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1
Jiang, Mingkang Wang Yifen. "Development of smoked and gelatin-based products from catfish." Auburn, Ala, 2009. http://hdl.handle.net/10415/1849.
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Diffoot, Nanette. "Corydoras aeneus: a diploid-tetraploid fish species complex." Thesis, Virginia Polytechnic Institute and State University, 1986. http://hdl.handle.net/10919/101468.
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Corydoras aeneus is an armoured catfish found in the upper amazon regions of South America. It is a member of the family Callichthyidae. Studies done with this species of fish showed that C. aeneus is a tetraploid with a chromosome number of 132 (Scheel et al. , 1972). Dunham et al. (1980) reported 120 chromosomes. The C. aeneus used in this study were bought from direct importers. We had four samples of fishes supposedly coming from Brazil (Belem), Guyana, Peru and Trinidad. During our initial studies in an attempt to karyotype aeneus we came across individuals with a highly reduced chromosome number. A diploid form of C. aeneus was discovered. Only those fishes from our Belem sample were diploid. A comparison of the diploid and tetraploid forms was done. Both forms were karyotyped, the tetraploid form of C. aeneus has 134 chromosomes and the diploid has 56. Physically both forms looked exactly the same. Morphometric as well as meristic data was collected from 131 fishes and analyzed by multivariate, discriminant and contingency chi- square analyses. The results obtained do not suggest any absolute morphological differences between the diploid and the tetraploid forms anymore than between tetraploids.M.S.
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Haylor, Graham S. "The culture of African Catfish, Clarias gariepinus (Burchell) in Africa, with particular reference to controlled hatchery production." Thesis, University of Stirling, 1992. http://hdl.handle.net/1893/3267.
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A rationale is presented for a primary nursing strategy and an on-growing strategy for Clarias gariepinus (Burchell) culture in Africa, thus providing a potential model for the development of culture technology for the species. Existing information pertaining to the production strategies identified is reviewed, highlighting the attributes of African catfish for aquaculture. Some of the current deficiencies and inconsistencies in available information pertaining to controlled hatchery production are addressed. The early developmental stages of Clarias gariepinus are defined, in order to promote consistent use of terminology and help farmers better address the changing needs of their developing stock. The pattern of growth and survival of larvae and fry is investigated at higher stocking densities than those used experimentally to provide a database for planning full-scale commercial operations. Tank design and water flow rates appropriate for Clarias gariepinus in hatcheries are investigated and recommendations made. Finally, in order to promote maximal growth rates of hatchery stocks the maximum daily feed intake of larvae in relation to different feeding regimes is estimated based on rates of gastric evacuation and return of appetite. An overview of the controlled hatchery production of Clarias gariepinus is presented.
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Lückhoff, Paul Daniël. "Application of the condition factor in the production of African sharptooth catfish Clarias gariepinus /." Link to the online version, 2005. http://hdl.handle.net/10019/1098.
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Mullins, Margy 1958, and Margy 1958 Mullins. "Effect of frozen storage on some biochemical, ultrastructural, and textural properties of tilapia (Tilapia aureus) and catfish (Ictalarus punctatus) muscle." Thesis, The University of Arizona, 1986. http://hdl.handle.net/10150/625875.
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Britz, P. J. (Peter Jacobus) 1959. "Environmental requirements for the hatchery rearing of African catfish Clarias Gariepinus (Pisces: Clariidae) larvae and juveniles." Thesis, Rhodes University, 1988. http://hdl.handle.net/10962/d1001964.
