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Toae, Sriroth, Rojanaridpiched, Vichukit, Chotineeranat, Wansuksri, Chatakanonda, and Piyachomkwan. "Outstanding Characteristics of Thai Non-GM Bred Waxy Cassava Starches Compared with Normal Cassava Starch, Waxy Cereal Starches and Stabilized Cassava Starches." Plants 8, no. 11 (October 24, 2019): 447. http://dx.doi.org/10.3390/plants8110447.
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Waxy cassava roots of nine varieties successfully developed in Thailand by a non-genetic modification (non-GM), conventional breeding method were used for extracting starches and their starch physico-chemical properties were evaluated and compared with normal cassava starches, commercial waxy starches (i.e., waxy maize starch and waxy rice starch) and commercial stabilized starches (i.e., acetylated starch and hydroxypropylated starch). All waxy cassava varieties provided starches without amylose while normal cassava starches contained 18%–20% amylose contents. As determined by a Rapid Visco Analyzer (RVA) at 5% (dry basis), waxy cassava starches had the highest peak viscosity and the lowest setback viscosity. Cooked paste of waxy cassava starches had the greatest clarity and stability among all starches during storage at 4 ℃ for 7 days as evidenced by its high light transmittance (%T) at 650 nm. No syneresis was detected in waxy cassava starch gels after subjecting to four freeze-thaw cycles (4 weeks) indicating high potential use of waxy cassava starches, free from chemicals, to replace stabilized starches as thickening and texturing agents in food products.
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Ayetigbo, Oluwatoyin, Sajid Latif, Adebayo Abass, and Joachim Müller. "Comparing Characteristics of Root, Flour and Starch of Biofortified Yellow-Flesh and White-Flesh Cassava Variants, and Sustainability Considerations: A Review." Sustainability 10, no. 9 (August 30, 2018): 3089. http://dx.doi.org/10.3390/su10093089.
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Cassava is a significant food security and industrial crop, contributing as food, feed and industrial biomass in Africa, Asia and South America. Breeding efforts have led to the development of cassava variants having desirable traits such as increased root, flour, and starch yield, reduced toxicity, reduced pest/disease susceptibility and improved nutrient contents. Prominent among those breeding efforts is the development of colored-flesh cassava variants, especially biofortified yellow-fleshed ones, with increased pro-vitamin A carotenoids, compared to the white-flesh variants. The concept of sustainability in adoption of biofortified yellow-flesh cassava and its products cannot be fully grasped without some detailed information on its properties and how these variants compare to those of the white-flesh cassava. Flour and starch are highly profitable food products derived from cassava. Cassava roots can be visually distinguished based on flesh color and other physical properties, just as their flours and starches can be differentiated by their macro- and micro-properties. The few subtle differences that exist between cassava variants are identified and exploited by consumers and industry. Although white-flesh variants are still widely cultivated, value addition offered by biofortified yellow-flesh variants may strengthen acceptance and widespread cultivation among farmers, and, possibly, cultivation of biofortified yellow-flesh variants may outpace that of white-flesh variants in the future. This review compares properties of cassava root, flour, and starch from white-flesh and biofortified yellow-flesh variants. It also states the factors affecting the chemical, functional, and physicochemical properties; relationships between the physicochemical and functional properties; effects of processing on the nutritional properties; and practical considerations for sustaining adoption of the biofortified yellow-flesh cassava.
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LESTARI, TRI, and RION APRIYADI. "Genetic potential of cassava biodiversity in Bangka Island, Indonesia." Cell Biology and Development 1, no. 2 (December 1, 2017): 41–45. http://dx.doi.org/10.13057/cellbioldev/v010201.
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Lestari T, Apriyadi R. 2017. Genetic potential of cassava biodiversity in Bangka Island, Indonesia. Cell Biol Dev 2: 41-45. Cassava is potential as a mixture ingredient of flour in the Bangka’s food industry. This study aimed to discover the biodiversity of local cassava in Bangka. This research was conducted in experimental field of the Faculty of Agriculture, University of Bangka Belitung, Indonesia from July 2015 to July 2016. The experimental design was randomized block design with 10 local cassavas of Bangka that consisted of upang, sekula, bayel, mentega, kuning, batin, pulut, sutera, rakit, and Selangor. Isozyme analysis performed using starch gel electrophoresis with horizontal models. Analysis for five Bangka local cassava varieties and one National cassava variety used RAPD group OP A and OP B. The results showed that the phenotypic performance was different on the type of plant, the morphology of leaves, stems, and tubers of local cassava of Bangka. Isozyme analysis showed polymorphic banding pattern, while the eight RAPD primers used did not produce polymorphic. This research showed Bangka local cassava morphologically different based on visual observation. Morphological character of Bangka local cassava leaf was divided into three shapes of lobe: ellipse (upang, sekula, bayel, mentega, batin, pulut, rakit, Selangor), linear (kuning) and lanceolate (sutera). This research data showed that the genetic diversity of local cassava in Bangka relatively high. Bangka local cassava has genetic potential as plant propagation material for plant breeding.
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Subekti, Isnani, Nurul Khumaida, Sintho Wahyuning Ardie, and Dan Muhamad Syukur. "Evaluasi Hasil dan Kandungan Pati Mutan Ubi Kayu Hasil Iradiasi Sinar Gamma Generasi M1V4." Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) 46, no. 1 (April 27, 2018): 64. http://dx.doi.org/10.24831/jai.v46i1.17610.
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<em>Cassava is one of the important starch producer crops. Although Indonesia ranks as the third largest cassava producer country, Indonesia still imports cassava starch to meet the domestic demand in food and non-food industries. Gamma ray irradiation has been implemented to generate cassava mutants in order to support development of superior cassava variety, especially high yield and high starch content, especially high yield and high starch content. The objectives of this research were to evaluate the yield potential and the starch content of gamma irradiated 40 putative cassava mutants at M1V4 generation. This research was arranged in randomized complete block design with three replications and conducted at Cikabayan Experimental Field, IPB, Bogor, from May 2016 to March 2017. The starch content was measured using gravimetric methods. The results showed that five mutants, G2142, G3151, G2141, G1143, and G3111, had higher tuber weight per plant (6-7 kg per plant) compared to the background ‘Gajah’ genotype (4.7 kg per plant). ‘G2112’ mutant genotype showed higher starch content (23.86%) compared to the background ‘Gajah’ genotype (17.65%). The specific gravity (SG) was positively correlated with starch content (r = 0.905), indicated that the higher the SG, the higher the starch content of cassava.<br /><br />Keywords: gravimetric, mutant, specific gravity (SG), yield</em>
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Rossitto De Marchi, Bruno, Tonny Kinene, Renate Krause-Sakate, Laura M. Boykin, Joseph Ndunguru, Monica Kehoe, Elijah Ateka, Fred Tairo, Jamisse Amisse, and Peter Sseruwagi. "Genetic diversity and SNP’s from the chloroplast coding regions of virus-infected cassava." PeerJ 8 (March 2, 2020): e8632. http://dx.doi.org/10.7717/peerj.8632.
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Cassava is a staple food crop in sub-Saharan Africa; it is a rich source of carbohydrates and proteins which currently supports livelihoods of more than 800 million people worldwide. However, its continued production is at stake due to vector-transmitted diseases such as Cassava mosaic disease and Cassava brown streak disease. Currently, the management and control of viral diseases in cassava relies mainly on virus-resistant cultivars of cassava. Thus, the discovery of new target genes for plant virus resistance is essential for the development of more cassava varieties by conventional breeding or genetic engineering. The chloroplast is a common target for plant viruses propagation and is also a potential source for discovering new resistant genes for plant breeding. Non-infected and infected cassava leaf samples were obtained from different locations of East Africa in Tanzania, Kenya and Mozambique. RNA extraction followed by cDNA library preparation and Illumina sequencing was performed. Assembling and mapping of the reads were carried out and 33 partial chloroplast genomes were obtained. Bayesian phylogenetic analysis from 55 chloroplast protein-coding genes of a dataset with 39 taxa was performed and the single nucleotide polymorphisms for the chloroplast dataset were identified. Phylogenetic analysis revealed considerable genetic diversity present in chloroplast partial genome among cultivated cassava of East Africa. The results obtained may supplement data of previously selected resistant materials and aid breeding programs to find diversity and achieve resistance for new cassava varieties.
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Sukara, E., and H. W. Doelle. "Cassava starch fermentation pattern ofRhizopus oligosporus." MIRCEN Journal of Applied Microbiology and Biotechnology 4, no. 4 (1988): 463–71. http://dx.doi.org/10.1007/bf00940173.
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Olayide, Priscilla, Annabel Large, Linnea Stridh, Ismail Rabbi, Susanne Baldermann, Livia Stavolone, and Erik Alexandersson. "Gene Expression and Metabolite Profiling of Thirteen Nigerian Cassava Landraces to Elucidate Starch and Carotenoid Composition." Agronomy 10, no. 3 (March 20, 2020): 424. http://dx.doi.org/10.3390/agronomy10030424.
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The prevalence of vitamin A deficiency in sub-Saharan Africa necessitates effective approaches to improve provitamin A content of major staple crops. Cassava holds much promise for food security in sub-Saharan Africa, but a negative correlation between β-carotene, a provitamin A carotenoid, and dry matter content has been reported, which poses a challenge to cassava biofortification by conventional breeding. To identify suitable material for genetic transformation in tissue culture with the overall aim to increase β-carotene and maintain starch content as well as better understand carotenoid composition, root and leaf tissues from thirteen field-grown cassava landraces were analyzed for agronomic traits, carotenoid, chlorophyll, and starch content. The expression of five genes related to carotenoid biosynthesis were determined in selected landraces. Analysis revealed a weak negative correlation between starch and β-carotene content, whereas there was a strong positive correlation between root yield and many carotenoids including β-carotene. Carotenoid synthesis genes were expressed in both white and yellow cassava roots, but phytoene synthase 2 (PSY2), lycopene-ε-cyclase (LCYε), and β-carotenoid hydroxylase (CHYβ) expression were generally higher in yellow roots. This study identified lines with reasonably high content of starch and β-carotene that could be candidates for biofortification by further breeding or plant biotechnological means.
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Oyadiran, TF, and JA Osakwe. "Effect of African cassava mosaic disease on morphological characteristics of cassava, Manihot esculenta (Crantz)." Journal of Agriculture, Forestry and the Social Sciences 11, no. 2 (February 18, 2015): 258–65. http://dx.doi.org/10.4314/joafss.v11i2.30.
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Field trials were conducted with 10 improved clones of cassava to study the effect of ACMD on their morphological characteristics. The clones used were: 97 / 4763, 97/2205, 91/02324, 98/0505, M98/0068, 96/1089A, 96/1632, M98/0040, 99/2123 and 97/0162. The parameters measured were plant height, leaf area, number of branches and petiole length. The research revealed differences within the clones for the parameters studied, these differences seemed to be mainly genetic, as they were not positively correlated to the disease incidence and severity. Based on the findings in this research the following recommendations were made. On the basis of their branching pattern, highly branched clones 97 / 2205, 97/4763 and 98 /0505 are recommended for selection. The heavy branches bear a lot of leaves or produce a thick canopy which helps to reduce the need for weeding, and also reduce the effect of loss of leaves that occur due to disease incidence. However, for industrial / mechanized agriculture, clones that are less branched should be selected as they are easier to harvest mechanically. Though there is disadvantage with their small leaf areas; the crops would have to be highly resistant to fungal attack which tends to cause leaf drop that will reduce leaf material for food / starch production (examples of less branching clones are, M98 / 0068, 96/1632,91/02324).Key Word: African Cassava Mosaic Disease (ACMD), Cassava Mosaic Virus.(CMV)
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Njoku, Damian Ndubuisi, and Emmanuel Ukaobasi Mbah. "Assessment of yield components of some cassava (Manihot esculenta Crantz) genotypes using multivariate analysis such as path coefficients." Open Agriculture 5, no. 1 (September 8, 2020): 516–28. http://dx.doi.org/10.1515/opag-2020-0051.
