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1
Anggi Putri Arina, I. Gusti Agung, Putu Timur Ina, and I. Gusti Ayu Ekawati. "Pengaruh Perbandingan Modified Cassava Flour (Mocaf) dan Puree Labu Kuning (Cucurbita Moschata) Terhadap Karakteristik Brownies Kukus." Jurnal Ilmu dan Teknologi Pangan (ITEPA) 10, no. 2 (June 30, 2021): 246. http://dx.doi.org/10.24843/itepa.2021.v10.i02.p08.
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The study aims to determine the effect comparison of mocaf with pumpkin puree to the characteristics of steamed brownies and to find out the best comparison mocaf with pumpkin puree to produce brownies with the best characteristics. The experimental design used in this research was a Completely Randomized Design (CRD) with a comparison of mocaf and puree of pumpkin consisting of 5 treatments namely: 100%: 0%; 90%: 10%; 80%: 20%; 70%: 30%; 60%: 40%. The treatment was repeated 3 times so that they were obtained 15 experimental units. The data obtained were analyzed by variance and if the treatment had significant effect then followed by Duncan’s Multiple Range Test (DMRT). Comparison of mocaf and pumpkin puree affected water content, ?-carotene, ?-carotene bleaching, hedonic test (color, texture, aroma, taste and overall rate) and scoring test (texture). Ratio of 60% mocaf and 40% puree of pumpkin had the best characteristics with water content of 34,49%, ash content of 1,10%, ?-carotene of 6,82 mg/100g, ?-carotene bleaching of 1,34 mg/ml, the texture was liked and very soft, the color, aroma, taste and the fullness of acceptance was liked.
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Tundis, Rosa, Khaled Rashed, Ataa Said, Francesco Menichini, and Monica R. Loizzo. "In vitro Cancer Cell Growth Inhibition and Antioxidant Activity of Bombax ceiba (Bombacaceae) Flower Extracts." Natural Product Communications 9, no. 5 (May 2014): 1934578X1400900. http://dx.doi.org/10.1177/1934578x1400900527.
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The flowers of Bombax ceiba were investigated for their chemical composition, antioxidant effects and antiproliferative activity against seven human cancer cell lines. The antiproliferative responses of diethyl ether (DE) and light petroleum (PE) extracts were evaluated by sulforhodamine B (SRB) assay against MCF-7, HeLa, COR-L23, C32, A375, ACHN, and LNCaP cells in comparison with a human normal cell line, 142BR. Moreover, extracts were characterized by GC-MS analysis and tested for their antioxidant properties by different in vitro systems, namely DPPH, Fe-chelating activity and β-carotene bleaching test. Both PE and DE extracts showed the highest antiproliferative activity against human renal adenocarcinoma (ACHN) in a concentration-dependent manner. PE extract showed the highest radical scavenging activity against the DPPH radical, while DE extract was more active in the β-carotene bleaching test. The presence of β-sitosterol and some fatty acids may contribute to the bioactivity of B. ceiba flower extracts.
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I, Lahmass, Ouahhoud S, Elyoubi M, Benabbas R, Sabouni A, Asehraou A, and Saalaoui E. "Evaluation of antioxidant activities of saffron stigma and spath as by-product of Crocus sativus L." MOJ Biology and Medicine 3, no. 4 (2018): 154–58. http://dx.doi.org/10.15406/mojbm.2018.03.00091.
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The present study aimed to estimate and compare, using in vitro assays, the antioxidant activities of alcoholic extracts of saffron stigma and spath as by-products of the saffron crop. The antioxidant activity was evaluated using in vitro assays such as total antioxidant capacity, DPPH free radical, Scavenging of H2O2, FRAP and β-carotene bleaching assay. The results allowed us to evidence the presence of products with antioxidant and bioactive capacities in spaths and stigmas of Crocus sativus. The response of each extract depends on the type of the test. Extract from spaths protect β-carotene from bleaching more than the extract of stigmas and extract from stigmas revealed a significant reducing power activity than that of spaths. As a result, we can consider these extracts especially that of spaths like a new source of antioxidant compounds.
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Benites, RSR, ASN Formagio, EJS Argandoña, CRF Volobuff, LNF Trevizan, MC Vieira, and MS Silva. "Contents of constituents and antioxidant activity of seed and pulp extracts of Annona coriacea and Annona sylvatica." Brazilian Journal of Biology 75, no. 3 (September 25, 2015): 685–91. http://dx.doi.org/10.1590/1519-6984.21313.
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AbstractThe antioxidant potential of fruit pulp and seeds of extracts of the Annona coriacea, and A. sylvatica(Annonaceae) were investigated, as well contents total phenolics, flavonoids, condensed tannins and ascorbic acid. Was used to determine the antioxidant activity the 1,1-diphenyl-1-picrylhydrazyl free radical (DPPH), β-carotene bleaching and ABTS radical cation method. The total phenol, total flavonoid, condensed tannin, and ascorbic acid contents were measured spectrophotometrically. In this study, the pulp and seeds of the fruits were extracted using methanol/water (8:2) for maceration. The seed extracts of A. coriacea demonstrated a moderate antioxidant effect with free radical scavenging activity of 31.53%, by the DPPH test, 51.59% by the β-carotene bleaching test and 159.50 µM trolx/g of extract in the ABTS assay. We found that the hydromethanolic seed extract of A. coriacea had high total phenol (147.08 ± 4.20 mg of GAE/g of extract) and flavonoid (131.18 ± 2.31 mg of QE/g of extract) content. This indicated that the antioxidant activity of the extracts was related to the contents of these constituents.
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Bouguerra, Ali, Samah Djebili, Nassim Zouaoui, and Malika Barkat. "Evaluation of phenolic contents and antioxidant activities of some medicinal plants growing in Algerian Aurès Mountains." Acta Scientifica Naturalis 7, no. 2 (July 1, 2020): 15–30. http://dx.doi.org/10.2478/asn-2020-0017.
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AbstractThe objective of this study is to evaluate the antioxidant activities of six medicinal plants growing in Algerian Aurès Mountains. Total phenolic and flavonoids contents were measured using colorimetric methods, and the antioxidant capacities were evaluated using the DPPH radical scavenging and β-carotene bleaching tests. Juniperus phoenica L. had significantly the higher total phenolic compounds (53.6±3.86 mg GAE.g−1 DM) (p<0.05); followed by Romarinus officinalis L. (26.1±3.15 mg GAE.g−1 DM) and Artemisia campestris L. (20.5±1.99 mg GAE.g−1 DM). Artemisia campestris L. had significantly the higher flavonoid contents (11.1±0.56 mg QE.g−1 DM) than other studied plants (p<0.05). The best antiradical activity was observed in Thymus algeriensis extracts (EC50=11.1±0.33 µg.ml−1) and Romarinus officinalis L. (EC50=15.3±0.9 µg.ml−1). β-carotene bleaching test showed that the herbs’ phenolic compounds Antioxidant Activity (AA%) value was found in the range of 64-84%, whereas that of the standard antioxidant ascorbic acid was 51±2.4%. The present results indicate that medicinal plants from the Algerian Aurès mountains could be explored in food and pharmaceutical industries for development of natural’s antioxidant agents.
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Ben Salem, Maryem, Hanen Affes, Khaled Athmouni, Kamilia Ksouda, Raouia Dhouibi, Zouheir Sahnoun, Serria Hammami, and Khaled Mounir Zeghal. "Chemicals Compositions, Antioxidant and Anti-Inflammatory Activity of Cynara scolymus Leaves Extracts, and Analysis of Major Bioactive Polyphenols by HPLC." Evidence-Based Complementary and Alternative Medicine 2017 (2017): 1–14. http://dx.doi.org/10.1155/2017/4951937.
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Objective. Artichoke (Cynara scolymus L.) was one of the plant remedies for primary health care. The present study was focused on the determination of chemical composition, antioxidant activities, and anti-inflammatory activity and on analyzing its major bioactive polyphenols by HPLC. Methods. Artichoke Leaves Extracts (ALE) were analyzed for proximate analysis and phytochemical and antioxidant activity by several methods such as DDPH, ABTS, FRAP, and beta-carotene bleaching test. The carrageenan (Carr) model induced paw oedema in order to investigate the anti-inflammatory activity. Identification and quantification of bioactive polyphenols compounds were done by HPLC method. The oxidative stress parameters were determined; CAT, SOD, GSH, MDA, and AOPP activities and the histopathological examination were also performed. Results. It was noted that EtOH extract of ALE contained the highest phenolic, flavonoid, and tannin contents and the strongest antioxidants activities including DDPH (94.23%), ABTS (538.75 mmol), FRAP assay (542.62 umol), and β-carotene bleaching (70.74%) compared to the other extracts of ALE. Administration of EtOH extract at dose 400 mg/kg/bw exhibited a maximum inhibition of inflammation induced by Carr for 3 and 5 hours compared to reference group Indomethacin (Indo). Conclusion. ALE displayed high potential as natural source of minerals and phytochemicals compounds with antioxidant and anti-inflammatory properties.
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Tundis, Rosa, Anna Cappello, Marco Bonesi, Lorenzo Peruzzi, and Monica Loizzo. "Extracts of Different Polarity of Daphne laureola L. as Valuable Source of Antioxidant and Neuroprotective Compounds." Medical Sciences Forum 2, no. 1 (November 30, 2020): 19. http://dx.doi.org/10.3390/cahd2020-08560.
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In this study, Daphne laureola L., a European–Mediterranean species, was investigated for its antioxidant properties using different in vitro bioassays, namely 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), Ferric Reducing Activity Power (FRAP), and β-carotene bleaching assays. The acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activity using Ellman’s method was also examined. The aerial parts of D. laureola were subjected to exhaustive and subsequent macerations with solvents at different polarities, such as methanol, dichloromethane, and n-hexane. Dichloromethane extract was the most promising in DPPH and FRAP tests with IC50 values of 32.2 μg/mL and 71.5 μM Fe(II)/g, respectively. Methanol extract showed the most promising inhibition of lipid peroxidation evaluated by β-carotene bleaching test with an IC50 value of 6.6 μg/mL after 30 min of incubation. This extract was the most active against AChE (IC50 value of 56.9 μg/mL). An interesting result was obtained against BChE by the n-hexane extract, with an IC50 value of 49.7 μg/mL. In conclusion, the results suggest that D. laureola may provide a substantial source of phytochemicals, which act as natural antioxidants and acetylcholinesterase inhibitors, and may be beneficial in the treatment of Alzheimer disease.