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Inadequate seed production has historically been a factor limiting the commercial culture of Clarias gariepinus. The need to determine the environmental requirements of larvae was identified, in order to facilitate their successful mass rearing in hatcheries. The effects of key environmental factors on the growth, survival and aspects of the behaviour of C. gariepinus larvae and juveniles were investigated under controlled conditions. A strong emphasis was placed on the interpretation of the observed responses in terms of the natural history of the animal. Larvae were obtained by artificially inducing and spawning feral adult broodfish. Larval growth rates were highest in the temperature range 26-33ºC, with a peak at 30ºC. Survival of larvae was high between 22 and 33ºC. The final temperature preferendum of juveniles, determined in a thermal gradient was 30ºC, and it was concluded that this temperature is probably optimal for most of the physiological processes of the animal. Larvae and juveniles displayed strong negative phototaxis. When reared under different photoperiod regimes, larval growth increased with longer dark periods, however a similar rate of mortality was recorded in all photoperiod treatments. The provision of cover was found to enhance larval growth under conditions of continual light (24L/OD), however under conditions of continual darkness (OL/24D) similar growth rates were recorded regardless of the presence or absence of cover. All growth rates in continual darkness (OL/24D) were higher than those in continual light (24L/OD), regardless of whether cover was provided or not. It was concluded that a OL/24D photoperiod is optimal for larval rearing. At salinities between 0 and 5% similar growth and mortality rates of larvae were recorded. At 7.5 % larval growth and survival rates were lower, and at 10 % all larvae died within 48 hours. The 96h-LCSO for unionised ammonia was found to be 2.3 mg/l. The cytological effects of unionised ammonia were evident as degenerative changes in the gill and liver tissues. In an investigation of tank hygiene, it was found that larval growth and survival rates were highest in tanks not cleaned at all. As the frequency of tank cleaning increased, the growth and survival rates of larvae decreased. When reared at densities ranging from 45-450/1, larval growth was found to be density dependent. A model of hatchery productivity (number larvae of standard size produced/time/vol.) was developed based on the density dependent nature of larval growth. The model predicts that maximum productivity, will be achieved at a rearing density of 1400/1. The high growth and survival rates obtained over a broad range of each environmental parameter investigated serve to explain how larvae survive and grow in their unstable floodplain habitat in nature. The broad environmental tolerances of C. gariepinus are of benefit with respect to the culture of larvae, as successful larval rearing is possible over a fairly wide range of environmental conditions.
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German, Donovan Parks. "Beavers of the fish world can wood-eating catfishes actually digest wood? a nutritional physiology approach /." [Gainesville, Fla.] : University of Florida, 2008. http://purl.fcla.edu/fcla/etd/UFE0023785.
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Wudtisin, Idsariya. "Bottom soil quality in ponds for culture of catfish, freshwater prawn, and carp in Thailand." Auburn, Ala., 2006. http://repo.lib.auburn.edu/2005%20Fall/Dissertation/WUDTISIN_IDSARIYA_58.pdf.
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Fagbenro, Oyedapo Adewale. "Studies on the use of fermented fish silage in diets for juvenile tilapia (Oreochromis niloticus) and catfish (Clarias gariepinus)." Thesis, University of Stirling, 1994. http://hdl.handle.net/1893/1924.
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Fermented silage was prepared from a mixture of minced tiiapias (Oreochromis spp. ), different carbohydrate substrates (molasses, corn flour, tapioca flour) and Lactobacillus plantarum as inoculum, incubated anaerobically for 30 days at 5°-35°C. The pH and protein solubilization were temperature-dependent, and the source of carbohydrate substrate did not affect non-protein nitrogen (NPN) content or proximate composition of tilapla silage. During storage at 30°C for 180 days, NPN content increased and there was 8-11% loss of tryptophan. Moist diets containing tilapla silage (stored up to 60 days) were fed to Clarias gariepinus and differences in growth and protein utilization were demonstrated, but there were no effects on body composition. Partial replacement of fish meal with co-dried tilapla silage and soybean flour blend (FSS: BF) in dry diets supported growth and protein utilization similar to the control treatment. Fish growth and protein utilization were reduced with total replacement of fish meal. Apparent protein digestibility decreased with Increasing dietary level of co-dried FSS: BF. Carcass composition was not affected and morphological defects were not observed. Co-dried tilapla silage blended with soybean meal, poultry by-product meal, hydrolysed feather meal or meat and bone meal (FSS: BM, FSP: BM, FSH: FM, FSM: BM) (providing 50% of the dietary protein) In dry diets fed to Oreochromis niloticus and Clarias gariepinus gave differences In growth, protein utilization and digestibility, and apparent energy digestibility. Carcass composition was not affected by silage blend and histological examination of exocrine pancreas, liver and Intestine tissues did not show any lesions suggestive of nutritional imbalance. Haematocrit, haemoglobin content and mean cell haemoglobin concentration values showed no differences among the treatments. The results indicated that fermented tilapla silage is a suitable protein supplement in moist or dry diets for Oreochromis niloticus and Clarias gariepinus, without affecting feed efficiency, fish growth or health.
