial agent in traditional medicine, but its anticancer properties were later discovered. In addition to its cytotoxic effect, berberine also has the ability to inhibit cell motility, which has been demonstrated in some permanent cancer cell lines. The objective of this study was to assess berberine anti-migratory activity in permanent cell cultures compared to primary cell cultures which are generally thought to better reflect tumor characteristics. Methods: H1299 lung cancer, PC3 prostate cancer, and T98G glioma cells, as well as primary cell cultures of the corresponding cancer obtained in our laboratory, were planted in an amount of 15*104 cells per well of a 24-well plate (Biofil, China) in DMEM medium (Gibco, USA) supplemented with 10% FBS (HyClone, USA). After cell adhesion berberine was added in concentration 5 µM, and a wound healing assay was performed according to the standard procedure. Cell plates were continuously incubated and photographed in Lionheart FX imager (BioTek, USA) at 37°C and 5.0% CO2. The extent of cell migration was measured as the percentage reduction in wound area after 48 hours of incubation relative to baseline. Data are presented as Mean ± 95% confidence interval (n = 12). Results: The use of berberine at a concentration of 5 µM led to a significant decrease in cell motility in permanent cultures of lung cancer, glioma and prostate cancer. Namely, the reduction in the wound area after 48 hours of incubation with bereberine was 74.52±12.3% (compared with 94.56±6.2% in the control) in H1299 cell culture, 38.22±10.6% (compared with 83.89±15.5% in the control) in T98G cell culture, and 48.6±7.5% (compared with 69.56±8.1% in the control) in PC3 cell culture. The resulting difference between the control and experimental groups in permanent cell cultures was statistically significant at a significance level of 5% (df = 22). At the same time, the values of wound area reduction for primary cultures of the same cancers did not differ significantly in the control and under the influence of 5 μM berberine at the accepted level of significance. Namely, the reduction in the wound area after 48 hours of incubation with bereberine was 84.79±11.2% (compared with 81.47±15.3% in the control) in lung cancer primary cell culture, 94.64±5.1% (compared with 91.73±6.8% in the control) in glioma primary culture, and 62.63±5.8% (compared with 61.1±8.9% in the control) in prostate cancer primary cell culture. Conclusions: Permanent cell lines are more sensitive to berberine anti-migratory activity than primary cancer cell lines of the same localization.

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Nishi, Masaaki, Mitsuo Shimada, Kozo Yoshikawa, Jun Higashijima, Toshihiro Nakao, Chie Takasu, Shohei Eto, and Hiroki Teraoku. "Effect of light irradiation by light emitting diode on colon cancer cells and cancer stem cells." Journal of Clinical Oncology 33, no. 3_suppl (January 20, 2015): 271. http://dx.doi.org/10.1200/jco.2015.33.3_suppl.271.

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271 Background: Recent studies demonstrate the efficacy of irradiation from light emitting diodes (LED) for wound healing and anti-inflammation and anti-cancertherapies. However, little is known about the effects of LED in colon cancer cells. Furthermore, most solid cancers are believed to be initiated from and maintained by cancer stem cells (CSCs). The purpose of this study was to evaluate the biological response of human colon cancer cells and CSCs to LED irradiation. Methods: <Experiment 1, Effect of LED on normal cancer cells> Human colon cancer cells (HT29 or HCT116) were seeded onto laboratory dishes that were put on LED irradiation equipment with a 465 nm-, 525 nm-, or 635 nm-LED. Irradiation at 15 or 30 mW was performed 10 min/day, each day for 5 days. Cell counting kit8 was then used to measure cell viability. Apoptosis and expression of several mRNAs (caspase, MAPK and autophagy pathway) in HT29 cultures irradiated with 465 nm LED were evaluated via AnnexinV/PI and RT-PCR, respectively. <Experiment 2, Effect of LED on CSCs> HCT-116 cell line was cultured with fetal bovine serum in RPMI-1640 medium and its sphere was grown in serum-free non-adherent culture. Cancer sphere were seeded onto laboratory dishes that were then put on LED irradiation equipment with a 465 nm-LED. Stemness gene and cell surface markers of CSCs expressions (Oct4, CD44, Nanog, EPCAM) were analyzed by RT-PCR. Results: <Experiment 1> Viability of HT29 and HCT116 cells was lower in 465 nm-LED irradiated cultures than in control cultures, but viability of HT29 cells did not differ between control cultures and 525 nm-LED or 635 nm-LED irradiated cultures. Moreover, the expression of FAS, caspase3, capase8, and JUK were significantly higher in 465 nm-LED irradiated cultures than in control cultures, and expression of ERK1/2 and LC3 was lower in blue-irradiated cells. <Experiment 2> Cell viability of cancer sphere were significantly decreased by LED irradiation(p<0.05). And, stemness gene and cell surface markers of CSCs expressions (Oct4, CD44, Nanog, EPCAM) were significantly decreased by LED irradiation (p<0.05) Conclusions: LED irradiation at 465 nm inhibited the proliferation of both normal colon cancer cell lines and CSCs.

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Gout, Peter W., Robert L. Noble, and Charles T. Beer. "Cultured Nb rat lymphoma cells in endocrine and cancer research." Biochemistry and Cell Biology 64, no. 7 (July 1, 1986): 659–66. http://dx.doi.org/10.1139/o86-091.

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This review outlines the establishment, properties, and use of two lines of cultured Nb rat lymphoma cells. The cultured cells have retained important properties of the cancers of origin, such as dependency on prolactin for growth and a high sensitivity to antineoplastic Vinca alkaloids. The cultures have been useful for defining the hormonal dependency of the lymphomas in the animal and for studying the progression of the lymphomas from hormonal dependency to autonomy. A new, specific and highly sensitive in vitro bioassay for lactogenic hormones has been developed from one of the cultures. The use of the lymphoma cell cultures has revealed unsuspected pharmacological differences between the closely related, clinically useful antineoplastic Vinca alkaloids, vinblastine and vincristine. The lymphoma cell cultures are also useful tools for studying biochemical, cell cycle related events which follow the mitogenic stimulation of cells by a polypeptide growth factor.

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Koch, Jana, Dina Mönch, Annika Maaß, Christian Gromoll, Thomas Hehr, Tobias Leibold, Hans J. Schlitt, Marc-H. Dahlke, and Philipp Renner. "Three dimensional cultivation increases chemo- and radioresistance of colorectal cancer cell lines." PLOS ONE 16, no. 1 (January 4, 2021): e0244513. http://dx.doi.org/10.1371/journal.pone.0244513.

