Log in

Relevant bibliographies by topics / Cell-Cell Fusion Assays / Journal articles

To see the other types of publications on this topic, follow the link: Cell-Cell Fusion Assays.

Journal articles on the topic 'Cell-Cell Fusion Assays'

Author: Grafiati

Published: 4 June 2021

Last updated: 6 September 2023

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Cell-Cell Fusion Assays.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Ji, Changhua, Jun Zhang, Nick Cammack, and Surya Sankuratri. "Development of a Novel Dual CCR5-Dependent and CXCR4-Dependent Cell-Cell Fusion Assay System with Inducible gp160 Expression." Journal of Biomolecular Screening 11, no. 1 (2005): 65–74. http://dx.doi.org/10.1177/1087057105282959.

Full text

Abstract:

In the current study, a novel coreceptor-specific cell-cell fusion (CCF) assay system is reported. The system possesses the following features: dual CCR5-dependent and CXCR4-dependent CCF assays, all stable cell lines, inducible expression of gp160 to minimize cytotoxicity, robust luciferase reporter, and 384-well format. These assays have been validated using various known HIV entry inhibitors targeting various stages of the HIV entry/fusion process, including fusion inhibitors, gp120 inhibitors, CCR5 antagonists, CCR5 antibodies, and CXCR4 antagonists. IC 50data generated from this assay sys

APA, Harvard, Vancouver, ISO, and other styles

2

Smith, Elizabeth B., Robert A. Ogert, David Pechter, et al. "HIV Cell Fusion Assay." Journal of Biomolecular Screening 19, no. 1 (2013): 108–18. http://dx.doi.org/10.1177/1087057113500074.

Full text

Abstract:

The health and disease-related biology of the CXCR4 chemokine receptor presents the challenge of finding a small molecule that can bind CXCR4 and block T-cell tropic human immunodeficiency virus type 1 (HIV-1) cell entry, while preserving the ability of CXCR4 to respond to its native ligand, CXCL12. HIV entry into the host cell involves the interaction of the viral envelope glycoprotein gp120 binding to CD4, followed by a rearrangement in gp120, and subsequent interaction with the chemokine receptor CXCR4 or CCR5. These initial events can be re-created in a cell fusion assay that represents a

APA, Harvard, Vancouver, ISO, and other styles

3

Kramer, Susanne, Peter Buontempo, Sony Agrawal, and Robert Ralston. "Imaging-Based Assay for Identification and Characterization of Inhibitors of CXCR4-Tropic HIV-1 Envelope-Dependent Cell-Cell Fusion." Journal of Biomolecular Screening 16, no. 6 (2011): 668–75. http://dx.doi.org/10.1177/1087057111403480.

Full text

Abstract:

Infection of certain cell types by HIV results in formation of syncytia. This process can be blocked by antibodies or compounds that prevent interaction of viral envelope protein with host cell receptors. Here the authors describe an automated imaging-based assay for inhibitors of cell-cell fusion mediated by interaction of HIV gp120 with CXCR4 coreceptor. The assay quantifies syncytia formation between U87MG astrocytoma cells constitutively expressing CD4/CXCR4 and morphologically distinct Jurkat T lymphoma cells inducibly expressing HIV env. Each cell type was differentially labeled with vit

APA, Harvard, Vancouver, ISO, and other styles

4

Denning, G., and A. B. Fulton. "A simple trypsin resistance assay for muscle and other cell fusion." Journal of Histochemistry & Cytochemistry 34, no. 7 (1986): 959–62. http://dx.doi.org/10.1177/34.7.3086428.

Full text

Abstract:

Muscle cells fusing in vitro have long provided biologists with a tool to study development and gene expression. However, many such studies used morphological assays of cell fusion. We present here a method for assaying fusion at a specific, operationally defined step. Muscle cells grown in monolayer are exposed to trypsin-EDTA solution at 37 degrees C; the trypsin is inactivated, the cells fixed in Lugol's iodine, and 200 to 300 nuclei are counted as being single or multiple. The presence of EDTA is important under standard conditions for muscle culture; however, little difference is seen in

APA, Harvard, Vancouver, ISO, and other styles

5

Cunyat, Francesc, Marta Curriu, Silvia Marfil, et al. "Evaluation of the Cytopathicity (Fusion/Hemifusion) of Patient-Derived HIV-1 Envelope Glycoproteins Comparing Two Effector Cell Lines." Journal of Biomolecular Screening 17, no. 6 (2012): 727–37. http://dx.doi.org/10.1177/1087057112439890.

Full text

Abstract:

HIV-1 envelope glycoprotein (Env) is a major determinant of viral pathogenicity. The evaluation of the biological properties of patient-derived envelopes by comparing two effector cell lines (293T and HeLa) is reported. A standard cell-to-cell fusion assay was used to evaluate fusogenicity, whereas a coculture with CD4+ cells was used to evaluate absolute cell loss, single cell death, and hemifusion events. Fusion and absolute cell loss assays showed that Env-expressing 293T and HeLa cells had different fusion efficiencies; fusion was magnified in 293T cells despite a significantly lower cell-

APA, Harvard, Vancouver, ISO, and other styles

6

Zhou, Momei, Vivek Kamarshi, Ann M. Arvin, and Stefan L. Oliver. "Calcineurin phosphatase activity regulates Varicella-Zoster Virus induced cell-cell fusion." PLOS Pathogens 16, no. 11 (2020): e1009022. http://dx.doi.org/10.1371/journal.ppat.1009022.

Full text

Abstract:

Cell-cell fusion (abbreviated as cell fusion) is a characteristic pathology of medically important viruses, including varicella-zoster virus (VZV), the causative agent of chickenpox and shingles. Cell fusion is mediated by a complex of VZV glycoproteins, gB and gH-gL, and must be tightly regulated to enable skin pathogenesis based on studies with gB and gH hyperfusogenic VZV mutants. Although the function of gB and gH-gL in the regulation of cell fusion has been explored, whether host factors are directly involved in this regulation process is unknown. Here, we discovered host factors that mod

APA, Harvard, Vancouver, ISO, and other styles

7

Kelly, James T., Stacey Human, Joseph Alderman, et al. "BST2/Tetherin Overexpression Modulates Morbillivirus Glycoprotein Production to Inhibit Cell–Cell Fusion." Viruses 11, no. 8 (2019): 692. http://dx.doi.org/10.3390/v11080692.