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Luckhoff, Paul Daniel. "Application of the condition factor in the production of African sharptooth catfish Clarias gariepinus." Thesis, Stellenbosch : University of Stellenbosch, 2005. http://hdl.handle.net/10019.1/3421.
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Thesis (MPhil (Animal Sciences. Aquaculture))--University of Stellenbosch, 2005.In recent years there has been a renewed interest in the commercial culture of African Sharptooth Catfish. Its robust characteristics and its air breathing capabilities makes the African catfish a good candidate for culture in intensive recirculating systems. In light of the size variation in offspring spawned from undomesticated fish, that may eventually increase cannibalism, suitable methods for the quantification of some production performance parameters such as growth and health measurements need to be established for application in intensive catfish culture. In fish the condition factor (CF) reflects information on the physiological state of the fish in relation to its welfare. This factor is expressed as Fulton’s condition index, or the K-factor. African Sharptooth Catfish fry with average weight of 1.9g ± 0.7867 and average length of 59.375mm ±8.812 were equally allocated into ten 1000L-recirculating tanks. The fish were fed a commercial feed to apparent saturation at a frequency of 5 times per day. Sixteen fish were sampled out of each treatment. Weight (g) and length (mm) of each fish were recorded every seven days over a trial period of 175 days from which Fulton’s condition index K was calculated. Results were analysed for significant differences using one-way ANOVA and Tukey’s pair wise comparison test for the various parameters. Growth parameters (a = -5.083, b = 3.004, R2 = 99.4%) derived from the logarithmic relationship between body weight (W) and standard length (L) indicated an isometric growth through the duration of the trail. No significant differences (P>0.05) in condition factors between treatments were found at the beginning of the trail. Significant differences (P<0.05) were found at the end of the trail for weight between ponds and condition factor between treatments. No significant difference (P> 0.05) between length and treatments were found at the end of the trial. Mean condition factor was 0.856 ± 0.187. By using this information on condition factor for African catfish a K-factor calculation chart for African catfish could be calculated, to be used as a practical measurement tool to measure performance goals on catfish farms.
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Willenberg, Zachary J. "Selected population characteristics of channel catfish, Ictalurus punctatus, and flathead catfish, Pylodictis olivaris, in the lower 200 miles of the Wabash River." Virtual Press, 2000. http://liblink.bsu.edu/uhtbin/catkey/1191726.
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This study describes the population characteristics of channel catfish (Ictalurus punctatus) and flathead catfish (Pylodictis olivaris) in the lower 200 miles of the Wabash River in 1998. Densities of channel and flathead catfish did not differ over the study reach for either species. Electrofishing captured twice as many channel as flathead catfish, and hoop nets captured twice as many flatheads as channel catfish. Growth rates of channel catfish were fast as compared to documented growth in other rivers in the United States, with ages ranging from 1-8. Flathead catfish growth was average when compared to that reported in other United States rivers with ages ranging from 0-7. Length-frequency analysis for both species revealed the populations were comprised of small individuals with mortality higher than expected in older fish. This study will aid in the management of the Wabash River, as historic data are limited.Department of Biology
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Sands, David Dean. "The behavioural and evolutionary ecology of Corydoras adolfoi and Corydoras imitator : studies on two sympatric species of catfishes from a small tributary of the Upper Rio Negro, Brazil (Pisces, Siluriformes, Callichthyidae)." Thesis, University of Liverpool, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.240810.
