Log in

Relevant bibliographies by topics / Cell assembly process / Journal articles

To see the other types of publications on this topic, follow the link: Cell assembly process.

Journal articles on the topic 'Cell assembly process'

Author: Grafiati

Published: 30 June 2021

Last updated: 29 July 2025

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Cell assembly process.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Sivia, Sebenova, Katarina Krajcova, and Velisek Karol. "Sensors in the Subsystems of Intelligent Assembly Cell." Applied Mechanics and Materials 220-223 (November 2012): 1825–28. http://dx.doi.org/10.4028/www.scientific.net/amm.220-223.1825.

Full text

Abstract:

Intelligent assembly cell conception includes new solution kind of how to create structures of automated and flexible assembly system. Intelligent behavior of the system as the control system will repose on monitoring of important parameters of the system in the real time. The designed automation sensory equipment provides for automatic monitoring of all automated equipment motions in the first case and in the second important level is important to obtain the information about the status, presence and character of the assembled objects or assembly process. The application of the sensory equipm

APA, Harvard, Vancouver, ISO, and other styles

2

Vetríková, Nina, and Michala Šimúnová. "Algorithms and Evolution Diagrams Application for Determining the New Assembly Process Sequences." Applied Mechanics and Materials 693 (December 2014): 16–21. http://dx.doi.org/10.4028/www.scientific.net/amm.693.16.

Full text

Abstract:

In this contribution is presented application of evolution diagrams at the assembly process of at the intelligent manufacturing and assembly cell. At this assembly cell was designed new assembly configuration for next research to our department and institute. Intelligent manufacturing and assembly cell is situated at the Institute of Production Systems and Applied Mechanics. The complex design of assembly sequences at the intelligent manufacturing and assembly cell is realized on the basis of evolution diagrams and outgoing from knowledge about intelligent manufacturing and assembly systems. F

APA, Harvard, Vancouver, ISO, and other styles

3

Parker, Scott D., and Eric Hunter. "A Cell-Line-Specific Defect in the Intracellular Transport and Release of Assembled Retroviral Capsids." Journal of Virology 74, no. 2 (2000): 784–95. http://dx.doi.org/10.1128/jvi.74.2.784-795.2000.

Full text

Abstract:

ABSTRACT Retrovirus assembly involves a complex series of events in which a large number of proteins must be targeted to a point on the plasma membrane where immature viruses bud from the cell. Gag polyproteins of most retroviruses assemble an immature capsid on the cytoplasmic side of the plasma membrane during the budding process (C-type assembly), but a few assemble immature capsids deep in the cytoplasm and are then transported to the plasma membrane (B- or D-type assembly), where they are enveloped. With both assembly phenotypes, Gag polyproteins must be transported to the site of viral b

APA, Harvard, Vancouver, ISO, and other styles

4

Ružarovský, Roman, Nina Danišová, and Karol Velíšek. "Sensory System Design as an Implement for the Development of the Intelligent Assembly Cell." Advanced Materials Research 628 (December 2012): 287–91. http://dx.doi.org/10.4028/www.scientific.net/amr.628.287.

Full text

Abstract:

Intelligent assembly cell conception includes new solution kind of how to create structures of automated and flexible assembly system. Intelligent behavior of the system as the control system will repose on monitoring of important parameters of the system in the real time. The designed automation sensory equipment provides for automatic monitoring of all automated equipment motions in the first case and in the second important level is important to obtain the information about the status, presence and character of the assembled objects or assembly process. The application of the sensory equipm

APA, Harvard, Vancouver, ISO, and other styles

5

Rónai, László, and Tamás Szabó. "Stability Analysis of an Assembly Process Using Simulation." Acta Technica Jaurinensis 13, no. 1 (2020): 14–24. http://dx.doi.org/10.14513/actatechjaur.v13.n1.531.

Full text

Abstract:

This paper deals with an assembly process of batteries with cell holder. The operation involves snap-fitting phenomenon, which is a mechanical stability problem. The structure of the cell holder is modelled with 2D flexible beam elements assuming large displacements. The stability of the equilibrium is investigated taking into consideration non-frictional and Coulomb frictional contacts. The goal of the analysis to determine the boundary point of the feed-motion from which the battery snaps-in to the final assembled position autonomously. The effect of the velocity of the battery feed-motion i

APA, Harvard, Vancouver, ISO, and other styles

6

Koch, G. L., C. Booth, and F. B. Wooding. "Dissociation and re-assembly of the endoplasmic reticulum in live cells." Journal of Cell Science 91, no. 4 (1988): 511–22. http://dx.doi.org/10.1242/jcs.91.4.511.

Full text

Abstract:

The endoplasmic reticulum (ER) of a typical interphase 3T3 fibroblast consists of a compact perinuclear arrangement of cisternae and lamellae which can be observed by immunofluorescence with anti-endoplasmin. During mitosis the reticulum dissociates into small fragments from which it appears to re-assemble in the daughter cells. When interphase 3T3 cells are exposed to calcium ionophores, but not other ionophores, there is a similar dissociation of the ER into small uniform fragments, which are dispersed throughout the cytoplasm. Electron microscopy shows that the fragments consist of small ve

APA, Harvard, Vancouver, ISO, and other styles

7

Connolly, Amy A., Kenji Sugioka, Chien-Hui Chuang, Joshua B. Lowry, and Bruce Bowerman. "KLP-7 acts through the Ndc80 complex to limit pole number in C. elegans oocyte meiotic spindle assembly." Journal of Cell Biology 210, no. 6 (2015): 917–32. http://dx.doi.org/10.1083/jcb.201412010.