Full text

Abstract:

The measles virus (MeV), a member of the genus Morbillivirus, is an established pathogen of humans. A key feature of morbilliviruses is their ability to spread by virus–cell and cell–cell fusion. The latter process, which leads to syncytia formation in vitro and in vivo, is driven by the viral fusion (F) and haemagglutinin (H) glycoproteins. In this study, we demonstrate that MeV glycoproteins are sensitive to inhibition by bone marrow stromal antigen 2 (BST2/Tetherin/CD317) proteins. BST2 overexpression causes a large reduction in MeV syncytia expansion. Using quantitative cell–cell fusion as

APA, Harvard, Vancouver, ISO, and other styles

8

Cairns, Tina M., Richard S. B. Milne, Manuel Ponce-de-Leon, Deanna K. Tobin, Gary H. Cohen, and Roselyn J. Eisenberg. "Structure-Function Analysis of Herpes Simplex Virus Type 1 gD and gH-gL: Clues from gDgH Chimeras." Journal of Virology 77, no. 12 (2003): 6731–42. http://dx.doi.org/10.1128/jvi.77.12.6731-6742.2003.

Full text

Abstract:

ABSTRACT In alphaherpesviruses, glycoprotein B (gB), gD, gH, and gL are essential for virus entry. A replication-competent gL-null pseudorabies virus (PrV) (B. G. Klupp and T. C. Mettenleiter, J. Virol. 73:3014-3022, 1999) was shown to express a gDgH hybrid protein that could replace gD, gH, and gL in cell-cell fusion and null virus complementation assays. To study this phenomenon in herpes simplex virus type 1 (HSV-1), we constructed four gDgH chimeras, joining the first 308 gD amino acids to various gH N-terminal truncations. The chimeras were named for the first amino acid of gH at which ea

APA, Harvard, Vancouver, ISO, and other styles

9

Whitaker, Emily E., Nicholas J. Matheson, Sarah Perlee, Phillip B. Munson, Menelaos Symeonides, and Markus Thali. "EWI-2 Inhibits Cell–Cell Fusion at the HIV-1 Virological Presynapse." Viruses 11, no. 12 (2019): 1082. http://dx.doi.org/10.3390/v11121082.

Full text

Abstract:

Cell-to-cell transfer of virus particles at the Env-dependent virological synapse (VS) is a highly efficient mode of HIV-1 transmission. While cell–cell fusion could be triggered at the VS, leading to the formation of syncytia and preventing exponential growth of the infected cell population, this is strongly inhibited by both viral (Gag) and host (ezrin and tetraspanins) proteins. Here, we identify EWI-2, a protein that was previously shown to associate with ezrin and tetraspanins, as a host factor that contributes to the inhibition of Env-mediated cell–cell fusion. Using quantitative fluores

APA, Harvard, Vancouver, ISO, and other styles

10

Broer, Rene, Bertrand Boson, Willy Spaan, François-Loïc Cosset, and Jeroen Corver. "Important Role for the Transmembrane Domain of Severe Acute Respiratory Syndrome Coronavirus Spike Protein during Entry." Journal of Virology 80, no. 3 (2006): 1302–10. http://dx.doi.org/10.1128/jvi.80.3.1302-1310.2006.

Full text

Abstract:

ABSTRACT The spike protein (S) of severe acute respiratory syndrome coronavirus (SARS-CoV) is responsible for receptor binding and membrane fusion. It contains a highly conserved transmembrane domain that consists of three parts: an N-terminal tryptophan-rich domain, a central domain, and a cysteine-rich C-terminal domain. The cytoplasmic tail of S has previously been shown to be required for assembly. Here, the roles of the transmembrane and cytoplasmic domains of S in the infectivity and membrane fusion activity of SARS-CoV have been studied. SARS-CoV S-pseudotyped retrovirus (SARSpp) was us

APA, Harvard, Vancouver, ISO, and other styles

11

García-Murria, María, Neus Expósito-Domínguez, Gerard Duart, Ismael Mingarro, and Luis Martinez-Gil. "A Bimolecular Multicellular Complementation System for the Detection of Syncytium Formation: A New Methodology for the Identification of Nipah Virus Entry Inhibitors." Viruses 11, no. 3 (2019): 229. http://dx.doi.org/10.3390/v11030229.

Full text

Abstract:

Fusion of viral and cellular membranes is a key step during the viral life cycle. Enveloped viruses trigger this process by means of specialized viral proteins expressed on their surface, the so-called viral fusion proteins. There are multiple assays to analyze the viral entry including those that focus on the cell-cell fusion induced by some viral proteins. These methods often rely on the identification of multinucleated cells (syncytium) as a result of cell membrane fusions. In this manuscript, we describe a novel methodology for the study of cell-cell fusion. Our approach, named Bimolecular

APA, Harvard, Vancouver, ISO, and other styles

12

Xia, Zhenbiao, Relja Popovic, Tara Lorenz, Donna Santillan, Frank Erfurth, and Nancy J. Zeleznik-Le. "MLL-AF4 Down-Regulates CDKN1B (P27) Independent of Cell Cycle Progression." Blood 104, no. 11 (2004): 2563. http://dx.doi.org/10.1182/blood.v104.11.2563.2563.

Full text

Abstract:

Abstract The MLL gene, involved in many chromosomal translocations associated with acute myeloid and lymphoid leukemia, has more than forty known partner genes with which it is able to form in- frame fusions. MLL fusion genes transform hematopoietic cells in vitro, and cause leukemia in mouse models. However, the mechanism is still not clear. Characterizing important downstream target genes may provide rational therapeutic strategies for the treatment of MLL-associated leukemia. We explored potential downstream target genes of the most prevalent MLL fusion protein, MLL-AF4, which is primarily

APA, Harvard, Vancouver, ISO, and other styles

13

Leippe, Donna M., Kate Qin Zhao, Kevin Hsiao, and Michael R. Slater. "Cell-Free Expression of Protein Kinase a for Rapid Activity Assays." Analytical Chemistry Insights 5 (January 2010): ACI.S4732. http://dx.doi.org/10.4137/aci.s4732.