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Luu, Truc T. T. "Investigation into jaundice in farmed catfish (Pangasianodon hypophthalmus, Sauvage) in the Mekong Delta, Vietnam." Thesis, University of Stirling, 2013. http://hdl.handle.net/1893/13060.
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Disease outbreaks continue to be a major problem in the sustainable development of the aquaculture industry. Clinical outbreaks can negatively impact on the welfare of the fish and the economic gain derived from this industry. Jaundice observed as a yellow colouration in the abdominal skin, sclera of the eyes and fin bases is a significant health problem affecting the Vietnamese freshwater catfish industry. This study was designed to investigate jaundice of farmed catfish, Pangasianodon hypophthalmus using several complementary approaches. These included clinical investigations and identification of potential aetiological agents as well as epidemiological analyses to identify farm-based risk factors for this economically devastating condition occurring in the catfish farms of the Mekong Delta. The results of this survey demonstrated that the jaundice was not linked to a single geographical location as affected fish were found widely distributed throughout the five main production areas. Nor was any association found between any weight groupings, feed type or feeding regime applied in the affected farms. The highest prevalence occurred between June to October and fish mortalities ranged from 1 to 10% in the study sites. The duration of this condition was significantly correlated (P < 0.05) to mortality but not to total farm area, depth of pond, stocking density, or amount of water exchanged. The number of fish ponds affected was not as high in the large-scale farms compared to the small-scale farms. The results from the clinical description study showed that the affected fish were suffering a form of jaundice or icterus. Histological examination revealed a number of serious pathologies in the affected fish. Spleenomegaly was associated with the loss of cell structure and connective tissue and the haematopoietic tissue had large areas of necrosis. In the liver, histological changes consisted of vasculitis and multifocal to diffuse hepatocellular necrosis. The presence of haemosiderin was observed in melano-macrophage centres in the spleen and kidney of jaundiced fish. No single pathogen was identified in the jaundiced fish. Myxosporean infection was found in both apparently normal fish and jaundiced fish. However, there was a definite tendency for jaundiced fish to be more heavily infected. Histopathological examination found several changes that could not be ascribed to specific aetiological factors and presume that both groups (jaundiced alone and myxosporean-affected jaundiced fish) have similar lesions. The results of this study would suggest that the parasite identified as M. pangasii was not a primary pathogen associated with the haemolytic jaundice. Neither were the gills myxosporeans associated with the haemolytic jaundice and they may be considered more as a nuisance rather than as primary pathogens in farmed P. hypophthalmus in the Mekong Delta, Vietnam. Univariate analysis of the whole dataset showed several variables were significantly associated with the haemolytic jaundice. However, none of the variables achieved lasting statistical relevance in multivariable models. In conclusion, this study identified a haemolytic jaundice condition affecting farmed P. hypophthalmus in Vietnam, but no single aetiological agent or farm based risk factor was identified with this condition. Several priority areas for further work were identified and include a prospective, longitudinal cohort study to identify further the risk factors associated with the clinical jaundice condition.
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Jongrattananon, Saowalee. "STABILITY CATFISH METHYL ESTERS UNDER NORMAL VERSUS ACCELERATED STORAGE CONDITIONS." MSSTATE, 2008. http://sun.library.msstate.edu/ETD-db/theses/available/etd-10292008-214838/.
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Oxidative storage stability was conducted to determine the optimum antioxidant for the production of catfish methyl esters (CFME) for use as biodiesel. Peroxide value, anisidine value, 2-thiobarbituric reaction substances, acid value, iodine value, UV absorbance, and induction period were measured. Antioxidant, stability parameters changed over a storage time of one year when stored at 25°C. The CFME were not stable to oxidation without the addition of TBHQ. When stored at 25°C, oxidative parameters indicated that CFME was a stable product that met oxidative stability standards when TBHQ was added. When held at 80°C for 28 days, the samples with TBHQ were stable to oxidation. However, the acid value rose above the maximum at 28 days. Thus, CFME can be stable to oxidative breakdown when THBQ was added at 800 ppm, but when exposed to high temperatures, one will have to limit water or protect CFME against hydrolytic rancidity.
