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Journal articles on the topic 'CcdA antitoxin'

Author: Grafiati

Published: 6 September 2023

Last updated: 31 July 2025

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1

Aguirre-Ramírez, Marisela, Jesús Ramírez-Santos, Laurence Van Melderen, and M. Carmen Gómez-Eichelmann. "Expression of the F plasmid ccd toxin–antitoxin system in Escherichia coli cells under nutritional stress." Canadian Journal of Microbiology 52, no. 1 (2006): 24–30. http://dx.doi.org/10.1139/w05-107.

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The ccd system of the F plasmid encodes CcdB, a protein toxic to DNA-gyrase, and CcdA, its antitoxin. The function attributed to this system is to contribute to plasmid stability by killing bacteria that lose the plasmid during cell division. However, the function of ccd in resting bacteria is not clear. Results presented show that ccd transcription increases as bacteria enter stationary phase and that the amount of the Ccd proteins is higher in bacteria under nutritional stress than in growing bacteria. Moreover, an increase in the frequency of Lac+ "adaptive" mutations was observed in statio

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2

Burger, Virginia M., Alexandra Vandervelde, Jelle Hendrix, et al. "Hidden States within Disordered Regions of the CcdA Antitoxin Protein." Journal of the American Chemical Society 139, no. 7 (2017): 2693–701. http://dx.doi.org/10.1021/jacs.6b11450.

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3

Madl, Tobias, Laurence Van Melderen, Natacha Mine, et al. "Structural Basis for Nucleic Acid and Toxin Recognition of the Bacterial Antitoxin CcdA." Journal of Molecular Biology 364, no. 2 (2006): 170–85. http://dx.doi.org/10.1016/j.jmb.2006.08.082.

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4

Lepka, Daniela, Tobias Kerrinnes, Evelyn Skiebe, Birgitt Hahn, Angelika Fruth, and Gottfried Wilharm. "Adding toYersinia enterocoliticaGene Pool Diversity: Two Cryptic Plasmids from a Biotype 1A Isolate." Journal of Biomedicine and Biotechnology 2009 (2009): 1–10. http://dx.doi.org/10.1155/2009/398434.

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We report the nucleotide sequence of two novel cryptic plasmids (4357 and 14 662 base pairs) carried by aYersinia enterocoliticabiotype 1A strain isolated from pork. As distinguished from most biotype 1A strains, this isolate, designated 07-04449, exhibited adherence to eukaryotic cells. The smaller plasmid pYe4449-1 carries five attributable open reading frames (ORFs) encoding the first CcdA/CcdB-like antitoxin/toxin system described for aYersiniaplasmid, a RepA-like replication initiation protein, and mobilizing factors MobA and MobC. The deduced amino acid sequences showed highest similarit

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5

Kwak, Yee Gyung, George A. Jacoby, and David C. Hooper. "Effect of Qnr on Plasmid Gyrase Toxins CcdB and ParE." Antimicrobial Agents and Chemotherapy 59, no. 8 (2015): 5078–79. http://dx.doi.org/10.1128/aac.00524-15.

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ABSTRACTPlasmid toxins CcdB and ParE are part of addiction systems promoting plasmid maintenance. Both target host DNA gyrase, as do quinolones and plasmid-determined Qnr proteins that protect gyrase from quinolone inhibition. We clonedqnrB4,qnrS1,ccdB,parE, and the antitoxin-encoding genesccdAandparDon compatible plasmids and tested them in combination. CcdB and ParE had no specific effect on quinolone susceptibility or Qnr protection, and Qnr did not act as a CcdB or ParE antitoxin.

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6

Cotrim, Camila Aparecida, Saulo Santesso Garrido, Eliane Trovatti, and Reinaldo Marchetto. "Síntese, caracterização e estudos de interação de um análogo da antitoxina CcdA empregando fluorescência no estado estacionário." Química Nova 33, no. 4 (2010): 841–45. http://dx.doi.org/10.1590/s0100-40422010000400014.

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7

Wu, Alma Y., Muhammad Kamruzzaman, and Jonathan R. Iredell. "Specialised functions of two common plasmid mediated toxin-antitoxin systems, ccdAB and pemIK, in Enterobacteriaceae." PLOS ONE 15, no. 6 (2020): e0230652. http://dx.doi.org/10.1371/journal.pone.0230652.

