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1
Cavalcante, Dannuza Dias. "Estratégias analíticas para determinação de arsênio e selênio em amostras de alimentos utilizando a espectrometria de fluorescência atômica com geração de hidretos – HG AFS." Instituto de Química, 2014. http://repositorio.ufba.br/ri/handle/ri/16478.
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CNPq
Neste trabalho que está no âmbito do PRONEX, foram desenvolvidas estratégias analíticas para a determinação de arsênio e selênio em amostras de alimentos por HG AFS. Foram realizados três trabalhos distintos. O primeiro consistiu no emprego da amostragem e m suspenção para determinação de arsênio em amostras de arroz. Procedimentos de amostragem de suspensão foram avaliados para determinação de As por geração de hidreto acoplado a AFS, usando HNO 3 e sonicação por 30 min. As amostras foram preparadas com KI e m ácido ascórbico e com HCl 6 mol L - 1 , para determinação As total. A exatidão foi confirmada por análise do material de referência certificado NIES SRM 10b de farinha de arroz, a precisão foi confirma com valores de RSD abaixo de 5,9 % e limites de detecçã o e quantificação de 0,91 e 3,04 ng L - 1 , respectivamente. Este método foi utilizado para determinar o teor de arsênio em 24 amostras de arroz que foram adquiridas em supermercados da cidade de Salvador, Bahia, Brasil. O conteúdo de arsênio nos três tipos de arroz (branco, parbolizado e integral) variou de 0,12 a 0,47 μg g - 1 . O segundo trabalho foi o desenvolvimento de método analítico para determinação de selênio em ovos. Três tipos de ovos foram adquiridos (codorna, galinha e pata) em feiras e supermercad os de Salvador. A digestão foi realizada mediante adição de HNO 3, H 2 O 2 30% v v - 1 e HCl 6 mol L - 1 , utilizando o bloco digestor com dedo frio. As condições para a pré - redução e geração do hidreto de selênio foram otimizadas empregando o planejamento fatorial e a matriz de Doehlert. As condições ótimas foram: concentração de HCL 5,3 mol L - 1 , concentração do borohidreto de sódio 2,6 % (m/v), volume de KBr 10% 1,0 mL e tempo de pré - redução de 30 min. O método apresentou limites de detecção e quantificação de 0,22 e 0,77 ng L - 1 , respectivamente. O RSD ficou abaixo de 4,7 % demonstrando boa repetibilidade. A exatidão foi comprovada através da análise do CRM de tecido de ostra e também através de comparação com resultados obtidos e m análise no ICP - MS. O método foi aplicado em quatro diferentes grupos de amostras, na clara e na gema separadas e na mistura dos dois, sendo que as concentrações mínimas e máximas foram de 0,35 ± 0,01 a 0,88 ± 0,03 μg g - 1 . O terceiro trabalho foi o desenv olvimento de método para determinação de arsênio em atum e sardinha enlatados. As amostras foram submetidas a 3 procedimentos de preparo de amostra (bloco digestor, forno de micro - ondas e forno mufla). As condições para a pré - redução e geração do hidreto d e arsênio foram otimizadas empregando o planejamento fatorial e a matrix de Doehlert e as condições encontradas foram: tempo de pré - redução de 21 min, volume de pré - redutor KI 10 % (m v - 1 ) em ácido ascórbico 2% (m v - 1 ) de 1,0 mL, concentração de HCl 4,7 mo l L - 1 e concentração de NaBH 4 de 2% (m v - 1 ). O método mostrou - se preciso, com valores de RSD abaixo de 7,0 %. Um material de referência certificado de tecido de ostra (NIST SRM 1566b) foi analisado para avaliar a exatidão do método. O material foi submetid o a três procedimentos de digestão. Através da análise dos resultados pode - se observar que o valor obtido no forno de micro - ondas e no bloco digestor foi cerca de metade do valor certificado, pois a arsenobetaina só é convertida a arsênio inorgânico a temp eraturas acima de 300 º C. O método foi aplicado para 20 amostras de atum e sardinha enlatados e os valores de concentração variaram de: 0,63 ± 0,10 a 3,28 ± 0,20 μg g - 1
In this work is under PRONEX , strategies for analytical determination of arsenic and selenium in food samples by HG AFS were developed. Three diferent studies were conducted. The first one consisted in the use of sampling in suspension for determination of arsenic in rice procedures were evaluated to As by hydride generation coupled to AFS using HNO 3 and sonication for 30 min. The samples were prepared with KI in ascorbic acid and 6 mol L - 1 HCl to determine total As . The accuracy was confirmed by analysis of certified reference material NIES SRM 10b rice flour , precision was confirmed with % RSD values lower than 5.9% and limits of detection and quantification of 0. 91 and 3.04 ng L - 1 , respectively. This method was used t o determine the content of arsenic in 24 rice samples purchased at supermarkets in the city of Salvador , Bahia , Brazil . The content of arsenic in the three types of rice ( white, parboiled and integral ) ranged from 0.12 to 0.47 μ g g - 1 . In the second study we developed a method for determination of selenium in eggs. Three types of eggs were purchased ( quail, chicken and paw) at fairs and supermarkets of Salvador . The digestion was performed by adding HNO 3 , 30% H 2 O 2 ( v v - 1 ) , 6 mol L - 1 HCI and , using the block digester cold finger. The conditions for the pre - reduction and hydride generation of selenium were optimized using factorial design and Doehlert matrix, the optimal conditions were : HCl concentration of 5.3 mol L - 1 , the 2.6 % ( w v - 1 ) sodium borohydride concentration , 1.0 mL volume of 10% ( w v - 1 ) KBr and pre - reduction time 30 min. The method has limits of detection and quantification of 0.2 2 and 0.7 7 ng L - 1 , respectively. The % RSD was below 4.7 showing good repeatability . The accuracy was confirmed by analysis of CRM oyster tissue and also by comparison with results obtained for analysis by ICP - MS. The method was applied to four different groups of samples , in clear and separate yolk and mix of the two , the minimum and maximum concentrations were 0.35 ± 0.01 to 0.88 ± 0.03 mg g - 1 . The third work was the development of a method for determination of arsenic in canned tuna and sardines . The samples were subjected to three procedures for sample preparation ( digestion block, microwave and oven muffle ). The co nditions for the pre - reduction and hydride generation of arsenic were optimized using factorial design matrix and Doehlert , the conditions were: pre - reduction time of 21 min, volume of 1.0 mL of 10% KI ( w v - 1 ) (pre - reducing) in 2% a scorbic acid, HCl concentration of 4.7 mol L - 1 and NaBH 4 - in 2% ( v m - 1 ). The method was precise, with RSD values below 7.0 %. A certified reference material of oyster tissue ( NIST SRM 1566b ) was analyzed to assess the accuracy of the method . The material was subjected to the three digestion procedure. From t he analysis of the results it can be seen that the value obtained in the microwave oven and the digester block was about half the value of the certificate , as the only arsenobetaine is converted to inorganic arsenic at temperatures above 300 °C. The method was applied to 20 samples of canned tuna and sardines and concentration values ranged from 0.63 ± 0.1 0 to 3.28 ± 0.2 0 mg g - 1
2
Asadi, Khalid. "The potential cancer preventative properties of sweetpotato anthocyanin-rich polyphenolic complex." Thesis, University of Auckland, 2006. http://hdl.handle.net/2292/3142.
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Anthocyanins are a widespread source of naturally occurring colorants distributed in fruits, vegetables, and beverages such as red wine. Anthocyanin-rich foods and preparations have been reported to reduce the risk of life-style related diseases, including reducing the risk of coronary heart disease, preventing some chronic inflammatory diseases, protecting against cancer and slowing the ageing process. SL222 sweetpotato, a purple-fleshed cultivar, accumulates high levels of anthocyanins in the storage-root. This project examines the chemopreventative properties of the SL222 sweetpotato in the C57BL/6J-APCMIN /+Apc (APCMIN ) mouse, a genetic model of colorectal cancer, and the potential underlying mechanisms both in tissue culture and in vivo.Thesis under embargo until April 2009. Available by individual request after embargo expires, use the feedback form to request access.
3
Alsouhibani, Norah. "AU-rich mRNA expression in relation to breast cancer-related processes." Thesis, University of Newcastle Upon Tyne, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.525033.
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Dyer, Karen Elizabeth. "Survivorship, Infertility and Parenthood: Experiencing Life after Cancer in Puerto Rico." Scholar Commons, 2013. http://scholarcommons.usf.edu/etd/4475.
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While incidence rates are increasing for many cancers in Puerto Rico, mortality rates are declining (Torres-Cintron, et al. 2010), resulting in growing numbers of survivors and creating a situation in which long-term survivorship concerns are beginning to emerge as priorities. The importance of quality-of-life among survivors of cancer is increasingly being recognized among healthcare providers, although there remains a gap in knowledge of how young adult survivors cope with long-term treatment-related physical effects, such as infertility, and of the impact of cancer on survivors' social relationships and future goals.
Because understandings of "cancer survivorship," as well as of reproduction, vary according to cultural context, this study examined the physical and social impact of cancer on young adults in Puerto Rico, and specifically the importance of parenthood. A media analysis of women's magazines, key informant interviews with ten cancer researchers, as well as in-depth, semi-structured interviews with 23 young adult cancer survivors, 16 healthcare providers, nine cancer advocates, and two members of the clergy were conducted in order to shed light on the lived experiences, needs, and concerns of young Puerto Rican cancer survivors.
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DeNicola, Cafferky Katie. "ASSESSMENT OF METAL CONCENTRATIONS AND SPECIES IN BIOLOGICALLY RELEVANT SAMPLES WITH INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRIC TECHNIQUES." University of Cincinnati / OhioLINK, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1155754956.
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Geng, Wei, and 耿瑋. "The role of proline rich tyrosine kinase 2 (Pyk2) on cisplatin resistance in HCC." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B43224106.
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Ruhland, Sarah L. "The fragility of significance the rise and fall of the Cannon Ball House /." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 47 p, 2007. http://proquest.umi.com/pqdweb?did=1338866231&sid=9&Fmt=2&clientId=8331&RQT=309&VName=PQD.
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Watson, Julie Karen. "The roles of the small leucine-rich repeat proteoglycans, osteomodulin and PRELP, in urothelial cell carcinoma." Thesis, University of Cambridge, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.608742.
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Morris, Cathryn. "Miracles or myth : the royal Raymond Rife story." Honors in the Major Thesis, University of Central Florida, 2001. http://digital.library.ucf.edu/cdm/ref/collection/ETH/id/236.
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This item is only available in print in the UCF Libraries. If this is your Honors Thesis, you can help us make it available online for use by researchers around the world by following the instructions on the distribution consent form at http://library.ucf.edu/Systems/DigitalInitiatives/DigitalCollections/InternetDistributionConsentAgreementForm.pdf You may also contact the project coordinator, Kerri Bottorff, at kerri.bottorff@ucf.edu for more information.Bachelors
Arts and Sciences
Film
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Kayawake, Hidenao. "Protective Effects of a Hydrogen-Rich Preservation Solution in a Canine Lung Transplantation Model." Doctoral thesis, Kyoto University, 2021. http://hdl.handle.net/2433/263568.
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Thulin, Åsa. "The Role of Histidine-rich Glycoprotein in Angiogenesis and Tumor Growth." Doctoral thesis, Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-110829.