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Bonesi, Marco, Philippe N. Okusa, Rosa Tundis, Monica R. Loizzo, Federica Menichini, Caroline Stévigny, Pierre Duez, and Francesco Menichini. "Chemical Composition, Antioxidant Properties and Anti-cholinesterase Activity of Cordia gilletii (Boraginaceae) Leaves Essential Oil." Natural Product Communications 6, no. 2 (February 2011): 1934578X1100600. http://dx.doi.org/10.1177/1934578x1100600225.
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This study aimed to investigate for the first time the chemical composition, the antioxidant properties and the acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activity of the essential oil from the leaves of Cordia gilletii De Wild (Boraginaceae). The essential oil, characterized by 23 constituents (90.1% of the total oil), was constituted by terpene derivatives (25.6%) and non-terpene derivatives (64.5%), among which aldehydes, fatty acids and alkanes were present with the percentage of 16.5%, 18.8% and 23.1%, respectively. The antioxidant activity of C. gilletii essential oil was screened by two in vitro tests: DPPH and β-carotene bleaching test. The essential oil revealed antioxidant activity with an IC50 value of 75.0 and 129.9 μg/mL on DPPH radical and β-carotene decoloration tests, respectively. Moreover, C. gilletii inhibited AChE enzyme with an IC50 value of 105.6 μg/mL.
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Bourhia, Mohammed, Kaoutar Bouothmany, Hanane Bakrim, Safaa Hadrach, Ahmad Mohammad Salamatullah, Abdulhakeem Alzahrani, Heba Khalil Alyahya, et al. "Chemical Profiling, Antioxidant, Antiproliferative, and Antibacterial Potentials of Chemically Characterized Extract of Citrullus colocynthis L. Seeds." Separations 8, no. 8 (August 4, 2021): 114. http://dx.doi.org/10.3390/separations8080114.
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Background: Citrullus colocynthis L. (C. colocynthis) is commonly known as colocynth. It belongs to the family Cucurbitaceae that is frequently used in alternative medicine in the north of Africa. The aim of the study: the present research was undertaken to investigate the chemical composition, antioxidant, antiproliferative, and antibacterial potentials of C. colocynthis seed extract. Material and methods: the chemical composition of C. colocynthis seed organic extract was characterized using gas chromatography/mass spectrometry (GC-MS). The antioxidant property was carried out using both β-carotene bleaching and DPPH assays. The antibacterial effect was effectuated using the agar disc diffusion method. The antiproliferative activity vs. human colorectal adenocarcinoma cell line (HT-29) and human breast adenocarcinoma cell line (MDA MB 231) were carried by WST-1 test. The chemical analysis showed the presence of interesting potentially bioactive compounds. The studied plant extract exhibited antioxidant potential with IC50 value of 2. 22 mg/mL (β-carotene bleaching) and 8.98 ± 0.619 mg/mL (DPPH). Concerning the antiproliferative activity, the seed extract was effective in MDA-MB-231 and HT-29 cancer cells with IC50 values 86.89 ± 3.395 and 242.1 ± 17.9 μg/mL, respectively, whilst the extract of Citrullus colocynthis seeds was non-toxic in healthy human dermal fibroblasts. Regarding the antibacterial test, the extract was effective in Gram-positive bacteria only. Conclusion: The outcome of this research indicated that the extracts from C. colocynthis seeds may compose a promising source with interesting compounds that can be used to fight cancer, free radicals damage, and bacterial infections.
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Merghem, Mounira, Saliha Dahamna, and Seddik Khennouf. "Polyphenols contents and antioxidant Activity of extracts from Leaves and flowers of Thymelaea hirsuta." Journal of Drug Delivery and Therapeutics 10, no. 4 (July 15, 2020): 108–11. http://dx.doi.org/10.22270/jddt.v10i4.4159.
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Thymelaea hirsuta is a medicinal plant, belonging to the genus Thymelaea (Thymelaeaceae) widely used in Mediterranean countries especially in Algeria. In this study, we have evaluated the total polyphenols and flavonoids contents of methanolic and aqueous extracts of Leaves and flowers of Thymelaea hirsuta as well as its antioxidant activity using the DPPH (2'2-diphenylpicrylhydrazyl) and β-carotene /linoleic acid bleaching assays. The yield of the methanolic and aqueous extract was 11, 55% and 13, 25% respectively. The total polyphenols content of the methanolic extract was 295, 22 µg GAE/ mg extract and flavonoids was 09, 40 µg QE/mg and 26, 42 µg RE/ mg extract. The total polyphenols content of the aqueous extract was 57, 95 µg GAE/ mg extract and flavonoids was 4.59 µg QE/ mg and 10.66 µg RE/ mg extract. In the DPPH assay, methanolic extract showed the higher scavenging capacity (IC50 = 0.03 ± 0.004 mg/ml), followed by aqueous extract with IC50 of 0.275 ± 0.019 mg/ml. In the test of β-carotene /linoleic acid, the percentage of inhibition was 39.1±1.33% for the aqueous extract and 41.05±2.72% for methanolic extract.
Keywords: Thymelaea hirsuta, antioxidant activity, polyphenols, DPPH scavenging, β-carotene.
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Candra Utami, Ni Putu, I. Dewa Gede Mayun Permana, and Agus Selamet Duniaji. "Pengaruh Penambahan Puree Labu Kuning (Cucurbita moschata) Terhadap Karakterisitik Siomay Udang." Jurnal Ilmu dan Teknologi Pangan (ITEPA) 10, no. 2 (June 30, 2021): 225. http://dx.doi.org/10.24843/itepa.2021.v10.i02.p06.
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This study aims to determine the effect of adding puree of pumpkin on the characteristics of shrimp dumplings and to determine the appropriate addition puree of pumpkin to produce shrimp dumplings with the best characteristics. The experimental design used in this study was a completely randomized design (CRD) consisting of 5 treatments, namely 0%, 10%, 20%, 30%, and 40%. All treatments were repeated 3 times in order to obtain 15 experimental units. The data obtained were analyzed by means of variance and if the treatment had a significant effect, then proceed with the Duncan Multipe Range Test (DMRT). The results showed that the addition of pumpkin pulp had a very significant effect on moisture content, crude fiber content, total ?-carotene, and antioxidant activity using the ?-Bleaching method. Has no significant effect on ash content, hedonic test on color, aroma, texture, taste, overall acceptance, and scoring test on color and texture. The addition of pumpkin pulp 40% had the best characteristics with water content of 61.22%, ash content of 2.59%, crude fiber content of 1.96%, total ?-carotene 0.98 mg / 100 g and antioxidant activity of 5.31%. , by the criteria of yellow color and liked, flavor liked, chewy texture and liked, taste liked and overall acceptance liked.
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Merghem, Mounira, and Saliha Dahamna. "Antioxidant Activity of Centaurium erythraea Extracts." Journal of Drug Delivery and Therapeutics 10, no. 2 (March 15, 2020): 171–74. http://dx.doi.org/10.22270/jddt.v10i2.3935.
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Phototherapy has known a great evolution all the world and some medicinal plants are importance remedies of some diseases. Centaurium erythraea is one of the medicinal plants widely used in this field. In this study, we have evaluated the total polyphenolic and the flavonoid contents of the methanolic (ME) and aqueous (AqE) extracts of the plant Centaurium erythraea as well as its antioxidant activity using the DPPH (2'2-diphenylpicrylhydrazyl), β-carotene /linoleic acid bleaching and reducing power assays. The total polyphenolic content of the methanolic extract was 35,45± 0,041 μg AGE/mg extract and flavonoids was 6,65 ± 0,060 μg QE/mg. The total polyphenolic content of the aqueous extract was 54,27 ± 0,023 μg AGE/mg extract and flavonoids was 3,275 ± 0,003 μg QE/mg. The results of the DPPH test showed a powerful antioxidant activity with a very similar IC50 for the methanolic (IC50=0.232 ± 0.002mg/ml) and aqueous (0.208 ± 0.002mg/ml) extracts. The inhibitory activity of the tow extracts in the β-carotene/linoleic acid assay was (86.781 ± 0.17%) for the methanolic extract and (77.816 ± 0.69%) for the aqueous extract. ME has the higher reducing power (IC50 = 0.35 ± 0.066 mg / ml) compared to the AqE (IC50 = 1.31 ± 0.047 mg / ml).
Keywords: Centaurium erythraea, antioxidant activity, polyphenols, DPPH scavenging, β-carotene, reducing power.
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Priyatna Putra, I. Gede, Putu Timur Ina, and Ni Made Indri Hapsari Arihantana. "Pengaruh Perbandingan Terigu Dengan Puree Labu Kuning (Cucurbita moschata) Terhadap Karakteristik Kue Nastar." Jurnal Ilmu dan Teknologi Pangan (ITEPA) 10, no. 1 (March 31, 2021): 56. http://dx.doi.org/10.24843/itepa.2021.v10.i01.p06.
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This research aims to determine the effect of the comparison of wheat flour with puree of pumpkin on the characteristics of nastar cake and to find out the right comparison of wheat flour with puree of pumpkin to produced nastar cake with the best characteristics. The experimental design used in this research was a Completely Randomized Design (CRD) with a comparison of wheat flour and puree of pumpkin consisting of 5 treatments namely 100% : 0%, 95% : 5%, 90% : 10%, 85% : 15%, and 80% : 20%. All treatments were repeated four times so that they were obtained 20 experimental units. The data obtained were analyzed by variance and if the treatment had significant effect then followed by Duncan’s Multiple Range Test. The results showed that the comparison of wheat flour and puree of pumpkin had a significant effect on the water content, total carotene content, antioxidant activity (?-carotene bleaching), color, texture, flavor, taste, and overall acceptance. Ratio of 80% wheat flour and 20% puree of pumpkin had the best characteristics with water content of 2.55%, ash content of 0.75%, ?-carotene content of 4.63 mg/100g, antioxidant activity of 1.71 mg/ml, color liked, texture rather liked, flavor liked, taste liked, and overall acceptance liked.
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Jovanović, Miloš, Zorica Drinić, Dubravka Bigović, Ana Alimpić-Aradski, Sonja Duletić-Laušević, and Katarina Šavikin. "In vitro antineurodegenerative activity and in silico predictions of blood-brain barrier penetration of Helichrysum plicatum flower extract." Lekovite sirovine, no. 40 (2020): 45–51. http://dx.doi.org/10.5937/leksir2040045j.
