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Journal articles on the topic 'Cassava mosaic virus'

Author: Grafiati

Published: 4 June 2021

Last updated: 26 July 2025

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1

RAJU, J., P. R. Thimmegowda, C. Elangovan, Sunita Pandey, and J. P. Singh. "Occurrence, Characterization and management of Sri Lankan Cassava Mosaic Virus (SLCMV) in cassava growing Namakkal district of Tamil Nadu, India." JOURNAL OF ECO-FRIENDLY AGRICULTURE 18, no. 2 (2023): 377–81. http://dx.doi.org/10.48165/jefa.2023.18.02.29.

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Cassava mosaic disease caused by begomoviruses of Geminiviridae family is one of the major constraints for the Cassava production in many Cassava growing states of India. The disease is known to be transmitted by whiteflies Bemisia tabaci and spread though the infected Cassava planting material. In India the Cassava mosaic disease (CMD) is caused by both Indian cassava mosaic virus (ICMV) and Sri Lankan cassava mosaic virus (SLCMV). However, Sri Lankan cassava mosaic virus was the most predominant virus species in the study area. To assess the severity of CMD, a survey was conducted during 202

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2

Sserubombwe, W. S., R. W. Briddon, Y. K. Baguma, et al. "Diversity of begomoviruses associated with mosaic disease of cultivated cassava (Manihot esculenta Crantz) and its wild relative (Manihot glaziovii Müll. Arg.) in Uganda." Journal of General Virology 89, no. 7 (2008): 1759–69. http://dx.doi.org/10.1099/vir.0.83637-0.

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Cassava (Manihot esculenta) growing in Uganda during 2001–2002 has been screened for the presence of begomoviruses using PCR-RFLP, cloning full-length genomic components and nucleotide sequence analysis. In contrast with a recent survey in neighbouring Kenya, which identified three distinct strains of East African cassava mosaic virus (EACMV, EACMV-UG and EACMV-KE2) as well as East African cassava mosaic Zanzibar virus and the new species East African cassava mosaic Kenya virus, only EACMV-UG and, to a lesser extent, African cassava mosaic virus (ACMV) were found associated with cassava in Uga

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3

Shirima, Rudolph Rufini, Everlyne Nafula Wosula, Abdou Azali Hamza, et al. "Epidemiological Analysis of Cassava Mosaic and Brown Streak Diseases, and Bemisia tabaci in the Comoros Islands." Viruses 14, no. 10 (2022): 2165. http://dx.doi.org/10.3390/v14102165.

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A comprehensive assessment of cassava brown streak disease (CBSD) and cassava mosaic disease (CMD) was carried out in Comoros where cassava yield (5.7 t/ha) is significantly below the African average (8.6 t/ha) largely due to virus diseases. Observations from 66 sites across the Comoros Islands of Mwali, Ngazidja, and Ndzwani revealed that 83.3% of cassava fields had foliar symptoms of CBSD compared with 95.5% for CMD. Molecular diagnostics confirmed the presence of both cassava brown streak ipomoviruses (CBSIs) and cassava mosaic begomoviruses (CMBs). Although real-time RT-PCR only detected t

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4

Mulenga, Rabson M., James P. Legg, Joseph Ndunguru, et al. "Survey, Molecular Detection, and Characterization of Geminiviruses Associated with Cassava Mosaic Disease in Zambia." Plant Disease 100, no. 7 (2016): 1379–87. http://dx.doi.org/10.1094/pdis-10-15-1170-re.

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A survey was conducted from April to May 2014 in 214 farmers’ fields located across six major cassava-producing provinces (Western, Northwestern, Northern, Luapula, Lusaka, and Eastern) of Zambia to determine the status of cassava mosaic disease (CMD) and the species diversity of associated cassava mosaic geminiviruses (CMG). Mean CMD incidence varied across all six provinces but was greatest in Lusaka Province (81%) and least in Northern Province (44%). Mean CMD severity varied slightly between provinces, ranging from 2.78 in Eastern Province to 3.00 in Northwestern Province. Polymerase chain

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5

Ogbe, F. O., G. Thottappilly, A. G. O. Dixon, G. I. Atiri, and H. D. Mignouna. "Variants of East African cassava mosaic virus and Its Distribution in Double Infections with African cassava mosaic virus in Nigeria." Plant Disease 87, no. 3 (2003): 229–32. http://dx.doi.org/10.1094/pdis.2003.87.3.229.