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Although 2D cell cultures are commonly used to predict therapy response, it has become clear that 3D cultures may better mimic the in vivo situation and offer the possibility of tailoring translational clinical approaches. Here, we compared the response of 2D and 3D colorectal cancer (CRC) cell lines to irradiation and chemotherapy. Classic 2D cultures and 3D spheroids of CRC cell lines (CaCo2, Colo205, HCT116, SW480) were thoroughly established, then irradiated with doses of 1, 4, or 10 Gy, using a clinical-grade linear accelerator. The response was assessed by immunohistochemistry, flow cytometry, and TUNEL assays. Upon irradiation, CRC 3D spheroids were morphologically altered. After irradiation with 10 Gy, annexin V/PI staining revealed a 1.8- to 4-fold increase in the apoptosis rate in the 2D cell cultures (95% CI 3.24±0.96), and a 1.5- to 2.4-fold increase in the 3D spheroids (95% CI 1.56±0.41). Irradiation with 1 Gy caused 3- and 4-fold increases in TUNEL positive cells in the CaCo2 and HCT116 (p = 0.01) 2D cultures, respectively, compared with a 2-fold increase in the 3D spheroids. Furthermore, the 2D and 3D cultures responded differently to chemotherapy; the 3D cultures were more resistant to 5-FU and cisplatin, but not to doxorubicin and mitomycin C, than the 2D cultures. Taken together, CRC cells cultured as 3D spheroids displayed markedly higher resistance to irradiation therapy and selected chemotherapeutic drugs than 2D cultures. This in vitro difference must be considered in future approaches for determining the ideal in vitro systems that mimic human disease.

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Sieni, Elisabetta, Monica Dettin, Mariangela De Robertis, Bianca Bazzolo, Maria Teresa Conconi, Annj Zamuner, Ramona Marino, Flavio Keller, Luca Giovanni Campana, and Emanuela Signori. "The Efficiency of Gene Electrotransfer in Breast-Cancer Cell Lines Cultured on a Novel Collagen-Free 3D Scaffold." Cancers 12, no. 4 (April 23, 2020): 1043. http://dx.doi.org/10.3390/cancers12041043.

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Gene Electro-Transfer (GET) is a powerful method of DNA delivery with great potential for medical applications. Although GET has been extensively studied in vitro and in vivo, the optimal parameters remain controversial. 2D cell cultures have been widely used to investigate GET protocols, but have intrinsic limitations, whereas 3D cultures may represent a more reliable model thanks to the capacity of reproducing the tumor architecture. Here we applied two GET protocols, using a plate or linear electrode, on 3D-cultured HCC1954 and MDA-MB231 breast cancer cell lines grown on a novel collagen-free 3D scaffold and compared results with conventional 2D cultures. To evaluate the electrotransfer efficiency, we used the plasmid pEGFP-C3 encoding the enhanced green fluorescent protein (EGFP) reporter gene. The novel 3D scaffold promoted extracellular matrix deposition, which particularly influences cell behavior in both in vitro cell cultures and in vivo tumor tissue. While the transfection efficiency was similar in the 2D-cultures, we observed significant differences in the 3D-model. The transfection efficiency in the 3D vs 2D model was 44% versus 15% (p < 0.01) and 24% versus 17% (p < 0.01) in HCC1954 and MDA-MB231 cell cultures, respectively. These findings suggest that the novel 3D scaffold allows reproducing, at least partially, the peculiar morphology of the original tumor tissues, thus allowing us to detect meaningful differences between the two cell lines. Following GET with plate electrodes, cell viability was higher in 3D-cultured HCC1954 (66%) and MDA-MB231 (96%) cell lines compared to their 2D counterpart (53% and 63%, respectively, p < 0.001). Based on these results, we propose the novel 3D scaffold as a reliable support for the preparation of cell cultures in GET studies. It may increase the reliability of in vitro assays and allow the optimization of GET parameters of in vivo protocols.

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Betriu, Nausika, Anna Andreeva, and Carlos E. Semino. "Erlotinib Promotes Ligand-Induced EGFR Degradation in 3D but Not 2D Cultures of Pancreatic Ductal Adenocarcinoma Cells." Cancers 13, no. 18 (September 7, 2021): 4504. http://dx.doi.org/10.3390/cancers13184504.

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The epithelial growth factor receptor (EGFR) is a tyrosine kinase receptor that participates in many biological processes such as cell proliferation. In addition, EGFR is overexpressed in many epithelial cancers and therefore is a target for cancer therapy. Moreover, EGFR responds to lots of stimuli by internalizing into endosomes from where it can be recycled to the membrane or further sorted into lysosomes where it undergoes degradation. Two-dimensional cell cultures have been classically used to study EGFR trafficking mechanisms in cancer cells. However, it has been widely demonstrated that in 2D cultures cells are exposed to a non-physiological environment as compared to 3D cultures that provide the normal cellular conformation, matrix dimensionality and stiffness, as well as molecular gradients. Therefore, the microenvironment of solid tumors is better recreated in 3D culture models, and this is why they are becoming a more physiological alternative to study cancer physiology. Here, we develop a new model of EGFR internalization and degradation upon erlotinib treatment in pancreatic ductal adenocarcinoma (PDAC) cells cultured in a 3D self-assembling peptide scaffold. In this work, we show that treatment with the tyrosine kinase inhibitor erlotinib promotes EGFR degradation in 3D cultures of PDAC cell lines but not in 2D cultures. We also show that this receptor degradation does not occur in normal fibroblast cells, regardless of culture dimensionality. In conclusion, we demonstrate not only that erlotinib has a distinct effect on tumor and normal cells but also that pancreatic ductal adenocarcinoma cells respond differently to drug treatment when cultured in a 3D microenvironment. This study highlights the importance of culture systems that can more accurately mimic the in vivo tumor physiology.

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Gozdz, Agata, Bartosz Wojtaś, Patrycja Szpak, Paulina Szadkowska, Tomasz Czernicki, Andrzej Marchel, Katarzyna Wójtowicz, et al. "Preservation of the Hypoxic Transcriptome in Glioblastoma Patient-Derived Cell Lines Maintained at Lowered Oxygen Tension." Cancers 14, no. 19 (October 4, 2022): 4852. http://dx.doi.org/10.3390/cancers14194852.

