Relevant bibliographies by topics / Carnobacterium divergens

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  2. Dissertations / Theses
  3. Book chapters

Academic literature on the topic 'Carnobacterium divergens'

Author: Grafiati
Published: 4 June 2021
Last updated: 14 February 2022

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Journal articles on the topic "Carnobacterium divergens"

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PILET, MARIE-FRANCE, XAVIER DOUSSET, RACHEL BARRÉ, GEORGES NOVEL, MICHEL DESMAZEAUD, and JEAN-CHRISTOPHE PIARD. "Evidence for Two Bacteriocins Produced by Carnobacterium piscicola and Carnobacterium divergens Isolated from Fish and Active Against Listeria monocytogenes." Journal of Food Protection 58, no. 3 (1995): 256–62. http://dx.doi.org/10.4315/0362-028x-58.3.256.

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Lactic acid bacteria (LAB) isolated from fish products (fresh fish, smoked and marinated fish, fish intestinal tract) were screened for bacteriocin production and immunity in conditions eliminating the effects of organic acids and hydrogen peroxide. Twenty-two isolates which were found to produce bacteriocin-like compounds were identified as Carnobacteria, Lactococci and Enterococci on the basis of morphological examination, gas production from glucose, growth temperatures, configuration of lactic acid, carbohydrates fermentation and deamination of arginine. Two Carnobacteria named V1 and V41 were selected for further studies and identified by DNA-DNA hybridization as Carnobacterium piscicola and Carnobacterium divergens, respectively. Their respective bacteriocins named piscicocin V1 and divercin V41 were heat-resistant and sensitive to various proteolytic enzymes. These bacteriocins were active against Listeria monocytogenes and exhibited a different spectrum of activity against LAB. Both bacteriocins had a bactericidal and non-bacteriolytic mode of action. Maximum production of piscicocin V1 and divercin V41 in Man Rogosa Sharpe (MRS) medium broth occurred at the beginning of the stationary phase and was higher at 20°C than at 30°C. When the cultures were maintained at pH 6.5, bacteriocin production was significantly increased.
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Worobo, R. W., M. J. Van Belkum, M. Sailer, K. L. Roy, J. C. Vederas, and M. E. Stiles. "A signal peptide secretion-dependent bacteriocin from Carnobacterium divergens." Journal of bacteriology 177, no. 11 (1995): 3143–49. http://dx.doi.org/10.1128/jb.177.11.3143-3149.1995.

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Rieder, Gabriele, Linda Krisch, Harald Fischer, Maria Kaufmann, Adolf Maringer, and Silja Wessler. "Carnobacterium divergens - a dominating bacterium of pork meat juice." FEMS Microbiology Letters 332, no. 2 (2012): 122–30. http://dx.doi.org/10.1111/j.1574-6968.2012.02584.x.

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YOUSSEF, M. K., C. O. GILL, F. TRAN, and X. YANG. "Unusual Compositions of Microflora of Vacuum-Packaged Beef Primal Cuts of Very Long Storage Life." Journal of Food Protection 77, no. 12 (2014): 2161–67. http://dx.doi.org/10.4315/0362-028x.jfp-14-190.

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Vacuum-packaged top butt cuts from a beef packing plant that does not use any carcass decontaminating interventions were assessed for their organoleptic and microbiological properties during storage at 2 or −1.5°C. Cuts stored at 2°C were acceptable after storage for 140 days but were unacceptable after 160 days because of persistent sour, acid odors. Odors of cuts stored at −1.5°C for 160 days were acceptable. The numbers of aerobes on cuts increased from <1 log CFU/cm2 to 7 or 6 log CFU/cm2 for cuts stored at 2 or −1.5°C, respectively. The numbers of Enterobacteriaceae increased from < −1 log CFU/cm2 to 5 or 3 log CFU/cm2 for cuts stored at 2 or −1.5°C, respectively. Bacteria recovered from initial microflora were, mainly, strictly aerobic organisms. Bacteria recovered from cuts stored for 160 days were mainly Carnobacterium spp. that grew on an acetate-containing agar generally selective for lactic acid bacteria other than Carnobacterium. C. divergens and C. maltaromaticum were recovered from cuts stored at 2°C, but C. maltaromaticum was the only species of Carnobacterium recovered from cuts stored at −1.5°C. No lactic acid bacteria of genera that usually predominate in the spoilage microflora of vacuum-packaged beef at late storage times were recovered from the spoilage microflora. The findings indicate that carnobacteria, initially present at very small numbers, grew exponentially to persistently dominate the spoilage microflora of vacuum-packaged beef cuts of unusually long storage life.
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DUFFES, FRÉDÉRIQUE, CHRISTIAN CORRE, FRANÇOISE LEROI, XAVIER DOUSSET, and PATRICK BOYAVAL. "Inhibition of Listeria monocytogenes by In Situ Produced and Semipurified Bacteriocins of Carnobacterium spp. on Vacuum-Packed, Refrigerated Cold-Smoked Salmon." Journal of Food Protection 62, no. 12 (1999): 1394–403. http://dx.doi.org/10.4315/0362-028x-62.12.1394.