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Fortune, Shelby E. "Evaluation of automated, manual and constant aeration practices in managing of dissolved oxygen for channel catfish farming in earthen ponds." Master's thesis, Mississippi State : Mississippi State University, 2008. http://library.msstate.edu/etd/show.asp?etd=etd-07182008-143119.
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Triyono, Sugeng. "Continuous simulation of groundwater use and effluent discharge in catfish (Ictalurus punctatus) ponds at five locations in the Southeast U.S." Diss., Mississippi State : Mississippi State University, 2007. http://library.msstate.edu/etd/show.asp?etd=etd-05292007-185838.
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Cavalet, Elenita. "Revisão anatômica e osteológica de Parotocinclus maculicauda (Steindachner, 1877) (Loricariidae: Hypoptopomatinae)." Universidade do Vale do Rio dos Sinos, 2014. http://www.repositorio.jesuita.org.br/handle/UNISINOS/3023.
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Milton Valente
A família Loricariidae compreende o maior número de espécies descritas de cascudinhos com aproximadamente 872 espécies válidas, sendo a maior família da ictiofauna mundial. A subfamília Hypoptopomatinae composta por 20 gêneros e 136 espécies é reconhecida como monofilética. Entretanto, a monofilia do gênero Parotocinclus, (Lehmann, 2006) vem sendo contestada. Estudos filogenéticos afirmam que Parotocinclus ocupa uma posição mais basal dentro tribo Otothyrini. Em uma hipótese mais recente da filogenia da subfamília, não foi encontrado suporte para apoiar o monofiletismo da tribo Otothyrini, e Parotocinclus foi reconhecido como um gênero agrupando um complexo de espécies altamente polifilético. Atualmente, Parotocinclus agrupa 28 espécies válidas. Parotocinclus maculicauda (Steindachner, 1877) (espécie tipo do gênero) é encontrada nos rios costeiros desde Santa Catarina até o Espírito Santo. Caracteriza-se pela presença de placas ósseas, cintura peitoral exposta suportando odontódeos, nadadeira adiposa presente e nadadeira dorsal e peitoral um pouco avermelhada. Apresenta dimorfismo sexual, machos com papila genital visível e uma projeção de pele na base do espinho na nadadeira pélvica. O esqueleto de P. maculicauda apresenta alto grau de fusão e redução de tamanho em muitas estruturas ósseas. A análise de caracteres externos e internos e, sua comparação entre as diferentes espécies de Loricariideos, assim como a configuração dos ossos e músculos associados à cintura peitoral e ao crânio, são considerados no levantamento de caracteres morfológicos informativos a diferentes níveis dentro da família Loricariidae.
The family Loricariidae comprises the largest number of described species of catfishes with approximately 872 valid species , the largest family of world fish fauna. The Hypoptopomatinae subfamily comprises 20 genera and 136 species are recognized as monophyletic . However , the monophyly of the genus Parotocinclus, is being challenged . Phylogenetic studies claim that Parotocinclus occupies a more basal position within Otothyrini tribe. In a more recent hypothesis of the phylogeny of the subfamily, was not found support for the monophyly of Otothyrini tribe and Parotocinclus was recognized as a genera gathering a complex of highly polyphyletic species. Currently , Parotocinclus joins 28 valid species. Parotocinclus maculicauda (type species of the genus) is found in coastal rivers from Santa Catarina to the Espirito Santo states . It is characterized by the presence of bony plates, pectoral girdle exposed supporting odontodes , the presence of adipose fin and dorsal and pectoral fins slightly reddish . Was sexually dimorphic, males with visible genital papilla, a projection of skin at the base of the spine in the pelvic fin. The skeleton of P. maculicauda presents a high degree of mergers and downsizing in many bony structures. The analysis of external and internal characters and their comparison between different loricaiid species, as well as the configuration of the bones and associated pectoral girdle and skull muscles, are considered in the survey of morphological information at different levels within the family Loricariidae.m
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Jacob, Annie Philip. "Ecological effects of chemicals used in pond culture of catfish and percid fishes." Columbus, Ohio : Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1211989438.