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Despite numerous efforts aiming to characterise glioblastoma pathology (GBM) and discover new therapeutic strategies, GBM remains one of the most challenging tumours to treat. Here we propose the optimisation of in vitro culturing of GBM patient-derived cells, namely the establishment of GBM-derived cultures and their maintenance at oxygen tension mimicking oxygenation conditions occurring within the tumour. To globally analyse cell states, we performed the transcriptome analysis of GBM patient-derived cells kept as spheroids in serum-free conditions at the reduced oxygen tension (5% O2), cells cultured at atmospheric oxygen (20% O2), and parental tumour. Immune cells present in the tumour were depleted, resulting in the decreased expression of the immune system and inflammation-related genes. The expression of genes promoting cell proliferation and DNA repair was higher in GBM cell cultures when compared to the relevant tumour sample. However, lowering oxygen tension to 5% did not affect the proliferation rate and expression of cell cycle and DNA repair genes in GBM cell cultures. Culturing GBM cells at 5% oxygen was sufficient to increase the expression of specific stemness markers, particularly the PROM1 gene, without affecting neural cell differentiation markers. GBM spheroids cultured at 5% oxygen expressed higher levels of hypoxia-inducible genes, including those encoding glycolytic enzymes and pro-angiogenic factors. The genes up-regulated in cells cultured at 5% oxygen had higher expression in parental GBMs compared to that observed in 20% cell cultures, suggesting the preservation of the hypoxic component of GBM transcriptome at 5% oxygen and its loss in standard culture conditions. Evaluation of expression of those genes in The Cancer Genome Atlas dataset comprising samples of normal brain tissue, lower-grade gliomas and GBMs indicated the expression pattern of the indicated genes was specific for GBM. Moreover, GBM cells cultured at 5% oxygen were more resistant to temozolomide, the chemotherapeutic used in GBM therapy. The presented comparison of GBM cultures maintained at high and low oxygen tension together with analysis of tumour transcriptome indicates that lowering oxygen tension during cell culture may more allegedly reproduce tumour cell behaviour within GBM than standard culture conditions (e.g., atmospheric oxygen tension). Low oxygen culture conditions should be considered as a more appropriate model for further studies on glioblastoma pathology and therapy.

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Fontana, Fabrizio, Michela Raimondi, Monica Marzagalli, Michele Sommariva, Nicoletta Gagliano, and Patrizia Limonta. "Three-Dimensional Cell Cultures as an In Vitro Tool for Prostate Cancer Modeling and Drug Discovery." International Journal of Molecular Sciences 21, no. 18 (September 16, 2020): 6806. http://dx.doi.org/10.3390/ijms21186806.

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In the last decade, three-dimensional (3D) cell culture technology has gained a lot of interest due to its ability to better recapitulate the in vivo organization and microenvironment of in vitro cultured cancer cells. In particular, 3D tumor models have demonstrated several different characteristics compared with traditional two-dimensional (2D) cultures and have provided an interesting link between the latter and animal experiments. Indeed, 3D cell cultures represent a useful platform for the identification of the biological features of cancer cells as well as for the screening of novel antitumor agents. The present review is aimed at summarizing the most common 3D cell culture methods and applications, with a focus on prostate cancer modeling and drug discovery.

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Dissertations / Theses on the topic "Cancer cell cultures"

Park, Deric M., Jinkyu Jung, Jimmy Masjkur, Stylianos Makrogkikas, Doreen Ebermann, Sarama Saha, Roberta Rogliano, et al. "Hes3 regulates cell number in cultures from glioblastoma multiforme with stem cell characteristics." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2013. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-127014.

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Tumors exhibit complex organization and contain a variety of cell populations. The realization that the regenerative properties of a tumor may be largely confined to a cell subpopulation (cancer stem cell) is driving a new era of anti-cancer research. Cancer stem cells from Glioblastoma Multiforme tumors express markers that are also expressed in non-cancerous neural stem cells, including nestin and Sox2. We previously showed that the transcription factor Hes3 is a marker of neural stem cells, and that its expression is inhibited by JAK activity. Here we show that Hes3 is also expressed in cultures from glioblastoma multiforme which express neural stem cell markers, can differentiate into neurons and glia, and can recapitulate the tumor of origin when transplanted into immunocompromised mice. Similar to observations in neural stem cells, JAK inhibits Hes3 expression. Hes3 RNA interference reduces the number of cultured glioblastoma cells suggesting a novel therapeutic strategy.

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Walls, G. A. "A study of cellular heterogeneity and therapeutic resistance in cultures of human lung cancer." Thesis, University of Cambridge, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.380720.

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Kalluri, Usha. "Gas chromatographic-mass spectrometry analysis of volatile organic compounds from cancer cell cultures - The effect of hypoxia." Thesis, Federation University Australia, 2018. http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/166534.

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Early diagnosis of lung cancer improves patient outcomes which has led to a search for non-invasive diagnostic tests suitable for population screening. Volatile organic compounds (VOCs) in exhaled breath have shown potential, however, confirmation of the metabolic origins and disease specificity of candidate markers is required. Cell culture metabolomics can identify disease biomarkers and their origins. To date VOC profiles from in vitro cultured cancer cells have little similarity to cancer breath VOC profiles. In vivo, cancer cells experience hypoxia whereas in vitro cells are cultured under normoxic conditions. Since hypoxia influences cell metabolism, we hypothesize that cancer cells cultured under hypoxic conditions will have altered cell metabolism and produce VOC profiles more typical of cancer breathe. This study investigates the effect of hypoxia on metabolic reprogramming in A549 lung cancer cells cultured under standard normoxic (atmospheric oxygen) or hypoxic (2% oxygen) conditions. Results from quantitative RT-PCR demonstrated a significant upregulation in hypoxia of the glucose transporter (GLUT1) and the key TCA regulatory gene PDHK1, demonstrating that hypoxia plays a pivotal role in regulating metabolism in A549 cells. A ratio-metric assessment of Lipid Peroxidation (LPO) and the production of reactive oxygen species (ROS) showed an increase in LPO and a slight decrease in the production of ROS in hypoxic cultures, the combined effect of which may serve to equip the cells to adapt to and proliferate under low oxygen. Finally, the comparison of endogenous VOCs produced by A549 cells under hypoxic and normoxic conditions identified twelve VOCs unique to cells grown under hypoxic conditions including n-pentane, a marker of LPO and cancer, and 3-methyl hexane, which has been reported as a biomarker of cancer. This data is consistent with the hypothesis that a hypoxic tumour microenvironment may influence cell metabolism leading to a unique and diagnostic cancer VOC profile.
Doctor of Philosophy

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Roberts, Simon A. "Studies on the toxicity and metabolism of T-2 toxin in keratinocyte cultures : evaluation of a keratinocyte cell line and primary cultures as model systems for toxicity testing." Thesis, University of Surrey, 1989. http://epubs.surrey.ac.uk/847950/.

