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Relevant bibliographies by topics / Cell-Cell Fusion Assays

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Dissertations / Theses
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Academic literature on the topic 'Cell-Cell Fusion Assays'

Author: Grafiati

Published: 4 June 2021

Last updated: 6 September 2023

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Journal articles on the topic "Cell-Cell Fusion Assays"

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Ji, Changhua, Jun Zhang, Nick Cammack, and Surya Sankuratri. "Development of a Novel Dual CCR5-Dependent and CXCR4-Dependent Cell-Cell Fusion Assay System with Inducible gp160 Expression." Journal of Biomolecular Screening 11, no. 1 (2005): 65–74. http://dx.doi.org/10.1177/1087057105282959.

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In the current study, a novel coreceptor-specific cell-cell fusion (CCF) assay system is reported. The system possesses the following features: dual CCR5-dependent and CXCR4-dependent CCF assays, all stable cell lines, inducible expression of gp160 to minimize cytotoxicity, robust luciferase reporter, and 384-well format. These assays have been validated using various known HIV entry inhibitors targeting various stages of the HIV entry/fusion process, including fusion inhibitors, gp120 inhibitors, CCR5 antagonists, CCR5 antibodies, and CXCR4 antagonists. IC 50data generated from this assay sys

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Smith, Elizabeth B., Robert A. Ogert, David Pechter, et al. "HIV Cell Fusion Assay." Journal of Biomolecular Screening 19, no. 1 (2013): 108–18. http://dx.doi.org/10.1177/1087057113500074.

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The health and disease-related biology of the CXCR4 chemokine receptor presents the challenge of finding a small molecule that can bind CXCR4 and block T-cell tropic human immunodeficiency virus type 1 (HIV-1) cell entry, while preserving the ability of CXCR4 to respond to its native ligand, CXCL12. HIV entry into the host cell involves the interaction of the viral envelope glycoprotein gp120 binding to CD4, followed by a rearrangement in gp120, and subsequent interaction with the chemokine receptor CXCR4 or CCR5. These initial events can be re-created in a cell fusion assay that represents a

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Kramer, Susanne, Peter Buontempo, Sony Agrawal, and Robert Ralston. "Imaging-Based Assay for Identification and Characterization of Inhibitors of CXCR4-Tropic HIV-1 Envelope-Dependent Cell-Cell Fusion." Journal of Biomolecular Screening 16, no. 6 (2011): 668–75. http://dx.doi.org/10.1177/1087057111403480.

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Infection of certain cell types by HIV results in formation of syncytia. This process can be blocked by antibodies or compounds that prevent interaction of viral envelope protein with host cell receptors. Here the authors describe an automated imaging-based assay for inhibitors of cell-cell fusion mediated by interaction of HIV gp120 with CXCR4 coreceptor. The assay quantifies syncytia formation between U87MG astrocytoma cells constitutively expressing CD4/CXCR4 and morphologically distinct Jurkat T lymphoma cells inducibly expressing HIV env. Each cell type was differentially labeled with vit

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Denning, G., and A. B. Fulton. "A simple trypsin resistance assay for muscle and other cell fusion." Journal of Histochemistry & Cytochemistry 34, no. 7 (1986): 959–62. http://dx.doi.org/10.1177/34.7.3086428.

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Muscle cells fusing in vitro have long provided biologists with a tool to study development and gene expression. However, many such studies used morphological assays of cell fusion. We present here a method for assaying fusion at a specific, operationally defined step. Muscle cells grown in monolayer are exposed to trypsin-EDTA solution at 37 degrees C; the trypsin is inactivated, the cells fixed in Lugol's iodine, and 200 to 300 nuclei are counted as being single or multiple. The presence of EDTA is important under standard conditions for muscle culture; however, little difference is seen in

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Cunyat, Francesc, Marta Curriu, Silvia Marfil, et al. "Evaluation of the Cytopathicity (Fusion/Hemifusion) of Patient-Derived HIV-1 Envelope Glycoproteins Comparing Two Effector Cell Lines." Journal of Biomolecular Screening 17, no. 6 (2012): 727–37. http://dx.doi.org/10.1177/1087057112439890.

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HIV-1 envelope glycoprotein (Env) is a major determinant of viral pathogenicity. The evaluation of the biological properties of patient-derived envelopes by comparing two effector cell lines (293T and HeLa) is reported. A standard cell-to-cell fusion assay was used to evaluate fusogenicity, whereas a coculture with CD4+ cells was used to evaluate absolute cell loss, single cell death, and hemifusion events. Fusion and absolute cell loss assays showed that Env-expressing 293T and HeLa cells had different fusion efficiencies; fusion was magnified in 293T cells despite a significantly lower cell-

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Zhou, Momei, Vivek Kamarshi, Ann M. Arvin, and Stefan L. Oliver. "Calcineurin phosphatase activity regulates Varicella-Zoster Virus induced cell-cell fusion." PLOS Pathogens 16, no. 11 (2020): e1009022. http://dx.doi.org/10.1371/journal.ppat.1009022.

