Academic literature on the topic 'CDNA library construction'

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Journal articles on the topic "CDNA library construction"

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Fedchenko, V. I., A. A. Kaloshin, and A. E. Medvedev. "A novel vector for construction of cDNA library." Biomeditsinskaya Khimiya 56, no. 3 (2010): 329–41. http://dx.doi.org/10.18097/pbmc20105603329.

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A new original vector pEM-(dT)40(f+) has been prepared. It can be used for cDNA library construction from polyadenylated mRNA, isolated from various sources. The pGEM-(dT)40f(+) is initially transformed into single stranded and then into a linear form and its (dT)40 tail at 3'-end is used as the vector-primer for synthesis of the first strand cDNA. The use of a synthetic oligonucleotide complementary to the vector and recombinant DNA results in vector cyclization and synthesis of the second strand cDNA. This approach significantly simplifies cDNA library construction, it does not require PCR r
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Nomura, Takashi, Tokuya Takahashi, Kohji Hara, and Hiroyoshi Nohara. "Construction of a bovine gingival cDNA library." Japanese Journal of Oral Biology 33, no. 6 (1991): 560–66. http://dx.doi.org/10.2330/joralbiosci1965.33.560.

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Oh, Gyu-Dong, Eun-Jo Shim, Sang-Jin Jun, and Young-Doo Park. "Construction of cDNA Library and EST Analysis Related to Seed-hair Characteristics in Carrot." Korean Journal of Horticultural Science and Technology 31, no. 6 (December 31, 2013): 782–89. http://dx.doi.org/10.7235/hort.2013.13108.

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Wu, Wei, Yongfeng Sun, Tongao Yang, Jingtao Hu, Zhe Hao, Yujian Sui, Yingying Fu, Lu Chen, and Haiyang Zhu. "Construction of Full-Length Goose Muscle cDNA Library." Journal of Animal and Veterinary Advances 11, no. 12 (December 1, 2012): 2106–9. http://dx.doi.org/10.3923/javaa.2012.2106.2109.

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Huang, Yao, Chuanling Qiao, Jing Wang, and Naihu Wu. "CONSTRUCTION OF cDNA LIBRARY FROM INSECTICIDE-RESISTANT HOUSEFLIES." Insect Science 1, no. 2 (June 1994): 191–93. http://dx.doi.org/10.1111/j.1744-7917.1994.tb00211.x.

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Roeder, T. "Solid-phase cDNA library construction, a versatile approach." Nucleic Acids Research 26, no. 14 (July 1, 1998): 3451–52. http://dx.doi.org/10.1093/nar/26.14.3451.

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Soares, M. B., M. F. Bonaldo, P. Jelene, L. Su, L. Lawton, and A. Efstratiadis. "Construction and characterization of a normalized cDNA library." Proceedings of the National Academy of Sciences 91, no. 20 (September 27, 1994): 9228–32. http://dx.doi.org/10.1073/pnas.91.20.9228.

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Patanjali, S. R., S. Parimoo, and S. M. Weissman. "Construction of a uniform-abundance (normalized) cDNA library." Proceedings of the National Academy of Sciences 88, no. 5 (March 1, 1991): 1943–47. http://dx.doi.org/10.1073/pnas.88.5.1943.

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Sui, Zhenghong, and Klaus V. Kowallik. "Construction of cDNA library of Pyrocystis lunula (Pyrophyta)." Journal of Ocean University of China 3, no. 2 (October 2004): 141–44. http://dx.doi.org/10.1007/s11802-004-0024-9.

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Bellemare, Guy, Claude Potvin, and Diane Bergeron. "High-yield method for directional cDNA library construction." Gene 98, no. 2 (February 1991): 231–35. http://dx.doi.org/10.1016/0378-1119(91)90178-e.

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Dissertations / Theses on the topic "CDNA library construction"

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Holm, Kora. "Construction of a cDNA library for the vine mealybug, Planococcus ficus (Signoret)". Thesis, Stellenbosch : Stellenbosch University, 2008. http://hdl.handle.net/10019.1/4083.

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Thesis (MSc (Genetics))--Stellenbosch University, 2008.<br>The vine mealybug, Planococcus ficus (Signoret), is a severe pest of grapevine in many grape and wine producing countries around the world. It is renowned not only for the considerable damage it infers to grapevine of its own accord, but in particular for its role in transmitting deleterious viral diseases such as grapevine leafroll disease, Kober stem grooving, Shiraz disease and corky bark. Incidentally, it is an exceptionally tenacious antagonist of grapevine, being resistant to both chemical and biological control mechanisms.
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Holm, Kora. "Construction of a cDNA library for the vine mealybug, Planococcus ficus (Signoret) /." Link to the online version, 2008. http://hdl.handle.net/10019/1884.