Full text

Abstract:

During oocyte meiotic cell division in many animals, bipolar spindles assemble in the absence of centrosomes, but the mechanisms that restrict pole assembly to a bipolar state are unknown. We show that KLP-7, the single mitotic centromere–associated kinesin (MCAK)/kinesin-13 in Caenorhabditis elegans, is required for bipolar oocyte meiotic spindle assembly. In klp-7(−) mutants, extra microtubules accumulated, extra functional spindle poles assembled, and chromosomes frequently segregated as three distinct masses during meiosis I anaphase. Moreover, reducing KLP-7 function in monopolar klp-18(−

APA, Harvard, Vancouver, ISO, and other styles

8

Mao, Yong, and Jean E. Schwarzbauer. "Fibronectin fibrillogenesis, a cell-mediated matrix assembly process." Matrix Biology 24, no. 6 (2005): 389–99. http://dx.doi.org/10.1016/j.matbio.2005.06.008.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Palm, R. "Interaction Process Models in a Robotized Assembly Cell." IFAC Proceedings Volumes 22, no. 10 (1989): 385–90. http://dx.doi.org/10.1016/s1474-6670(17)53204-8.

Full text

APA, Harvard, Vancouver, ISO, and other styles

10

Peng, Lin Fa, Dian Kai Qiu, Pei Yun Yi, and Xin Min Lai. "Investigation of Thermal Influence on the Assembly of Polymer Electrolyte Membrane Fuel Cell Stacks." Advanced Materials Research 512-515 (May 2012): 1509–14. http://dx.doi.org/10.4028/www.scientific.net/amr.512-515.1509.

Full text

Abstract:

The assembly force in a proton exchange membrane fuel cell (PEMFC) stack affects the characteristics of the porosity and electrical conductivity. Generally, the stack is assembled at room temperature while it’s operated at about 80 °Cor even higher. As a result, the assembly pressure can’t keep constant due to thermal expansion. This paper focuses on the contact pressure between membrane electrode assembly (MEA) and bipolar plates in real operations. A three-dimensional finite element (FE) model for the assembly process is established with coupled thermal-mechanical effects. The discipline of

APA, Harvard, Vancouver, ISO, and other styles

11

Bareth, Bettina, Miroslav Nikolov, Isotta Lorenzi, et al. "Oms1 associates with cytochrome c oxidase assembly intermediates to stabilize newly synthesized Cox1." Molecular Biology of the Cell 27, no. 10 (2016): 1570–80. http://dx.doi.org/10.1091/mbc.e15-12-0811.

Full text

Abstract:

The mitochondrial cytochrome c oxidase assembles in the inner membrane from subunits of dual genetic origin. The assembly process of the enzyme is initiated by membrane insertion of the mitochondria-encoded Cox1 subunit. During complex maturation, transient assembly intermediates, consisting of structural subunits and specialized chaperone-like assembly factors, are formed. In addition, cofactors such as heme and copper have to be inserted into the nascent complex. To regulate the assembly process, the availability of Cox1 is under control of a regulatory feedback cycle in which translation of

APA, Harvard, Vancouver, ISO, and other styles

12

Liu, Hai Xia, Sheng Jie Li, Feng Lin, and Yong Nian Yan. "Modified Gelatin-Based Cell Assembling Process Using Glycerin." Advanced Materials Research 476-478 (February 2012): 443–47. http://dx.doi.org/10.4028/www.scientific.net/amr.476-478.443.

Full text

Abstract:

Cell assembly technology adopting the gelatin-based composite materials has found broad application in the field of disease mechanism research, drug development and organ reconstruction etc. But there are still several troublesome problems, such as the necessaries of high forming concentration of gelatin-based materials and the cell damage produced during extrusion. In view of existing situation, a modified gelatin-based cell assembling process using glycerin was brought forward. The results showed that adding 10% (v/v) glycerin to the existing gelatin-based composite materials, the cells inac

APA, Harvard, Vancouver, ISO, and other styles

13

Sakalian, Michael, and Eric Hunter. "Separate Assembly and Transport Domains within the Gag Precursor of Mason-Pfizer Monkey Virus." Journal of Virology 73, no. 10 (1999): 8073–82. http://dx.doi.org/10.1128/jvi.73.10.8073-8082.1999.

Full text

Abstract:

ABSTRACT Mason-Pfizer monkey virus (M-PMV), the prototypical type D retrovirus, assembles immature capsids within the cytoplasm of the cell prior to plasma membrane interaction. Several mutants of M-PMV Gag have been described which display altered transport, assembly, or both. In this report, we describe the use of an in vitro synthesis and assembly system to distinguish between defects in intracellular transport and the process of assembly itself for two previously describedgag gene mutants. Matrix domain mutant R55W converts the type D morphogenesis of M-PMV particles into type C and has be

APA, Harvard, Vancouver, ISO, and other styles

14

Khmelinskii, Anton, Clare Lawrence, Johanna Roostalu, and Elmar Schiebel. "Cdc14-regulated midzone assembly controls anaphase B." Journal of Cell Biology 177, no. 6 (2007): 981–93. http://dx.doi.org/10.1083/jcb.200702145.