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In a survey for cassava mosaic begomoviruses conducted in 1997 and 1998 in Nigeria, East African cassava mosaic virus (EACMV) was detected by the polymerase chain reaction together with African cassava mosaic virus (ACMV) in 27 out of 290 cassava leaf samples of infected plants from 254 farmers' fields in five agroecological zones. One plant was infected with EACMV only. Five variant isolates of EACMV were observed based on their reactions to primers that could detect Cameroonian and East African strains of EACMV. Isolates of variants 1 and 3 occurred mostly in the derived or coastal and south

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6

Ogbe, F. O., A. G. O. Dixon, J. d'A Hughes, O. J. Alabi, and R. Okechukwu. "Status of Cassava Begomoviruses and Their New Natural Hosts in Nigeria." Plant Disease 90, no. 5 (2006): 548–53. http://dx.doi.org/10.1094/pd-90-0548.

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A diagnostic survey was conducted in 2002-03 to determine the status of cassava mosaic begomoviruses in Nigeria and to ascertain if the virulent Ugandan variant of East African cassava mosaic virus (EACMV-Ug2) was present. Of the 418 farms visited, 48% had cassava with moderately severe or severe symptoms, whereas 52% had cassava with mild symptoms. These distributions were at random. Of the 1,397 cassava leaf samples examined, 1,106 had symptoms. In polymerase chain reaction tests, 74.1% of the symptom-bearing samples tested positive for African cassava mosaic virus (ACMV) alone, 0.3% for EAC

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7

Bull, Simon E., Rob W. Briddon, William S. Sserubombwe, Kahiu Ngugi, Peter G. Markham, and John Stanley. "Genetic diversity and phylogeography of cassava mosaic viruses in Kenya." Journal of General Virology 87, no. 10 (2006): 3053–65. http://dx.doi.org/10.1099/vir.0.82013-0.

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Cassava is a major factor in food security across sub-Saharan Africa. However, the crop is susceptible to losses due to biotic stresses, in particular to viruses of the genus Begomovirus (family Geminiviridae) that cause cassava mosaic disease (CMD). During the 1990s, an epidemic of CMD severely hindered cassava production across eastern and central Africa. A significant influence on the appearance of virus epidemics is virus diversity. Here, a survey of the genetic diversity of CMD-associated begomoviruses across the major cassava-growing areas of Kenya is described. Because an initial PCR-re

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8

Vanitharani, Ramachandran, Padmanabhan Chellappan, Justin S. Pita, and Claude M. Fauquet. "Differential Roles of AC2 and AC4 of Cassava Geminiviruses in Mediating Synergism and Suppression of Posttranscriptional Gene Silencing." Journal of Virology 78, no. 17 (2004): 9487–98. http://dx.doi.org/10.1128/jvi.78.17.9487-9498.2004.

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ABSTRACT Posttranscriptional gene silencing (PTGS) in plants is a natural defense mechanism against virus infection. In mixed infections, virus synergism is proposed to result from suppression of the host defense mechanism by the viruses. Synergistic severe mosaic disease caused by simultaneous infection with isolates of the Cameroon strain of African cassava mosaic virus (ACMV-[CM]) and East African cassava mosaic Cameroon virus (EACMCV) in cassava and tobacco is characterized by a dramatic increase in symptom severity and a severalfold increase in viral-DNA accumulation by both viruses compa

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9

Bull, Simon E., Rob W. Briddon, William S. Sserubombwe, Kahiu Ngugi, Peter G. Markham, and John Stanley. "Infectivity, pseudorecombination and mutagenesis of Kenyan cassava mosaic begomoviruses." Journal of General Virology 88, no. 5 (2007): 1624–33. http://dx.doi.org/10.1099/vir.0.82662-0.