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8

Isaeva, A. S., E. E. Kulikov, N. V. Ravin, B. D. Dorokhov, K. K. Tarasyan, and A. V. Letarov. "Application of the new ccdAB-type natural toxin-antitoxin module for stabilization of inheritance of expressive plasmid vectors based on the bacteriophage N15 replicon in Escherichia coli cells." Microbiology 79, no. 5 (2010): 638–45. http://dx.doi.org/10.1134/s0026261710050085.

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9

Hudson, Lauren K., Lisha Constantine-Renna, Linda Thomas, et al. "Genomic characterization and phylogenetic analysis of Salmonella enterica serovar Javiana." PeerJ 8 (November 20, 2020): e10256. http://dx.doi.org/10.7717/peerj.10256.

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Salmonella enterica serovar Javiana is the fourth most reported serovar of laboratory-confirmed human Salmonella infections in the U.S. and in Tennessee (TN). Although Salmonella ser. Javiana is a common cause of human infection, the majority of cases are sporadic in nature rather than outbreak-associated. To better understand Salmonella ser. Javiana microbial population structure in TN, we completed a phylogenetic analysis of 111 Salmonella ser. Javiana clinical isolates from TN collected from Jan. 2017 to Oct. 2018. We identified mobile genetic elements and genes known to confer antibiotic r

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10

Yang, Chenguang, Zhenhua Fan, Lvming Wu, Bo Fu, and Hongbin Sun. "Purification and characterization of CcdB and CcdA toxin-antitoxin system from Acetobacter malorum." Bioscience, Biotechnology, and Biochemistry, April 26, 2025. https://doi.org/10.1093/bbb/zbaf063.

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Abstract The Toxin-Antitoxin (TA) system, a genetic element in microorganisms, consists of a stable toxin and an unstable antitoxin. The CcdAB system, a typical TA system, encodes the CcdB toxin and CcdA antitoxin and was identified in Acetobacter, though its biological role remains unclear. In this study, CcdA and CcdB proteins were successfully expressed, and purification conditions were optimized to obtain high-purity proteins. Their interaction was studied using a pull-down assay and confirmed through bioinformatics tools, revealing stable secondary structures. Induced expression of CcdB i

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11

Chandra, Soumyanetra, Kritika Gupta, Shruti Khare, et al. "The high mutational sensitivity of ccdA antitoxin is linked to codon optimality." Molecular Biology and Evolution, September 7, 2022. http://dx.doi.org/10.1093/molbev/msac187.

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Abstract Deep mutational scanning studies suggest that synonymous mutations are typically silent and that most exposed, non active-site residues are tolerant to mutations. Here we show that the ccdA antitoxin component of the E.coli ccdAB toxin-antitoxin system is unusually sensitive to mutations when studied in the operonic context. A large fraction (∼80%) of single-codon mutations, including many synonymous mutations in the ccdA gene shows inactive phenotype, but they retain native-like binding affinity towards cognate toxin, CcdB. Therefore, the observed phenotypic effects are largely not d

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12

McCuaig, Frazer, Jody Winter, Jonathan Thomas, Gareth McVicker, and Lesley Hoyles. "Genotypic and phenotypic characterisation of the Klebsiella oxytoca complex." Access Microbiology 2, no. 7A (2020). http://dx.doi.org/10.1099/acmi.ac2020.po0163.

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Klebsiella spp. are associated with 3 to 7% of nosocomial infections and can be responsible for a range of conditions including pneumonia, bloodstream infections, meningitis, and necrotizing enterocolitis in infants. The role of Klebsiella pneumoniae in causing disease is well-characterised but, to date, the closely related species Klebsiella oxytoca has not received the same attention, despite often encoding extended-spectrum beta-lactamases and carbapenemases in clinical settings. K. oxytoca is the causative agent of Clostridiodes difficile-negative antibiotic-associated haemorrhagic colitis

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13

Ba, Fang, Yufei Zhang, Luyao Wang, Wan‐Qiu Liu, and Jian Li. "Blue‐Purple evaluation: Chromoproteins facilitate the identification of BioBrick compatibility." Biotechnology and Bioengineering, October 14, 2024. http://dx.doi.org/10.1002/bit.28862.

