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Journal articles on the topic 'Cattle Immunoglobulins. Serum Immunochemistry'
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Wray, C., J. A. Morris, and W. J. Sojka. "Immunoglobulins Detected by the Serum Agglutination Test for Salmonella dublin Infection of Cattle." Zentralblatt für Veterinärmedizin Reihe B 26, no. 4 (2010): 340–43. http://dx.doi.org/10.1111/j.1439-0450.1979.tb00821.x.
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Ezdakovа, I. Yu, and V. F. Polyakov. "The level of immunoglobulins in the blood serum of various groups of cattle." "Veterinary Medicine" Journal 21, no. 7 (2018): 21–24. http://dx.doi.org/10.30896/0042-4846.2018.21.7.21-24.
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van Gijlswijk, Rob PM, Eduard G. Talman, Inge Peekel, et al. "Use of Horseradish Peroxidase- and Fluorescein-modified Cisplatin Derivatives for Simultaneous Labeling of Nucleic Acids and Proteins." Clinical Chemistry 48, no. 8 (2002): 1352–59. http://dx.doi.org/10.1093/clinchem/48.8.1352.
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Abstract Background: Microarray platforms will change immunochemical and nucleic acid-based analysis of cell homogenates and body fluids compared with classic analyses. Microarrays use labeled target and immobilized probes, rather than fixed targets and labeled probes. We describe a method for simultaneous labeling of nucleic acids and proteins. Methods: Horseradish peroxidase- and fluorescein-modified cisplatin derivatives were used for labeling of nucleic acids and proteins. These reagents, called the Universal Linkage System (ULS), bind to sulfur- and nitrogen-donor ligands present in amino acids and nucleotides. For automated screening of proteins and nucleic acids on microarrays, it is advantageous to label these biomolecules without pre- or postpurification procedures. The labeling of antibodies and nucleic acids in whole serum was therefore pursued. Results: Immunoglobulins in nonpurified serum were labeled efficiently enough to be used for immunochemistry. To investigate whether protein-adapted labeling allowed nucleic acid labeling as well, 1 μg of plasmid DNA was added to 1 μL of serum. DNA and serum proteins were simultaneously labeled, and this labeled DNA could be used as a probe for direct fluorescence in situ hybridization. Conclusion: ULS provides a direct labeling tool for the (simultaneous) modification of proteins and nucleic acids even in unpurified samples.
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Semenov, V. G., D. A. Baimukanov, A. S. Alentayev, N. K. Kirillov, A. K. Karynbayev, and A. V. Aldyakov. "FACTORS OF NONSPECIFIC RESISTANCE OF CALVES IN DAIRY CATTLE BREEDING." BULLETIN 389, no. 1 (2021): 81–88. http://dx.doi.org/10.32014/2021.2518-1467.11.
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Under the influence of the biostimulant, an increase in the morphological and biochemical data of blood in calves grown both on private plots and on small and medium farms, which at the end of the observation period exceeded the control values: the number of red blood cells - by 0.98; 0.81 and 0.79x1012/l, hemoglobin - by 9.8; 7.0 and 6.2 g/l, albumins - by 2.2; 2.8 and 3.5 g/l, γ-globulins - by 5.5; 3.7 and 5.6 g/l (P <0.05-0.001), respectively. The data on the leukocyte phagocytic activity, plasma lysozyme activity, serum bactericidal activity, and immunoglobulins were higher than in the control: when rearing calves in private subsidiary plots - by 5.6%, 4.8%, 5.5% and 4.7 mg/ml, on a small farm - by 5.2; 4.2; 7.8 % and 3.6 mg/ml and on a medium farm - by 5.8%, 3.6%, 4.4% and 4.3 mg/ml, respectively (P<0.05-0.01). A rising in the technological stress on the calf’s organism was revealed depending on the increase in the enterprise capacity, which is confirmed by the bioamine blood spectrum.