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Cloned DNA-A and DNA-B components of Kenyan isolates of East African cassava mosaic virus (EACMV, EACMV-UG and EACMV-KE2), East African cassava mosaic Kenya virus (EACMKV) and East African cassava mosaic Zanzibar virus (EACMZV) are shown to be infectious in cassava. EACMV and EACMKV genomic components have the same iteron sequence (GGGGG) and can form viable pseudorecombinants, while EACMZV components have a different sequence (GGAGA) and are incompatible with EACMV and EACMKV. Mutagenesis of EACMZV has demonstrated that open reading frames (ORFs) AV1 (encoding the coat protein), AV2 and AC4 a

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10

Efekemo, Oghenevwairhe P., Olabode A. Onile-ere, Isaac O. Abegunde, et al. "Molecular Diversity and Distribution of Whiteflies (Bemisia tabaci) in Cassava Fields Across South West and North Central, Nigeria." Insects 15, no. 11 (2024): 906. http://dx.doi.org/10.3390/insects15110906.

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Whitefly Bemisia tabaci (Gennadium, Hemiptera) causes severe damage to cassava plants through excessive feeding on leaves and transmitting viruses, such as African cassava mosaic virus (ACMV), East African cassava mosaic virus (EACMV), and ipomoviruses that cause cassava brown streak disease. Currently, little is known about the molecular diversity and distribution of whitefly species in the major cassava-growing zones of Nigeria. This study aimed to address the knowledge gap by assessing the genetic diversity, distribution, and associated cassava mosaic begomoviruses (CMBs) in whiteflies acro

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11

Alabi, Olufemi J., P. Lava Kumar, and Rayapati A. Naidu. "Multiplex PCR for the detection of African cassava mosaic virus and East African cassava mosaic Cameroon virus in cassava." Journal of Virological Methods 154, no. 1-2 (2008): 111–20. http://dx.doi.org/10.1016/j.jviromet.2008.08.008.

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12

Mwakosya, Joseph A., Gladness E. Temu, and Joseph C. Ndunguru. "Identification and Characterization of Cassava Mosaic Begomoviruses in Non-crop Plants from Unguja and Pemba Islands." Tanzania Journal of Science 47, no. 5 (2022): 1870–81. http://dx.doi.org/10.4314/tjs.v47i5.31.

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Cassava mosaic disease caused by cassava mosaic begomoviruses (CMBs) remains one of the major constraints to cassava production since it causes yield losses up to 100%. Researches showed that some of CMBs infecting cassava plants may be coming from non-crop plants. This study intended to identify and characterize CMBs isolated from non-crop plant species in Unguja and Pemba Islands. A total of 108 viral symptomatic and asymptomatic non-crop leaf samples were collected using stratified sampling. DNA was extracted by using CTAB protocol followed by polymerase chain reaction using universal and s

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13

Kittelmann, Katharina, and Holger Jeske. "Disassembly of African cassava mosaic virus." Journal of General Virology 89, no. 8 (2008): 2029–36. http://dx.doi.org/10.1099/vir.0.2008/000687-0.

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The plant-infecting geminiviruses encapsidate their single-stranded DNA genome in characteristic twinned particles that are unique among viruses. These particles are formed by joining two incomplete T=1 icosahedra. African cassava mosaic virions were purified by density-gradient centrifugation from infected Nicotiana benthamiana plants and analysed for their stability with respect to pH changes and heat treatment by using electron microscopy. Negative staining and rotary shadowing revealed stable virions as well as isolated capsomeres between pH 4.0 and 8.5. At pH 9.0 and above, particles disi

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14

Alabi, Olufemi J., Francis O. Ogbe, Ranajit Bandyopadhyay, et al. "Alternate hosts of African cassava mosaic virus and East African cassava mosaic Cameroon virus in Nigeria." Archives of Virology 153, no. 9 (2008): 1743–47. http://dx.doi.org/10.1007/s00705-008-0169-8.

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15

Annang, Esther Afoley, Allen Oppong, Ruth N. A. Prempeh, et al. "Using PCR and RCA Techniques to Investigate the Variants of Cassava Mosaic Virus and Their Distribution in Ghana." Journal of Agricultural Science 16, no. 7 (2024): 98. http://dx.doi.org/10.5539/jas.v16n7p98.

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Cassava mosaic disease (CMD) caused by cassava begomoviruses is the major constraint to cassava production in Ghana. The disease is known to cause reduction in root yield. To ascertain the distribution of viruses causing CMD, 95 diseased cassava samples were collected in two agroecological zones of Ghana-Deciduous Forest zone and the Transitional zone. On a scale of 1-5, CMD severity was scored. Mean CMD severity score was 2.9, however there was no significant difference (p &gt; 0.05) between the zones. Averagely, CMD score of &gt; 2.8 in 71% of farms visited was recorded. Polymerase c

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16

Combala, Mariam, Justin S. Pita, Michel Gbonamou, et al. "An Alarming Eastward Front of Cassava Mosaic Disease in Development in West Africa." Viruses 16, no. 11 (2024): 1691. http://dx.doi.org/10.3390/v16111691.