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Histidine-rich glycoprotein (HRG) is a heparin-binding plasma protein modulating immune, hemostatic and vascular functions. I have studied the antiangiogenic functions of HRG in vitro and in vivo in order to understand the molecular mechanisms of action of HRG as an angiogenesis inhibitor. Angiogenesis is the formation of new blood vessels from the pre-existing vasculature. It is a central rate-limiting step of tumor development and thus a possible target for cancer therapeutics. Previous studies have shown that HRG has antiangiogenic functions in vivo and that the antiangiogenic effects are mediated via the proteolytically released His/Pro-rich domain of HRG. In this thesis we demonstrate that HRG can inhibit endothelial cell migration by interfering with focal adhesion and cytoskeletal turnover. Moreover we have identified the minimal active domain of HRG, a 35 amino acid peptide derived from the histidine- and proline-rich domain of HRG. Analyzing human tumor tissue samples, we have found that a His/Pro-rich fragment of HRG is bound to the vasculature from cancer patients but not to the vasculature from healthy individuals. The fragment is found in association with platelets, and we show that activated platelets can induce a functional microenvironment for the His/Pro-rich fragment. Cancer patients often display an increased coagulation and our data describe a new mechanism to confer specificity of an angiogenesis inhibitor for situations with enhanced platelet activation, as in the tumor. We have further studied the role of HRG in tumor growth by crossing HRG-deficient mice with a transgenic mouse model of pancreatic insulinoma. We show that mice lacking HRG display an elevated “angiogenic switch” and that the total tumor volume is larger in these mice than in wild type mice. HRG is also involved in regulation of platelet function and platelets can stimulate angiogenesis in various ways. We have depleted mice of platelets to study the possible connection between the function of HRG in angiogenesis and platelet regulation. Our data suggest an involvement of platelets in the antiangiogenic activities of HRG.
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Papadaki, V. "Roles of the small leucine-rich repeat proteoglycans OMD and PRELP in development and cancer." Thesis, University College London (University of London), 2014. http://discovery.ucl.ac.uk/1460134/.
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Osteomodulin (OMD) and Proline/arginine-rich and Leucine-rich Repeat protein (PRELP) belong to the small leucine-rich repeat proteoglycan (SLRP) family and as extracellular matrix components have the ability to influence various cellular functions, including cell growth, migration and proliferation, while their mutation or aberrant expression can cause developmental disorders and cancer. This thesis extends previous work in further understanding the roles of OMD and PRELP in cancer and also during mouse development. In the current project OMD and PRELP are overexpressed in a bladder cancer cell line where they are found to alter cell morphology, reduce cell invasion and anchorage-independent growth, while they also inhibit tumour growth in xenograft mouse models. Additionally, we show that OMD and PRELP mediate their effects by cross-regulating different signalling pathways and also by affecting tight junction formation. Furthermore, novel knock-out mouse models of OMD and PRELP were generated, where a Lac-Z cassette has been inserted in the coding regions of the two genes, allowing us to follow their expression under X-gal staining. Therefore a detailed analysis of expression patterns was initially conducted. OMD and PRELP were both expressed in skeletal elements during mouse development, while in adult mice they were differentially expressed in neurons and epithelia of the brain and the eye, and in the urothelium of the bladder. Finally, the novel knock-out mice were used to assess any cancer-related aberrant phenotypes arising from OMD and PRELP deficiency. The bladders of the knock-out mice presented with early stages of urothelial papillary formations, where the junctional complexes were also disrupted, suggesting that lack of OMD and/or PRELP is permissive for cancer initiation. Overall, with our current findings we hope to improve the understanding of SLRP biology in carcinogenesis, and we would like to propose OMD and PRELP as potential targets for the development of cancer therapies.
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Geng, Wei. "The role of proline rich tyrosine kinase 2 (Pyk2) on cisplatin resistance in HCC." Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B43224106.
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Sun, Kin-wai, and 孫建維. "The significance of proline rich tyrosine kinase 2 (PYK2) in proliferation and invasiveness of hepatocellular carcinoma." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2008. http://hub.hku.hk/bib/B40687375.
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Ventrucci, Gislaine. "Cancer-caquexia e suplementação nutricional : impacto da dieta rica em leucina no controle do metabolismo proteico muscular." [s.n.], 2005. http://repositorio.unicamp.br/jspui/handle/REPOSIP/314505.
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Orientador: Maria Cristina Cintra Gomes MarcondesTese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: Caquexia, presente na maioria dos hospedeiros com câncer, é um estado caracterizado pela perda involuntária de peso. Pacientes com caquexia apresentam expectativa de vida muito reduzida e menor qualidade de vida. Nos pacientes com câncer-caquexia há intensa mobilização de substratos dos tecidos da carcaça do organismo hospedeiro ocorrendo, preferencialmente, depleção de proteína muscular em função da redução da síntese e/ou aumento da degradação protéica no músculo. Este aumento da taxa de proteólise muscular tem como função prover aminoácidos para síntese de glutamina, bem como para a demanda de síntese das células neoplásicas. Trabalhos da literatura mostram que o desenvolvimento de câncer dá-se de forma mais agressiva e severa quanto mais jovem for o paciente. Pacientes acometidos pelo crescimento de neoplasia maligna concomitante à gravidez sofrem da mesma agressividade desta doença, com um agravante maior tratam se de dois pacientes: mãe e feto. Neste trabalho analisamos os efeitos de uma dieta rica em leucina sobre o metabolismo protéico em animais jovens prenhes portadores ou não do carcinossarcoma de Walker 256. Ratas Wistar foram distribuídas em grupos experimentais de acordo com a inoculação ou não do carcinossarcoma de Walker 256 e submetidas ou não a dieta rica em leucina. Após 20 dias de experimento foi realizado ensaios com o músculo esquelético (gastrocnêmio) a fim de elucidar o mecanismo de catabolismo tecidual que ocorre durante o processo de câncer-caquexia. Os grupos apresentaram aumento da taxa de proteólise muscular e redução da taxa de síntese protéica muscular. Porém, o grupo inoculado com tumor e tratado com dieta rica em leucina apresentou aumento da síntese protéica e menor espoliação de proteína muscular quando comparado com o grupo inoculado com tumor e não tratado com leucina na dieta. A suplementação de leucina na dieta, uma vez que este aminoácido é utilizado como fonte energética pelo músculo esquelético pode prevenir a depleção da carcaça, preservar a massa protéica corpórea e impedir o estado caquético do animal
Doutorado
Fisiologia
Doutor em Biologia Funcional e Molecular
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Li, Gang. "Generation of Tumor-Specific Immunity Using HER2/NEU Positive Tumor Derived Chaperone-Rich Cell Lysate (CRCL)." Diss., The University of Arizona, 2007. http://hdl.handle.net/10150/193817.
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HER2/neu is an oncogenic tumor-associated antigen over-expressed in several human tumors including breast and ovarian cancer. The selective expression of HER2/neu and its role in epithelial carcinogenesis makes HER2/neu an ideal target for immunotherapy. Tumor-derived chaperone-rich cell lysate (CRCL), containing numerous heat shock proteins, has successfully been used to generate tumor-specific immunity against a wide range of murine tumors and is a great candidate for an effective vaccine against HER2/neu positive tumors. In the first part of this study, the potency of human ovarian cancer-derived CRCL to activate dendritic cells (DCs) and to generate tumor-specific T cells in vitro has been investigated. Chaperone-rich cell lysate was generated from primary ovarian cancer tissues and SKOV3-A2, a HER2/neu, Wilm's tumor gene 1 (WT1) and HLA-A2 positive human ovarian tumor cell line. T cells from healthy donors and from ovarian cancer patients secreted higher amounts of interferon-γ following in vitro re-stimulation with ovarian cancer-derived CRCL compared to HER2/neu or WT1 peptide-pulsed DCs. We were also able to generate cytotoxic T lymphocyte activity against cancer-specific antigens such as HER2/neu and WT1 from all healthy donors, but from only one of the four ovarian cancer patients with bulky disease. In the second part of the study, the potency of tumor-derived CRCL to elicit the humoral immune response against a murine HER2/neu positive tumor (TUBO) has been examined. Vaccination of mice bearing a palpable tumor efficiently delayed the development of the tumor. In the vaccinated mice, CRCL vaccination induced significant anti-HER2/neu antibodies. Using B cell deficient mice and antibody transfer experiments, we have shown that the induction of anti-HER2/neu antibodies is both necessary and sufficient for the anti-tumor effect. Further, we have demonstrated that serum from TUB0 CRCL-vaccinated mice stimulated the internalization of the HER2/neu molecules, resulting in the down-regulation of their surface expression. Moreover, antibody-dependent cellular cytotoxicity has been observed against TUBO cells when presented with sera from vaccinated mice. These results indicate that CRCL may be a potent adjuvant for women suffering from HER2/neu positive ovarian or breast cancer and that this personalized vaccine may be a promising approach for active immunotherapy.
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Glagolenko, Anna Anatolievna. "Effect of HZE radiation and diets rich in fiber and n-3 poly unsaturated fatty acids (n-3 PUFA) on colon cancer in rats." Texas A&M University, 2005. http://hdl.handle.net/1969.1/3942.
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This study examines the carcinogenic effect of HZE radiation and protective
effects of different types of diets against colon carcinogenesis in a rat model.
The effect of HZE radiation on health state and colon cancer development was
evaluated. HZE radiation was found to suppress food consumption (P<0.0001) leading to
lower body weight gain of irradiated rats when compared to the non-irradiated rats
(P<0.05). The animals exposed to HZE radiation were found to start dying and/or getting
pathologies 11 weeks earlier and at the end of the study had morbidity/mortality rate
14.2% higher (P=0.0005) than non-irradiated rats. There was no significant effect of
HZE radiation on colon cancer incidence.
The effects of dietary fibers and oils on health state and colon carcinogenesis
were evaluated. Morbidity/mortality was found to be delayed in rats fed with pectinbased
diets when compared to cellulose-based diet, regardless of radiation treatment.
Similarly, fish oil was found to beneficially affect health of the experimental animals
when compared to corn oil. Ten- and twenty-week delayed morbidity/mortality for
irradiated and non-irradiated groups, respectively, was observed for rats fed with fish
oil-based diets when compared to corn oil-based diets. Fish oil was also found to
significantly reduce colon tumor incidence and multiplicity in non-irradiated rats
(P<0.05). A similar trend was observed for the irradiated animals. No significant effect
of fiber on colon cancer incidence was found.
Finally, the effect of diets on general health and colon cancer development was
investigated. Rats fed with corn oil/cellulose diet started dying and/or getting a disease
earlier than rats fed with other diets, regardless of radiation treatment. The effect of diet
on colon cancer development was found to depend on radiation treatment. Thus, in the
absence of radiation treatment fish oil/cellulose was found to significantly reduce tumor
incidence and multiplicity when compared to corn oil/pectin diet (P<0.05). In the
presence of radiation treatment fish oil/pectin was found to lower the values of tumor
incidence and tumor multiplicity, though the data obtained were not significant.
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Pereira, Lucy. "Validation of the 60-second chair rise as a measure of physical function in patients with non-small cell lung cancer." Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=116113.
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Yearly, 22, 200 Canadians are diagnosed with lung cancer, with 80-85% of the cases being non-small cell lung cancer (NSCLC). With diagnoses being predominantly in the advanced stages, prognosis is poor and quality of life (QoL) becomes the focus of treatment. The main symptom cachexia, issues a loss of strength and impacts on an important aspect of QoL, physical function. Physical function is predominately assessed subjectively. Lately performance-based measures are gaining in popularity. One performance measure, the chair rise test, has not been validated in the NSCLC population and was the objective of this study.Subjects completed the chair rise test, 6MWT, hand grip, and the SF-36 pre and post chemotherapy. Evidence for construct and discriminant validity but not predictive validity was provided for the chair rise test. The 60-second chair rise may be too strenuous for persons with severe disability but a standardized timed-based chair rise test is needed.