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This study aimed to assess the antineurodegenerative and antioxidant activity of Helichrysum plicatum flower extract, as well as to identify extract ingredients with acceptable pharmacokinetic parameters such as gastrointestinal absorption, blood-brain barrier permeation, and P-glycoprotein-mediated effusion for optimal therapeutic brain exposure. Antioxidant activity was evaluated by ABTS, FRAP, and b-carotene bleaching assays, while antineurodegenerative activity was tested using acetylcholinesterase (AChE) and tyrosinase (TYR) inhibitory activity assays. In the ABTS test, the dry extract at the highest applied concentration (500 µg/mL) showed better or similar antioxidant activity compared to the standards. In the b-carotene assay, all applied concentrations of the extract showed significantly higher activity than vitamin C. No concentration-dependent activity was observed in the AChE assay, while in the TYR assay the lowest extract concentration (100 µg/mL) showed the highest percentage of inhibition (27.92 %). Pharmacokinetic parameters of compounds were predicted by in silico SwissADME online tool in accordance by the rules of drug-likeness. According to the pharmacokinetic properties, we concluded that pentoxymethoxylated flavones may represent CNS drug candidates for further studies.
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Islam, Md Emdadul, SM Mahbubur Rahman, Md Hossain Sohrab, Rana Biswas, Mohammad Shaef Ullah, and Kazi Didarul Islam. "Concordance of antioxidant and anti-Inflammatory activity in Xylocarpus granatum (Koen)." Journal of the Bangladesh Agricultural University 17, no. 4 (December 31, 2019): 466–75. http://dx.doi.org/10.3329/jbau.v17i4.44607.
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Xylocarpus granatum, a common mangrove plant is traditionally used for the treatment of diarrhoea, cholera, fever, dyslipidemia, inflammation, etc. The present study was carried out to evaluate the antioxidant and anti-inflammatory potential of the ethanolic extract of X. Granatum by various in vitro methods such as 1,1-diphenyl-2-picryl-hydrazil (DPPH) free radical scavenging assay, reducing power assay, ferric reducing antioxidant power (FRAP) and β-carotene bleaching inhibition assay. Total phenolic and flavonoid content were determined. Anti-inflammatory activity was evaluated by in vitro human RBC membrane stabilizing assay and in vivo mice paw edema test. Ethanolic leaf extract (S2) of X. granatum showed significant scavenging effect on DPPH scavenging with a value of IC50 165.95µg/ml. In addition, it showed significant reducing potential with a value of 59.04 mM of ferrous equivalent per ml in FRAP assay and in reducing power assay the EC50 value was determined as 241.61μg/ml . The ethanolic leaf extracts exhibited 72.3% β-carotene bleaching inhibition. The total phenolic and flavonoid content of the extract were 66μg/ml gallic acid equivalent and 47.66μg/ml quercetin equivalent per gram of dry extract, respectively. The extract also exhibited 52.63% and 51.05% protection of RBC membrane in hypotonicity and heat induced lysis inhibition, respectively. Significant reduction of mice paw edema (36.34% in 20 μg/kg bw concentration) was observed in the extract. The results revealed that the leaf extract of X. granatum possesses strong antioxidant and anti-inflammatory potential.
J Bangladesh Agril Univ 17(4): 466–475, 2019
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Guemmaz, Thoraya, Lekhmici Arrar, and Abderrahmene Baghiani. "Total Phenolic Contents and Antioxidant Properties of Algerian Alkanna tinctoria aerial part Extracts." Journal of Drug Delivery and Therapeutics 10, no. 5 (September 15, 2020): 39–44. http://dx.doi.org/10.22270/jddt.v10i5.4349.
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Objective: Evaluation of the polyphenols, flavonoids and tannins contents of different aqueous extracts of the aerial part of Alkanna tinctoria and their antioxidant activities.
Methodology: Three extracts; decoction, macerate and infusion of the aerial part of Alkanna tinctoria were prepared. The content of total polyphenols, flavonoids and tannins of the extracts was carried out and antioxidant activity was assessed using DPPH (2,2’-diphenyl-1-picryl hydrazyl), β-carotene bleaching and iron chelation tests.
Results : The results showed that Infusion (Inf) was the richest in total polyphenols (189.66 ± 30.77 mg EGA / g of dried extract) and flavonoids (26.80 ± 8.37 mg EQ / g of dried extract). While the content of tannins is almost the same for Dec and Inf (40.27 ± 15.00, 40.25 ± 18.27 mg ETA / g of dried extract). Moreover, the two extracts Inf (IC50 of 0.09 ± 0.0015 mg / ml) and Dec (IC50 of 0.17 ± 0. 031 mg / ml) showed a very strong anti-radical activity towards the radical DPPH. In the β-carotene bleaching test, Inf and Dec showed a significant inhibitory activity of peroxidation with values of 100% and 99.8% and are more effective compared to BHT (98.15%). Dec and Mac showed strong chelating activity with IC50s of 0.003 ± 0.0002 mg / ml and 0.005 ± 0.00008 mg / ml respectively. These chelating activities remain higher than EDTA.
Conclusion: It may be concluded that the extracts of the aerial part of Alkanna tinctoria exhibit significant both content of polyphenols and antioxidant activity.
Keywords: Alkanna tinctoria, polyphenols, flavonoids, antioxidant activity.
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Sun, Zongxi, Ruiqiang Su, Jianwei Qiao, Zhiquan Zhao, and Xinsheng Wang. "Flavonoids Extraction fromTaraxacum officinale(Dandelion): Optimisation Using Response Surface Methodology and Antioxidant Activity." Journal of Chemistry 2014 (2014): 1–7. http://dx.doi.org/10.1155/2014/956278.
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The Box-Behnken design combined with response surface method was employed to optimize ultrasonic-assisted extraction of flavonoids fromTaraxacum officinale. The optimized results showed that the highest extraction yield with ultrasonic-assisted extraction could reach 2.62% using 39.6% (v/v) ethanol and 59.5 : 1 (mL/g) liquid-solid ratio for 43.8 min. The crude extract was then purified by HPD-100 macroporous adsorption resin, and the flavonoids content in the purified extract increased to 54.7%. The antioxidant activity of the purified flavonoids was evaluatedin vitroby scavenging capacity of ABTS or DPPH,β-carotene bleaching, and FTC test. The knowledge obtained from this study should be useful to further develop and apply this plant resource.
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Mokhnache, Kamel, Soraya Madoui, Hanane Khither, El-Khamsa Soltani, Noureddine Charef, and Arrar Lekhmici. "Synthesis, Characterization, Hydrolytic Cleavage, and Biological Activity Studies of 2-[(1e)-N-{2-[(2-{(Z)-[1-(2-Hydroxyphenyl)Ethylidene] Amino}Ethyl)Amino]Ethyl}Ethanimidoyl]Phenol." Journal of Drug Delivery and Therapeutics 9, no. 5-s (October 15, 2019): 25–29. http://dx.doi.org/10.22270/jddt.v9i5-s.3621.
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A Schiff base ligand 2-[(1E)-N-{2-[(2-{(Z)-[1-(2-hydroxyphenyl) ethylidene] amino}ethyl)amino]ethyl} ethanimidoyl]phenol L was hydrolyzed by copper cation which lead to formation of 8,8-dichloro-2H,3H,5H,6H-1,3-diaza-2-cupracyclopenta[1,3-a]1,3-diaza-2-cupracyclopentane hydrate (Complex), characterized by UV, IR, Powder XRD and by elemental analysis. In vitro antioxidant and anticoagulant, activities of L were evaluated. Antioxidant potential of L was assessed by DPPH scavenging, β-carotene bleaching test, hydroxyl radical scavenging method, ABTS radical scavenging test, and by reducing power test. In vitro anticoagulant effect of L at the 84 µg/mL; showed the maximum prolongation of plasma recalcification time which is comparable with that of the anticoagulant drug; heparin. In conclusion, results of the present investigation indicate that the ligand L can be a potential anticoagulant agent.
Keywords: Schiff base; Antioxidant; Free radicals; Anticoagulant.
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Chebbah, Kaoutar, Eric Marchioni, Djamel Sarri, Ahmed Menad, Ratiba Mekkiou, Ramdane Seghiri, Ouahiba Boumaza, Samir Benayache, and Fadila Benayache. "HPLC-UV profile of Genista ulicina Spach. (Fabaceae) extracts and in vitro antioxidant activity." International Journal of Phytomedicine 8, no. 3 (November 9, 2016): 398. http://dx.doi.org/10.5138/09750185.1863.
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<p>To perform a qualitative and quantitative analysis of the phenolic and flavonoid contents and evaluate the antioxidant activity of ethyl acetate (EtOAc) and <em>n</em>-butanol (<em>n</em>-BuOH) extracts of the aerial parts of <em>Genista ulicina </em>Spach. from Algeria.<strong> </strong>The qualitative analysis of plant extracts was carried out by RP-HPLC using UV detector, whereas the quantification of total phenolic and flavonoid contents was completed according to the Folin-Ciocalteu procedure and aluminium chloride colorimetric method respectively. To evaluate the extract's antioxidant activity, Two in vitro antioxidant tests were employed: DPPH and β-carotene bleaching assay. The HPLC/DAD chromatogram showed several peaks indicating the presence of phenolic acids, flavonoids and isoflavonoids in both extracts. The total phenolic content (TPC) ranged from 62.56 and 50.45 mgGAE/g extract, while the total flavonoids content varied between 53.1 and 48.4 mgQE/g extract for EtOAC and <em>n</em>-BuOH respectively. EtOAc extract showed a maximum inhibition value (78.15%) at 150µg/mL using DPPH test and highest antioxidative power (82.42%) using β-carotene bleaching assay comparing with standards. The HPLC-UV analysis showed the richeness of both extracts in phenolic and flavonoid contents. The EtOAc<em> </em>extract exhibited good antioxidant activities comparing to the <em>n</em>-BuOH extract. Thus <em>Genista ulicina</em> could be indicated as a plant of phytopharmaceutical importance.<strong></strong></p>
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Vasconcelos, Mayron Alves, Francisco Vassiliepe Sousa Arruda, Daniel Barroso de Alencar, Silvana Saker-Sampaio, Maria Rose Jane Ribeiro Albuquerque, Hélcio Silva dos Santos, Paulo Nogueira Bandeira, et al. "Antibacterial and Antioxidant Activities of Derriobtusone A Isolated fromLonchocarpus obtusus." BioMed Research International 2014 (2014): 1–9. http://dx.doi.org/10.1155/2014/248656.