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Smirnov, P. N., S. M. Chudum, I. V. Trostyansky, and O. S. Kotlyarova. "MORPHOLOGICAL CHARACTERISTICS OF PERIPHERAL BLOOD OF COWS INFECTED WITH BLV AND THE FREQUENCY OF REGISTRATION OF LEUKEMOID REACTIONS IN COWS WITH LEUKEMIA-AFFECTED HERDS." Innovations and Food Safety, no. 1 (June 30, 2020): 66–72. http://dx.doi.org/10.31677/2311-0651-2020-27-1-66-72.
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In controlled experiments during planned studies of cattle for leukemia, animals that showed leukemoid changes in blood – quantitative redistribution of granulocytes and agranulocytes-were identified. Individual analysis revealed that granulocytosis was detected in animals with inflammatory processes. In addition, the article presents comparative indicators of serum proteins in cows at the hematological stage of the leukemic process, with the manifestation of leukemoid reactions and in clinically healthy cows. Characteristic changes in the synthesis of immunoglobulins in cows with leukemia and leukemoid changes in the morphological composition of blood were established. With successful treatment of inflammatory processes, the hematological status of cows is restored to the initial indicators.
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Šoltésová, Helena, Veronika Nagyová, Csilla Tóthová, and Oskar Nagy. "Haematological and blood biochemical alterations associated with respiratory disease in calves." Acta Veterinaria Brno 84, no. 3 (2015): 249–56. http://dx.doi.org/10.2754/avb201584030249.
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Respiratory diseases of cattle, particularly in young animals, represent the most important health and economic problem of cattle rearing. The aim of the present study was to evaluate the changes of selected blood indices in 25 calves aged 2–9 months suffering from etiologically undifferentiated chronic respiratory diseases. Blood samples were analysed for haematological indices and selected serum biochemistry variables. The results found in sick animals were compared with results from 25 healthy animals of the same age, housing and feeding system. Significant differences in means between the groups of clinically healthy and sick calves were found in 13 out of 24 evaluated indicators. In sick animals we found significantly higher mean concentrations of haemoglobin and total number of white blood cells (P < 0.05 and P < 0.01, respectively), as well as higher mean activity of AST and LDH (P < 0.01), concentrations of total proteins and total immunoglobulins (P < 0.001). Significantly lower mean values were recorded in the serum concentrations of albumin, creatinine and glucose (P < 0.001), as well as in the concentration of Mg (P < 0.01), P (P < 0.001), Fe and Zn (P < 0.05). The presented results suggest the effect of respiratory diseases in calves on several changes of haematological and selected serum biochemical indicators. They indicate that respiratory diseases did not lead only to direct disturbance of gas exchange and acid-base balance, but they also indirectly affect some other variables of blood biochemistry.
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Filippo, Paula A. Di, Saulo T. Lannes, Marcos A. D. Meireles, Andressa F. S. Nogueira, and Célia R. Quirino. "Concentrations of acute-phase proteins and immunoglobulins in serum and synovial fluid in clinically healthy heifers and steers." Pesquisa Veterinária Brasileira 39, no. 6 (2019): 388–92. http://dx.doi.org/10.1590/1678-5150-pvb-5817.
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ABSTRACT: The aim of the study was to determine the concentration pattern of intra-articular acute phase proteins (APPs) and immunoglobulins in healthy crossbred cattle. Synovial fluid (SF) samples were collected from the radiocarpal joint of 25 heifers and 25 steers. Concentrations of APPs were measured by SDS-PAGE. The results were submitted to analysis of variance using the SAS statistical program, and means were compared by the Student-Newman-Keuls test (P<0.05). Thirty-seven proteins with molecular weights ranging from 7 to 37kDa were identified in SF of all animals. Eight were nominally identified with immunoglobulin A (IgA) and G (IgG), ceruloplasmin (Cp), transferrin (Tf), albumin (Ab), α1-antitripsin (AAT), α1-acid glycoprotein (AGP), and haptoglobin (Hp). The α1-antitripsin was only identified in the Sf of the heifers. The SF values of Cp, Hp, AGP and IgA were significantly higher in heifers than in steers. In sera, 34 proteins with molecular weights between 7 and 244kDa were identified in heifers and steers. Similar proteins were nominally identified in the sera, however the α1-antitrypsin was identified only in SF. The serum values Tf, AGP and IgG were significantly higher in heifers compared with steers. In conclusion, the physiological acute-phase proteins concentrations in synovial fluid of healthy ruminants can be useful in the interpretation of samples from animals with joint diseases. The SF electrophoretic profile of healthy ruminants differs depending on gender. Similar proteins were nominally identified in the sera, but only the SF of α1-antitrypsin.