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Sitthiphong, Piyawan. "Impact of flavonoid-rich and flavonoid-poor fruit and vegetables on biomarkers of cancer risk in a human randomized controlled trial." Thesis, University of Reading, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.602467.
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Epidemiological studies have shown an association between fruit and vegetable (F&V) consumption and decreased risk of cancer at several sites. However, there are few human intervention studies that provide information about a causal link between F&V consumption and cancer risk and about the optimum amounts of F&V that need to be consumed and whether some types of F&V are more effective than others. This study aims to investigate the impact of increasing intake of flavonoid-rich and flavonoid-poor F&V in human subjects on three endpoints: lymphocyte DNA damage, inflammation markers and gene expression. Blood samples were collected from a randomised, controlled, parallel design study in 80 volunteers divided into three study groups. The participants in the two test groups: high flavonoid F&V (HF) and low flavonoid F&V (LF) were asked to increase F&V intake by 2, 4 and 6 portions/day for duration of 6 weeks each while subjects in the control group (CT) maintained their habitual diet. Increasing F&V intake by 4 and 6 portions/day reduced the endogenous oxidation of DNA bases in the LF group and increasing F&V intake at 6 portions/day enhanced the ability of lymphocytes to resist to exogenously induced ( by H,O,) DNA damage in both test groups. Increasing F&V consumption may reduce chronic inflammation as a consequence of a decrease in the expression of LFA-l in T lymphocytes by HF group at 4 or 6 portions of intake and an increase in the frequency of CCR-9+ on T cells at 6 portions/day when combining HF and LF data. Furthermore, increasing the intake of F&V of either high or low in flavonoids, modulated the expression of gene transcripts involved in cellular defence. In conclusion, increasing of F&V, either HF or LF, can improve cancer risk biomarkers when compared to CT group. However, flavonoid-rich F&V have no additional benefit compared to flavonoid-poor F&V. Moreover, most effects are found at additional amounts of F&V of 6 portions a day.
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VIDAL, JUNIOR Andr? William Masseaux. "Compara??o entre protocolos para obten??o de plasma rico em plaquetas em c?es: estudo celular." Universidade Federal Rural do Rio de Janeiro, 2017. https://tede.ufrrj.br/jspui/handle/jspui/2444.
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It was proposed by this study to evaluate two protocols (PA and PB) to obtain autologous canine PRP, which is easy to perform in an ambulatory (semiautomatic method) and of good quality (platelet concentration, qualitative evaluation of platelet morphology and low contamination with Erythrocytes), to later propose different therapeutic indications of these PRPs as tissue modulating agents, according to the observed cellular leukocyte pattern. For this purpose, 20 dogs (Canis lupus familiaris) were used at the UFRRJ (HV) Veterinary Hospital, males and females, aged between 1 and 7 years (mean 4 years), considered clinically and hematologically healthy for elective surgeries and / or routine consultations. After adequate trichotomy and antisepsis, 8 mL of blood were collected by venipuncture of the jugular, being immediately packed in two 4 mL flasks, vacuntainer type, containing sodium citrate 3,2%. Protocol A using double centrifugation with 210 xG and 370 xG and protocol B using double centrifugation with 140 x G and 330 x G. PRP samples obtained from each protocol were used to count platelets, erythrocytes and leukocytes in the Neubauer chamber, differential leukocyte counting and observation of platelet morphology in smears. Data (mean and standard deviations) were analyzed by the 95% probability t test (p <0,05) using Pearson's correlation to test the relationship between platelet and erythrocyte counts, platelets and leukocytes and leukocytes in Relation to red blood cells. There was a very weak negative correlation between platelets and leukocytes (? = -0,03), weak negative between platelets and erythrocytes (? = -0,3) and strong positive correlation between leukocytes and erythrocytes (? = 0,75). Although Protocol B did not reach the desired one million platelets average (979300 ? 79631 cells / ?L), both protocols, A and B (4,42 ? 1,61 and 3,85 ? 1,55 times more platelets than Total blood, respectively) (p <0,05) were efficient in concentrating platelets. The cytoplasmic prolongations evidencing platelet activation were present in 26,55 ? 6,72% of platelets of protocol A and 26,25 ? 7,03% in those of protocol B (p> 0,05). A and B presented a small number of red blood cells (p> 0,05), which were considered to be contaminants of the samples and, for the quantity of leukocytes, protocol A presented more white blood cells (p <0,05) than protocol B with higher concentrations of basophils , and lymphocytes.
Foi proposto por esse estudo avaliar dois protocolos (PA e PB) para obten??o de PRP canino aut?logo, de f?cil execu??o em ambulat?rio (m?todo semiautom?tico) e de boa qualidade (capacidade de concentra??o de plaquetas, avalia??o qualitativa da morfologia das plaquetas e reduzida contamina??o com eritr?citos), para posteriormente propor diferentes indica??es terap?uticas desses PRP como agentes moduladores de recupera??o tecidual, de acordo com o padr?o celular leucocit?rio observado. Para isso foram utilizados 20 c?es (Canis lupus familiaris) atendidos no Hospital Veterin?rio da UFRRJ (HV), machos e f?meas, com idade variando entre 1 e 7 anos (m?dia 4 anos), considerados saud?veis clinica e hematologicamente que se destinavam para cirurgias eletivas e/ou consultas de rotina. Ap?s tricotomia e antissepsia adequadas, eram coletados 8 mL de sangue por venopun??o da jugular sendo imediatamente acondicionados em dois frascos de 4 mL, do tipo vacuntainer, contendo citrato de s?dio a 3,2%. O protocolo A utilizando centrifuga??o dupla com 210 xG e 370 xG e protocolo B utilizando centrifuga??o dupla com 140 xG e 330 xG. Amostras de PRP obtidas a partir de cada protocolo foram destinadas a contagem de plaquetas, hem?cias e leuc?citos em c?mara de Neubauer, contagem diferencial dos leuc?citos e observa??o da morfologia das plaquetas em esfrega?os. Analisou-se os dados (m?dias e desvios padr?o) pelo Teste t com 95% de probabilidade (p<0,05) utilizando-se correla??o de Pearson para testar a rela??o entre a contagem de plaquetas e hem?cias, plaquetas e leuc?citos e leuc?citos em rela??o as hem?cias. Houve correla??o negativa muito fraca entre plaquetas e leuc?citos (?= -0,03), negativa fraca entre plaquetas e hem?cias (?= -0,3) e correla??o positiva forte entre leuc?citos e hem?cias (?=0,75). Embora o protocolo B n?o tenha alcan?ando a m?dia um milh?o de plaquetas desejado (979300 ? 79631 c?lulas/?L), ambos os protocolos, A e B (4,42 ? 1,61 e 3,85 ? 1,55 vezes mais plaquetas que o sangue total, respectivamente) (p<0,05) foram eficientes em concentrar plaquetas. Os prolongamentos citoplasm?ticos evidenciando ativa??o plaquet?ria estiveram presentes em 26,55 ? 6,72 % das plaquetas do protocolo A e 26,25 ? 7,03 % nas do protocolo B (p>0,05). PA e PB apresentaram reduzido n?mero de hem?cias (p>0,05) consideradas contaminantes das amostras e, quanto a quantidade de leuc?citos, o protocolo A apresentou mais gl?bulos brancos (p<0,05) que o protocolo B com maiores concentra??es de bas?filos, segmentados e linf?citos.
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Viana, Laís Rosa 1988. "Avaliação do perfil de aminoácidos fetal e materno e atividade placentária em camudongas NMRI portadoras do adenocarcinoma de colon (MAC16) submetidas com dieta rica em leucina." [s.n.], 2012. http://repositorio.unicamp.br/jspui/handle/REPOSIP/314497.
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Orientador: Maria Cristina Cintra Gomes MarcondesDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: A gravidez envolve várias etapas de ajustes fisiológicos, sendo que algumas complicações clínicas podem surgir ao longo deste processo. Dentre as complicações durante a gestação, o câncer destaca-se mais pela sua coexistência com essa complexa condição metabólica, do que pela sua incidência. Na mãe portadora de doença neoplásica, pode haver competição nutricional entre feto e tumor, resultando em alterações no crescimento e metabolismo de ambos. A manipulação da dieta e suplementação nutricional podem minimizar os danos causados pelo tumor. A leucina é um aminoácido que participa do processo de síntese protéica tecidual de forma estrutural e também atua diretamente na sinalização celular; considerando organismos portadores de câncer, a leucina age inibindo o processo de proteólise estimulando o processo de proteogênese. O objetivo desse trabalho foi avaliar o crescimento fetal em associação ao desenvolvimento de câncer em camundongas prenhes submetidas à dieta rica em leucina. Os resultados obtidos referem-se a avaliação de fêmeas adultas (60-90 dias) da linhagem NMRI, prenhes, submetidas a suplementação nutricional com leucina portadoras ou não do adenocarcinoma de colon MAC16 e inoculadas ou não com líquido ascítico ativo ou inativado . Foram avaliados, nessas fêmeas, dados morfométricos como peso relativo de coração, fígado, baço, adrenal, músculo e placenta, sendo observado discreta diminuição do peso relativo da carcaça, coração, músculo e placenta nos grupos portadores de tumor e inoculados com líquido ascítico ativo, quando comparados aos seus grupos controles. Essa diminuição, provavelmente foi induzida pelo crescimento tumoral ou por seus fatores presentes no líquido ascítico. Em contrapartida, houve aumento no peso relativo do fígado e baço nos grupos portadores de tumor, inoculados com líquido ascítico ativo bem como inativo. Também foi possível observar que, nos grupos suplementados com leucina, o peso relativo de alguns órgãos como, músculo e placenta, foi superior ao dos grupos que receberam a dieta controle, mostrando possível efeito protetor da leucina contra a espoliação desses tecidos. Foram analisados também dados bioquímicos nos soros materno e fetal, além da composição corpórea fetal. No soro materno observamos que houve diminuição discreta na concentração de albumina e glicose nos grupos portadores de tumor. Houve aumento da reabsorção de fetos por fêmea nos grupos portadores de tumor e inoculados com líquido ascítico, e ainda nesses mesmos grupos houve modulação desse efeito quando houve suplementação nutricional com leucina. No soro fetal, houve diminuição na concentração de proteínas totais, albumina e glicose, alem do aumento dos aminoácidos gliconeogênicos, como alanina e glutamina, nos grupos implantados com o tumor submetidos a dieta controle. Em contrapartida, os grupos com leucina mostraram discreto efeito protetor da suplementação com esse aminoácido. Concluímos, que os efeitos do crescimento tumoral são para alguns parâmetros mimetizados com a inoculação de líquido ascítico, porém podem ser modulados, na maioria dos parâmetros, com a suplementação nutricional de leucina. A suplementação nutricional com a leucina promoveu efeito benéfico, contribuindo para a manutenção e modulação dos efeitos deletérios causados pela presença da neoplasia, como manutenção da glicemia e proteína totais séricas, além de diminuir reabsorções fetais e também contribuiu para melhorar a atividade placentária nas mães, independente da inoculação ou não do líquido ascítico
Abstract: Pregnancy is a complex process involving several physiological steps and some clinical complications can alter the homeostasis adjustments during this process. Among the complications during pregnancy, cancer is very important for its coexistence than the incidence with this complex metabolic condition. In tumour-bearing mother, there is possible nutritional competition between foetal and tumour, resulting impaired growth and metabolic changes in both mother and foetus. The nutritional supplementation can minimize the damage caused by the tumour. Leucine acts as a cell signalling improving protein synthesis process and tissue structure. Considering cancer patients, leucine inhibits the process of proteolysis. The aim of this study was to evaluate foetal growth in association with cancer development in pregnant mice subjected to leucine-rich diet. We evaluated NMRI pregnant mice (60-90 days-old) feeding control or leucine-rich diet, bearing or not MAC16 colon adenocarcinoma and inoculated or not with active- or inactivated-ascitic fluid. Morphometric data showed decrease in the relative weight of carcass, heart, muscle and placenta in tumour-bearing groups and active-ascitic fluid injected groups. These results may be induced by tumour growth or its factors presented in ascitic fluid. In contrast, we observed increase in relative liver and spleen in tumour-bearing and both active or inactivated ascitic-fluid-inoculated groups. In groups supplemented with leucine, the muscle and placenta relative weight increased in comparison to control diet group, suggesting a possible protective effect of leucine against these tissues wasting. Biochemical data were also analyzed in maternal and foetal serum, and foetal body composition. Maternal serum showed slight decrease in serum albumin and glucose in tumour-bearing groups and also increase in gluconeogenic amino acids, such as glutamine and alanine. The foetuses resorption per female increased in all tumour-bearing groups and ascitic-fluid-inoculated dams. The foetal body water was increased in tumour-bearing animals, and also enhanced the serum pro-inflammatory cytokines and glucagon. We conclude that some effects produced by tumour growth can be similar by the ascitic fluid injection, and can be partially modulated by leucine-rich diet. Nutritional supplementation with leucine promoted beneficial effect, contributing to the maintenance and modulation of the deleterious effects caused by the presence of cancer. These effects can be related to maintenance of blood glucose and serum total protein, and reduction of fetal resorption and also improved the signalling proteins activity in placenta tissue, independent of the inoculation of the ascitic fluid
Mestrado
Fisiologia
Mestre em Biologia Funcional e Molecular
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Gao, Jingfang. "Molecular and Biological Characteristics of Stroma and Tumor Cells in Colorectal Cancer." Doctoral thesis, Linköping : Univ, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-10516.