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Listeria monocytogenes inhibition by Carnobacterium strains and crude bacteriocins on sterile and commercial vacuum-packed cold-smoked salmon stored at 4°C and 8°C was investigated. Carnobacterium piscicola V1 was bactericidal against L. monocytogenes at the two temperatures, whereas Carnobacterium divergens V41 presented a bacteriostatic effect. C. piscicola SF668 delayed L. monocytogenes growth at 8°C and had a bacteriostatic effect at 4°C. Listeria growth was not affected by a non–bacteriocin-producing C. piscicola. Crude extracts of piscicocins were bactericidal at 4°C and 8°C. Listeria growth was delayed by divercin V41 at 8°C and was inhibited at 4°C. Nisin delayed Listeria growth at 8°C and was bacteriostatic at 4°C. The present study demonstrates that L. monocytogenes growth could be prevented on vacuum-packed cold-smoked salmon by Carnobacterium and associated bacteriocins at chilled temperatures. Moreover, no product spoilage could be observed with the use of such bacteriocin-producing strains as demonstrated by good sensorial analyses and low biogenic amine production.
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Rachman, Cinta, Petia Kabadjova, Rosica Valcheva, Hervé Prévost, and Xavier Dousset. "Identification of Carnobacterium Species by Restriction Fragment Length Polymorphism of the 16S-23S rRNA Gene Intergenic Spacer Region and Species-Specific PCR." Applied and Environmental Microbiology 70, no. 8 (2004): 4468–77. http://dx.doi.org/10.1128/aem.70.8.4468-4477.2004.

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ABSTRACT The genus Carnobacterium is currently divided into the following eight species: Carnobacterium piscicola, C. divergens, C. gallinarum, C. mobile, C. funditum, C. alterfunditum, C. inhibens, and C. viridans. An identification tool for the rapid differentiation of these eight Carnobacterium species was developed, based on the 16S-23S ribosomal DNA (rDNA) intergenic spacer region (ISR). PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of this 16S-23S rDNA ISR was performed in order to obtain restriction profiles for all of the species. Three PCR amplicons, which were designated small ISR (S-ISR), medium ISR (M-ISR), and large ISR (L-ISR), were obtained for all Carnobacterium species. The L-ISR sequence revealed the presence of two tRNA genes, tRNAAla and tRNAIle, which were separated by a spacer region that varied from 24 to 38 bp long. This region was variable among the species, allowing the design of species-specific primers. These primers were tested and proved to be species specific. The identification method based on the 16S-23S rDNA ISR, using PCR-RFLP and specific primers, is very suitable for the rapid low-cost identification and discrimination of all of the Carnobacterium species from other phylogenetically related lactic acid bacteria.
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Vihavainen, Elina, Hanna-Saara Lundstr�m, Tuija Susiluoto, et al. "Role of Broiler Carcasses and Processing Plant Air in Contamination of Modified-Atmosphere-Packaged Broiler Products with Psychrotrophic Lactic Acid Bacteria." Applied and Environmental Microbiology 73, no. 4 (2006): 1136–45. http://dx.doi.org/10.1128/aem.01644-06.