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This study evaluated the effect of derriobtusone A, a flavonoid isolated fromLonchocarpus obtusus, on two important pathogenic bacteria,Staphylococcus aureusandEscherichia coli, as well as its antioxidant activity and toxicity. Planktonic growth assays were performed, and the inhibition of biofilm formation was evaluated. In addition, antioxidant activity was assessed by DPPH radical scavenging assay, ferrous ion chelating assay, ferric-reducing antioxidant power assay, andβ-carotene bleaching assay. Toxicity was evaluated by the brine shrimp lethality test. Results showed that derriobtusone A completely inhibited the planktonic growth ofS. aureusat 250 and 500 μg/mL; however, it did not have the same activity onE. coli. Derriobtusone A reduced the biomass and colony-forming unit (cfu) ofS. aureusbiofilm at concentrations of 250 and 500 μg/mL. In various concentrations, it reduced the biofilm biomass ofE. coli, and, in all concentrations, it weakly reduced the cfu. Derriobtusone A showed highly efficient antioxidant ability in scavenging DPPH radical and inhibitingβ-carotene oxidation. The compound showed no lethality toArtemiasp. nauplii. In conclusion, derriobtusone A may be an effective molecule againstS. aureusand its biofilm, as well as a potential antioxidant compound with no toxicity.
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ALENCAR, DANIEL B. DE, SUZETE R. DA SILVA, KELMA M. S. PIRES-CAVALCANTE, REBECA L. DE LIMA, FRANCISCO N. PEREIRA JÚNIOR, MÁRCIA B. DE SOUSA, FRANCISCO A. VIANA, et al. "Antioxidant potential and cytotoxic activity of two red seaweed species, Amansia multifida and Meristiella echinocarpa, from the coast of Northeastern Brazil." Anais da Academia Brasileira de Ciências 86, no. 1 (March 2014): 251–63. http://dx.doi.org/10.1590/0001-37652014116312.
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Natural antioxidants found in marine macroalgae are bioactive compounds known to play an important role in the prevention of diseases associated with aging cells protecting them against the oxidative damage. The purpose of this study was to evaluate the antioxidant and cytotoxic activity of ethanolic extracts of two species of red seaweeds, Amansia multifida and Meristiella echinocarpa. In vitro antioxidant activity was determined by DPPH radical scavenging assay, ferric-reducing antioxidant power (FRAP) assay, ferrous ion chelating (FIC) assay, β-carotene bleaching (BCB) assay and total phenolic content (TPC) quantification. Cytotoxicity was evaluated with the brine shrimp Artemia sp. lethality test. The TPC values observed in the present study indicated that both species A. multifida and M. echinocarpa are rich in phenolic compounds, reaching values of 45.40 and 28.46 mg gallic acid equivalent (GAE) g−1 of ethanolic extract, respectively. DPPH radical scavenging and ferrous ion chelating showed values of 60% and 17%, respectively. Both seaweed extracts inhibited β-carotene oxidation by approximately 40%. None of the algal extracts were potentially cytotoxic. The results have showed that extracts of both species of marine red algae exhibit antioxidant potential and low toxicity. They are sources of natural antioxidant compounds.
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Zitouni, Hafida, Lahcen Hssaini, Rachida Ouaabou, Manuel Viuda-Martos, Francisca Hernández, Sezai Ercisli, Said Ennahli, Zerhoune Messaoudi, and Hafida Hanine. "Exploring Antioxidant Activity, Organic Acid, and Phenolic Composition in Strawberry Tree Fruits (Arbutus unedo L.) Growing in Morocco." Plants 9, no. 12 (November 30, 2020): 1677. http://dx.doi.org/10.3390/plants9121677.
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This study aimed to explore the main biochemical components and the antioxidant capacity of five strawberry tree fruits using three antioxidant essays within the ecotypic comparison scheme, to find out the most valuable fruit presenting disease-preventing properties. Total phenols, total flavonoids, total anthocyanins, antioxidant activity (DPPH, ABTS, and β-Carotene bleaching assays), pH, titratable acidity, soluble solids, and moisture content were investigated in five strawberry tree genotypes belonging to several areas in Morocco. Phenolic compounds were also identified using high performance chromatography (HPLC), with a diode array detector (DAD). High significant differences (p ˂ 0.05) were revealed among the examined genotypes regarding their total phenols (25.37–39.06 mg gallic acid equivalents (GAE)/g Dry weight (DW), total flavonoids (3.30–7.07 mg RE/g Dry weight (DW), total anthocyanins (0.15–0.64 mg cya-3-glu/100g Dry weight (DW), pH (2.44–3.92), titratable acidity (0.65–1.01 g malic acid/100g Fresh weight (FW), and soluble solids (14.83–18.53%). The average radical scavenging capacity, assessed using three methods, exhibited the following concentration ranges: 3.33–21.08, 2.25–19.58, and 1.08–13 mg Ascorbic Equivalent (AAE/g Dry weight(DW) for the DPPH scavenging test, ABTS, and β-carotene bleaching, respectively. Seventeen phenolic compounds were identified in sampled cultivars. Gallocatechol and catechin were found to be the major phenolic compounds. The correlation matrix revealed significant correlations among investigated variables, particularly ABTS and DPPH. The principal component analysis showed that the first three components formed 90.25% of the total variance. The following variables: chlorogenic acid, ellagic acid derivative, ellagic acid, rutin, and cyanidin−30.5-diglucoside, were the most involved in the total variance. The results revealed highly promising physico-biochemical profiles within the studied strawberry tree genotypes.
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Batista, Daniela, Pedro L. Falé, Maria L. Serralheiro, Maria-Eduarda Araújo, Catarina Dias, Isabel Branco, Clara Grosso, et al. "Phytochemical Characterization and Biological Evaluation of the Aqueous and Supercritical Fluid Extracts from Salvia sclareoides Brot." Open Chemistry 15, no. 1 (April 29, 2017): 82–91. http://dx.doi.org/10.1515/chem-2017-0011.
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AbstractPlants belonging to the genus Salvia (Lamiaceae) are known to have a wide range of biological properties. In this work, extracts obtained from the aerial parts of Salvia sclareoides Brot. were evaluated to investigate their chemical composition, toxicity, bioactivity, and stability under in vitro gastrointestinal conditions. The composition of the supercritical fluid extract was determined by GC and GC-MS, while the identification of the infusion constituents was performed by HPLC-DAD and LC-MS. The in vitro cytotoxicity of both extracts (0-2 mg/mL) was evaluated in Caco-2 cell lines by the MTT assay. The anti-inflammatory and anticholinesterase activities were determined through the inhibition of cyclooxygenase-1 and acetylcholinesterase enzymes, while β-carotene/linoleic acid bleaching test and the DPPH assays were used to evaluate the antioxidant activity. The infusion inhibited cyclooxygenase-1 (IC50 = 271.0 μg/mL), and acetylcholinesterase (IC50 = 487.7 μg/ mL) enzymes, also demonstrated significant antioxidant properties, as evaluated by the DPPH (IC50 = 10.4 μg/mL) and β-carotene/linoleic acid (IC50 = 30.0 μg/mL) assays. No remarkable alterations in the composition or in the bioactivities of the infusion were observed after in vitro digestion, which supports the potential of S. sclareoides as a source of bioactive ingredients with neuroprotective, anti-inflammatory and antioxidant properties.
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Kazemi, Mohsen. "Effects of nano-ferric oxide on quality, quantity and antioxidant properties of essential oil components in Carum copticum (L.) Link." Bangladesh Journal of Botany 44, no. 3 (October 13, 2018): 367–72. http://dx.doi.org/10.3329/bjb.v44i3.38541.
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The effect of exogenous application of nano-ferric oxide (50 and 100 mg/l) in early flowering stage on components of essential oils (EOs) of Carum copticum (L.) Link was evaluated. EO extracted by hydrodistillation from Iranian C. copticum was characterized by means of GC/MS. The nano-ferric oxide application increased limonene, γ- terpinene, cis-limonene oxide, thymol, carvacrol and decreased α-thujene, α-pinene, β-pinene and p-cymene concentration. The EO was also subjected to evaluation for antioxidant properties. Thymol, γ-terpinene and carvacrol possessed the highest antioxidant properties among the major components. C. copticum EO exhibited a higher activity in each antioxidant system with a special attention to β-carotene bleaching test and reducing power. The TLC-bioautography screening and fractionation resulted in the separation of the main antioxidant compounds which were identified as thymol, γ-terpinene and carvacrol.
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Khebri, Souad, Naima Benkiki, Mouloud Yahia, and Hamada Haba. "Phytochemical Profile, Antimicrobial and Antioxidant Activities of Cistus clusii Essential oil From Algeria." Current Bioactive Compounds 16, no. 2 (April 23, 2020): 109–14. http://dx.doi.org/10.2174/1573407214666180802125128.
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Objective: In this work, we investigated the chemical composition and the antioxidant and antimicrobial activities of the Algerian Cistus clusii Dunal essential oil. Methods: Cistus clusii essential oil has been extracted using hydrodistillation method and characterized by GC/MS. The antioxidant activity was evaluated by two different methods DPPH free radical scavenging, and β-carotene bleaching test. The antimicrobial activity was carried out using disc diffusion method against reference strains; Pseudomonas aeruginosa (ATCC 27865), Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 25923), clinical bacterial (Klebsiella pneumoniae, Proteus mirabilis, Staphylococcus aureus) and fungal (Candida albicans) isolates. Results: The chemical profile of the oil showed 31 compounds. This profile was dominated by the presence of monoterpene hydrocarbons (78.4%), oxygenated derivatives (18.8%) and sesquiterpene hydrocarbons as minor constituents of this essential oil (1.9%). The major constituents were Camphene (20.6%), γ-Terpinene (16.5%), Sabinene (12.8%), and Terpineol-4 (13.5%). Other compounds were found in moderately good amounts like α-Terpinene (8.9%), α-Thujene (5.9%), α-Pinene (4.2%), and α- Terpinolene (3.1%). The results of the antimicrobial activity showed that the highest activity was observed against C. albicans and it was higher than positive control Fluconazole. The essential oil exhibited a strong antioxidant activity, especially in the β-carotene system. Conclusion: This study indicates that Cistus clusii essential oil showed potent antioxidant and moderate antimicrobial activities which could be used to explain the use of this species in traditional medicine.
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Bencheikh, Noureddine, Mohamed Bouhrim, Ilyass Alami Merrouni, Salima Boutahiri, Loubna Kharchoufa, Mohamed Addi, Duangjai Tungmunnithum, et al. "Antihyperlipidemic and Antioxidant Activities of Flavonoid-Rich Extract of Ziziphus lotus (L.) Lam. Fruits." Applied Sciences 11, no. 17 (August 24, 2021): 7788. http://dx.doi.org/10.3390/app11177788.