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Smith, Bruce H., and n/a. "Without motion there cannot be any life : the rise & fall of the 1889 Railway Commissioners : railway management & colonial politics in nineteenth century New Zealand." University of Otago. Department of History, 2007. http://adt.otago.ac.nz./public/adt-NZDU20070619.154352.
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In the nineteenth century, the steam railway became, for many people, the superior conduit for the inland translocation of people and freight. Once devised, steam railways offered such a huge improvement on previous modes and made such a dramatic change to the unity, organisation and commerce of most countries that almost everyone wanted one. New Zealand proved no different, but was faced with not only the twin problems of low population and often rugged geography, but also serious economic problems from difficult world trading conditions and a debt greatly increased by railway construction costs. In the later 1880s, a conservative government decided to vest the Government Railways in independent Commissioners to try to improve productivity and cut out political influence, corruption and jobbery in the huge commercial presence the colony�s railways represented. While this move was successful, a change to one-man-one-vote, together with the pivotal 1890 Maritime Strike, saw the country move left in the elections of 1890, bringing to power a Liberal Government. This new Ministry then set out to reduce the autonomy of the Railway Commissioners, taking four years to return the management of Railways to the direct control of the Government.
While interesting in itself, this is part of the story of the process of the democratic development of New Zealand. This was a community struggling with the often conflicting demands of using railways to not only service the railway debt but also fulfil public transit requirements, including encouraging settlement and economic growth. The organisation�s monopolistic nature and great economic presence, however, offered multiple, including corrupt, opportunities to support the political aspirations of those in power, while offering a less than wonderful service to its customers. Taking place against a backdrop of agitation for railway reform, particularly orchestrated by railway activist Samuel Vaile, the outcome can be seen to have been less than completely desirable for the economic development of the country or its people. This was despite huge support for the principal activist against the Railway Commissioners, Liberal Premier Richard Seddon.
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Souza, Talita Floering Brêda. "Diferenciação osteogênica de células mesenquimais do cordão umbilical canino /." Araçatuba :, 2013. http://hdl.handle.net/11449/121939.
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Resumo:o presente trabalho teve por objetivo isolar células mesenquimais da geléia de Wharton do cordão umbilical canino e avaliar, in vitro, o efeito do plasma rico em plaquetas (PRP) e da matriz óssea desmineralizada (MOD) na diferenciação osteogênica destas células na ausência de meio osteoindutor. As células foram isoladas e caracterizadas como células mesenquimais indiferenciadas com fenótipo semelhante a de fibroblastos e fenótipo característico confirmado pela citometria de fluxo com CD44+, CD105+, CD271+, CD34- e CD45-; além da habilidade de diferenciação em adipócitos, condrócitos e osteócitos. Com exceção do grupo controle (células mesenquimais), em todos os grupos, G-PRP (células mesenquimais e PRP), G-MOD (células mesenquimais e MOD) e G-PRP+MOD (células mesenquimais e PRP + MOD), houve diferenciação osteoblástica com produção de matriz óssea mineralizada, confirmada pela coloração vermelho de Alizarina. A dosagem de fosfatase alcalina (FA) aumentou aos 14 dias em todos os grupos, com redução temporal subsequente. Proteínas ósseas foram expressas na reação em cadeia da polimerase em tempo real e puderam confirmar a diferenciação óssea em todos os grupos. A osteopontina teve sua maior expressão no G-PRP aos sete dias, seguido do grupo G-PRP+MOD... (Resumo completo, clicar acesso eletrônico abaixo)Abstract:This study aimed to isolate MSCs from canine Wharton's jelly umbilical cord and evaluate in vitro the effect of PRP and the MOD in osteogenic differentiation of these cells in the absence of osteogenic medium. Cells were isolated and characterized as undifferentiated mesenchymal cells with a phenotype fibroblasts-like and characteristic phenotype of confirmed by flow cytometry with CD44 +, CD105 +, CD271 +, CD34-and CD45-, plus the ability to differentiate into adipocytes, chondrocytes and osteocytes. Except for the control group (MSCs), in all groups, G-PRP (PRP and MSCs), G-DBM (DBM and MSCs) and G-PRP + DBM (MSCs and PRP + DBM) was differentiation osteoblastic with production of mineralized bone matrix confirmed by Alizarin red staining. The measurement of alkaline phosphatase (AP) increased up to 14 days in all groups. Bone proteins were expressed in the polymerase chain reaction in real time and were able to confirm the differentiation bone in all groups. Osteopontin had its greatest expression in G- PRP to seven days followed by G-PRP + DBM, at other times the G-PRP + DBM had higher expression than the other groups. The osteocalcin and Runx2 were expressed more intensely in the G-PRP + DBM at the end of the experiment. It can be concluded that the PRP and DBM are capable of promoting osteogenesis mesenchymal cells in Wharton's jelly of the umbilical cord with canine, with advantage in the presence of DBM
Orientador: Alexandre Lima de Andrade
Banca: Rita Cássia Menegati Dornelles
Banca: Márcio Mateus Beloti
Doutor
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Serra, Martínez Yolanda. "Diseño y creación de un programa de promoción para la salud para incrementar el cribado de cáncer colorrectal en Puerto Rico. Siguiendo los pasos de Intervention Mapping." Doctoral thesis, Universitat de Lleida, 2018. http://hdl.handle.net/10803/586263.
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Introducció
El càncer colorrectal (CCR) lidera les causes de mortalitat per càncer a Puerto Rico (PR). Malgrat ser un càncer que es pot prevenir amb facilitat mitjançant el seu cribratge i tractament de pòlips precancerosos, la incidència i mortalitat per aquest càncer segueixen creixent a l'Illa, alhora que les ràtios de cribratge romanen baixes. L'objectiu principal d'aquesta tesi doctoral és descriure el procés de disseny i creació de components educatius per a un programa de promoció per a la salut que pretén incrementar el cribratge del CCR en pacients de 50 anys o més pertanyents als Centres Federals de Salut Primària (Federally Qualified Health Centers; FQHC) de PR.
Els mètodes
Per això es va utilitzar Intervention Mapping (IM), un marc de planificació sistemàtica basat en la teoria i en l'evidència que ens guià en la planificació i disseny d'un programa de promoció per a la salut, la finalitat de la qual és incrementar el cribratge de CCR entre els Porto-riquenys de 50 anys o més, que siguin pacients dels FQHCs. IM consisteix en sis passos amb tasques específiques. En aquesta tesi es presenten els quatre primers (valoració de necessitats, matriu d'objectius de canvi, selecció de mètodes i estratègies, i creació del programa). Els passos 5 i 6 (implementació i avaluació del programa) es plantegen com a futures fases de la investigació.
Resultats
En un primer pas, es van determinar la incidència i les taxes de mortalitat de CCR mitjançant informació del Registre de Càncer de PR. Es va dur a terme una revisió de la literatura per comprendre millor els determinants personals i factors de l'entorn relacionats amb el CCR i el seu cribratge tant a Puerto Rico com a poblacions llatines dels Estats Units i, finalment, es van recollir noves dades mitjançant grups focals. Vàrem conduir 7 grups focals (N = 56) per identificar les necessitats i recursos de la població, sub- comportaments específics (objectius d'acció) i determinants relacionats amb el cribratge del CCR. En un segon pas, creàrem les matrius d'objectius de canvi que guiaran el contingut. En un tercer pas, seleccionàrem els mètodes generals d'entreteniment educatiu i periodisme conductual, i desenvolupàrem aplicacions pràctiques, materials i missatges que, al mateix temps, contenien altres mètodes com el modelatge, la persuasió, la informació i el tailoring, i es va determinar el tipus de format per a cada component educatiu. En el pas 4, desenvolupàrem una intervenció multimèdia interactiva adaptada (tailored interactive multimedia intervention; TIMI), una revista, diversos fulls informatius, infogràfics, i un pla d'acció. De la mateixa manera, vàrem crear un recordatori per a proveïdors de salut, mediat pels propis pacients, amb la finalitat d'augmentar la recomanació dels metges sobre el cribratge del CCR i, alhora, millorar la comunicació pacient-proveïdor de salut; i dissenyàrem un protocol de trucades de recordatori de les proves per a pacients. Finalment, es van dur a terme grups focals (N = 19) per valorar la usabilitat, atractiu, sensibilitat cultural i motivació generada pels components per realitzar les proves de cribratge. Les suggerències dels participants van ser valorades per l'equip d'investigació i incorporades a la versió final dels components educatius.Introducción El cáncer colorrectal (CCR) lidera las causas de mortalidad por cáncer en Puerto Rico (PR). A pesar de ser altamente prevenible mediante su cribado y el tratamiento de pólipos precancerosos, la incidencia y mortalidad por este cáncer sigue creciendo en la Isla, mientras que las ratios de cribado permanecen bajas. El objetivo principal de esta tesis doctoral es describir el proceso de diseño y creación de componentes educativos para un programa de promoción para la salud que pretende incrementar el cribado del CCR en pacientes de 50 años o más pertenecientes a los Centros Federales de Salud Primaria (Federally Qualified Health Centers; FQHC) de PR. Métodos Para ello se utilizó Intervention Mapping (IM), un marco de planificación sistemática basado en la teoría y en la evidencia que nos guio en la planificación y diseño de un programa de promoción para la salud cuya finalidad es incrementar el cribado de CCR entre los puertorriqueños de 50 años o más que sean pacientes de los FQHC. IM consta de seis pasos con tareas específicas. En esta tesis se presentan los cuatro primeros (valoración de necesidades, matriz de objetivos de cambio, selección de métodos y estrategias y creación del programa). Los pasos 5 y 6 (implementación y evaluación del programa) quedan planteados como futuras fases de la investigación. Resultados En un primer paso, determinamos la incidencia y las tasas de mortalidad de CCR usando información el Registro de Cáncer de PR. Condujimos una revisión de la literatura para entender mejor los determinantes personales y factores del entorno relacionados con el CCR y su cribado en puertorriqueños y poblaciones latinas de Estados Unidos y, finalmente, recolectamos nuevos datos mediante grupos focales. Condujimos 7 grupos focales (N=56) para identificar las necesidades y recursos de la población, sub comportamientos específicos (objetivos de acción) y determinantes relacionados con el cribado del CCR. En un segundo paso, creamos las matrices de objetivos de cambio que guiaron el contenido. En un tercer paso, seleccionamos los métodos generales entretenimiento educativo y periodismo conductual, y desarrollamos aplicaciones prácticas, materiales y mensajes que, a su vez, contenían otros métodos como modelaje, persuasión, información y tailoring, y se determinó el tipo de formato para cada componente educativo. En el paso 4, desarrollamos una intervención multimedia interactiva adaptada (tailored interactive multimedia intervention; TIMI), una revista, diversas hojas informativas, infográficos, y un plan de acción. Del mismo modo, creamos un recordatorio para proveedores de salud, mediado por los propios pacientes, con la finalidad de incrementar la recomendación de los médicos acerca del cribado del CCR y, a su vez, mejorar la comunicación paciente-proveedor; y diseñamos un protocolo de llamadas de recordatorio de las pruebas para pacientes. Finalmente, se llevaron a cabo grupos focales (N=19) para valorar la usabilidad, atractivo, sensibilidad cultural, y motivación generada por los componentes para realizarse las pruebas de cribado. Las sugerencias de los participantes fueron valoradas por el equipo de investigación e incorporadas a la versión final de los componentes educativos.