Full text

Abstract:

Spindle elongation in anaphase of mitosis is a cell cycle–regulated process that requires coordination between polymerization, cross-linking, and sliding of microtubules (MTs). Proteins that assemble at the spindle midzone may be important for this process. In this study, we show that Ase1 and the separase–Slk19 complex drive midzone assembly in yeast. Whereas the conserved MT-bundling protein Ase1 establishes a midzone, separase–Slk19 is required to focus and center midzone components. An important step leading to spindle midzone assembly is the dephosphorylation of Ase1 by the protein phosph

APA, Harvard, Vancouver, ISO, and other styles

15

Steen, Rikke L., and Philippe Collas. "Mistargeting of B-Type Lamins at the End of Mitosis." Journal of Cell Biology 153, no. 3 (2001): 621–26. http://dx.doi.org/10.1083/jcb.153.3.621.

Full text

Abstract:

We previously showed that targeting of protein phosphatase 1 (PP1) to the nuclear envelope (NE) by the A-kinase anchoring protein, AKAP149, correlates with nuclear assembly of B-type lamins in vitro. We demonstrate here that failure of AKAP149-mediated assembly of B-type lamins into the nuclear lamina at the end of mitosis is followed by apoptosis, and induces expression of the gene encoding A-type lamins in cells that normally do not express lamins A/C. In HeLa cells, inhibition of PP1 association with the NE mediated by a peptide containing the PP1-binding domain of AKAP149 results in failur

APA, Harvard, Vancouver, ISO, and other styles

16

McGill, Melanie A., R. F. Andrew McKinley, and Tony J. C. Harris. "Independent cadherin–catenin and Bazooka clusters interact to assemble adherens junctions." Journal of Cell Biology 185, no. 5 (2009): 787–96. http://dx.doi.org/10.1083/jcb.200812146.

Full text

Abstract:

Proper epithelial structure requires adherens junction (AJ) assembly. In the early Drosophila embryo, AJ assembly depends on Bazooka (Baz; PAR-3), but it is unclear how Baz affects AJ assembly and what precursors are involved. To understand this process at the molecular level, we counted the number of core AJ proteins and Baz proteins at an average spot AJ (SAJ) and determined their dynamics with fluorescence recovery after photobleaching experiments. These data reveal that SAJs are subdivided into Baz clusters and cadherin–catenin clusters with independent protein numbers and dynamics. This i

APA, Harvard, Vancouver, ISO, and other styles

17

Davies, Alexander E., and Kenneth B. Kaplan. "Hsp90–Sgt1 and Skp1 target human Mis12 complexes to ensure efficient formation of kinetochore–microtubule binding sites." Journal of Cell Biology 189, no. 2 (2010): 261–74. http://dx.doi.org/10.1083/jcb.200910036.

Full text

Abstract:

The formation of functional kinetochores requires the accurate assembly of a large number of protein complexes. The Hsp90–Sgt1 chaperone complex is important for this process; however, its targets are not conserved and its exact contribution to kinetochore assembly is unclear. Here, we show that human Hsp90–Sgt1 interacts with the Mis12 complex, a so-called keystone complex required to assemble a large fraction of the kinetochore. Inhibition of Hsp90 or Sgt1 destabilizes the Mis12 complex and delays proper chromosome alignment due to inefficient formation of microtubule-binding sites. Interest

APA, Harvard, Vancouver, ISO, and other styles

18

Kleffe, Jürgen, Robert Weißmann, and Florian F. Schmitzberger. "Single Nucleotide Polymorphisms Caused by Assembly Errors." Genomics Insights 3 (January 2010): GEI.S3653. http://dx.doi.org/10.4137/gei.s3653.

Full text

Abstract:

We compare the results of three different assembler programs, Celera, Phrap and Mira2, for the same set of about a hundred thousand Sanger reads derived from an unknown bacterial genome. In difference to previous assembly comparisons we do not focus on speed of computation and numbers of assembled contigs but on how the different sequence assemblies agree by content. Threefold consistently assembled genome regions are identified in order to estimate a lower bound of erroneously identified single nucleotide polymorphisms (SNP) caused by nothing but the process of mathematical sequence assembly.

APA, Harvard, Vancouver, ISO, and other styles

19

Agnetis, A., R. Macchiaroli, and F. Nicolò. "Optimization of the Assembly Process in a Flexible Cell." IFAC Proceedings Volumes 29, no. 1 (1996): 613–18. http://dx.doi.org/10.1016/s1474-6670(17)57730-7.

Full text

APA, Harvard, Vancouver, ISO, and other styles

20

Mozdy, A. D., J. M. McCaffery, and J. M. Shaw. "Dnm1p Gtpase-Mediated Mitochondrial Fission Is a Multi-Step Process Requiring the Novel Integral Membrane Component Fis1p." Journal of Cell Biology 151, no. 2 (2000): 367–80. http://dx.doi.org/10.1083/jcb.151.2.367.

