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1
Tong, Weihan, Maryanne Romero, Vivien Lim, et al. "Reliability of Graders and Comparison with an Automated Algorithm for Vertical Cup-Disc Ratio Grading in Fundus Photographs." Annals of the Academy of Medicine, Singapore 48, no. 9 (2019): 282–89. http://dx.doi.org/10.47102/annals-acadmedsg.v48n9p282.
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Introduction: We aimed to investigate the intergrader and intragrader reliability of human graders and an automated algorithm for vertical cup-disc ratio (CDR) grading in colour fundus photographs. Materials and Methods: Two-hundred fundus photographs were selected from a database of 3000 photographs of patients screened at a tertiary ophthalmology referral centre. The graders included glaucoma specialists (n = 3), general ophthalmologists (n = 2), optometrists (n = 2), family physicians (n = 2) and a novel automated algorithm (AA). In total, 2 rounds of CDR grading were held for each grader on 2 different dates, with the photographs presented in random order. The CDR values were graded as 0.1-1.0 or ungradable. The grading results of the 2 senior glaucoma specialists were used as the reference benchmarks for comparison. Results: The intraclass correlation coefficient values ranged from 0.37-0.74 and 0.47-0.97 for intergrader and intragrader reliability, respectively. There was no significant correlation between the human graders’ level of reliability and their years of experience in grading CDR (P = 0.91). The area under the curve (AUC) value of the AA was 0.847 (comparable to AUC value of 0.876 for the glaucoma specialist). Bland Altman plots demonstrated that the AA’s performance was at least comparable to a glaucoma specialist. Conclusion: The results suggest that AA is comparable to and may have more consistent performance than human graders in CDR grading of fundus photographs. This may have potential application as a screening tool to help detect asymptomatic glaucoma-suspect patients in the community. Key words: Eyes, Glaucoma, Intraocular pressure
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Rupes, Ivan, Jana Jochová, and Paul G. Young. "Markers of cell polarity during and after nitrogen starvation in Schizosaccharomyces pombe." Biochemistry and Cell Biology 75, no. 6 (1997): 697–708. http://dx.doi.org/10.1139/o97-084.
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In Schizosaccharomyces pombe, nitrogen starvation induces transient acceleration of cell division and reduction in cell size with a final arrest in G1. The division size control appears to be impaired by mutations in cdr1/nim1 and cdr2, genes that encode protein kinases mediating nutritional control over the mitotic cycle. cdr- cells arrest after fewer rounds of division and are larger than the wild type. Recent work suggests that long-term nitrogen starvation causes S. pombe wild-type cells to become spherical, which suggests loss of cell polarity. cdr mutants retain the elongated shape, indicating a potential difference in cell polarity control relative to the wild type. We examined several markers related to maintenance of cell polarity in S. pombe following nitrogen starvation including cell division scar pattern and actin and microtubule cytoskeleton. Wild-type cells as well as cdr mutants maintained a normal cell division scar pattern throughout nitrogen starvation but cells dividing under these conditions developed a wall malformation in the center of the septum. In cells arrested by nitrogen starvation, actin patches, normally associated with sites of cell wall deposition, were larger and distributed randomly along the cell surface. Cytoplasmic arrays of microtubules, which are thought to be involved in control of the polarity signal, were not visibly affected. The effects were similar in wild-type cells and in cdr- mutants. Upon refeeding, the new growth always reoccurred at the tip zones and there were only small deviations of its direction from the original axis. The results indicate that cell polarity is preserved both in wild-type cells, which arrest in G1 and appear spherical, and in cdr1/nim1 and cdr2 mutants, which arrest in G2 and appear polarized throughout the starvation period. Key words: cell polarity, fission yeast, nitrogen starvation, actin, microtubules, cdr1/nim1, cdr2.
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Simonov, R. O., R. G. Yapparov, D. A. Valishin, and E. M. Gareev. "Evaluation of T cell-mediated immunity in HIV-infected patients with helminthiasis." Infekcionnye bolezni 19, no. 1 (2021): 33–38. http://dx.doi.org/10.20953/1729-9225-2021-1-33-38.
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Objective. To analyze T cell-mediated immunity (subpopulations of CD3+, CD4+, and CD8+ lymphocytes) in HIV-infected patients with helminthiasis receiving or not receiving antiretroviral therapy (ART). Patients and methods. This study included 159 individuals; 100 of them had subclinical stage 3 HIV infection and helminthiasis (caused by different helminths) and were divided into two experimental groups depending on whether they received ART or not. The control group 1 comprised 29 HIV-positive people without helminthiasis not receiving ART, whereas the control group 2 included 30 HIV-negative people with helminthiasis. Patients in the experimental groups and control group 2 were followed up during the study and received anthelmintic treatment. The assessment of the immune status using monoclonal antibodies against specific antigens of subpopulations of T-lymphocytes (CD3+, CD4+ cells, cytotoxic T-cells-suppressors-CD8+ and CD4+/CD8+ ratio) was performed in the laboratory of the Republican Center for the Prevention and Control of AIDS and Infectious Diseases, Ufa, Russian Federation. Results. In this study, we evaluated the relative count of CD3+, CD4+, and CD8+ T cells in HIV-infected patients with helminthiasis at different time-pints during 6 months. We observed significant differences in the CD3+, CD3+CD4+, and CD3+CD8+ cell count between HIV-infected patients with helminthiasis on ART and without ART. Patients in the experimental group on ART demonstrated a significantly lower CD3+ cell count compared to patients in the experimental group not on ART (2.3 times lower; p < 0.01), as well as lower CD3+CD4+ cell count (1.5 times lower; p < 0.05) and CD4+CD8+ cell count (1.9 times lower; p < 0.05). Our findings suggest that HIV-infected people with helminthiasis on ART are more likely to have their CD3+, CD4+, and CD8+ Т-cell count normalized than those not receiving ART. Conclusion. The assessment of the immune status (T-cell medicated immunity) in the study groups demonstrated that HIVinfected patients with helminthiasis on ART presented with a gradual increase of the relative CD3+ cell count throughout the study (62.5 ± 5.6 %), whereas HIV-infected patients with helminthiasis receiving no ART presented with a gradual decrease of the relative CD3+ cell count (28.0 ± 4%). HIV-infected patients without helminthiasis and receiving no ART (control group 1) also demonstrated a decrease of the relative CD3+ cell count (28.1 ± 3.5%). We also observed a clear trend towards the normalization of relative CD3+CD4+ T-cell count (41.4 ± 8.2%) in HIV-infected patients with helminthiasis on ART, while patients from other groups (including HIV-infected patients with helminthiasis without ART and individuals in both control groups) demonstrated a tendency to a steady decline in the relative CD3+CD4+ cell count at all time-points. We also found that HIV-infected patients with helminthiasis on ART had their CD4+/CD8+ ratio back to almost normal by month 6 (45.7 ± 3.7%), whereas HIV-infected patients with helminthiasis without ART and patients from the control group 1 had their mean CD4+CD8+ ratio gradually decreasing throughout the study (27.5 ± 4.9% and 30.5 ± 7.1% respectively). The parameters of T-cell mediated immunity (subpopulations of CD3+, CD4+, and CD8+ lymphocytes) showed a more pronounced tendency to normalization in HIV-infected patients with helminthiasis who received ART. Our findings suggest that in HIV-infected patients with helminthiasis on ART, compensatory mechanisms of T-lymphocytes predominate, in contrast to HIV-infected patients with helminthiasis receiving no ART and the control group of HIV-infected patients receiving no ART, in whom we observed immunodeficiency of different grades. Key words: HIV infection, helminthiasis, T-cell immunity
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Tumanova, L. E., and E. V. Kolomiiets. "Phenotypic features of immunocompetent cells in I-II pregnancy trimesters in women with anamnese infertility." UKRAINIAN JOURNAL OF PERINATOLOGY AND PEDIATRICS, no. 1(89) (March 29, 2022): 11–16. http://dx.doi.org/10.15574/pp.2022.89.11.
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It is impossible to solve the socio-economic problem of increasing the birth rate in Ukraine without determining the mechanisms of pregnancy disorders, among which immune ones occupy an important place, and developing individualized scientifically based therapy schemes. Purpose - to determine the characteristics of levels of immunocompetent blood cells based on the CD phenotype in women with a history of infertility at different stages of pregnancy. Materials and methods. With a FACSCan cell cytofloorimeter (Becton Dickinson, USA) and a test of Becton Dickinson systems (USA) determined using monoclonal antibodies (MCAs) to differentiated lymphocyte antigens, and activation markers (HLA-DR, CD25, CD69) or having inhibitory properties (CD158a) relative levels of immunocompetent cells 436 non-pregnant (reference group n) and 514 pregnant women with a history of infertility at different dates of the I and II trimesters of pregnancy (groups a-e). Results. Against the background of reduced lymphocyte count and increased blood granulocytes in pregnant women, a significantly high relative level of T-L and T-cytotoxic subpopulations were found with their activation (CD3+CD8+HLA-DR+- and CD3+CD8+Cd In this gestation period there is also an increase as the number of T-helpers (CD3+CD4+-L), and their activation with expression on the membranes of HLA-DR- and CD25-molecules. In the first 10 weeks of pregnancy, a decrease in the expression of inhibitory molecules CD158a (KIR2DL1) on NK T CD3+CD4+CD56+-cl and the relative level of B-lymphocytes (CD19+), as well as their subpopulations B-1a (CD19+CD5+) from 8 to 28 weeks was noted. Conclusions. The peculiarities of the levels of peripheral blood immune system cells according to their CD phenotypes make it possible to observe the dynamics of the most important of them (CD3+CD8+HLA-DR+-, CD3+CD8+CD56+-, CD. The research was carried out in accordance with the principles of the Helsinki declaration. The study protocol was approved by the Local ethics committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors. Key words: CD-phenotype, immunocompetent cells, pregnant women, history of infertility, flow cytometry.