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Cell-cell fusion (abbreviated as cell fusion) is a characteristic pathology of medically important viruses, including varicella-zoster virus (VZV), the causative agent of chickenpox and shingles. Cell fusion is mediated by a complex of VZV glycoproteins, gB and gH-gL, and must be tightly regulated to enable skin pathogenesis based on studies with gB and gH hyperfusogenic VZV mutants. Although the function of gB and gH-gL in the regulation of cell fusion has been explored, whether host factors are directly involved in this regulation process is unknown. Here, we discovered host factors that mod

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Kelly, James T., Stacey Human, Joseph Alderman, et al. "BST2/Tetherin Overexpression Modulates Morbillivirus Glycoprotein Production to Inhibit Cell–Cell Fusion." Viruses 11, no. 8 (2019): 692. http://dx.doi.org/10.3390/v11080692.

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The measles virus (MeV), a member of the genus Morbillivirus, is an established pathogen of humans. A key feature of morbilliviruses is their ability to spread by virus–cell and cell–cell fusion. The latter process, which leads to syncytia formation in vitro and in vivo, is driven by the viral fusion (F) and haemagglutinin (H) glycoproteins. In this study, we demonstrate that MeV glycoproteins are sensitive to inhibition by bone marrow stromal antigen 2 (BST2/Tetherin/CD317) proteins. BST2 overexpression causes a large reduction in MeV syncytia expansion. Using quantitative cell–cell fusion as

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Cairns, Tina M., Richard S. B. Milne, Manuel Ponce-de-Leon, Deanna K. Tobin, Gary H. Cohen, and Roselyn J. Eisenberg. "Structure-Function Analysis of Herpes Simplex Virus Type 1 gD and gH-gL: Clues from gDgH Chimeras." Journal of Virology 77, no. 12 (2003): 6731–42. http://dx.doi.org/10.1128/jvi.77.12.6731-6742.2003.

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ABSTRACT In alphaherpesviruses, glycoprotein B (gB), gD, gH, and gL are essential for virus entry. A replication-competent gL-null pseudorabies virus (PrV) (B. G. Klupp and T. C. Mettenleiter, J. Virol. 73:3014-3022, 1999) was shown to express a gDgH hybrid protein that could replace gD, gH, and gL in cell-cell fusion and null virus complementation assays. To study this phenomenon in herpes simplex virus type 1 (HSV-1), we constructed four gDgH chimeras, joining the first 308 gD amino acids to various gH N-terminal truncations. The chimeras were named for the first amino acid of gH at which ea

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Whitaker, Emily E., Nicholas J. Matheson, Sarah Perlee, Phillip B. Munson, Menelaos Symeonides, and Markus Thali. "EWI-2 Inhibits Cell–Cell Fusion at the HIV-1 Virological Presynapse." Viruses 11, no. 12 (2019): 1082. http://dx.doi.org/10.3390/v11121082.

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Cell-to-cell transfer of virus particles at the Env-dependent virological synapse (VS) is a highly efficient mode of HIV-1 transmission. While cell–cell fusion could be triggered at the VS, leading to the formation of syncytia and preventing exponential growth of the infected cell population, this is strongly inhibited by both viral (Gag) and host (ezrin and tetraspanins) proteins. Here, we identify EWI-2, a protein that was previously shown to associate with ezrin and tetraspanins, as a host factor that contributes to the inhibition of Env-mediated cell–cell fusion. Using quantitative fluores

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Broer, Rene, Bertrand Boson, Willy Spaan, François-Loïc Cosset, and Jeroen Corver. "Important Role for the Transmembrane Domain of Severe Acute Respiratory Syndrome Coronavirus Spike Protein during Entry." Journal of Virology 80, no. 3 (2006): 1302–10. http://dx.doi.org/10.1128/jvi.80.3.1302-1310.2006.