Full text

Abstract:

Functional protein analysis often calls for lengthy, laborious in vivo protein expression and purification, and can be complicated by the lack of stability of the purified protein. In this study, we demonstrate the feasibility of a simplified procedure for functional protein analysis on magnetic particles using cell-free protein synthesis of the catalytic subunit of human cAMP-dependent protein kinase as a HaloTag® fusion protein. The cell-free protein synthesis systems provide quick access to the protein of interest, while the HaloTag technology provides efficient, covalent protein immobiliza

APA, Harvard, Vancouver, ISO, and other styles

14

Bradley, Joe, Jasween Gill, Francois Bertelli, et al. "Development and Automation of a 384-Well Cell Fusion Assay to Identify Inhibitors of CCR5/CD4-Mediated HIV Virus Entry." Journal of Biomolecular Screening 9, no. 6 (2004): 516–24. http://dx.doi.org/10.1177/1087057104264577.

Full text

Abstract:

This article describes the automation of an in vitro cell-based fusion assay for the identification of novel inhibitors of receptor mediated HIV-1 entry. The assay utilises two stable cell lines: one expressing CD4, CCR5 and an LTR-promoter/β-galactosidase reporter construct, and the other expressing gp160 and tat. Accumulation of β-galactosidase can only occur following fusion of these two cell lines via the gp160 and receptor mediators, as this event facilitates the transfer of the tat transcription factor between the two cell types. Although similar cell fusion systems have been described p

APA, Harvard, Vancouver, ISO, and other styles

15

Zhao, Min, Pei-Yi Su, Danielle A. Castro, et al. "Rapid, reliable, and reproducible cell fusion assay to quantify SARS-Cov-2 spike interaction with hACE2." PLOS Pathogens 17, no. 6 (2021): e1009683. http://dx.doi.org/10.1371/journal.ppat.1009683.

Full text

Abstract:

COVID-19 is a global crisis of unimagined dimensions. Currently, Remedesivir is only fully licensed FDA therapeutic. A major target of the vaccine effort is the SARS-CoV-2 spike-hACE2 interaction, and assessment of efficacy relies on time consuming neutralization assay. Here, we developed a cell fusion assay based upon spike-hACE2 interaction. The system was tested by transient co-transfection of 293T cells, which demonstrated good correlation with standard spike pseudotyping for inhibition by sera and biologics. Then established stable cell lines were very well behaved and gave even better co

APA, Harvard, Vancouver, ISO, and other styles

16

Singethan, K., E. Topfstedt, S. Schubert, W. P. Duprex, B. K. Rima, and Jürgen Schneider-Schaulies. "CD9-dependent regulation of Canine distemper virus-induced cell–cell fusion segregates with the extracellular domain of the haemagglutinin." Journal of General Virology 87, no. 6 (2006): 1635–42. http://dx.doi.org/10.1099/vir.0.81629-0.

Full text

Abstract:

Antibodies to CD9, a member of the tetraspan transmembrane-protein family, selectively inhibit Canine distemper virus (CDV)-induced cell–cell fusion. Neither CDV-induced virus–cell fusion nor cell–cell fusion induced by the closely related morbillivirus Measles virus (MV) is affected by anti-CD9 antibodies. As CDV does not bind CD9, an unknown, indirect mechanism is responsible for the observed inhibition of cell–cell fusion. It was investigated whether this effect was restricted to only one viral glycoprotein, either the haemagglutinin (H) or the fusion (F) protein, which form a fusion comple

APA, Harvard, Vancouver, ISO, and other styles

17

Ramesh, Archana, Samuel Koo, Soo Jin Kang, et al. "A Novel NGS-Based Simultaneous Detection of DNA and RNA Biomarkers Using Total Nucleic Acid (TNA) for Acute Lymphocytic Leukemia (ALL)." Blood 138, Supplement 1 (2021): 1875. http://dx.doi.org/10.1182/blood-2021-148012.

Full text

Abstract:

Abstract Background: Acute Lymphocytic Leukemia (ALL) is the most common childhood cancer and accounts for about a quarter of adult acute leukemias. Current NCCN recommendations for clinical testing for risk stratification and treatment guidance include karyotyping, FISH testing for translocations, and RT-PCR for gene fusions and sequencing for DNA mutations detection. Most NGS based approaches test DNA mutations and RNA fusions separately, thereby requiring higher input material and multiple workflows adding to the cost and turn-around-time. An NGS based assay for the detection of DNA variant

APA, Harvard, Vancouver, ISO, and other styles

18

Branscomb, Elizabeth E., Marschall S. Runge, Christopher E. Savard, Keith M. Adams, Gary R. Matsueda, and Edgar Haber. "Bispecific Monoclonal Antibodies Produced by Somatic Cell Fusion Increase the Potency of Tissue Plasminogen Activator." Thrombosis and Haemostasis 64, no. 02 (1990): 260–66. http://dx.doi.org/10.1055/s-0038-1647297.

Full text

Abstract:

SummaryBispecific monoclonal antibodies that bind simultaneously to human fibrin and tissue plasminogen activator (tPA) enhance the fibrinolytic potency of tPA. Two bispecific antibodies (F36.23 and F32.1) were generated by somatic cell fusion. Antibody F36.23 derives its tPA binding from monoclonal anti-tPA antibody TCL8 and its fibrin binding from monoclonal antifibrin antibody 59D8. After purification from cell supernatants and ascites by two steps of affinity chromatography, hybrid-hybridoma bispecific antibody F36.23 simultaneously bound tPA and fibrin in solution and in solid-phase assay

APA, Harvard, Vancouver, ISO, and other styles

19

Li, Zhuo, Cher Hung, Reay G. Paterson, et al. "Type II integral membrane protein, TM of J paramyxovirus promotes cell-to-cell fusion." Proceedings of the National Academy of Sciences 112, no. 40 (2015): 12504–9. http://dx.doi.org/10.1073/pnas.1509476112.