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Milasheuski, Y. V., and T. A. Imshanetskaya. "Micropulse transscleral cyclophotocoagulation: clinical outcomes of treatment of early stages open-angle glaucoma." Russian ophthalmology of children, no. 2 (June 20, 2021): 36–41. http://dx.doi.org/10.25276/2307-6658-2021-2-36-41.
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Purpose. To evaluate treatment efficiency of open-angle glaucoma with micropulse transscleral cyclophotocoagulation, to determine the optimal total energy of laser exposure and to assess the stability of the glaucoma process after treatment. Material and methods. A prospective study was carried out. It included 34 (21 patients) with primary open-angle and pseudoexfoliative glaucoma of stages 1-3, which undergone micropulse transscleral cyclophotocoagulation. The patients were divided into subgroups 1 and 2, depending on the total amount of laser energy (100 J and 150 J, respectively). At different periods of observation, the intraocular pressure, best corrected visual acuity, visual fields, cup-to-disc ratio and the number of antiglaucoma drugs were determined. Results. In the general group, all operated patients achieved a decrease in IOP to a tolerant level, from a median value of 27 [25; 28] in the preoperative period, up to 17.5 [15; 20] at the end of the observation period. The number of used drugs decreased statistically significantly, from 3.0 [3.0; 3.0] to 2.0 [1.0; 2.0]. There was a statistically significant increase in the mean deviation of the retinal photosensitivity, from –4.85 [–8.25; –2.17] to –4.36 [–7.06; –2.37] after 18 months. Indicators of best corrected visual acuity and CDR remained stable throughout the observation period. In subgroup 2 (150J), a statistically more significant decrease in IOP level (p=0.033) and the number of used drugs (p=0.001) was achieved compared with subgroup 1 (100J). Conclusion. Micropulse transscleral cyclophotocoagulation is an effective and safe method of treating open-angle glaucoma in patients with early stages of the disease and high values of best corrected visual acuity. Using a total amount of energy in 150J contributes to a more pronounced decrease in IOP and a decrease in the number of applied drugs than an energy in 100J. Key words: open-angle glaucoma, pseudoexfoliative glaucoma, micropulse cyclophotocoagulation.
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Li, Y. Z., R. J. Christopherson, and C. J. Field. "Effects of beta-adrenergic receptor agonist and low environmental temperature on the immune system of growing lambs." Canadian Journal of Animal Science 80, no. 4 (2000): 605–13. http://dx.doi.org/10.4141/a99-106.
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Sixteen lambs were studied to examine the effects of cold environment and a beta-agonist on their immune status. The beta-agonist, L-646, 969, was fed for 4 wk at 0.28 or 0 mg kg−0.75 BW to animals housed in a warm (20 °C) or a moderately cold (0 °C) environment. The cold environment suppressed PWM-stimulated proliferation at week 3 (P < 0.03) and week 4 (P < 0.01). The percentage of CD4 cells and CD4:CD8 cell ratio were decreased at week 1 (P < 0.02; P < 0.05) and week 3 (P < 0.001; P < 0.003), and the percentage of CD2 cells (P < 0.05) was decreased at week 2 at the low-temperature. The beta-agonist did not influence leukocyte profile and lymphocyte functions. The results suggest that a cold environment may influence immune function by suppressing lymphocytes expressing CD2, CD4 and CD4:CD8 cell ratio, and changing lymphocyte proliferative response to a T cell mitogen. Further investigation is required to identify the effects of prolonged and intense cold exposure on the immune system with a large number of animals and the implications of current observations for the health of animals in a cold environment. Key words: Immunity, cold environment, beta-agonist, lambs
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Zvarych, L. M., D. A. Bazyka, I. M. Ilienko, et al. "STATUS OF THE CELLULAR IMMUNITY IN CLEAN-UP WORKERS OF THE CHORNOBYL ACCIDENT AND PATIENTS WITH CANCER OF THE MOUTH CAVITY, ORAL AND LARYNGEAL PART OF THE PHARYNX." Проблеми радіаційної медицини та радіобіології = Problems of Radiation Medicine and Radiobiology 27 (2022): 324–40. http://dx.doi.org/10.33145/2304-08336-2022-27-324-340.
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Objective: to establish cellular immunity in clean-up workers of the Chornobyl accident compared to patients with malignant neoplasms of the oral cavity, oral and laryngeal parts of the pharynx according to the subpopulation organization of peripheral blood leukocytes. Materials and methods. We examined 112 males, age (56,92 ± 6,17) years (M ± SD), including 26 male clean-up workers exposed at the radiation dose 10–500 mSv; 20 male clean-up workers exposed at the dose range 504–990 mSv; 33 patients with malignant neoplasms of the oral cavity, oral and laryngeal parts of the pharynx and 33 nonexposed subjects of the control group. Immune cell subsets analysis was performed by the expression of differential and activation antigens of peripheral blood leukocytes using flow cytometry. Results. In assessing each group's cellular immunity, there was a decrease in the number of CD19+ B-lymphocytes, CD3+HLA-DR+ T- and CD3-HLA-DR+ B-lymphocytes, CD3-16+56+ natural killer cells, combined with an increase in the number of CD8+ T-lymphocytes. An increase in the relative number of CD4+CD8+ T-lymphocytes was determined in clean-up workers (D ≤ 500 mSv) and cancer patients. An increase in the number of CD8+ T-lymphocytes and a decrease in the number of CD4+ T-lymphocytes were observed in clean-up workers (D > 500 mSv), as well as in cancer patients. In addition, a decrease of monocytes, CD3+16+56+, and CD3+TCRαβ+ T-lymphocytes was determined in patients with oncological pathology. Conclusions. The obtained results show the unidirectionality of changes in cellular immunity in the participants of the liquidation of the consequences of the accident at the Chornobyl nuclear power plant and patients with the investigated oncological pathology, which indicates the formation of persistent violations of antitumor protection in the participants of the liquidation of the consequences of the accident as the basis of oncogenesis. Determining changes in the number of lymphocytes, monocytes, granulocytes, CD4+ T-lymphocytes, CD4+CD8+ immature T-lymphocytes, the immunoregulatory ratio CD4+ / CD8+, CD3+TCRαβ+ T-lymphocytes and CD3+16+56+ CTL can have an additional effect on the effectiveness antitumor protection and the probability of oncogenesis risk in immunocompromised individuals. Key words: ionizing radiation, Chornobyl, clean-up workers of the Chornobyl accident, neoplasms of the oral cavity, oral and laryngeal parts of the pharynx, immune system.
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Malickaitė, Radvilė, Laimutė Jurgauskienė, Stanislava Simanavičienė, Vytė Valerija Maneikienė, Rita Sudikienė, and Kęstutis Ručinskas. "Imuninė stebėsena širdies transplantacijoje: atmetimo reakcijos poveikis T limfocitų žymenų ekspresijai." Lietuvos chirurgija 8, no. 3 (2010): 0. http://dx.doi.org/10.15388/lietchirur.2010.3.2103.
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Radvilė Malickaitė, Laimutė Jurgauskienė, Stanislava Simanavičienė, Vytė Valerija Maneikienė, Rita Sudikienė, Kęstutis Ručinskas Vilniaus universiteto Širdies ir kraujagyslių ligų klinikos Širdies chirurgijos centras, Santariškių g. 2, LT-08661 Vilnius El. paštas: Radvile.Malickaite@santa.lt Darbo tikslas: Nustatyti Vilniaus universiteto ligoninės Santariškių klinikų Širdies chirurgijos centre atliekamos širdies transplantacijos įtaką T limfocitų aktyvumo rodikliams, įvertinti imuninės stebėsenos tinkamumą ūminiam transplantato atmetimui prognozuoti. Ligoniai ir metodai: Retrospektyviai analizuotas dvidešimt vieno širdies recipiento imuninių rodiklių kitimas esant normaliai potransplantacinei būklei ir ūminiam transplantato atmetimui. Periferinio kraujo imunokompetentinių ląstelių CD3+CD103+, CD4+CD103+, CD8+CD103+, CD3+CD134+, CD4+CD134+, CD8+CD134+, CD8+CD57+ ir CD8+CD38+ procentas nustatytas tėkmės citometrijos būdu. Ūminis transplantato atmetimas vertintas pagal histologinius endomiokardinės biopsijos radinius. Rezultatai: Esant ūminio atmetimo epizodams, kai endomiokardo biopsijos įvertintos ≥ 2R (3A) laipsniu, reikšmingai didėja integrino CD103 (p < 0,0001), kostimuliacinio receptoriaus CD134 (p = 0,005), antigeno CD57 (p = 0,005) ir ląstelių paviršiaus glikoproteino CD38 (p = 0,015) ekspresija citotoksinių CD8+ limfocitų paviršiuje. Išvados: Imuninė periferinio kraujo limfocitų būklės stebėsena gali būti taikoma po transplantacijos skiriamam imunosupresiniam gydymui įvertinti numatant didelę ūminio atmetimo tikimybę. Reikšminiai žodžiai: kiaušidžių cistos, supiktybėjimo rizika, piktybiškumo rizikos indeksas, ultragarsinis tyrimas, Ca-125 antigenas, chirurginis gydymas Measuring T cell reactivity for predicting heart transplant rejection Radvilė Malickaitė, Laimutė Jurgauskienė, Stanislava Simanavičienė, Vytė Valerija Maneikienė, Rita Sudikienė, Kęstutis Ručinskas Vilnius University, Clinic of Cardiovascular Diseases, Centre of Heart sSurgery, Santariškių str. 2, LT-08661 Vilnius, Lithuania E-mail: Radvile.Malickaite@santa.lt Objective: We aimed to analyze alterations in peripheral blood T-cell subset activation compared with endomyocardial byopsy findings. Patients and methods: The study included in total twenty-one heart recipients grafted 1997–2007 at the Vilnius Heart sSurgery cCenter. T-cell activation markers CD3+CD103+, CD4+CD103+, CD8+CD103+, CD3+CD134+, CD4+CD134+, CD8+CD134+, CD8+CD57+ ir CD8+CD38+ were detected by two-color flow cytometry. Rejection was graded according to the ISHLT (the International Society of Heart and Lung Transplantation) grading system. Results: In case of ≥ 2R (3A) rejection episodes, a significant increase in the expression of integrin CD103 (p < 0.0001), co-stimulatory receptor CD134 (p = 0.005), antigen CD57 (p = 0.005) and surface glycoprotein CD38 (p = 0.015) on CD8+ T lymphocytes has been revealed. Conclusion: Immune monitoring performed on peripheral blood can be used for the assessment of immunosuppression therapy on transplant recipients’ immune response and for determining the risk of rejection. Key words: heart transplantation, acute rejection, and immune activation
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Mahmoud, Fadia, Fatema Habeeb, Nirmina Arifhodzic, David Haines, and Ladislav Novotny. "T Lymphocyte Activation Profiles in Peripheral Blood of Long-Versus Short-Term Residents of Kuwait: Comparison with Asthmatics." Annals of the Academy of Medicine, Singapore 39, no. 11 (2010): 854–60. http://dx.doi.org/10.47102/annals-acadmedsg.v39n11p854.