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ABSTRACT Some psychrotrophic lactic acid bacteria (LAB) are specific meat spoilage organisms in modified-atmosphere-packaged (MAP), cold-stored meat products. To determine if incoming broilers or the production plant environment is a source of spoilage LAB, a total of 86, 122, and 447 LAB isolates from broiler carcasses, production plant air, and MAP broiler products, respectively, were characterized using a library of HindIII restriction fragment length polymorphism (RFLP) patterns of the 16 and 23S rRNA genes as operational taxonomic units in numerical analyses. Six hundred thirteen LAB isolates from the total of 655 clustered in 29 groups considered to be species specific. Sixty-four percent of product isolates clustered either with Carnobacterium divergens or with Carnobacterium maltaromaticum type strains. The third major product-associated cluster (17% of isolates) was formed by unknown LAB. Representative strains from these three clusters were analyzed for the phylogeny of their 16S rRNA genes. This analysis verified that the two largest RFLP clusters consisted of carnobacteria and showed that the unknown LAB group consisted of Lactococcus spp. No product-associated LAB were detected in broiler carcasses sampled at the beginning of slaughter, whereas carnobacteria and lactococci, along with some other specific meat spoilage LAB, were recovered from processing plant air at many sites. This study reveals that incoming broiler chickens are not major sources of psychrotrophic spoilage LAB, whereas the detection of these organisms from the air of the processing environment highlights the role of processing facilities as sources of LAB contamination.
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Begrem, Simon, Flora Ivaniuk, Frédérique Gigout-Chevalier, et al. "New Insight into Antimicrobial Compounds from Food and Marine-Sourced Carnobacterium Species through Phenotype and Genome Analyses." Microorganisms 8, no. 7 (2020): 1093. http://dx.doi.org/10.3390/microorganisms8071093.

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Carnobacterium maltaromaticum and Carnobacterium divergens, isolated from food products, are lactic acid bacteria known to produce active and efficient bacteriocins. Other species, particularly those originating from marine sources, are less studied. The aim of the study is to select promising strains with antimicrobial potential by combining genomic and phenotypic approaches on large datasets comprising 12 Carnobacterium species. The biosynthetic gene cluster (BGCs) diversity of 39 publicly available Carnobacterium spp. genomes revealed 67 BGCs, distributed according to the species and ecological niches. From zero to six BGCs were predicted per strain and classified into four classes: terpene, NRPS (non-ribosomal peptide synthetase), NRPS-PKS (hybrid non-ribosomal peptide synthetase-polyketide synthase), RiPP (ribosomally synthesized and post-translationally modified peptide). In parallel, the antimicrobial activity of 260 strains from seafood products was evaluated. Among the 60% of active strains, three genomes were sequenced and submitted to a dereplication process. C. inhibens MIP2551 produced a high amountof H2O2, probably thanks to the presence of four oxidase-encoding genes. C. maltaromaticum EBP3019 and SF668 strains were highly efficient against Listeria monocytogenes. A new extracellular 16 kDa unmodified bacteriocin in the EBP3019 strain and five different bacteriocins in SF668 were highlighted. In this study, the overview of antimicrobial BGC and inhibitory activities of Carnobacterium spp. allowed the prediction of potential innovative natural products that could be relevant for biotechnological applications.
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Kabadjova, Petia, Xavier Dousset, Virginie Le Cam, and Hervé Prevost. "Differentiation of Closely Related Carnobacterium Food Isolates Based on 16S-23S Ribosomal DNA Intergenic Spacer Region Polymorphism." Applied and Environmental Microbiology 68, no. 11 (2002): 5358–66. http://dx.doi.org/10.1128/aem.68.11.5358-5366.2002.

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ABSTRACT A novel strategy for identification of Carnobacterium food isolates based on restriction fragment length polymorphism (RFLP) of PCR-amplified 16S-23S ribosomal intergenic spacer regions (ISRs) was developed. PCR amplification from all Carnobacterium strains studied always yielded three ISR amplicons, which were designated the small ISR (S-ISR), the medium ISR (M-ISR), and the large ISR (L-ISR). The lengths of these ISRs varied from one species to another. Carnobacterium divergens NCDO 2763T and C. mobile DSM 4849T generated one major S-ISR band (ca. 400 bp) and minor M-ISR and L-ISR bands (ca. 500 and ca. 600 bp, respectively). The ISRs amplified from C. gallinarum NCFB 2766T and C. piscicola NCDO 2762T were larger (S-ISR, ca. 600 bp; M-ISR, ca. 700 bp; and L-ISR, ca. 800 bp). The L-ISR contained two tDNAs coding for tRNAIle and tRNAAla genes. The M-ISR included one tRNAAla gene, and the S-ISR did not contain a tDNA gene. The RFLP scheme devised involves estimation of variable PCR product sizes together with HinfI, TaqI, and HindIII restriction analysis. Forty-two isolates yielded four unique band patterns that correctly resolved these isolates into four Carnobacterium species. This method is very suitable for rapid, low-cost identification of a wide variety of Carnobacterium species without sequencing.
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Groth Laursen, Birgit, Lene Bay, Ilse Cleenwerck, et al. "Carnobacterium divergens and Carnobacterium maltaromaticum as spoilers or protective cultures in meat and seafood: phenotypic and genotypic characterization." Systematic and Applied Microbiology 28, no. 2 (2005): 151–64. http://dx.doi.org/10.1016/j.syapm.2004.12.001.