Full text

Abstract:

Paramyxoviruses include many important animal and human pathogens. Most paramyxoviruses have two integral membrane proteins: fusion protein (F) and attachment proteins hemagglutinin, hemagglutinin–neuraminidase, or glycoprotein (G), which are critical for viral entry into cells. J paramyxovirus (JPV) encodes four integral membrane proteins: F, G, SH, and transmembrane (TM). The function of TM is not known. In this work, we have generated a viable JPV lacking TM (JPV∆TM). JPV∆TM formed opaque plaques compared with JPV. Quantitative syncytia assays showed that JPV∆TM was defective in promoting c

APA, Harvard, Vancouver, ISO, and other styles

20

Pang, Wei, Rui-Rui Wang, Yue-Dong Gao, et al. "A Novel Enzyme-Linked Immunosorbent Assay for Screening HIV-1 Fusion Inhibitors Targeting HIV-1 Gp41 Core Structure." Journal of Biomolecular Screening 16, no. 2 (2011): 221–29. http://dx.doi.org/10.1177/1087057110393333.

Full text

Abstract:

The gp41 subunit of the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein mediates the fusion of viral and host cell membranes. As the HIV-1 enters the host cells, the 2 helical regions, HR1 and HR2, in the ectodomain of gp41 can form a 6-helix bundle, which brings the viral and target cell membranes to close proximity and serves as an attractive target for developing HIV-1 fusion inhibitors. Now, there are several cell- and molecule-based assays to identify potential HIV-1 fusion inhibitors targeting gp41. However, these assays cannot be used universally because they are time-

APA, Harvard, Vancouver, ISO, and other styles

21

Denis, Marc G., Audrey Vallee, Christine Sagan, et al. "Detection of ALK and ROS1 fusion transcripts in FFPE samples of non-small cell lung cancer patients using a novel RT-PCR based assay and targeted RNA sequencing." Journal of Clinical Oncology 38, no. 15_suppl (2020): e21695-e21695. http://dx.doi.org/10.1200/jco.2020.38.15_suppl.e21695.

Full text

Abstract:

e21695 Background: Detection of genomic rearrangements like ALK and ROS1 fusions are mandatory in non-small cell lung cancer (NSCLC) as those alterations can be targeted by an increasing number of drugs. Fluorescence in-situ hybridization (FISH) has been so far the gold standard but multiplexed technologies would allow analysis of many potential targets simultaneously. We have evaluated a novel RT-PCR based assay and a targeted RNA sequencing solution for the detection of ALK and ROS1 rearrangement in NSCLC patients. Methods: 41 patients with late stage NSCLC were included in the study. ALK an

APA, Harvard, Vancouver, ISO, and other styles

22

Zimmerberg, J., R. Blumenthal, D. P. Sarkar, M. Curran, and S. J. Morris. "Restricted movement of lipid and aqueous dyes through pores formed by influenza hemagglutinin during cell fusion." Journal of Cell Biology 127, no. 6 (1994): 1885–94. http://dx.doi.org/10.1083/jcb.127.6.1885.

Full text

Abstract:

The fusion of cells by influenza hemagglutinin (HA) is the best characterized example of protein-mediated membrane fusion. In simultaneous measurements of pairs of assays for fusion, we determined the order of detectable events during fusion. Fusion pore formation in HA-triggered cell-cell fusion was first detected by changes in cell membrane capacitance, next by a flux of fluorescent lipid, and finally by flux of aqueous fluorescent dye. Fusion pore conductance increased by small steps. A retardation of lipid and aqueous dyes occurred during fusion pore fluctuations. The flux of aqueous dye d

APA, Harvard, Vancouver, ISO, and other styles

23

Mirzayans, Razmik, and David Murray. "Intratumor Heterogeneity and Therapy Resistance: Contributions of Dormancy, Apoptosis Reversal (Anastasis) and Cell Fusion to Disease Recurrence." International Journal of Molecular Sciences 21, no. 4 (2020): 1308. http://dx.doi.org/10.3390/ijms21041308.

Full text

Abstract:

A major challenge in treating cancer is posed by intratumor heterogeneity, with different sub-populations of cancer cells within the same tumor exhibiting therapy resistance through different biological processes. These include therapy-induced dormancy (durable proliferation arrest through, e.g., polyploidy, multinucleation, or senescence), apoptosis reversal (anastasis), and cell fusion. Unfortunately, such responses are often overlooked or misinterpreted as “death” in commonly used preclinical assays, including the in vitro colony-forming assay and multiwell plate “viability” or “cytotoxicit

APA, Harvard, Vancouver, ISO, and other styles

24

Zheng, Li-Mou, David B. Whyte, Li Ruan, et al. "Screening of NSCLC samples from Chinese lung cancer patients for activating rearrangements of the ALK, RET, and ROS1 genes." Journal of Clinical Oncology 31, no. 15_suppl (2013): e22066-e22066. http://dx.doi.org/10.1200/jco.2013.31.15_suppl.e22066.

Full text

Abstract:

e22066 Background: The ALK, RET, and ROS1 genes are involved in gene rearrangements in a fraction of non-small cell lung cancers. The resulting oncogenic fusion genes define molecular sub-types of NSCLC with distinct sensitivities to treatment with various kinase inhibitors. We developed real-time reverse transcriptase PCR assays to detect rearrangements of ALK, RET, and ROS1 in FFPE lung cancer tissue. Methods: mRNA from NSCLC FFPE tissue samples was reverse transcribed to cDNA. Multiplex quantitative PCR was performed to detect 9 variants of EML4-ALK fusions, 9 variants of RET fusions and 14

APA, Harvard, Vancouver, ISO, and other styles

25

Harte, James V., Samantha L. Wakerlin, Andrew J. Lindsay, Justin V. McCarthy, and Caroline Coleman-Vaughan. "Metalloprotease-Dependent S2′-Activation Promotes Cell–Cell Fusion and Syncytiation of SARS-CoV-2." Viruses 14, no. 10 (2022): 2094. http://dx.doi.org/10.3390/v14102094.