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Introduction: During the Arabian Gulf Wars of 1991 and 2003, the resident population of Kuwait sustained heavy exposure to environmental toxicants introduced by military activities. No comprehensive studies have been conducted to assess how exposure to the wartime and postwar environment may have altered the fundamental patterns of immune reactivity among Kuwaitis in ways that affect pathogenesis of disease. This present study addresses this issue by characterising immunological features of asthma and allergies in a Kuwaiti population that is unique and possibly correlates with toxicant exposures. Materials and Methods: Twenty-five long-term residents of Kuwait afflicted with bronchial asthma concurrent with rhinitis; and 2 healthy control groups: 18 long-term residents and 10 newcomers to Kuwait were evaluated by 2- and 3-colour flow cytometry for peripheral blood T cell subpopulation frequencies. Results: Relative to healthy, long-term residents, significantly elevated frequencies of all activated cell phenotypes were observed in the blood of the asthmatic group (P <0.05 to P <0.001), except for CD8+HLA-DR+ cells and a presumed T-regulatory (Treg) subpopulation: CD4+CD25high. The asthmatic group was also observed to have larger populations of CD3+ (pan-T cells), CD4+ (T helper cells) and CD8+ (cytotoxic T cells), CD3+CD56 (NKT-like cells) and CD56+CD16+ (NK cells) compared to healthy long-term residents. Compared to healthy recent immigrants, the blood of long-term residents contained elevated levels of CD3+CD56+ (NK-like), CD4+CD45RA+/CD45RO+ (Naive-to-Memory Transitional), but lower CD4+CD25+high (Treg) (P <0.05). Conclusions: Elevated representation of natural killer (NKT)-like and memory phenotypes may predispose long-term residents towards enhanced susceptibility for airway disease; while at the same time, reducing representation of Treg cells which are protective against airway disease, and this may increase vulnerability to these syndromes among the residents of Kuwait. These results may provide insight into the features of immunopathogenesis of asthma and allergies in Kuwait that arise as a result of the special environment of the country. Key words: Asthma, Immune reactivity, Kuwait, Rhinitis, T cells
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Naseeb, Summaira, Parvez Ahmed, Shahzad Nasir, et al. "Immune Reconstitution Following Allogeneic HSCT in Hematological Diseases." Blood 144, Supplement 1 (2024): 7337. https://doi.org/10.1182/blood-2024-201958.
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Objective: To evaluate immune reconstitution following allogeneic hematopoietic stem cell transplantation Study Design: Retrospective cohort study Place and Duration of the Study: Department of Bone Marrow Transplant, Quaid-e-Azam International Hospital Islamabad, Pakistan; from January 2023 to January 2024. Methodology: We evaluated 50 adult and pediatric patients undergoing allogeneic HSCT for malignant and nonmalignant diseases from year 2022 to 2023. Absolute lymphocyte count, lymphocyte subsets and immunoglobulin levels were performed at 6 and 12 months post-transplant. Patients who did not have complete medical records or faced graft failure/rejection before 6 months post-transplant were excluded from the study. Results: In our study absolute lymphocyte count recovery was observed in 94% of patients on D+180 that increased to 100% on D+365. The average number of cells being 2599.84/μL (SD=1281.742) and 2926.96 μL (SD=1065.378) on D+180 and D+365 respectively. By day 180 post-transplant, 84% of patients had achieved CD3+CD8+ recovery, while CD16+CD56+ and CD19+ cell recovery was seen in 74% and 56% cases respectively. Day +180 CD3+CD4+ recovery was seen in only 8% patients that increased to 21% by day +365. Normal lymphocyte recovery values at day 365 post-transplant were: CD3+CD8+ in 84% patients, CD16+CD56+ and CD19+ cells in 78% patients. Early CD3+CD4+ recovery was seen in patients with age &lt;18 years, non-malignant diseases and with no or minimal GVHD but the values were not statistically significant. Age adjusted normal immunoglobulin levels for IgG, IgM and IgA by day +180 were achieved in 92%, 90% and 84% patients while after 1 year 98%, 90% and 96% patients had normal levels of IgG, IgM and IgA respectively. Patients receiving CD34+ dose &gt;5 x 106/kg had significantly early neutrophil engraftment (p=0.025); mean 11.40 days (SD 1.67) versus 13.76 days (SD 1.43) for CD34 dose ≤ 5 x 106/kg. . Conclusion: Majority of the patients achieved normal immunoglobulin levels and lymphocyte subsets by day 180 post HSCT while CD3+CD8+ T cell recovery was seen in 8% and 21% patient by day 180 and 365 post-transplant, respectively. Key Words: Hematopoietic stem cell transplant, lymphocyte subsets, immune reconstitution
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Wang, Jing, Xiaoyuan Zhang, Yue Zhao, Jingjing Zhang, Yibin Zhou, and Li Hou. "Study on the Changes of Immune Cells in the Evolution of Myelodysplastic Syndromes." Blood 142, Supplement 1 (2023): 5381. http://dx.doi.org/10.1182/blood-2023-179797.
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ABSTRACT Objective: To analyze the differences of peripheral blood immune cells in healthy people, and patients with myelodysplastic syndromes (MDS) patients or acute myeloid leukemia (AML), and to investigate their correlations with International Prostate Symptom Score(IPSS), blasts and other factors in MDS patients. Methods: 55 patients with MDS(MDS group), 67 patients with AML(AML group) and 25 heathy people(healthy group) were selected as research objects from Dongzhimen Hospital Affiliated with Beijing University of Chinese Medicine from January, 2012 to April, 2022 for this retrospective analysis. By comparing peripheral blood lymphocyte subsets count and peripheral blood lymphocyte subsets percentage, we studied the expression of T lymphocyte subsets (CD3+, CD4+, CD8+, CD4+/CD8+, CD3+CD4+CD25+FoxP3+), B cells (CD19+) and NK cells (CD3-CD16+CD56+). The correlations between the different indexes and IPSS and blasts were analyzed. Results: (1) Peripheral blood lymphocyte subsets count: There were differences among the three groups. Through pairwise comparison, MDS group had lower peripheral blood lymphocyte subsets count(P＜0.025), probably owing to the low peripheral blood lymphocyte count. (2)Peripheral blood lymphocyte subsets percentage: The percentages of Treg cells, B cells and T cells varied among the three groups. Compared with the healthy group, the percentages of T cells ( P＜0.025) and Treg cells ( P＞0.025) in MDS group were higher, and the percentage of B cells ( P＜0.025) was lower. Compared with MDS group, the percentage of Treg cells ( P＜0.025) in AML group was higher and the proportion of B cells was lower ( P＜0.025). (3)Correlations with IPSS and blasts: The percentage of Treg cells was positively correlated with IPSS and blasts. Conclusion: Treg cells showed an increasing trend in turn by healthy people group, MDS group and AML group. The high expression of Treg cells may have great significance in prognosis and progression of MDS. Key words: Acute myeloid leukemia; Blasts; Immune cells; Myelodysplastic syndromes.
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Zvarych, L. M., V. V. Panchenko, N. A. Golyarnik, O. A. Belayev, and D. A. Bazyka. "THE INFLUENCE OF THE WAR FACTOR ON AGE CHANGES IN THE SUBPOPULATION STRUCTURE OF LEUCOCYTES AS A COMPONENT OF INFLAMMAGING IN THE GROUPS OF MILITARY PERSONNEL AND CLEAN-UP WORKERS OF THE CHORNOBYL ACCIDENT." Проблеми радіаційної медицини та радіобіології = Problems of Radiation Medicine and Radiobiology 28 (2023): 286–315. http://dx.doi.org/10.33145/2304-8336-2023-28-286-315.