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ABSTRACT The spike protein (S) of severe acute respiratory syndrome coronavirus (SARS-CoV) is responsible for receptor binding and membrane fusion. It contains a highly conserved transmembrane domain that consists of three parts: an N-terminal tryptophan-rich domain, a central domain, and a cysteine-rich C-terminal domain. The cytoplasmic tail of S has previously been shown to be required for assembly. Here, the roles of the transmembrane and cytoplasmic domains of S in the infectivity and membrane fusion activity of SARS-CoV have been studied. SARS-CoV S-pseudotyped retrovirus (SARSpp) was us

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Dissertations / Theses on the topic "Cell-Cell Fusion Assays"

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Schirmacher, Anastasiya. "Modification of transmembrane peptides to probe SNARE-induced membrane fusion and cross-presentation of membrane-buried epitopes." Doctoral thesis, Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2020. http://hdl.handle.net/21.11130/00-1735-0000-0005-1576-F.

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Hurtley, S. M. "Reconstitution of an endocytic fusion event in a cell-free system." Thesis, Open University, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.484412.

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Mulampaka, Shiva Naresh. "Theoretical Studies of the Mechanisms of the Entry of Virus into Cells." Thesis, 2014. http://hdl.handle.net/2005/3082.

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Viruses cause human diseases by entering in to human cells. Many drugs have been developed that act at various stages of viral infection, but they fail due to their toxic side effects and high mutation rates of viruses. Recently, a new class of drugs called entry inhibitors has been developed which acts on the early stages of viral infection. These drugs have been developed by studying the entry process of viruses in to host cells. The success of these drugs, however, is still limited and research is being done to quantify the optimum dosage of these drugs and find new drugs targets. We develo

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Tsai, Yu-Chen, and 蔡佑晨. "Expression of the Envelope Glycoprotein and Establishment of a Cell Fusion Assay of Dengue Virus." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/95596026833282150742.

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碩士<br>國立臺灣大學<br>微生物學研究所<br>89<br>Dengue virus is a positive, single-stranded RNA enveloped flavivirus that is transmitted by mosquitoes, Aedes aegypti and Aedes albopictus. The virus is divided into four serotypes, DEN-1, DEN-2, DEN-3 and DEN-4. In the process of virus entry, the envelope protein, E protein, plays an important role. It is a glycoprotein of 495 amino acids, with a transmembrane domain at the C-terminal. It is believed to form a stable, non-covalently linked homodimer. The extracellular domains of E glycoprotein are thought to interact with the host cell receptor.

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Barszczewski, Marcin Miroslaw. "The establishment and characterization of an improved cell-free assay for exocytosis in neuroendocrine PC12 cells." Doctoral thesis, 2005. http://hdl.handle.net/11858/00-1735-0000-0006-AB9C-D.

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Lam, Vincent. "Development and Validation of a Novel Quantitative Assay for Cell surface Expression of GPCRs using a Receptor β-lactamase fusion Protein and the Colourometric Substrate Nitrocefin". Thesis, 2013. http://hdl.handle.net/1807/35620.

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Trafficking of GPCRs is a dynamic process that is tightly regulated and sometimes defective in human diseases. Therefore it is important to develop new methods to allow simple and quantitative measurement of surface expression of membrane proteins. Here we describe the development and validation of a new assay for quantification of cell surface expression of GPCRs using β-lactamase as a reporter. For this assay we N-terminally fused β-lactamase (βlac) to the β2-adrenergic receptor (β2AR) and GABA b R1 (GBR1). The results obtained by the βlac assay are quantitatively and qualitatively similar t

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Book chapters on the topic "Cell-Cell Fusion Assays"

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Shinn-Thomas, Jessica H., Victoria L. Scranton, and William A. Mohler. "Quantitative Assays for Cell Fusion." In Cell Fusion. Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-250-2_20.

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Grote, Eric. "Cell Fusion Assays for Yeast Mating Pairs." In Cell Fusion. Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-250-2_10.

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Drewlo, Sascha, Dora Baczyk, Caroline Dunk, and John Kingdom. "Fusion Assays and Models for the Trophoblast." In Cell Fusion. Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-250-2_21.

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Monreal, I. Abrrey, and Hector C. Aguilar. "Cell–Cell Fusion Assays to Study Henipavirus Entry and Evaluate Therapeutics." In Methods in Molecular Biology. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-3283-3_4.

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Lee, Jongkuen, and David Dominguez-Sola. "Mammalian Cell Fusion Assays for the Study of Cell Cycle Progression by Functional Complementation." In Cell Cycle Checkpoints. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1217-0_9.

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Bossart, Katharine N., and Christopher C. Broder. "Viral Glycoprotein-Mediated Cell Fusion Assays Using Vaccinia Virus Vectors." In Vaccinia Virus and Poxvirology. Humana Press, 2004. http://dx.doi.org/10.1385/1-59259-789-0:309.