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Ziziphus lotus (L.) Lam. (Z. lotus) is a medicinal plant species that is widely distributed throughout the Mediterranean basin. Moroccans traditionally use it to treat many illnesses thanks to its beneficial medicinal properties. The purpose of this study is to assess the anti-hyperlipidemic and antioxidant activities of a flavonoid-rich aqueous extract of Z. lotus fruits (ZLF). The 2-2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay and the β-carotene bleaching test were used to determine the antioxidant activity of ZLF. The anti-hyperlipidemic effect of the aqueous extract of ZLF (200 and 400 mg/kg) was evaluated in albino mice exposed to the chronic high-fat diet, based on lipid profile, blood sugar, and changes in growth performance. The results showed that the aqueous extract of ZLF rich in flavonoids (2242.89 ± 25 µg QE (quercitin equivalent)/mg), and has a considerable antioxidant power against DPPH radicals (IC50 = 116 ± 0.02 µg/mL) and β-carotene oxidation. The aqueous extract of ZLF also showed a significant anti-hyperlipidemic effect by improving abnormal changes in lipid profile and blood glucose levels in albino mice exposed to a chronic high-fat diet. Our findings suggest that the anti-hyperlipidemic activities of ZLF aqueous extract are correlated with its flavonoid content and antioxidant activity. Therefore, the aqueous extract of ZLF could be an essential therapeutic candidate for hyperlipidemia patients, thanks to its richness in bioactive molecules.
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Belmehdi, Omar, Badia Douhri, Abdelhakim Bouyahya, Yousif Laghmouchi, Nadia S. Senhaji, and Jamal Abrini. "Phenolic Content, Antibacterial and Antioxidant Activities of Moroccan propolis." Current Bioactive Compounds 15, no. 6 (January 23, 2020): 696–705. http://dx.doi.org/10.2174/1573407214666180530100947.
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Background:Propolis contains compounds with antibacterial and antioxidant capacities, such as phenolic compounds and flavonoids. The aim of this study was to investigate the antibacterial and antioxidant effects of six propolis samples from northern Morocco and to determine their content of polyphenols and flavonoids.Methods:The total polyphenols and flavonoids of Ethanol Extracts (EEP) were evaluated by colorimetric assays. To test the antibacterial activity, the well diffusion and micro-dilution methods were adopted. The kinetic of bacterial growth in the presence of EEP was tested. The antioxidant activity was evaluated using three techniques: reducing power, β-carotene bleaching test, and DPPH scavenging capacity assay.Results:The EEPs were found to have high total phenolic content (65.09 - 122.00 mg GAE/g) and flavonoids (41.95 - 50.44 mg QE/g). The screening of the antibacterial activity showed that all tested bacteria were sensitive to EEP except Escherichia coli. The inhibitory zones varied from 11.50 ± 0.70 to 32.00 ± 0.70 mm, and MIC and MBC ranged from 0.07 to 5 mg/mL and 0.07 to >5 mg/mL, respectively. The antioxidant activity was proportional to the phenolic content of propolis. The IC50 varied from 48.70 to 156.00 mg/mL and the RAA% ranged from 75.07 to 96.68 %.
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M. Afonso, Alexandra, Joana Gonçalves, Ângelo Luís, Eugenia Gallardo, and Ana Paula Duarte. "Evaluation of the In Vitro Wound-Healing Activity and Phytochemical Characterization of Propolis and Honey." Applied Sciences 10, no. 5 (March 7, 2020): 1845. http://dx.doi.org/10.3390/app10051845.
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Honey and propolis are natural substances produced by Apis mellifera that contain flavonoids, phenolic acids, and several other phytochemicals. The aim of this study was to phytochemically characterize three different types of honey and propolis, both separately and mixed, and to evaluate their wound-healing activity. Total phenolic compounds and flavonoids were determined using the Folin–Ciocalteu’s and aluminum chloride colorimetric methods, respectively. The antioxidant activity was evaluated by both the DPPH free radical scavenging assay and β-carotene bleaching test, and the anti-inflammatory activity was determined by a protein denaturation method. To evaluate the wound-healing activity of the samples, NHDF cells were subjected to a wound scratch assay. The obtained results showed that dark-brown honey presents a higher concentration of phenolic compounds and flavonoids, as well as higher antioxidant and anti-inflammatory activities. Propolis samples had the highest concentrations in bioactive compounds. Examining the microscopic images, it was possible to verify that the samples promote cell migration, demonstrating the wound-healing potential of honey and propolis.
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Mouffouk, Soumia, Chaima Mouffouk, Chawki Bensouici, and Hamada Haba. "In vitro Cytotoxic Effect, Hemolytic, and Antioxidant Activities of the Algerian Species Nonea vesicaria Rchb." Current Bioactive Compounds 16, no. 8 (November 10, 2020): 1197–204. http://dx.doi.org/10.2174/1573407216666200109120431.
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Objective: The aim of this study is the estimation of total phenolic and flavonoid contents and the evaluation of cytotoxic, hemolytic and antioxidant activities of the methanolic extract obtained from the species Nonea vesicaria (L.) Rchb. Methods: The total phenolic and flavonoid contents were quantified by Folin-Ciocalteu and trichloroaluminum methods, respectively. The cytotoxic effect was tested by Brine shrimp lethality assay and the hemolytic activity was assessed by spectrophotometric test on human erythrocytes. Moreover, the antioxidant activity was determined by seven different techniques. Results: The phytochemical screening revealed the presence of many classes of secondary metabolites, a moderate level of polyphenols, and a low content of flavonoids. The methanolic extract showed a significant cytotoxic effect with a value of LC50 at 35.7±0.5 μg/mL and induced hemolysis in a dosedependent manner with a value of EC50 at 175.6±0.08 μg/mL. The results of antioxidant activities indicated an important effect on nonpolar systems especially in ferric thiocyanate test and β-carotene bleaching inhibition assay. Conclusion: The methanolic extract of N. vesicaria could constitute an important source of antioxidant and cytotoxic compounds but prudent use is recommended in order to reduce the adverse effects related to the possible hemolysis.
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EL Moussaoui, Abdelfattah, Mohammed Bourhia, Fatima Zahra Jawhari, Ahmad Mohammad Salamatullah, Riaz Ullah, Ahmed Bari, Hafiz Majid Mahmood, et al. "Chemical Profiling, Antioxidant, and Antimicrobial Activity against Drug-Resistant Microbes of Essential Oil from Withania frutescens L." Applied Sciences 11, no. 11 (June 2, 2021): 5168. http://dx.doi.org/10.3390/app11115168.
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This work was conducted to study the chemical composition, antioxidant, antibacterial, and antifungal activities of essential oil and hydrolat from Withania frutescens. The essential oil was extracted by hydrodistillation. The chemical characterization was performed using gas chromatography-mass spectrometry (GC/MS). The antioxidant activity was studied using four different assays (DPPH, TAC, FRAP, and β-carotene bleaching). The antibacterial activity test was carried out on multidrug-resistant bacteria including Gram-negative and Gram-positive strains. Antifungal activity was tested on Candida albicans and Saccharomyces cerevisiae. The yield of essential oil (EO) obtained by hydrodistillation of W. frutescens was 0.31% majorly composed of camphor, α-thujone, carvacrol, and thymol. Regarding the antioxidant activities, the concentration of the sample required to inhibit 50% of radicals (IC50) of EO and hydrolat were 14.031 ± 0.012 and 232.081 ± 3.047 µg/mL (DPPH), 4.618 ± 0.045 and 8.997 ± 0.147 µg/mL (FRAP), 0.091 ± 0.007 and 0.131 ± 0.004 mg AAE/mg (TAC), 74.141 ± 1.040% and 40.850 ± 0.083% (β-carotene), respectively. Concerning the antibacterial activity of essential oil and hydrolat, the minimum inhibitory concentration (MIC) values found were 0.006 ± 0.001 and 6.125 ± 0.541 µg/mL (Escherichia coli 57), 0.003 ± 0.001 and 6.125 ± 0.068 µg/mL (Klebsiella pneumoniae), 0.001 ± 0.0 and 6.125 ± 0.046 µg/mL (Pseudomonas aeruginosa) and 0.012 ± 0.003 and 6.125 ± 0.571 µg/mL (Staphylococcus aureus), respectively. MIC values of essential oil and hydrolat vs. both C. albicans and S. cerevisiae were lower than 1/20,480 µg/mL. Based on the findings obtained, essential oils of Withania frutescens can be used as promising natural agents to fight free radical damage and nosocomial antibiotic-resistant microbes.
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Silva, Fabrício Souza, Pedro Modesto Nascimento Menezes, Pedro Guilherme Souza de Sá, André Luís de Santana Oliveira, Eric Alencar Araújo Souza, Vinicius Martins Bamberg, Henrique Ribeiro de Oliveira, et al. "Pharmacological Basis for Traditional Use of theLippia thymoides." Evidence-Based Complementary and Alternative Medicine 2015 (2015): 1–10. http://dx.doi.org/10.1155/2015/463248.
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The aim of this study was to evaluate crude extracts and fractions from leaves and stems ofLippia thymoidesand to validate their use in folk medicine.In vitroantioxidant and antimicrobial activities andin vivowound healing in rats, baker yeast-induced fever in young rats, and acute oral toxicity in mice assays were realized. The crude extracts and their dichloromethane and ethyl acetate fractions had potent radical-scavenging activity against the DPPH but were not effective in theβ-carotene bleaching method. The dichloromethane fraction from the leaves extract showed the broadest spectrum of activity againstS. aureus,B. cereus, andC. parapsilosis. The animals treated with crude extracts showed no difference in wound healing when compared with the negative control group. The crude extract from leaves (1200 mg/kg) has equal efficacy in reducing temperature in rats with hyperpyrexia compared to dipyrone (240 mg/kg) and is better than paracetamol (150 mg/kg). In acute toxicity test, crude extract of leaves fromLippia thymoidesexhibited no mortality and behavioral changes and no adverse effects in male and female mice. This work validates the popular use ofLippia thymoidesfor treating the wound and fever, providing a source for biologically active substances.
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El Kamli, T., M. El Hamdani, N. Eloutassi, F. Errachidi, R. Chabir, and A. Bour. "Chemical Composition, Antioxidant, and Antimicrobial Activities of Rosmarinus officinalis Essential Oil From Moroccan Middle Atlas." Phytothérapie 18, no. 3-4 (September 12, 2019): 162–68. http://dx.doi.org/10.3166/phyto-2019-0128.