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Dissertations / Theses on the topic "Carnobacterium divergens"

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Pilet, Marie-France. "Caracterisation de la divercine v41 et de la piscicocine v1, bacteriocines produites par carnobacterium divergens v41 et carnobacterium piscicola v1 isolees de produits marins." Caen, 1994. http://www.theses.fr/1994CAEN2045.

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Des bacteries lactiques isolees de produits marins (poissons frais conditionnes sous atmosphere modifiee, poissons fumes et marines, contenu intestinal de poissons) ont ete criblees pour la production de bacteriocines. Parmi 336 isolats vingt-deux se sont reveles producteurs de bacteriocines et ont ete identifies notamment a partir de leur morphologie, la presence d'acide meso-diaminopimelique dans leur paroi et leurs temperatures de croissance. Les isolats ont pu etre classes en trois groupes appartenant aux genres carnobacterium, lactococcus et enterococcus selon la classification usuelle. Les souches de carnobacterium ont ete identifiees comme carnobacterium divergens et carnobacterium piscicola par hybridation adn-adn. Les quatre bacteriocines presentent des spectres d'inhibition differents. Celles produites par les souches carnobacterium piscicola et carnobacterium divergens, appelees respectivement piscicocine v1 et divercine v41 sont actives vis a vis de bacteries comme listeria monocytogenes et clostridium tyrobutyricum. La divercine v41 et la 0piscicocine v1 ont ete purifiees a partir de surnageant de culture obtenu en milieu mrs sans tween, a 20c et a ph regule 6,5. La purification a ete realisee par precipitation au sulfate d'ammonium et chromatographie en phase inverse c18. La divercine v41 est un peptide de 4509 da comprenant environ 44 residus d'acides amines dont 38 ont ete determines. La purification de la piscicocine v1 a montre la presence d'au moins deux peptides appeles piscicocine v1a et v1b qui ont ete sequences partiellement. Les trois bacteriocines presentent des similitudes de sequences avec le groupe des bacteriocines anti-listeria. Le gene de structure de la divercine v41 a ete clone et sequence en partie. Cette bacteriocine est synthetisee sous forme d'un precurseur comprenant une sequence d'extension n-terminale de 23 residus qui se separe de la bacteriocine mature au niveau d'un site gly-gly, caracteristique des bacteriocines de bacteries lactiques non-lantibiotiques
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METIVIER, ANITA. "Etude de la divercine v41, bacteriocine produite par carnobacterium divergens v41 purification, caracterisation physico-chimique et genetique." Nantes, 1997. http://www.theses.fr/1997NANT2087.