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With a view to establishing a model system for examining toxicity in skin, primary lingual keratinocyte cultures , a keratinocyte cell line and freshly isolated keratinocytes all derived from rat sub-lingual epithelium, were partially characterized both morphologically and enzymically and the toxicity and metabolism of the mycotoxin T-2, studied therein. A number of techniques for obtaining pure suspensions of sublingual keratinocytes (NCK) were examined and dispase is recommended for completely separating the epithelium from its dermis prior to trypsinization. Existing methods for the culture of PLK cells were improved to reduce the number of rats used and minimize the fibroblast contamination and a technique for culturing the epithelium associated with human hair follicles was also examined. The follicle technique was found to produce primary cultures which were 100% epithelial but considerable time and resources were required to generate relatively few cells. The keratinocyte cultures, PLK and RTE5, were shown to produce keratin and undergo stratification like the epithelium in vivo, Using a series of specific enzyme inhibitors, both the cultured and non-cultured epithelial cells were found to possess the same characteristic forms of acetylcholinesterase, butyrylcholinesterase and carboxylesterase. The effect of T-2 on protein synthesis in the keratinocyte cells was examined and a dose-related inhibition was evident. The primary and freshly isolated cells appeared to be the most resistant to synthesis inhibition. The rate of recovery from this inhibition was greatest in the cell line which also lost T-2 at the fastest rate. The uptake into and loss of T-2 from the keratinocyte cells was chiefly by simple diffusion, but studies using rotenone demonstrated that an active process may have been involved in reducing the rates of uptake and loss. The total amount of T-2 absorbed was likely to be dependent on the number of binding sites while the overall rate of loss was dependent on the strength of that binding. The possible nature of these binding sites is discussed. T-2 was metabolized in all the keratinocyte preparations to the same products found in mammalian skin, in vivo, and studies with specific enzyme inhibitors showed that a carboxylesterase was most likely to be involved in its hydrolysis. The greater metabolism of T-2 in the primary cells, which contained the most carboxylesterase, is likely to have been one of the factors involved in reducing the levels of protein synthesis inhibition in these cells. Rat sublingual epithelium and the cultures derived from it have a similar morphology and esterolytic capability to that of skin in viva. The PLK cells were most similar to NCK cells in terms of protein synthesis and esterolytic capability, so if it is assumed that NCK cells are representative of the sublingual epithelial cells in viva and hence skin, then the primary cultures might prove to be the best model culture system for studying toxicity in skin. Nevertheless, the RTE5 cell line was most similar to the NCK cells in respect of T-2 metabolism, and if it shown to have other characteristic skin metabolism systems then it too might prove useful for studying skin toxicity. In addition, the cell line would probably prove to be a more convenient, simpler, reproducible and cheaper means of examining skin toxicity on a large scale.

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Laryea, D., A. Isaksson, Colin W. Wright, R. Larsson, and P. Nygren. "Characterization of the cytotoxic activity of the indoloquinoline alkaloid cryptolepine in human tumour cell lines and primary cultures of tumour cells from patients." Springer, 2009. http://hdl.handle.net/10454/4533.

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The plant derived indoloquinoline alkaloid cryptolepine was investigated for its cytotoxic properties in 12 human tumour cell lines and in primary cultures of tumour cells from patients. The fluorometric microculture cytotoxicity assay was used to assess cytotoxicity and DNA mocro-array analysis to evaluate gene expression. Cryptolepine mean IC50 in the cell line panel was 0.9 microM compared with 1.0 and 2.8 microM in haemaotological and solid tumour malignancies respectively. Among patient solid tumour samples, those from breast cancer were the most sensitive and essentially as sensitive as haematological malignancies, respectively. Among patient solid tumour samples, those from breast cancer were the most sensitive and essentially as senstive as haematological malignancies. Cryptolepine activity showed highest correlations to topoisomerase II and microtubule targetting drugs. In the cell lines cryptolepine activity was essentially unaffected by established mechanisms of drug resistance. A number of genes were identified as associated with cryptolepine activity. In conclusion, cryptolepine shows interesting in vitro cytotoxic properties and its further evaluation as an anticancer drug seems warranted.

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Bates, Amber Marie. "Matrix metalloproteinase and cytokine profiles from cell co-cultures and their role in oral inflammation and head and neck cancer." Diss., University of Iowa, 2018. https://ir.uiowa.edu/etd/6051.

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Cytokines, chemokines, and MMPs play a major role in both the inflammatory and tumor microenvironments. However, there has been very little research focused on characterizing the MMP and cytokine responses of co-culture models constructed with cells from the oral cavity to study disease. Cells grown in single-cell cultures do not receive signals from other cells as they do in the natural host environment, and the results from single-cell culture studies are often not representative of the host response. Accordingly, studies involving cell cultures with multiple cell types are needed to better represent the host response and responses seen in clinical situation. Therefore, we have developed multiple 3D transwell co-culture systems to study mechanisms involved in periodontal disease and head and neck cancer. Using a unique 3D transwell co-culture, we are able to eliminate cell-cell physical interactions and focus on the effects of cell signals and cell reactions to these signals caused by the presence other cells in the co-culture. Studying the MMP and immunosuppressive biomarker response in this type of model will provide new insights on the tumor microenvironment created by head and neck cancer cells and the pro-inflammatory environment in periodontal disease.

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Kashtl, Ghasaq J. "Differential membrane-type matrix metalloproteinase expression in phenotypically defined breast cancer cell lines: Comparison of MT-MMP expression in environmentally-challenged 2D monolayer cultures and 3D multicellular tumour spheroids." Thesis, University of Bradford, 2018. http://hdl.handle.net/10454/17346.

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Matrix metalloproteinases (MMPs) are a family of zinc endopeptidases capable of digesting the extracellular matrix (ECM), which is essential for tissue structure and transmitting messages between cells. MMPs play an important role in cancer, controlling cell migration, proliferation, apoptosis, regulation of tumour expansion, angiogenesis and invasion. Previous research has indicated high expression of MT1-MMP in breast cancers suggesting a potential role in tumour progression. Our results confirm that 3D multicellular tumour spheroids (MCTS) using phenotype-specific breast cancer cell lines are a valuable experimental model of the tumour microenvironment. Optimisation of MCTS culture growth conditions using different breast cancer cell lines (MCF-7, T47D, MDA-MB-468 and MDA-MB-231) was performed. Unexpected detection of MT1-MMP in MCF-7 MCTS warranted further investigation. MT1-MMP expression in different micro-environmental conditions, including hypoxia and nutrient deprivation (serum-free induced autophagy) were measured in MCF-7 monolayer cultures and MCTS models using immunofluorescence (IF), immunohistochemistry (IHC) and western blot (WB). MT1-MMP expression was rapidly and irreversibly up-regulated in MCF-7 breast cancer cells under conditions of stress (hypoxia and autophagy) compared to normal conditions suggesting an important role of the culture environment on cells behaviour and protein expression. We employed isobaric tags for relative and absolute quantitation (iTRAQ) technology to correlate MT1-MMP increase with proteomic profiles in MCF-7 breast cancer cell grown under hypoxic, serum-free and 3D MCTS conditions. More than 3500 proteins were identified, which were clustered into groups based on response to unique or shared microenvironment changes. Hypoxic monolayer and spheroid cells exhibited changes in anaerobic metabolism and lipid synthesis, respectively, whereas autophagy resulted in up-regulation of cellular component disassembly. The result indicated multiple drivers of MT1-MMP expression in MCF-7 cells.
Al-Mstansiriya University, Iraq

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Trakarnpaibul-Kunakornsawat, Sunee. "Effects of 1α,25-Dihydroxyvitamin D₃ and its analogs in Cancer-associated Hypercalcemia in vivo and in vitro, and in normal Prostate Epithelial and Stromal Primary Cell Cultures /." The Ohio State University, 2001. http://rave.ohiolink.edu/etdc/view?acc_num=osu1486572165276222.