Introduction Colorectal cancer (CRC) is a leading cause of cancer- related mortality in Puerto Rico (PR). Despite being highly preventable through screening and treatment of precancerous polyps, the incidence and mortality from CRC in PR continues to grow while screening rates remain low. The objective of this dissertation is to describe the process of designing and creating educational components for a health promotion program that aims to increase CRC screening in patients aged 50 years or older belonging to the Federally Qualified Health Centers (FQHC) in PR. Methods We used Intervention Mapping (IM), a systematic framework using theory and evidence to plan and design a health promotion program that aims to increase CRC screening among Puerto Ricans 50 years or more who are patients of the FQHC. IM consists of six steps with specific tasks. This dissertation presents the first four (needs assessment, matrix of change objectives, selection of methods and strategies and creation of the Program). Steps 5 and 6 (program implementation and evaluation) are considered as future phases of the research. Results To inform the development of a logic model of the problem during the needs assessment phase, we determined the CRC incidence and mortality rates in Puerto Rico using recent data from the PR Cancer Registry, conducted a literature review to better understand behavioral and environmental factors influencing CRC among Hispanics in general and in Puerto Ricans, and collected new data. We conducted 7 focus groups (n=56) to identify community needs and resources, specific sub behaviors related to CRCS (performance objectives) and the determinants of CRCS. We then developed matrices of change objectives that would guide the content, behavioral change method selection and the practical applications that would be included in the program. We selected two overarching methods: entertainment education and behavioral journalism and developed practical applications, materials and messages containing several other methods including modeling, persuasion, information, and tailoring. We developed and pre-tested a tailored interactive multimedia intervention, newsletter, fact sheets, infographics, and an action plan. We also developed a patient mediated provider prompt to increase provider recommendation and improve patient provider communication and designed a protocol for support calls for patients. Finally, we conducted focus groups (N = 19) to assess the usability, attractiveness, cultural sensitivity, and motivation generated by the components to perform the screening tests. The suggestions of the participants have been evaluated by the research team and are being incorporated into the final version of the educational components.
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Souza, Talita Floering Brêda [UNESP]. "Diferenciação osteogênica de células mesenquimais do cordão umbilical canino." Universidade Estadual Paulista (UNESP), 2013. http://hdl.handle.net/11449/121939.
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000814146.pdf: 660875 bytes, checksum: 9336b38c8a05d65357c828a9a2773a9b (MD5)This study aimed to isolate MSCs from canine Wharton's jelly umbilical cord and evaluate in vitro the effect of PRP and the MOD in osteogenic differentiation of these cells in the absence of osteogenic medium. Cells were isolated and characterized as undifferentiated mesenchymal cells with a phenotype fibroblasts-like and characteristic phenotype of confirmed by flow cytometry with CD44 +, CD105 +, CD271 +, CD34-and CD45-, plus the ability to differentiate into adipocytes, chondrocytes and osteocytes. Except for the control group (MSCs), in all groups, G-PRP (PRP and MSCs), G-DBM (DBM and MSCs) and G-PRP + DBM (MSCs and PRP + DBM) was differentiation osteoblastic with production of mineralized bone matrix confirmed by Alizarin red staining. The measurement of alkaline phosphatase (AP) increased up to 14 days in all groups. Bone proteins were expressed in the polymerase chain reaction in real time and were able to confirm the differentiation bone in all groups. Osteopontin had its greatest expression in G- PRP to seven days followed by G-PRP + DBM, at other times the G-PRP + DBM had higher expression than the other groups. The osteocalcin and Runx2 were expressed more intensely in the G-PRP + DBM at the end of the experiment. It can be concluded that the PRP and DBM are capable of promoting osteogenesis mesenchymal cells in Wharton's jelly of the umbilical cord with canine, with advantage in the presence of DBM
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Hedlund, Simon. "“The Kingdom of God cannot be inherited by ἀρσενοκοῖται! (1 Cor 6:9)” : Who are they, and why is Paul condemning them?" Thesis, Uppsala universitet, Nya testamentets exegetik, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-254533.
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This paper will look at the interpretation and translation of ἀρσενοκοῖται (1 Cor 6:9) in the list of people who will not inherit the kingdom of God. The word is mentioned in 1 Cor 6:9-10, and it has been translated in ways going from “homosexual”, “men who lay with men” and “sodomite” to “a man who lets others use himself or who uses others for debauchery” and “pedophile”. By looking at the views on sexuality, and male same-sex sexuality in particular, pertaining the Greco-Roman society and the Jewish culture contemporary to Paul, and also paying attention to the textual context as well as the context of the congregation in Corinth, the study has its aim set on finding what the word might have denoted to the first readers in the congregation in Corinth, and to Paul. The goal, then, is not to find a translation of the word ἀρσενοκοῖται that is literal, but to find one that is as close to a dynamic equivalent as is possible. This goal will, hopefully, be attained by giving the translation to the modern reader that is the one most likely to connote the same ideas and emotional connotations as the Greek word did in its original context. As the meaning of words change with time and context, there is a need for a translation that can bridge the gap created by that shift of meaning. Building that bridge in the case of ἀρσενοκοῖται is the goal of this paper. When the meaning, or meanings, that is found most likely to have been attained by the ancient readers is found, there will be a critical evaluation of some of the modern translations (and some a bit older) of the word in 1 Cor 6:9 to see if there already exists a translation that can be said to reach the goal of dynamic equivalence, given the meaning that is found most plausible in this paper. The study will argue that that is not the case, and therefore also propose a new translation, one that is argued to be closer to dynamic equivalence than those that has been evaluated. This translation is ”Men who sexually exploit men to gain social powers”.Denna uppsats undersöker översättningen och tolkningen av ordet ἀρσενοκοῖται, det ord som avslutar 1 Kor 6:9 och är del av en lista över dem som inte kan ärva Guds rike (6:9-10). Ordet har översatts på olika sätt genom tiderna, med förslag som sträcker sig från “homosexuella”, “sodomiter”, “män som ligger med män” till “en man som låter utnyttja sig eller utnyttjar andra till osedlighet” och “pedofiler”. Genom att titta på hur sexualitet, och främst då manlig homosexualitet, sågs i den grekisk-romerska och judiska kontexten på Paulus tid, samt studera ordets litterära kontext och församlingens kontext i Korint, är målet att utröna vad ἀρσενοκοῖται bar med sig för betydelser och konnotationer för Paulus och de första mottagarna i Korint. Vad ett ord betyder är inte alltid statiskt över tid och rum utan förändras ofta, om än gradvis. Det riskerar därför att uppstå en klyfta mellan det som ordet från början innebar samt uppfattades som att det innebar, och hur det uppfattas idag. Den här uppsatsens mål är att överbrygga den klyftan för ἀρσενοκοῖται genom att ta reda på vad det grekiska ordet innebar i sin ursprungliga kontext och sedan ge en översättning som på bästa möjliga sätt ger en modern läsare konnotationer som i högsta möjliga grad stämmer överens med de konnotationer som de antika läsarna fick när de mötte ordet. Detta innebär alltså att målet inte är att ge en bokstavlig översättning av ἀρσενοκοῖται, utan en dynamisk ekvivalent översättning, där läsaren så långt det är möjligt får möjligheten att uppfatta det de första läsarna uppfattade. När den, eller de, meningar det är mest troligt att ordet bar med sig i sin ursprungliga kontext är konstaterade, kommer en kritisk utvärdering utifrån den slutsatsen göras av ett antal moderna översättningar (och några lite äldre), för att se om det finns en existerande översättning som uppnår dynamisk ekvivalens. Det kommer visa sig att så inte är fallet, och en ny översättning kommer därför att föreslås: ”Män som sexuellt utnyttjar män för att vinna social makt”.
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Domínguez, Solà David. "Mecanismes de regulació en l'activitat biològica del factor de transcripció Snail." Doctoral thesis, Universitat Pompeu Fabra, 2003. http://hdl.handle.net/10803/7065.
Full textAbstract:
Els factors de transcripció de la família Snail són fonamentals en la "transició epiteli-mesènquima", procés morfogènic essencial en el desenvolupament embrionari i en els fenòmens metastàsics tumorals.En els mamífers l'activitat d'Snail és modulada per dos mecanismes. (i) En el promotor humà es troben regions definides de resposta a factors repressors, predominants en les cèl·lules epitelials, i elements diferenciats de resposta a inductors de la "transició epiteli-mesènquima". (ii) L'activitat d'Snail és condicionada també per la seva localització subcel·lular, modulada per mecanismes no transcripcionals: la fosforilació d'Snail determina si és o no exclós del nucli. Al citosol no pot actuar com a repressor transcripcional però pot interaccionar amb la xarxa microtubular, que estabilitza i en condiciona el dinamisme. Això coincideix amb l'activació de la GTPasa RhoA i la reorientació dels filaments de vimentina, fets associats a l'adquisició de capacitat migratòria. L'efecte com a repressor transcripcional i la modulació del dinamisme microtubular són possiblement esdeveniments coordinats necessaris per al rol biològic d'Snail en mamífers.
Snail family of transcription factors is fundamental to the "epithelial-mesenchymal transition", morphogenic process essential to embryonic development and metastatic phenomena in tumors.
Snail's activity is modulated in two ways in mammals. (i) The human promoter harbors definite regions that respond to repressor factors, which prevail in epithelial cells; and differentiated elements that respond to known inducers of the "epithelial-mesenchymal transition". (ii) Snail's activity is also conditioned by its subcellular localization, mechanism not dependent on its transcriptional control: Snail phosphorylation determines whether Snail is excluded or not from the nucleus. When in the cytosol, Snail is unable to act as a transcriptional repressor, but however binds to the microtubular meshwork, which becomes stabilized and whose dynamism is conditioned as a result. This fact coincides with the activation of the RhoA GTPase and reorientation of vimentin filaments, both phenomena being related to the acquisition of cell motility. The transcriptional repressor and the microtubule dynamics effects are probably two coordinated events necessary to Snail's biological role in mammals.
