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Relevant bibliographies by topics / Carbohydrate active enzymes (CAZymes) / Dissertations / Theses

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Dissertations / Theses on the topic 'Carbohydrate active enzymes (CAZymes)'

Author: Grafiati

Published: 4 June 2021

Last updated: 13 February 2022

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1

Venditto, Immacolata. "Structure and Function of novel Carbohydrate-Active Enzymes (CAZymes) and Carbohydrate-Binding Modules (CBMs) involved in Plant Cell Wall degradation." Doctoral thesis, Universidade de Lisboa. Faculdade de Medicina Veterinária, 2015. http://hdl.handle.net/10400.5/7894.

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Tese de doutoramento em Ciências Veterinárias. Especialidade de Ciências Biológicas e Biomédicas<br>ABSTRACT - Plant cell wall polysaccharides offer an abundant energy source efficiently utilized by a large repertoire of micro-organisms, which thus play a central role in carbon re-cycling. Aerobic micro-organisms secrete Carbohydrate-Active Enzymes (CAZymes) as free-standing proteins, whereas anaerobic bacteria organize a diverse enzyme consortium in a multi-component complex, the cellulosome, which performs a more efficient deconstruction of this composite structure. CAZymes are modular enzym

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2

Lopes, Vânia Alexandra da Silva Cardoso. "High-Throughput production and characterization of Carbohydrate-Active enZYmes for animal nutrition." Doctoral thesis, Universidade de Lisboa, Faculdade de Medicina Veterinária, 2020. http://hdl.handle.net/10400.5/19760.

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Tese de Doutoramento em Ciências Veterinárias na especialidade Produção Animal<br>The biodegradation of plant cell wall (PCW) carbohydrates is performed by microbial enzymes that are generally referred to as CAZymes. In animal nutrition, it is now well established that the monogastric animals produce a limited repertoire of CAZymes and as such cannot use efficiently some dietary ingredients that sometimes display antinutritional properties. The dietary supplementation with exogenous CAZymes improves the nutritive value of diets and increases animal’s performance. In particular, this study demo

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3

Larsbrink, Johan. "Strategies for the Discovery of Carbohydrate-Active Enzymes from Environmental Bacteria." Doctoral thesis, KTH, Glykovetenskap, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-126956.

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The focus of this thesis is a comparative study of approaches in discovery of carbohydrate-active enzymes (CAZymes). CAZymes synthesise, bind to, and degrade all the multitude of carbohydrates found in nature. As such, when aiming for sustainable methods to degrade plant biomass for the generation of biofuels, for which there is a strong drive in society, CAZymes are a natural source of environmentally friendly molecular tools. In nature, microorganisms are the principal degraders of carbohydrates. Not only do they degrade plant matter in forests and aquatic habitats, but also break down the m

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4

Fleites, Carlos Martinez. "Structural studies of carbohydrate active enzymes." Thesis, University of York, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.442417.

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5

Addington, Trevor. "Engineering carbohydrate-active enzymes: specificity and activity remodeled." Doctoral thesis, Universitat Ramon Llull, 2009. http://hdl.handle.net/10803/9285.

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To understand and modify the secondary cell walls of plants the project group Enzyme Discovery in Hybrid Aspen for Fiber Engineering (EDEN) was founded composed of nine laboratories with funding from the European Commission. The main target of EDEN´s research is to genetically engineer fiber structure in order to produce transgenic trees with modified properties for the pulp and paper industries. <br/>In this target framework, the Populus tremula x tremuloides xyloglucan endotransglycosylase (PttXET16A) was selected for in-depth study of its transglycosylase activity catalyzing cleavage and

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6

Hassan, Noor. "Characterization and engineering of carbohydrate-active enzymes for biotechnological applications." Doctoral thesis, KTH, Industriell bioteknologi, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-165613.

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Extremozymes are enzymes produced by microorganisms that live in extreme habitats. Due to their higher stability, extremozymes is attracting interest as biocatalysts in various industrial processes. In this context, carbohydrate-active extremozymes can be used in various processes relevant to the paper, food and feed industry. In this thesis, the crystal structure, biochemical characterization and the capacity to synthesize prebiotic galacto-oligosaccharides (GOS) were investigated for a β-glucosidase (HoBGLA) from the halothermophilic bacterium Halothermothrix orenii. The wild-type enzyme dis

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7

Hill, Anthony David. "Computational methods in the study of carbohydrates and carbohydrate-active enzymes." [Ames, Iowa : Iowa State University], 2006.

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8

Fernandes, Vânia Ondina Pedro. "Discovering novel carbohydrate-active enzymes in the cellulosome of anaerobic bacteria." Doctoral thesis, Universidade de Lisboa. Faculdade de Medicina Veterinária, 2015. http://hdl.handle.net/10400.5/9287.