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Begomoviruses are a major threat to cassava production in Africa. Indeed, during the 1990s, the emergence of a recombinant begomovirus (East African cassava mosaic virus-Uganda, EACMV-Ug) resulted in crop devastation and severe famine in Uganda. In 2023, during a pre-survey of cassava farms at Forécariah, South-West Guinea, 22 samples showing peculiar cassava mosaic disease (CMD) symptoms were collected, and subsequent laboratory analysis confirmed the presence of EACMV-Ug in the samples. Deep analysis of DNA-A and DNA-B of the EACMV-Ug isolates from Guinea indicated that they are similar to t

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17

FARGETTE, D., J.-C. THOUVENEL, and C. FAUQUET. "Virus content of leaves of cassava infected by African cassava mosaic virus." Annals of Applied Biology 110, no. 1 (1987): 65–73. http://dx.doi.org/10.1111/j.1744-7348.1987.tb03233.x.

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18

Offei, S. K., M. Owuna-Kwakye, and G. Thottappilly. "First Report of East African Cassava Mosaic Begomovirus in Ghana." Plant Disease 83, no. 9 (1999): 877. http://dx.doi.org/10.1094/pdis.1999.83.9.877c.

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Virus species causing cassava mosaic disease have been categorized into three classes based on their reaction with monoclonal antibodies (MAbs) and their distribution (2). These viruses have different, scarcely overlapping distribution: African cassava mosaic begomovirus (ACMV) occurs in Africa west of the Rift Valley and in South Africa; East African cassava mosaic (EACMV) occurs in Africa east of the Rift Valley and in Madagascar; and Indian cassava mosaic virus (ICMV) occurs in India and Sri Lanka (2). During 1998, surveys were conducted in farmers' fields in Ghana to assess the incidence a

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19

Allado, Senya Sakina, Kossikouma Djodji Adjata, Justin Simon Pita, Kodjovi Atassé Dansou-Kodjo, Assion Sétu Mivedor, and Koffi Tozo. "East African Cassava Mosaic Virus and East African Cassava Mosaic Cameroon Virus: Two Species Emerging in Togo." Agricultural Sciences 15, no. 08 (2024): 864–76. http://dx.doi.org/10.4236/as.2024.158048.

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20

Pita, J. S., V. N. Fondong, A. Sangaré, G. W. Otim-Nape, S. Ogwal, and C. M. Fauquet. "Recombination, pseudorecombination and synergism of geminiviruses are determinant keys to the epidemic of severe cassava mosaic disease in Uganda." Journal of General Virology 82, no. 3 (2001): 655–65. http://dx.doi.org/10.1099/0022-1317-82-3-655.

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The molecular variability of cassava geminiviruses occurring in Uganda was investigated in this study. Infected cassava plants and whiteflies were collected from cassava plantings in different geographical areas of the country and PCR was used for molecular characterization of the viruses. Two complete sequences of DNA-A and -B from African cassava mosaic virus (ACMV), two DNA-A sequences from East African cassava mosaic virus (EACMV), two DNA-B sequences of EACMV and the partial DNA-A nucleotide sequence of a new virus strain isolated in Uganda, EACMV-UG3, are reported here. Analysis of natur

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21

Chellappan, Padmanabhan, Ramachandran Vanitharani, and Claude M. Fauquet. "Short Interfering RNA Accumulation Correlates with Host Recovery in DNA Virus-Infected Hosts, and Gene Silencing Targets Specific Viral Sequences." Journal of Virology 78, no. 14 (2004): 7465–77. http://dx.doi.org/10.1128/jvi.78.14.7465-7477.2004.