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Objective: to evaluate the subpopulation structure of peripheral blood leukocytes as a component of inflammaging in the servicemen of the Armed Forces of Ukraine and clean-up workers of the accident at the Chornobyl nuclear power plant. Materials and methods. Three hundred ninety-seven males aged 30–82 (54.99 ± 8.85) years examined. Among them: clean-up workers of the accident at the Chornobyl nuclear power plant, aged 54–64 (59.95 ± 1.88) years, who were examined before the beginning and during the Russian military invasion of Ukraine; 24 civilians of the control group, aged 50–77 (58.87 ± 7.18) years and 15 persons of the age control group, aged 42–49 (46.00 ± 2.26) years, who examined before the war has started; the comparison group–W – 27 civilians (age: 51–82 (65.44 ± 9.13)) and the age control group–W – 14 civilians (age: 31–49 (40.64 ± 5.59)) who examined after the war has started; 73 servicemen of the Armed Forces of Ukraine aged 30–49 (42.20 ± 5.35) years and 54 servicemen aged 50–59 (54.07 ± 2.86) years. The subpopulation distribution of peripheral blood leukocytes was analyzed using flow cytometry. Results. A decrease in the relative number of CD45+14- granulocytes, CD3+HLA-DR+ T-lymphocytes, and CD3-57+ NK against the background of an increase in the number of CD45+14+ monocytes, CD3-HLA-DR+ B-lymphocytes found in the majority of people in the examination groups, the study of which conducted after the beginning of the war, CD4+ T-lymphocytes and CD3+57+ NKT. The relative number of CD45+14- granulocytes increased against the background of a decrease in CD45+14- lymphocytes was noted in clean-up workers who were examined before the war started. About 30% of civilians over 50 years of age who were examined before the war started had a decrease in CD19+ B- lymphocytes, CD4+ and CD8+ T-lymphocytes, CD3-57+ NK, CD3+57+ NKT, and the CD4+ / CD8+ ratio. Conducted correlation analysis revealed a weak to moderate inverse correlation between the relative number of CD19+ B-lymphocytes and age in civilians, regardless of when they were examined, and the servicemen of the Armed Forces of Ukraine. Inverse correlations of HLA-DR+ B-lymphocytes, CD45+14+ monocytes, CD4+ T-lymphocytes, CD4+ / CD8+ ratio, and CD3-57+ NK and CD3+57+ NKT with age were noted in civilians who were examined after the war has started. A direct correlation between CD3+57+ NKT and age was found in the servicemen of the Armed Forces of Ukraine. Conclusions. The immunological profile of both clean-up workers in the Chornobyl nuclear power plant and the servicemen of the Armed Forces of Ukraine or civilians was similar compared to the group with reference indicators. An intergroup study revealed changes in the subpopulation distribution of peripheral blood leukocytes, which may be associated with both aging and changes in living conditions during military operations. Specific changes in cellular immunity can either tip the balance towards inflammaging or indicate already existing immunopathological reactions. Key words: ionizing radiation, Chornobyl, clean-up workers of the accident at the Chornobyl nuclear power plant, military servicemen, war, immune system, inflammaging, stress.
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Lolopayung, Selin, Asnani Asnani, and Kobajashi Togo Isamu. "STUDI FORMULASI RUMPUT LAUT (Kappaphycus alvarezii) DAN TEPUNG SAGU (Metroxylon sp.) TERHADAP KOMPOSISI KIMIA, STABILITAS DAN SIFAT SENSORI PADA PRODUK CENDOL RUMPUT LAUT." Jurnal Fish Protech 2, no. 1 (2019): 1. http://dx.doi.org/10.33772/jfp.v2i1.6444.
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AbstrakPenelitian ini bertujuan untuk mengetahui pengaruh perbandingan bubur rumput laut dan tepung sagu terhadap kandungan kimia, sensori dan stabilitas cendol rumput laut, metode penelitan ini menggunakan rancangan acak lengkap (RAL) dan uji Kruskal Wallis yang terdiri dari 3 perlakuan yaitu dengan variasi penambahan konsentrasi rumput laut (R) dan tepung sagu (S) yang berbeda dimana perlakuan RS1 (R=30% : S=70%), RS2 (R=20% : S=80%) dan RS3 (R=10% : S=90%). Kandungan air, abu dan serat diuji menggunakan metode Gravimetri, kandungan karbohidrat Spektrofotometri, uji stabilitas menggunakan metode Saade dan Aslamyah dan uji sensori menggunakan metode Kruskal Wallis (SPSS 16.0). Hasil uji kandungan air, abu dan serat tertinggi terdapat pada perlakuan RS1 dengan nilai 85,50; 0,12 dan 1,76%. Kandungan karbohidrat tertinggi terdapat pada perlakuan RS3 dengan nilai 16,08% dan hasil nilai stabilitas tertinggi terdapat pada perlakuan RS3 dengan waktu 275 menit. Hasil penelitian ini menunjukkan bahwa tidak terdapat pengaruh pada kandungan kimia air, abu, serat dan uji stabilitas, sedangkan kandungan karbohidrat terdapat penggaruh nyata yang ada pada cendol rumput laut pada setiap perlakuan. Kata kunci : Cendol, rumput laut, tepung sagu, komposisi kimia, uji sensori AbstractThis study aims to determine the effect of the comparison of seaweed and sagu flour on the chemical, sensory and stability content of seaweed cendol, this research method uses a completely randomized design (CDR) and non-parametric (Kruskal Wallis) consisting of 3 treatments with additional variations Different concentrations of seaweed (R) and sagu flour (S) where RS1 (R=30%: S=70%), RS2 (R=20%: S=80%) and RS3 (R=10%: S=90%). Water, ash and fiber content were tested by the Gravimetric method, carbohydrate content was tested by the spectrophotometric, stability test tested by Saade and Aslamyah methods and sensory tests by the Kruskal Wallis method (SPSS 16.0). The higher test results of water, ash and fiber content were found in the treatment of RS1 with a value of 85.50; 0.12 and 1.76%, the higher carbohydrate content was found in the RS3 treatment with a value of 16.08% and the higher stability test results were found on RS3 treatment with 275 minutes. The study indicate that there is no effect on the chemical content of water, ash, fiber and stability test, and the carbohydrate content of the significant effect is present in seaweed cendol in each treatment. Key words: Cendol, seaweed, sagu flour, chemical composition, sensory test
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Lolopayung, Selin, Asnani Asnani, and Kobajashi Togo Isamu. "STUDI FORMULASI RUMPUT LAUT (Kappaphycus alvarezii) DAN TEPUNG SAGU (Metroxylon sp.) TERHADAP KOMPOSISI KIMIA, STABILITAS DAN SIFAT SENSORI PADA PRODUK CENDOL RUMPUT LAUT." Jurnal Fish Protech 2, no. 1 (2019): 1. http://dx.doi.org/10.33772/jfp.v2i1.6444.
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AbstrakPenelitian ini bertujuan untuk mengetahui pengaruh perbandingan bubur rumput laut dan tepung sagu terhadap kandungan kimia, sensori dan stabilitas cendol rumput laut, metode penelitan ini menggunakan rancangan acak lengkap (RAL) dan uji Kruskal Wallis yang terdiri dari 3 perlakuan yaitu dengan variasi penambahan konsentrasi rumput laut (R) dan tepung sagu (S) yang berbeda dimana perlakuan RS1 (R=30% : S=70%), RS2 (R=20% : S=80%) dan RS3 (R=10% : S=90%). Kandungan air, abu dan serat diuji menggunakan metode Gravimetri, kandungan karbohidrat Spektrofotometri, uji stabilitas menggunakan metode Saade dan Aslamyah dan uji sensori menggunakan metode Kruskal Wallis (SPSS 16.0). Hasil uji kandungan air, abu dan serat tertinggi terdapat pada perlakuan RS1 dengan nilai 85,50; 0,12 dan 1,76%. Kandungan karbohidrat tertinggi terdapat pada perlakuan RS3 dengan nilai 16,08% dan hasil nilai stabilitas tertinggi terdapat pada perlakuan RS3 dengan waktu 275 menit. Hasil penelitian ini menunjukkan bahwa tidak terdapat pengaruh pada kandungan kimia air, abu, serat dan uji stabilitas, sedangkan kandungan karbohidrat terdapat penggaruh nyata yang ada pada cendol rumput laut pada setiap perlakuan. Kata kunci : Cendol, rumput laut, tepung sagu, komposisi kimia, uji sensori AbstractThis study aims to determine the effect of the comparison of seaweed and sagu flour on the chemical, sensory and stability content of seaweed cendol, this research method uses a completely randomized design (CDR) and non-parametric (Kruskal Wallis) consisting of 3 treatments with additional variations Different concentrations of seaweed (R) and sagu flour (S) where RS1 (R=30%: S=70%), RS2 (R=20%: S=80%) and RS3 (R=10%: S=90%). Water, ash and fiber content were tested by the Gravimetric method, carbohydrate content was tested by the spectrophotometric, stability test tested by Saade and Aslamyah methods and sensory tests by the Kruskal Wallis method (SPSS 16.0). The higher test results of water, ash and fiber content were found in the treatment of RS1 with a value of 85.50; 0.12 and 1.76%, the higher carbohydrate content was found in the RS3 treatment with a value of 16.08% and the higher stability test results were found on RS3 treatment with 275 minutes. The study indicate that there is no effect on the chemical content of water, ash, fiber and stability test, and the carbohydrate content of the significant effect is present in seaweed cendol in each treatment. Key words: Cendol, seaweed, sagu flour, chemical composition, sensory test
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Naw, Naw, and Ei Ei Hlaing. "Relevant Words Extraction Method for Recommendation System." Bulletin of Electrical Engineering and Informatics 2, no. 3 (2013): 169–76. http://dx.doi.org/10.11591/eei.v2i3.200.
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Nowadays, E-commerce is very popular because of information explosion. Text mining is also important for information extraction. Users are more preferable to use the convenience system from many sources such as through web pages, email, social network and so on. This system proposed the relevant words extraction method for car recommendation system from user email. In relevant words extraction, this system proposed the Rule-based approach in Compiling Technique. Context- free grammar is the most suitable for relevant words extraction. Recommendation System (RS) is a most popular tool that helps users to recommend according to their interests. This system implements efficient recommendation system by using proposed key extraction algorithm, Content-based Filtering (CBF) method and Jaccard Coefficient that will help the users who want to buy the car by providing relevant car information.
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Malickaitė, Radvilė, Aldona Stanevičienė, Kęstutis Ručinskas, Laimutė Jurgauskienė, Saulius Miniauskas, and Vytautas Sirvydis. "Imuninė sensitizacija po dirbtinės širdies prijungimo." Lietuvos chirurgija 5, no. 3 (2007): 0. http://dx.doi.org/10.15388/lietchirur.2007.3.2208.