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Tese de Doutoramento em Ciências Veterinárias, especialidade em Ciências Biológicas e Biomédicas<br>Carbohydrate-active enzymes (CAZymes) include a range of enzymes that, in nature, make, break or modify glycosidic bonds. CAZymes act on highly recalcitrant polysaccharides, such as cellulose and hemicellulose, and often exhibit a modular architecture including catalytic domains fused through flexible linker regions to non-catalytic domains such as carbohydrate-binding modules (CBMs). In some anaerobic bacteria these enzymes can associate in high molecular mass multi-enzyme complexes termed

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9

Gullfot, Fredrika. "On the engineering of proteins: methods and applications for carbohydrate-active enzymes." Doctoral thesis, KTH, Glykovetenskap, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-24296.

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This thesis presents the application of different protein engineering methods on enzymes and non-catalytic proteins that act upon xyloglucans. Xyloglucans are polysaccharides found as storage polymers in seeds and tubers, and as cross-linking glucans in the cell wall of plants. Their structure is complex with intricate branching patterns, which contribute to the physical properties of the polysaccharide including its binding to and interaction with other glucans such as cellulose. One important group of xyloglucan-active enzymes is encoded by the GH16 XTH gene family in plants, including xylog

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10

Kallas, Åsa. "Heterologous expression, characterization and applications of carbohydrate active enzymes and binding modules." Doctoral thesis, KTH, Träbioteknik, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3950.

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Wood and wood products are of great economical and environmental importance, both in Sweden and globally. Biotechnology can be used both for achieving raw material of improved quality and for industrial processes such as biobleaching. Despite the enormous amount of carbon that is fixed as wood, the knowledge about the enzymes involved in the biosynthesis, re-organization and degradation of plant cell walls is relatively limited. In order to exploit enzymes more efficiently or to develop new biotechnological processes, it is crucial to gain a better understanding of the function and mechanism o

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11

Kallas, Åsa. "Heterologous expression, characterization and applications of carbohydrate active enzymes and binding modules /." Stockholm, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3950.

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12

Raich, Armendáriz Lluís Adrià. "Unveiling Protein-Substrate Interactions and Conformations that Influence Catalysis in Carbohydrate-Active Enzymes." Doctoral thesis, Universitat de Barcelona, 2018. http://hdl.handle.net/10803/586173.

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Enzymes have attracted the attention of chemists and biologists since long ago due to their molecular complexity and tremendous efficiency. They are highly specific catalysts that make chemical reactions possible at mild conditions with an astonishing rate enhancement. For this reason there have been many efforts to understand how these macromolecules work, trying to find out crucial factors that influence their activity. Whereas it is difficult to settle on how enzymes work in general, with hot debates over many years, significant progress has been made in elucidating specific catalytic mecha

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13

Aspeborg, Henrik. "Discovery of fiber-active enzymes in Populus wood." Doctoral thesis, KTH, Biotechnology, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3801.

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<p>Renewable fibers produced by forest trees provide excellentraw material of high economic value for industrialapplications. Despite this, the genes and corresponding enzymesinvolved in wood fiber biosynthesis in trees are poorlycharacterized. This thesis describes a functional genomicsapproach for the identification of carbohydrate-active enzymesinvolved in secondary cell wall (wood) formation in hybridaspen.</p><p>First, a 3' target amplification method was developed toenable microarray-based gene expression analysis on minuteamounts of RNA. The amplification method was evaluated usingboth

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14

Rabausch, Ulrich [Verfasser], and Wolfgang [Akademischer Betreuer] Streit. "Metagenome derived carbohydrate active enzymes for directed modification of polyphenols / Ulrich Rabausch. Betreuer: Wolfgang Streit." Hamburg : Staats- und Universitätsbibliothek Hamburg, 2015. http://d-nb.info/1068931310/34.

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15

Baumann, Martin J. "Xyloglucan-active enzymes : properties, structures and applications." Doctoral thesis, Stockholm : Bioteknologi, KTH, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4314.

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16

Norell, Isabella. "Phaeodactylum tricornutum – Compositional Analysis, Carbohydrate-Active Enzymes and Potential Applications of Residual Algal Biomass from Omega 3 Production." Thesis, KTH, Industriell bioteknologi, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-292409.

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Microalgae are gaining more attention for several reasons such as being potential producers of sustainable fuel, for use as health supplements and in skincare. Simris Alg is a Swedish company that produces Omega 3 supplements from a primary producer of these fatty acids - the algal diatom Phaeodactylum tricornutum, which is a sustainable alternative to Omega 3 derived from fish. Omega 3 fatty acids constitute a small fraction of the total algal biomass, and to increase profitability and utilize all of the biomass, the purpose of this thesis project is to present potential applications for the

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17

Bäumgen, Marcus [Verfasser], Uwe T. [Akademischer Betreuer] Bornscheuer, Uwe T. [Gutachter] Bornscheuer, and Harry [Gutachter] Brumer. "Functional characterization of carbohydrate-active enzymes from marine bacteria / Marcus Bäumgen ; Gutachter: Uwe T. Bornscheuer, Harry Brumer ; Betreuer: Uwe T. Bornscheuer." Greifswald : Universität Greifswald, 2020. http://d-nb.info/1214368816/34.

