Relevant bibliographies by topics / Cell-Based cytotoxicity assay

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  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Conference papers

Academic literature on the topic 'Cell-Based cytotoxicity assay'

Author: Grafiati
Published: 4 June 2021
Last updated: 3 February 2022

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Journal articles on the topic "Cell-Based cytotoxicity assay"

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Wakuri, S., K. Yamakage, Y. Kazuki, et al. "Correlation between luminescence intensity and cytotoxicity in cell-based cytotoxicity assay using luciferase." Analytical Biochemistry 522 (April 2017): 18–29. http://dx.doi.org/10.1016/j.ab.2017.01.015.

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Wong, Pamela, Julia A. Wagner, Melissa M. Berrien-Elliott, Timothy Schappe, and Todd A. Fehniger. "Flow cytometry-based ex vivo murine NK cell cytotoxicity assay." STAR Protocols 2, no. 1 (2021): 100262. http://dx.doi.org/10.1016/j.xpro.2020.100262.

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Martinez, Emily M., Samuel D. Klebanoff, Stephanie Secrest, et al. "High-Throughput Flow Cytometric Method for the Simultaneous Measurement of CAR-T Cell Characterization and Cytotoxicity against Solid Tumor Cell Lines." SLAS DISCOVERY: Advancing the Science of Drug Discovery 23, no. 7 (2018): 603–12. http://dx.doi.org/10.1177/2472555218768745.

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High-throughput flow cytometry is an attractive platform for the analysis of adoptive cellular therapies such as chimeric antigen receptor T cell therapy (CAR-T) because it allows for the concurrent measurement of T cell–dependent cellular cytotoxicity (TDCC) and the functional characterization of engineered T cells with respect to percentage of CAR transduction, T cell phenotype, and measurement of T cell function such as activation in a single assay. The use of adherent tumor cell lines can be challenging in these flow-based assays. Here, we present the development of a high-throughput flow-
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Nazarian, Aaron A., Ivonne L. Archibeque, Yen H. Nguyen, Paul Wang, Angus M. Sinclair, and David A. Powers. "Characterization of Bispecific T-cell Engager (BiTE®) Antibodies with a High-Capacity T-cell Dependent Cellular Cytotoxicity (TDCC) Assay." Journal of Biomolecular Screening 20, no. 4 (2014): 519–27. http://dx.doi.org/10.1177/1087057114561405.

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The Bispecific T-cell Engager (BiTE®) antibody modality is a clinically validated immunotherapeutic approach for targeting tumors. Using T-cell dependent cellular cytotoxicity (TDCC) assays, we measure the percentage of specific cytotoxicity induced when a BiTE molecule engages T-cells, redirects T-cell mediated cytolysis, and ultimately kills target cells. We establish a novel luminescence-based TDCC assay quantified by measuring cell viability via constitutive expression of luciferase. The luciferase-based TDCC assay performance is valid and comparable to an adenosine triphosphate (ATP)-base
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Antona, Silvia, Tobias Abele, Kevin Jahnke, et al. "Droplet‐Based Combinatorial Assay for Cell Cytotoxicity and Cytokine Release Evaluation." Advanced Functional Materials 30, no. 46 (2020): 2003479. http://dx.doi.org/10.1002/adfm.202003479.

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Ramis, G., L. Martínez-Alarcón, J. J. Quereda, et al. "Optimization of cytotoxicity assay by real-time, impedance-based cell analysis." Biomedical Microdevices 15, no. 6 (2013): 985–95. http://dx.doi.org/10.1007/s10544-013-9790-8.

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Meindl, Claudia, Markus Absenger, Eva Roblegg, and Eleonore Fröhlich. "Suitability of Cell-Based Label-Free Detection for Cytotoxicity Screening of Carbon Nanotubes." BioMed Research International 2013 (2013): 1–13. http://dx.doi.org/10.1155/2013/564804.

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Cytotoxicity testing of nanoparticles (NPs) by conventional screening assays is often complicated by interference. Carbon nanotubes (CNTs) are particularly difficult to assess. To test the suitability of cell-based label-free techniques for this application, a panel of CNTs with different diameters and surface functionalizations was assessed by impedance-based technique (xCELLigenceRTCA) and automated microscopy (Cell-IQ) compared to formazan bioreduction (MTS assay). For validation of the label-free systems different concentrations of ethanol and of amine (AMI) polystyrene NPs were used. CNTs
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Fukushima, Toshiro, Hitomi Tanaka, and Takeshi Yamamoto. "Comparative Study of Cigarette Smoke Cytotoxicity Using Two In Vitro Assay Systems." Beiträge zur Tabakforschung International/Contributions to Tobacco Research 26, no. 3 (2014): 98–108. http://dx.doi.org/10.2478/cttr-2014-0013.