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La divercine v41 (dv41) est une bacteriocine produite par cb. Divergens v41 qui inhibe les bacteries gram (+) et en particulier l. Monocytogenes. Dv41 est un peptide basique de masse moleculaire 4509 da. De type pediocine avec deux ponts disulfures : cys10-cys15 et cys25-cys43. Une nouvelle technique de purification de dv41, utilisant le partage de phase dans le triton x-114, a ete mise au point. Cette technique a donne d'excellents rendements permettant de realiser des etudes structurales et d'interactions avec les phospholipides par dichroisme circulaire et par spectroscopie de fluorescence. Dv41 est principalement dans un etat desordonne en solution aqueuse alors qu'en presence de lysopalmitoylphosphatidylcholine, la bacteriocine adopte une structure secondaire majoritairement sous forme de feuillets. La fluorescence des residus tryptophane a permis de mettre en evidence que dv41 interagit avec les micelles et les bicouches phospholipidiques. La polarisation de fluorescence du diphenylhexatriene a montre que dv41 pouvait penetrer dans la partie hydrophobe d'une bicouche lipidique. Les resultats experimentaux combines a des etudes de prediction de structure ont permis d'elaborer un modele structural de la dv41 dans une bicouche lipidique. Dv41 s'insere spontanement dans des bicouches planes de phospholipides et forme des canaux ioniques voltage-independants. Les determinants genetiques responsables de la production de la dv41 sont probablement chromosomiques. Le sequencage d'un fragment de 6 kb a permis de constater que le locus de la dv41 presente des particularites genetiques. Le gene de structure et le gene d'immunite sont separes par deux genes fusionnes. Le systeme de regulation de la dv41 est du type lantibiotique et non du type pediocine. L'orf codant le facteur accessoire, necessaire a la secretion de la bacteriocine, n'a pas ete identifie sur le fragment de 6 kb. L'application de la dv41 dans la conservation alimentaire semble delicate car elle selectionne rapidement des souches resistantes de listeria. Une etude prelimanaire visant a relier la resistance a la structure de la membrane a ete realise en etudiant la composition des lipides membranaires de souches sensibles et resistantes de l. Monocytogenes et de l. Innocua a la dv41.
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Dallaire, Laurent. "Optimisation des conditions de fermentation et de stabilisation pour la production de bio-ingrédients fonctionnels à base de Carnobacterium divergens M35." Master's thesis, Université Laval, 2017. http://hdl.handle.net/20.500.11794/37133.

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Lors de travaux antérieurs, un bio-ingrédient permettant la bioconservation du saumon fumé à froid et ayant une forte activité anti-Listeria fut développé et caractérisé. Il consiste en un milieu de culture fermenté par C. divergens M35 et contenant la bactériocine produite par la souche, soit la divergicine M35. Par contre, les conditions de production actuelles ne permettent pas une utilisation efficace et rentable de ce bio-ingrédient. L'objectif de ce travail est de répondre à ces problématiques. Dans un premier temps, un milieu de culture de grade alimentaire favorisant une forte et rapide croissance de C. divergens M35 et stimulant la production de la divergicine a été développé. Un criblage de différentes sources d'azote, de carbone et de sels a permis de déterminer que la mélasse de canne et le sucre de table (saccharose) sont les sources de carbone de choix, la source d'azote préférentielle reste l'extrait de levure et que l'acétate de sodium stimule la production en bactériocines. Ce milieu fut testé en fermenteur de 30L afin d'évaluer l'effet de la mise à l'échelle. Le milieu créé permet d'atteindre une biomasse de 9,04 log(UFC/mL) et une activité de 1,3X10⁵ AU/mL en 7h. Il s'agit d'une amélioration significative quant à la performance, mais aussi au coût du milieu de culture (0,89$/L) en comparaison à la référence, le milieu MRS (7$/L). Dans un deuxième temps, il a été démontré que le séchage par atomisation est bien plus efficace que la lyophilisation afin de produire un bio-ingrédient biologiquement stable. Le séchage par atomisation permet d'obtenir un bio-ingrédient sec possédant une viabilité de 9,85 log(UFC/g) et une activité anti-Listeria de 1,6X10⁶ AU/g. Ce procédé permet d'avoir une production rentable du bio-ingrédient M35.
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Naghmouchi, Karim. "Divergicine M35, une nouvelle bactériocine produite par Carnobacterium divergens M35 : caractérisation moléculaire du mécanisme d'action anti-microbien et du phénomène de résistance." Thesis, Université Laval, 2007. http://www.theses.ulaval.ca/2007/24296/24296.pdf.

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Richard, Christelle. "La divercine V41, une bactériocine de classe IIa produite par Carnobacterium divergens V41 : développement d'outils moléculaires et expression hétérologue chez E. coli." Nantes, 2004. http://www.theses.fr/2004NANT2007.