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AbstractCassava, which is a tropical storage root crop, serves as a veritable source of carbohydrate for people living in Sub-Saharan Africa. It is also an important source of industrial starch, biopolymers, animal feeds and ethanol. However, the genetic improvement of the crop is impeded by dearth in information on its genetic variability. A field experiment was carried out at the National Root Crops Research Institute, Umudike (05° 29′ N; 07° 33′ E; 122 m a.s.l.), Nigeria, aimed at assessing the interrelationship between fresh root yield and thirteen quantitative yield-related characters of twenty-eight (28) newly developed cassava genotypes. The rain-fed experiment was laid out in a randomized complete block design with dual replications in 2016/2017 cropping seasons. The results indicated that the newly developed cassava genotypes [2] NR110315 followed by [20] B1-5 and [1] NR110238 exhibited (P < 0.05) highest fresh root yield among the twenty-eight genotypes tested. The fresh root yield of [2] NR110315 (18.77 Mt ha−1) was relatively higher compared with [18] NR050080, which gave the least root yield of 5.38 Mt ha−1. The correlation coefficients obtained from the associations among the variables were positive and exhibited highly significant (P ≤ 0.01) association, especially between leaf area index (LAI) and root diameter as well as with fresh root yield of cassava. Among the nine variables subjected to the path analysis, LAI, starch content, above ground dry matter (AGDM), weight of fresh marketable roots per plant and root length that contributed positively and directly to higher fresh root yield require greater attention during selection and breeding in cassava studies. The direct effect of the variables indicating their magnitudes in decreasing order showed that LAI exhibited the greatest effect followed by starch content, AGDM, weight of fresh marketable roots plant−1, root length, % cassava bacterial blight incidence and % Cassava mosaic disease incidence, which had the lowest direct effect on root yield of cassava. Cyanide potential exhibited a direct, weak and negative effect on fresh root yield. The results further suggested that cassava improvement could be achieved through these characters that have positive and highly significant magnitude effect on fresh root yield.
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Khumaida, Nurul, Sintho Wahyuning Ardie, and Mira Sri Astuti. "Characterization of Irradiation Induced Mutants of Cassava (Manihot esculenta Crantz) Generated from Jame-jame and Adira-4 Genotypes at M1V2 Generation." KnE Life Sciences 2, no. 6 (November 26, 2017): 22. http://dx.doi.org/10.18502/kls.v2i6.1016.
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Cassava (Manihot esculenta Crantz., Euphorbiaceae) is an important dietary carbohydrate source for approximately 800 million people in the tropics. It is a potential crop for food, feed, and industry. Cassava breeding through conventional approaches are hampered with some limitations which resulted in a low number of superior varieties. The objective of this research was to generate several mutant lines with higher yield and starch content. This research intended to identify the second generation (M1V2) Jame-jame and Adira-4 variant cassava mutants from gamma ray irradiation. Jame-jame is a local genotype from Halmahera (local high yielding genotype, sweet, and potential) and Adira-4 is an Indonesia national variety of cassava. Mutant diversity appears on the plant height, plant branching, and plant tubers that harvested at 10 months after planting (MAP). Our results showed the positive correlation between plant height, first branching height, tuber weight, number of roots, and number of economic roots. Based on tuber weight and number of economic tuber there were 9 potential mutants (putative). Based on the stability test, there were 6 potential stable mutants (putative) by first branching height, tuber weight, total number of tubers, and total number of economies tuber, namely: V5D1-(2), V5D1-2(2), V5D1-3(2), V5D1-4(3), V5D2-2(2), and V5D2-6(2). Keywords: local genotype, M1V2, storage root, characters, stability.
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Hyde, Peter T., Xian Guan, Viviane Abreu, and Tim L. Setter. "The anti-ethylene growth regulator silver thiosulfate (STS) increases flower production and longevity in cassava (Manihot esculenta Crantz)." Plant Growth Regulation 90, no. 3 (September 20, 2019): 441–53. http://dx.doi.org/10.1007/s10725-019-00542-x.
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Abstract Cassava, which produces edible starchy roots, is an important staple food for hundreds of millions of people in the tropics. Breeding of cassava is hampered by its poor flower production, flower abortion, and lack of reproductive prolificacy. The current work determined that ethylene signalling affects floral development in cassava and that the anti-ethylene plant growth regulator silver thiosulfate (STS) mitigates the effects of ethylene on flower development. STS did not affect the timing of flower initiation, but improved early inflorescence and flower development as well as flower longevity such that flower numbers were increased. STS did not affect shoot and storage root growth. Studies of silver accumulation and treatment localization support the hypothesis that the beneficial effects of STS are confined to tissues of the shoot apex. The most effective timing of application was before inflorescence appearance extending to post-flower appearance. Based on this work a recommended protocol for STS use was developed. This work has the potential to improve methods for enhancing cassava flower development in breeding nurseries and thereby synchronize flowering of desired parents and enable the production of abundant progeny of desired crosses.
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Adu, Bright Gyamfi, Richard Akromah, Stephen Amoah, Daniel Nyadanu, Alex Yeboah, Lawrence Missah Aboagye, Richard Adu Amoah, and Eva Gyamfuaa Owusu. "High-density DArT-based SilicoDArT and SNP markers for genetic diversity and population structure studies in cassava (Manihot esculenta Crantz)." PLOS ONE 16, no. 7 (July 27, 2021): e0255290. http://dx.doi.org/10.1371/journal.pone.0255290.
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Cassava (Manihot esculenta Crantz) is an important industrial and staple crop due to its high starch content, low input requirement, and resilience which makes it an ideal crop for sustainable agricultural systems and marginal lands in the tropics. However, the lack of genomic information on local genetic resources has impeded efficient conservation and improvement of the crop and the exploration of its full agronomic and breeding potential. This work was carried out to obtain information on population structure and extent of genetic variability among some local landraces conserved at the Plant Genetic Resources Research Institute, Ghana and exotic cassava accessions with Diversity Array Technology based SilicoDArT and SNP markers to infer how the relatedness in the genetic materials can be used to enhance germplasm curation and future breeding efforts. A total of 10521 SilicoDArT and 10808 SNP markers were used with varying polymorphic information content (PIC) values. The average PIC was 0.36 and 0.28 for the SilicoDArT and SNPs respectively. Population structure and average linkage hierarchical clustering based on SNPs revealed two distinct subpopulations and a large number of admixtures. Both DArT platforms identified 22 landraces as potential duplicates based on Gower’s genetic dissimilarity. The expected heterozygosity which defines the genetic variation within each subpopulation was 0.008 for subpop1 which were mainly landraces and 0.391 for subpop2 indicating the homogeneous and admixture nature of the two subpopulations. Further analysis upon removal of the duplicates increased the expected heterozygosity of subpop1 from 0.008 to 0.357. A mantel test indicated strong interdependence (r = 0.970; P < 0.001) between SilicoDArT and DArTSeq SNP genotypic data suggesting both marker platforms as a robust system for genomic studies in cassava. These findings provide important information for efficient ex-situ conservation of cassava, future heterosis breeding, and marker-assisted selection (MAS) to enhance cassava improvement.
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Santos, Thais Paes Rodrigues dos, Célia Maria Landi Franco, Martha Maria Mischan, Daiana de Souza Fernandes, Marília Sbragia Del Bem, and Magali Leonel. "Production of partially gelatinized cassava starch: effects of preheating temperature and starch concentration on physicochemical characteristics during the spray-drying process." Australian Journal of Crop Science, no. 13(09):2019 (September 20, 2019): 1486–94. http://dx.doi.org/10.21475/ajcs.19.13.09.p1568.
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Starch has application in several industrial sectors, such as food, textile, paper, pharmaceutical, among other industries. To meet this demand, native starches have been modified by chemical, physical and enzymatic methods. Cassava is the second source of starch. Furthermore, understanding the effects of spray-drying modification on the structural and physicochemical characteristics of its starch is important. Therefore, this study aimed to evaluate the effects of the main interfering factors in the process of spray-drying on the characteristics of cassava starch, aiming at increasing the industrial applicability of this starch. A Central Composite Rotational Design (CCRD) was employed to assess the experimental data. Experimental design included four factorial points, four axial points and three replicates of the central point. The starch concentration ranges from 5 to 11 % and preheating temperature ranges from 54 to 60 °C. Results showed influence of the variable factors on the characteristics of cassava starch. The processing conditions that allowed obtaining pregelatinized starches with higher viscosity at room temperature, higher resistance to hot and agitation, lower retrogradation tendency, and with partial solubility, desired quality parameters for this product were: 6 % of starch concentration (wet basis) and 60 ᵒC of preheating temperature.
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Garg, S. K., and H. W. Doelle. "Optimization of cassava starch conversion to glucose byRhizopus oligosporus." MIRCEN Journal of Applied Microbiology and Biotechnology 5, no. 3 (September 1989): 297–305. http://dx.doi.org/10.1007/bf01741759.
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Fitriani, Hani, N. Sri Hartati, and Enny Sudarmonowati. "Evaluation of Adaptation and Production of Three Selected Cassava (Manihot esculenta Crantz) in Peat Land Area of Central Kalimantan." Jurnal ILMU DASAR 20, no. 2 (July 16, 2019): 75. http://dx.doi.org/10.19184/jid.v20i2.7449.
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Along with the increasing the of the land-use change of the fertile agricultural land in Java, the better awareness has been subjected to the importance of function peat land for agricultural development. Cassava (Manihot esculenta Crantz) is a tuber plant that potentially developed as important carbohydrate source. The objective of this research was to identify superior cassava variety candidate of selected cassava developed by of Research Centre Biotechnology, LIPI which has high adaptation capability and yield in Central Kalimantan peat land area. This research was conducted from July 2010 through May 2011 in Central Kalimantan Province (Kalampangan dan Pulang Pisau in total of area 1.5 and 2.25 ha respectively). There were three genotypes of cassava tested, i.e. Iding (high amylose), Gebang (low amylose), and Menti (high starch) compared to Adira 4 (35 ton/ha) and Darul Hidayah (102.1 ton/ha). The variables observed were plant height (cm) and tuber weight (grams). Observations were conducted at the age of 10 months. Data was analyzed using SPSS version 16.0. The results showed that the plant height and yield were higher in Pulang Pisau than Kalampangan even though the difference is not significant (P ≤ 0,05). Darul Hidayah has highest plant height and yield, 229.8 cm and 2271 g respectively among other genotypes/varieties grown at Pulang Pisau even though the value was not significant, whereas in Kalampangan, Darul Hidayah showed the lowest yield (670 g). Based on the data obtained, the Darul Hidayah variety can be cultivated by farmers in Pulang Pisau peat land to fulfill the availability of superior cassava seedlings for food, feed and industry. Keywords: Peat, tuber yield, plant height, liquid organic fertilizer, yam wood.
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Maciel, Maria Inês Sucupira, Vera Lúcia Arroxelas Galvão de Lima, Eufrásio Souza dos Santos, and Marilene da Silva Lima. "Effects of biofilm and refrigeration on acerola postharvest conservation." Revista Brasileira de Fruticultura 26, no. 1 (April 2004): 168–70. http://dx.doi.org/10.1590/s0100-29452004000100044.