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Dolega, Monika Elzbieta. "Developement of microtechnologies for 3D cell culture to study prostate acini formation and carcinogenesis." Thesis, Grenoble, 2014. http://www.theses.fr/2014GRENS022/document.

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Tout épithélium glandulaire sécrétoire est constitué d'une unité structurale et fonctionnelle commune, l'acinus. C'est une architecture sphérique pluricellulaire parfaitement différentiée et polarisée qui, reconstruite en culture 3D, mime l'organisation réelle du tissu. Etudier les déterminants environnementaux et génétiques qui gouvernent la transformation d'un acinus en sphéroïde s'apparentant à une tumeur est l'un des enjeux majeurs des modèles in vitro. Un des défis actuels est d'adapter ces modèles in vitro à des conditions de culture 3D qui soient compatibles avec la réalisation de cribles génétiques en 3D, basés par exemple sur l'ARN interférence (RNAi). Cependant, les formats standards de culture 3D et les méthodes analytiques ne sont pas compatibles aux cribles haut-débit. Ils ne permettent pas de contrôler la taille et la distribution des acini, sont dépendants d'immuno-marquages et les acquisitions sont longues. Par ailleurs, la microscopie confocale et vidéomicroscopie offrent un champ d'observation restreint qui ne permet pas d'observer un grand nombre de structures 3D en même temps, pour permettre une analyse statistique. Ainsi, dans le but i) de développer des modèles cellulaires appropriés en 3D, ii) d'adresser des questions fondamentales relatives au cancer de la prostate et iii) de réaliser des cribles RNAi dans un contexte plus pertinent que la culture 2D, j'ai développé des outils innovants au format microsystèmes adaptés à l'analyse haut-débit d'un grand nombre d'objets 3D. En optimisant les conditions de culture cellulaire 3D sur le modèle de la lignée cellulaire RWPE1, j'ai pu récapituler les étapes de formation des acini prostatiques et montrer que la formation du lumen est indépendante de la polarité et est gouvernée par deux mécanismes, « hollowing » et cavitation
In all secretory epithelia from glandular tissues, there is a common structural and functional unit, the acinus. It is a well polarized and organized pluricellular structure that is spontaneously reconstructed in 3D culture, therefore closely mimics the real structure we find in vivo. For my purpose, acini are used as models for tumor initiation and cancer development. One of the objectives of Biomics laboratory is to identify the genetic and microenvironmental determinants of prostate acini morphogenesis and polarity. The strategy is based on High-Throughput (HT) RNA interference (RNAi)-based screening. To meet this objective, my project was to develop appropriate 3D cell models which closely mimic the cyst-like and duct-like structure of prostate. By optimizing conventional 3D culture in Matrigel, I could recapitulate prostate acini morphogenesis and showed that lumen formation is independent to the polarity, which appears later. However, the conventional 3D cell culture formats and analytical tools are not suited for HT Screening (HTS). They lack control over acini size, are label-dependant and therefore time-consuming and labor intensive. Also, classical microscopy offers a very limited field of view and hence does not allow observing a large amount of 3D structures for statistical analysis

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Huynh, Minh Diem. "Reduction in apparent stromal cell culture density through transient fusions with osteosarcoma cells." University of Sydney, 2007. http://hdl.handle.net/2123/4465.

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Doctor of Philosophy
Benign tumours grow by expanding and displacing the surrounding tissues, while malignant tumours replace and destroy the surrounding tissues by invasion. Although there is extensive literature on mechanisms of tumour invasion and metastasis, with an emphasis on angiogenesis, adhesion, degradation of the extracellular matrix and migration, an important question not clearly addressed by the literature, but nonetheless approached in this thesis, is that of the fate of normal cells during tissue replacement by migrating invasive malignant cells. Earlier work in the laboratory where this PhD candidature was carried out, investigated the effect of osteosarcoma cells on endothelium. In contrast to the expected angiogenic effect of malignant cells for endothelium, it was found that the human osteosarcoma cell line (SAOS-2) induced apoptosis in human umbilical vein endothelial cells (HUVEC) in contact dependent manner (McEwen et al., 2003). It was suggested that apoptosis of endothelium by malignant tumour cells may facilitate tumour invasion and metastasis (McEwen et al., 2003), and one of the aims of the current study was to extend these findings to include human gingival fibroblasts (HGF) and human umbilical artery smooth muscle cells (HUASMC). The major finding of this thesis was that SAOS-2 induced a reduction in the apparent cell culture density of HGF and HUASMC in a contact-dependent manner. The SW480 colorectal carcinoma cell line did not have any clear effect upon the apparent stromal cell culture density of either HGF or HUASMC, suggesting that the effect under investigation was tumour cell line specific. Surprisingly and in contrast to the similar effect reported for endothelium (Chen et al., 2005; McEwen et al., 2003), the effect of SAOS-2 upon HGF and HUASMC was not due to stromal cell apoptosis. Apoptosis was ruled out as a possible mechanism for the reduced apparent culture density under study, by using widely accepted methods which are dependent upon intermucleosomal fragmentation of DNA, the permeability of plasma membranes to dyes in advanced apoptosis and necrosis, phosphatidylserine translocation as well as inhibitor studies blocking both caspase dependent and independent pathways. While apoptosis was not demonstrated, the possibility emerged that reduced apparent stromal cell culture density reflected fusion events rather than the simple removal of cells as had been earlier reported for HUVEC (McEwen et al., 2003). This idea was supported by reduced SAOS-2 circularity in co-culture. Confocal microscopy of cells pre-labelled with fluorescent dyes further supported this idea, with dual-labelling as evidence of cell fusion. Although occasional homotypic fusion of stromal cells was seen, heterotypic fusion of stromal cells with SAOS-2 was much more prevalent. Time lapse microscopy was performed to further characteristic cell fusion in co-cultures, and revealed multiple transient fusions between SAOS-2 and HGF. To work towards determining the biological relevance of the key observation, two stable SAOS-2 GFP clones were generated for future planned studies using human gingival explants and nude mice. Importantly, the clones were similar to native SAOS-2 with regard to alkaline phosphatise expression and reducing apparent stromal cell culture density. Transient fusions between HGF and SAOS-2, may be a mechanism for cooption of stromal cells into the malignant process, facilitating tumour invasion. Additionally, heterocellular fusion of SAOS-2 with stromal cells may facilitate immune evasion, while it seems likely that despite the absence of an identical activity in SW480 cells, other malignant tumour cells may also express similar activity.