Full text

Abstract:

SARS-CoV-2 cell–cell fusion and syncytiation is an emerging pathomechanism in COVID-19, but the precise factors contributing to the process remain ill-defined. In this study, we show that metalloproteases promote SARS-CoV-2 spike protein-induced syncytiation in the absence of established serine proteases using in vitro cell–cell fusion assays. We also show that metalloproteases promote S2′-activation of the SARS-CoV-2 spike protein, and that metalloprotease inhibition significantly reduces the syncytiation of SARS-CoV-2 variants of concern. In the presence of serine proteases, however, metallo

APA, Harvard, Vancouver, ISO, and other styles

26

Liu, Fengling, Gaby Marquardt, Austin N. Kirschner, Richard Longnecker, and Theodore S. Jardetzky. "Mapping the N-Terminal Residues of Epstein-Barr Virus gp42 That Bind gH/gL by Using Fluorescence Polarization and Cell-Based Fusion Assays." Journal of Virology 84, no. 19 (2010): 10375–85. http://dx.doi.org/10.1128/jvi.00381-10.

Full text

Abstract:

ABSTRACT Epstein-Barr virus (EBV) requires at a minimum membrane-associated glycoproteins gB, gH, and gL for entry into host cells. B-cell entry additionally requires gp42, which binds to gH/gL and triggers viral entry into B cells. The presence of soluble gp42 inhibits membrane fusion with epithelial cells by forming a stable heterotrimer of gH/gL/gp42. The interaction of gp42 with gH/gL has been previously mapped to residues 36 to 81 at the N-terminal region of gp42. In this study, we further mapped this region to identify essential features for binding to gH/gL by use of synthetic peptides.

APA, Harvard, Vancouver, ISO, and other styles

27

Pasieka, Tracy Jo, Lucie Maresova, Kimiyasu Shiraki, and Charles Grose. "Regulation of Varicella-Zoster Virus-Induced Cell-to-Cell Fusion by the Endocytosis-Competent Glycoproteins gH and gE." Journal of Virology 78, no. 6 (2004): 2884–96. http://dx.doi.org/10.1128/jvi.78.6.2884-2896.2004.

Full text

Abstract:

ABSTRACT The gH glycoprotein of varicella-zoster virus (VZV) is a major fusogen. The realigned short cytoplasmic tail of gH (18 amino acids) harbors a functional endocytosis motif (YNKI) that mediates internalization in both VZV-infected and transfected cells (T. J. Pasieka, L. Maresova, and C. Grose, J. Virol. 77: 4194-4202, 2003). During subsequent confocal microscopy studies of endocytosis-deficient gH mutants, we observed that cells transfected with the gH tail mutants exhibited marked fusion. Therefore, we postulated that VZV gH endocytosis served to regulate cell-to-cell fusion. Subseque

APA, Harvard, Vancouver, ISO, and other styles

28

Lackman-Smith, Carol, Clay Osterling, Katherine Luckenbaugh, et al. "Development of a Comprehensive Human Immunodeficiency Virus Type 1 Screening Algorithm for Discovery and Preclinical Testing of Topical Microbicides." Antimicrobial Agents and Chemotherapy 52, no. 5 (2008): 1768–81. http://dx.doi.org/10.1128/aac.01328-07.

Full text

Abstract:

ABSTRACT Topical microbicides are self-administered, prophylactic products for protection against sexually transmitted pathogens. A large number of compounds with known anti-human immunodeficiency virus type 1 (HIV-1) inhibitory activity have been proposed as candidate topical microbicides. To identify potential leads, an in vitro screening algorithm was developed to evaluate candidate microbicides in assays that assess inhibition of cell-associated and cell-free HIV-1 transmission, entry, and fusion. The algorithm advances compounds by evaluation in a series of defined assays that generate me

APA, Harvard, Vancouver, ISO, and other styles

29

Krishnan, Anuja, Santosh K. Verma, Prashant Mani, Rahul Gupta, Suman Kundu, and Debi P. Sarkar. "A Histidine Switch in Hemagglutinin-Neuraminidase Triggers Paramyxovirus-Cell Membrane Fusion." Journal of Virology 83, no. 4 (2008): 1727–41. http://dx.doi.org/10.1128/jvi.02026-08.

Full text

Abstract:

ABSTRACT Most paramyxovirus fusion proteins require coexpression of and activation by a homotypic attachment protein, hemagglutinin-neuraminidase (HN), to promote membrane fusion. However, the molecular mechanism of the activation remains unknown. We previously showed that the incorporation of a monohistidylated lipid into F-virosome (Sendai viral envelope containing only fusion protein) enhanced its fusion to hepatocytes, suggesting that the histidine residue in the lipid accelerated membrane fusion. Therefore, we explored whether a histidine moiety in HN could similarly direct activation of

APA, Harvard, Vancouver, ISO, and other styles

30

Jones, A. T., D. J. Spiro, T. Kirchhausen, P. Melancon, and M. Wessling-Resnick. "Studies on the inhibition of endosome fusion by GTPgammaS-bound ARF." Journal of Cell Science 112, no. 20 (1999): 3477–85. http://dx.doi.org/10.1242/jcs.112.20.3477.

Full text

Abstract:

Using a cell free assay, we have previously shown that ARF is not required for endosome fusion but that inhibition of fusion by GTPgammaS is dependent on a cytosolic pool of ARFs. Since ARF is proposed to function in intracellular membrane traffic by promoting vesicle biogenesis, and components of clathrin- and COP-coated vesicles have been localized on endosomal structures, we investigated whether ARF-mediated inhibition of early endosome fusion involves the recruitment or irreversible association of these proteins onto endosomal membranes. We now report that depletion of components of clathr

APA, Harvard, Vancouver, ISO, and other styles

31

Jones, Thomas R., Shi-Wu Lee, Stephen V. Johann, et al. "Specific Inhibition of Human Cytomegalovirus Glycoprotein B-Mediated Fusion by a Novel Thiourea Small Molecule." Journal of Virology 78, no. 3 (2004): 1289–300. http://dx.doi.org/10.1128/jvi.78.3.1289-1300.2004.