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This study was designed to evaluate antioxidant and antibacterial activities of essential oils from Rosmarinus officinalis obtained by three different extraction methods: DA: Artisanal distillation; the essential oils were obtained directly from small cooperatives using a very traditional (nonindustrial) method and based on the technique of Steam distillation; DI: Industrial distillation which is also based on steam distillation; and DC: hydrodistillation through Clevenger apparatus laboratory. The chemical analyses were carried out with gas chromatography/mass spectrometry (GC/MS); they identified 16 components representing more than 99.89% of the essential oil and indicate that the chemotype is 1,8-Cineole and varies according to the method used (DI: 49.09%, DA: 42.12%, and DC: 53.21%). The antioxidant activity was evaluated by the β-carotene bleaching test measuring percent inhibition of peroxidation in linoleic acid system. The disc diffusion and modified resazurin microtiter-plate assays were used, respectively, to evaluate the inhibition zones (IZ) and minimum inhibitory concentration (MIC) of Rosmarinus officinalis essential oil. In general, Rosmarinus officinalis L. essential oil showed a lower antioxidant and antimicrobial activity than 1,8-Cineole the major component of the essential oil.
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González, Ana M., María I. Tracanna, Sara M. Amani, Carola Schuff, Miguel J. Poch, Horacio Bach, and César A. N. Catalán. "Chemical Composition, Antimicrobial and Antioxidant Properties of the Volatile Oil and Methanol Extract of Xenophyllum Poposum." Natural Product Communications 7, no. 12 (December 2012): 1934578X1200701. http://dx.doi.org/10.1177/1934578x1200701230.
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The essential oil and methanol extract of northwestern Argentina medicinal plant Xenophyllum poposum, collected in Catamarca province, were investigated. GC and GC-MS analyses of the essential oil identified 56 compounds accounting for 92.9%. The main components of the oil were δ-cadinene (16.5%), 6-hydroxytremetone (14.7%), epi-α-cadinol (12.0%), α-cadinol (8.8%), γ-cadinene (7.5%), 1- epi-cubenol (4.2%) and α-muurolene (3.0%). The essential oil exhibited antibacterial activities against five pathogenic strains as well as antifungal activities against two pathogenic fungi. The methanol extract showed antibacterial activity against two strains of Staphylococcus aureus and two pathogenic fungal strains. The main components isolated from the methanol extract were the antifungal 4-hydroxy-3-(isopenten-2-yl)-acetophenone, 6-hydroxytremetone, and tremetone. 6-Hydroxytremetone showed activity against all the fungal strains and one of the S. aureus strains assayed. Antioxidant and radical-scavenging properties of the methanol extract and essential oil were determined using the 2,2′-diphenyl-1-picrylhydrazyl assay and β-carotene bleaching (BCB) test. The methanol extract and the essential oil showed, respectively, moderate and weak antioxidant activity when compared to butylated hydroxytoluene.
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Marrelli, Mariangela, Fabrizio Araniti, Maria Rosa Abenavoli, Giancarlo Statti, and Filomena Conforti. "Potential Health Benefits of Origanum heracleoticum Essential Oil: Phytochemical and Biological Variability among Different Calabrian Populations." Natural Product Communications 13, no. 9 (September 2018): 1934578X1801300. http://dx.doi.org/10.1177/1934578x1801300921.
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The potential health benefits of the essential oils of six different populations of Origanum heracleoticum L. from Calabria (Italy) were assessed, together with the evaluation of the influence of the site of collection on their metabolic profile and biological activity. Different terpenoids were identified, being thymol, carvacrol and o-cymene among the most abundant ones. Hierarchical cluster analysis allowed the identification of two main groups, which were discriminated by the altitude of collection. The antioxidant activity was tested by means of two in vitro assays: DPPH and β-carotene bleaching test. Samples EO-4 and EO-3 were the most active in protecting linoleic acid from peroxidation, with IC50 values of 4.00 and 4.68 μg/mL after 30 minutes of incubation. The anti-inflammatory potential was assessed through the evaluation of the capacity to inhibit NO production in lipopolysaccharide (LPS)-induced murine macrophage RAW 264.7 cell line. All essential oils induced a dose-depending inhibitory effect. An excellent activity was demonstrated for sample EO-4, followed by sample EO-3 (IC50 values equal to 32.77 and 49.48 μg/mL, respectively). Observed biological properties were correlated to the phytochemical content of analyzed samples.
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Lima-Saraiva, Sarah Raquel Gomes de, Fernanda Granja da Silva Oliveira, Raimundo Gonçalves de Oliveira Junior, Camila de Souza Araújo, Ana Paula de Oliveira, Alessandra Gomes Marques Pacheco, Larissa Araújo Rolim, Elba Lúcia Cavalcanti de Amorim, Francine Celise Siqueira César, and Jackson Roberto Guedes da Silva Almeida. "Chemical Analysis and Evaluation of Antioxidant, Antimicrobial, and Photoprotective Activities of Schinopsis brasiliensis Engl. (Anacardiaceae)." Scientific World Journal 2017 (2017): 1–10. http://dx.doi.org/10.1155/2017/1713921.
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Schinopsis brasiliensis Engl. is a native plant of Caatinga which has high concentrations of compounds capable of absorbing ultraviolet light, suggesting its potential application for the development of sunscreen preparations. After its identification and collection, this vegetable drug was submitted to a physicochemical analysis through the preparation of ethanolic extract. The phytochemical screening and analysis of extracts were carried out by high-performance liquid chromatography (HPLC) evaluation. The antioxidant activity of the extract was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and β-carotene bleaching test. Inhibitory hemolytic activity and morphological deformation of erythrocytes induced by H2O2 were also demonstrated and the antimicrobial activity was analyzed by the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) method. For the in vitro determination of the sun protection factor (SPF), the spectrophotometric method was used. From the analyses carried out with this species, this plant showed significant results for the antioxidant and antimicrobial activities, as well as sunscreen action. Important flavonoids were identified. These data are an important step for the development of new photoprotective cosmetic with Caatinga species, revealing importance and representing another incentive for the preservation of the species involved and analyzed in the study.
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Plastina, Pierluigi, Rosa Tundis, Chiara La Torre, Vincenzo Sicari, Angelo Maria Giuffré, Alessandro Neri, Marco Bonesi, et al. "The addition of Capsicum baccatum to Calabrian monovarietal extra virgin olive oils leads to flavoured olive oils with enhanced oxidative stability." Italian Journal of Food Science 33, no. 1 (February 6, 2021): 61–72. http://dx.doi.org/10.15586/ijfs.v33i1.1937.
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This study aimed to evaluate the influence of Capsicum baccatum L. Aji Angelo and Bishop crown cultivars to the quality parameters of flavoured olive oils (FOOs) obtained by the addition of both fresh and dried pepper powders (1%) to Dolce di Rossano and Roggianella monovarietal extra virgin olive oils (EVOOs). First, pepper extracts were investigated for their total phenolic, flavonoid, carotenoid content as well as phenolic acids, fatty acid profile, and vitamin C and E content. In order to evaluate the impact of both fresh and dried peppers on the oxidative stability of FOOs, the Rancimat test was applied. 2,2-Azinobis(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), ?-carotene bleaching (B-CB) and ferric reducing antioxidant power (FRAP) assays were used to investigate the antioxidant potential. Bishop crown dried extracts showed the highest phenolic, carotenoid and vitamin content, whereas Aji Angelo had the highest amount of capsaicinoids. Among EVOOs, Roggianella EVOO showed the highest antioxidant activity as well as the highest induction time (39.6 h). Remarkably, FOO obtained by the addition of Bishop crown dried pepper extract to Roggianella EVOO showed a higher induction time (44.9 h) with respect to the corresponding EVOO.
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Zahaf, Cherifa, Djilali Achour, Meriem Mokhtar, and Moussa Brada. "Chemical composition, antibacterial and antioxidant activities of Juniperus oxycedrus essential oils from North Western Algeria." South Asian Journal of Experimental Biology 7, no. 2 (January 9, 2018): 74–83. http://dx.doi.org/10.38150/sajeb.7(2).p74-83.
Full textAbstract:
The aim of the current study was to identify the essential oils of Juniperus oxycedrus from North Western Algeria (Mostaganem) by gas chromatography coupled to mass spectrometer (GC-MS), and to evaluate their antibac-terial and antioxidant activities. Extraction was carried out by two processes, hydrodistillation (HD) and steam distillation (SD). Analysis by GC-MS led to the identification of 38 components using both methods. HD was found to be the best process for the extraction of Juniperus essential oil, and the ma-jor identified compounds were: germacrene-D (38.28%), followed by α.-pinene (17.66%), and δ-cadinene (5.91%). In the antibacterial test, SD oil had a higher inhibitory effect. The strain Staphylococcus aureus was the most sensitive with an inhibition zone of 15.25 mm and a minimum inhibitory concentration (MIC) of 0.25 mg/mL. The antioxidant activity of J. oxycedrus es-sential oils obtained by HD was more important than SD (IC50= 19.52 mg/mL and IC50= 38.62 mg/mL) with DPPH and β-carotene bleaching tests respec-tively. Essential oils of J. oxycedrus could therefore represent good candidates to be used as antioxidant and antibacterial agents.
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KLANČNIK, ANJA, BERNARDA GUZEJ, MAJDA HADOLIN KOLAR, HELENA ABRAMOVIČ, and SONJA SMOLE MOŽINA. "In Vitro Antimicrobial and Antioxidant Activity of Commercial Rosemary Extract Formulations." Journal of Food Protection 72, no. 8 (August 1, 2009): 1744–52. http://dx.doi.org/10.4315/0362-028x-72.8.1744.
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Phenolic plant extracts are sources of natural bioactive compounds, which can inhibit the rate of food spoilage. MIC and MBC concentrations of four oil- or water-soluble rosemary (Rosmarinus officinalis) extracts against gram-positive (Bacillus and Staphylococcus) and gram-negative (Campylobacter and Salmonella) bacteria were determined by using disk diffusion, agar dilution, and broth microdilution methods, as well as bacterial survival kinetics in a macrodilution test. To describe the antioxidant properties of the extracts, the reducing power, free radical scavenging effectiveness, and β-carotene bleaching test were used. The antimicrobial and antioxidant activity depended on the concentration and chemical nature of the phenolic compounds in the extracts. Gram-positive bacteria were more sensitive than were gram-negative bacteria, especially for oil-soluble extracts with carnosic acid as the major phenolic compound. A microdilution method based on ATP measurement was found to be a useful, rapid technique for determining antibacterial efficiency, and its results correlated well with MICs from survival curve measurement. Reducing power and free radical scavenging effectiveness was higher in water-soluble formulations, according to their higher total phenolic content, but in an aqueous emulsion system of linoleic acid, they exhibited lower antioxidant activity. This correlated well with the higher efficiency of antimicrobial activity of oil-soluble formulations, despite the lower total phenolic content of these extracts.