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AbstractSynthetic BioBricks introduce novel capabilities to manipulate genetic information, direct transcription‐translation processes, and program cellular behaviors in living organisms. To maintain the stability and functionality of synthetic BioBricks, assembled DNA fragments should be mutually compatible without inducing negative effects such as metabolic burden or cellular toxicity in host cells. However, a simple, rapid, and reliable method to evaluate BioBrick compatibility remains to be developed. In this study, we report BP (Blue/Purple, Ban/Pick) evaluation, a method utilizing chromo

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14

Zavrtanik, Uroš, San Hadži, and Jurij Lah. "Unraveling the Thermodynamics of Ultra-tight Binding of Intrinsically Disordered Proteins." Frontiers in Molecular Biosciences 8 (August 31, 2021). http://dx.doi.org/10.3389/fmolb.2021.726824.

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Protein interactions mediated by the intrinsically disordered proteins (IDPs) are generally associated with lower affinities compared to those between globular proteins. Here, we characterize the association between the intrinsically disordered HigA2 antitoxin and its globular target HigB2 toxin from Vibrio cholerae using competition ITC experiments. We demonstrate that this interaction reaches one of the highest affinities reported for IDP-target systems (KD = 3 pM) and can be entirely attributed to a short, 20-residue-long interaction motif that folds into α-helix upon binding. We perform an

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15

Nanda, Bahnikana, Jayantika Bhowmick, Raghavan Varadarajan, and Siddhartha P. Sarma. "Backbone assignment of CcdB_G100T toxin from E.coli in complex with the toxin binding C-terminal domain of its cognate antitoxin CcdA." Biomolecular NMR Assignments, September 14, 2024. http://dx.doi.org/10.1007/s12104-024-10201-6.

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16

Zhang, He, Shuan Tao, Huimin Chen, et al. "The biological function of the type II toxin-antitoxin system ccdAB in recurrent urinary tract infections." Frontiers in Microbiology 15 (April 16, 2024). http://dx.doi.org/10.3389/fmicb.2024.1379625.

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Urinary tract infections (UTIs) represent a significant challenge in clinical practice, with recurrent forms (rUTIs) posing a continual threat to patient health. Escherichia coli (E. coli) is the primary culprit in a vast majority of UTIs, both community-acquired and hospital-acquired, underscoring its clinical importance. Among different mediators of pathogenesis, toxin-antitoxin (TA) systems are emerging as the most prominent. The type II TA system, prevalent in prokaryotes, emerges as a critical player in stress response, biofilm formation, and cell dormancy. ccdAB, the first identified typ

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17

Gupta, Kritika, Arti Tripathi, Alishan Sahu, and Raghavan Varadarajan. "Contribution of the Chromosomal ccdAB Operon to Bacterial Drug Tolerance." Journal of Bacteriology 199, no. 19 (2017). http://dx.doi.org/10.1128/jb.00397-17.

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ABSTRACT One of the first identified and best-studied toxin-antitoxin (TA) systems in Escherichia coli is the F-plasmid-based CcdAB system. This system is involved in plasmid maintenance through postsegregational killing. More recently, ccdAB homologs have been found on the chromosome, including in pathogenic strains of E. coli and other bacteria. However, the functional role of chromosomal ccdAB genes, if any, has remained unclear. We show that both the native ccd operon of the E. coli O157 strain (ccd O157) and the ccd operon from the F plasmid (ccd F), when inserted on the E. coli chromosom

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18

Bajaj, Priyanka, Munmun Bhasin, and Raghavan Varadarajan. "Molecular bases for strong phenotypic effects of single synonymous codon substitutions in the E. coli ccdB toxin gene." BMC Genomics 24, no. 1 (2023). http://dx.doi.org/10.1186/s12864-023-09817-0.

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Abstract Background Single synonymous codon mutations typically have only minor or no effects on gene function. Here, we estimate the effects on cell growth of ~ 200 single synonymous codon mutations in an operonic context by mutating almost all positions of ccdB, the 101-residue long cytotoxin of the ccdAB Toxin-Antitoxin (TA) operon to most degenerate codons. Phenotypes were assayed by transforming the mutant library into CcdB sensitive and resistant E. coli strains, isolating plasmid pools, and subjecting them to deep sequencing. Since autoregulation is a hallmark of TA operons, phenotypes

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19

Al Marjania, Mohammed F., Ebrahim Kouhsari, Fatima S. Ali, and Sawsan H. Authman. "Evaluation of type II toxin-antitoxin systems, antibiotic resistance profiles, and biofilm quorum sensing genes in Acinetobacter baumannii isolates in Iraq." Infectious Disorders - Drug Targets 20 (May 25, 2020). http://dx.doi.org/10.2174/1871526520666200525170318.