Full text

Abstract:

ABSTRACT A novel small molecule inhibitor of human cytomegalovirus (HCMV) was identified as the result of screening a chemical library by using a whole-virus infected-cell assay. Synthetic chemistry efforts yielded the analog designated CFI02, a compound whose potency had been increased about 100-fold over an initial inhibitor. The inhibitory concentration of CFI02 in various assays is in the low nanomolar range. CFI02 is a selective and potent inhibitor of HCMV; it has no activity against other CMVs, alphaherpesviruses, or unrelated viruses. Mechanism-of-action studies indicate that CFI02 act

APA, Harvard, Vancouver, ISO, and other styles

32

Skinner, Amy M., Devorah C. Goldman, Andrea McBeth, Matthew J. Shurtleff, Harv W. Fleming, and Peter Kurre. "Hematopoietic Cells Undergo Homotypic Cell Fusion: A Novel Platform to Study Genomic Instability and Leukemogenesis." Blood 116, no. 21 (2010): 1196. http://dx.doi.org/10.1182/blood.v116.21.1196.1196.

Full text

Abstract:

Abstract Abstract 1196 Gene copy number variation (CNV) and translocation-derived gene fusion products are commonly observed in hematological malignancies. Mechanisms precipitating CNV include failed cytokinesis or spontaneous cell fusion. Heterotypic fusion of hematopoietic cells with non-hematopoeitic cells has been reported following injury in diverse tissues. Cell fusion involves blending membranes, intracellular material, and nuclear material from two parental cells to form a genetically and immunophenotypically distinct (often hyperdiploid) single daughter cell. We therefore hypothesized

APA, Harvard, Vancouver, ISO, and other styles

33

Romer, Daniela, David W. Brighty, Cynthia L. Robson, and Quentin J. Sattentau. "Candidate Polyanionic Microbicides Inhibit Human T-Cell Lymphotropic Virus Type 1 Receptor Interactions, Cell-Free Infection, and Cell-Cell Spread." Antimicrobial Agents and Chemotherapy 53, no. 2 (2008): 678–87. http://dx.doi.org/10.1128/aac.01550-07.

Full text

Abstract:

ABSTRACT The human T-cell lymphotropic virus type 1 (HTLV-1) is the cause of adult T-cell leukemia and inflammatory diseases including HTLV-1-associated myelopathy/tropical spastic paraparesis. HTLV-1 can be transmitted through sexual contact, mother-to-child transmission, and exposure to contaminated blood. Microbicides are agents that interfere with microbial infectivity at mucous membranes, and candidates are under development for use against sexually transmitted viruses such as human immunodeficiency virus type 1. We previously demonstrated that cell surface polyanionic heparan sulfate pro

APA, Harvard, Vancouver, ISO, and other styles

34

Cygan, Erin E., Ravi K. Shah, Tanya Kozlik, Paul G. Thomas, and Anthony E. Zamora. "Abstract 2992: Conserved gene fusion ETV6/RUNX1 displays higher immunogenicity than patient-specific missense mutations within the context of HLA-A*02:01." Cancer Research 83, no. 7_Supplement (2023): 2992. http://dx.doi.org/10.1158/1538-7445.am2023-2992.

Full text

Abstract:

Abstract Tumor-specific antigens can be targeted by T cells and cancer immunotherapies directed against these neoantigens have resulted in regression across several cancer types. Both missense mutations and gene fusions can serve as targets for cytotoxic T cells, but it is unknown whether one of these classes of mutations has unique parameters that make it more immunogenic. This study compares the immunogenicity of patient-specific missense mutations and a conserved gene fusion, ETV6/RUNX1, present in approximately 25% of pediatric B cell acute lymphoblastic leukemia cases to determine which i

APA, Harvard, Vancouver, ISO, and other styles

35

Zhong, Elizabeth H., Carola Ledderose, Paola De Andrade Mello, et al. "Structural and functional characterization of engineered bifunctional fusion proteins of CD39 and CD73 ectonucleotidases." American Journal of Physiology-Cell Physiology 320, no. 1 (2021): C15—C29. http://dx.doi.org/10.1152/ajpcell.00430.2020.

Full text

Abstract:

Extracellular diphosphate and triphosphate nucleotides are released from activated or injured cells to trigger vascular and immune P2 purinergic receptors, provoking inflammation and vascular thrombosis. These metabokines are scavenged by ectonucleoside triphosphate diphosphohydrolase-1 (E-NTPDase1 or CD39). Further degradation of the monophosphate nucleoside end products occurs by surface ecto-5′-nucleotidase (NMPase) or CD73. These ectoenzymatic processes work in tandem to promote adenosinergic responses, which are immunosuppressive and antithrombotic. These homeostatic ectoenzymatic mechani

APA, Harvard, Vancouver, ISO, and other styles

36

Bories, Phuong-Nhi, Patrick Younes, Marc Zerbib, et al. "TMPRSS2-ERG Fusion Transcripts in Matched Urine and Needle Rinse Material after Biopsy for the Detection of Prostate Cancer." Clinical Chemistry 59, no. 1 (2013): 245–51. http://dx.doi.org/10.1373/clinchem.2012.192260.

Full text

Abstract:

BACKGROUND Current methods for detecting TMPRSS2-ERG fusion transcript in the urine of patients with suspected prostate cancer lack diagnostic sensitivity. We combined urine and prostate biopsy rinse material (BRM) assays to improve the fusion gene detection rate. METHODS Eighty patients with clinical and/or prostate-specific antigen suspicion of prostate cancer were prospectively included in the study. Urine samples were collected before and after prostate biopsy, and BRM was collected from the biopsy needle. We used reverse-transcription PCR (RT-PCR) for the detection of fusion transcripts.

APA, Harvard, Vancouver, ISO, and other styles

37

Haferlach, Claudia, Niroshan Nadarajah, Manja Meggendorfer, et al. "Targeted RNA Sequencing Is Capable of Identifying Thus Far Unknown Partner Genes in Leukemias with Rare Translocations and Provides Important Clinical and Therapeutical Information." Blood 128, no. 22 (2016): 2857. http://dx.doi.org/10.1182/blood.v128.22.2857.2857.

Full text

Abstract:

Abstract Background: The genomic landscape of hematological malignancies has been resolved mainly based on whole exome and whole genome sequencing, primarily targeting gene mutations. Beside mutations also gene fusions function as therapeutic targets, impressively shown for e.g. BCR-ABL1 and ETV6-PDGFRB. Hence, the need for a comprehensive genetic analysis is increasing, as it is the basis for precision medicine, selecting treatment based on genotype and providing markers for disease monitoring. Aim: To test the value of targeted RNA sequencing in a routine diagnostic work up. Patients and Met

APA, Harvard, Vancouver, ISO, and other styles

38

Carlson, Coby B., Matthew B. Robers, Kurt W. Vogel, and Thomas Machleidt. "Development of LanthaScreen™ Cellular Assays for Key Components within the PI3K/AKT/mTOR Pathway." Journal of Biomolecular Screening 14, no. 2 (2009): 121–32. http://dx.doi.org/10.1177/1087057108328132.