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Lee, Yi-sin, and 李怡芯. "Inhibitory Effect of Red Mold Rice Extracts on Human Breast Cancer Cell Line (MCF-7)." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/42980137945171208028.
Full textAbstract:
碩士大仁科技大學
生物科技研究所
95
In this study, red mold rice extracts were analyzed for its effects on apoptosis in human breast cancer cell line, MCF-7. First, we used Monascus purpureus NTU 803 to ferment, and then extract by using ethanol and ethyl acetate. We used HPLC to analyze the content of monacolin K. The content of monacolin K in ethanol extract and ethyl acetate extract was 12.79 mg/g and 11.7 mg/g, respectively. In addition, we used the MTT method to determine the viability of red mold extracts on human breast cancer cells. Both extracts had inhibitory effect on the growth of cells. The results showed that the IC50 of ethanol extract and ethyl acetate extract for MCF-7 were 85 and 107 μg/mL. MCF-7 cells treated with red mold rice extracts revealed evident characteristics of apoptosis, which include cell blebbing, production of some tiny pellets, and formation of apoptotic bodies. DNA from treated MCF-7 cells displayed a characteristic inter-nucleosomal ladder. From the analysis of flow cytometry, cells treated with red mold rice extracts were arrested in the S phase of cell cycle. To conclude, red mold rice extracts can inhibit the growth of cells as well as the induction of apoptosis in human breast cancer line, MCF-7.
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Ho, Zu-Yen, and 何思妍. "Study of the fruiting body of rice-cultured Cordyceps militaris in inhibiting lung cancer growth." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/86314224682661189060.
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"A rice bran polyphenol, cycloartenyl ferulate, triggers caspase-dependent apoptosis in human colon cancer cells." Thesis, 2009. http://library.cuhk.edu.hk/record=b6074977.
Full textAbstract:
Findings from this pioneer study demonstrate that CF, which is unique to rice bran oil, is capable of triggering apoptosis in CRC cells at early stages of carcinogenesis. Furthermore, CF enhances TRAIL-induced apoptosis in metastatic CRC cells. This study provides clear evidence that the health-beneficial properties of whole grain consumption are not only limited by the presence of dietary fiber but also other molecules that can either act as a chemopreventive agent to directly induce tumor regression or a sensitizer to enhance TRAIL-induced apoptosis in metastatic cancer cells.High intake of whole grain food has been suggested as an important factor for reducing the risk of colon cancer, owing to the abundance of indigestible fiber. Rice bran, which is a component of raw rice after removal of starchy endosperm in milling process, has been shown to be a rich source of some health-beneficial compounds for preventing cancer, hyperlipidaemia, fatty liver, hypercalciuria, kidney stones, and heart disease (Jariwalla, 2001). In the present study, proliferation-inhibitory effects of some rice bran polyphenolic compounds were investigated on a panel of human colorectal cancer (CRC) cell lines, including SW480 (stage B), SW620 (stage C) and Colo-201 (stage D) with increasing metastatic potential according to the Dukes' classification system.
Results from the MTT study revealed that, among the polyphenolic compounds tested, cycloartenyl ferulate (CF) showed the most prominent proliferation inhibition on the CRC cells. CF is one of the typical ferulic acid esters of triterpene alcohols present in rice bran oil. The cancer cell proliferation was reduced by 62, 31 and 21% of their control levels after 72 h of 200 muM CF treatment, respectively. CF seemed to possess higher ability to control proliferation of tumor cells at early stages of cancer development. In meanwhile, results from Toxilight study showed that CF had low toxicity on normal colon CCD-18-Co cells. The anticancer activity of CF was further illustrated by its ability to induce significant regression of SW480 xenograft in nude mice. CF was found to induce apoptosis in SW480 cells in vitro. DNA flow cytometric studies demonstrated that CF elevated dose- and time-dependently the sub G1 or apoptotic cells with fragmented DNA. The pro-apoptotic effect of CF was further confirmed using immunoblotting study showing cleavage of poly(ADP-ribose) polymerase (PARP), which is a hallmark feature of apoptosis. Besides, the executioner procaspase-3, -6 and -7 were found to be processed and activated. On the other hand, administration of pan-caspase inhibitor Z-VAD-FMK completely rescued the cells from PARP cleavage, indicating that CF elicited solely caspase-dependent apoptosis. Elevation of death receptors DR4 and DR5 with the CF treatment seems to originate the upstream activation of the initiator procaspase-8 and -10 of the extrinsic apoptosis pathway. Flow cytometric JC-1 studies further demonstrated that CF significantly altered the mitochondrial membrane potential in a dose-dependent manner together with cytochrome c and smac/DIABLO but not AIF release from mitochondria into the cytosol, as well as the activation of procaspase-9 of the intrinsic apoptosis pathway. Depletion of anti-apoptotic Bcl-2 and elevation of pro-apoptotic Bak were observed; meanwhile, Bid was found to be cleaved by caspase-8, so that the death receptor pathway might be exaggerated by the mitochondrial apoptosis pathway.
Tumor necrosis factor-related apoptosis inducing ligand (TRAIL) is a promising candidate for cancer therapeutics due to its ability to induce apoptosis selectively in cancer cells (Gura, 1997). Result from MTT study illustrated that SW620 was more resistant than SW480 to TRAIL treatment. It is recognized that SW620 is the metastatic form of SW480 derived from the same patient at a later time, so it is important to develop agents that are able to sensitize the cancer cells to TRAIL, or to recover TRAIL sensitivity. We show for the first time that CF sensitizes SW620 cells to TRAIL-induced apoptosis and the mechanisms involved at least elevation of DR4, enhanced activation of caspase-8 and -3, as well as increase in DNA fragmentation.
Kong, Ka Lai.
Adviser: Wong Yum Shing.
Source: Dissertation Abstracts International, Volume: 73-01, Section: B, page: .
Thesis (Ph.D.)--Chinese University of Hong Kong, 2009.
Includes bibliographical references (leaves 119-136).
Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web.
Electronic reproduction. [Ann Arbor, MI] : ProQuest Information and Learning, [201-] System requirements: Adobe Acrobat Reader. Available via World Wide Web.
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Chao, Ying-Ting, and 趙盈婷. "Effects of Combinatory Products Fermented by Bacillus natto with Germinated Brown Rice against Human Colon Cancer Cells." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/18738034731586298982.
Full textAbstract:
碩士臺北醫學大學
醫學研究所
95
Rice is one of the most important grains, and more than one half of populations in the world consume rice as the staple food. Recently, people found that nutritional value is higher in unprocessed brown rice than in processed white rice. After germinating, the nutritional value of rice will be largely raised. Thus in this experiment, fermentation of Bacillus natto SYH-MT 0379 was preformed by using germinated brown rice as a main functional substrate for biotransformation. After a series of pretreatment steps were carried out to the fermentation broth, the fractionated portion of acidic ethyl acetate was detected to possess the active compounds with antimicrobial and antioxidant activities. Using TLC analysis followed by Diaion HP-20 and silica gel TLC separation, the active compound was purified at a mg level and thus named PGBRNFL-I. PGBRNFL-I exhibited a characteristic of fluorescence spot at UV 365 nm when analyzed by TLC plate. PGBRNFL-I possessed antimicrobial activities against all Gram positive and negative bacteria and also exhibited a significant scavenging effect on DPPH free radicals. Based on the fact that the antimicrobial activity of PGBRNFL-I was to abolish some compounds containing sulfhydryl (-SH), eg. reduced form of glutathione, which are ubiquitous existing in the cells and will accelerate the function of homeostasis balance to them. When disulfide bond or -SH group related to the intracellular macromolecules is attacked, DNA replication can be interfered which may cause cytotoxicity to some unlimited proliferate cells indirectly. Besides, colon cancer records a significant proportion of cancer deaths in Taiwan and is the third mortality. As a result, we chose human HT29 colon cancer cell line as our main target in representing the importance of colon cancer. The results of MTT together with the cytometric measurement of the intracellular ROS content to HT29 colon cancer cell line, indicated that PGBRNFL-I decreased cell growth in a dose-dependent manner via the involvement of ROS production which may induce cell death. As to fibroblast L929 cell, the MTT results indicated PGBRNFL-I had no cytotoxicity on it. The result of Western blot analysis showed that PGBRNFL-I suppressed the production of PMA-mediated COX-2 protein and also inhibited the expression level of nuclear factor-kappa B (NF-κB), all of which involved in the inflammatory disease. Altogether, this study presented a strongly support that the combinatory fermentation product of Bacillus natto and germinated brown rice is a promising source of functional food ingredient in application of cancer chemoprevention.
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Hsin-Ping and 林新萍. "Study of the inhibitory effects and mechanisms of black rice anthocyanins on liver cancer cell invasion and migration." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/89235803041415783982.
Full textAbstract:
碩士中山醫學大學
生化暨生物科技研究所
96
Metastasis is one of the major reason which causes death and after-effects of cancer. The invasion of metastasis is connected with proteolytic degradation of the extracellular matrix (ECM) and cell adhesion, essential to achieving cell motility. Anthocyanins, a large group of natrual polyphenols existing in a wide range of daily fruits and vegetables, have been widely recognized to possess several potential as cancer chemopreventive agents. Recent studies have also revealed pleiotropic anticancer and antiproliferative capabilities of anthocyanins. This study first demonstrates that effects of black rice anthocyanoins （Oryza sativa L. anthocyanins (OAs), peonidin 3-glucoside (P3G), and cyanidin 3-glucoside (C3G)）in SKHep-1 cells. We assayed the cell viability, cell invasion/motility, matrix metalloproteinases (MMPs) activity, and urokinase-plasminogen activator (u-PA) activity of SKHep-1 cells with flavonoid treatments by MTT assays, cell-matrix adhesion assays,invasion/motility assays, gelatin zymography, and casein zymography. We found that peonidin 3-glucoside and cyanidin 3-glucoside significantly inhibited the cell invasion/motility capacities and the activities of MMP-9 and u-PA in a concentration-dependent manner, while OAs would not affect cell viability . Following a treatment of cyanidin 3-glucoside coud significanty increase the tissue inhibitor matrix metalloproteinase-2 (TIMP-2) expression in SKHep-1 cells. To investigate the possible mechanisms involved in these events, we performed Western blot analysis and EMSA to find that peonidin 3-glucoside and cyanidin 3-glucoside suppressed the nuclear levels of c-Jun and c-Fos and the DNA binding activity of activator protein-1(AP-1) in SKHep-1 cells. Furthermore，peonidin 3-glucoside and cyanidin 3-glucoside also exerted an inhibitory effect of cell invasion and u-PA expression on various cancer cells(SCC-4、Huh-7、HeLa). Finally, an in vivo anti-tumor study using nude mice (BALB/c nu/nu) xenograft model by a subcutaneous inoculation of SKHep-1 cells was performed. The average tumor volume of treatment groups was statistically lower than that of control group. Together, these results suggest that cyanidin 3-glucoside, peonidin3-glucoside, or OAs may act to decrease the MMP-9 and u-PA expression of SKHep-1 cells via suppressing the nuclear levels of c-Jun and c-Fos and the DNA binding activity of AP-1 and therefore, reduce the invasion and metastasis of tumor cells. In conclusion, anthocyanins may be a powerful candidate for a preventive agent against liver cancer development and metastasis.
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Wei, Yi-Fang, and 魏怡芳. "The Effects of Preparation of Activated Canine Platelet-Rich Plasma by Low-Level Laser and Calcium Chloride and the Application of Activated Canine Platelet-Rich Plasma on Periodontal Diseases." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/8m58yh.