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Kadye, Wilbert Takawira. "Assessing the impacts of invasive non-native African sharptooth catfish Clarias Gariepinus." Thesis, Rhodes University, 2012. http://hdl.handle.net/10962/d1005070.
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Invasive species are of particular concern as they have the potential to alter community structure and food web relationships within their invaded habitats. African sharptooth catfish Clarias gariepinus, a generalist predator, was introduced through an inter-basin water transfer scheme into the Great Fish and Sundays Rivers, Eastern Cape, South Africa, where it threatens the native riverine biota. This thesis assessed its impact from a trophic perspective. Patterns in catfish distribution and abundance revealed an upstream to downstream gradient that was associated with spatial distribution of most species within the mainstream, and a mainstream to tributary gradient that was associated with the spatial distribution of native minnows. The catfish was predicted to occur widely within the mainstem habitats and to decrease progressively along the mainstrem to tributary gradient with the physico-chemical environment being a good proxy for predicting both its occurrence and abundance. The results suggest the catfish proliferated within mainstem habitats where invasion resistance was possibly reduced due to alteration of flow. Population dynamics and size structuring of two native cyprinid minnows Pseudobarbus afer and Barbus anoplus, threatened by catfish, were examined within uninvaded headwater streams in relation to their proximate physical habitats. Their habitats were characterised by seasonal variation in physico-chemical conditions and a spatial variation in substrata compositions. No evidence of differences was found between seasons for density and capture probability for either species. The population size and density for P. afer was found to increase with increasing proportion of boulders. In comparison, B. anoplus population size and probability of capture increased with increasing proportion of bedrock and bank vegetation, respectively. Size structuring was explained predominantly by seasonality and habitat variables for P. afer and B. anoplus, respectively. Stable isotope ratios of carbon and nitrogen were used to compare the spatial variation in both the community-wide and catfish-specific niches and to estimate catfish prey sources from different habitats within the invaded systems. Aquatic community and catfish niches were statistically different among localities, suggesting that each locality had a distinct community-wide trophic structure. Dispersion metrics indicated no evidence of differences in the clustering among individuals, but provided evidence of differences in path trajectories for the comparisons of catfish populations that suggested dietary plasticity within different localities. Dietary studies revealed both ontogenetic shift and omnivory that suggested that catfish may exhibit less pronounced top-down effects within its invaded habitats. Manipulative experiments were used to test the response of benthic macroinvertebrates within two rivers that were differentially impacted by catfish as a presstype disturbance. Macroinvertebrates were non-responsive to catfish presence within a system where catfish had previously been established. In contrast, excluding catfish in this system indicated a response that suggested the importance of refuge within invaded habitats and the possible recovery pattern of certain macroinvertebrate taxa. By comparison, introduction of catfish within previously uninvaded localities provided evidence of direct catfish impact through elimination of conspicuous taxa. Acoustic telemetry was used to investigate catfish movement patterns within an invaded lentic habitat and provided evidence that habitat utilisation was non-random. The shallow and structured river mouth habitat, which was most utilised, was probably the most ideal for its breeding and feeding. This inferred potential overlap with native species and suggested the risk of predation and competitive interference. Catfish also exhibited both nocturnal and diurnal activity patterns that were probably related to feeding.
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Mpofu, Christopher. "Assessment of organochlorine pesticide residues in fish samples from the Okavango Delta, Botswana." Thesis, Rhodes University, 2011. http://hdl.handle.net/10962/d1006707.