Full text

Abstract:

The PI3K/AKT/mTOR pathway is central to cell growth and survival, cell cycle regulation, and programmed cell death. Aberrant activation of this signaling cascade is linked to several disease states, and thus many components of the pathway are attractive targets for therapeutic intervention. However, the considerable degree of complexity, crosstalk, and feedback regulation that exists within the pathway (especially with respect to the regulation of mTOR and its complexes) underscores the need for a comprehensive set of cell-based assays to properly identify and characterize small-molecule modul

APA, Harvard, Vancouver, ISO, and other styles

39

Stamos, James D., Lee H. Lee, Calvin Taylor, Tony Elias, and Sandra D. Adams. "In Vitro and In Silico Analysis of the Inhibitory Activity of EGCG-Stearate against Herpes Simplex Virus-2." Microorganisms 10, no. 7 (2022): 1462. http://dx.doi.org/10.3390/microorganisms10071462.

Full text

Abstract:

About half a billion people worldwide are infected with herpes simplex virus-2 (HSV-2). Prolonged treatment with acyclovir (ACV) and its analogs leads to the development of resistant strains. The aim of this study was to investigate the antiviral potential of epigallocatechin gallate (EGCG) from Camellia sinensis and a stable analog EGCG-stearate (EGCG-S) against HSV-2 in cultured Vero cells. Cell viability and cell proliferation assays were used to determine the non-cytotoxic concentrations on cultured Vero cells. HSV-2 with a green fluorescent protein (GFP) fusion protein of VP26 virions wer

APA, Harvard, Vancouver, ISO, and other styles

40

Thomas, Brad B., Yanglong Mou, Lauryn Keeler, et al. "A Highly Sensitive and Specific Gene Fusion Algorithm Based on Multiple Fusion Callers and an Ensemble Machine Learning Approach." Blood 136, Supplement 1 (2020): 12–13. http://dx.doi.org/10.1182/blood-2020-142020.

Full text

Abstract:

Background: Gene Fusion events are common occurrences in malignancies, and are frequently drivers of malignancy. FISH and qPCR are two methods often used for identifying highly prevalent gene fusions/translocations. However, these are single target assays, requiring a lot of effort and sample if multiple assays are needed for multiple targets like sarcoma. High-throughput parallel (NextGen) DNA and RNA sequencing are also in current use to detect and characterize gene fusions. RNA sequencing (RNAseq) has the advantage that multiple markers can be targeted at one time and RNA fusions are readil

APA, Harvard, Vancouver, ISO, and other styles

41

Wilkinson, Jennifer M., Steven Hayes, David Thompson, Pamela Whitney, and Kun Bi. "Compound Profiling Using a Panel of Steroid Hormone Receptor Cell-Based Assays." Journal of Biomolecular Screening 13, no. 8 (2008): 755–65. http://dx.doi.org/10.1177/1087057108322155.

Full text

Abstract:

A major focus in the current discovery of drugs targeting nuclear receptors (NRs) is identifying drugs with reduced side effects by improving selectivity, not only from other receptors but also by selective modulation of the NR of interest. Cellular assays not only provide valuable information on functional activity, potency, and selectivity but also are ideally suited for differentiating partial agonists and antagonists. The ability to partially activate a receptor is believed to be closely tied to the ability to selectively modulate the NR, resulting in expression of a subset of the normally

APA, Harvard, Vancouver, ISO, and other styles

42

Alì, Greta, Rossella Bruno, Mauro Savino, et al. "Analysis of Fusion Genes by NanoString System: A Role in Lung Cytology?" Archives of Pathology & Laboratory Medicine 142, no. 4 (2018): 480–89. http://dx.doi.org/10.5858/arpa.2017-0135-ra.

Full text

Abstract:

Context.— Patients with non–small cell lung cancer harboring ALK receptor tyrosine kinase (ALK), ROS proto-oncogene 1 (ROS1), and ret proto-oncogene (RET) gene rearrangements can benefit from specific kinase inhibitors. Detection of fusion genes is critical for determining the best treatment. Assessing rearrangements in non–small cell lung cancer remains challenging, particularly for lung cytology. Objective.— To examine the possible application of the multiplex, transcript-based NanoString system (NanoString Technologies, Seattle, Washington) in the evaluation of fusion genes in lung adenocar

APA, Harvard, Vancouver, ISO, and other styles

43

Mayer, Andreas, and William Wickner. "Docking of Yeast Vacuoles Is Catalyzed by the Ras-like GTPase Ypt7p after Symmetric Priming by Sec18p (NSF)." Journal of Cell Biology 136, no. 2 (1997): 307–17. http://dx.doi.org/10.1083/jcb.136.2.307.

Full text

Abstract:

Vacuole inheritance in yeast involves the formation of tubular and vesicular “segregation structures” which migrate into the bud and fuse there to establish the daughter cell vacuole. Vacuole fusion has been reconstituted in vitro and may be used as a model for an NSF-dependent reaction of priming, docking, and fusion. We have developed biochemical and microscopic assays for the docking step of in vitro vacuole fusion and characterized its requirements. The vacuoles must be primed for docking by the action of Sec17p (α-SNAP) and Sec18p (NSF). Priming is necessary for both fusion partners. It p

APA, Harvard, Vancouver, ISO, and other styles

44

Kagiampakis, Ioannis, Arbi Gharibi, Marie K. Mankowski, et al. "Potent Strategy To Inhibit HIV-1 by Binding both gp120 and gp41." Antimicrobial Agents and Chemotherapy 55, no. 1 (2010): 264–75. http://dx.doi.org/10.1128/aac.00376-10.