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ABSTRACT Viruses are both inducers and targets of posttranscriptional gene silencing (PTGS), a natural defense mechanism in plants. Here we report molecular evidence of the ability of single-stranded DNA (ssDNA) viruses to induce PTGS in infected plants irrespective of the severity of or recovery from the symptoms. Our results reveal that five distinct species of cassava-infecting geminiviruses were capable of triggering PTGS by producing two classes of virus-specific short interfering RNAs (siRNAs) of 21 to 26 nucleotides in two plant hosts, tobacco (Nicotiana benthamiana) and cassava (Maniho

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22

Ekpiken, E. E., O. I. Eyong, and A. O. Adediji. "Occurrence and molecular characterization of begomoviruses associated with cassava across agro-ecological zones in Cross River State, Nigeria." Journal of Applied Horticulture 24, no. 01 (2022): 9–15. http://dx.doi.org/10.37855/jah.2022.v24i01.02.

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Cassava (Manihot esculenta Crantz) is a major root crop in sub-Saharan Africa, including Cross River State, Nigeria, where the crop is cultivated as a staple food in many communities. Cassava mosaic begomoviruses (CMBs) have limited cassava production across Africa. Thus, between March and August 2021, forty-five samples of symptomatic cassava leaves across the state were collected and evaluated for CMBs using molecular techniques. Partial sequencing of the African cassava mosaic virus (ACMV) and East African cassava mosaic virus (EACMV) coat protein genes produced sequence lengths of 620 bp f

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23

GIBSON, R. W., and G. W. OTIM-NAPE. "Factors determining recovery and reversion in mosaic-affected African cassava mosaic virus resistant cassava." Annals of Applied Biology 131, no. 2 (1997): 259–71. http://dx.doi.org/10.1111/j.1744-7348.1997.tb05155.x.

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24

Fondong, V. N., J. S. Pita, M. E. C. Rey, A. de Kochko, R. N. Beachy, and C. M. Fauquet. "Evidence of synergism between African cassava mosaic virus and a new double-recombinant geminivirus infecting cassava in Cameroon." Microbiology 81, no. 1 (2000): 287–97. http://dx.doi.org/10.1099/0022-1317-81-1-287.

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Stem cuttings were collected in Cameroon from cassava plants displaying cassava mosaic disease (CMD) symptoms. The nature of the viruses present was determined by using the PCR with primers specific for the coat protein (CP) genes of African cassava mosaic virus (ACMV) and East African cassava mosaic virus (EACMV). All samples were infected by ACMV and eight of the 50 samples were infected by both ACMV and an EACMV-like virus. The complete nucleotide sequences of DNA-A and -B of representative ACMV and EACMV-like viruses were determined. The DNA-A component of the EACMV-like virus contained ev

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Lentz, Ezequiel Matias, Joel-Elias Kuon, Adrian Alder, et al. "Cassava geminivirus agroclones for virus-induced gene silencing in cassava leaves and roots." Plant Methods 14, no. 1 (2018): 73. https://doi.org/10.1186/s13007-018-0340-5.

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<strong>Aim: </strong>We report the construction of a Virus-Induced Gene Silencing (VIGS) vector and an agroinoculation protocol for gene silencing in cassava (<i>Manihot esculenta</i> Crantz) leaves and roots. The African cassava mosaic virus isolate from Nigeria (ACMV-[NOg]), which was initially cloned in a binary vector for agroinoculation assays, was modified for application as VIGS vector. The functionality of the VIGS vector was validated in <i>Nicotiana benthamiana</i> and subsequently applied in wild-type and transgenic cassava plants expressing the <i>uidA</i> gene under the control o

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Ndunguru, Joseph, Leandro De León, Catherine D. Doyle, et al. "Two Novel DNAs That Enhance Symptoms and Overcome CMD2 Resistance to Cassava Mosaic Disease." Journal of Virology 90, no. 8 (2016): 4160–73. http://dx.doi.org/10.1128/jvi.02834-15.

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ABSTRACTCassava mosaic begomoviruses (CMBs) cause cassava mosaic disease (CMD) across Africa and the Indian subcontinent. Like all members of the geminivirus family, CMBs have small, circular single-stranded DNA genomes. We report here the discovery of two novel DNA sequences, designated SEGS-1 and SEGS-2 (forsequencesenhancinggeminivirussymptoms), that enhance symptoms and break resistance to CMD. The SEGS are characterized by GC-rich regions and the absence of long open reading frames. Both SEGS enhanced CMD symptoms in cassava (Manihot esculentaCrantz) when coinoculated withAfrican cassava

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Fargette, D., L. T. Colon, R. Bouveau, and C. Fauquet. "Components of resistance of cassava to African cassava mosaic virus." European Journal of Plant Pathology 102, no. 7 (1996): 645–54. http://dx.doi.org/10.1007/bf01877245.