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Leporini, Mariarosaria, Monica Rosa Loizzo, Rosa Tundis, Chiara La Torre, Alessia Fazio, and Pierluigi Plastina. "Non-Pungent n-3 Polyunsaturated Fatty Acid (PUFA)-Derived Capsaicin Analogues as Potential Functional Ingredients with Antioxidant and Carbohydrate-Hydrolysing Enzyme Inhibitory Activities." Antioxidants 8, no. 6 (June 5, 2019): 162. http://dx.doi.org/10.3390/antiox8060162.
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N-Eicosapentaenoyl vanillylamine (EPVA) and N-docosahexaenoyl vanillylamine (DHVA), derived from n-3 polyunsaturated eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), respectively, were studied for their potential antioxidant and carbohydrate-hydrolysing enzyme inhibitory activities together with capsaicin and the corresponding n-3 polyunsaturated fatty acids (PUFAs). The antioxidant potential was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay, β-carotene bleaching test, and ferric reducing ability power (FRAP). In the ABTS assay the following trend of potency could be observed EPVA > DHVA ≥ capsaicin. In addition, except for the FRAP test, all samples showed a greater activity than the positive controls used as reference compounds in the antioxidant assays. Both EPVA and DHVA showed half maximal inhibitory concentration (IC50) values much lower than acarbose, which was used as the reference drug in the carbohydrate-hydrolysing enzyme inhibitory activity assays. It is interesting to note that structural changes in capsaicin derivatives had higher impacts on α-glucosidase than on α-amylase inhibition. Taken together, our data suggest that both EPVA and DHVA, which are not limited in compliance-related considerations with respect to capsaicin, due to absence of pungency, could be proposed as functional ingredients for the development of products for the management of type II diabetes and border-line hyperglycaemic patients.
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Danert, Fátima C., Catiana Zampini, Roxana Ordoñez, Luis Maldonado, Enrique Bedascarrasbure, and María Inés Isla. "Nutritional and Functional Properties of Aqueous and Hydroalcoholic Extracts from Argentinean Propolis." Natural Product Communications 9, no. 2 (February 2014): 1934578X1400900. http://dx.doi.org/10.1177/1934578x1400900209.
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Bee propolis is a natural product extensively used as an ingredient in functional foods in amounts that may confer health benefits. The aim of this study was to investigate the nutritional and functional properties (antioxidant activity) of aqueous and ethanolic extracts of propolis samples from Santiago del Estero province, northern Argentina. All propolis extracts contained macronutrients (glucose, fructose, sucrose and proteins), micronutrients (Na, K, Ca, P and Mg) and trace elements (Fe). Spectrophotometric, TLC and HPLC-DAD analyses showed the presence of several phenolic, flavonoid and non-flavonoid compounds, but in all cases the flavonoids prevailed. The PCA of polyphenolic content provided a clear separation of propolis in Group 1 (SE1, 2, 3, 4 and 7) and Group 2 (SE5 and 6) associated with phenolic compound content and collection regions. Two compounds, pinocembrin and chrysin, which could be used as chemical markers of Santiago del Estero propolis, were identified in all samples. Propolis samples extracted with water presented better radical scavenging ability than ethanolic extracts, independent of the antioxidant method (scavenging activity of ABTS•+, DPPH•, HO• and O2•- and β-carotene bleaching test). Such results correlated closely with the levels of total phenols and flavonoids in samples. The results justify the use of Argentine propolis as a functional dietary supplement.
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Aćimović, Milica, Jovana Ljujić, Jelena Vulić, Valtcho D. Zheljazkov, Lato Pezo, Ana Varga, and Vesna Tumbas Šaponjac. "Helichrysum italicum (Roth) G. Don Essential Oil from Serbia: Chemical Composition, Classification and Biological Activity—May It Be a Suitable New Crop for Serbia?" Agronomy 11, no. 7 (June 24, 2021): 1282. http://dx.doi.org/10.3390/agronomy11071282.
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H. italicum essential oil (EO) is one of the most popular ingredients utilized by the cosmetic industry, and it is also used as natural antioxidant and as a value-added ingredient in food products. The chemical composition of the EO H. italicum cultivated in Serbia was analyzed by gas chromatography–mass spectrometry. The quantitative structure–retention relationship was used to predict the retention indices of the EO constituents acquired by GC-MS data, applying five molecular descriptors selected by factor analysis and a genetic algorithm. Also, antimicrobial activity, and biological activity by four common antioxidant tests (DPPH and ABTS assays, reducing power, and β-carotene bleaching test), and in vitro antihyperglycemic and anti-inflammatory capacities were evaluated. A total of 70 EO constituents were detected, of which 17 (8.5%) could not be identified. The H. italicum EO in this study belonged to γ-curcumene chemotype. The coefficients of determination reached the value of 0.964, demonstrating that this model could be used for prediction purposes. All applied tests showed that H. italicum EO possesses good biological activity and an interesting chemical composition. Therefore, the EO of H. italicum grown in Serbia has a potential to be used in food, cosmetic, and pharmaceutical products.
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Loizzo, Monica Rosa, Marco Bonesi, Mariarosaria Leporini, Tiziana Falco, Vincenzo Sicari, and Rosa Tundis. "Chemical Profile and In Vitro Bioactivity of Vicia faba Beans and Pods." Proceedings 70, no. 1 (November 10, 2020): 45. http://dx.doi.org/10.3390/foods_2020-07712.
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Vicia faba L. (faba bean) (Fabaceae) is cultivated worldwide as a crop for human consumption. In this study, beans and pods were investigated for their phytochemical content and their potential nutraceutical properties as a strategy to counteract metabolic syndrome (MetS). Pods represent a faba bean industrial processing by-product. Pod ethanol extract showed the highest total phenol and flavonoid content. High Performance Liquid Chromatography (HPLC) analysis revealed that in both pods and bean, (+)-catechin and (−)-epicatechin were the two most abundant compounds. 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS), Ferric Reducing Antioxidant Power (FRAP), β-carotene bleaching were used to test V. faba antioxidant activity. The inhibition of alpha-amylase, alpha-glucosidase, and lipase was studied. Pod extract showed an ABTS radical scavenging ability (IC50 value of 1.5 mg/mL) comparable to ascorbic acid (IC50 value of 1.7 mg/mL) used as a positive control, whereas bean extract was the most active in protecting lipid peroxidation. A promising alpha-glucosidase inhibitory activity was also observed with the edible portion of faba beans (IC50 value of 38.31 mg/mL). Collectively, our results demonstrated the potential health properties of V. faba edible and inedible portions.
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Brodkiewicz, Yanina, Karenina Marcinkevicius, Marcos Reynoso, Virginia Salomon, Luis Maldonado, and Nancy Vera. "STUDIES OF THE BIOLOGICAL AND THERAPEUTIC EFFECTS OF ARGENTINE STINGLESS BEE PROPOLIS." Journal of Drug Delivery and Therapeutics 8, no. 5 (September 11, 2018): 382–92. http://dx.doi.org/10.22270/jddt.v8i5.1889.
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Meliponids are native bees of the Americas, characterized by having no sting (ANSA). Some live in the Yunga forests of northern Argentina, a place rich in a diversity of this type of bees of which at least thirty-three species may be found. Propolis is a resinous substance that bees collect from plants exudates. Chemical composition and functional properties vary according to the flora of the place where the hives are. They have been valued by humans for their biological properties for centuries. This study is aimed at investigating the antinociceptive, antioxidant and anti-biofilm activities of propolis from the stingless bees Tetragonisca fiebrigi Schwarz and Scaptotrigona jujuyensis Schrottky found in Estación Experimental Agropecuaria Famaillá of INTA, Tucumán, Argentina. Analgesic activity of the extracts was estimated against acetic acid induced writhing, tail immersion method and formalin test. Antioxidant capacity was evaluated using DPPH free radical scavenging and β-carotene bleaching assays. Propolis anti biofilm activity was tested on Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATTC 6538P bacteria. Phytochemical constituents were obtained using standard chemical methods. The propolis alcoholic extracts of the studied species possess antinociceptive activity at both central and peripheral levels as demonstrated by the three algesia tests used. Both propolis extracts were effective antioxidants in DPPH and β-carotene linoleic acid model systems. S. jujuyensis propolis tested at all doses against S. aureus and P. aeruginosa presented a selective biofilm inhibition unrelated to bacterial growth inhibition, thus achieving a reduction in pathogenicity. The chemical studies revealed the presence of sterols, triterpenes, catechins, coumarins, flavonoids, phenols, tannins and anthocyanidins. Chemical composition observed in the T.fiebrigi and S. jujuyensis propolis, suggest that those responsible for the activity would be chemical compounds of a non-phenolic nature. Our data indicate that geopropolis is a natural source of bioactive substances with promising beneficial properties for human health. Isolation and identification of compounds responsible for the pharmacological activities displayed by propolis has started.
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Tundis, Rosa, Carmela Conidi, Monica R. Loizzo, Vincenzo Sicari, and Alfredo Cassano. "Olive Mill Wastewater Polyphenol-Enriched Fractions by Integrated Membrane Process: A Promising Source of Antioxidant, Hypolipidemic and Hypoglycaemic Compounds." Antioxidants 9, no. 7 (July 10, 2020): 602. http://dx.doi.org/10.3390/antiox9070602.
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The valorisation of food wastes is a challenging opportunity for the green, sustainable, and competitive development of industry. The recovery of phenols contributes to the sustainability of olive waste sector, reducing its environmental impact and promoting the development of innovative formulations of interest for pharmaceutical, nutraceutical, and cosmeceutical applications. In this work, olive mill wastewater was treated through a combination of microfiltration (MF), nanofiltration (NF), and reverse osmosis (RO) in a sequential design to produce polyphenol-enriched fractions that have been investigated for their chemical profile using ultra-high-performance liquid chromatography (UHPLC), and their potential antioxidant, hypolipidemic, and hypoglycaemic activities. RO retentate exhibited the highest content of hydroxytyrosol, tyrosol, oleuropein, verbascoside, vanillic acid, and luteolin. In particular, a content of hydroxytyrosol of 1522.2 mg/L, about five times higher than the MF feed, was found. RO retentate was the most active extract in all in vitro tests. Interestingly, this fraction showed a 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS) radicals scavenging activity with an IC50 value of 6.9 μg/mL and a potential inhibition of lipid peroxidation evaluated by the β-carotene bleaching test with IC50 values of 25.1 μg/mL after 30 min of incubation. Moreover, RO retentate inhibited α-amylase and α-glucosidase with IC50 values of 65.3 and 66.2 μg/mL, respectively.