Full text

Abstract:

ABSTRACTThe development of an anti-HIV microbicide is critical in the fight against the spread of HIV. It is shown here that the covalent linking of compounds that bind gp120 with compounds that bind gp41 can inhibit HIV entry even more potently than individual inhibitors or noncovalent combinations. The most striking example involves griffithsin, a potent HIV inhibitor that binds to the surface of HIV gp120. While griffithsin inhibits HIV Env-mediated fusion in a CCR5-tropic cell-cell fusion assay with a 50% inhibitory concentration (IC50) of 1.31 ± 0.87 nM and the gp41-binding peptide C37 sh

APA, Harvard, Vancouver, ISO, and other styles

45

Marcovitz, Amir, Jeoffrey Schageman, Jian Gu, et al. "Abstract 78: Detection of gene fusions and exon skipping events in lung FFPE samples with Oncomine Precision Assay on Ion Torrent Genexus࣪ System." Cancer Research 82, no. 12_Supplement (2022): 78. http://dx.doi.org/10.1158/1538-7445.am2022-78.

Full text

Abstract:

Abstract Introduction: Gene fusions and exon skipping events play an important oncogenic role in non-small cell lung cancer (NSCLC). Here we employed the Oncomine Precision Assay (OPA) for sequencing of 998 clinical research FFPE (Formalin-Fixed Paraffin-Embedded) lung samples using the Genexus࣪ integrated sequencing platform. The RNA assay strategy is aimed at providing a wide scope for studying known oncogenic fusions and exon skip variants, as well as a method for detection of novel fusion combinations and detection of fusions in a partner agnostic manner. We summarize the findings that inc

APA, Harvard, Vancouver, ISO, and other styles

46

van der Schaar, Hilde M., Michael J. Rust, Barry-Lee Waarts, et al. "Characterization of the Early Events in Dengue Virus Cell Entry by Biochemical Assays and Single-Virus Tracking." Journal of Virology 81, no. 21 (2007): 12019–28. http://dx.doi.org/10.1128/jvi.00300-07.

Full text

Abstract:

ABSTRACT In this study, we investigated the cell entry characteristics of dengue virus (DENV) type 2 strain S1 on mosquito, BHK-15, and BS-C-1 cells. The concentration of virus particles measured by biochemical assays was found to be substantially higher than the number of infectious particles determined by infectivity assays, leading to an infectious unit-to-particle ratio of approximately 1:2,600 to 1:72,000, depending on the specific assays used. In order to explain this high ratio, we investigated the receptor binding and membrane fusion characteristics of single DENV particles in living c

APA, Harvard, Vancouver, ISO, and other styles

47

Keane, John T., and Avery D. Posey. "Abstract 4089: PanCAR-specific antibody-cytokine fusion proteins." Cancer Research 83, no. 7_Supplement (2023): 4089. http://dx.doi.org/10.1158/1538-7445.am2023-4089.

Full text

Abstract:

Abstract CAR-T cells are a potent immunotherapy that redirect T cell specificity towards cell surface tumor-associated antigens. While this therapy has been effective in hematological malignancies, therapies against solid tumors not achieved the same efficacy due to a multitude of additional challenges, including a lack of tumor specific antigens and an immunosuppressive tumor microenvironment. One strategy to increase T cell potency is to use fourth generation CAR-T cells that also secrete an immunostimulatory factor, such as a cytokine. We have created CAR-T cells targeting Tn-MUC1 utilizing

APA, Harvard, Vancouver, ISO, and other styles

48

Drew, Allison E., Samer Al-Assaad, Violeta Yu, et al. "Comparison of 2 Cell-Based Phosphoprotein Assays to Support Screening and Development of an ALK Inhibitor." Journal of Biomolecular Screening 16, no. 2 (2011): 164–73. http://dx.doi.org/10.1177/1087057110394657.

Full text

Abstract:

Anaplastic lymphoma kinase (ALK) when expressed as a fusion protein with nucleophosmin (NPM) has been implicated as a driving oncogene in a subset of lymphomas. Recent reports of ALK expression in a number of other cancers have raised the possibility that an ALK inhibitor may benefit patients with these diseases as well. In a campaign to identify and develop a selective ALK inhibitor, 2 assays were devised to measure the phosphorylation of tyrosine residue 1604 of ALK (pY1604 ALK). Amplified Luminescent Proximity Homogeneous Assay (AlphaScreen®) and phosflow platforms were used to detect modul

APA, Harvard, Vancouver, ISO, and other styles

49

Tangpeerachaikul, Anupong, Ludovic Bigot, Luc Friboulet, and Henry E. Pelish. "Abstract 3337: Preclinical activity of NVL-655 in ALK-driven cancer models beyond non-small cell lung cancer." Cancer Research 82, no. 12_Supplement (2022): 3337. http://dx.doi.org/10.1158/1538-7445.am2022-3337.

Full text

Abstract:

Abstract The ALK receptor tyrosine kinase can be aberrantly activated by gene fusions, point mutations, or amplification to promote cancer. ALK fusions are detected in approximately 5% of advanced non-small cell lung cancers (NSCLC), 50% of inflammatory myofibroblastic tumors, 50% of adult and 90% of childhood anaplastic large cell lymphomas, and rare cases of cholangiocarcinomas. Over 90 fusion partners of ALK have been identified, each exerting a different influence on the biochemical properties of the fusion protein and its preclinical sensitivity to ALK inhibitors. Besides fusions, ALK poi

APA, Harvard, Vancouver, ISO, and other styles

50

Jackson, Julia O., and Richard Longnecker. "Reevaluating Herpes Simplex Virus Hemifusion." Journal of Virology 84, no. 22 (2010): 11814–21. http://dx.doi.org/10.1128/jvi.01615-10.

Full text

Abstract:

ABSTRACT Membrane fusion induced by enveloped viruses proceeds through the actions of viral fusion proteins. Once activated, viral fusion proteins undergo large protein conformational changes to execute membrane fusion. Fusion is thought to proceed through a “hemifusion” intermediate in which the outer membrane leaflets of target and viral membranes mix (lipid mixing) prior to fusion pore formation, enlargement, and completion of fusion. Herpes simplex virus type 1 (HSV-1) requires four glycoproteins—glycoprotein D (gD), glycoprotein B (gB), and a heterodimer of glycoprotein H and L (gH/gL)—to

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!