Full textAbstract:
碩士國立屏東科技大學
獸醫學系所
106
Platelet-rich plasma (PRP) is principally an increased concentration of autologous platelets suspended in anticoagulant blood after centrifugation. Platelets not only play an important role in hemostasis but are rich in growth factors. It was reported that the treatment effect is related to preparation and activation of platelets. The activation of platelets could be triggered by a variety of substances or stimuli such as low-level laser (LLL), thrombin, calcium chloride or collagen. However, the effects of LLL and calcium chloride on canine PRP are not reported. In addition, canine periodontal disease cause gingivitis, destruction of periodontal tissue, and loss of alveolar bone. Although we can cure gingivitis, but the periodontal tissue and alveolar bone injuries are irreversible. Therefore, the aim of the study was to evaluate the activated effects of LLL and calcium chloride on canine PRP, and the possibility to cure periodontal diseases in dogs. Blood samples were collected from 6 dogs. Each blood sample was divided into 8 groups: control group of plasma, and non-activated PRP, activated by calcium chloride, calcium chloride with 18 J laser, calcium chloride with 36 J laser, calcium chloride with 54 J laser, calcium chloride with 72 J laser, and calcium chloride with 90 J laser. Enzyme-Linked Immunosorbent Assay (ELISA) was used to detect the concentration of growth factor PDGF-BB, VEGF and TGF-β in activated PRP and plasma. According to the results, growth factors were significant upregulated through activation,however the LLL could not enhance the results which activated by calcium chloride alone. In clinical trials, PRP activated by calcium chloride was introduced to treat three dogs suffering from periodontal diseases. Before treatment, the severity of periodontal diseases were evaluated after dental scaling. Activated PRP was injected into alveolar mucosa of premolar and molar of right side and the degree of periodontal disease was re-evaluated on day 7, 14, 28 and 56 after treatment. Except bone mineral density and depth of bone loss, the calculus index, gingivitis index and probing depth had improved by using activated PRP. Therefore, using calcium chloride is adequate for preparation of activated canine PRP, and activated PRP can improve the results of periodontal treatment.
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Wang, Yi-Chin, and 王怡勤. "Study of the mechanisms of anthocyanidins from crude black rice extract in human oral tongue cancer CAL 27 cells." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/52855573522385154986.
Full textAbstract:
碩士亞洲大學
生物科技學系碩士班
100
There are many colored rice in rice germplasm. The pigments of colored rice are mainly located at the pericarp or seed coats of rice grain and they are anthocyanins pigments. The red cyanidins, orange pelargonidin, and purple-blue delphinidin are three major anthocyanins, which are reported to have anti-oxidative activity on lipid and scavenge activity for removing free radical. The results of HPLC analyses showed that the major anthocyanin is cyanidin-3-glucoside in the pericarp of black rice. Metastasis, the major cause of cancer death and various treatment strategies have targeted on preventing the occurrence of metastasis. The invasion of metastasis is connected with proteolytic degradation of the extracellular matrix(ECM) and cell adhesion, essential to achieving cell motility. Anthocyanins, a large group of natrual polyphenols existing in a wide range of daily fruits and vegetables, have been widely recognized to possess several potential as cancer chemopreventive agents. Recent studies have revealed pleiotropic anticancer and antiproliferative capabilities of anthocyanins. Therefore, it may be of great value in developing a potential cancer therapy for anthocyanins. Tumor metastasis is the most important cause of cancer death and various treatment strategies have targeted on preventing the occurrence of metastasis. The anti-metastatic mechanism of anthocyanins on the invasion/migration of human oral CAL 27 cells which were performed by used a Transwell to quantify the migratory potential of CAL 27 cells and we found that anthocyanins can inhibit in vitro migration and invasion of CAL 27 cancer cells. Besides, the gelatin zymography assay indicated that anthocyanins inhibited the activity of matrix metalloproteinases 2(MMP-2). Western blotting assay also showed that anthocyanins inhibited the associated protein expression of migration/invasion of CAL 27 cell. Immunofluorescence staining proved that anthocyanins inhibited Nuclear factor kappa B-p65(NF-κB p65)expressions. These results demonstrated that anthocyanins could suppress CAL 27 cell metastasis by reduction of MMP-2, MMP-9,u-PA and NF-κB p65 expression through the suppression of PI3K/Akt pathway and inhibition of NF-κB levels.
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Lantz, Paula M. "Breast cancer on the rise a socio-epidemiologic analysis of mammography screening and the increased incidence of breast cancer /." 1991. http://catalog.hathitrust.org/api/volumes/oclc/26560486.html.
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Thesis (Ph. D.)--University of Wisconsin--Madison, 1991.Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (158-164).
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Ferreira, Liliana Manuela Santos. "Functional role of the small leucine-rich repeat proteoglycan (SLRP) biglycan in gastric cancer." Dissertação, 2020. https://hdl.handle.net/10216/131971.
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Ferreira, Liliana Manuela Santos. "Functional role of the small leucine-rich repeat proteoglycan (SLRP) biglycan in gastric cancer." Master's thesis, 2020. https://hdl.handle.net/10216/131971.
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Yu, Meng-Hsun, and 游孟勳. "The flavonoid-rich extract from Nelumbo Nucifera leave inhibits obesity factor induced breast cancer metastasis." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/87090573337200632712.
Full textAbstract:
碩士中山醫學大學
生化暨生物科技研究所
101
Breast cancer is the most common cancer in women in the Taiwan. Then breast cancer deaths accounted for 28.4% of all the cancer deaths, and have been associated with obesity. Previous studies, has been confirmed Nelumbo nucifera leave extract (NLE) can effectively reduce the fatty liver, atherosclerosis, hyperlipidemia and anti-oxidative properties. Thus, we investigated the relationship between obesity and breast cancer, and evaluated the inhibitory effect of NLE on the adipocytes-induced breast cancer progression. Adipocyte secreted conditioned medium (CM) noticeably induced breast cancer migration. NLE diminished the CM-induced migration number of breast cancer cell by boyden chamber assay. A direct correlation was found between adipocyte and breast cancer cell migration. Similarly, we used of ECIS assay, wound healing assay and transwell assay identical results were found. To investigate the molecular mechanisms of breast cancer cell migration using two-dimensional gel electrophoresis. We found CM increased the proteins level of the Hsp90, StarD10, Peroxiredoxin 6. The results suggest that CM stimulates migration of breast cancer cells, perhaps through the interaction of these proteins. Under the CM treatment, phosphorylated JNK, PI3K/Akt, StarD10 and Peroxiredoxin 6 levels of MCF-7 cells treated with NLE can significantly inhibited the protein expression were measured by Western blot analysis. These data indicate adipocytes provide a major component of the microenvironment for rapid tumor growth and migration, and this increase was inhibited significantly by NLE treatment. In summary, the study indicated that the future for the treatment of metastatic breast cancer, NLE inhibited cancer metastasis has developed into a process of natural drugs.
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Fitzgerald, Mickey. "The Rise and Demise of J.H. Todd and Sons, British Columbia’s Enduring Independent Salmon Canners." Thesis, 2015. http://hdl.handle.net/1828/6672.
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This thesis examines J. H. Todd & Sons Ltd., a British Columbia family-owned and run fish packing company during the years 1881 through 1954. The research presented in this paper outlines the contribution of individual Todd family members to both the success and eventual demise of the company. Also examined is the history of the B. C. salmon canning industry, the evolution of J. H. Todd & Sons Ltd., the role of the company in the broader context of the B. C. fishing industry; and the factors that led to the demise of the company. This thesis relies on documentary primary sources as well as an extensive oral history collection obtained from Todd family members and employees.Graduate
0334
0509
0510
micfitz@shaw.ca
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Santamaría, Carolina. "The impact of pesticide exposure on breast cancer incidence evidence from Costa Rica, 1996-2000 /." 2006. http://catalog.hathitrust.org/api/volumes/oclc/66388047.html.
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Thesis (M.S.)--University of Wisconsin--Madison, 2006.Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 51-58).
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"Cancer-caquexia e suplementação nutricional : impacto da dieta rica em leucina no controle do metabolismo proteico muscular." Tese, Biblioteca Digital da Unicamp, 2005. http://libdigi.unicamp.br/document/?code=vtls000353426.
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Lin, Tsui-Hwa, and 林翠華. "Calreticulin Regulates Fucosyltransferase 1 Expression in Bladder Cancer Cell through A-U rich Element of 3’UTR." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/73317067289591772402.
Full textAbstract:
碩士國立臺灣大學
動物學研究所
100
Calreticulin (CRT) localizes to intracellular, cell surface and extracellular compartments and regulates a variety of diverse and important biological processes. CRT ensures proper folding of proteins and glycoproteins regulating homeostatic control of cytosolic and ER calcium levels. CRT is necessary for cell adhesion, migration and phagocytosis of apoptotic cells. CRT can be detected in urine of bladder cancer patients as a cancer marker. Higher concentration of urinary CRT tends to increase pathologic stages of tumor. Down-regulation of CRT suppresses tumor growth and metastatic capacities in human bladder cancer. Glycosylation of integrin modulates the binding strength of integrin and extracellular matrix (ECM) binding. Fucosyltransferase 1 (FUT1) expression and fucosylation of β1 integrin, catalyzed by FUT1, were down-regulated when CRT in human bladder cancer cells was knocked down (data unpublished). Suppression of FUT1 expression and fucosylation of β1 integrin may lead to modulation of tumor cell attachment and tumor malignances. Real-time PCR and mRNA degradation assays have indicated that half-life of FUT1 mRNA is much shorter in bladder cancer cells transfected with CRT-RNAi than in the control cells. These data implicated that the suppression of FUT1 expression might due to instability of FUT1 mRNA. In this IV study, we built EGFP-FUT1 3’UTR constructions with wild type adenylate-uridin rich element (ARE), mutated ARE and deleted ARE respectively. By transfecting each of these three constructions in J82 bladder cancer cells, we found that CRT plays a crucial role in modulating FUT1 mRNA stability through interacting with ARE in 3’UTR of FUT1 mRNA.
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Ya-Fung and 鄭雅方. "Nuciferine Leaf Polyphenol rich-extract induced human breast cancer cell apoptosis involving p38 MAPK / Fas L pathway." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/97395716625184769769.
Full textAbstract:
碩士中山醫學大學
生化暨生物科技研究所
94
According to the research from the Department of Health of Executive Yuan, cancer has been the first reason of death in Taiwan. Therefore, it is a popular topic of discussing how to prevent and postpone the formation of cancer. Nowadays, most researches tend to use natural materials to control the growing and aggravation of the cancer cell. Many studies demonstrate that Polyphenols in plants have the effect of antioxidation and anticarcinogenesis, such as the Polyphenols in the Hibiscus sabdanffa L. ,green tea ,red wine and apples. This study first extracted the polyphenols from the Nelumbo nucifera leaves to use as qualitative and quantitative。In the qualitative part, the calculated amount of polyphenols extraction was about 85-95%. As for the quantitative part, the polyphenols ingredient in Nelumbo nucifera leaves was analyzed with HPLC. And then the NPRE was sieved using nine different cancer cell line, including Hep G2, Hep 3B, KB, Caco-2, HL-60, AGS, NIH 3T3, MCF-7 and MDA-231. The results indicated that MCF-7 was the most sensitive to inhibit the survival ratio of the cell (IC50 = 0.63 mg/ml) in a dose-dpendent manner. Next, the MCF-7 cell was used as the experimental model to observe the apoptosis phenomena under the circumstances of different concentrations of NLE and NPRE. It is discovered that the more NLE and NPRE consistency increased, the more conspicuous apoptosis became, including the changes of the morphology and the DNA fragmentation. Analyzing with Flow cytometric, we found that under the same amount, the NLE caused apoptotic cell of MCF-7 and the cell cycle arrest in the G0/G1 phase while the NPRE caused the death of MCF-7. But the results could be blocked by the inhibitors SB203580 which made the declining of MCF-7 induced by NPRE slow from 44.44% down to 17%. The recovery rate is about 60%. Therefore, it is estimated that the p38 protein may play an important role in the declining pace of MCF-7 which induced by NPRE. The same result to occuring in the Western Blots analysis, we found that the p38、Jun and p53 protein were activation by NPRE , which means they also could be blocked by the p38 inhibitor and decline in p38、Jun and p53 protein.