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This thesis presents an evaluation of the dispersive solid-phase extraction (d-SPE) method referred to as the quick, easy, cheap, effective, rugged and safe (QuEChERS) method for the determination of four organochlorine pesticide residues in fish samples. The pesticides investigated in this study were o, p′-DDT, p, p′-DDE, aldrin and dieldrin. The combined use of Gas Chromatography with an Electron Capture Detector (GC-ECD) and sensitive Time of Flight (TOF) mass detector facilitated the identification of the target analytes. In the absence of certified reference material, the overall analytical procedure was validated by systematic recovery experiments on spiked samples at three levels of 2, 5 and 10 ng/g. The targeted compounds were successfully extracted and their recovery ranged from 76 to 96% with relative standard deviations of less than 13%. The optimum QuEChERS conditions were 2g of fish powder, 10 ml acetonitrile and 1 min shaking time. The optimal conditions were applied to assess the levels of chlorinated pesticides in blunt-tooth catfish (Clarias ngamensis), tigerfish (Hydrocynus vittatus), Oreochromis andersonii and red-breasted tilapia (Tilapia rendalli) from the Okavango Delta, Botswana. Dieldrin, p, p′-DDE and aldrin were detected in all the analysed samples with a concentration range of 0.04 – 0.29, 0.07 – 0.33, 0.04 – 0.28 and 0.03 – 0.24 ng/g per dry weight in O. andersonii, C. ngamensis, T. rendalli and H. vittatus respectively. These concentrations were below the US-EPA 0.1 μg/g allowable limit in edible fish and the Australian Maximum Residue Limit (MRL) of 50 - 1000 ng/g in fresh fish. DDT was not detected in all the fish species investigated. The mean lipid content recorded in the fish samples were 1.24, 2.16, 2.18 and 4.21% for H. vittatus, T. rendalli, O. andersonni and C. ngamensis respectively. No systematic trend was observed between fish age and pesticide levels in fish. Acetylcholinesterase (AChE) activity assays were performed to assess the effects of organochlorine pesticides in Clarias ngamensis. The enzyme activity recorded in Clarias ngamensis from the Okavango Delta and the reference site was 12.31 μmol of acetylcholine iodide hydrolysed/min/g brain tissue. The enzyme activity remained the same indicating no enzyme inhibition. The conclusions drawn from this study are that the QuEChERS method is applicable for the determination of organochlorine pesticide residues in fish matrices. The fish from the Okavango Delta are safe for human consumption.
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Barson, Maxwell. "Endoparasites of the sharptooth catfish, Clarias gariepinus (Burchell), from the Rietvlei Dam, Sesmyl Spruit system, South Africa." Thesis, 2008. http://hdl.handle.net/10210/1011.
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The Rietvlei Dam near Pretoria, South Africa, provides drinking water for the city and for the wild life in the Rietvlei Nature Reserve, and is also used for recreational fishing. The dam is part of the Sesmyl Spruit system, which has a history of pollution, the major sources of which are industrial, agricultural and sewage from informal human settlements upstream of the reserve. With a large wetland separating the upstream Marais Dam and the Rietvlei Dam, the system has a high conservation priority status because of the high number of bird species that breed and roost in the various habitats. As part of a big aquatic health project in the Zoology Department, Rand Afrikaans University, aimed at finding suitable biomarkers for water quality monitoring in the system, this study was done to identify the major internal helminth parasites of the sharptooth catfish, Clarias gariepinus, that can be used in fish health assessment studies, and to determine their prevalence and intensity in the Rietvlei Dam. Fish were collected during one sampling survey and examined for endoparasites, also noting any ectoparasites that are recorded in routine fish health studies. Five species of helminths were identified: the adult cestodes, Polyonchobothrium clarias (intestine and stomach), Proteocephalus glanduliger (anterior intestine), the adult nematode Procamallanus laevionchus (stomach), larvae of the nematode Contracaecum sp. and many trematode metacercariae encysted in the muscles, of which only Ornithodiplostomum sp. was successfully excysted and identified. This trematode is recorded in South Africa for the first time, but could not be specifically identified because the reproductive system was still immature. Examination of piscivorous birds in the area or experimental infection of young birds are the only means by which the adult trematodes can be obtained. The adult cestodes and nematodes had specialised structures for attachment to the stomach and/or intestinal mucosa, adaptations associated with pathological effects in the host. Polyonchobothrium clarias had a crown of 26-30 hooks on its