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Radvilė Malickaitė1,2, Aldona Stanevičienė2, Kęstutis Ručinskas1,2, Laimutė Jurgauskienė1,2, Saulius Miniauskas1,2, Vytautas Sirvydis1,21 Vilniaus universiteto Širdies ir kraujagyslių ligų klinikos Širdies chirurgijos centras,Santariškių g. 2, LT-08661 Vilnius2 Vilniaus universiteto ligoninės Santariškių klinikos, Santariškių g. 2, LT-08661 VilniusEl paštas: radvile.malickaite@santa.lt Tikslas Įvertinti Vilniaus universiteto ligoninės Santariškių klinikų Širdies chirurgijos centre atliekamos dirbtinės širdies implantavimo procedūros įtaką imuninei širdies laukiančių recipientų būklei ir palyginti gautus rezultatus su kitų centrų duomenimis. Ligoniai ir metodai Ištirta aštuonių potencialių širdies recipientų, kurių kraujotaka buvo palaikoma dirbtinės širdies skilvelio (DŠS), imuninių rodiklių dinamika: prieš DŠS implantavimą ir praėjus ne mažiau kaip vienam mėnesiui po operacijos. Nustatytas periferinio kraujo imunokompetentinių ląstelių CD3+CD4+ ir CD3+CD8+ skaičius, Apo-1/FAS (CD95) ekspresija CD4+ ir CD8+ limfocitų paviršiuje, viduląstelinė aktyvi kaspazė-3 ir sensitizacija HLA antigenams. Rezultatai Po DŠS prijungimo atsiranda limfocitų T helperių (CD3+CD4+) skaičiaus trūkumas, ryškesnis pacientų, kuriems implantuotas išorinis dirbtinis skilvelis. Numanomas ląstelių praradimo mechanizmas yra apoptozės indukcija. Nepaisant ilgalaikio DŠS prijungimo ir pakartotinių kraujo produktų perpylimų (lentelė), antikūnai prieš HLA sistemos antigenus daugumai pacientų nesigamino: sensitizacija nustatyta tik dviem iš aštuonių pacientų (gavusių santykiškai daugiau kraujo produktų). Išvada Sensitizacija apsunkina pacientų gydymą, ypač vengtini kraujo produktai. Patartina naudoti leukofiltruotus kraujo produktus. Pagrindiniai žodžiai: dirbtiniai širdies skilveliai, imunologinė sensitizacija, apoptozė Comparison of quality of life and physical condition of heart failure Radvilė Malickaitė1,2, Aldona Stanevičienė2, Kęstutis Ručinskas1,2, Laimutė Jurgauskienė1,2, Saulius Miniauskas1,2, Vytautas Sirvydis1,21 Vilnius University Clinic of Cardiovascular Diseases, Cardiac Surgery Centre,Santariškių str. 2, LT-08661 Vilnius, Lithuania2 Vilnius University Hospital „Santariškių klinikos“, Santariškių str. 2, LT-08661 Vilnius, LithuaniaE-mail: radvile.malickaite@santa.lt Objective This study was designed to characterize alterations in the immune system of patients who received artificial heart (AH) placement therapy for end-stage heart failure as a bridge to heart transplantation at the Vilnius University Cardial Surgery Centre. Patients and methods Immunological studies were performed in 8 pts awaiting cardiac transplantation with AH assist (7 of them were transplanted after 75–1050 days of support). Immunological indices before AH placement and after one month of mechanical assistance (period free from infection) were compared. Fluorochrome-labeled Mabs were used for immunophenotypic analysis of circulating T cells and apoptocic activity evaluation. Antibodies against HLA class I molecules were detected by a standard microlymphocytotoxic test. Results AH recipients showed relative lymphopenia, reduction in CD4 T cell counts, a decreased CD+4+ / CD8+ ratio. Six of the eight recipients with long-term support did not produce HLA antibodies despite receiving blood products during support. Conclusions In conclusion, these results suggest that reduction in CD4+ T cell levels accompanying AH support may increase the prevalence of infectious complications and HLA sensitization. Key words: heart-assist devices, immune, sensitization, apoptosis
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Nesterova, I. V., V. N. Gorodin, G. A. Chudilova, et al. "Defects in the interferon system associated with a deficiency of natural killers and T-cells in patients with COVID-19." Infekcionnye bolezni 20, no. 2 (2022): 23–32. http://dx.doi.org/10.20953/1729-9225-2022-2-23-32.
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Identification of interrelated innate and sensitive immune responses in COVID-19 is an important step in the development of targeted therapeutic approaches, which is relevant. Objective. To clarify the features of the defect in the function of NK cells, T lymphocytes, the interferon system in patients with moderate and severe COVID-19. Patients and methods. Tests of the peripheral blood of 50 COVID-19 patients aged 61(57–71) and having the moderate and severe disease were performed. The following parameters were measured: the quantity of CD3+CD19–, CD3+CD4+, CD3+CD8+ T lymphocytes, NK – (CD3–CD16+CD56+), and TNK – CD3+CD16+CD56+ with expression density considered membrane receptors (MFI) (FC 500 Beckman Coulter, USA), the levels of IFN-α, IFN-γ, IL-6, TNF-α cytokines (IFA). Results. Combined immunodeficiency associated with quantitative and functional defects in NK, T lymphocytes and their subsets was revealed in moderate and severe COVID-19. An imbalance of cytokines has been established: blockade of the production of IFN-α and IFN-γ against the background of a significant increase in IL-6 and TNF-α, which negatively affects both the number and functionality of the participants in the immune response and is associated with a severe course and poor prognosis of COVID-19. Conclusion. The data obtained demonstrate the need to develop new strategies and tactics for the treatment of COVID-19, including replacement systemic therapy with recombinant IFN-α2b in combination with antioxidants (Viferon®) in adequate therapeutic doses, aimed at restoring the normal functioning of T lymphocytes, NK and the interferon system. Key words: COVID-19, interferons, moderate and severe disease course, NK cells, T lymphocytes
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Hucl, Pierre, and Maria Matus-Cádiz. "CDC EMDR-4, CDC EMDR-9, and CDC EMDR-14 spring wheats." Canadian Journal of Plant Science 82, no. 2 (2002): 411–13. http://dx.doi.org/10.4141/p01-114.
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Early-maturing spring wheat germplasm lines CDC EMDR-4, CDC EMDR-9, and CDC EMDR-14 have high levels of seed dormancy. Their agronomic performance is comparable to that of the cultivar Columbus. These three lines had similar grain protein concentration and kernel hardness relative to the check cultivars, except CDC EMDR-4, which had a soft endosperm texture. Key words: Triticum aestivum L., germplasm, seed dormancy, pre-harvest sprouting resistance
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Rico- Lombana, Alejandra Rico Lombana, Jose Mateus, Paola Lasso, John Mario González, Concepción Judith Puerta, and Adriana Cuéllar. "Expression of inhibitory receptors on CD4+ and CD8+ T cells from healthy Colombian donors." Universitas Scientiarum 17, no. 1 (2012): 35. http://dx.doi.org/10.11144/javeriana.sc17-1.eoir.
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<p>T cell activation involves positive cellular signals that promote effector functions and negative signals that contribute to the regulation of these responses. These regulatory signals are generated upon activation of receptors on T cells that include CD160, 2B4, Programmed Death-1 and CTLA-4. <strong>Objective</strong>. To evaluate the expression of inhibitory receptors like CD160, 2B4, Programmed Death-1 and CTLA-4 on CD4+ and CD8+ T cells from healthy Colombian donors. <strong>Materials and methods</strong>. Peripheral blood mononuclear cells from 30 healthy donors from Bogotá (Colombia) were obtained via Ficoll-Hypaque density gradient and cells were stained with specific conjugated antibodies previously titrated. <strong>Results</strong>. The CD160, 2B4, and Programmed Death-1 inhibitory markers were detected on CD4+ T cells<br />with expression levels of 0.35%, 1.04%, and 1.35%, respectively. On CD8+ T cells, these markers were expressed at higher levels: 16%, 8.97%, and 4.3%, respectively. In contrast to the other receptors, CTLA-4 frequency of expression showed no significant difference between CD4+ (1.56%) and CD8+ (1.53%) T cells. Frequency of CD160/2B4 and CTLA-4/ Programmed Death-1 coexpression was 0.18% and 0.09% on CD4+ cells, and 4.02% and 0.2% on CD8+ T cells. <strong>Conclusions</strong>. This is the first report showing the frequency of inhibitory receptors such as CD160, 2B4, Programmed Death-1, and CTLA-4 on CD4+ and CD8+ T cells from healthy Colombian donors. Our findings serve as a baseline for the analysis and comparison of these receptors in Colombian populations with different disease conditions.</p><p><br /><strong>Key words</strong>: inhibitory receptors, T cells</p>
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Balieiro Capela, Ilva Lana, Luciana Santiago de Oliveira, Andressa Karoline Pinto de Lima Ribeiro, Antonio Carlos Rosário Vallinoto, and Izaura Maria Vieira Cayres Vallinoto. "The effects of an exercise program on the response of CD4+ and CD8+ T lymphocytes in people living with HIV/AIDS (PLWHA): a systematic review." Revista de Patologia Tropical / Journal of Tropical Pathology 52, no. 3 (2023): 159–76. http://dx.doi.org/10.5216/rpt.v52i3.75637.
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People living with HIV and AIDS (PLWHA) have decreased functional capacity, muscle strength and quality of life. Furthermore, HIV/AIDS can cause some adverse effects and long-term problems, such as sarcopenia and some peripheral neuropathies. A common practice among the healthy population that helps to modify these risk factors is to practice of some kind of exercise, such as aerobic and resistance exercises. The objective of this review is to assess the possible effects of exercise on the increase in CD4+ and/or CD8+ T lymphocytes among PLWHA. The LILACS, IBECS, PubMed/MEDLINE, Cochrane Library, SciELO, PEDro, ScienceDirect and Bireme databases were systematically searched for randomized controlled trials. The following descriptors were used in the search in English, Portuguese and Spanish: HIV, physical functional performance, resistance training, and aerobic exercise. The methodological quality of the studies was evaluated using the PEDro scale. The data were read, analyzed, extracted, and synthesized. Of the 3,319 studies consulted, only five met the inclusion criteria. A synthesis of the five selected studies was performed, all of which were randomized controlled trials. It was concluded that aerobic and/or resistance exercise protocols have a positive effect on immunity (CD4+ and/or CD8+ T lymphocytes and TNF-α) and on the metabolic/ biochemical profile of PLWHA. KEY WORDS: HIV; AIDS; CD4; CD8; exercise.