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SUMMARYThe aim of this study was to compare the results obtained from two in vitro cytotoxicity assays that depend upon different mechanisms/modes of action. The Neutral Red Uptake (NRU) assay is based on endocytotic activity whereas the Water Soluble Tetrazolium Salts (WST-1) assay is based on mitochondrial dehydrogenase activity. Both were investigated in light of their wide use and documented validation. The total particulate matter (TPM) and gas vapor phase (GVP) of main stream smoke derived from Kentucky reference cigarettes 3R4F and 10 test cigarettes made of 100% flue-cured or 100% Burl
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Park, Ki-Hyun, Hyesun Park, Myungshin Kim, Yonggoo Kim, Kyungja Han, and Eun-Jee Oh. "Evaluation of NK Cell Function by Flowcytometric Measurement and Impedance Based Assay Using Real-Time Cell Electronic Sensing System." BioMed Research International 2013 (2013): 1–10. http://dx.doi.org/10.1155/2013/210726.

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Although real-time cell electronic sensing (RT-CES) system-based natural killer (NK) cytotoxicity has been introduced, it has not been evaluated using human blood samples. In present study, we measured flowcytometry based assay (FCA) and RT-CES based NK cytotoxicity and analyzed degranulation activity (CD107a) and cytokine production. In 98 healthy individuals, FCA with peripheral blood mononuclear cells (PBMCs) at effector to target (E/T) ratio of 32 revealed 46.5 ± 2.6% cytolysis of K562 cells, and 23.5 ± 1.1% of NK cells showed increased degranulation. In RT-CES system, adherent NIH3T3 targ
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Wilkinson, Robert W., Alice E. Lee-MacAry, Derek Davies, David Snary, and Elizabeth L. Ross. "Antibody-dependent cell-mediated cytotoxicity: a flow cytometry-based assay using fluorophores." Journal of Immunological Methods 258, no. 1-2 (2001): 183–91. http://dx.doi.org/10.1016/s0022-1759(01)00474-4.

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Dissertations / Theses on the topic "Cell-Based cytotoxicity assay"

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Todaro, Biagio. "Ingénierie de systèmes biomoléculaires pour l’immunothérapie anti-tumorale." Thesis, Université Grenoble Alpes, 2020. http://www.theses.fr/2020GRALV036.

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Les "Antibody Recruiting Molecules" (ARMs) font partie des pistes prometteuses en immunothérapie contre le cancer et les pathogènes. Dans le contexte tumoral, un ARM a la capacité de relier la cellule cible et des composants du système immunitaire pour conduire à une réponse cytotoxique. Dans ce travail de thèse, nous avons décrit la conception et la synthèse par chimie click de plusieurs ARMs capables de cibler des protéines surexprimées à la surface des cellules cancéreuses par de "tumoral binding modules" (TBMs) basés sur des peptides et d’engager des anticorps endogènes naturels par l’inte
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Linares, Irwin Alexander Patiño. "Estudos citotóxicos de moléculas antitumorais e antiparasitárias em células de câncer de fígado (HepG2) e de fibroblasto de hamster (V79-4)." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/75/75133/tde-27112013-142548/.

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Os ensaios celulares têm ganhado relevância na gênese planejada de fármacos, devido a sua utilização nas diversas etapas envolvidas neste processo. Estes ensaios envolvem a caracterização da atividade farmacológica, propriedades farmacocinéticas e atividade tóxica para compreender a atividade biológica das moléculas de interesse. Neste trabalho, os ensaios celulares foram usados para identificar a atividade anticancerígena e a atividade tóxica de moléculas, uma vez que a morte celular é o parâmetro avaliado em ambos os casos. No presente estudo foram avaliados 34 compostos, sendo dezessete mol
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Book chapters on the topic "Cell-Based cytotoxicity assay"

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Larson, Brad, Lubna Hussain, and Jenny Schroeder. "Validation of an Image-Based 3D Natural Killer Cell-Mediated Cytotoxicity Assay." In Methods in Pharmacology and Toxicology. Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0171-6_7.

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Salinas-Jazmín, Nohemí, Emiliano Hisaki-Itaya, and Marco A. Velasco-Velázquez. "A Flow Cytometry-Based Assay for the Evaluation of Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) in Cancer Cells." In Methods in Molecular Biology. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-0856-1_16.

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Dostert, G., V. Jouan-Hureaux, H. Louis, and É. Velot. "Umbilical Mesenchymal Stem Cell-Derived Extracellular Vesicle Conditioning Has an Immunosuppressive Effect on NK Cells." In Stem Cells and Regenerative Medicine. IOS Press, 2021. http://dx.doi.org/10.3233/bhr210028.