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The objective of this work was to compare the effects of four different concentrations of cassava starch film and storage temperature on shelf life and the quality of a genotype of acerola from the Germplasm Active Bank (GAB) of Federal Rural University of Pernambuco, Brazil. Sound orange-reddish acerola fruits were washed with a chlorine solution (100 mg.L-1 active chlorine) and randomly distributed into different lots. The fruits were dipped for 3 min in a cassava starch suspension with concentrations of 1, 2, 3 and 4% (w/v) and the control without coating, stored at 10ºC (85% RH) and 22ºC (85% RH). The total soluble solids (TSS), pH, titratable acidity (TA) and ascorbic acid (AA) were determined at harvest and regular interval during storage. The use of cassava biofilm at 1% on acerola fruits maintained the highest ascorbic acid content and the temperature of 10ºC extended storage life. The fruits coated with 1 and 2% biofilm could be stored for a period up to 15 days at 10ºC, with acceptable quality characteristics.
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Fakir, MSA, M. Jannat, MG Mostafa, and H. Seal. "Starch and flour extraction and nutrient composition of tuber in seven cassava accessions." Journal of the Bangladesh Agricultural University 10, no. 2 (April 20, 2013): 217–22. http://dx.doi.org/10.3329/jbau.v10i2.14698.
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Cassava (Manihot esculenta Crantz) roots (tubers) are used as staple food. Starch extracted from tubers is widely utilized as raw materials in industries. Dry matter (DM) content, starch and flour extraction and proximate composition were investigated in seven cassava accessions (Coc-A1, Kh-A2, Cow-A3, Sa-A4, Me-A5, Va-A6 and Sy-A8.) in 2010- 2011. Leaf DM varied from 20.51% in Me-A5 to 29.01% in Sy-A8; that of stem from 27.24% in Va-A6 to 32.10% (average of Sy-A8, Me-A5 and Sa-A4); and that of tuber from 37.30% in Kh-A2 to 45.26% in Sy-A8. Starch was extracted by blending chopped tuber followed by decantation. Tubers were sliced, sun dried and milled into flour. Tuber starch content (fresh wt. basis) varied between 15.04% in Sy-A8 and 24.97% (average of Coc-A1 and Me-A5); that of peel from 4.54% in Va-A6 to 5.85% in Coc-A1. Crude protein varied from 1.80% (average of Kh-A2, Cow-A3 and Sy-A8) to 4.53% in Va-A6. Crude fiber content varied from 1.95% (average of Sa-A4 and Coc-A1) to 4.27% in Cow-A3. Cyanogens present in cassava plant escape as hydrogen cyanide (HCN) during harvesting and processing. Variation for HCN existed and it was 140.95 mg/kg fresh tuber (average of Sy-A8 and Coc-A1) to 546.0 mg/kg fresh tuber in Va-A6. There was no detectable HCN in the extracted flour and starch. It may be concluded that genetic variation for DM, starch, protein and HCN existed in seven cassava accessions, and Coc-A1 may be a better one due to its lower HCN, higher DM and starch content. DOI: http://dx.doi.org/10.3329/jbau.v10i2.14698 J. Bangladesh Agril. Univ. 10(2): 217-222, 2012
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Makame, M., M. O. Akoroda, and S. K. Hahn. "Effects of reciprocal stem grafts on cyanide translocation in cassava." Journal of Agricultural Science 109, no. 3 (December 1987): 605–8. http://dx.doi.org/10.1017/s0021859600081855.
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Cassava (Manihot esculenta Crantz) is a carbohydrate staple and cash crop for about 800 million people in the tropics. As food, its use is influenced by its content of potentially toxic cyanogenic glycosides that release hydrocyanic acid when enzymatically hydrolysed (Conn, 1969). Cyanide plays important role in the protection of cassava plants from attacks by animals and insect pests. The glycosides are synthesized mainly in leaves and translocated to all parts of the cassava plant (Bediako, Tapper & Pritchard, 1981). De Bruijn (1973) noted more than a 100% increase in the HCN content of stem bark above the incision after ringing, especially during the first 2 days, and a continued increase for at least 2 months. However, when leaves were removed, no such increase was observed. De Bruijn (1973) observed that younger plants showed a greater increase (165%) than older plants (65%). Leaf HCN did not increase after ringing, whereas root HCN decreased by about 20% in 2 weeks. The literature on cyanogenic glycoside translocation is relatively recent and limited. Consequently, the exploitation of the supposed direct relationship between leaf HCN and root HCN in the selection of low HCN clones in cassava breeding programmes needs to be carefully assessed. The assessment is complicated because the methods used by different workers for the determination of HCN vary in their efficiency. Thus, Cooke, Howland & Hahn (1978) found no correlation (r2 = 0·13) between leaf HCN and root HCN among 108 clones, yet over 88000 genotypes have been screened by analysis of leaf HCN, and leaf analysis has been used routinely in cassava improvement for 9 years (Hahn, 1983). Hahn also reported the range of HCN concentration within each leaf-picrate class (1 = 80, 2 = 80−200, 3 = 200 mg/100g fresh weight) but the ranges within each of these classes were too wide to enable effective selection for low HCN clones.
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Bester, Adriano Udich, Ivan Carvalho, José Antonio Gonzalez da Silva, Danieli Jacoboski Hutra, Natã Balssan Moura, Francine Lautenchleger, Adriano Hirsch Ramos, and Cristiano Dietrich Ferreira. "Positioning of cassava cultivars in space management and use of biostimulant." Agronomy Science and Biotechnology 6 (January 19, 2021): 1–15. http://dx.doi.org/10.33158/asb.r114.v6.2020.
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Cassava plays a fundamental role in food, economy, culture and in Brazilian society. Because it is a rustic crop, tolerant to drought and acidic soils, with high adaptive capacity and low production cost, it has become the main source of carbohydrate in much of the world. Due to this and other factors, the presumed work aims to evaluate four cassava genotypes, transplanted in two different densities with the use of a biostimulant for root growth. The present study was carried out in the experimental area of Regional University of Northwest Rio Grande do Sul, in the county of Augusto Pestana – RS, Brazil. The treatments consisted of three cassava cultivars, FEPAGRO-RS 13 Vassourinha, BRS CS01, Iapar - 19 Pioneira with two planting densities, 10 and 20 buds per linear meter, with and without the use of plant biostimulator Sturdy®. The evaluations were carried out throughout the crop cycle, post-harvest and processing. Where the following variables were evaluated: plants emerged at 30, 60 and 90 days, plants that tuberized, starch, plant height, stem diameter, total root length, lignified part of the root, commercial root length, mean root diameter, number of leaves, branching of the stems, roots that tuberized and not tuberized, mass of tuberized roots, fresh mass of commercial tuber with peel, fresh mass of commercial tuber without peel, mass of partially dry peeled tuber. Regarding the density, the one that showed a better performance in the productive character was the density 10 buds per linear meter, which presented the highest averages of the commercial fresh mass without peel. Taking into account the cultivars, the one with the highest fresh mass production was BRS CS01 and Iapar-19 Pioneira. However, FEPAGRO-RS 13 Vassourinha presented a higher percentage of starch. The application of the biostimulant of root growth showed significant effect mainly for the characteristics of productivity. The increase in the number of branches decreases the final productivity of the crop. Another point to consider, the larger the average root diameter, the greater the percentage of starch in the root. By the method of Annicchiarico it can be pointed out that in general the percentage of starch and the dry matter of the roots had a better behavior in density 10 with biostimulant and density 20 with biostimulant.
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Wasonga, Daniel O., Afrane Yaw, Jouko Kleemola, Laura Alakukku, and Pirjo S. A. Mäkelä. "Red-Green-Blue and Multispectral Imaging as Potential Tools for Estimating Growth and Nutritional Performance of Cassava under Deficit Irrigation and Potassium Fertigation." Remote Sensing 13, no. 4 (February 8, 2021): 598. http://dx.doi.org/10.3390/rs13040598.
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Cassava has high energy value and rich nutritional content, yet its productivity in the tropics is seriously constrained by abiotic stresses such as water deficit and low potassium (K) nutrition. Systems that allow evaluation of genotypes in the field and greenhouse for nondestructive estimation of plant performance would be useful means for monitoring the health of plants for crop-management decisions. We investigated whether the red–green–blue (RGB) and multispectral images could be used to detect the previsual effects of water deficit and low K in cassava, and whether the crop quality changes due to low moisture and low K could be observed from the images. Pot experiments were conducted with cassava cuttings. The experimental design was a split-plot arranged in a completely randomized design. Treatments were three irrigation doses split into various K rates. Plant images were captured beginning 30 days after planting (DAP) and ended at 90 DAP when plants were harvested. Results show that biomass, chlorophyll, and net photosynthesis were estimated with the highest accuracy (R2 = 0.90), followed by leaf area (R2 = 0.76). Starch, energy, carotenoid, and cyanide were also estimated satisfactorily (R2 > 0.80), although cyanide showed negative regression coefficients. All mineral elements showed lower estimation accuracy (R2 = 0.14–0.48) and exhibited weak associations with the spectral indices. Use of the normalized difference vegetation index (NDVI), green area (GA), and simple ratio (SR) indices allowed better estimation of growth and key nutritional traits. Irrigation dose 30% of pot capacity enriched with 0.01 mM K reduced most index values but increased the crop senescence index (CSI). Increasing K to 16 mM over the irrigation doses resulted in high index values, but low CSI. The findings indicate that RGB and multispectral imaging can provide indirect measurements of growth and key nutritional traits in cassava. Hence, they can be used as a tool in various breeding programs to facilitate cultivar evaluation and support management decisions to avert stress, such as the decision to irrigate or apply fertilizers.
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Shi, T., C. P. Li, J. F. Li, J. M. Cai, and G. X. Huang. "First Report of Leaf Spot Caused by Bipolaris setariae on Cassava in China." Plant Disease 94, no. 7 (July 2010): 919. http://dx.doi.org/10.1094/pdis-94-7-0919a.
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Cassava (Manihot esculenta Crantz) is an important food crop in tropical regions of China. Seventy percent of the cassava output is used for starch and ethanol production and it has become the base of food and bioenergy industries. In July 2009, a new leaf spot disease was found on cv. HuaNan205 from a cassava plantation in Danzhou, Hainan Province. Disease occurred on 50% or less of the plants. Initial symptoms were elliptical, chlorotic, and water-immersion lesions of 2 to 4 mm in diameter. These lesions became dry and yellow due to the progress of the disease. A brown halo was around the lesions, and in wet conditions, a dark gray mildew often appeared in the middle of the lesion. Diseased leaves turned yellow and the plants eventually became defoliated. The pathogen was isolated and pathogenicity was established by following Koch's postulates. Young, healthy, and fully expanded green leaves of Cassava cv. HuaNan205 were surface sterilized and then inoculated by spraying them with a suspension of conidia (1 × 105 conidia per ml) of the isolate. Sterile water was used as a control. The leaves were kept in a humid chamber at 28°C for 4 days, at which time similar symptoms to those described above were observed on the leaves. The pathogen was reisolated from inoculated leaves. Microscopic examination showed the conidiophores were fasciculate and brown, septate and straight, and the basal cell was enlarged and hemispherical. Well-developed conidia were long-obclavate, obtuse at both ends, straight, brown, with five to eight transverse septa, and measured 49.7 to 117.1 × 13.3 to 17.2 μm. Genomic DNA of this isolate was extracted with a cetyltrimethylammoniumbromide protocol, and amplification and sequencing of the internal transcribed spacer (ITS) region of rDNA was performed with procedures outlined by Cooke et al. (2). The sequence of the region was deposited in GenBank (Accession No. GU290228). Comparison of the sequences available in the GenBank database revealed that the current ITS sequence differs by three base pairs from two Bipolaris setariae isolates (EF452444 and FJ606786). Morphological identification and sequence analysis of ITS rDNA showed that the pathogen was B. setariae. B. setariae is one of the most important pathogens of lawn grass, gramineous crops, and other plants (1,3). However, no leaf spot disease caused by B. setariae has been recorded previously on cassava in China or elsewhere. References: (1) P. Busey. Crop Sci. 43:1899, 2003. (2) D. E. L. Cooke et al. Fungal Genet. Biol. 30:17, 2000. (3) H. D. Wells and W. W. Hanna. Phytopathology 78:1179, 1988.