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Fowler, D. B. "CDC Kestrel winter wheat." Canadian Journal of Plant Science 77, no. 4 (1997): 673–75. http://dx.doi.org/10.4141/p96-193.
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CDC Kestrel is a lodging-resistant, high-yielding, semidwarf winter wheat with good winterhardiness and rust tolerance that is superior to Norstar. CDC Kestrel is eligible for grades of the Canada Western Red Winter Wheat class. Key words: Triticum aestivum L., cultivar description, wheat (winter)
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Saparov, Karimjon Dusanovich. "CAR MAINTENANCE AND SERVICE." International journal of advanced research in education, technology and management 2, no. 2 (2023): 397–400. https://doi.org/10.5281/zenodo.7654848.
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Aminu, A. I., and N. L. Habib. "Studies on some immunological profile of patients infected with tuberculosis attending infectious diseases hospital, Kano." Bayero Journal of Pure and Applied Sciences 12, no. 1 (2020): 5–12. http://dx.doi.org/10.4314/bajopas.v12i1.2s.
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The magnitude of the immune response to tuberculosis (TB) infection also involves cell-mediated immunity comprising numerous types of T-cell subsets and this reflects the magnitude of the bacterial load and those with the highest load are at a greater risk of developing TB disease. The study assesses the immunological profile (CD4+, CD8+, CD3+, CD45+) of TB infected individuals with drug sensitive TB (DS-TB) and compares it with that of subjects with Latent Tuberculosis Infection (LTBI) and those with Rifampicin Resistant TB (RR-TB). The study was a cross-sectional study conducted at Infectious Diseases Hospital (IDH), Kano. Two hundred and five (205) subjects participated in the study. Collected sputum samples were subjected to Ziehl-Neelsen (ZN) staining and cultured on Lowenstein Jensen medium for the identification of Mycobacteria tuberculosis. Molecular detection of M. tuberculosis and RR-TB was performed using Xpert® MTB/RIF. Blood samples were also collected and the T-cell counts were determined using flow cytometry. The result of the study indicated that 131 of the studied subjects were males and 74 were females and that majority of them were in the group 14-24years (41.0%), 25-34 years (35.6%) and 35-44 years (13.7%) respectively. Among the DS-TB and RR-TB groups majority were males (70.8% and 66.7% respectively). It was shown that 86.2% and 88.9% of the samples from the DS-TB and RR-TB were AFB positive. However, all the 65 samples of DS-TB and 54 samples of the RR-TB were identified as MTB by culture. All the DS-TB samples were detected as susceptible TB by Xpert® MTB/RIF, and all the samples of the RR-TB were confirmed as rifampicin resistant by the Xpert® MTB/RIF. The study also revealed that the T-cell (CD4+, CD8+, CD3+ and CD45+) counts of the studied subjects varied significantly among the different groups of the study (p<0.05) and that compared to the control and the LTBI groups the DS-TB and the RR-TB groups recorded the lowest counts in the order; Control>LTBI>DS-TB>RR-TB.
 Key words: Tuberculosis, T-cells counts, Drug susceptible TB, Latent TB, RR-TB.
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Vandenberg, A., C. Vera, L. Buchwaldt, et al. "CDC LeMay lentil." Canadian Journal of Plant Science 85, no. 1 (2005): 159–60. http://dx.doi.org/10.4141/p03-046.
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CDC LeMay is a high-yielding, yellow cotyledon lentil (Lens culinaris Medik.) cultivar developed by the Crop Development Centre, University of Saskatchewan, Saskatoon, Saskatchewan. It is suitable for the French Green market class of lentil and is intended for cultivation in all lentil-growing areas of western Canada. CDC LeMay was issued registration #5552 on October 28, 2002 by the Variety Section, Plant Health and Plant Products Division, Canadian Food Inspection Agency. Key words: Lentil, cultivar description, Lens culinaris
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Vandenberg, A., C. Vera, L. Buchwaldt, et al. "CDC Plato lentil." Canadian Journal of Plant Science 85, no. 1 (2005): 161–62. http://dx.doi.org/10.4141/p03-047.
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CDC Plato is a high-yielding, yellow cotyledon lentil (Lens culinaris Medik.) cultivar developed by the Crop Development Centre, University of Saskatchewan, Saskatoon, Saskatchewan. It is suitable for the large green market class of lentil and is intended for cultivation in all lentil-growing areas of western Canada. CDC Plato was issued registration #5554 on November 1, 2002 by the Variety Section, Plant Health and Plant Products Division, Canadian Food Inspection Agency. Key words: Lentil, cultivar description, Lens culinaris
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Vandenberg, A., F. A. Kiehn, C. Vera, et al. "CDC Robin lentil." Canadian Journal of Plant Science 82, no. 1 (2002): 111–12. http://dx.doi.org/10.4141/p01-003.
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CDC Robin is a high-yielding, red cotyledon lentil (Lens culinaris Medik.) cultivar developed by the Crop development Centre, University of Saskatchewan, Saskatoon, Saskatchewan. It has brown seed coat colour suitable for the small red market class of lentil. It is intended for cultivation in all lentil-growing areas of western Canada. CDC Robin was issued registration #4990 on 4 October 1999 by the Variety Section, Plant Health and Plant Products Division, Canadian Food Inspection Agency. Key words: Lentil, cultivar description, Lens culinaris
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Vandenberg, A., C. Vera, L. Buchwaldt, et al. "CDC Sedley lentil." Canadian Journal of Plant Science 85, no. 1 (2005): 163–64. http://dx.doi.org/10.4141/p03-048.
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CDC Sedley is a high-yielding, early-maturing, yellow cotyledon lentil (Lens culinaris Medik.) cultivar developed by the Crop Development Centre (CDC), University of Saskatchewan, Saskatoon, Saskatchewan. It is suitable for the large green market class of lentil and is intended for cultivation in all lentil-growing areas of western Canada. CDC Sedley was issued registration #5319 on 2001 May 01 by the Variety Section, Plant Health and Plant Products Division , Canadian Food Inspection Agency. Key words: Lentil, cultivar description, Lens culinaris
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Vandenberg, A., S. Banniza, T. D. Warkentin, et al. "CDC Redberry lentil." Canadian Journal of Plant Science 86, no. 2 (2006): 497–98. http://dx.doi.org/10.4141/p05-071.
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CDC Redberry is a high-yielding, red cotyledon lentil (Lens culinaris Medik.) cultivar developed by the Crop Development Centre, University of Saskatchewan, Saskatoon, Saskatchewan. It has gray seed coat colour, suitable for the red market class of lentil. It is intended for cultivation in all lentil growing areas of western Canada. CDC Redberry was issued registration No.5771 on 2003 Dec. 19 by the Variety Section, Plant Products Division, Canadian Food Inspection Agency. Key words: Lentil, cultivar description, Lens culinaris
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S, Kordeva, and Tchernev G. "Mycosis fungoides type d’emblee after henna dye - case report and an update." Journal of Clinical Research in Dermatology 11, no. 2 (2024): 1–7. http://dx.doi.org/10.15226/2378-1726/11/2/001158.
Full textAbstract:
Mycosis fungoides is a cutaneous T-cell lymphoma, arising from peripheral epidermotropic T-cells that express a CD4+ immunophenotype, with a high CD4/CD8 ratio. Initially manifesting as patches, the condition progresses into infiltrated plaques and can eventually lead to tumor formations. Double positive immunophenotypes, although rare, could be considered as potential predictors for better survival rates. Environmental exposure to radiation and chemicals, along with other risk factors, may be potentially linked to the development of Mycosis fungoides. We present a 74-year-old female with potentially henna-induced Multicentric Mycosis Fungoides type tumor d’emblee, staged T3N0M0B1 lA. Immunohistochemistry and flow cytometry revealed a double positive immunophenotype CD4+/CD8+. Throughout her routine follow-ups, two biopsies were conducted to confirm the diagnosis, with several changes of the therapeutic regimen, all resulting in disease progression. On her latest follow-up, we recommended initiating therapy with methotrexate. We will briefly review the clinical presentation and treatment approaches for a rare and treatment-resistant case of MF, with a primary focus on two newly emerged hypotheses: 1) the significance of double immunophenotypes as prognostic indicators, and 2) the potential chemical contamination of henna with different carcinogens / mutagens and its subsequent role in MF development and progression. Key words: multicentric mycosis fungoides; tumor d’emblee; double positive immunophenotype; henna dye; tattoos; methotrexate
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Pozniak, C. J., S. L. Fox, and D. R. Knott. "CDC Verona durum wheat." Canadian Journal of Plant Science 89, no. 2 (2009): 321–24. http://dx.doi.org/10.4141/cjps08117.
Full textAbstract:
CDC Verona durum wheat is adapted to the durum production area of the Canadian prairies. This conventional-height durum wheat combines good yield and high grain pigment and protein concentrations and is low in grain cadmium. CDC Verona is strong strawed and has similar maturity and disease resistance as other registered durum cultivars. Key words: Triticum turgidum L. var durum, durum wheat, yield, yellow pigment, cadmium, cultivar description
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Hucl, P., M. Matus-Cadiz, A. Vandenberg, et al. "CDC Maria annual canarygrass." Canadian Journal of Plant Science 81, no. 1 (2001): 115–16. http://dx.doi.org/10.4141/p00-047.