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Background: Bacterial Toxin-Antitoxin (TAs) systems are extensive two-component elements in the bacterial genome, which involved in many key biological functions including growth arrest, survival, biofilm formation, plasmid maintenance, defense against phages, persistence and virulence. Aim: This study aimed to assess the molecular determinants involved in TAs, biofilm quorum sensing, and antibiotic resistance profiles in Acinetobacter baumannii isolated from Baghdad`s hospitals in Iraq. Methods: A total of 127 A. baumannii isolates were collected from 2160 different clinical samples. The anti

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20

Roshani, Mahdaneh, Mohammad Taheri, Alireza Goodarzi, Rassoul Yosefimashouf, and Leili Shokoohizadeh. "Evaluation of antibiotic resistance, toxin-antitoxin systems, virulence factors, biofilm-forming strength and genetic linkage of Escherichia coli strains isolated from bloodstream infections of leukemia patients." BMC Microbiology 23, no. 1 (2023). http://dx.doi.org/10.1186/s12866-023-03081-8.

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Abstract Background One of the most common complications in patients with febrile neutropenia, lymphoma, leukemia, and multiple myeloma is a bloodstream infection (BSI). Objective This study aimed to evaluate the antibiotic resistance patterns, virulence factors, biofilm-forming strength, and genetic linkage of Escherichia coli strains isolated from bloodstream infections (BSIs) of leukemia patients. Methods The study conducted in Iran from June 2021 to December 2022, isolated 67 E. coli strains from leukemia patients’ bloodstream infections in hospitals in two different areas. Several techniq

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21

Whelan, Rachel, Martyna Cyganek, Charlotte L. Oxley, Benjamin Dickins, Jonathan C. Thomas, and Gareth McVicker. "Genetic and phenotypic analysis of the virulence plasmid of a non-Shigatoxigenic enteroaggregative Escherichia coli O104:H4 outbreak strain." Microbiology 171, no. 3 (2025). https://doi.org/10.1099/mic.0.001550.

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Enteroaggregative Escherichia coli O104:H4 is best known for causing a worldwide outbreak in 2011 due to the acquisition of a Shiga-like toxin alongside traditional enteroaggregative virulence traits; however, whilst the 2011 outbreak strain has been well studied, the virulence plasmid of O104:H4 has been subjected to far less experimental analysis. In this paper, we analyse the genetic and phenotypic contribution of the pAA virulence plasmid to a non-Shigatoxigenic O104:H4 strain (1070/13) that was nonetheless implicated in a substantial UK outbreak in 2013. We find that pAA1070 is 99.95% ide

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22

Fan, Lin-Ping, Yang Yu, Shanshan Huang, et al. "Genetic characterization and passage instability of a novel hybrid virulence plasmid in a ST23 hypervirulent Klebsiella pneumoniae." Frontiers in Cellular and Infection Microbiology 12 (July 28, 2022). http://dx.doi.org/10.3389/fcimb.2022.870779.

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Hypervirulent variants of Klebsiella pnuemoniae (hvKP), which causes life-threatening infections, is a global priority pathogen and frequently harbours virulence plasmids. The virulence plasmids have emerged as the predominant vehicles carrying the major pathogenic determinants of hypermucoviscosity and hypervirulence phenotypes. In the present study, we characterized a novel virulence plasmid in AP8555, an ST23 hvKP strain, which induced a metastatic infection and fatal septic shock in a critically ill patient. The serum killing assay, the quantitative biofilm formation assay, the G.mellonell

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23

Chaudhary, Shobhi, Waseem Ali, Mohit Yadav, et al. "Computational exploration of the genomic assignments, molecular structure, and dynamics of the ccdAB ^Xn ² toxin-antitoxin homolog with its bacterial target, the DNA gyrase, in the entomopathogen Xenorhabdus nematophila." Journal of Biomolecular Structure and Dynamics, February 7, 2024, 1–15. http://dx.doi.org/10.1080/07391102.2024.2311337.

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