Full text

Abstract:

Yeast Dnm1p is a soluble, dynamin-related GTPase that assembles on the outer mitochondrial membrane at sites where organelle division occurs. Although these Dnm1p-containing complexes are thought to trigger constriction and fission, little is known about their composition and assembly, and molecules required for their membrane recruitment have not been isolated. Using a genetic approach, we identified two new genes in the fission pathway, FIS1 and FIS2. FIS1 encodes a novel, outer mitochondrial membrane protein with its amino terminus exposed to the cytoplasm. Fis1p is the first integral membr

APA, Harvard, Vancouver, ISO, and other styles

21

Rabito, C. A., J. A. Jarrell, and J. A. Scott. "Gap junctions and synchronization of polarization process during epithelial reorganization." American Journal of Physiology-Cell Physiology 253, no. 2 (1987): C329—C336. http://dx.doi.org/10.1152/ajpcell.1987.253.2.c329.

Full text

Abstract:

The formation of intercellular communication during epithelial reorganization of LLC-PK1 cells was studied with a new, nondisruptive flow cytometric technique. The assembly of these junctions, as demonstrated by cell-to-cell transfer of the fluorescent dye carboxy dimethyl fluorescein, occurs very early during epithelial reorganization. Close cell-to-cell interaction is required for the assembly to occur. Low temperature, treatment with 10(-3) M ouabain, or treatment with the Ca2+ ionophore A23187 inhibits assembly of these junctions. Removal of Ca2+ from the incubation medium, on the other ha

APA, Harvard, Vancouver, ISO, and other styles

22

Jánoš, Rudolf, and Baňasová Antónia. "CELL DESIGN FOR GEARBOX ASSEMBLY." TECHNICAL SCIENCES AND TECHNOLOG IES, no. 3(13) (2018): 49–54. http://dx.doi.org/10.25140/2411-5363-2018-3(13)-49-54.

Full text

Abstract:

Urgency of the research. Automation is the next step in increasing productivity and performance. It represents the autonomous management of the entire system as well as the assembly or production process. This completely eliminates a human factor from the work process. Target setting. Today's robots and manipulators are now autonomous. Automated systems can be found in almost all industries. They are an indispensable part of non-productive environments, but we can also find them in non-industrial areas as well. Their dynamic development extends to service robotics. For their productivity, they

APA, Harvard, Vancouver, ISO, and other styles

23

Fowler, Devin, Vladimir Gurau, and Daniel Cox. "Bridging the Gap between Automated Manufacturing of Fuel Cell Components and Robotic Assembly of Fuel Cell Stacks." Energies 12, no. 19 (2019): 3604. http://dx.doi.org/10.3390/en12193604.

Full text

Abstract:

Recently demonstrated robotic assembling technologies for fuel cell stacks used fuel cell components manually pre-arranged in stacks (presenters). Identifying the original orientation of fuel cell components and loading them in presenters for a subsequent automated assembly process is a difficult, repetitive work cycle which if done manually, deceives the advantages offered by either the automated fabrication technologies for fuel cell components or by the robotic assembly processes. We present for the first time a robotic technology which enables the integration of automated fabrication proce

APA, Harvard, Vancouver, ISO, and other styles

24

Grobelny, Paweł, Lukasz Furmanski, Jolanta B. Krolczyk, and Stanisław Legutko. "Comparison of the Assembly Line and Cell Assembly - A Case Study in Mechanical Engineering Company." Applied Mechanics and Materials 809-810 (November 2015): 1331–36. http://dx.doi.org/10.4028/www.scientific.net/amm.809-810.1331.

Full text

Abstract:

The paper compares the assembly process of milling machine using two systems: cell system and production line which was introduced after the changes in the machine assembly system of the company. Assembly in both systems takes place in the SKD (semi-knocked-down) system, which consists of assembly finished, large parts of the machine (higher completeness units) imported into the country of installation. The illustrated example presents advantages and disadvantages of assembly of milling machines in two systems: cell assembly and on the assembly line. In this example, the employees in each cell

APA, Harvard, Vancouver, ISO, and other styles

25

Ott, David E., Lori V. Coren, Raymond C. Sowder, Julian Adams, and Ulrich Schubert. "Retroviruses Have Differing Requirements for Proteasome Function in the Budding Process." Journal of Virology 77, no. 6 (2003): 3384–93. http://dx.doi.org/10.1128/jvi.77.6.3384-3393.2003.

Full text

Abstract:

ABSTRACT Proteasome inhibitors reduce the budding of human immunodeficiency virus types 1 (HIV-1) and 2, simian immunodeficiency virus, and Rous sarcoma virus. To investigate this effect further, we examined the budding of other retroviruses from proteasome inhibitor-treated cells. The viruses tested differed in their Gag organization, late (L) domain usage, or assembly site from those previously examined. We found that proteasome inhibition decreased the budding of murine leukemia virus (plasma membrane assembly, PPPY L domain) and Mason-Pfizer monkey virus (cytoplasmic assembly, PPPY L domai

APA, Harvard, Vancouver, ISO, and other styles

26

Redick, S. D., and J. E. Schwarzbauer. "Rapid intracellular assembly of tenascin hexabrachions suggests a novel cotranslational process." Journal of Cell Science 108, no. 4 (1995): 1761–69. http://dx.doi.org/10.1242/jcs.108.4.1761.