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Guo, H., C. P. Li, T. Shi, C. J. Fan, and G. X. Huang. "First Report of Phytophthora palmivora Causing Root Rot of Cassava in China." Plant Disease 96, no. 7 (July 2012): 1072. http://dx.doi.org/10.1094/pdis-09-11-0780-pdn.
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Cassava (Manihot esculenta) is an economically important crop grown widely in South China. Seventy percent of the cassava grown is used for starch and ethanol production and it has become the foundation of local food and bioenergy systems. In November 2010, a new root rot disease was found on cv. HuaNan205 from a cassava plantation in Danzhou, Hainan Province. Disease occurred on 30% or less of the plants. Initially, the upper leaves wilted at noon and recovered in the evening. Eventually, infected plants no longer recovered and the whole plant wilted and died. Root rot symptoms consisting of irregular brown patches occurred on the tuberous roots. Symptomatic root rot tissue was cut into 1-cm pieces, washed in distilled water, and soaked in a solution of 1% sodium hypochlorite for 3 min. A subsection was cut from each sterilized piece, placed on a plate of V8 agar medium, and incubated at 28°C for 7 days. Pathogenicity was established by following Koch's postulates. In July 2011, 10 plants of cassava cv. HuaNan205 were selected from a disease-free plantation in Danzhou. The pathogen was cultivated on V8 agar at 28°C for 14 days. Four holes were established 15 cm from the base of the cassava plants. Five plants were inoculated with 100 mL of the mycelial suspension in each of the four spots and covered by soil. The other five plants were treated with sterile water as control. Plants were maintained for 4 months. All five of the inoculated plants wilted and two died, while the control plants grew normally. Symptoms similar to the original root lesions were observed on tuberous roots of inoculated plants, while only scars formed on tuberous roots of control plants. The pathogen was reisolated from the lesions of inoculated plants. Microscopic examination showed the sporangia as papillate and ovoid with the widest part close to the base. They were easily washed off and each detached sporangium contained a short pedicel 1.2 to 6.9 μm long, average 2.9 μm. Chlamydospores were readily observed on diseased roots and observed in pure cultures on V8 agar. Morphological characteristics of the specimen were similar to the descriptions for Phytophthora palmivora (2). Genomic DNA of this isolate was extracted with a cetyltrimethylammoniumbromide protocol (3) from mycelium and used as a template for amplification of the internal transcribed spacer (ITS) region of rDNA with primer pair ITS1/ITS4 (1). The sequence (GenBank Accession No. HE580279) exactly matched several sequences (e.g., GenBank Accession Nos. HQ237481.1, AY745750, and AY745751) of P. palmivora. To our knowledge, this is the first report of root rot caused by P. palmivora on cassava in China. References: (1) D. E. L. Cooke et al. Fungal Genet. Biol. 30:17, 2000. (2) D. C. Erwin and O. K. Ribeiro. Phytophthora Diseases Worldwide. The American Phytopathological Society, St. Paul, MN, 1996. (3) J. R. Xu et al. Genetics 143:175, 1996.
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Bowrin, Valerie, Judy Rouse-Miller, Fedora Sutton, and Grace Sirju-Charran. "Formamide-based RNA Isolation at Above ZeroTemperatures from High Starch Cassava Tubers." Phytochemical Analysis 24, no. 1 (July 23, 2012): 93–96. http://dx.doi.org/10.1002/pca.2390.
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Sudarmonowati, Enny, N. Sri Hartati, and Supatmi Supatmi. "Enhancement Of Yield, Starch, And Amylose Content Of Two Indonesian Cassava Genotypes By Producing Gamma Irradiated-Induced Mutants." ANNALES BOGORIENSES 24, no. 2 (January 5, 2021): 95. http://dx.doi.org/10.14203/ann.bogor.2020.v24.n2.95-105.
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As an important carbohydrate-source food crop and raw materials of starch-based industries, accelerated cassava breeding has been extensively pursued through various procedures, among others, is mutant induction using gamma irradiation combined with molecular marker. Iding, a local genotype cassava has been compared with a national variety Adira 4 for its yield attributes following gamma irradiation at various dose levels ( 2, 20, 30, and 50 krad). The mutation rate was conducted based on morphological characteristics and identification using RAPD by employing random primer OPB-10. Evaluation was conducted on mutant lines producing high potential yield, high starch and amylose contents. The fourth generation of irradiated Adira 4 (2 and 30 krad) and Iding (2 krad) exhibited higher yield and amylose content as compared to their representative controls. The yield of 30 krad irradiated Adira 4 indicated by mean tuber weight per individual could reach more than twice of that of control and almost 1.5 times of that irradiated with 2 krad. The levels of amylose content of all irradiated ones, regardless of genotypes and gamma irradiation doses (2 and 30 krad) were higher than that of control. In most parameters, irradiated Adira 4 was superior to irradiated Iding.
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Romagnolli, Cassiano MN, Gabriela P. Leite, Tiago AR Rodrigues, and Carolina L. Morelli. "Blend of cassava starch and high-density polyethylene with green tea for food packaging." Polymers from Renewable Resources 11, no. 1-2 (February 2020): 3–14. http://dx.doi.org/10.1177/2041247920952641.
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Plastic packagings are widely used for several food products. Considering the relatively short service lifetime of this application, it is important to perceive in the search of eco-friendly alternatives to this market, such as polymers from renewable sources, as thermoplastic starch and “green” polyethylene. The incorporation of an antibacterial agent to the packaging can extend food shelf life. Camellia sinensis is a plant with known antibacterial properties used in the preparation of “green tea.” In the present work, green tea was incorporated to a blend of cassava thermoplastic starch and high-density polyethylene (HDPE) by melt extrusion, aiming application as active packaging. Films were obtained by thermopressing and characterized through infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy and tensile tests. Their antibacterial properties were evaluated against Staphylococcus aureus and Escherichia coli. The results indicated that the material developed has potential for food packaging applications. Moreover, the methodology applied for green tea incorporation in the Starch/HDPE films can be extended for many extracts from natural components, contributing to the advancement of research in the development of active packaging for food products. To the best of our knowledge, no previous work studied the properties of starch/HDPE blend with green tea.
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Hazrati, K. Z., S. M. Sapuan, M. Y. M. Zuhri, and R. Jumaidin. "Extraction and Characterization of Potential Biodegradable Materials Based on Dioscorea hispida Tubers." Polymers 13, no. 4 (February 15, 2021): 584. http://dx.doi.org/10.3390/polym13040584.
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This study was driven by the stringent environmental legislation concerning the consumption and utilization of eco-friendly materials. Within this context, this paper aimed to examine the characteristics of starch and fibres from the Dioscorea hispida tuber plant to explore their potential as renewable materials. The extraction of the Dioscorea hispida starch and Dioscorea hispida fibres was carried out and the chemical composition, physical, thermal, morphological properties, and crystallinity were studied. The chemical composition investigations revealed that the Dioscorea hispida starch (DHS) has a low moisture t (9.45%) and starch content (37.62%) compared to cassava, corn, sugar palm, and arrowroot starches. Meanwhile, the Dioscorea hispida fibres (DHF) are significantly low in hemicellulose (4.36%), cellulose (5.63%), and lignin (2.79%) compared to cassava, corn hull and sugar palm. In this investigation the chemical, physical, morphological and thermal properties of the Dioscorea hispida fibre and Dioscorea hispida starch were examined by chemical composition investigation, scanning electron microscopy (SEM), particle size distribution, thermogravimetric analysis (TGA), X-ray powder diffraction (XRD), and Fourier transform infrared (FTIR), respectively. It was found that Dioscorea hispida waste is promising alternative biomass and sustainable material with excellent potential as a renewable filler material for food packaging applications.
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Garrett, K. A., K. F. Andersen, F. Asche, R. L. Bowden, G. A. Forbes, P. A. Kulakow, and B. Zhou. "Resistance Genes in Global Crop Breeding Networks." Phytopathology® 107, no. 10 (October 2017): 1268–78. http://dx.doi.org/10.1094/phyto-03-17-0082-fi.
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Resistance genes are a major tool for managing crop diseases. The networks of crop breeders who exchange resistance genes and deploy them in varieties help to determine the global landscape of resistance and epidemics, an important system for maintaining food security. These networks function as a complex adaptive system, with associated strengths and vulnerabilities, and implications for policies to support resistance gene deployment strategies. Extensions of epidemic network analysis can be used to evaluate the multilayer agricultural networks that support and influence crop breeding networks. Here, we evaluate the general structure of crop breeding networks for cassava, potato, rice, and wheat. All four are clustered due to phytosanitary and intellectual property regulations, and linked through CGIAR hubs. Cassava networks primarily include public breeding groups, whereas others are more mixed. These systems must adapt to global change in climate and land use, the emergence of new diseases, and disruptive breeding technologies. Research priorities to support policy include how best to maintain both diversity and redundancy in the roles played by individual crop breeding groups (public versus private and global versus local), and how best to manage connectivity to optimize resistance gene deployment while avoiding risks to the useful life of resistance genes. [Formula: see text] Copyright © 2017 The Author(s). This is an open access article distributed under the CC BY 4.0 International license .
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Kimura, Mieko, Cintia N. Kobori, Delia B. Rodriguez-Amaya, and Penelope Nestel. "Screening and HPLC methods for carotenoids in sweetpotato, cassava and maize for plant breeding trials." Food Chemistry 100, no. 4 (January 2007): 1734–46. http://dx.doi.org/10.1016/j.foodchem.2005.10.020.
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Sudarmonowati, Enny, and Hani Fitriani. "Growth and yield performance of four generations of high amylose transgenic Adira 4 cassava." Ilmu Pertanian (Agricultural Science) 6, no. 1 (January 6, 2021): 54. http://dx.doi.org/10.22146/ipas.59418.
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Attempt to improve cassava starch quality have been conducted by producing the first high amylose transgenic Adira 4 Indonesian cassava. This atudy aimed to evaluate agronomic performances of transgenic plants, assessment of four generations in specific containment screenhouse and limited field trial were conducted with improved method. The survival and growth rate of cuttings was enhanced by applying growth regulator such as Atonik and improving planting procedures. The growth and yield performances were measured by comparing related variables across four generations of selected lines. Dipping in 1 mL.L-1 Atonik solution with shading in the early planting were only required when the plant growth was poor and not favourable soil and weather. The result showed that survival of third and fourth generations of Lines A55-5 and A55-15 could reach 100 % when it was treated with Atonik. According to 14 lines assessed, Line A55-5 was one of the promising ones due to its growth, multiplication performance and almost double amylose content compared to wild type. Meanwhile, Line A55-12 showed the highest yield. The finding will benefit functional food industries when appropriate gene construct and approaches were applied for better growth and yield results.
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Ding, Zehong, Lili Fu, Weiwei Tie, Yan Yan, Chunlai Wu, Jing Dai, Jiaming Zhang, and Wei Hu. "Highly dynamic, coordinated, and stage-specific profiles are revealed by a multi-omics integrative analysis during tuberous root development in cassava." Journal of Experimental Botany 71, no. 22 (August 10, 2020): 7003–17. http://dx.doi.org/10.1093/jxb/eraa369.