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Books on the topic "Cancer cell cultures"

Langdon, Simon P. Cancer Cell Culture. New Jersey: Humana Press, 2003. http://dx.doi.org/10.1385/1592594069.

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Cree, Ian A., ed. Cancer Cell Culture. Totowa, NJ: Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-080-5.

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W, Masters John R., and Palsson Bernhard, eds. Cancer cell lines. Dordrecht: Kluwer Academic Publishers, 1999.

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Rolf, Bjerkvig, ed. Spheroid culture in cancer research. Boca Raton, Fla: CRC Press, 1992.

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Cancer cell culture: Methods and protocols. 2nd ed. Totowa, N.J: Humana Press, 2011.

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A conspiracy of cells: One woman's immortal legacy and the medical scandal it caused. Albany: State University of New York Press, 1986.

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Gogichadze, G. K. Karyogamic theory of cancer cell formation from the view of the XXI century. Hauppauge, N.Y: Nova Science Publishers, 2009.

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T, Gogichadze, ed. Karyogamic theory of cancer cell formation from the view of the XXI century. New York: Nova Biomedical Books, 2010.

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H, Kim Steve, ed. Molecular genetics in surgical oncology. Austin: R.G. Landes, 1994.

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Bessmertnaia zhizn' Genrietty Laks. Moskva: Kar'era Press, 2010.

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Book chapters on the topic "Cancer cell cultures"

Moissoglu, Konstadinos, Stephen J. Lockett, and Stavroula Mili. "Visualizing and Quantifying mRNA Localization at the Invasive Front of 3D Cancer Spheroids." In Cell Migration in Three Dimensions, 263–80. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2887-4_16.

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AbstractLocalization of mRNAs at the front of migrating cells is a widely used mechanism that functionally supports efficient cell movement. It is observed in single cells on two-dimensional surfaces, as well as in multicellular three-dimensional (3D) structures and in tissue in vivo. 3D multicellular cultures can reveal how the topology of the extracellular matrix and cell-cell contacts influence subcellular mRNA distributions. Here we describe a method for mRNA imaging in an inducible system of collective cancer cell invasion. MDA-MB-231 cancer cell spheroids are embedded in Matrigel, induced to invade, and processed to image mRNAs with single-molecule sensitivity. An analysis algorithm is used to quantify and compare mRNA distributions at the front of invasive leader cells. The approach can be easily adapted and applied to analyze RNA distributions in additional settings where cells polarize along a linear axis.

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Collins, Anne T. "Methodologies Applied to Establish Cell Cultures in Prostate Cancer." In Methods in Molecular Biology, 55–66. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7845-8_3.

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Mukherjee, Nabanita, Karoline A. Lambert, David A. Norris, and Yiqun G. Shellman. "Enrichment of Melanoma Stem-Like Cells via Sphere Assays." In Methods in Molecular Biology, 185–99. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1205-7_14.

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AbstractSphere assays are widely used in vitro techniques to enrich and evaluate the stem-like cell behavior of both normal and cancer cells. Utilizing three-dimensional in vitro sphere culture conditions provide a better representation of tumor growth in vivo than the more common monolayer cultures. We describe how to perform primary and secondary sphere assays, used for the enrichment and self-renewability studies of melanoma/melanocyte stem-like cells. Spheres are generated by growing melanoma cells at low density in nonadherent conditions with stem cell media. We provide protocols for preparing inexpensive and versatile polyHEMA-coated plates, setting up primary and secondary sphere assays in almost any tissue culture format and quantification methods using standard inverted microscopy. Our protocol is easily adaptable to laboratories with basic cell culture capabilities, without the need for expensive fluidic instruments.

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Watson, Geoffrey Alan, Kirsty Taylor, and Lillian L. Siu. "Innovation and Advances in Precision Medicine in Head and Neck Cancer." In Critical Issues in Head and Neck Oncology, 355–73. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-63234-2_24.

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AbstractThe clinical utility of precision medicine through molecular characterization of tumors has been demonstrated in some malignancies, especially in cases where oncogenic driver alterations are identified. Next generation sequencing data from thousands of patients with head and neck cancers have provided vast amounts of information about the genomic landscape of this disease. Thus far, only a limited number of genomic alterations have been druggable, such as NTRK gene rearrangements in salivary gland cancers (mainly mammary analogue secretory carcinoma), NOTCH mutations in adenoid cystic cancers, HRAS mutations in head and neck squamous cell cancers, and even a smaller number of these have reached regulatory approval status. In order to expand the scope of precision medicine in head and neck cancer, additional evaluation beyond genomics is necessary. For instance, there is increasing interest to perform transcriptomic profiling for target identification. Another advance is in the area of functional testing such as small interfering RNA and drug libraries on patient derived cell cultures. Liquid biopsies to detect specific tumor clones or subclones, or viral sequences such as HPV, are of great interest to enable non-invasive tracking of response or resistance to treatment. In addition, precision immuno-oncology is a tangible goal, with a growing body of knowledge on the interactions between the host immunity, the tumor and its microenvironment. Immuno-oncology combinations that are tailored to immunophenotypes of the host-tumor-microenvironment triad, personalized cancer vaccines, and adoptive cell therapies, among others, are in active development. Many therapeutic possibilities and opportunities lie ahead that ultimately will increase the reality of precision medicine in head and neck cancer.

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Catterall, Rachel, Reem Kurdieh, and Luke McCaffrey. "Studying Cell Polarity Dynamics During Cancer Initiation Using Inducible 3D Organotypic Cultures." In Methods in Molecular Biology, 455–66. New York, NY: Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2035-9_26.

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Christgen, Matthias, Matthias Ballmaier, Ulrich Lehmann, and Hans Kreipe. "Detection of Putative Cancer Stem Cells of the Side Population Phenotype in Human Tumor Cell Cultures." In Methods in Molecular Biology, 201–15. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-854-2_13.

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Greco, Steven J., Shyam A. Patel, and Pranela Rameshwar. "A Reporter Assay to Detect Transfer and Targeting of miRNAs in Stem Cell-Breast Cancer Co-cultures." In Somatic Stem Cells, 195–201. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-815-3_13.