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Builes, Janette Cristina. "Construction and screening of A cDNA library for the C3 gene(s) of the Nurse Shark (Ginglymostoma Cirratum)." FIU Digital Commons, 2001. http://digitalcommons.fiu.edu/etd/1940.

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Mammalian C3 is a complement protein which consists of an α chain (125kDa) and β chain (75kDa) held together by a disulfide bond. The a chain contains a conserved thiolester site which provides the molecule with opsonic properties. The protein is synthesized as a single pro-C3 molecule which is post-translationally modified. C3 genes have been identified in organisms from different phyla, however, the shark C3 gene remains to be cloned. Sequence data from the shark will contribute to understanding further the evolution of this key protein. To obtain additional sequence data for shark C3 genes
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Ponza, Pattareeya, and pattareeya pon@biotec or th. "Molecular markers of ecotoxicological interest in the rainbowfish Melanotaenia fluviatilis." RMIT University. Applied Science, 2007. http://adt.lib.rmit.edu.au/adt/public/adt-VIT20080102.121231.

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The Crimson-spotted rainbowfish (Melanotaenia fluviatilis) from the Murray-Darling basin of Australia is a common indicator species in Australian ecotoxicology. Biochemical changes have been investigated in this species, but not molecular markers of ecotoxicological interest. In this study genes of M. fluviatilis were isolated using a cDNA library and sequences analysed. Of 345 randomly selected clones, 94 shared similarity with 26 different genes in other organisms in public databases. Amongst these, reproductive genes coding for vitellogenin, retinol binding protein, sialyltransferase and zo
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Soliman, Hatem Mohamed Touhan. "Construction and Screening of an Expression cDNA Library from the Triactinomyxon Spores of Myxobolus cerebralis, the causative agent of Salmonid Whirling Diseases." Diss., lmu, 2005. http://nbn-resolving.de/urn:nbn:de:bvb:19-39819.

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Soliman, Hatem Mohamed Touhan [Verfasser], and Mansour El [Akademischer Betreuer] Matbouli. "Construction and Screening of an Expression cDNA Library from the Triactinomyxon Spores of Myxobolus cerebralis, the causative agent of Salmonid Whirling Diseases / Hatem Mohamed Touhan Soliman. Betreuer: Mansour El-Matbouli." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2005. http://d-nb.info/1108093574/34.

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Hamdi, Saïd. "Regulation genetique et biochimique de l'auxine dans les souches fertiles et steriles de mercurialis annua : construction d'une banque de cdnas specifiques de la sterilite male." Orléans, 1987. http://www.theses.fr/1987ORLE2037.

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Huang, Zhen-Ming, and 黃震銘. "Construction of trichoderma koningii G39 cDNA library." Thesis, 1987. http://ndltd.ncl.edu.tw/handle/39237178009948159017.

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Zhang, Zhi Rui, and 張芝瑞. "Construction of mung bean cDNA library and cloning of B-galactosidase cDNA." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/29515359133940517941.

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Su, Yu-liang, and 蘇育良. "Construction and analysis of high reproductive porcine oocyte cDNA library." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/23810386909378501295.

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碩士<br>國立中山大學<br>生物醫學科學研究所<br>92<br>The progress of studies on genes concerning the development and differentiation of early swine embryos have been delayed by limited paucity material. In order to identify the porcine ESTs associates with promoting its breeding efficiency, a cDNA library and ESTs database from oocytes of high reproductive swine is established. Oocytes were obtained from Duroc pig by superovulation which was performed by Taiwan Livestock Research Institute, Council of Agriculture. Total RNA was isolated from 50 mature oocytes, reverse transcription is then performed, followed b
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Book chapters on the topic "CDNA library construction"

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Schenk, Jörg A. "Two Hybrid cDNA Cloning." In Genetic Library Construction and Screening, 145–65. Berlin, Heidelberg: Springer Berlin Heidelberg, 2002. http://dx.doi.org/10.1007/978-3-642-56408-6_8.

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Curtis Bird, R., and Gin Wu. "Subtractive Hybridization and cDNA Cloning." In Genetic Library Construction and Screening, 97–125. Berlin, Heidelberg: Springer Berlin Heidelberg, 2002. http://dx.doi.org/10.1007/978-3-642-56408-6_6.

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Huang, Betty C. B., and Ying Luo. "Yeast One and Two Hybrid cDNA Cloning." In Genetic Library Construction and Screening, 167–85. Berlin, Heidelberg: Springer Berlin Heidelberg, 2002. http://dx.doi.org/10.1007/978-3-642-56408-6_9.