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Abstract Cassava (Manihot esculenta) is an important starchy root crop that provides food for millions of people worldwide, but little is known about the regulation of the development of its tuberous root at the multi-omics level. In this study, the transcriptome, proteome, and metabolome were examined in parallel at seven time-points during the development of the tuberous root from the early to late stages of its growth. Overall, highly dynamic and stage-specific changes in the expression of genes/proteins were observed during development. Cell wall and auxin genes, which were regulated exclusively at the transcriptomic level, mainly functioned during the early stages. Starch biosynthesis, which was controlled at both the transcriptomic and proteomic levels, was mainly activated in the early stages and was greatly restricted during the late stages. Two main branches of lignin biosynthesis, coniferyl alcohol and sinapyl alcohol, also functioned during the early stages of development at both the transcriptomic and proteomic levels. Metabolomic analysis further supported the stage-specific roles of particular genes/proteins. Metabolites related to lignin and flavonoid biosynthesis showed high abundance during the early stages, those related to lipids exhibited high abundance at both the early and middle stages, while those related to amino acids were highly accumulated during the late stages. Our findings provide a comprehensive resource for broadening our understanding of tuberous root development and will facilitate future genetic improvement of cassava.
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López, Antonio José, and Norbey Marín. "Identificación de caracteres de preferencia en variedades de yuca por parte de usuarios de la cadena producción-consumo en la región Caribe colombiana." Corpoica Ciencia y Tecnología Agropecuaria 8, no. 2 (January 7, 2008): 42. http://dx.doi.org/10.21930/rcta.vol8_num2_art:93.
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<p>La incorporación de los criterios de los usuarios del conocimiento en diferentes eslabones de la cadena producción-consumo ha sido documentada ampliamente en su eslabón primario y con relación al fitomejoramiento. No obstante, la vinculación al proceso de investigación de conceptos procedentes de otros eslabones podría garantizar mejor impacto en la productividad. La presente investigación tuvo dos objetivos: 1) identificar los caracteres preferidos por los usuarios del conocimiento en los diferentes eslabones de la cadena agroindustrial de la yuca y, 2) incorporar dichos caracteres en el diseño de nuevas variedades. Los agricultores e intermediarios evaluaron clones en fincas, los fitomejoradores en el centro de investigación, los procesadores (picadoressecadores) en las plantas de secado y los consumidores de yuca fresca en los hogares. Los diversos criterios y caracteres fueron registrados y codificados a fin de realizar análisis de correspondencia múltiple. Mientras los agricultores hombres lograron una buena diferenciación entre los mejores clones por sus atributos, las mujeres tuvieron menor diferenciaron debido a que utilizaron categorías similares en la mayoría de los caracteres. La correspondencia entre los nueve mejores clones seleccionados por los fitomejoradores en las cinco localidades y sus caracteres asociados, permitió identificar tres grupos de clones con caracteres deseables y dos clones con caracteres no muy deseables. Los caracteres comunes entre los mejores clones evaluados por consumidores de hogar se asociaron con el sabor, el color de la pulpa, la consistencia y el contenido de harina. El análisis de caracteres comunes entre todos los evaluadores indicó que un eje lo determinaron los consumidores y el otro los fitomejoradores. Los resultados permitieron concluir que un contenido alto de almidón fue el rasgo más determinante en la selección de un buen clon para secado natural; para los almidoneros, la relación yuca fresca/almidón seco y el color blanco de la cutícula fueron los rasgos más importantes. Finalmente, se identificaron caracteres comunes entre procesadores, consumidores, investigadores, agricultores hombres y agricultores mujeres. </p><p> </p><p><strong>Identification of preferred traits of cassava varieties by users in the production-consumption chain at the Colombian Caribbean region</strong></p><p>The incorporation of end-user criteria in different links of the production-consumption chain has been broadly documented in the first link and with relation to plant breeding. However incorporating concepts in the research process from other links could guarantee a greater impact on productivity. The purpose of this research was two fold: 1) to identify preferred knowledge users characteristics in the different links of the cassava agrochain, and 2) to incorporate these traits in the design of new varieties. Evaluation of cassava clones was done by farmers and middlemen on farms, plant breeders evaluated at research centers, processors evaluated at chipping and drying plants and fresh cassava consumers at the household level. Through open interview, diverse opinions and traits were recorded and encoded to perform a multiple correspondence analysis. While male farmers achieved a proper differentiation between the best clones by their characteristics, women had less differentiation because they used more similar categories for most traits. At five localities, plant breeders’ selection of the best nine clones and their associated characters allowed for the identification of three groups of clones with desirable traits and two clones with undesirables characteristics. Common traits among the best clones evaluated by household consumers were associated with taste, pulp color, consistency and starch content. Analyses of common traits among all evaluators indicated that one axis was established by consumers and another by plant breeders. The results indicated that high starch content was the determining feature in clone selection for natural dryers, while for starch producers the most important traits were fresh weight/dry starch ratio and white skin color. At the end, common criteria were identified among dryers, household consumers, researchers and farmers gender. </p>
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Damat, Damat, Rista Anggriani, Roy Hendroko Setyobudi, and Peeyush Soni. "DIETARY FIBER AND ANTIOXIDANT ACTIVITY OF GLUTEN-FREE COOKIES WITH COFFEE CHERRY FLOUR ADDITION." Coffee Science 14, no. 4 (December 9, 2019): 493. http://dx.doi.org/10.25186/cs.v14i4.1625.
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<p>Coffee husk and coffee pulp are by-product of coffee fruit and bean processing, can be considered as potential functional ingredients for food production as coffee cherry flour (CCF). The CCF contains a lot of carbohydrates, proteins, caffeine, tannins, and polyphenols. In this study, CCF was combined with modified arrowroot starch (MAS) and modified cassava flour (MOCAF) into cookies and improvement on the physical, chemical, and nutraceutical properties of the cookies were studied. The cookies consisted of 20 % of MOCAF and 80 % mixed of modified arrowroot starch and CCF in five levels (80 %:0 %; 75 %:5 %; 70 %:10 %; 65 %:15 %; 60 %:20 %) and objective physical, chemical, and nutraceutical properties of the cookies were assessed. The results showed that the total dietary fiber content was enhanced from 11.69 % to 19.48 % with a high proportion of 20 % CCF. The cookies added with CCF displayed enhanced antioxidant activity. Acceptable cookies were obtained by adding 5 % CCF. Thus, the results implied that cookies with CCF addition obtained dietary fiber enriched cookies with improved antioxidant activity.</p>
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Salwa, H. N., S. M. Sapuan, M. T. Mastura, and M. Y. M. Zuhri. "Application of Shannon’s entropy-analytic hierarchy process (AHP) for the selection of the most suitable starch as matrix in green biocomposites for takeout food packaging design." BioResources 15, no. 2 (April 10, 2020): 4065–88. http://dx.doi.org/10.15376/biores.15.2.4065-4088.
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Starch is a natural polymer and eligible for short-term, single-use food packaging applications. Nevertheless, different starches have different features and properties determined by their botanical plant origins. This paper presents an approach that combines Shannon’s entropy and the Analytic Hierarchy Process method to aid the selection process of starch as matrix in green biocomposites for takeout food packaging design. The proposed selection system ranks alternative starches in terms of the key design elements, i.e. strength, barrier property, weight, and cost. Shannon’s entropy established corresponding weight values for the indicators selected. Six starches: wheat, maize, potato, cassava, sago, and rice were appraised using gathered data from the literature to determine their suitability as a more sustainable option. This study found that sago starch obtained the highest priority score of 26.8%, followed by rice starch (20.2%). Sensitivity analysis was then carried out to further verify the results; sago starch was at the top rank for five of six different scenarios tested. The results showed that sago starch is the starch that can best satisfy the design requirements. Despite the results attained, the selection framework used could be enhanced with a more comprehensive attributes assessment and extensive dataset.
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West, Thomas P. "Production of the Polysaccharide Curdlan by Agrobacterium species on Processing Coproducts and Plant Lignocellulosic Hydrolysates." Fermentation 6, no. 1 (January 24, 2020): 16. http://dx.doi.org/10.3390/fermentation6010016.
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This review examines the production of the biopolymer curdlan, synthesized by Agrobacterium species (sp.), on processing coproducts and plant lignocellulosic hydrolysates. Curdlan is a β-(1→3)-D-glucan that has various food, non-food and biomedical applications. A number of carbon sources support bacterial curdlan production upon depletion of nitrogen in the culture medium. The influence of culture medium pH is critical to the synthesis of curdlan. The biosynthesis of the β-(1→3)-D-glucan is likely controlled by a regulatory protein that controls the genes involved in the bacterial production of curdlan. Curdlan overproducer mutant strains have been isolated from Agrobacterium sp. ATCC 31749 and ATCC 31750 by chemical mutagenesis and different selection procedures. Several processing coproducts of crops have been utilized to support the production of curdlan. Of the processing coproducts investigated, cassava starch waste hydrolysate as a carbon source or wheat bran as a nitrogen source supported the highest curdlan production by ATCC 31749 grown at 30 °C. To a lesser extent, plant biomass hydrolysates have been explored as possible substrates for curdlan production by ATCC 31749. Prairie cordgrass hydrolysates have been shown to support curdlan production by ATCC 31749 although a curdlan overproducer mutant strain, derived from ATCC 31749, was shown to support nearly double the level of ATCC 31749 curdlan production under the same growth conditions.
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Foley, Jennifer K., Kristina D. Michaux, Bho Mudyahoto, Laira Kyazike, Binu Cherian, Olatundun Kalejaiye, Okonkwo Ifeoma, et al. "Scaling Up Delivery of Biofortified Staple Food Crops Globally: Paths to Nourishing Millions." Food and Nutrition Bulletin 42, no. 1 (February 17, 2021): 116–32. http://dx.doi.org/10.1177/0379572120982501.
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Background: Micronutrient deficiencies affect over one quarter of the world’s population. Biofortification is an evidence-based nutrition strategy that addresses some of the most common and preventable global micronutrient gaps and can help improve the health of millions of people. Since 2013, HarvestPlus and a consortium of collaborators have made impressive progress in the enrichment of staple crops with essential micronutrients through conventional plant breeding. Objective: To review and highlight lessons learned from multiple large-scale delivery strategies used by HarvestPlus to scale up biofortification across different country and crop contexts. Results: India has strong public and private sector pearl millet breeding programs and a robust commercial seed sector. To scale-up pearl millet, HarvestPlus established partnerships with public and private seed companies, which facilitated the rapid commercialization of products and engagement of farmers in delivery activities. In Nigeria, HarvestPlus stimulated the initial acceptance and popularization of vitamin A cassava using a host of creative approaches, including “crowding in” delivery partners, innovative promotional programs, and development of intermediate raw material for industry and novel food products. In Uganda, orange sweet potato (OSP) is a traditional subsistence crop. Due to this, and the lack of formal seed systems and markets, HarvestPlus established a network of partnerships with community-based nongovernmental organizations and vine multipliers to popularize and scale-up delivery of OSP. Conclusions: Impact of biofortification ultimately depends on the development of sustainable markets for biofortified seeds and products. Results illustrate the need for context-specific, innovative solutions to promote widespread adoption.
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Bouis, Howarth E. "Micronutrient fortification of plants through plant breeding: can it improve nutrition in man at low cost?" Proceedings of the Nutrition Society 62, no. 2 (May 2003): 403–11. http://dx.doi.org/10.1079/pns2003262.