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Semenov Vladimir Grigoryevich,, Yelemesov Kopmagambet Yelemesovich,, Alentayev Aleidar Saldarovich,, Tyurin Vladimir Grigorievich, та Baimukanov Aidar Dastanbekovich,. "ADAPTOGENESIS AND BIOLOGICAL POTENTIAL OF CATTLE ON СOMMERCIAL DAIRY FARM". BULLETIN 6, № 388 (2020): 85–92. http://dx.doi.org/10.32014/2020.2518-1467.186.
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The possibility of activating adaptive processes and organism resistance of cattle on commercial dairy farms under the influence of biological stimulants (polystim and PV-1) is proved. Biostimulants reduced the risk of gynecological disorders in cows: they reduced the retention time of placenta and subinvolution of uterus, reduced the endometritis and mastitis occurrence; increased reproductive function: shortened the time of the first estrus coming, increased the fertility, reduced the conception rate and duration of the service period, and improved the physicochemical composition of colostrum. The used biostimulants contributed to the increase in live weight of calves while increasing the relatively high level of assimilative processes associated with the fact that their forage energy was mainly spent on increasing body weight. At the same time, in calves not injected with biological preparations, it was mainly spent on providing homeothermia (i.e., maintaining constant body temperature), which was especially evident at low external temperatures. In calves raised in the conditions of intensive technology in winter, after the administration of dostim and polystim, the following indicators were significantly higher: the phagocytic activity of leukocytes by 5.4% - 6.4%, the lysozyme activity of plasma - by 3.0% - 6.2%, the blood serum bactericidal activity - by 7.1% - 9.5% and the content of immunoglobulins - by 2.5 mg/ml - 3.1 mg/ml. With the adaptive technology, the data of these indicators were higher: in winter period - by 5.2 - 6.4%, 3.1 - 6.2%, 6.0 - 8.7% and 4.7 - 5.7 mg/ml; and in spring-summer period - by 0.2 - 0.6%, 4.6 - 5.7%, 4.9 - 7.2% and 3.4 - 4.8 mg/ml (P<0.05-0.001), respectively.
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Braz, Lúcia M. A., Vicente Amato Neto, Clara I. L. Ferrari, et al. "Human cryptosporidiosis: detection of specific antibodies in the serum by an indirect immunofluorescence." Revista de Saúde Pública 30, no. 5 (1996): 395–402. http://dx.doi.org/10.1590/s0034-89101996000500001.
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Cryptosporidium sp., a coccidian parasite usually found in the faeces of cattle, has been recently implicated as an agent of human intestinal disease, mainly in immunocompromised patients. In the study realized, by an indirect immunofluorescence technique, specific immunoglobulins (IgG and IgM) have been demonstrated in human serum against Cryptosporidium oocysts. Purified oocysts were used as antigens in the indirect immunofluorecence assay. After analyzing this test in sera from selected groups of patients, the frequency of both specific IgG and IgM of immunocompetent children who were excreting oocysts in their faeces was 62% and in children with negative excretion of oocysts was 20% and 40%, respectively. In adults infected with the human immunodeficiency virus (HIV) and who were excreting Cryptosporidium in their stools, the frequency was 57% for IgG but only 2% for IgM. Twenty three percent of immunocompromised adults with not determined excretion of oocysts in their stools had anti-Cryptosporidium IgG in their sera. Children infected with human immunodeficiency virus had no IgM and only 14% had IgG detectable in their sera. The indirect immunoflorescence assay, when used with other parasitological techniques appears to be useful for retrospective population studies and for diagnosis of acute infection. The humoral immune response of HIV positive patients to this protozoan agent needs clarification.