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Inoue, Naokazu, and Masaru Okabe. "Sperm–Egg Fusion Assay in Mammals." In Cell Fusion. Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-250-2_19.

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York, Joanne, and Jack H. Nunberg. "A Cell-Cell Fusion Assay to Assess Arenavirus Envelope Glycoprotein Membrane-Fusion Activity." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-6981-4_10.

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Nakane, Shuhei, and Zene Matsuda. "Dual Split Protein (DSP) Assay to Monitor Cell–Cell Membrane Fusion." In Methods in Molecular Biology. Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2703-6_17.

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Dudzinski, Natasha R., Zhenyong Wu, and Erdem Karatekin. "A Nanodisc-Cell Fusion Assay with Single-Pore Sensitivity and Sub-millisecond Time Resolution." In Methods in Molecular Biology. Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-8760-3_17.

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Conference papers on the topic "Cell-Cell Fusion Assays"

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kariyone, S., H. kambayashi, T. satoh, T. uchida, H. ohto, and H. maeda. "A NEW MONOCLONAL ANTIBODY TO PLATELET MEMBRANE GLYCOPROTEINIV: EXPRESSION OF GLYCOPROTEIN IV ON PLATELETS, MEGAKRYOCYTES AND ERYTHROBLASTS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643531.

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A New monoclonal antibody, designated as TP85, was produced by fusion of the X63.Ag8.653 murine myeloma cell line with splenocytes from BALB/C mice immunized with washed human platelets. TP85 monoclonal antibody precipitated a single petide of 97,000 daltons in both reduced and nonreduced states by immune precipitation of 125I-labelled solubilized membrane. An isoelectric point was around pH 5.0. The antibody was IgG2b in isotype, as determined by the Ouchterlony immunodiffusion method.The reactivity of TP85 was examined by using indirect immuno-fluorescent assays and ABC immunoperoxidase meth

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Rathjen, Deborah A., and Carolyn L. Geczy. "PRODUCTION AND CHARACTERISATION OF A MONOCLONAL ANTIBODY AGAINST HUMAN ANTI-THROMBIN III." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644358.

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To study the role of anticoagulants, particularly antithrombin III (AT III) and heparin, on the activation of coagulation by monocytes/macrophages which have been stimulated with a soluble lymphocyte activation product, macrophage procoagulant inducing factor, we have prepared monoclonal antibodies (MAbs) to human AT III.In fusion experiments, in contrast to wells containing peritoneal feeder cells, positive hybrids were only found in wells containing medium conditioned by the macrophage cell line J774 (Rathjen and Geczy, 1986). Of 5 hybrids which initially produced antibody, only one hybrid,

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Jiang, Shibo, Lin Radigan, and Li Zhang. "Convenient cell fusion assay for rapid screening for HIV entry inhibitors." In BiOS 2000 The International Symposium on Biomedical Optics, edited by Patrick A. Limbach, John C. Owicki, Ramesh Raghavachari, and Weihong Tan. SPIE, 2000. http://dx.doi.org/10.1117/12.380514.

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Park, Taehyun, Timothy Jensen, Daniel Park, et al. "Capture of Very Rare Circulating Tumor Cells for Human Breast Cancer Diagnosis." In ASME 2007 International Mechanical Engineering Congress and Exposition. ASMEDC, 2007. http://dx.doi.org/10.1115/imece2007-42425.

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A method of collecting and delivering single or precise numbers of cells to assess the feasibility of capturing very rare circulating tumor cells for human breast cancer diagnosis and monitoring was developed. A PMMA device was assembled with minimal assembly variation using passive alignment. Thermoplastic fusion bonding was optimized to yield minimal deformation of the microfluidic channel. UV modification and an anti-epithelial cell adhesion molecule (anti-EpCAM) functionalization process were used to generate capture surfaces and maximized by control experiment. Single or precise numbers o

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Haber, Edgar, Marchall T. Runge, Christoph Bode, Betsy Branscomb, and Janet Schnee. "ANTIBODY TARGETED FIBRINOLYSIS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643723.

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Chemical conjugates of fibrin-specificantibodies and plasminogen activators. Urokinase or tPA were linked covalently toamonoclonal antibody specific for the amino terminus of the beta chain of human fibrin (59D8) by means of the unidirectionalcross-linking reagent SPDP. The fibrinolytic potency of the conjugates at equal amidolytic activities was compared to the native plasminogen activators in an assay measuring lysis of 1251-fibrin monomer covalently linked to Sepharose CL-4B. Urokinase was least potent, tPA exhibited a 10fold increase in fibrinolysis whereas both the urokinase and tPA antib

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Peterson, Sherket B., Zannatul Ferdous, Magnus Höök, and K. Jane Grande-Allen. "Decorin Deficient Cells Demonstrate Increased Proliferation and Altered Phenotypic Properties." In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-176043.