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Can commonly-eaten food staple crops be developed that fortify their seeds with essential minerals and vitamins? Can farmers be induced to grow such varieties? If so, would this result in a marked improvement in human nutrition at a lower cost than existing nutrition interventions? An interdisciplinary international effort is underway to breed for mineral- and vitamin-dense varieties of rice, wheat, maize, beans and cassava for release to farmers in developing countries. The biofortification strategy seeks to take advantage of the consistent daily consumption of large amounts of food staples by all family members, including women and children as they are most at risk for micronutrient malnutrition. As a consequence of the predominance of food staples in the diets of the poor, this strategy implicitly targets low-income households. After the one-time investment is made to develop seeds that fortify themselves, recurrent costs are low and germplasm may be shared internationally. It is this multiplier aspect of plant breeding across time and distance that makes it so cost-effective. Once in place, the biofortified crop system is highly sustainable. Nutritionally-improved varieties will continue to be grown and consumed year after year, even if government attention and international funding for micronutrient issues fades. Biofortification provides a truly feasible means of reaching malnourished populations in relatively remote rural areas, delivering naturally-fortified foods to population groups with limited access to commercially-marketed fortified foods that are more readily available in urban areas. Biofortification and commercial fortification are, therefore, highly complementary. Breeding for higher trace mineral density in seeds will not incur a yield penalty. Mineral-packed seeds sell themselves to farmers because, as recent research has shown, these trace minerals are essential in helping plants resist disease and other environmental stresses. More seedlings survive and initial growth is more rapid. Ultimately, yields are higher, particularly in trace mineral-‘deficient’ soils in arid regions.
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Wiebe, Keith, Timothy B. Sulser, Shahnila Dunston, Mark W. Rosegrant, Keith Fuglie, Dirk Willenbockel, and Gerald C. Nelson. "Modeling impacts of faster productivity growth to inform the CGIAR initiative on Crops to End Hunger." PLOS ONE 16, no. 4 (April 15, 2021): e0249994. http://dx.doi.org/10.1371/journal.pone.0249994.
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In 2017–2018, a group of international development funding agencies launched the Crops to End Hunger initiative to modernize public plant breeding in lower-income countries. To inform that initiative, USAID asked the International Food Policy Research Institute and the United States Department of Agriculture’s Economic Research Service to estimate the impacts of faster productivity growth for 20 food crops on income and other indicators in 106 countries in developing regions in 2030. We first estimated the value of production in 2015 for each crop using data from FAO. We then used the IMPACT and GLOBE economic models to estimate changes in the value of production and changes in economy-wide income under scenarios of faster crop productivity growth, assuming that increased investment will raise annual rates of yield growth by 25% above baseline growth rates over the period 2015–2030. We found that faster productivity growth in rice, wheat and maize increased economy-wide income in the selected countries in 2030 by 59 billion USD, 27 billion USD and 21 billion USD respectively, followed by banana and yams with increases of 9 billion USD each. While these amounts represent small shares of total GDP, they are 2–15 times current public R&D spending on food crops in developing countries. Income increased most in South Asia and Sub-Saharan Africa. Faster productivity growth in rice and wheat reduced the population at risk of hunger by 11 million people and 6 million people respectively, followed by plantain and cassava with reductions of about 2 million people each. Changes in adequacy ratios were relatively large for carbohydrates (already in surplus) and relatively small for micronutrients. In general, we found that impacts of faster productivity growth vary widely across crops, regions and outcome indicators, highlighting the importance of identifying the potentially diverse objectives of different decision makers and recognizing possible tradeoffs between objectives.
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Saleha, Sitti, Nurdin Saidi, Saiful ., Murniana ., Saida Rasnovi, and Teuku M. Iqbalsyah. "NUTRITIONAL COMPOSITION OF DIOSCOREA HISPIDA FROM DIFFERENT LOCATIONS AROUND LEUSER ECOSYSTEM AREA." Jurnal Natural 18, no. 1 (February 1, 2018): 1–6. http://dx.doi.org/10.24815/jn.v18i1.8504.
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Proximate analysis of Dioscorea hispida tubers, collected from five locations around Leuser ecosystem in Aceh Province, showed variations amongst samples. Standard AOAC method for proximate analysis of the fresh weight showed that the water content varied between 15.8 - 37.8%, crude protein 1.13 -6.20%, crude lipid 1.99 - 9.36% and ash 0.29 - 1.24%. The total carbohydrate was high, i.e. between 58.3 -71.9%. The main mineral was phosphorus, with a value of 11.7 - 46.9 mg/100g. These variations could be due to soil, climate and weather factors, as well as postharvest handling. Phytochemical tests showed that all of the samples contained alkaloids and terpenoids. One of the samples (LP) also contained phenol and steroid. The high cyanide content in the tubers (379 - 739 ppm) was easily removed by repeated washing. The cyanide level dropped significantly after the 3rd wash. Information on nutritional content in D. hispida is essential for planning its utilization. Increasing the economic value of D. hispida is expected to attract people around the Leuser ecosystem to cultivate and utilize it, thereby reducing illegal forest encroachment.Keywords: Dioscorea hispida, proximate, Leuser, janeng, gadung, starchREFERENCESBarton H 2014 Yams: Origins and Development, Encyclopaedia of Global Archaeology, p 7943-7947, (Springer. DOI 10.1007/978-1-4419-0465-2_2193).Obidiegwu J E and Akpabio E M 2017 The Geography of Yam Cultivation in Southern Nigeria: Exploring Its Social Meanings and Cultural Functions J. Ethnic Foods 4 28-35.Chandrasekara A and Kumar T J 2016 Roots and Tuber Crops as Functional Foods: A Review on Phytochemical Constituents and Their Potential Health Benefits Intl. J. Food Sci. 2016 1-15.Kumar S, Das G, Shin H-S and Patra J K 2017 Dioscorea spp. (A Wild Edible Tuber): A Study on Its Ethnopharmacological Potential and Traditional Use by the Local People of Similipal Biosphere Reserve India Front. Pharmacol. 8 52.Lin J Y, Lu S, Liou Y L and Liou H L 2006a Antioxidant and Hypolipidaemic Effects of a Novel Yam–boxthorn Noodle in an In Vivo Murine Model Food Chem. 94 377–384.Lin J Y, Lu S, Liou Y L and Liou H L, 2006b, Increased IgA and IgG Serum Levels Using a Novel Yam–boxthorn Noodle in a BALB/c Mouse Model Food Chem. Toxicol. 44 170–178.Bhandari M R and Kawabata J 2004 Organic Acid, Phenolic Content and Antioxidant Activity of Wild Yam (Dioscorea spp.) Tubers of Nepal Food Chem. 88 163–168.Lin J T and Yang D J 2008 Determination of Steroidal Saponins in Different Organs of Yam (Dioscorea pseudojaponica Yamamoto) Food Chem. 108 1068–1074.AOAC 1984. Official Methods of Analysis. Washington DC: Association of Official Analytical Chemists.Harborne J B 1984 Phytochemical Method 2nd ed. (London: Chapman and Hall Ltd.)Bhandari M R, Kasai T and Kawabata J 2003 Nutritional Evaluation of Wild Yam (Dioscorea spp.) Tubers of Nepal Food Chem. 82 619–623.Adepoju O T, Boyejo O and Adeniji P O 2017 Nutrient and Antinutrient Composition of Yellow Yam (Dioscorea cayenensis) Products Data in Brief 11 428–431.Wu Z-G, Jiang W, Nitin M, Bao X-Q, Chen S-L and Tao Z-M 2016 Characterizing Diversity Based on Nutritional and Bioactive Compositions of Yam Germplasm (Dioscorea spp.) Commonly Cultivated in China J. Food Drug Anal. 24 367 – 375.Udensi E A, Osebele H O and Iweala O O 2008 The Investigation of Chemical Composition and Functional Properties of Water Yam (Dioscorea alata): Effect of Varietal Differences Pakistan J. Nutrition 7(2) 342-344.Hornick S B 1992 Factors Affecting the Nutritional Quality of Crops Am. J. Altern. Agric. 7 (Special Issue on Soil Quality) 63-68.Lewicki P P 2004 Water as the Determinant of Food Engineering Properties, A Review J. Food Eng. 61 483–495Yeh A-I, Chan T-Y and Chuang G C-C 2009 Effect of Water Content and Mucilage on Physico-chemical Characteristics of Yam (Discorea alata Purpurea) Starch J. Food Eng. 95 106–114.McPherson E and Jane J 1999 Comparison of Waxy Potato with Other Root And Tuber Starches Carbohydr. Polym. 40 57–70.Freitas R A, Paula R C, Feitosa J P A, Rocha S and Sierakowski M R 2004 Amylose Contents, Rheological Properties and Gelatinization Kinetics of Yam (Dioscorea alata) and cassava (Manihot utilissima) starches Carbohydr. Polym. 55 3–8.Barsby T L, Donald A M, Frazier P J, Donald A M, Perry P A and Waigh T A 2001 The Impact of Internal Granule Structure on Processing and Properties in Starch: Advances in Structure and Function p 45-52 (Royal Society of Chemistry, http://dx.doi.org/10.1039/9781847551917-00045)Tattiyakul J, Naksriarporn T and Pradipasena P 2012 X-ray Diffraction Pattern and Functional Properties of Dioscorea hispida Dennst Starch Hydrothermally Modified at Different Temperatures Food Bioproc. Technol. 5 964–971.Savikin-Fodulovic K, Grubisic D, Culafic L, Menkovic N and Ristic M 1998 Diosgenin and Phytosterols Content in Five Callus Lines of Dioscorea balcanica Plant Sci. 135 63–67.Cushnie T. P. T, Cushnie B and Lamb A J 2014 Alkaloids: An Overview of Their Antibacterial, Antibiotic-enhancing and Antivirulence Activities Int. J. Antimicrob. Agents 44 (5) 377-386.Tholl D 2015 Biosynthesis and Biological Functions of Terpenoids in Plants, Biotechnology of Isoprenoids p 63-106 Part of the Advances in Biochemical Engineering/Biotechnology book series (ABE, volume 148).Nagata K, Aistrup G L, Honda H, Shono T and Narahashi T 1999 Modulation of the Nicotinic Acetylcholine Receptor by Dioscorine in Clonal Rat Phaeochlomocytoma (PC12) Cells Pestic. Biochem. Physiol. 64 (3) 157–165.Bhandari M R and Kawabata J 2005 Bitterness and Toxicity in Wild Yam (Dioscorea spp.) Tubers of Nepal Plant Foods Hum. Nutr. 60 129–135, 2005.White W L B , Arias-Garzon D I, McMahon J M and Sayre R 1998 Cyanogenesis in Cassava: The Role of Hydroxynitrile Lyase in Root Cyanide Production Plant Physiol. 116 1219-25.Kumoro A C and Hartati I. 2015 Microwave Assisted Extraction of Dioscorin from Gadung (Dioscorea hispida Dennst) Tuber Flour, Procedia Chem. 14 47 – 55.
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Aiman, Syahrul. "PERKEMBANGAN TEKNOLOGI DAN TANTANGAN DALAM RISET BIOETANOL DI INDONESIA." Jurnal Kimia Terapan Indonesia 16, no. 2 (December 10, 2014): 108–17. http://dx.doi.org/10.14203/jkti.v16i2.16.