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TYLER, JEFF W., JEFF LAKRITZ, DOUGLAS E. HOSTETLER, et al. "Effect of pasteurization at 76 and 63 °C on the absorption of colostral IgG in calves." Journal of Dairy Research 67, no. 4 (2000): 619–23. http://dx.doi.org/10.1017/s0022029900004441.
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The timely ingestion and absorption of colostral immunoglobulin is a critical determinant of neonatal calf health. Calves are born without appreciable concentrations of the serum immunoglobulins needed to protect against pathogenic bacteria, viruses and protozoa (Tyler & Parish, 1995). The beneficial effect of passive transfer of colostral immunoglobulin also extends beyond the neonatal period and persists into juvenile and adult life (Robison et al. 1988; Tyler et al. 1998; DeNise et al. 1989). Calves with failure of passive transfer, defined as serum protein < 50 g/l or serum IgG < 10 g/l, have increased mortality risks that persist until 10 weeks of age (Tyler et al. 1998).Several diseases are potentially spread by the ingestion of colostrum, including bovine leukosis and Johne's disease (Perrin & Polack, 1988; Streeter et al. 1995). In one study 22% of latently infected cows were demonstrated to shed Mycobacterium paratuberculosis in their colostrum (Streeter et al. 1995). Optimal programmes to prevent and eradicate these diseases generally include the provision that calves are given colostrum derived from cows of known negative disease status. Pasteurization or heat treatment of colostrum may provide a mechanism whereby calves are provided with protection against neonatal disease without creating undue potential for infection by chronic, economically relevant diseases.Attempts to heat disinfect colostrum are common in goat herds (MacKenzie et al. 1987). Pasteurization has been demonstrated to be effective against the caprine arthritis–encephalomyelitis virus (Adams et al. 1983; MacKenzie et al. 1987). Although Myco. paratuberculosis appears to resist pasteurization, this form of processing has been demonstrated to decrease the likelihood of positive colostral cultures for Myco. paratuberculosis under experimental conditions (Meylan et al. 1996). Sterilizing the milk and colostrum given to calves is a logical and reasonable strategy to prevent transmission of infectious microorganisms. The potential disadvantage of heat treating colostrum is that the immunoglobulins in colostrum may become denatured (Smith & Sherman, 1994). Pasteurization causes only a slight decrease in the colostral concentration of IgG in cattle (Meylan et al. 1996); however, the biological behaviour of these pasteurized immunoglobulins has not been critically examined. Therefore, we cannot be completely confident that immunoglobulin absorption, persistence in serum and biological activity are unchanged by this processing.The goal of this study was to determine the effect of pasteurization at 76 and 63 °C on the absorption of IgG from colostrum. Should these procedures decrease immunoglobulin absorption, the use of pasteurization in disease eradication programmes would require increased efforts to optimize the passive transfer of immunoglobulin.
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Lavryshyn, Y. Y., B. V. Gutyj, K. Y. Leskiv, I. I. Hariv, L. H. Yevtukh, and V. L. Shnaider. "Influence of cadmium on the cellular part of the immune system of young cattle." Ukrainian Journal of Veterinary and Agricultural Sciences 3, no. 2 (2020): 47–52. http://dx.doi.org/10.32718/ujvas3-2.08.