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18

El, Kaoutari Abdessamad. "Exploration des enzymes du microbiome intestinal humain impliquées dans la dégradation des sucres complexes." Thesis, Aix-Marseille, 2013. http://www.theses.fr/2013AIXM5067/document.

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La présence de sucres complexes constitue une source nutritive importante pour le microbiote qui assure leur dégradation via des CAZymes. Dans le cadre de cette thèse, nous avons construit in silico un modèle de type minimicrobiome contenant 177 génomes représentatifs des communautés bactériennes dans un microbiote intestinal conventionnel. L’analyse du contenu de ce minimicrobiome nous a permis d’estimer leur abondance et leur diversité. De plus, la comparaison du contenu CAZymes par groupe bactérien de type « phylum » a révélé une variabilité inter-phylum, notamment une diversité de familles

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19

Reisky, Lukas [Verfasser], Uwe T. [Akademischer Betreuer] Bornscheuer, Uwe T. [Gutachter] Bornscheuer, Magali [Gutachter] Remaud-Simeon, and Emma [Gutachter] Master. "Discovery and Characterization of Novel Carbohydrate-Active Enzymes from Marine Bacteria / Lukas Reisky ; Gutachter: Uwe T. Bornscheuer, Magali Remaud-Simeon, Emma Master ; Betreuer: Uwe T. Bornscheuer." Greifswald : Universität Greifswald, 2019. http://d-nb.info/1188409018/34.

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20

Reisky, Lukas Verfasser], Uwe Theo [Akademischer Betreuer] [Bornscheuer, Uwe T. [Gutachter] Bornscheuer, Magali [Gutachter] Remaud-Simeon, and Emma [Gutachter] Master. "Discovery and Characterization of Novel Carbohydrate-Active Enzymes from Marine Bacteria / Lukas Reisky ; Gutachter: Uwe T. Bornscheuer, Magali Remaud-Simeon, Emma Master ; Betreuer: Uwe T. Bornscheuer." Greifswald : Universität Greifswald, 2019. http://nbn-resolving.de/urn:nbn:de:gbv:9-opus-27050.

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21

Kmit, Maria Carolina Pezzo. "Metagenoma do microbioma do rúmen de ovinos e prospecção de genes degradadores de biomassa vegetal." Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/11/11138/tde-01082018-110114/.

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O material lignocelulósico, presente na biomassa vegetal, representa uma importante fonte de energia, entretanto necessita da ação das enzimas lignocelulolíticas para sua degradação. A busca por novas enzimas que atuam na quebra da parede celular da planta em comunidades microbianas evoluídas naturalmente em um ambiente de degradação de biomassa como o rúmen oferece uma estratégia promissora para a prospecção de genes. Com isso, o projeto teve como objetivo a identificação de genes degradarores de biomassa vegetal em microrganismos do rúmen de ovinos usando a abordagem metagenômica. Para tanto

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22

Marynowska, Martyna. "Unravelling the termite digestion process complexity - a multi-omics approach applied to termites with different feeding regimes." Doctoral thesis, Universite Libre de Bruxelles, 2020. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/304812.

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With its unique consortium of microorganisms from all domains of life, termite gut is considered one of the most efficient lignocellulose degrading systems in nature. Recently, host diet and taxonomy as well as gut microenvironmental conditions have emerged as main factors shaping microbial communities in termite guts. The aim of this thesis was to investigate this highly efficient lignocellulolytic system at holobiont level, with a particular focus on gut microbiome function and composition in relation to the host diet. As a starting point, we optimised a complete framework for an accurate te

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23

(5930663), Casey A. Hooker. "Developing Anaerobic Fungi As a platform for Efficient lignocellulose hydrolysis." Thesis, 2019.

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<p>Lignocellulose is an ubiquitous source of fixed carbon that is presently underexploited for renewable energy technologies. Currently, producing enzyme cocktails that robustly degrade these feedstocks is a significant economic bottleneck. Anaerobic gut fungi native to the digestive tracts of ruminants and hindgut fermenters are widely understudied despite their inherent ability to degrade a significant portion (~50%) of the lignocellulose in herbivorous animals. Challenges in cultivation due to their strict oxygen sensitivity, and the lack of a central repository to maintain axenic stocks su

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24

Hekmat, Omid. "Mechanistic and proteomic studies of carbohydrate-active enzymes : β-1,4-glycanases and colletotrichum lindemuthianum chitin deacetylase". Thesis, 2005. http://hdl.handle.net/2429/17171.

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The field of proteomics is facing many challenges, one of which is the assignment of structure and function to all of the proteins encoded and expressed by prokaryotic and eukaryotic genomes. One approach to this involves the development of new methodologies for the activity-based protein profiling (ABPP) of enzymes. Glycoside hydrolases (GH) are one group of enzymes that still lack an efficient and specific mass spectrometry-based ABPP (MS-ABPP) method. A probe was synthesized which consists of a biotin tag, a disulfide-bonded spacer, and a 2-deoxy-2-fluoro-xylobioside inactivator that

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