Full textAbstract:
CDC Mar ia annual canarygrass, developed at the University of Saskatchewan, possesses glabrous hulls that reduce the skin irritation encountered by farmers during the harvesting process. CDC Maria has higher test and kernel weights, but lower grain yields relative to the pubescent cultivar Keet. CDC Maria is adapted to the traditional canaryseed growing regions of Saskatchewan, the brown and black soil zones. Key words: Canaryseed, Phalaris canariensis L., cultivar description, glabrous
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Fowler, D. B. "CDC Raptor winter wheat." Canadian Journal of Plant Science 82, no. 2 (2002): 407–9. http://dx.doi.org/10.4141/p01-110.
Full textAbstract:
CDC Raptor is a high-yielding, lodging-resistant, semidwarf winter wheat (Triticum aestivum L.) with good winter hardiness. It has stem and leaf rust resistance that is similar to CDC Falcon and superior to all other winter wheat cultivars registered for production in western Canada. CDC Raptor is eligible for grades of the Canada Western Red Winter Wheat class. Key words: Triticum aestivum L., cultivar description, wheat (winter).
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McHughen, A., G. G. Rowland, F. A. Holm, R. S. Bhatty, and E. O. Kenaschuk. "CDC Triffid transgenic flax." Canadian Journal of Plant Science 77, no. 4 (1997): 641–43. http://dx.doi.org/10.4141/p96-188.
Full textAbstract:
CDC Triffid is a transgenic sulfonylurea herbicide resistant cultivar with agronomic features similar to NorLin. It is intended to provide a sustainable, broadleaf cropping option to summerfallowing or continuous cropping to cereals in soils previously treated with residual sulfonylurea herbicides. CDC Triffid flax was developed at the Crop Development Centre, University of Saskatchewan. Key words: Flax, linseed, Linum usitatissimum L., cultivar description, transgenic, biotechnology, sulfonylurea, herbicide resistance, herbicide residue, germplasm
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Vandenberg, A., F. A. Kiehn, C. Vera, et al. "CDC Glamis lentil." Canadian Journal of Plant Science 82, no. 1 (2002): 103–4. http://dx.doi.org/10.4141/p01-001.
Full textAbstract:
CDC Glamis is a high-yielding, yellow cotyledon lentil (Lens culinaris Medik.) cultivar developed by the Crop Development Centre, University of Saskatchewan. It is intended for cultivation in all lentil production areas of western Canada. It has large seeds with green seed coat and is suitable for the large green market class of lentil. CDC Glamis wa s issued registration #4835 on 8 December 1998 by the Variety Section, Plant Health and Plant Products Division, Canadian Food Inspection Agency. Key words: Lentil, cultivar description, Lens culinaris
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Vandenberg, A., F. A. Kiehn, C. Vera, et al. "CDC Sovereign lentil." Canadian Journal of Plant Science 82, no. 1 (2002): 107–8. http://dx.doi.org/10.4141/p01-004.
Full textAbstract:
CDC Sovereign is a high-yielding, yellow cotyledon lentil (Lens culinaris Medik.) cultivar developed by the Crop Development Centre, University of Saskatchewan, Saskatoon, Saskatchewan. It is intended for cultivation in all lentil-growing areas of western Canada. It has large, plump seeds with green seed coat, suitable for the large green market class of lentil. CDC Sovereign was issued registration #5019 on 26 November 1999 by the Variety Section, Plant Health and Plant Products Division, Canadian Food Inspection Agency. Key words: Lentil, cultivar description, Lens culinaris
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Vandenberg, Albert, Al Slinkard, and Tom Warkentin. "CDC Montero field pea." Canadian Journal of Plant Science 82, no. 1 (2002): 121–22. http://dx.doi.org/10.4141/p01-076.
Full textAbstract:
CDC Montero is a green cotyledon field pea (Pisum sativum L.) cultivar with semi-leafless leaf type, powdery mildew resistance and medium-sized round seed. It was developed by the Crop Development Centre, University of Saskatchewan, Saskatoon, Saskatchewan. The Variety Section, Plant Health and Plant Products Division, Canadian Food Inspection Agency issued registration #5237 to CDC Montero on 12 January 2001. Key words: Field pea, Pisum sativum L., cultivar description, powdery mildew resistance
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Vandenberg, A., F. A. Kiehn, C. Vera, et al. "CDC Grandora lentil." Canadian Journal of Plant Science 82, no. 1 (2002): 105–6. http://dx.doi.org/10.4141/p01-002.
Full textAbstract:
CDC Grandora is a high-yielding, yellow cotyledon lentil (Lens culinaris Medik.) cultivar developed by the Crop Development Centre, University of Saskatchewan, Saskatoon, Saskatchewan. It has large seeds with green seed coat and is suitable for the large green market class of lentil. CDC Grandora is intended for cultivation in all lentil-growing areas of western Canada. CDC Grandora was issued registration #4993 on October 4, 1999 by the Variety Section, Plant Health and Plant Products Division, Canadian Food Inspection Agency. Key words: Lentil, cultivar description, Lens culinaris
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Vandenberg, A., F. A. Kiehn, C. Vera, et al. "CDC Vantage lentil." Canadian Journal of Plant Science 82, no. 1 (2002): 109–10. http://dx.doi.org/10.4141/p01-005.
Full textAbstract:
CDC Vantage is a high-yielding, yellow cotyledon lentil (Lens culinaris Medik.) cultivar developed by the Crop Development Centre, University of Saskatchewan, Saskatoon, Saskatchewan. It is intended for cultivation in all lentil growing areas of western Canada. The seed coat of CDC Vantage is pale green. It resembles CDC Richlea, the current commercial standard in Canada for the medium green market class of lentil. CDC Vantage was issued registration #4834 on 9 December 1998 by the Variety Section, Plant Health and Plant Products Division, Canadian Food Inspection Agency. Key words: Lentil, cultivar description, Lens culinaris
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Rowland, G. G., Y. A. Hormis, and K. Y. Rashid. "CDC Valour flax." Canadian Journal of Plant Science 82, no. 2 (2002): 427–28. http://dx.doi.org/10.4141/p01-064.
Full textAbstract:
CDC Valour, an early-maturing oilseed flax (Linum usitatissimum L.), was developed in 1996 at the Crop Development Centre, University of Saskatchewan, Saskatoon, Saskatchewan. This cultivar has medium oil content, good oil quality, brown seeds and medium seed size, fair lodging resistance and high yield when seeded early in the Black and the Brown Soil zones of the prairies. It is immune to North American races of rust caused by Melampsora lini and moderately resistant to wilt caused by Fusarium oxysporum f. sp. lini. Key words: Flax, oilseed, Linum usitatissimum L., cultivar description
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Rowland, G. G., Y. A. Hormis, and K. Y. Rashid. "CDC Arras flax." Canadian Journal of Plant Science 82, no. 1 (2002): 99–100. http://dx.doi.org/10.4141/p01-065.
Full textAbstract:
CDC Arras is a medium-early-maturing oilseed flax (Linum usitatissimum L.) developed at the Crop Development Centre, University of Saskatchewan, Saskatoon, Saskatchewan. This cultivar has medium oil content, good oil quality, large seed size, fair lodging resistance and high yield when seeded early in the Black and the Brown Soil zones of the prairies. It is immune to North American races of rust caused by Melampsora lini and moderately resistant to wilt caused by Fusarium oxysporum f. sp. lini. Key words: Flax, oilseed, Linum usitatissimum L., cultivar description
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Rowland, G. G., Y. A. Hormis, and K. Y. Rashid. "CDC Bethune flax." Canadian Journal of Plant Science 82, no. 1 (2002): 101–2. http://dx.doi.org/10.4141/p01-066.
Full textAbstract:
CDC Bethune, is a medium-late-maturing oilseed flax (Linum usitatissimum L.) developed by Crop Development Centre, University of Saskatchewan, Saskatoon, Saskatchewan. This cultivar has medium oil content, medium oil quality, medium seed size, good lodging resistance and high yield when seeded early in the Black and the Brown Soil zones of the prairies. It is immune to North American races of rust caused by Melampsora lini and moderately resistant to wilt caused by Fusarium oxysporum f. sp. lini. Key words: Flax, oilseed, Linum usitatissimum L., cultivar description
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Selvaraj, R. K., and G. Cherian. "Changes in delayed type hypersensitivity, egg antibody content and immune cell fatty acid composition of layer birds fed conjugated linoleic acid, n-6 or n-3 fatty acids." Canadian Journal of Animal Science 84, no. 2 (2004): 221–28. http://dx.doi.org/10.4141/a03-058.
Full textAbstract:
The effects of polyunsaturated fatty acids on delayed type hypersensitivity (DTH), egg yolk antibody content, immune tissue fatty acid profile and lipid oxidation products of layer birds were investigated. One hundred and twenty layer birds were fed diets containing conjugated linoleic acid (CLA) + animal fat (Diet I), sunflower oil (Diet II), canola + flax oil (Diet III) or fish oil (Diet IV). The total added lipid content of the diet was 3%. Birds fed Diets III and IV had higher content of n-3 fatty acids in lymphocyte and splenocytes. Thiobarbituric reactive substances were higher (P < 0.05) in the breast and thigh muscle of Diet IV fed birds. Serum and yolk anti-BSA antibody contents were higher (P < 0.05) in birds fed Diets III and IV. DTH was decreased (P < 0.05) in birds fed Diets IV and III. The number of lymphocyte CD4+ and CD8+ cells and spleen mononuclear cell CD4+, CD8+ and IgM+ cells did not differ (P > 0.05) between treatment groups. Feeding n-3 fatty acids increased antibody-mediated immune response, while n-6 fatty acids and CLA increased cell-mediated immune response. Key words: Conjugated linoleic acid, polyunsaturated fatty acids, delayed type hypersensitivity, immunoglobulins
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Lu, Ning, Yui-Hsi Wang, and Yong-Jun Liu. "TSLP and IL-7 use two different mechanisms in regulating human CD4+ T cell homeostasis (82.1)." Journal of Immunology 182, no. 1_Supplement (2009): 82.1. http://dx.doi.org/10.4049/jimmunol.182.supp.82.1.