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Background: In peripheral blood, human natural killer (NK) cells are immunological cells that nearly don’t express the ectonucleotidase CD73 on their plasma membrane. When exposed to mesenchymal stem cells (MSCs), NK cells are able to acquire CD73. MSCs are known to be CD73-positive (CD73+) and also to modulate the immune system, e.g. through adenosynergic pathway by ectonucleosidases, such as CD73. Extracellular vesicles (EVs) are involved in cell-to-cell communication. Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) have emerged as paracrine mediators that are part of MSC immunomodulatory effects including immunosuppressive properties and immune privilege. Objective: The aim of our work was to study if CD73 could be acquired by NK cells through cell-to-cell communication with MSC-EVs as cell culture additives. We also hypothesised that MSC-EVs would act as tolerance inducers to attenuate NK cell cytotoxicity. Methods: Cell isolation was made from human umbilical cords for MSCs and from human peripheral blood for NK cells. MSC-EVs were isolated by ultracentrifugation and filtration, then characterized by nanoparticle tracking assay and flow cytometry (CD9, 63, 81 and 73). MSC-EV interaction with NK cells was monitored by PKH67 staining. NK cell activation was followed by measuring the expression of CD73 and NK-activating receptor natural-killer group 2, member D (NKG2D) by flow cytometry. The cytotoxicity of NK cells or EV-conditioned NK cells was evaluated after co-culture with K562 cells. Results: We showed that MSC-EVs are nanoparticles able to express CD73 and interact with NK cells. MSC-EV conditioned NK cells seem to increase CD73 and decrease NKG2D through an EV-mediated mechanism. MSC-EVs have an immunosuppressive effect on NK cells by preventing NK cell activation and NK cell cytotoxicity towards K562 cells. Conclusions: Our results demonstrate that MSC-EVs could influence NK cell behaviour and act as immunosuppressant cell-based products.
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Conference papers on the topic "Cell-Based cytotoxicity assay"

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Yum, Seungshic, Bong Gil Hyun, Kitae Rhie, and Kyoungsoon Shin. "ATP assay for rapid onboard testing to detect living microorganisms in Ballast Water." In IMarEST Ballast Water Technology Conference. IMarEST, 2017. http://dx.doi.org/10.24868/bwtc6.2017.012.

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Rapid and simple analytical methods for viable microorganism detection in ballast water are required to evaluate the efficiency of ballast water treatment system. During the course of systematic investigation of the cytotoxicity and apoptosis assays, it was found that the adenosine triphosphate (ATP) and luminescence based cell viability assay, in other word, an ATP assay was the most sensitive and applicable to ballast water management (BWM). The assay was applied to cultured microalgae samples, and it could detect the existence of 5 viable cells in 100 μl. Comparably low luminescent values w
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Znojek, Pawel, Sona Gurska, Petr Dzubak, and Marian Hajduch. "Abstract 3692: Development and validation of high content screening assay for identification of compounds based on cytotoxicity and cell cycle analysis using FUCCI probe." In Proceedings: AACR 106th Annual Meeting 2015; April 18-22, 2015; Philadelphia, PA. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1538-7445.am2015-3692.

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Al-Ansari, Dana E., Nura A. Mohamed, Isra Marei, Huseyin Yalcin, and Haissam Abou-Saleh. "Assessment of Metal Organic Framework as Potential Drug Carriers in Cardiovascular Diseases." In Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2020. http://dx.doi.org/10.29117/quarfe.2020.0127.

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Background: Cardiovascular diseases (CVDs) are considered the major cause of death worldwide. Therapeutic delivery to the cardiovascular system may play an important role in the successful treatment of a variety of CVDs, including atherosclerosis, ischemic-reperfusion injury, and microvascular diseases. Despite their clinical benefits, current therapeutic drugs are hindered by their short half-life and systemic side effects. This limitation could be overcome using controlled drug release with the potential for targeted drug delivery using a nanomedicine approach. In the current study, we have
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Chen, Kok Hao, and Jong Hyun Choi. "Nanoparticle-Aptamer: An Effective Growth Inhibitor for Human Cancer Cells." In ASME 2009 International Mechanical Engineering Congress and Exposition. ASMEDC, 2009. http://dx.doi.org/10.1115/imece2009-11966.

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Semiconductor nanocrystals have unique optical properties due to quantum confinement effects, and a variety of promising approaches have been devised to interface the nanomaterials with biomolecules for bioimaging and therapeutic applications. Such bio-interface can be facilitated via a DNA template for nanoparticles as oligonucleotides can mediate the aqueous-phase nucleation and capping of semiconductor nanocrystals.[1,2] Here, we report a novel scheme of synthesizing fluorescent nanocrystal quantum dots (NQDs) using DNA aptamers and the use of this biotic/abiotic nanoparticle system for gro
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Holt, Sanne, Sophie Vermond, Monique Hazenoot Hazenoot, Jeroen Overman, Jamil Aarbiou Aarbiou, and Jeroen DeGroot DeGroot. "Abstract 3232:In vitrocell based cytotoxicity and T cell activation assays to assess safety and efficacy of engineered T cell therapies." In Proceedings: AACR Annual Meeting 2020; April 27-28, 2020 and June 22-24, 2020; Philadelphia, PA. American Association for Cancer Research, 2020. http://dx.doi.org/10.1158/1538-7445.am2020-3232.

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Holt, Sanne L., Sophie Vermond, Monique Hazenoot, Rene McLaughlin, Marco Guadagnoli, and Marijn Vlaming. "Abstract 1373: Primary or iPSC-derived cell-based cytotoxicity assays to assess potential safety risks of engineered T cell therapiesin vitro." In Proceedings: AACR Annual Meeting 2021; April 10-15, 2021 and May 17-21, 2021; Philadelphia, PA. American Association for Cancer Research, 2021. http://dx.doi.org/10.1158/1538-7445.am2021-1373.

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