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Hsu, Hung Chih, and 徐鴻智. "The role of Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5) in colorectal cancer pathogenesis." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/66903441348695248007.
Full textAbstract:
博士長庚大學
臨床醫學研究所
103
Colorectal cancer (CRC) is the most prevalent form of cancer among Taiwanese and one of leading cause of cancer deaths with a worldwide cumulative incidence rate of 9.4%. The leucine-rich repeat-containing G protein coupled receptor 5 (LGR5) is not only an adult intestinal stem cell but also a CRC stem cell marker. CRC stem cell often account for therapy resistance. However, the value of LGR5 level in CRC prognosis and pathogenesis has not been well characterized. In addition, lysine-specific demethylase1 (LSD1) was highly expressed in several malignancies and had been implicated in pathological processes of cancer cells. LSD1 inhibitor was reported to selectively inhibit the growth of several cancer stem cells. Thus in this study, we proposed to evaluate the role of LGR5 in pathogenesis and prognosis of CRC. We also used an LSD1 inhibitor, CBB1003, as a probe, to assess the association between LSD1 and LGR5 in CRC carcinogenesis. Our study found that elevated LGR5 level was associated with poor survival and chemoresistance in CRC patients. We also demonstrated that LGR5 was a functional CRC stem cell marker and regulated CRC cell survival via modulating Wnt/β-catenin signaling and intrinsic mitochondrial apoptotic pathway. Furthermore, we found that LSD1 overexpression promotes CRC development and that the LSD1 inhibitor inhibits CRC cell growth by downregulating LGR5 levels and inactivates the Wnt/β-catenin pathway. These results suggested that LGR5 could be a marker to predict clinical outcome and therapy response in CRC patients. Due to a functional CRC stem cell marker, LGR5 may also serve as a potential therapeutic target for CRC therapy. In addition, LSD1 and its inhibitor might provide a new target or a useful strategy for therapy of CRC.
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Hung-Ta, Tsang, and 臧宏達. "Development of the product of lactic acid fermented germinative brown rice milk and study its effect of inhibition of Caco-2 colorectal cancer cell proliferation." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/06467630662055477899.
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碩士實踐大學
食品營養研究所
92
Rice is the most important farm product in Asia and Taiwan. It is also the staff of life. According to the statistic data calculate by Council of Agriculture in Taiwan. Taiwan produces 1.53 million tons of brown rice per year. The aim of this study was adopt the Taiwan local rice to develop a kind of function food. In the brown rice germination and Somogi study. We choice Taikeng 2 (TK2) as the species which has the highest germination ability and fermentable sugar content. Then we abrasive the germination brown rice to get the 16% rice milk. It can have 3.8% fermented sugar and 225.6cp torque value. Next study we add Aspergillus oryzae fermented koji rice into the rice milk. The fermentable sugar is between 3.8~11.2%. Use 5~30% germination brown rice incubated with seven strains of mix lactic acid bacteria (Lactobacillus acidophilus, L. bulgaricus, Streptcoccus thermophilus etc.). Use the same subject to produce Monascus pilosus 31527 rice milk. This product which have the pH value between 3.56~3.7 and titratable acid between 3.65~4.57% . .Concentrate and extract the final product by methanol and acetone. Then get the brown rice (B), germination brown rice (GB), Aspergillus oryzae fermented germination brown rice (GBA), Monascus pilosus 31527 fermented germination brown rice (GBM) and lactic acid bacteria fermented Monascus pilosus 31527 fermented germination brown rice (GBML) concentrates. Bring it to treat the colorectal cell line Caco-2 and evaluate the ability to inhibit telomerase. The results showed that GBML has the strongest inhibition ability. As the GBML concentration up to 60μl/ml. The inhibition of the telomerase activity was up to 29.5%. In terms of SDS-page and western blotting, GBML have the inhibition ability to c-Myc and Bcl-2 in Caco-2. It also have the ability to increase Bax protein content in cell line. But the p21 and p27 is too weak to detect by quantification machine. In summary, the GBML can inhibit Caco-2 growth by decrease the c-Myc and Bcl-2 protein and increase Bax protein expression. But the treatment have no effect to p21 and p27 protein.
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陳美惠. "Down-regulation of proline-rich tyrosine kinase (Pyk2) expression decrease tumor malignancy in non-small cell lung cancer." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/70826806027311387086.
Full textAbstract:
碩士國立交通大學
分子醫學與生物工程研究所
100
The bioinformatics tool, the Gene Ontology analysis proposed proline-rich tyrosine kinase II (pyk2) as a candidate gene for tumor malignancy. pyk2, a second member of the focal adhesion tyrosine kinase-families, which is uniquely situated to act as a critical mediator for the activations of signaling pathways that regulate cell migration, proliferation, survival and angiogenesis. In this study, we down-regulated pyk2 expression of human lung cancer cells, A549, using small interfering RNA (siRNA)-expressing plasmids, and the transfectants were further investigated to examined its effects. Compared to non-treated or control siRNA-transfected cells, the results showed that inhibition of pyk2 expression suppressed survival, proliferation and migration ability of A549 cells.Therefore, Pyk2 might be associated with tumor malignancy and be a potential therapeutic target in cancer treatment.
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Chen, Ching-Chun, and 陳靜君. "Hibiscus sabdariffa Polyphenol-rich extract (HPE) via regulate CD44 signal pathway to anti-metastatic effect on human colon cancer." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/13398182322361961282.
Full textAbstract:
碩士中山醫學大學
生化暨生物科技研究所
101
In recent years, a natural plant extract an effective ingredient, thereby preventing or inhibiting the occurrence of cancer research has been the current trend. Roselle polyphenols Hibiscus sabdariffa Polyphenol-rich extract (HPE), one of the component in Roselle’s function, our previous studies indicate that HPE can effectively promote apoptosis on gastric cancer and colon cancer cell, the impact on tumor metastasis has not been clarified, so the next one probe into whether HPE inhibit cancer cell metastasis, and to explore the mechanism. We conducted MTT assay, using the DLD-1 colon cancer cells treated with different doses of HPE, observations DLD-1 cell viability, followed by DLD-1 cell as the experimental model, a fine cells induced lethal dose (IC50) of the HPE (1,2,3 mg / ml) for the following experiments. First observation of DLD-1 cells in wound healing experiments, we found that the higher the dose with the addition, DLD-1 cells crawling ability weaker; followed by cell invasion and metastasis experiments by boyden chamber and transwell assay results observed with the addition of the higher dose, DLD-1 cells, invasion and metastasis ability are increasingly inhibited. Zymography we used the experimental observations and related MMP and uPA expression that MMP-2 and uPA activity of HPE in a significant inhibitory effect, and a concentration-dependent manner. In order to clarify the molecular mechanism, we analysis cytoskeletal proteins, adhesion molecules, and the specificity of inhibition of Akt signal pathway to confirmed, HPE via regulating CD44 signaling pathway effectively inhibited DLD-1 colon cancer cell metastasis and invasion effect.
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Chen, Chih-Yu, and 陳致宇. "Calreticulin Regulates mRNA Stability via Interaction with AU-Rich Element at 3’-Untranslated Region in PC-3 Prostate Cancer Cells." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/2abk5e.
Full textAbstract:
碩士國立臺灣大學
生命科學系
107
Messenger RNA (mRNA) stability is a post-transcriptional regulation that determines mRNA fate and influences multiple cellular functions. Previous studies have demonstrated that AU-rich element (ARE) in 3’-untranslated region (3’-UTR) of mRNA is critical for mRNA stability via interaction with ARE-binding proteins (ARE-BPs). Chaperone protein calreticulin (CRT) has been reported to bind the 3’-UTR. The expression level of CRT is positively correlated with the expressions of angiogenic factor vascular endothelial growth factor-A (VEGF-A) and β1 integrin. However, the detailed regulatory mechanism of CRT on VEGF-A and β1 integrin expressions remains elusive. Therefore, this study aimed to investigate whether CRT regulates mRNA stability through interaction with ARE. Our results showed that depletion of CRT significantly destabilized VEGF-A and β1 integrin mRNA in PC-3 human prostate cancer cells, and subsequently decreased their expression in both mRNA and protein levels. Sequence analysis showed that ARE in found in the 3’UTR of both mRNA. The result of Luciferase reporter assay demonstrated that CRT interacts with ARE. RNA-immunoprecipitation analysis and electrophoretic mobility shift assay (EMSA) demonstrated that CRT indirectly interacts with mRNA. In summary, this study showed that CRT regulates mRNA stability through interacting with ARE at the 3’-UTR of mRNA.
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Wu, I.-Chiu, and 吳逸秋. "The Effects of Fractionated Ripe Areca Nut Extracts on the Expression of VEGF-D and IL-8 in Oral Cancer Cells." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/78200141308034483150.
Full textAbstract:
碩士國立陽明大學
藥理學研究所
91
Abstract IARC stated in 1985 that chewing areca quid with tobacco could lead to oral cancer. Many epidemiological studies indicated that chewing areca quid alone also correlated to the development of oral cancer in Taiwan. Polyphenols, the major components of areca nut, causes the astringent taste while chewing areca quid. Lime is added to areca quid to quench it but also can cause an alkaline environment to generate free radical. Recently it was found that fractionated ripe areca nut extracts (Fr. rANE) had more cytotoxic effects on OEC-M1 and OC-3 oral cancer cells under alkaline condition than cells cultured in normal medium. Using cDNA microarray technique, it was observed that the expression of several genes, including vascular endothelial growth factor-D (VEGF-D) and Interleukin-8 (IL-8) were down regulated after treatment of OEC-M1 cells with 50μg/ml Fr. rANE. This study then tested the effects of Fr. rANE on the expressions of VEGF-D and IL-8 in oral cancer cells by real-time PCR. The results demonstrated that Fr. rANE decreased the gene expressions of VEGF-D and IL-8 in OEC-M1 oral cancer cells in a dose-dependant manner. However in wild-type p53 OC-3 oral cancer cells, Fr. rANE increased the gene expressions of VEGF-D and IL-8. The VEGF-D mRNA expression was dramatically increased after treatment with 200μg/ml of Fr. rANE. The extracellular parameter pH values had no effect on the Fr. rANE modulation of VEGF-D and IL-8 gene expression in neither OEC-M1 nor OC-3 cancer cells. It is known that SC-M1 gastric cancer cells can secrete high levels of VEGF (1747.27 pg/ml) and express VEGF-R2 on its cell surface. Therefore, SC-M1 cells were used as a positive control to examine the effects of Fr. rANE and EGCG (a well-known angiogenesis inhibitor on the expression of VEGF-D and IL-8). Fr. rANE can modulate the VEGF and IL-8 gene expression as EGCG. Taken together, these results suggested Fr. rANE might have alternative effect for angiogenesis research.