Full textAbstract:
Abstract Homeostatic proliferation of CD4+ T cells depends on both self-peptide-MHC ligands and cytokines such as interleukin 7 (IL-7) and Thymic Stromal Lymphopoietin (TSLP). Although it is well established that IL-7 directly acts on CD4+ T cells, data on whether TSLP directly induce human CD4+ T cell proliferation and TH2 differentiation are conflicting. We report here that resting and activated CD4+ T cells express high levels of IL-7 receptors, but very low levels of TSLP receptor when compared with that expressed by myeloid dendritic cells (mDC). While IL-7 induce strong STAT1, 3 and 5 activation and promote the proliferation of naïve CD4+ T cells in the presence of anti-CD3 and anti-CD28, TSLP only induce a weak STAT5 activation which is associated with the marginally improved cell survival, but fail to induce CD4+ T cell proliferation and TH2 differentiation. The effect of TSLP on enhancing human T cell proliferation could be observed only when sorted naïve CD4+ T cells were cultured with contaminated mDCs at as low as 0.5% in culture. TSLP could only induce purified naïve CD4+ T cells to differentiate into TH2 cells in the presence allogeneic mDCs. This study demonstrates that IL-7 and TSLP use different mechanisms in regulating human CD4+ T cell homeostasis and underscores the importance of cell purity in immunology research. Key words: interleukin 7, Thymic Stromal Lymphopoietin, T cell homeostasis, proliferation, survival
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Warkentin, Tom, Albert Vandenberg, Sabine Banniza, and Al Slinkard. "CDC Golden field pea." Canadian Journal of Plant Science 84, no. 1 (2004): 237–38. http://dx.doi.org/10.4141/p03-116.
Full textAbstract:
CDC Golden, a yellow cotyledon field pea (Pisum sativum L.) cultivar, was released in 2003 by the Crop Development Centre, University of Saskatchewan for distribution to Select seed growers in Saskatchewan and Alberta through the Variety Release Committee of the Saskatchewan Pulse Growers. CDC Golden has a semileafless leaf type, good lodging resistance, medium-sized, round seeds, and good yielding ability. CDC Golden is adapted to the field-pea-growing region of western Canada. Key words: Field pea, Pisum sativum L., cultivar description
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Albert Vandenberg, Tom Warkentin,, Sabine Banniza, and Al Slinkard. "CDC Striker field pea." Canadian Journal of Plant Science 84, no. 1 (2004): 239–40. http://dx.doi.org/10.4141/p03-117.
Full textAbstract:
CDC Striker, a green cotyledon field pea (Pisum sativum L.) cultivar, was released in 2002 by the Crop Development Centre, University of Saskatchewan, for distribution to Select seed growers in Saskatchewan and Alberta through the Variety Release Committee of the Saskatchewan Pulse Growers. CDC Striker has a semileafless leaf type, good lodging resistance, medium-sized, round seeds, and good yield potential. CDC Striker is adapted to the field pea growing region of western Canada. Key words: Field pea, Pisum sativum L., cultivar description
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Fowler, D. B. "CDC Clair winter wheat." Canadian Journal of Plant Science 77, no. 4 (1997): 669–71. http://dx.doi.org/10.4141/p96-192.
Full textAbstract:
CDC Clair is a high-yielding, strong-strawed, semidwarf winter wheat (Triticum aestivum L.) with good winterhardiness. When grown in western Canada, it has the high grain yield and agronomic performance of CDC Kestrel, but improved grain quality. The grain protein concentration of CDC Clair has been higher than that of CDC Kestrel and similar to Norstar. CDC Clair is eligible for grades of the Canada Western Red Winter Wheat class. Key words: Triticum aestivum L., cultivar description, wheat (winter)
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Mei, Yihan, Yu Liu, Wenbing Liu, et al. "ADGRG1/GPR56 As a Marker of CD8+ Tumor-Reactive T Cells in Acute Myeloid Leukemia." Blood 144, Supplement 1 (2024): 4289. https://doi.org/10.1182/blood-2024-194281.
Full textAbstract:
Key words: AML; tumor-reactive T cell; ADGRG1 Objective: Given the promising clinical efficacy of T cell-based immunotherapy in solid tumors, there is a growing interest in harnessing T cells for treating acute myeloid leukemia (AML). However, the characteristics of tumor-reactive T cells and their unique molecular markers remain unclear. In this study, we aim to explore the specific marker of AML tumor-reactive T cells. Methods: Bone marrow (BM) T cells from newly diagnosed AML patients were collected for scRNA-seq and scTCR-seq. These data were integrated with publicly available healthy donors' single-cell data. Using the STARTRAC-like algorithm, tumor-reactive T cells were identified based on the tumor enrichment index, clonal expansion index, proliferation index, and activation of TCR signaling pathways. Further validation was conducted on experimental approaches like CD33 CAR-T and target cell co-culture models and IFN-γ release assays. RNA-seq data from 42 AML patients were used for further validation by deconvolution. Results: By integrating single-cell data from healthy donors and AML patients, T cells were clustered with specific markers and we focused on CD8+ T cells for further analysis. Using the STARTRAC-like algorithm, these subgroups were categorized into tumor-reactive T cells and bystander T cells. Unlike solid tumors, tumor-reactive T cells in AML were predominantly terminally differentiated effector T cells and did not show significant upregulation of exhaustion markers like PD-1. Instead, they exhibited a senescent-like cytotoxic profile (CD27-CD28-KLRG1+) with upregulation of NK cell-associated molecules. Notably, ADGRG1 was significantly elevated and almost specifically expressed in tumor-reactive T cells compared to bystander T cells. In a conditional knock-in leukemia mouse model, high expression of ADGRG1 was also observed in CD8+ tumor-reactive T cells at the single-cell level. This suggested that ADGRG1 may serve as a unique AML CD8+ tumor-reactive T cell marker. To further validate this hypothesis, anti-CD33 CAR-T and Molm13 were applied as an artificial effector-target cell model. When stimulated by CD3/CD28 Dynabeads or cocultured with K562 (CD33-), neither CAR-T nor vector T cells showed significant ADGRG1 expression. However, cocultured with Molm13 (CD33+), the ADGRG1 was significantly upregulated on anti-CD33 CAR-T cells (p = 0.016), suggesting the TCR-antigen interaction induces the evident ADGRG1 upregulation. Then ADGRG1+/-CD8+ T cells were isolated from the BM samples of newly diagnosed AML patients from various subtypes and co-cultured with the matched patient's BM CD34+ leukemia blast cell. It was observed that ADGRG1+CD8+ T cells secreted significantly higher levels of IFN-γ upon co-culture 48h with blasts, demonstrating the tumor reactivity of ADGRG1+CD8+ T cells. The characteristic of ADGRG1 as a marker for tumor-reactive T cells might be a common feature of tumor-reactive T cells in AML. We also conducted a preliminary exploration into the clinical significance of the ADGRG1+CD8+ T cell subset. By deconvolution based on the single-cell landscape of AML patients, relapsed/refractory patients had lower levels of ADGRG1+CD8+ T cells in their BM (p = 0.004), suggesting that a lower proportion of these cells at the initial diagnosis might indicate a poor prognosis. Conclusions: By drawing on experiences from solid tumor research, we identified tumor-reactive T cells with unique phenotypes in AML and detected ADGRG1 as their marker. Our findings provide a simpler, more feasible approach for future tumor-reactive TCR screening.
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Rowland, G. G., A. G. McHughen, Y. A. Hormis, and K. Y. Rashid. "CDC Normandy flax." Canadian Journal of Plant Science 82, no. 2 (2002): 425–26. http://dx.doi.org/10.4141/p01-063.
Full textAbstract:
CDC Normandy, a tissue-culture-derived oilseed flax (Linum usitatissimum L.) was released in 1995 by the Crop Development Centre, University of Saskatchewan, Saskatoon, Saskatchewan. This cultivar is a medium-early maturing, with medium oil content, good oil quality, brown seeds and medium seed size, fair lodging resistance and high yields when seeded early in the Black and the Brown Soil zones of the prairies. It is immune to North American races of rust caused by Melampsora lini and moderately resistant to wilt caused by Fusarium oxysporum f. sp. lini. Key words: Flax, oilseed, Linum usitatissimum L., cultivar description, somaclonal variation
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Rowland, G. G., Y. A. Hormis, and K. Y. Rashid. "CDC Mons flax." Canadian Journal of Plant Science 83, no. 4 (2003): 801–2. http://dx.doi.org/10.4141/p02-169.
Full textAbstract:
CDC Mons, a mediumlate maturing oilseed flax (Linum usitatissimum L.) was released in 2002 by the Crop Development Centre, University of Saskatchewan, Saskatoon, Saskatchewan. This cultivar has medium oil content, medium oil quality, small seed size, good lodging resistance and high yield when seeded early in the Black Soil zones of the prairies. It is immune to North American races of rust caused by Melampsora lini, moderately resistant to wilt caused by Fusarium oxysporum f. sp. lini and moderately resistant to powdery mildew caused by Oidium lini. Key words: Flax, oilseed, Linum usitatissimum L., cultivar description
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Warkentin, Tom, Albert Vandenberg, Bunyamin Tar’an, Sabine Barlow, and Scott Ife. "CDC Meadow field pea." Canadian Journal of Plant Science 87, no. 4 (2007): 909–10. http://dx.doi.org/10.4141/cjps07038.
Full textAbstract:
CDC Meadow, a yellow cotyledon field pea (Pisum sativum L.) cultivar, was released in 2006 by the Crop Development Centre, University of Saskatchewan for distribution to Select seed growers in Saskatchewan and Alberta through the Variety Release Committee of the Saskatchewan Pulse Growers. CDC Meadow has a semileafless leaf type, good lodging resistance, powdery mildew resistance, medium-sized, round seeds, and good yielding ability. CDC Meadow is adapted to the field pea growing regions of western Canada. Key words: Field pea, Pisum sativum L., cultivar description