Full text

Abstract:

Tenascin, an extracellular matrix protein that modulates cell adhesion, exists as a unique six-armed structure called a hexabrachion. The human hexabrachion is composed of six identical 320 kDa subunits and the structure is stabilized by inter-subunit disulfide bonds between amino-terminal segments. We have examined the biosynthesis of tenascin and its assembly into hexabrachions using pulsechase labeling of U-138 MG human glioma cells. Newly synthesized tenascin hexamers are secreted within 60 minutes of translation initiation. Intracellularly, as early as full length tenascin can be detected

APA, Harvard, Vancouver, ISO, and other styles

27

Féral, Chloé C., Andries Zijlstra, Eugene Tkachenko, et al. "CD98hc (SLC3A2) participates in fibronectin matrix assembly by mediating integrin signaling." Journal of Cell Biology 178, no. 4 (2007): 701–11. http://dx.doi.org/10.1083/jcb.200705090.

Full text

Abstract:

Integrin-dependent assembly of the fibronectin (Fn) matrix plays a central role in vertebrate development. We identify CD98hc, a membrane protein, as an important component of the matrix assembly machinery both in vitro and in vivo. CD98hc was not required for biosynthesis of cellular Fn or the maintenance of the repertoire or affinity of cellular Fn binding integrins, which are important contributors to Fn assembly. Instead, CD98hc was involved in the cell's ability to exert force on the matrix and did so by dint of its capacity to interact with integrins to support downstream signals that le

APA, Harvard, Vancouver, ISO, and other styles

28

Javorova, Angela, Erika Hrušková, and Karol Velíšek. "Designing of Assembly Cell by CA System Support." Key Engineering Materials 467-469 (February 2011): 2060–65. http://dx.doi.org/10.4028/www.scientific.net/kem.467-469.2060.

Full text

Abstract:

This paper deals with a designing process of assembly cell using CA systems. Methodology solved in the paper is applying CA tools. The methodology is divided in to five main project phases: assembly product analysis, hardware specification, selection of proper control system, control system simulation and whole process simulation.

APA, Harvard, Vancouver, ISO, and other styles

29

Rollenhagen, Christiane, and Nelly Panté. "Nuclear import of spliceosomal snRNPsThis paper is one of a selection of papers published in this Special Issue, entitled The Nucleus: A Cell Within A Cell." Canadian Journal of Physiology and Pharmacology 84, no. 3-4 (2006): 367–76. http://dx.doi.org/10.1139/y05-101.

Full text

Abstract:

Uridine-rich small nuclear ribonucleoproteins (U snRNPs) are the building units of the spliceosome. These RNA and protein complexes assemble in the cytoplasm. After proper assembly and RNA maturation, mature U snRNPs are imported into the cell nucleus, where they take part in the splicing process. In this paper we review the current knowledge of how U snRNPs enter the nucleus.

APA, Harvard, Vancouver, ISO, and other styles

30

WANG, XIAOFEI, ALESSANDRO IANNACCONE, and MONICA M. JABLONSKI. "Contribution of Müller cells toward the regulation of photoreceptor outer segment assembly." Neuron Glia Biology 1, no. 3 (2004): 291–96. http://dx.doi.org/10.1017/s1740925x05000049.

Full text

Abstract:

The assembly of photoreceptor outer segments into stacked discs is a complicated process, the precise regulation of which remains a mystery. It is known that the integrity of the outer segment is heavily dependent upon surrounding cell types including the retinal pigment epithelium and Müller cells; however the role played by Müller cells within this photoreceptor-specific process has not been fully explored. Using an RPE-deprived but otherwise intact Xenopus laevis eye rudiment preparation, we reveal that Müller cell involvement in outer segment assembly is dependent upon the stimulus provide

APA, Harvard, Vancouver, ISO, and other styles

31

Rahman, Sheikh Abdul, Peter Koch, Julian Weichsel, et al. "Investigating the Role of F-Actin in Human Immunodeficiency Virus Assembly by Live-Cell Microscopy." Journal of Virology 88, no. 14 (2014): 7904–14. http://dx.doi.org/10.1128/jvi.00431-14.

Full text

Abstract:

ABSTRACTHuman immunodeficiency virus type 1 (HIV-1) particles assemble at the plasma membrane, which is lined by a dense network of filamentous actin (F-actin). Large amounts of actin have been detected in HIV-1 virions, proposed to be incorporated by interactions with the nucleocapsid domain of the viral polyprotein Gag. Previous studies addressing the role of F-actin in HIV-1 particle formation using F-actin-interfering drugs did not yield consistent results. Filamentous structures pointing toward nascent HIV-1 budding sites, detected by cryo-electron tomography and atomic force microscopy,

APA, Harvard, Vancouver, ISO, and other styles

32

Hill, Sam R., Reidun Twarock, and Eric C. Dykeman. "The impact of local assembly rules on RNA packaging in a T = 1 satellite plant virus." PLOS Computational Biology 17, no. 8 (2021): e1009306. http://dx.doi.org/10.1371/journal.pcbi.1009306.