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Saffa, Musa Decius, Alusaine Edward Samura, Mohamed Alieu Bah, et al. "Identification and Distribution of Begomoviruses Infecting Cassava Fields in Sierra Leone." Plants 14, no. 14 (2025): 2142. https://doi.org/10.3390/plants14142142.

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A dearth of knowledge exists on identifying the begomoviruses and distributing cassava mosaic viruses across key cassava-growing regions of Sierra Leone. The study aimed to identify and map the distribution of cassava mosaic disease (CMD)-associated viruses in farmers’ fields in Sierra Leone. Cassava (Manihot esculenta Crantz) leaf samples were collected in 109 smallholder farms during a geo-referenced survey conducted from 10th May to 5th June 2024. Molecular diagnostics were carried out to identify the viral strains associated with CMD. Findings revealed that infection by stem cutting was mo

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Berrie, L. C., E. P. Rybicki, and M. E. C. Rey. "Complete nucleotide sequence and host range of South African cassava mosaic virus: further evidence for recombination amongst begomoviruses." Journal of General Virology 82, no. 1 (2001): 53–58. http://dx.doi.org/10.1099/0022-1317-82-1-53.

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Complete nucleotide sequences of the DNA-A (2800 nt) and DNA-B (2760 nt) components of a novel cassava-infecting begomovirus, South African cassava mosaic virus (SACMV), were determined and compared with various New World and Old World begomoviruses. SACMV is most closely related to East African cassava mosaic virus (EACMV) in both its DNA-A (85% with EACMV-MH and -MK) and -B (90% with EACMV-UG2-Mld and EACMV-UG3-Svr) components; however, percentage sequence similarities of less than 90% in the DNA-A component allowed SACMV to be considered a distinct virus. One significant recombination event

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Ezeji, L. A., A. O. Adediji, C. K. Nkere, O. C. Ogbe, J. T. Onyeka, and G. I. Atiri. "Viruses associated with cassava mosaic disease and their alternative hosts along Nigeria-Cameroon border." African Crop Science Journal 31, no. 3 (2023): 263–77. http://dx.doi.org/10.4314/acsj.v31i3.1.

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Cassava mosaic disease (CMD) threatens cassava production across the African continent. Because CMD is widespread in sub-Saharan Africa (SSA), it is particularly important to monitor border regions to prevent the introduction of other cassava (Manihot esculenta Crantz) viruses and strains into regions otherwise considered still free of the viruses. The objective of this study was to establish the occurrence of viruses associated with CMD and their alternate host plants along the Nigeria-Cameroon border. One hundred leaf samples from cassava plants and weed species were collected across 21 loca

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Colariccio, Addolorata, Eliana Borges Rivas, Mauro Ferreira Bonfim Junior, and Luiz Augusto Martins Peruch. "Ocorrência do Cassava Common Mosaic Vírus (CsCMV) e Cassava Vein Mosaic Vírus (CsVMV) em cultivares de mandioca em Santa Catarina, Brasil." O Biológico 82, no. 1 (2020): 1–9. http://dx.doi.org/10.31368/1980-6221v82a10001.

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O cultivo da mandioca é realizado na maioria dos municípios e estados brasileiros, sendo uma pequena parte destinada ao consumo in natura e a maior parte para a produção de farinha e extração de amido. Embora seja uma planta rústica, diversos fatores bióticos e abióticos podem reduzir a produtividade da cultura. As doenças viróticas causam impacto na cultura, principalmente devido a eficiente disseminação do patógeno por meio da propagação vegetativa das manivas infectadas e degenerescência das raízes. No Brasil já foram relatados o Cassava common mosaic virus (CsCMV), Cassava vein mosaic viru

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Thresh, J. M. "AFRICAN CASSAVA MOSAIC VIRUS: AN UNSOLVED PROBLEM." Acta Horticulturae, no. 380 (November 1994): 517. http://dx.doi.org/10.17660/actahortic.1994.380.96.