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Bioetanol merupakan andalan untuk mengurangi penggunaan BBM non diesel untuk transportasi. Penelitian untuk mencari bahan baku dan proses yang ekonomis serta ramah lingkungan menjadi kegiatan riset di berbagai Negara, terutama sejak terjadinya krisis BBM akibat peningkatan konsumsi BBM diseluruh dunia diawal tahun 1970an.Berdasarkan bahan baku yang dipakai, bioetanol dikelompokkan menjadi bioetanol generasi pertama yang dibuat dari gula, atau pati, dan generasi kedua adalah yang dibuat dari lignoselulosa, disebut sebagai Etanol Selulosa. Generasi ketiga dibuat dari alga disebut sebagai Etanol Alga, dan generasi keempat dibuat dari bahan hasil modifikasi genetika atau bahan lainnya, disebut sebagai Advanced Bioethanol dalam kelompok Advanced Biofuels.Indonesia, sebagai Negara beriklim tropis, memiliki berbagai tanaman penghasil pati, lignoselulosa, alga dan berbagai limbah organik untuk pembuatan bioetanol.Industri bioetanol di Indonesia masih memanfaatkan komoditi pangan seperti ubi kayu dan molase tebu sebagai bahan baku, sedangkan lembaga litbang dan perguruan tinggi sudah melakukan penelitian membuat bioetanol generasi kedua maupun ketiga. Pemerintah Indonesia berupaya untuk meningkatkan penggunaan bioetanol sebagai campuran bahan bakar kendaraan non diesel sampai mencapai 15 % etanol dalam campuran (E-15) pada tahun 2025. Dibanyak Negara, pemanfaatan etanol untuk bahan bakar kendaraan sudah bervariasi dari campuran E-10 sampai dengan E-85.Tinjauan ini mengungkap perkembangan teknologi pada setiap generasi, dan mengindikasikan tantangan yang dihadapi lembaga litbang di dalam negeri dalam mengembangkan teknologi pembuatan bioetanol dari biomasa lokal. Area penelitian yang prospektif dalam bidang ini juga dikemukakan.Kata Kunci : bioetanol, molase tebu, generasi, perkembangan teknologi, tantangan riset. Bioethanol is a potential energy source to reduce gasoline utilization for transportation. Research activities to find out raw material and environmentally and economically process have been conducted in many countries especially after the oil crisis in early 1970s. Based on raw material processed, bioethanol is grouped into first, second, third and fourth generations. The first generation is derived from sugar or starch, the second generation is derived from lignocellulosic biomass, called as cellulosic ethanol. The third generation is produced from algae, called as Ethanol Algae, while the fourth generation is grouped as advanced biofuels.Indonesia, as a tropical country, posseses various kind of starchy plant, lignocellulosic materials, various species of algae, and organic wastes for ethanol production. Local bioethanol industries utilize food materials such as cassava and sugarcane molasse as feedstock, while universities and R&D institutions have conducted researches to produce the second or the third generations bioethanol. The government of Indonesia has planned to increase utilization of bioethanol in bioethanol-gasoline mixture for transportation up to 15 % (E-15) by 2025. In many countries, utilization of bioethanol for transportation vary in a range from E10 to E 85.This review shows technology development at each generations, and indicates challenges for local R&D institutions in order to develop technology for bioethanol production utilizing local biomass. Prospectives research areas in the field are also highlighted.Keywords :bioethanol, sugarcane molasses, generations, technology development, research challenges.
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Amelework, Assefa B., Michael W. Bairu, Obakeng Maema, Sonja L. Venter, and Mark Laing. "Adoption and Promotion of Resilient Crops for Climate Risk Mitigation and Import Substitution: A Case Analysis of Cassava for South African Agriculture." Frontiers in Sustainable Food Systems 5 (April 15, 2021). http://dx.doi.org/10.3389/fsufs.2021.617783.
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Cassava is an important starchy root crop grown globally in tropical and subtropical regions. The ability of cassava to withstand difficult growing conditions and long-term storability underground makes it a resilient crop, contributing to food security. Historically, small-scale farmers have grown cassava as a minor crop in the far north-eastern part of the country. However, there is an initiative to scale up cassava production, with two discrete areas of interest: large-scale production for industrial starch, and expanding its footprint as a food security crop for small-scale farmers, especially in the context of climate change. In this scoping study, production, processing and marketing data for cassava were accessed from the FAO and US Commercial trade databases. Other domestic market and demand analysis case studies were also explored. There is no cassava data available for South Africa. The study indicated that South Africa imports more than 66,000 tons of starch annually, of which 33% is cassava starch, showing the availability of a local market. The potential of cassava for the South African economy is discussed. Significant industrial opportunities exist for the production and use of cassava in South Africa. However, the realization of these opportunities will depend on the reliable supply of good quality cassava roots. However, the lack of a well-established cassava research program, and a lack of an existing value chain for the industrial scale cassava production and processing are barriers to the development of cassava industry in South Africa. As the initial step to the development of a successful cassava industry, high potential germplasm is imported, characterized and bred for local conditions to ensure the sustainable primary production of cassava. Subsequently, industrial value chains will need to be developed as the optimization of the breeding and agronomy of the crop are completed, and yield potentials are quantified in the different regions of the country.
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Tuo, Decai, Peng Zhou, Pu Yan, Hongguang Cui, Yang Liu, He Wang, Xiukun Yang, et al. "A cassava common mosaic virus vector for virus-induced gene silencing in cassava." Plant Methods 17, no. 1 (July 12, 2021). http://dx.doi.org/10.1186/s13007-021-00775-w.
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Abstract Background Cassava is an important crop for food security and industry in the least-developed and developing countries. The completion of the cassava genome sequence and identification of large numbers of candidate genes by next-generation sequencing provide extensive resources for cassava molecular breeding and increase the need for rapid and efficient gene function analysis systems in cassava. Several plant virus-induced gene silencing (VIGS) systems have been developed as reverse genetic tools for rapid gene function analysis in cassava. However, these VIGS vectors could cause severe viral symptoms or inefficient gene silencing. Results In this study, we constructed agroinfection-compatible infectious cDNA clones of cassava common mosaic virus isolate CM (CsCMV-CM, genus Potexvirus, family Alphaflexiviridae) that causes systemic infection with mild symptoms in cassava. CsCMV-CM was then modified to a viral vector carrying the Nimble cloning frame, which facilitates the rapid and high-throughput cloning of silencing fragments into the viral genome. The CsCMV-based vector successfully silenced phytoene desaturase (PDS) and magnesium chelatase subunit I (ChlI) in different cassava varieties and Nicotiana benthamiana. The silencing of the ChlI gene could persist for more than two months. Conclusions This CsCMV-based VIGS system provides a new tool for rapid and efficient gene function studies in cassava.
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Manze, Francis, Patrick Rubaihayo, Alfred Ozimati, Paul Gibson, Williams Esuma, Anton Bua, Titus Alicai, Chris Omongo, and Robert S. Kawuki. "Genetic Gains for Yield and Virus Disease Resistance of Cassava Varieties Developed Over the Last Eight Decades in Uganda." Frontiers in Plant Science 12 (June 21, 2021). http://dx.doi.org/10.3389/fpls.2021.651992.
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Achieving food security for an ever-increasing human population requires faster development of improved varieties. To this end, assessment of genetic gain for key traits is important to inform breeding processes. Despite the improvements made to increase production and productivity of cassava in Uganda at research level, there has been limited effort to quantify associated genetic gains. Accordingly, a study was conducted in Uganda to assess whether or not genetic improvement was evident in selected cassava traits using cassava varieties that were released from 1940 to 2019. Thirty-two varieties developed during this period, were evaluated simultaneously in three major cassava production zones; central (Namulonge), eastern (Serere), and northern (Loro). Best linear unbiased predictors (BLUPs) of the genotypic value for each clone were obtained across environments and regressed on order of release year to estimate annual genetic gains. We observed that genetic trends were mostly quadratic. On average, cassava mosaic disease (CMD) resistance increased by 1.9% per year, while annual genetic improvements in harvest index (0.0%) and fresh root yield (−5 kg per ha or −0.03% per ha) were non-substantial. For cassava brown streak disease (CBSD) resistance breeding which was only initiated in 2003, average annual genetic gains for CBSD foliar and CBSD root necrosis resistances were 2.3% and 1.5%, respectively. It’s evident that cassava breeding has largely focused on protecting yield against diseases. This underpins the need for simultaneous improvement of cassava for disease resistance and high yield for the crop to meet its current and futuristic demands for food and industry.
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Elegba, Wilfred, Emily McCallum, Wilhelm Gruissem, and Hervé Vanderschuren. "Efficient Genetic Transformation and Regeneration of a Farmer-Preferred Cassava Cultivar From Ghana." Frontiers in Plant Science 12 (May 25, 2021). http://dx.doi.org/10.3389/fpls.2021.668042.
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Cassava is an important staple crop that provides food and income for about 700 million Africans. Cassava productivity in Africa is limited by viral diseases, mainly cassava mosaic disease (CMD) and cassava brown streak disease (CBSD). Genetic barriers such as high heterozygosity, allopolyploidy, poor seed set, and irregular flowering constrain the development of virus-resistant cassava varieties via conventional breeding. Genetic transformation represents a valuable tool to circumvent several challenges associated with the development of virus resistance and other valuable agronomic traits in cassava. The implementation of genetic transformation in many local African cultivars is limited either by the difficulty to produce friable embryogenic callus (FEC), low transformation, and/or regeneration efficiencies. Here, we report the successful induction of organized embryogenic structures (OES) in 11 farmer-preferred cultivars locally grown in Ghana. The production of high quality FEC from one local cultivar, ADI 001, facilitated its genetic transformation with high shoot regeneration and selection efficiency, comparable to the model cassava cultivar 60444. We show that using flow cytometry for analysis of nuclear ploidy in FEC tissues prior to genetic transformation ensures the selection of genetically uniform FEC tissue for transformation. The high percentage of single insertion events in transgenic lines indicates the suitability of the ADI 001 cultivar for the introduction of virus resistance and other useful agronomic traits into the farmer-preferred cassava germplasm in Ghana and Africa.
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Ruiz-Vera, Ursula M., Amanda P. De Souza, Michael R. Ament, Roslyn M. Gleadow, and Donald R. Ort. "High sink strength prevents photosynthetic down-regulation in cassava grown at elevated CO2 concentration." Journal of Experimental Botany, October 12, 2020. http://dx.doi.org/10.1093/jxb/eraa459.
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Abstract Cassava has the potential to alleviate food insecurity in many tropical regions, yet few breeding efforts to increase yield have been made. Improved photosynthetic efficiency in cassava has the potential to increase yields, but cassava roots must have sufficient sink strength to prevent carbohydrates from accumulating in leaf tissue and suppressing photosynthesis. Here, we grew eight farmer-preferred African cassava cultivars under free-air CO2 enrichment (FACE) to evaluate the sink strength of cassava roots when photosynthesis increases due to elevated CO2 concentrations ([CO2]). Relative to the ambient treatments, elevated [CO2] treatments increased fresh (+27%) and dry (+37%) root biomass, which was driven by an increase in photosynthesis (+31%) and the absence of photosynthetic down-regulation over the growing season. Moreover, intrinsic water use efficiency improved under elevated [CO2] conditions, while leaf protein content and leaf and root cyanide concentrations were not affected. Overall, these results suggest that higher cassava yields can be expected as atmospheric [CO2] increases over the coming decades. However, there were cultivar differences in the partitioning of resources to roots versus above-grown biomass; thus, the particular responses of each cultivar must be considered when selecting candidates for improvement.
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Chen, Xia, Hanggui Lai, Ruimei Li, Yuan Yao, Jiao Liu, Shuai Yuan, Shaoping Fu, Xinwen Hu, and Jianchun Guo. "Character changes and Transcriptomic analysis of a cassava sexual Tetraploid." BMC Plant Biology 21, no. 1 (April 19, 2021). http://dx.doi.org/10.1186/s12870-021-02963-1.