Full text

Abstract:

The vast majority of viruses consist of a nucleic acid surrounded by a protective icosahedral protein shell called the capsid. During viral infection of a host cell, the timing and efficiency of the assembly process is important for ensuring the production of infectious new progeny virus particles. In the class of single-stranded RNA (ssRNA) viruses, the assembly of the capsid takes place in tandem with packaging of the ssRNA genome in a highly cooperative co-assembly process. In simple ssRNA viruses such as the bacteriophage MS2 and small RNA plant viruses such as STNV, this cooperative proce

APA, Harvard, Vancouver, ISO, and other styles

33

Lalitha, Krishnamoorthy, and Subbiah Nagarajan. "Strongly fluorescent organogels and self-assembled nanostructures from pyrene coupled coumarin derivatives: application in cell imaging." Journal of Materials Chemistry B 3, no. 28 (2015): 5690–701. http://dx.doi.org/10.1039/c5tb00694e.

Full text

Abstract:

The present work reports facile synthesis of pyrene coupled coumarin derivatives which could form self-assembled molecular gel and nano-flakes. The nanomaterials obtained via a self-assembly process could be potentially used in fluorescence imaging applications.

APA, Harvard, Vancouver, ISO, and other styles

34

Kmiec, Eric B., JoAnn M. Sekiguchi, and Allyson D. Cole. "Studies on the ATP requirements of in vitro chromatin assembly." Biochemistry and Cell Biology 67, no. 8 (1989): 443–54. http://dx.doi.org/10.1139/o89-070.

Full text

Abstract:

To gain a more complete understanding of the process of in vitro chromatin assembly, an examination of the energy requirements of nucleosome formation must be undertaken. The experiments outlined in this manuscript address this issue by making use of the Xenopus laevis S-150 cell-free extract. The S-150 catalyzes chromatin assembly on circular DNA templates dependent either on the exogenous addition of ATP or regeneration of endogenous ATP. We define two distinct, but temporally ordered, phases of the overall process. The first, nucleosome formation, occurs in the presence of endogenous levels

APA, Harvard, Vancouver, ISO, and other styles

35

Wu, Jiang, Quan Wei Zhang, Dong Lai, Jia Fa Liao, and Chun Lan Jia. "Research on Cell Production Layout of TV Assembly Line." Advanced Materials Research 926-930 (May 2014): 818–21. http://dx.doi.org/10.4028/www.scientific.net/amr.926-930.818.

Full text

Abstract:

Traditional TV assembly line production mode faced with the problem of low productivity in response to the varieties of small batch orders. The paper studies Cell production mode, combined with television assembly process characteristics, to explore the Cell production layout mode that is suitable for TV assembly line.

APA, Harvard, Vancouver, ISO, and other styles

36

Cobbold, Christian, Miriam Windsor, James Parsley, Ben Baldwin, and Thomas Wileman. "Reduced redox potential of the cytosol is important for African swine fever virus capsid assembly and maturation." Journal of General Virology 88, no. 1 (2007): 77–85. http://dx.doi.org/10.1099/vir.0.82257-0.

Full text

Abstract:

Assembly of African swine fever virus (ASFV) involves the transfer of the major capsid protein, p73, from the cytosol onto the cytoplasmic face of endoplasmic reticulum-derived membranes. During this process, the folding of p73 is dependent upon transient association with a specific viral chaperone, CAP80. The cell cytoplasm maintains high concentrations of reduced glutathione, leading to a reducing environment. Here, the effects of redox environment on the assembly of ASFV have been studied. Diamide, which oxidizes the cell cytosol, slowed the folding of p73 and prevented release from CAP80 a

APA, Harvard, Vancouver, ISO, and other styles

37

Brémaud, Erwan, Cyril Favard, and Delphine Muriaux. "Deciphering the Assembly of Enveloped Viruses Using Model Lipid Membranes." Membranes 12, no. 5 (2022): 441. http://dx.doi.org/10.3390/membranes12050441.

Full text

Abstract:

The cell plasma membrane is mainly composed of phospholipids, cholesterol and embedded proteins, presenting a complex interface with the environment. It maintains a barrier to control matter fluxes between the cell cytosol and its outer environment. Enveloped viruses are also surrounded by a lipidic membrane derived from the host-cell membrane and acquired while exiting the host cell during the assembly and budding steps of their viral cycle. Thus, model membranes composed of selected lipid mixtures mimicking plasma membrane properties are the tools of choice and were used to decipher the firs

APA, Harvard, Vancouver, ISO, and other styles

38

He, Hongjian, and Bing Xu. "Instructed-Assembly (iA): A Molecular Process for Controlling Cell Fate." Bulletin of the Chemical Society of Japan 91, no. 6 (2018): 900–906. http://dx.doi.org/10.1246/bcsj.20180038.

Full text

APA, Harvard, Vancouver, ISO, and other styles

39

Sechler, Jan L., Hongwei Rao, Anne Marie Cumiskey, et al. "A novel fibronectin binding site required for fibronectin fibril growth during matrix assembly." Journal of Cell Biology 154, no. 5 (2001): 1081–88. http://dx.doi.org/10.1083/jcb.200102034.