rostellum, and this number differs from those of specimens described from catfish in other African countries. Scanning electron microscopy showed that the rostellum of the P. clarias specimens from Rietvlei Dam was different from that of specimens from other localities in South Africa. Proteocephalus glanduliger in C. gariepinus from Rietvlei Dam differed in strobila size and size of glandular organ from specimens described by Janicki (Egypt) and Mashego (South Africa), the present specimens being much longer but with smaller glandular organs. Procamallanus laevionchus is a common parasite of catfish from many African countries, including South Africa, and scanning electron microscopy showed some form of transverse markings and presence of papillae-like structures at the posterior end of female specimens, an observation which was not described in previous studies. Larval Contracaecum are also common in C. gariepinus and other fish species, and adults have been identified in several species of fish-eating birds from South Africa. The sample size of fish collected in this survey was too low for a full health assessment index (HAI) study to be undertaken. Polyonchobothrium clarias and Contracaecum, however, were highly prevalent in the host species, and Contracaecum and Ornithodiplostomum occurred at high intensity (up to 44 and 140 respectively). Endoparasites of C. gariepinus can therefore be used in the fish HAI as a bioindicator of water quality. Only two ectoparasitic species were found on C. gariepinus: Argulus japonicus (skin and fins) and Lamproglena clariae (gills). Most water quality variables from the dam were within the target limits recommended by the Department of Water Affairs and Forestry, but the levels of inorganic nitrogen (nitrate and ammonia) and phosphorus (orthophosphate) exceeded the limit. If uncontrolled, these may lead to eutrophication of the dam. With the parasite species and diversity known, it is recommended that fish health assessments should be conducted along pollution gradients in the system to determine whether it can be incorporated into the suite of biomarkers for water quality monitoring of the Sesmyl Spruit system.Prof. A. Avent-Oldewage
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Botha, Tarryn Lee. "A histology-based fish health assessment to determine the health and reproductive status of Clarias gariepinus in the Hartbeespoort Dam, South Africa." Thesis, 2014. http://hdl.handle.net/10210/9183.
Full textAbstract:
M.Sc. (Zoology)The freshwater indicator fish species, Clarias gariepinus, was used to assess and compare the health status of fish from the Hartbeespoort Dam (HBPD) and the Groot Marico Bosveld Dam (GM) which was used as a reference site. HBPD is known to be polluted and renowned for its extreme eutrophic state. GM receives water from the Marico River and is said to be in an unmodified natural ecological state. Adult Clarias gariepinus were sampled using gill nets; during low-flow (n=17) and high-flow (n=20) seasons from the HBPD, and once from the GM (n = 20). A histology-based fish health assessment was done using a necropsy based health assessment index and a qualitative and semi-quantitative histological assessment on selected target organs namely the gills, liver, kidney and gonads. Focus was also placed on reproductive health aspects by staging the gonads according to their reproductive development and assessing the motility of activated sperm using computer assisted sperm analysis (CASA). Water samples were analyzed for selected physical parameters and for selected metals. The results showed distinct macroscopic differences in the livers and testes comparing the two sites. Fish from HBPD had fatty livers and the macroscopic morphology of the testes showed abnormalities regarding the interstitial tissue, possibly due to the proliferation of connective tissue. The macroscopic abnormalities of the livers were reflected in the histological assessment, which concluded steatosis, vacuolation, hepatocyte nuclear alterations and the presence of large numbers of melanomacrophage centers (MMCs). Of these alterations, only MMCs and intracellular deposits were found in fish from the GM. When comparing the HBPD low-flow (LF) and high-flow (HF) sampling trips, the fish from the low-flow had more alterations present in all organs. While the CASA results showed that the motility, velocity and progression of sperm were lower in fish from the HBPD for all parameters, results from GM showed the ideal trend expected from the moment of sperm activation until degeneration. The velocity and progression were significantly (p value <0.05) different between HBPD samples and fish from the GM. The water quality showed increased concentrations of selenium, as well as the endocrine disrupting chemicals (EDCs) nonylphenol and di-n-butyl phthalate. According to the selected parameters assessed, it seems like the water of the HBPD has increasing detriment upon fish health.