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Meinert, Christoph, Christina Theodoropoulos, Travis J. Klein, Dietmar W. Hutmacher, and Daniela Loessner. "A Method for Prostate and Breast Cancer Cell Spheroid Cultures Using Gelatin Methacryloyl-Based Hydrogels." In Methods in Molecular Biology, 175–94. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7845-8_10.

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Degen, G. H. "Ovine Seminal Vesicle Cell Cultures, A Tool for Studies of Carcinogen Activation by Prostaglandin H Synthase." In Eicosanoids and Other Bioactive Lipids in Cancer, Inflammation and Radiation Injury, 419–21. Boston, MA: Springer US, 1993. http://dx.doi.org/10.1007/978-1-4615-3520-1_82.

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Portelli, Lucas A., Aditya Kausik, and Frank S. Barnes. "Effects of small and rapid temperature oscillations on adherent cell cultures: Exposure system, experimental method and a pilot study on human cancer cells." In EMBEC & NBC 2017, 707–10. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-5122-7_177.

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Conference papers on the topic "Cancer cell cultures"

Irakleidis, Foivos, Michael Masucci, Eleftherios Sfakianakis, Peng H. Tan, and Hazel Welch. "MODIFIED SLOW DIGESTION TECHNIQUE FOR THE ISOLATION OF PATIENT-DERIVED CELLS: AN IN VITRO MODEL FOR THE DESIGN OF BREAST CANCER-ASSOCIATED STROMA." In Abstracts from the Brazilian Breast Cancer Symposium - BBCS 2021. Mastology, 2021. http://dx.doi.org/10.29289/259453942021v31s2018.

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Objective: Highly prevalent cancer-associated fibroblasts (CAFs) are understood to play a key role in tumorigenesis. Understanding of CAFs and tumor-associated stroma is considered to be essential in novel cancer therapies. Patient-derived cells (PDCs) more closely resemble tumor microenvironment compared with commercial cell lines that are subjected to genetic and phenotypic changes. However, PDCs use can be limited by challenges in isolating high-yield viable cultures. Overcoming these challenges would benefit novel personalized cancer research. In this study, we aimed to investigate the effectiveness of modified tissue digestion processing techniques of isolation of PDCs. Methodology: PDCs were isolated from breast tissues collected from patients who had previously been diagnosed with breast cancer. Modification of slow and fast digestion processing techniques was used, followed by analysis for morphology and protein marker expression. Results: Isolated PDCs were presented with different morphologies and functions compared with breast cancer cell lines. Higher growth potential was observed with a combination of maintenance and filtered conditioned medium. High expression of Vimentin and morphological characteristics of spindle-shaped large cells confirmed the PDCs as fibroblasts. The modified slow digestion approach used in this study was successful in isolating fibroblasts from retrieved breast tissue. The fast digestion approach was not viable and was abandoned early due to poor production of cells. Conclusions: PDCs were isolated using a modified slow digestion approach. PDC cultures can more effectively represent breast cancer stroma and are becoming an essential platform for research as a personalized in vitro model for molecular breast cancer research. This study presents a highly successful method of isolating PDCs from breast cancer patients.

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Shim, Youn Young, Timothy Tse, and Martin Reaney. "Biological Activities of Flaxseed Peptides (Linusorbs)." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/zrcc3198.

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Flaxseed (Linum usitatissimum >L.) is gaining popularity in the food industry as a superfood due to its health-promoting properties. The flax plant synthesizes an array of biologically active cyclic peptides or linusorbs (LOs, a.k.a. cyclolinopeptides) from three or more ribosome-derived precursors. [1–9-NαC]-linusorb B3 and [1–9-NαC]-linusorb B2, suppress immunity, induce apoptosis in human epithelial cancer cell line (Calu-3) cells, and inhibit T-cell proliferation, but the mechanism of LOs action is unknown. Using gene expression analysis in nematode cultures and human cancer cell lines we have observed that LOs exert their activity, in part, through induction of apoptosis. Specific LOs’ properties include: 1) distribute throughout the body after flaxseed consumption; 2) induce heat shock protein (HSP) 70A production as an indicator of stress and addressed the issue in Caenorhabditis elegans (exposure of nematode cultures to [1–9-NαC]-linusorb B3 induced a 30% increase in production of the HSP 70A protein); 3) induce apoptosis in Calu-3 cells; and 4) modulate regulatory genes in microarray analysis. These diverse activities indicate that LOs might induce apoptosis in cancer cells or act as versatile platforms to deliver a variety of biologically active molecules for cancer therapy.

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Takayama, Shuichi, Dongeun Huh, Jonathan Song, Wansik Cha, and Yunseok Heo. "Micro- and Nanofluidics for Cell Biology, Cell Therapy, and Cell-Based Drug Testing." In ASME 2009 7th International Conference on Nanochannels, Microchannels, and Minichannels. ASMEDC, 2009. http://dx.doi.org/10.1115/icnmm2009-82151.

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Many biological studies, drug screening methods, and cellular therapies require culture and manipulation of living cells outside of their natural environment in the body. The gap between the cellular microenvironment in vivo and in vitro, however, poses challenges for obtaining physiologically relevant responses from cells used in basic biological studies or drug screens and for drawing out the maximum functional potential from cells used therapeutically. One of the reasons for this gap is because the fluidic environment of mammalian cells in vivo is microscale and dynamic whereas typical in vitro cultures are macroscopic and static. This presentation will give an overview of efforts in our laboratory to develop microfluidic systems that enable spatio-temporal control of both the chemical and fluid mechanical environment of cells. The technologies and methods close the physiology gap to provide biological information otherwise unobtainable and to enhance cellular performance in therapeutic applications. Specific biomedical topics that will be discussed include, in vitro fertilization on a chip, microfluidic tissue engineering of small airway injuries, breast cancer metastasis on a chip, electrochemical biosensors, and development of tuneable nanofluidic systems towards applications in single molecule DNA analysis.

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Saribudak, Aydin, Herman Kucharavy, Karen Hubbard, and M. Umit Uyar. "Quantification of cell apoptosis for in-vitro colorectal cancer cell cultures based on morphological features." In 2016 IEEE-EMBS 3rd International Conference on Biomedical and Health Informatics (BHI). IEEE, 2016. http://dx.doi.org/10.1109/bhi.2016.7455840.

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Nikkhah, Mehdi, Jeannine S. Strobl, Bhanu Peddi, Adedamola Omotosho, and Masoud Agah. "Micropattern Effect on Breast Cancer Cells Behavior on Isotropically Etched Silicon Microenvironments." In ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology. ASMEDC, 2010. http://dx.doi.org/10.1115/nemb2010-13030.