Full text

Abstract:

Fibronectin (FN) assembly into a fibrillar extracellular matrix is a stepwise process requiring participation from multiple FN domains. Fibril formation is regulated in part by segments within the first seven type III repeats (III1–7). To define the specific function(s) of this region, recombinant FNs (recFNs) containing an overlapping set of deletions were tested for the ability to assemble into fibrils. Surprisingly, recFN lacking type III repeat III1 (FNΔIII1), which contains a cryptic FN binding site and has been suggested to be essential for fibril assembly, formed a matrix identical in a

APA, Harvard, Vancouver, ISO, and other styles

40

Lu, Z. H., D. B. Sittman, D. T. Brown, R. Munshi, and G. H. Leno. "Histone H1 modulates DNA replication through multiple pathways in Xenopus egg extract." Journal of Cell Science 110, no. 21 (1997): 2745–58. http://dx.doi.org/10.1242/jcs.110.21.2745.

Full text

Abstract:

We investigated the effects of histone H1s on DNA replication using Xenopus egg extract. Mouse variants H1c and H10 were assembled onto Xenopus sperm chromatin by the extract during the remodeling that accompanies nuclear decondensation. The association of H1 with chromatin was rapid and concentration dependent. H1-associated chromatin displayed a typical nucleosomal repeat pattern indicating that linker histones are properly positioned along the DNA. The presence of H1 on sperm chromatin reduced both the rate and extent of DNA replication in egg extract. This reduction in rate is due, in part

APA, Harvard, Vancouver, ISO, and other styles

41

Coulombe, P. A., Y. M. Chan, K. Albers, and E. Fuchs. "Deletions in epidermal keratins leading to alterations in filament organization in vivo and in intermediate filament assembly in vitro." Journal of Cell Biology 111, no. 6 (1990): 3049–64. http://dx.doi.org/10.1083/jcb.111.6.3049.

Full text

Abstract:

To investigate the sequences important for assembly of keratins into 10-nm filaments, we used a combined approach of (a) transfection of mutant keratin cDNAs into epithelial cells in vivo, and (b) in vitro assembly of mutant and wild-type keratins. Keratin K14 mutants missing the nonhelical carboxy- and amino-terminal domains not only integrated without perturbation into endogenous keratin filament networks in vivo, but they also formed 10-nm filaments with K5 in vitro. Surprisingly, keratin mutants missing the highly conserved L L E G E sequence, common to all intermediate filament proteins a

APA, Harvard, Vancouver, ISO, and other styles

42

Jurczyk, Agata, Adam Gromley, Sambra Redick, et al. "Pericentrin forms a complex with intraflagellar transport proteins and polycystin-2 and is required for primary cilia assembly." Journal of Cell Biology 166, no. 5 (2004): 637–43. http://dx.doi.org/10.1083/jcb.200405023.

Full text

Abstract:

Primary cilia are nonmotile microtubule structures that assemble from basal bodies by a process called intraflagellar transport (IFT) and are associated with several human diseases. Here, we show that the centrosome protein pericentrin (Pcnt) colocalizes with IFT proteins to the base of primary and motile cilia. Immunogold electron microscopy demonstrates that Pcnt is on or near basal bodies at the base of cilia. Pcnt depletion by RNA interference disrupts basal body localization of IFT proteins and the cation channel polycystin-2 (PC2), and inhibits primary cilia assembly in human epithelial

APA, Harvard, Vancouver, ISO, and other styles

43

Li, Shaohua, David Harrison, Salvatore Carbonetto, et al. "Matrix assembly, regulation, and survival functions of laminin and its receptors in embryonic stem cell differentiation." Journal of Cell Biology 157, no. 7 (2002): 1279–90. http://dx.doi.org/10.1083/jcb.200203073.

Full text

Abstract:

Laminin-1 is essential for early embryonic basement membrane assembly and differentiation. Several steps can be distinguished, i.e., the expression of laminin and companion matrix components, their accumulation on the cell surface and assembly into basement membrane between endoderm and inner cell mass, and the ensuing differentiation of epiblast. In this study, we used differentiating embryoid bodies derived from mouse embryonic stem cells null for γ1-laminin, β1-integrin and α/β-dystroglycan to dissect the contributions of laminin domains and interacting receptors to this process. We found t

APA, Harvard, Vancouver, ISO, and other styles

44

Xu, Minjun, Xiaochuan Chen, Jun Wang, and Yong Li. "Finite element analysis modeling research on the compression process of cotton fiber assembly." Textile Research Journal 90, no. 11-12 (2019): 1414–27. http://dx.doi.org/10.1177/0040517519886558.

Full text

Abstract:

In order to analyze the compressive stress state of a cotton fiber assembly in the compression process, a new cotton fiber assembly model, the tetrakaidecahedral porous cotton fiber assembly model, is presented based on the idea of three-dimensional open-cell foam material modeling. Based on the model, the compression process of cotton fiber assembly is analyzed using the finite element method. The change in the compressive stress of the cotton fiber assembly in the compression process is successfully described. The measurement method of compressive modulus of the cotton fiber assembly is also

APA, Harvard, Vancouver, ISO, and other styles

45

Kim, Jane H., Amitava Mukherjee, Sethu M. Madhavan, Martha Konieczkowski, and John R. Sedor. "WT1-interacting protein (Wtip) regulates podocyte phenotype by cell-cell and cell-matrix contact reorganization." American Journal of Physiology-Renal Physiology 302, no. 1 (2012): F103—F115. http://dx.doi.org/10.1152/ajprenal.00419.2011.