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The article presents the results of research on the effect of cadmium load on the body of young cattle. The negative effect of Cadmium on the liver causes a wide range of pathological changes at different levels of its organization. The impact of heavy metals on the immune system is particularly significant, because it performs a leading role in maintaining health and is recognized as one of the most sensitive to adverse factors, even in relatively low concentrations. The aim of the study was to investigate the influence of cadmium on the cellular part of the immune system of young cattle. The research was carried out on 10 bulls of six months of age, Ukrainian black-and-white dairy breed, which were formed into 2 groups of 5 animals each: control and experimental. The bulls of the control group were on a normal diet. Animals of the experimental group were administered cadmium chloride at a dose of 0.04 mg/kg body weight of the animal. It was found that feeding bulls with a diet of cadmium chloride, the number of B-lymphocytes on the 5th day of the experiment was 17.54 ± 0.95 %. The lowest number of B-lymphocytes was on the 20th day of testing – 15.12 ± 0.37 %. The study of the number of T-lymphocytes shows that at the beginning of analysis the number of T-lymphocytes in the blood of animals of the control and experimental groups ranged from 40.70 ± 3.62 and 40.85 ± 2.54 %. Subsequently, the number of T-lymphocytes in the blood of the experimental group began to decline. The lowest number of T-lymphocytes was in the experimental group of animals on the 20th day of the research, compared with the control group, this figure decreased by 3.63 %. The state of immunity of animals under cadmium load significantly depends on the ratio of T-helpers to T-suppressors. It was found that the immunoregulatory index of blood of bulls of the experimental group probably decreased from 10 days of the research. The number of T-helper lymphocytes in the blood of the experimental group of bulls under cadmium loading is probably reduced by 15, 20 and 30 days of testing. A probable increase in the number of T-suppressors was noted in the bull's blood of the experimental group on the 20th day of the research. Immunoglobulins of different classes are crucial among bull’s serum proteins under cadmium loading. The concentration of immunoglobulins in the blood under cadmium load decreased by 15.9 % relative to the control group on the 20th day of analysis.
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Masiuk, D. M., A. V. Kokariev, T. O. Vasilenko, and K. O. Krutii. "The formation of colostral immunity and its duration in calves during the first months of life." Ukrainian Journal of Veterinary and Agricultural Sciences 2, no. 1 (2019): 3–6. http://dx.doi.org/10.32718/ujvas2-1.01.
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The paper presents the results of a study on the formation of colostral immunity and the determination of its duration during the first months of life of calves according to the indications of the level of total IgG and antigen-specific antibodies to pathogens of рarainfluenza 3, respiratory syncytial infection and viral diarrhea of cattle. It has been established that blood serum of newborn calves does not contain antigen-specific immunoglobulins of class G to pathogens of viral diarrhea, respiratory syncytial infection and parainfluenza-3, and the level of total IgG is 2.2 ± 0.61 g/dm3. The concentration of total IgG is increased to 50.50 ± 6.33 g/dm3, and decreases to 27.25 ± 5.82 g/dm3 for 28 days, which contributes to the formation of the immunosuppressive state at the 7th day of a calf life. Starting from 35th day, there is an increase in the concentration of total IgG due to seroconverting of its own antibodies by the body of calves. Immunization of cows-mothers against viral diarrhea, respiratory syncytial infection and parainfluenza-3 contributes to the formation of antigen-specific colostral immunity in calves in the first days of life, which is preserved in diagnostic titers during 63 days after birth.
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Brooks, Roxann S., Myra T. Blanchard, Mark L. Anderson, Mark R. Hall, and Jeffery L. Stott. "Quantitative duplex TaqMan real-time polymerase chain reaction for the assessment of the etiologic agent of epizootic bovine abortion." Journal of Veterinary Diagnostic Investigation 23, no. 6 (2011): 1153–59. http://dx.doi.org/10.1177/1040638711425573.
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Epizootic bovine abortion (EBA), also commonly known as “foothill abortion,” is a late-term abortion primarily in beef cattle with significant economic impacts in California, Nevada, and Oregon. The causative agent is a novel deltaproteobacterium (aoEBA) closely related to the order Myxococcales and vectored by the soft-shelled tick Ornithodoros coriaceus. Historically, diagnosis has relied upon the pathologic examination of the fetus and the presence of elevated fetal serum immunoglobulins. Identification of the etiologic agent, a unique deltaproteobacterium, permitted the development of a quantitative duplex real-time polymerase chain reaction (qPCR) using a unique 90-bp sequence of aoEBA 16S ribosomal RNA gene in conjunction with an 88-bp sequence of the bovine β-actin gene. Reaction efficiencies were 100.9% for the 16S aoEBA gene and 93.1% for the bovine β-actin gene. Application of the duplex TaqMan to a set of aoEBA-infected fetal bovine necropsy tissues demonstrated the assay to be robust in quantitatively identifying the aoEBA bacteria and establishing host-tissue pathogen load. Consistent with previously reported immunohistochemical data, organized lymphoid tissue generally carried the heaviest bacterial load as compared to non-lymphoid tissue. The newly developed duplex TaqMan assay will facilitate diagnosis in difficult cases and provide an invaluable tool for delineating the pathogenesis of EBA.
