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Relevant bibliographies by topics / Celigo image cytometer

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Academic literature on the topic 'Celigo image cytometer'

Author: Grafiati

Published: 4 June 2025

Last updated: 1 August 2025

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Journal articles on the topic "Celigo image cytometer"

1

Chan, Leo, Haohai Zhang, William Rice, et al. "Novel cell-based high-throughput hybridoma screening method using the Celigo image cytometer for antibody discovery." Journal of Immunology 200, no. 1_Supplement (2018): 120.1. http://dx.doi.org/10.4049/jimmunol.200.supp.120.1.

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Abstract Hybridoma screening is a highly important process that can identify potential clones from hybridoma cultures against the desired target antigen. Traditional screening methods using ELISA and flow cytometry presented technical issues such as limited accuracy, reduced high-throughput capability, increased time and cost. Here, we demonstrate a novel cell-based high-throughput screening method using the Celigo Image Cytometer, which avoids nonspecific signals by contrasting antibody binding signals directly on living cells, with and without recombinant antigen expression. The image cytome

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Pierce, Mackenzie, Yongyang Huang, and Bo Lin. "Abstract 485: Using the Celigo™ Image Cytometer for effectively screening rAAV gene delivery methods." Cancer Research 85, no. 8_Supplement_1 (2025): 485. https://doi.org/10.1158/1538-7445.am2025-485.

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Abstract To develop effective rAAV gene delivery vectors, scientists need to consider multiple elements, such as the promoter, insertion location, genetic target, and target gene to name a few. Green fluorescent protein (GFP), or other detection markers are often used to measure vector transduction and gene expression efficiency. Transduced GFP samples can be analyzed using fluorescent microscopy and flow cytometry, however, both methods can be quite time consuming, labor intensive, and lack high-throughput capabilities to kinetically monitor gene expression. In this work, we developed and emp

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Lin, Mong-Shang, Carolina Franco Nitta, Mackenzie Pierce, Bo Lin, Leo L. Chan, and Jessie Ni. "Abstract 3168: High-throughput hybridoma monoclonality characterization using the Celigo™ Image Cytometer." Cancer Research 85, no. 8_Supplement_1 (2025): 3168. https://doi.org/10.1158/1538-7445.am2025-3168.

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Abstract Hybridomas are hybrid cells produced by fusing an antibody producing B cell with a myeloma cell to produce a specific monoclonal antibody. In the process of generating stable clones, developers are often required to perform several rounds of subcloning by plating cultures with a density of a single cell per well. The wells with a single colony are then selected to produce the target antibodies. Traditionally, this lengthy process was heavily relied on researchers with extensive training and experience; however, even experienced researchers may suffer from human error of selecting clon

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Liao, Wen-Chieh, Julia Abdalla, Carolina Franco Nitta, Mackenzie Pierce, Bo Lin, and Jessie Ni. "Abstract 5473: Development of a novel fluorescent ELISpot assay using the CeligoTMImage Cytometer." Cancer Research 85, no. 8_Supplement_1 (2025): 5473. https://doi.org/10.1158/1538-7445.am2025-5473.

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ELISpot and its variant, fluorescent ELISpot (FluoroSpot), are commonly used methodologies that monitor and detect cytokine and/or antibody secretion from peripheral blood mononuclear cells (PBMCs). Conventional ELISpot and FluoroSpot assays utilize PVDF-membrane plates, which require labor-intensive washing steps that may introduce variability in performance and results. Here, we have developed a novel FluoroSpot assay using polystyrene-, clear-bottom microplates with simplified automated washing steps to minimize operator variability and error. In this work, we assessed multiple critical cel

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Cribbes, Scott, Sarah Kessel, Scott McMenemy, Jean Qiu, and Leo Li-Ying Chan. "A Novel Multiparametric Drug-Scoring Method for High-Throughput Screening of 3D Multicellular Tumor Spheroids Using the Celigo Image Cytometer." SLAS DISCOVERY: Advancing the Science of Drug Discovery 22, no. 5 (2017): 547–57. http://dx.doi.org/10.1177/2472555217689884.

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Three-dimensional (3D) tumor models have been increasingly used to investigate and characterize cancer drug compounds. The ability to perform high-throughput screening of 3D multicellular tumor spheroids (MCTS) can highly improve the efficiency and cost-effectiveness of discovering potential cancer drug candidates. Previously, the Celigo Image Cytometer has demonstrated a novel method for high-throughput screening of 3D multicellular tumor spheroids. In this work, we employed the Celigo Image Cytometer to examine the effects of 14 cancer drug compounds on 3D MCTS of the glioblastoma cell line

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Zhang, Haohai, Leo Li-Ying Chan, William Rice, et al. "Novel high-throughput cell-based hybridoma screening methodology using the Celigo Image Cytometer." Journal of Immunological Methods 447 (August 2017): 23–30. http://dx.doi.org/10.1016/j.jim.2017.04.003.