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33

Böttcher, Bettina, Sigrid Unseld, Hugo Ceulemans, Robert B. Russell, and Holger Jeske. "Geminate Structures of African Cassava Mosaic Virus." Journal of Virology 78, no. 13 (2004): 6758–65. http://dx.doi.org/10.1128/jvi.78.13.6758-6765.2004.

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ABSTRACT Two types of geminate structures were purified from African cassava mosaic geminivirus (ACMV)-infected Nicotiana benthamiana plants and analyzed by electron cryomicroscopy and image reconstruction. After cesium sulfate density gradient centrifugation, they were separated into lighter top (T) and heavier bottom (B) components. T particles comigrated with host proteins, whereas B particles were concentrated in a cesium density typical for complete virions. Both particles were composed of two incomplete icosahedra of 11 capsomers each, but T particles were slightly larger (diameter, 22.5

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Di Feo, L., A. Zanini, P. Rodríguez Pardina, M. Cuervo, M. Carvajal-Yepes, and W. J. Cuellar. "First Report of Cassava common mosaic virus and Cassava frogskin-associated virus Infecting Cassava in Argentina." Plant Disease 99, no. 5 (2015): 733. http://dx.doi.org/10.1094/pdis-10-14-1088-pdn.

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Kouakou, Bekanvié S. M., Aya Ange Naté Yoboué, Justin S. Pita, et al. "Gradual Emergence of East African cassava mosaic Cameroon virus in Cassava Farms in Côte d’Ivoire." Agronomy 14, no. 3 (2024): 418. http://dx.doi.org/10.3390/agronomy14030418.

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Cassava mosaic disease (CMD) and cassava brown streak disease (CBSD) are endemic threats to cassava production, causing significant yield losses. They are caused respectively by begomoviruses and ipomoviruses that are transmitted by whiteflies and infected cuttings. This study aimed to monitor and characterize viruses responsible for these diseases in order to fill existing gaps in understanding their epidemiology in Côte d’Ivoire. Field surveys were conducted in 2016, 2017, and 2020, and no CBSD symptoms were observed. However, an increase in CMD incidence was noted from 45.95% in 2016 to 51.

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Fernandez, E., I. Espinoza, I. Lozano, C. Bolaños, M. Carvajal-Yepes, and W. J. Cuellar. "First Report of Cassava Common Mosaic Disease and Cassava common mosaic virus Infecting Cassava (Manihot esculenta) in Peru." Plant Disease 101, no. 6 (2017): 1066. http://dx.doi.org/10.1094/pdis-10-16-1540-pdn.

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Badamasi, H., M. D. Alegbejo, B. D. Kashina, and O. O. Banwo. "INCIDENCE AND DISTRIBUTION OF VIRUSES INFECTING CASSAVA IN KADUNA AND SOKOTO STATES, NIGERIA." FUDMA JOURNAL OF SCIENCES 4, no. 2 (2020): 325–36. http://dx.doi.org/10.33003/fjs-2020-0402-221.

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Field survey was conducted in the 2015 wet season to determine the incidence and distribution of cassava viruses in Kaduna and Sokoto States, Nigeria. Eighteen farms from 3 Local Government Areas namely; Lere, Chikun and Kajuru (Kaduna State) and Tureta, Shagari and Tambuwal (Sokoto State) were surveyed. Symptomatic leaves (180) and asymptomatic leaves (90) were collected from the surveyed farms. Enzyme Linked Immosorbent Assay (ELISA) technique was used to test the presence of viruses infecting cassava. Three viruses: African cassava mosaic virus (ACMV), East African cassava mosaic virus (EAC

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Maruthi, M. N., J. Colvin, S. Seal, and J. M. Thresh. "First Report of a Distinct Begomovirus Infecting Cassava from Zanzibar." Plant Disease 86, no. 2 (2002): 187. http://dx.doi.org/10.1094/pdis.2002.86.2.187a.

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In 1998, cassava plants exhibiting extremely mild mosaic disease symptoms were collected from Uguja Island, Zanzibar. Total DNAs extracted from symptomatic leaves did not produce diagnostic PCR bands using primers specific to known Cassava mosaic viruses (genus Begomovirus, family Geminiviridae) (2). Degenerate primer pair A/B (1), however, produced a 564-nucleotide (nt) band in the common region of DNA-A to the conserved amino acid sequence CEGPCKYG within the coat protein gene for begomoviruses. Virus-specific primers were designed and the begomoviral genome was amplified and cloned to obtai

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Harimalala, Mireille, Pierre Lefeuvre, Alexandre De Bruyn, et al. "A novel cassava-infecting begomovirus from Madagascar: cassava mosaic Madagascar virus." Archives of Virology 157, no. 10 (2012): 2027–30. http://dx.doi.org/10.1007/s00705-012-1399-3.