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Silverstein, Peter S., Shilpa J. Buch, and R. Curtis Bird. "Strategies for cDNA Cloning and Mapping RNA Transcripts." In Genetic Library Construction and Screening, 3–19. Berlin, Heidelberg: Springer Berlin Heidelberg, 2002. http://dx.doi.org/10.1007/978-3-642-56408-6_1.

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Jiang, Guifeng, and D. Jed Harrison. "mRNA Isolation for cDNA Library Construction on a Chip." In Micro Total Analysis Systems 2000, 537–40. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-017-2264-3_126.

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Snead, Marjory A., Michelle A. Alting-Mees, and Jay M. Short. "cDNA Library Construction for the Lambda ZAP®-Based Vectors." In Plant Virology Protocols, 255–67. Totowa, NJ: Humana Press, 1998. http://dx.doi.org/10.1385/0-89603-385-6:255.

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Zhu, York Y., Alex Chenchik, Roger Li, Florence Y. Hsieh, and Paul D. Siebert. "Construction of cDNA Libraries from Small Quantities of Total RNA Using Template Switching Catalyzed by M-MLV Reverse Transcriptase." In Genetic Library Construction and Screening, 69–93. Berlin, Heidelberg: Springer Berlin Heidelberg, 2002. http://dx.doi.org/10.1007/978-3-642-56408-6_5.

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Singh, Rupesh Kumar, Weina Hou, and Gregory Franklin. "Construction of Hypericin Gland-Specific cDNA Library via Suppression Subtractive Hybridization." In Methods in Molecular Biology, 317–34. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3332-7_22.

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Liew, C. C., D. M. Hwang, R. X. Wang, S. H. Ng, A. Dempsey, D. H. Y. Wen, H. Ma, et al. "Construction of a human heart cDNA library and identification of cardiovascular based genes (CVBest)." In Novel Methods in Molecular and Cellular Biochemistry of Muscle, 81–87. Boston, MA: Springer US, 1997. http://dx.doi.org/10.1007/978-1-4615-6353-2_8.

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Sahr, Tobias, and Carmen Buchrieser. "cDNA Library Construction for Next-Generation Sequencing to Determine the Transcriptional Landscape of Legionella pneumophila." In Methods in Molecular Biology, 555–66. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-161-5_34.

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Conference papers on the topic "CDNA library construction"

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Park, Sang-Eun. "cDNA library construction and gene screening ofHarmonia axyridis(Coleoptera: Coccinellidae) for gene functional analysis." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.112244.

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Kim, Jeong Hee. "cDNA library construction of 28-spotted potato ladybird,Henosepilachna vigintioctomaculata, for RNA interference application." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.110968.

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Pan, Baoping, Liyan Zhang, Weiwei Gao, and Xin Song. "Construction of cDNA Library and Preliminary Screening of Immune-Related Genes in Cyclina sinensis." In 2011 5th International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2011. http://dx.doi.org/10.1109/icbbe.2011.5780015.

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Liu, Jiao, Shaoping Fu, Yuqiang Guo, Shuo Wang, Ruijun Duan, Ruimei Li, Yuan Yao, and Jianchun Guo. "Construction of a High-Quality Yeast One/two-hybrid cDNA Library from Cassava (Manihot Esculenta Crantz)." In International Conference on Biomedical and Biological Engineering. Paris, France: Atlantis Press, 2016. http://dx.doi.org/10.2991/bbe-16.2016.70.

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Huber, P., J. Dalmon, M. Laurent, G. Courtois, D. Thevenon та G. Marguerie. "CHARACTERIZATION OFTHE 5’FLANKING REGION FOR THE HUMAN FIBRINOGEN β GENE". У XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642889.

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Fibrinogen is coded by three separate genes located in a 50kb region of chromosome 4 and organized in a α - β - γ orientation with an inversion of the gene 3- A human genomic library was constructed using the EMBL4 phage and screened with cDNA probes coding for human fibrinogen Aα, Bβ and γ chains. Clones, covering the fibrinogen locus,were identified, and their organization was analyzed by means of hybridization and restriction mapping. Among these clones one recombinant phage containing the β gene and large 5’ and 3’ -flanking sequences was isolated.To identify the regulatory sequences Dpstr
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Reports on the topic "CDNA library construction"

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Mitchell, S. C., D. Bocskai, and Y. Cao. Construction of genome-wide physical BAC contigs using mapped cDNA as probes: Toward an integrated BAC library resource for genome sequencing and analysis. Annual report, July 1995--January 1997. Office of Scientific and Technical Information (OSTI), December 1997. http://dx.doi.org/10.2172/639708.

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