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Gonçalves, Joana, Ângelo Luís, Ana Gradillas, Antonia García, José Restolho, Nicolás Fernández, Fernanda Domingues, Eugenia Gallardo, and Ana Paula Duarte. "Ayahuasca Beverages: Phytochemical Analysis and Biological Properties." Antibiotics 9, no. 11 (October 24, 2020): 731. http://dx.doi.org/10.3390/antibiotics9110731.
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Ayahuasca is a psychoactive beverage, originally consumed by indigenous Amazon tribes, of which consumption has been increasing worldwide. The aim of this study was to evaluate the phytochemical profile, as well as the antioxidant, anti-inflammatory and antimicrobial properties of decoctions of four individual plants, a commercial mixture and four mixtures of two individual plants used in the Ayahuasca preparation. For this purpose, a phytochemical characterization was performed, determining the content of flavonoids, total phenolic compounds, and analyzing the phenolic profile. Besides, 48 secondary metabolites were investigated by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q/TOF-MS) and their concentration estimated with real standards when present. The antioxidant activity was evaluated by both the β-carotene bleaching test and DPPH free radical scavenging assay, and the anti-inflammatory activity was determined by a protein denaturation method. Finally, the antimicrobial properties were evaluated using the disc diffusion assay, resazurin microtiter method, anti-quorum sensing and anti-biofilm activity assays. The obtained results showed that, in general, the samples have a high content of phenolic compounds and flavonoids with noticeable differences, reflecting on remarkable antioxidant and anti-inflammatory activities. Significant antimicrobial properties were also observed, with emphasis on the effect of B. caapi and P. harmala on planktonic and biofilm cells of A. baumannii, inhibiting both the biofilm formation and the production of violacein pigment.
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Tundis, Rosa, Carmela Conidi, Monica R. Loizzo, Vincenzo Sicari, Rosa Romeo, and Alfredo Cassano. "Concentration of Bioactive Phenolic Compounds in Olive Mill Wastewater by Direct Contact Membrane Distillation." Molecules 26, no. 6 (March 23, 2021): 1808. http://dx.doi.org/10.3390/molecules26061808.
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Olive mill wastewater (OMW), generated as a by-product of olive oil production, is considered one of the most polluting effluents produced by the agro-food industry, due to its high concentration of organic matter and nutrients. However, OMW is rich in several polyphenols, representing compounds with remarkable biological properties. This study aimed to analyze the chemical profile as well as the antioxidant and anti-obesity properties of concentrated fractions obtained from microfiltered OMW treated by direct contact membrane distillation (DCMD). Ultra-high performance liquid chromatography (UHPLC) analyses were applied to quantify some phenols selected as phytochemical markers. Moreover, α-Amylase, α-glucosidase, and lipase inhibitory activity were investigated together with the antioxidant activity by means of assays, namely β-carotene bleaching, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic) acid (ABTS) diammonium salts, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, and Ferric Reducing Activity Power (FRAP) tests. MD retentate—which has content of about five times greater of hydroxytyrosol and verbascoside and about 7 times greater of oleuropein than the feed—was more active as an antioxidant in all applied assays. Of interest is the result obtained in the DPPH test (an inhibitory concentration 50% (IC50) of 9.8 μg/mL in comparison to the feed (IC50 of 97.2 μg/mL)) and in the ABTS assay (an IC50 of 0.4 μg/mL in comparison to the feed (IC50 of 1.2 μg/mL)).
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Telichowska, Aleksandra, Joanna Kobus-Cisowska, Piotr Szulc, Marta Ligaj, Kinga Stuper-Szablewska, Dominik Szwajgier, and Henryk Bujak. "Comparative Analysis of Infusions with the Addition P. padus Bark: Assessment of the Antioxidant Potential and Their Inhibitory Effect on Enzymes Associated with Oxidative Stress." Sustainability 13, no. 7 (April 1, 2021): 3913. http://dx.doi.org/10.3390/su13073913.
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Prunus padus L., is not very popular plant, it is commonly found due to low soil requirements and easy to settle in various places. As for now, concerning food technology, there is no wide application for P. padus. Therefore, the aim of this study is to evaluate the possibility of using bird cherry bark as an ingredient in herbal functional teas. In the first step, the conditions for extraction of the bark were electrochemically optimized. It was proven that the highest content of polyphenols could be found in the sample consisting of chamomile, linden flower, and calendula (7939.8 ± 106.6 mg/100 g dm). In the beta-carotene bleaching test, the highest activity could be spotted for calendula tea (16.7 ± 1.1c%) and chamomile tea (15.0 ± 2.0c%) and concerning the test for linden flower tea without added bark (134.4 ± 15.1b μg ascorbic acid /mL). The property of the tested teas to inhibit cholinesterases was proven. What is more, P. padus bark infusion showed the highest activity of 15.8 ± 1.1d μg neostigmine/mL, for acetylcholinesterases (AChE) inhibition and 21.2 ± 1.0c μg neostigmine/mL for butyrylcholinesterases (BChE). The same tea also showed the highest activity to reduce ions of iron (Fe(III)): 25.3 ± 0.9c μg Trolox /mL and glutathione reductase and glutathione peroxidase inhibition, 87.0 ± 1.1e% and 64.9 ± 2.0d% respectively. The use of P. padus bark may be vital in the preventive care concerning modern-age diseases and allow for the production of a new range of products with distinctive sensory characteristics and functional properties and, at the same time, in combating the spread of P. padus in the farm and forest ecosystem.
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Tundis, Rosa, Mariarosaria Leporini, Marco Bonesi, Simone Rovito, and Nicodemo G. Passalacqua. "Salvia officinalis L. from Italy: A Comparative Chemical and Biological Study of Its Essential Oil in the Mediterranean Context." Molecules 25, no. 24 (December 10, 2020): 5826. http://dx.doi.org/10.3390/molecules25245826.
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Salvia officinalis L. (sage) is one of the most appreciated plants for its plethora of biologically active compounds. The objective of our research was a comparative study, in the Mediterranean context, of chemical composition, anticholinesterases, and antioxidant properties of essential oils (EOs) from sage collected in three areas (S1–S3) of Southern Italy. EOs were extracted by hydrodistillation and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory properties were investigated by employing Ellman’s method. Four in vitro assays, namely, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ferric-reducing ability power (FRAP), and β-carotene bleaching tests, were used to study the antioxidant effects. Camphor (16.16–18.92%), 1,8-cineole (8.80–9.86%), β-pinene (3.08–9.14%), camphene (6.27–8.08%), and α-thujone (1.17–9.26%) are identified as the most abundant constituents. However, the content of these constituents varied depending on environmental factors and pedoclimatic conditions. Principal component analysis (PCA) was performed. Based on Relative Antioxidant Capacity Index (RACI), S2 essential oil exhibited the highest radical potential with an IC50 value of 20.64 μg/mL in ABTS test and presented the highest protection of lipid peroxidation with IC50 values of 38.06 and 46.32 μg/mL after 30 and 60 min of incubation, respectively. The most promising inhibitory activity against BChE was found for S3 sample (IC50 of 33.13 μg/mL).
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Amodeo, Valentina, Mariangela Marrelli, Veronica Pontieri, Roberta Cassano, Sonia Trombino, Filomena Conforti, and Giancarlo Statti. "Chenopodium album L. and Sisymbrium officinale (L.) Scop.: Phytochemical Content and In Vitro Antioxidant and Anti-Inflammatory Potential." Plants 8, no. 11 (November 15, 2019): 505. http://dx.doi.org/10.3390/plants8110505.
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Spontaneous edible plants have an old history of use in popular traditions all around the world, and the rediscovery of these species could also be useful for the search of new drugs. Chenopodium album L. (Amaranthaceae) and Sisymbrium officinale (L.) Scop. (Brassicaceae) are two annual plants traditionally used both as food and herbal remedies against inflammatory disorders. In this work, the potential anti-inflammatory and anti-arthritic activities of these plant species have been investigated, together with their antioxidant potential. The phytochemical composition was assessed as well by means of gas chromatography coupled to mass spectrometry (GC-MS) and high performance thin layer chromatography (HPTLC). The antioxidant properties were assessed using the DPPH and β-carotene bleaching test. The ability of extracts to protect against lipid peroxidation was also examined in rat-liver microsomal membranes. All the samples showed a preservation of antioxidant activity up to 60 min. A significant inhibitory activity on the production of the pro-inflammatory mediator nitric oxide was induced in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells by the dichloromethane fraction of C. album extract, with an IC50 value equal to 81.7 ± 0.9 μg/mL. The same sample showed also a concentration-dependent anti-denaturation effect on heat-treated bovine serum albumin (IC50 = 975.6 ± 5.5 μg/mL), even if the best in vitro anti-arthritic activity was observed for the dichloromethane fraction of S. officinale extract, with an IC50 value of 680.9 ± 13.2 μg/mL.
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Conforti, F., M. C. Marcotullio, F. Menichini, G. A. Statti, L. Vannutelli, G. Burini, F. Menichini, and M. Curini. "The Influence of Collection Zone on Glucosinolates, Polyphenols and Flavonoids Contents and Biological Profiles of Capparis sicula ssp. sicula." Food Science and Technology International 17, no. 2 (March 24, 2011): 87–97. http://dx.doi.org/10.1177/1082013210368583.
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This study aimed to evaluate the influence of collection zone on total phenol, flavonoid and glucosinolate contents and antioxidant and anti-inflammatory activities of caper ( Capparis sicula ssp. sicula). This species has been characterized through the detection, isolation and quantitative evaluation of chemical markers (polyphenols, flavonoids and glucosinolates). The chemical investigation showed a different composition between the two collection zones. While the total amounts of phenolics and flavonoids of the two samples were quite the same, their high-performance liquid chromatography profiles were very different. In both samples, the most abundant aglycone was quercetin which accounted for 60% of total flavonoids. Nuclear magnetic resonance data analysis allowed the identification of two compounds: 3,5-dicaffeoylquinic and 4,5-dicaffeoylquinic acids which represented 6.67% and 15.94%, respectively, of the total amount of flavonoids in sample 1. In sample 2, these two acids were still present, but their percentages were much less (2.20% and 1.71%, respectively). As far as we know, this is the first report about the presence of dicaffeoylquinic acids in Capparis. With regard to glucosinolate content, sample 1 showed a higher content of glucosinolates. In both samples, glucocapparin was the most abundant compound. Antioxidant activity of the methanolic C. sicula extracts using diphenyl picrylhydrazyl, β-carotene bleaching test and oxygen radical absorbance capacity showed that the sample 2 was more active than 1. As regards the inhibition of NO production, the extracts from sample 2 were more active than those from sample 1.