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Abstract Background Cassava (Manihot esculenta Crantz) is an important food crop known for its high starch content. Polyploid breeding is effective in its genetic improvement, and use of 2n gametes in sexual polyploid breeding is one of the potential methods for cassava breeding and improvement. In our study, the cassava sexual tetraploid (ST), which carries numerous valuable traits, was successfully generated by hybridizing 2n female gametes SC5 (♀) and 2n male gametes SC10 (♂). However, the molecular mechanisms remain unclear. To understand these underlying molecular mechanisms behind the phenotypic alterations and heterosis in ST plants, we investigated the differences in gene expression between polyploids and diploids by determining the transcriptomes of the ST plant and its parents during the tuber root enlargement period. We also compared the characters and transcriptomes of the ST plant with its parents. Results The ST plant was superior in plant height, stem diameter, leaf area, petiole length, plant weight, and root weight than the parent plants, except the leaf number, which was lower. The number of starch granules was higher in the roots of ST plants than those in the parent plants after five months (tuber root enlargement period), which could be due to a higher leaf net photosynthetic rate leading to early filling of starch granules. Based on transcriptome analysis, we identified 2934 and 3171 differentially expressed genes (DEGs) in the ST plant as compared to its female and male parents, respectively. Pathway enrichment analyses revealed that flavonoid biosynthesis and glycolysis/gluconeogenesis were significantly enriched in the ST plants, which might contribute to the colors of petiole (purple-red), root epidermis (dark brown), and tuber starch accumulation, respectively. Conclusions After sexual polyploidization, the phenotype of ST has changed significantly in comparison to their diploid parents, mainly manifest as enlarged biomass, yield, early starch filling, deep colored petiole and root epidermis. The tetraploid plants were also mature early due to early starch grain filling. Owing to enriched flavonoid biosynthesis and glycolysis/gluconeogenesis, they are possibly resistant to adversity stresses and provide better yield, respectively.
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Wang, Shujuan, Cheng Lu, Xin Chen, Haiyan Wang, and Wenquan Wang. "Comparative Transcriptome Profiling Indicated that Leaf Mesophyll and Leaf Vasculature have Different Drought Response Mechanisms in Cassava." Tropical Plant Biology, September 23, 2021. http://dx.doi.org/10.1007/s12042-021-09302-6.
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AbstractDrought stress is one of the major environmental factors that limited crop’s growth and production. Cassava known as a tropical crop that is widely distributed in Sub-Saharan Africa. It has a strong drought tolerance and can grow well under tough environmental conditions. Therefore, understanding how cassava responds to drought stress and coordinates survival and accumulation has great theoretical significance for improving crop drought resistance breeding. Many studies on cassava drought responses mainly focused on the leaf and whole seedling. Nevertheless, how the vasculature plays an important role in plant response to water deficiency remains to be fully elucidated. Here, comparative transcriptome analysis was performed on isolated mesophyll tissue and leaf vein vascular tissue of cassava variety KU50 after mild drought treatment to determine the molecular mechanism behind drought resistance in cassava vasculature. Our results showed that KU50 leaves had increased leaf temperature, with characters of rapidly decreased net photosynthetic rate, stomatal conductance, and transpiration rate in leaves, and the intercellular CO2 concentration accumulated under drought stress. Comparative transcriptome profiling revealed that under drought stress, leaf mesophyll tissue mainly stimulated the biosynthesis of amino acids, glutamic acid metabolism, and starch and sucrose metabolism. In particular, the arginine biosynthesis pathway was significantly enhanced to adapt to the water deficiency in leaf mesophyll tissue. However, in vascular tissue, the response to drought mainly involved ion transmembrane transport, hormone signal transduction, and depolymerization of proteasome. Concretely, ABA signaling and proteasome metabolism, which are involved in ubiquitin regulation, were changed under drought stress in KU50 leaf vascular tissue. Our work highlights that the leaf vasculature and mesophyll in cassava have completely different drought response mechanisms.
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S, Sharmiladevi, Ramesh N, and Ramesh S. "Production of Bio Degradable Bags Using cassava Starch." International Research Journal of Multidisciplinary Technovation, November 3, 2019, 553–59. http://dx.doi.org/10.34256/irjmtcon80.
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Plastic is a material that is used to a great extent. Nowadays Petroleum based plastics are widely used for the commercial purpose. This kind of plastics can take more than a century to degrade. Man made plastics have Polyurethane and Polyethylene polymers. It cannot be easily degraded by micro organisms as it don’t recognize as food. When burned plastics release cancer causing carcinogenic chemicals that are harmful to people and the environment. One eco friendly alternative the current commercial plastic is “Bio plastics”. Bio plastics are biodegradable plastics that have components derived directly from renewable raw plant materials. Seven type of bioplastics were made using various combinations of renewable raw materials and plasticizers. In this project, Cassava starch is used as a main biopolymer for the production of bio degradable bags.
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Navangi, L. N., S. M. Githiri, E. M. Ateka, E. Kanju, S. Tumwegamire, and T. L. Munga. "Influence of Genotype, Location and Plant Age on Incidence and Severity of Cassava Mosaic and Cassava Brown Streak Diseases in Western Kenya." Journal of Experimental Agriculture International, July 22, 2020, 86–104. http://dx.doi.org/10.9734/jeai/2020/v42i630543.
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This study aimed to assess elite cassava genotypes for resistance to cassava mosaic and brown streak diseases at Alupe, Kakamega and Kibos in Western Kenya. The trial was conducted using alpha lattice balanced design using 24 genotypes with three replicates, for an extended cropping cycle between 2016 and 2017. Results for combined analysis of variance showed that genotype, location, month after planting (MAP) and their interactions significantly influenced (P ≤ 0.05) incidence and severity of CMD and CBSD. High CMD incidence and severity was recorded across all cassava genotypes at Alupe (mean 0.730; 1.256) as opposed to Kakamega (mean 0.000; 1.000) and Kibos (mean 0.031; 1.006). Similarly, CBSD root incidence and severity were high in Alupe (mean 0.848; 1.310), as opposed to Kakamega (mean 0.020; 1.006) and Kibos (mean 0.188; 1.078). Within location analysis for CMD and CBSD incidence and severity among cassava genotypes 12 MAP gave varied results. Genotypes Kibandameno and Kalawe had the highest CMD and CBSD incidence and severity in all three locations. Whiteflies abundance was significantly influenced (P ≤ 0.05) by genotype, location, MAP time and interaction. Significant interaction (P ≤ 0.05) between all disease resistance traits further confirmed dual resistance amongst the cassava genotypes, however, this was location specific and not generalized. These findings should be of value to cassava breeding and development efforts throughout Kenya, and other parts of sub Saharan Africa affected or threatened by CMD and CBSD and will hopefully contribute to the development of much improved and/or resistant genotypes and, ultimately more effective management of two of Africa's most pernicious threats to food security
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Edmond, Koffi Kouablan, Yao Saraka Didier Martial, Kouakou Amani Michel, N’Zue Boni, Kobenan Kouman, Traore Siaka, Anguete Kouamé, and Diarrassouba Nafan. "Systems of Genetic Resources Exchange in Côte D’Ivoire and its Evolution: Case Study of Food Crops Such as Yam, Cassava, Rice and Plantain." Journal of Agriculture and Ecology Research International, May 22, 2021, 1–16. http://dx.doi.org/10.9734/jaeri/2021/v22i330188.
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Aims: Plant Genetic Resources for Food and Agriculture (PGRFA) flow in research and development area inside and outside many countries are not indexed or listed and are insufficiently documented. In Côte d’Ivoire, when implementing the Multilateral System (MLS) project, a survey was realized with different actors as well at national as international levels to collect data dealing with exchanges flow characteristics of main food crops as yam, cassava, rice and plantain. The objectives of the current study are i) to know about internal and external exchange flow of genetic resources of yam, cassava, rice and plantain in Côte d’Ivoire, ii) to assess Côte d’Ivoire dependence regarding external PGRFA, iii) to indentify international research agreements or research network facilitating Plant Genetic Resources (PGR) in Côte d’Ivoire and iv) to determine constraints or opportunities related to access to external PGR in the future.
Study Design: A questionnaire was submitted to actors managing main consumed food crops in Côte d’Ivoire such as yam, cassava, rice and plantain.
Place and Duration of Study: Study was conducted in 2019 and sampled data on period starting from 2005 to 2014 about the flow of genetic resources of yam, cassava, rice and plantain inside and outside Côte d’Ivoire located in Western Africa were studied.
Methodology: This comprised the collection of data using a questionnaire related to characteristics of PGRFA flow inside and outside Côte d’Ivoire, within institutions in charge of PGRFA. A survey was conducted with national and international actors involved in yam, cassava, rice and plantain genetic resources. Moreover, surveys on PGRFA flow data were supplemented by reports from research programs dedicated to these food crops.
Results: Results revealed that genetic resources of yam, cassava, rice and plantain are exchanged at variable levels within different actors. Contrary to plantain, a growth of vegetal material exchange within actors was observed at national level in yam, cassava and rice. PGRFA exchange flow at international level are positively unbalanced in favor of importation from Groupe Consultatif pour la Recherche Agricole Internationale (CGIAR) creating by this way a relatively marked dependence excepted to yam where ongoing varietal breeding relies on local genetic resources. Very few or no data was given back to national research and development institutions and farmers from Côte d’Ivoire on national PGRFA sent outside for breeding purposes. In the same line, neither any law nor regulations were set up at national level to ensure traceability of plant material exchanges according to international agreements and conventions.
Conclusion: The consequence of this inventory of fixtures about systems of genetic resources exchange and evolution in Côte d’Ivoire is the lack of regulatory mechanisms allowing populations owning traditional knowledge take profit of an access and a fair sharing of advantages arising from the use of PGRFA as yam, cassava, rice and plantain.
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Perez-Fons, Laura, Adriana Bohorquez-Chaux, Maria L. Irigoyen, Danielle C. Garceau, Kris Morreel, Wout Boerjan, Linda L. Walling, Luis Augusto Becerra Lopez-Lavalle, and Paul D. Fraser. "A metabolomics characterisation of natural variation in the resistance of cassava to whitefly." BMC Plant Biology 19, no. 1 (November 27, 2019). http://dx.doi.org/10.1186/s12870-019-2107-1.
Full textAbstract:
Abstract Background Cassava whitefly outbreaks were initially reported in East and Central Africa cassava (Manihot esculenta Crantz) growing regions in the 1990’s and have now spread to other geographical locations, becoming a global pest severely affecting farmers and smallholder income. Whiteflies impact plant yield via feeding and vectoring cassava mosaic and brown streak viruses, making roots unsuitable for food or trading. Deployment of virus resistant varieties has had little impact on whitefly populations and therefore development of whitefly resistant varieties is also necessary as part of integrated pest management strategies. Suitable sources of whitefly resistance exist in germplasm collections that require further characterization to facilitate and assist breeding programs. Results In the present work, a hierarchical metabolomics approach has been employed to investigate the underlying biochemical mechanisms associated with whitefly resistance by comparing two naturally occurring accessions of cassava, one susceptible and one resistant to whitefly. Quantitative differences between genotypes detected at pre-infestation stages were consistently observed at each time point throughout the course of the whitefly infestation. This prevalent differential feature suggests that inherent genotypic differences override the response induced by the presence of whitefly and that they are directly linked with the phenotype observed. The most significant quantitative changes relating to whitefly susceptibility were linked to the phenylpropanoid super-pathway and its linked sub-pathways: monolignol, flavonoid and lignan biosynthesis. These findings suggest that the lignification process in the susceptible variety is less active, as the susceptible accession deposits less lignin and accumulates monolignol intermediates and derivatives thereof, differences that are maintained during the time-course of the infestation. Conclusions Resistance mechanism associated to the cassava whitefly-resistant accession ECU72 is an antixenosis strategy based on reinforcement of cell walls. Both resistant and susceptible accessions respond differently to whitefly attack at biochemical level, but the inherent metabolic differences are directly linked to the resistance phenotype rather than an induced response in the plant.