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Koreyba, L. V. "Prediction of birth and postpartum pathology in deep-calving heifers by biochemical parameters of blood." Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 23, no. 101 (2021): 21–25. http://dx.doi.org/10.32718/nvlvet10104.
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The success of the control of obstetric diseases in cattle depends on the timely implementation of preventive measures, and, in cases of pathology, on diagnosis, effective obstetric care and treatment. A prominent place is given to blood tests among the methods that allow to objectively assess the state of health and the course of the pathological process in animals' bodies. The study of biochemical parameters of blood allows for the assessment of the state of metabolic processes in animals, with high accuracy in establishing the body's general condition and predicting complications and adjusting their prevention and therapy. The goal of the work: our work aimed to study the biochemical parameters of the blood of deep-calving heifers for the prediction, correction, and prevention of obstetric pathology during childbirth and puerperium. The research was carried out on deep-calving heifers of Holstein breed selected on the principle of analogues. Biochemical examination of blood serum was performed in the district laboratory of veterinary medicine. Indicators of protein and mineral metabolism were determined in the blood serum of deep- calving heifers: total protein, carotene, reserve alkalinity, inorganic phosphorus, albumin content, class M immunoglobulins. The obtained research results indicate that the total protein content corresponds to the norm in deep-calving heifers of the Holstein black-spotted breed. The protein coefficient exceeds the standard. There are pronounced deviations in the blood's biochemical parameters: low levels of carotene almost two-fold, reserve alkalinity – 1.91-fold, the total calcium content – by 16.08 % and the ratio of calcium to phosphorus by 17.78 %. Since the decrease in carotene content correlates with the predisposition to endometritis, the Holstein black-spotted breed's deep-calving heifers are prone to developing birth and postpartum pathology. For pregnant cows which are predisposed to postpartum obstetric pathology, that occur in weak parturition activity, failure to release the placenta, uterus subinvolution, endometritis and postpartum hypocalcaemia, unfavorable prognostication is the low concentration in blood plasma of general calcium, inorganic phosphorus and carotene.
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Topuria, Larisa, Gocha Topuria, and Victor Pirogov. "Effect of phytobiotic - Germivit on the functional state of cattle." Revista Facultad Nacional de Agronomía Medellín 74, no. 2 (2021). http://dx.doi.org/10.15446/rfnam.v74n2.85369.
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Plant-based feed additives, also known as phytobiotics, show great promise to compensate deficiencies of important biologically active substances in the diet. Therefore, this study aimed to evaluate the effect of Germivit on the feed supplement of cattle of different ages. In the first experiment, four groups of 9-month-old Simmental calves (10 animals in each group) were fed with Germivit at 0.0, 0.5, 0.7, and 0.9 g kg-1 live body weight, respectively. In the second experiment, three groups of pregnant cows (10 animals in each group) received Germivit at 0.0, 0.25, and 0.50 kg-1 live body weight, respectively. The morphological composition of the collected blood was studied using a PCE-90vet automatic hematology analyzer. The blood biochemical composition was studied using a Stat Fax 1904 biochemical analyzer, and the immunological status of the animals was evaluated using generally accepted methods in veterinary medicine: immunocompetent cells by spontaneous rosette formation; immunoglobulins by the radial immunodiffusion method; phagocytosis by using S. aureus culture; serum lysozyme activity by the photoelectrocolorimetric method; and bactericidal activity of serum by determining the degree of growth inhibition of the mixture of daily culture of E. coli in a nutritive culture broth. Germivit contributed to the improvement of the biochemical and immunological parameters in the calves. In the cows, an increase in the morphological parameters of blood was observed, their immune status improved, and their calves were born with high rates of natural resistance and health. Germivit had a positive effect on the functional state of cattle.