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Ramkat, Rose, Alberto Calari, Fatemeh Maghuly, and Margit Laimer. "Occurrence of African cassava mosaic virus (ACMV) and East African cassava mosaic virus – Uganda (EACMV-UG) in Jatropha curcas." BMC Proceedings 5, Suppl 7 (2011): P93. http://dx.doi.org/10.1186/1753-6561-5-s7-p93.

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Karthikeyan, Chockalingam, Basavaprabhu Patil, Basanta Borah, et al. "Emergence of a Latent Indian Cassava Mosaic Virus from Cassava Which Recovered from Infection by a Non-Persistent Sri Lankan Cassava Mosaic Virus." Viruses 8, no. 10 (2016): 264. http://dx.doi.org/10.3390/v8100264.

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Ogbe, F. O., G. I. Atiri, A. G. O. Dixon, and G. Thottappilly. "Symptom severity of cassava mosaic disease in relation to concentration of African cassava mosaic virus in different cassava genotypes." Plant Pathology 52, no. 1 (2003): 84–91. http://dx.doi.org/10.1046/j.1365-3059.2003.00805.x.

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Zeng, Hongqiu, Yanwei Xie, Guoyin Liu, Yunxie Wei, Wei Hu, and Haitao Shi. "Agrobacterium-Mediated Gene Transient Overexpression and Tobacco Rattle Virus (TRV)-Based Gene Silencing in Cassava." International Journal of Molecular Sciences 20, no. 16 (2019): 3976. http://dx.doi.org/10.3390/ijms20163976.

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Agrobacterium-mediated transient expression and virus-induced gene silencing (VIGS) are very useful in functional genomics in plants. However, whether these methods are effective in cassava (Manihot esculenta), one of the most important tropical crops, remains elusive. In this study, we used green fluorescent protein (GFP) and β-glucuronidase (GUS) as reporter genes in a transient expression assay. GFP or GUS could be detected in the infiltrated leaves at 2 days postinfiltration (dpi) and were evidenced by visual GFP and GUS assays, reverse-transcription PCR, and Western blot. In addition, phy

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Tadu, G., S. Winter, A. M. A. Gadelseed, and G. A. Dafalla. "Association of East African cassava mosaic virus-Uganda (EACMV-UG) with cassava mosaic disease in Sudan." Plant Pathology 55, no. 2 (2006): 287. http://dx.doi.org/10.1111/j.1365-3059.2005.01305.x.

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Dutt, N., R. W. Briddon, and I. Dasgupta. "Identification of a second begomovirus, Sri Lankan cassava mosaic virus, causing cassava mosaic disease in India." Archives of Virology 150, no. 10 (2005): 2101–8. http://dx.doi.org/10.1007/s00705-005-0579-9.

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Seif, A. A. "Epidemiology of African Cassava Mosaic Virus in Kenya." East African Agricultural and Forestry Journal 54, no. 4 (1989): 215–21. http://dx.doi.org/10.1080/00128325.1989.11663569.

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Mathew, A. V., and V. Muniyappa. "Purification and Characterization of Indian Cassava Mosaic Virus." Journal of Phytopathology 135, no. 4 (1992): 299–308. http://dx.doi.org/10.1111/j.1439-0434.1992.tb04315.x.

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Fauquet, Claude. "African Cassava Mosaic Virus: Etiology, Epidemiology, and Control." Plant Disease 74, no. 6 (1990): 404. http://dx.doi.org/10.1094/pd-74-0404.

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Roberts, I. M. "Indian Cassava Mosaic Virus: Ultrastructure of Infected Cells." Journal of General Virology 70, no. 10 (1989): 2729–39. http://dx.doi.org/10.1099/0022-1317-70-10-2729.

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Adriko, J. "Response of Local Cassava Varieties in Uganda to Cassava Mosaic Virus Disease." American Journal of Experimental Agriculture 2, no. 1 (2012): 111–32. http://dx.doi.org/10.9734/ajea/2012/736.

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