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Chan, Leo, Kai W. Wucherpfennig, and Lucas De Andrade. "Long term time-course monitoring of NK cell-mediated ADCC using the Celigo Image Cytometer." Journal of Immunology 204, no. 1_Supplement (2020): 88.4. http://dx.doi.org/10.4049/jimmunol.204.supp.88.4.

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Abstract Antibody-dependent cell-mediated cytotoxicity (ADCC) assay has been widely performed for immuno-oncological research. Traditionally, ADCC assays are conducted by measuring the amount of released Chromium, calcein AM, or Lactate dehydrogenase (LDH) molecules after the target cancer cells are killed by effector/antibody pair. These methods can be inaccurate due to indirect measurement of supernatant at the end point of 4 hour, however, there is a need to characterize the effects of antibody-dependent cytotoxicity for longer than 72 hours. In this work, we demonstrated the time-course mo

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Chan, Leo, Kelsey McCulley, and Pinaki Banerjee. "A high-throughput image cytometry-based screening method for the detection of IL2-induced peripheral blood mononuclear cell-mediated cytotoxicity." Journal of Immunology 196, no. 1_Supplement (2016): 143.11. http://dx.doi.org/10.4049/jimmunol.196.supp.143.11.

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Abstract Cell-mediated cytotoxicity assays have been an important functional test for investigating the cytotoxic effect of immune effector on target cancer cells. Cytotoxicity assays have traditionally been performed using the 51Chromium (51Cr) release assay, which involves labeling the tumor cells (target) with radioisotopes. The 51Chromium release assay is highly hazardous, time-consuming, and can only acquire end point readout. Furthermore, it can generate inconsistent results due to batch variation, ability of the target cell for 51Cr uptake, and low sensitivity. In this work, we demonstr

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9

Jiang, Wen, W. Nathaniel Brennen, Samuel R. Denmeade, and Daniel L. Thorek. "Abstract 6055: Cell confluence growth tracking as a supplement assay for clonogenic assay in prostate cancer radiotherapy combination evaluation." Cancer Research 82, no. 12_Supplement (2022): 6055. http://dx.doi.org/10.1158/1538-7445.am2022-6055.

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Abstract Background: Radiation therapy is a pillar of cancer care, and over 50% of all cancer patients will receive a form of this treatment. There is great interest in the capacity to improve radiation response and to de-escalate dose through combination pharmacological strategies. Evaluating these methods is a persistent challenge to the field. The clonogenic assay has been the gold standard assay in radiobiology research to evaluate the ability of a single cell to grow into a colony after different radiation treatments. However, the assay is laborious, time consuming and sensitive to enviro

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10

Kessel, Sarah, Scott Cribbes, Olivier Déry, et al. "High-Throughput 3D Tumor Spheroid Screening Method for Cancer Drug Discovery Using Celigo Image Cytometry." SLAS TECHNOLOGY: Translating Life Sciences Innovation 22, no. 4 (2016): 454–65. http://dx.doi.org/10.1177/2211068216652846.

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Oncologists have investigated the effect of protein or chemical-based compounds on cancer cells to identify potential drug candidates. Traditionally, the growth inhibitory and cytotoxic effects of the drugs are first measured in 2D in vitro models, and then further tested in 3D xenograft in vivo models. Although the drug candidates can demonstrate promising inhibitory or cytotoxicity results in a 2D environment, similar effects may not be observed under a 3D environment. In this work, we developed an image-based high-throughput screening method for 3D tumor spheroids using the Celigo image cyt

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Conference papers on the topic "Celigo image cytometer"

1

Chan, Leo L., Lucas Ferrari De Andrade, Charles A. Thomas, Dmitry Kuksin, and Kai Wucherpfennig. "Abstract 2325: Long term time-course monitoring of NK cell-mediated ADCC using the Celigo Image Cytometer." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.sabcs18-2325.

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Chan, Leo L., Lucas Ferrari De Andrade, Charles A. Thomas, Dmitry Kuksin, and Kai Wucherpfennig. "Abstract 2325: Long term time-course monitoring of NK cell-mediated ADCC using the Celigo Image Cytometer." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.am2019-2325.

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3

Chan, Leo L., Haohai Zhang, William Rice, et al. "Abstract 5774: Novel cell-based high-throughput hybridoma screening method using the Celigo image cytometer for antibody discovery." In Proceedings: AACR Annual Meeting 2018; April 14-18, 2018; Chicago, IL. American Association for Cancer Research, 2018. http://dx.doi.org/10.1158/1538-7445.am2018-5774.

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