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Иванов, О. В., Д. Ю. Костерин, and Л. Э. Мельникова. "Development of a Set of Preventive Measures for Associative Infections of Newborn Calves." Vestnik APK Verhnevolzh`ia, no. 1(49) (March 30, 2020). http://dx.doi.org/10.35694/yarcx.2020.49.1.009.
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Представлены результаты исследования этиологической структуры инфекционных болезней молодняка в ранний период в одном из предприятий Владимирской области. Возникновению данных заболеваний способствуют скученное содержание, несоблюдение ветеринарно-санитарных требований, недоброкачественные корма и неудовлетворительное кормление коров-матерей, несвоевременное выпаивание молозива телятам, выпаивание молозива с низким содержанием иммуноглобулинов, невыполнение своевременных вакцинаций, что отрицательно сказывается на формировании иммунитета и сохранности молодняка. Авторами разработана и апробирована эффективная схема профилактических мероприятий против ассоциативных инфекций новорождённых телят в этом хозяйстве. Она охватывает период от момента осеменения коров до их содержания в сухостойный период и достижения телятами трёхнедельного возраста и включает: вакцинацию коров Кэтлмастер Голд FP5 L5 двукратно за 5 и 2 недели до осеменения для профилактики инфекционного ринотрахеита, вирусной диареи, парагриппа-3, респираторно-синтициальной инфекции и лептоспироза крупного рогатого скота; вакцинацию глубокостельных коров в сухостойный период инактивированной эмульгированной вакциной «Ротавек Корона» против ротавирусной, коронавирусной инфекции и эшерихиоза крупного рогатого скота; создание «банка молозива» от здоровых коров 3-й и более лактаций; контрольные исследования сыворотки крови телят суточного возраста для определения количества иммуноглобулинов крови, с целью коррекции уровня колострального иммунитета с помощью специфических препаратов; контроль за рождением молодняка, организация профилактических мероприятий новорождённых животных против инфекционных заболеваний; контроль качества ветеринарно-санитарной обработки и дезинфекции сосковых поилок, посуды, инвентаря, клеток для содержания телят и индивидуальных домиков. С помощью предложенной схемы удалось снизить падёж телят в хозяйстве в 2,66 раза. The results of the research of the etiological structure of infectious diseases of young animals in the early period in one of the enterprises of the Vladimir region are presented. The rapid emergence of these diseases is facilitated by stocking density, nonobservance with veterinary and sanitary requirements, low grade feed and poor feeding of mother cows, untimely calf rearing with colostrum, feeding colostrum with a low content of immunoglobulins, failure to fulfil timely vaccinations which negatively affects the formation of immunity and livability of young stock. The authors have developed and tested an effective scheme of preventive measures against associative infections of newborn calves on this farm. It covers the period from the moment of insemination of cows to their management in the dry period and reaching calves of three weeks of age and includes: vaccination of cattle CattleMaster Gold FP5 L5 twice for 5 and 2 weeks before insemination for the prevention of infectious rhinotracheitis, virus diarrhoea, parainfluenza-3, respiratory syncytial cattle infections and leptospirosis; vaccination of down calver cows during the dry period with the inactivate emulsified vaccine "Rotavek Korona" against rotavirus, coronavirus infection and colibacillosis of cattle; creation of a "colostrum bank" from healthy cows of the 3rd and more lactations; control studies of the blood serum of daily calves to determine the number of blood immunoglobulins, with the aim of correcting the level of colostral immunity using specific drugs; birth control of young animals, organization of preventive measures for newborn animals against infectious diseases; quality control of veterinary and sanitary processing and disinfection of feeding bottle, dishes, stock, cages for keeping calves and individual houses. Using the proposed scheme, it was possible to reduce the calf mortality rate on the farm by 2.66 times.