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Decorin (DCN), a class I member of the small leucine-rich proteoglycan (SLRP) family, is composed of a protein core of approximately 40kDa [1, 2] substituted with a single glycosaminoglycan (GAG) chain of chondroiton/dermatan sulfate on the N-terminal site [3]. DCN has been reported to interact with collagen [4,5] via its core protein, influence collagen fibrillogenesis [6], and inhibit the growth rates of various cell types when added exogenously to cell cultures [5,6]. There has recently been growing interest and studies in DCN related research using the knockout (KO) mice model which provid

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Cao, Ru, Kris Lea, Madhu Jasti, et al. "Abstract 5123: Characterization of genetic mutation spectra and identification of gene amplification and fusion variants in cell-free nucleic acid from cultured cancer cell media and liquid biopsy specimens using Oncomine™ Pan-Cancer Cell-Free Assay." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.sabcs18-5123.

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Cao, Ru, Kris Lea, Madhu Jasti, et al. "Abstract 5123: Characterization of genetic mutation spectra and identification of gene amplification and fusion variants in cell-free nucleic acid from cultured cancer cell media and liquid biopsy specimens using Oncomine™ Pan-Cancer Cell-Free Assay." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.am2019-5123.

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Heeke, Simon, Marius Ilié, Maryline Allegra, et al. "Abstract 5299: Detection of ALK fusion transcripts in plasma of non-small cell lung cancer patients using a novel RT-PCR based assay." In Proceedings: AACR Annual Meeting 2020; April 27-28, 2020 and June 22-24, 2020; Philadelphia, PA. American Association for Cancer Research, 2020. http://dx.doi.org/10.1158/1538-7445.am2020-5299.

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Jourdan, Adrian, Alexander Sarvadi, Hector R. Siller, and Huseyin Bostanci. "Additively Manufactured Liquid-Cooled Heat Sink: Gyroid-Based Design, Fabrication, and Testing." In ASME 2022 International Technical Conference and Exhibition on Packaging and Integration of Electronic and Photonic Microsystems. American Society of Mechanical Engineers, 2022. http://dx.doi.org/10.1115/ipack2022-97476.

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Abstract This study focuses on a topology-based design approach that can be applied to liquid-cooled heat sinks for high-heat-flux devices with the goal of improving heat dissipation and manufacturability. Specifically, the study investigates the use of additively manufactured topology-based lattice structures for the practicality and flexibility (of varying topology parameters) of complicated structures to achieve high heat transfer performance. The design restrictions were set such that the heat sink would occupy a 25 mm × 25 mm × 8 mm space and attach on a matching size heater. A gyroid lat

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Reports on the topic "Cell-Cell Fusion Assays"

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Fromm, A., Avihai Danon, and Jian-Kang Zhu. Genes Controlling Calcium-Enhanced Tolerance to Salinity in Plants. United States Department of Agriculture, 2003. http://dx.doi.org/10.32747/2003.7585201.bard.

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The specific objectives of the proposed research were to identify, clone and characterize downstream cellular target(s) of SOS3 in Arabidopsis thaliana, to analyze the Ca2+-binding characteristics of SOS3 and the sos3-1 mutant and their interactions with SOS3 cellular targets to analyze the SOS3 cell-specific expression patterns, and its subcellular localization, and to assess the in vivo role of SOS3 target protein(s) in plant tolerance to salinity stress. In the course of the study, in view of recent opportunities in identifying Ca2+ - responsive genes using microarrays, the group at Weizman

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Locy, Robert D., Hillel Fromm, Joe H. Cherry, and Narendra K. Singh. Regulation of Arabidopsis Glutamate Decarboxylase in Response to Heat Stress: Modulation of Enzyme Activity and Gene Expression. United States Department of Agriculture, 2001. http://dx.doi.org/10.32747/2001.7575288.bard.

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Most plants accumulate the nonprotein amino acid, g-aminobutyric acid (GABA), in response to heat stress. GABA is made from glutamate in a reaction catalyzed by glutamate decarboxylase (GAD), an enzyme that has been shown by the Israeli PI to be a calmodulin (CaM) binding protein whose activity is regulated in vitro by calcium and CaM. In Arabidopsis there are at least 5 GAD genes, two isoforms of GAD, GAD1 and GAD2, are known to be expressed, both of which appear to be calmodulin-binding proteins. The role of GABA accumulation in stress tolerance remains unclear, and thus the objectives of th

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