Full text

Abstract:

Podocytes respond to environmental cues by remodeling their slit diaphragms and cell-matrix adhesive junctions. Wt1-interacting protein (Wtip), an Ajuba family LIM domain scaffold protein expressed in the podocyte, coordinates cell adhesion changes and transcriptional responses to regulate podocyte phenotypic plasticity. We evaluated effects of Wtip on podocyte cell-cell and cell-matrix contact organization using gain-of- and loss-of-function methods. Endogenous Wtip targeted to focal adhesions in adherent but isolated podocytes and then shifted to adherens junctions after cells made stable, h

APA, Harvard, Vancouver, ISO, and other styles

46

Sechler, J. L., Y. Takada, and J. E. Schwarzbauer. "Altered rate of fibronectin matrix assembly by deletion of the first type III repeats." Journal of Cell Biology 134, no. 2 (1996): 573–83. http://dx.doi.org/10.1083/jcb.134.2.573.

Full text

Abstract:

The assembly of fibronectin (FN) into a fibrillar matrix is a complex stepwise process that involves binding to integrin receptors as well as interactions between FN molecules. To follow the progression of matrix formation and determine the stages during which specific domains function, we have developed cell lines that lack an endogenous FN matrix but will form fibrils when provided with exogenous FN. Recombinant FNs (recFN) containing deletions of either the RGD cell-binding sequence (RGD-) or the first type III repeats (FN delta III1-7) including the III1 FN binding site were generated with

APA, Harvard, Vancouver, ISO, and other styles

47

JOHN, David C. A., та Neil J. BULLEID. "Intracellular dissociation and reassembly of prolyl 4-hydroxylase:the α-subunits associated with the immunoglobulin-heavy-chain binding protein (BiP) allowing reassembly with the β-subunit". Biochemical Journal 317, № 3 (1996): 659–65. http://dx.doi.org/10.1042/bj3170659.

Full text

Abstract:

Prolyl 4-hydroxylase (P4-H) consists of two distinct polypeptides; the catalytically more important α-subunit and the β-subunit, which is identical to the multifunctional enzyme protein disulphide isomerase. The enzyme appears to be assembled in vivo into an α2β2 tetramer from newly synthesized α-subunits associating with an endogenous pool of β-subunits. Using a cell-free system, we have shown previously that enzyme assembly is redox-dependent and that assembled α-subunits are intramolecularly disulphide-bonded [John and Bulleid (1994) Biochemistry 33, 14018–14025]. Here we have studied this

APA, Harvard, Vancouver, ISO, and other styles

48

Aldana, Maximino, Miguel Fuentes-Cabrera, and Martín Zumaya. "Self-Propulsion Enhances Polymerization." Entropy 22, no. 2 (2020): 251. http://dx.doi.org/10.3390/e22020251.

Full text

Abstract:

Self-assembly is a spontaneous process through which macroscopic structures are formed from basic microscopic constituents (e.g., molecules or colloids). By contrast, the formation of large biological molecules inside the cell (such as proteins or nucleic acids) is a process more akin to self-organization than to self-assembly, as it requires a constant supply of external energy. Recent studies have tried to merge self-assembly with self-organization by analyzing the assembly of self-propelled (or active) colloid-like particles whose motion is driven by a permanent source of energy. Here we pr

APA, Harvard, Vancouver, ISO, and other styles

49

Lévy, F., R. Larsson, and S. Kvist. "Translocation of peptides through microsomal membranes is a rapid process and promotes assembly of HLA-B27 heavy chain and beta 2-microglobulin translated in vitro." Journal of Cell Biology 115, no. 4 (1991): 959–70. http://dx.doi.org/10.1083/jcb.115.4.959.

Full text

Abstract:

We have translated major histocompatibility complex (MHC) class I heavy chains and human beta 2-microglobulin in vitro in the presence of microsomal membranes and a peptide from the nucleoprotein of influenza A. This peptide stimulates assembly of HLA-B27 heavy chain and beta 2-microglobulin about fivefold. By modifying this peptide to contain biotin at its amino terminus, we could precipitate HLA-B27 heavy chains with immobilized streptavidin, thereby directly demonstrating class I heavy chain-peptide association under close to physiological conditions. The biotin-modified peptide stimulates

APA, Harvard, Vancouver, ISO, and other styles

50

Duan, Feng, Masahiro Morioka, Jeffrey Too Chuan Tan, and Tamio Arai. "Multi-Modal Assembly-Support System for Cell Production." International Journal of Automation Technology 2, no. 5 (2008): 384–89. http://dx.doi.org/10.20965/ijat.2008.p0384.

Full text

Abstract:

Cell production systems meet the diversified production and quantity requirements flexibly. However, their efficiency mainly depends on the human operators' working performance. In order to improve the efficiency of the cell production system, effective assembly-support systems should be developed to assist human operators during the assembly process. In this paper, a multi-modal assembly-support system (MASS) is proposed, which aims to support assembly human operators from both information aspect and physical aspect while meets the actual manufacturing requirements. With the information and p

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!