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Carnobacterium divergens V41, qui inhibe Listeria monocytogenes dans le saumon fumé, produit la divercine V41. Dans l'objectif d'étudier cette bactériocine de classe IIa, différents outils ont été mis en place. La production d'anticorps polyclonaux contre la divercine V41 a permis sa détection spécifique et sensible ainsi que sa purification par immunoaffinité. L'isolement d'une souche mutante de C. Divergens V41 (C. Divergens V41C9) a permis de démontrer que l'inhibition de L. Monocytogenes dans le saumon fumé par C. Divergens V41 était due à la divercine V41. L'analyse statistique du spectre d'action des bactériocines de classe IIa a mis en évidence la corrélation entre leur nombre de ponts disulfures et leur spectre d'activité. Un système d'expression hétérologue de la divercine V41 dans E. Coli à partir d'un gène synthétique a été développé avec succès. La divercine V41 recombinante dont les deux ponts disulfures sont reformés est pure, soluble et active contre Listeria
Carnobacterium divergens V41 which inhibits Listeria monocytogenes in smoked salmon, produces divercine V41. To study this class IIa bacteriocin, various tools were set up. Polyclonal antibodies generated against divercin V41 allowed its specific and significant detection and its purification by immunoaffinity. A mutant strain of C. Divergens V41 (C. Divergens V41C9) showed that L. Monocytogenes inhibition in salmon smoked by C. Divergens V41 was due to divercin V41. Statistical analysis of inhibition spectrum of class IIa bacteriocins highlighted important correlation between their number of disulfide bridges and their spectrum. Heterologous expression of divercin V41 in E. Coli with a synthetic gene was successfully developed. The recombinant divercin V41 whose two disulfide bridges, were reformed, was pure, soluble and active against Listeria
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Connil, Nathalie. "Étude physiologique et caractérisation génétique préliminaire de la production de tyramine par Carnobacterium divergens V41, souche au potentiel bioprotecteur dans le saumon fumé." Nantes, 2001. http://www.theses.fr/2001NANT2104.

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BHUGALOO, VIAL PARWIN. "Production et mode d'action de la divercine v41, une bacteriocine anti-listeria, secretee par carnobacterium divergens v41. Recherches preliminaires sur les mecanismes de resistance des cellules cibles." Rennes, Agrocampus Ouest, 1999. http://www.theses.fr/1999NSARI011.

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La divercine v41 (dv41) est une bacteriocine anti-listeria, produite par carnobacterium divergens v41. C'est un peptide cationique de 4509 da, de type pediocine, avec deux ponts disulfures. La production de la dv41 en continu a ete etudiee par quatre systemes differents. Parmi ces derniers, la production avec des cellules immobilisees s'avere nettement meilleure. Les etudes realisees sur les relations structure-activite, par modifications chimiques et enzymatiques, ont montre que les forces electrostatiques ne sont pas essentielles pour l'activite de la dv41. Le domaine cooh-terminal est actif seul et son activite inhibitrice repose sur son hydrophobicite et le repliement impose par le pont disulfure de ce domaine. Les acides amines aromatiques ont un role determinant, les residus tryptophanes etant plus importants que les residus tyrosines. Le motif consensus ygngv ne semble pas implique dans la reconnaissance d'un potentiel recepteur. Les spectroscopies de fluorescence et de dichroisme circulaire ont montre que l'activite de la dv41 est determinee par le packing des lipides et que son interaction depend plus de la composition lipidique globale de la membrane que d'un lipide specifique. L'analyse des lipides membranaires de la souche sauvage et d'un variant resistant a montre que la non susceptibilite de ce dernier peut etre du aux taux plus eleves en phosphatidylethnolamine, cardiolipine et moins eleve en phosphatidylglycerol. Un facteur de resistance, comprenant l'adn a ete mis en evidence. Cependant, la nature exact de ce dernier est a determiner car l'adn purifie de la souche resistante n'est pas capable de conferer seul la resistance chez la souche sensible.
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"Divergicine M35, une nouvelle bactériocine produite par Carnobacterium divergens M35 : caractérisation moléculaire du mécanisme d'action anti-microbien et du phénomène de résistance." Thesis, Université Laval, 2007. http://www.theses.ulaval.ca/2007/24296/24296.pdf.

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Book chapters on the topic "Carnobacterium divergens"

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Sip, A., and W. Grajek. "Production of Carnobacterium divergens biomass." In Progress in Biotechnology. Elsevier, 2000. http://dx.doi.org/10.1016/s0921-0423(00)80090-8.

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