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In this paper we are investigating three dimensional (3-D) silicon-based microenvironments as potential platforms for breast cancer diagnostics. We have developed isotropically etched microstructures with a wide range of geometrical patterns for this purpose. Our results indicate that with the etched surface ratio of ∼65%, it is possible to capture 80–90% of the cancer cells within each silicon chip. After treatment of the cells with mitomycin C (to block the cell growth) more number of the cells are trapped inside the etched features for longer cultures times (72 h) suggesting that there is a directed motility and attraction of the cells toward the etched cavities and by optimally designing the etched features, the proposed platforms can be potentially used for diagnostics purposes.

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Hombrink, Pleun, Soura Mardpour, Ewout Spaan, Jarmil Hanrath, Farzin Pourfarzad, Jasper Mullenders, Rene Overmeer, et al. "Abstract 2977: Organoid-T-cell co-cultures for preclinical testing of adoptive cell and cancer immunotherapy." In Proceedings: AACR Annual Meeting 2021; April 10-15, 2021 and May 17-21, 2021; Philadelphia, PA. American Association for Cancer Research, 2021. http://dx.doi.org/10.1158/1538-7445.am2021-2977.

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Lee, Sheng-Lin, Kenneth M. Pryse, Boyd Butler, Elliot L. Elson, and Guy M. Genin. "Mechanically-Induced Cytoskeletal Remodeling in 3D Cultures of Contractile Fibroblasts." In ASME 2010 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2010. http://dx.doi.org/10.1115/sbc2010-19684.

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Fibroblasts are responsible for collagen biosynthesis and organization in connective tissues, and play a role in regulation of epithelial differentiation, regulation of inflammation, and wound healing. Increasing evidence suggests that fibroblast might promote tumors by facilitating angiogenesis and cancer progression [1]. They are coupled mechanically to their fibrillar extra-cellular matrix, which can regulate cell behavior through its composition and mechanics.

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Cruvinel-Carloni, Adriana, Renato José da Silva-Oliveira, Viviane Aline Oliveira-Silva, Marcela Nunes Rosa, Lucas Tadeu Bidinotto, Gustavo Noriz Berdinarelli, Raul Torrieri, et al. "Abstract A29: Omics profile of two immortalized Brazilian glioblastoma cell cultures." In Abstracts: AACR International Conference held in cooperation with the Latin American Cooperative Oncology Group (LACOG) on Translational Cancer Medicine; May 4-6, 2017; São Paulo, Brazil. American Association for Cancer Research, 2018. http://dx.doi.org/10.1158/1557-3265.tcm17-a29.

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Daszkiewicz, Lidia, Gera Goverse, Nataliia Beztsinna, Kuan Yan, Emma Spanjaard, and Leo Price. "Abstract B137: Visualization and quantification of tumor-immune cell interactions in 3D cultures." In Abstracts: AACR-NCI-EORTC International Conference on Molecular Targets and Cancer Therapeutics; October 26-30, 2019; Boston, MA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1535-7163.targ-19-b137.

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Cydenova, I. A., M. M. Cyganov, M. K. Ibragimova, A. A. Nushtaeva, and N. V. Litvyakov. "MECHANISMS OF ESCAPE FROM REPLICATIVE SENESCENCE OF TUMOUR CELLS AFTER EXPOSURE TO CHEMOTHERAPY." In I International Congress “The Latest Achievements of Medicine, Healthcare, and Health-Saving Technologies”. Kemerovo State University, 2023. http://dx.doi.org/10.21603/-i-ic-147.

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In 25% of patients with breast cancer, tumor cells after neoadjuvant chemotherapy (NAHT) leave the state of replicative aging and form macrometastases. In patients with breast cancer, it was shown that the exit from replicative aging with metastasis is observed only in those patients in whose tumor WNT signaling is ectopically activated due to amplification of activator genes and deletions of negative regulators of this signaling pathway. Purpose: in a direct experiment on cell cultures differing in ectopic activation of WNT signaling due to CNA (Copy Number Aberration) WNT signaling genes, to study the ability of tumor cells to exit replicative senescence after exposure to chemotherapy drugs.

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Reports on the topic "Cancer cell cultures"

Type, P. T. Establishment of a Repository for Cell Cultures and Genomic DNA from Breast Cancer Patients. Fort Belvoir, VA: Defense Technical Information Center, September 1996. http://dx.doi.org/10.21236/ada319903.

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Merkle, Carrie J. Studies on Breast Cancer Cell Interactions with Aged Endothelial Cells in Culture and Rat Models. Fort Belvoir, VA: Defense Technical Information Center, May 2006. http://dx.doi.org/10.21236/ada455981.

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Latimer, Jean J. A New Paradigm for African American Breast Cancer Involving Stem Cell Differentiation in a Novel Cell Culture System. Fort Belvoir, VA: Defense Technical Information Center, October 2006. http://dx.doi.org/10.21236/ada462736.

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Zhao, Yunge. Promoting Breast Cancer Cell Invasion by Matrix Metalloproteinase-26 in Novel Threedimensional PVA Sponge Culture System. Fort Belvoir, VA: Defense Technical Information Center, September 2004. http://dx.doi.org/10.21236/ada437627.

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Hristova, Svetlana H., and Alexandar M. Zhivkov. Cytotoxic Effect of Exogenous Cytochrome C Adsorbed on Montmorillonite Colloid Particles on Colon Cancer Cell Culture. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, February 2019. http://dx.doi.org/10.7546/crabs.2019.02.08.

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Mastro, Andrea M. Altering the Microenvironment to Promote Dormancy of Metastatic Breast Cancer Cell in a 3D Bone Culture System. Fort Belvoir, VA: Defense Technical Information Center, April 2014. http://dx.doi.org/10.21236/ada604844.

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Mastro, Andrea M., and Erwin Vogler. Altering the Microenvironment to Promote Dormancy of Metastatic Breast Cancer Cell in a 3D Bone Culture System. Fort Belvoir, VA: Defense Technical Information Center, April 2015. http://dx.doi.org/10.21236/ada621382.

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Zhao, Yunge. Promoting Breast Cancer Cell Invasion by Matrix Metalloproteinase 26 in a Novel Three-Dimensional PVA Sponge Culture System. Fort Belvoir, VA: Defense Technical Information Center, September 2005. http://dx.doi.org/10.21236/ada448489.

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Allworth, Ann E. The Role of Terbium and Gadolinium in Reversal of Cisplatin Resistance in Cultured Human Breast Cancer Cells. Fort Belvoir, VA: Defense Technical Information Center, June 2001. http://dx.doi.org/10.21236/ada414791.

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