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Ramnerö, David. "Semi-automatic Training Data Generation for Cell Segmentation Network Using an Intermediary Curator Net." Thesis, Uppsala universitet, Bildanalys och människa-datorinteraktion, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-332724.
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In this work we create an image analysis pipeline to segment cells from microscopy image data. A portion of the segmented images are manually curated and this curated data is used to train a Curator network to filter the whole dataset. The curated data is used to train a separate segmentation network to improve the cell segmentation. This technique can be easily applied to different types of microscopy object segmentation.
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Melendez, Melendez Roy Kelvin. "Sperm cell segmentation in digital micrographs based on convolutional neural networks using u-net architecture." Master's thesis, Pontificia Universidad Católica del Perú, 2021. http://hdl.handle.net/20.500.12404/19908.
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Human infertility is considered a serious disease of the the reproductive system that affects more than 10% of couples worldwide,and more than 30% of reported cases are related to men. The crucial step in evaluating male in fertility is a semen analysis, highly dependent on sperm morphology. However,this analysis is done at the laboratory manually and depends mainly on the doctor’s experience. Besides,it is laborious, and there is also a high degree of interlaboratory variability in the results. This article proposes applying a specialized convolutional neural network architecture (U-Net),which focuses on the segmentation of sperm cells in micrographs to overcome these problems.The results showed high scores for the model segmentation metrics such as precisión (93%), IoU score (86%),and DICE score of 93%. Moreover,we can conclude that U-net architecture turned out to be a good option to carry out the segmentation of sperm cells.
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Zhang, Xitong. "vU-net: edge detection in time-lapse fluorescence live cell images based on convolutional neural networks." Digital WPI, 2018. https://digitalcommons.wpi.edu/etd-theses/1224.
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Time-lapse fluorescence live cell imaging has been widely used to study various dynamic processes in cell biology. As the initial step of image analysis, it is important to localize and segment cell edges with higher accuracy. However, fluorescence live-cell images usually have issues such as low contrast, noises, uneven illumination in comparison to immunofluorescence images. Deep convolutional neural networks, which learn features directly from training images, have successfully been applied in natural image analysis problems. However, the limited amount of training samples prevents their routine application in fluorescence live-cell image analysis. In this thesis, by exploiting the temporal coherence in time-lapse movies together with VGG-16 [1] pre-trained model, we demonstrate that we can train a deep neural network using a limited number of image frames to segment the entire time-lapse movies. We propose a novel framework, vU-net, which combines the advantages of VGG-16 [1] in feature extraction and U-net [2] in feature reconstruction. Moreover, we design an auxiliary convolutional block at the end of the architecture to enhance edge detection. We evaluate our framework using dice coefficient and the distance between the predicted edge and the ground truth on high-resolution image datasets of an adhesion marker, paxillin, acquired by a Total Internal Reflection Fluorescence (TIRF) microscope. Our results demonstrate that, on difficult datasets: (i) The testing dice coefficient of vU-net is 3.2% higher than U-net with the same amount of training images. (ii) vU-net can achieve the best prediction results of U-net with one third of training images needed by U-net. (iii) vU-net produces more robust prediction than U-net. Therefore, vU-net can be more practically applied to challenging live cell movies than U-net since it requires a small size of training sets and achieved accurate segmentation.
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Liljequist, Viktor. "Development of a Bioreactor Simulator for supporting automation software test and verification." Thesis, Uppsala universitet, Avdelningen för systemteknik, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-325959.
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The GE Healthcare Life sciences organization develop and manufacture bioreactors, mixers, filtration skids and chromatography systems used together in a biomanufacturing platform. The platform is monitored and controlled by a distributed control system through a Programmable Logic Controller (PLC). The automation software controlling the platform is today tested and verified together with the physical units. The software use PROFIBUS, an industry standard for industrial automation, for communication and control of the units. Limited access to the physical units is usually a bottleneck and it's difficult to test abnormal situations to make sure the correct alarms are triggered. To reduce the hardware dependency and to provide support during test and verification, a virtual environment is developed to simulate the behavior of a bioreactor during execution. A .NET application has been developed together with a mathematical framework to simulate a cell culture and to return relevant process parameters such as pH, dissolved oxygen, temperature and weight. The results show that it's possible to simulate a bioreactor and to communicate with the control system. The software can be a valuable tool when developing and testing automation software but should not be used for process optimization or tuning of control parameters.
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Lundin, Johan. "EROI of crystalline silicon photovoltaics : Variations under different assumptions regarding manufacturing energy inputs and energy output." Thesis, Uppsala universitet, Institutionen för fysik och astronomi, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-199639.
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Installed photovoltaic nameplate power have been growing rapidly around the worldin the last few years. But how much energy is returned to society (i.e. net energy) by this technology, and which factors contribute the most to the amount of energy returned? The objective of this thesis was to examine the importance of certain inputs and outputs along the solar panel production chain and their effect on the energy return on (energy) investment (EROI) for crystalline wafer-based photovoltaics. A process-chain model was built using publicly available life-cycle inventory (LCI) datasets. This model has been kept simple in order to ensure transparency. Univariate sensitivity analysis for processes and multivariate case studies was then applied to the model. The results show that photovoltaic EROI values are very sensitive to assumptions regarding location and efficiency. The ability of solar panels to deliver net energy in northern regions of the earth is questionable. Solar cell wafer thickness have a large impact on EROI, with thinner wafers requiring less silicon material. Finding an alternative route for production of solar-grade silicon is also found to be of great importance, as is introduction of kerf loss recycling. Equal system sizes have been found to yield an primary EROI between approximately 5.5-19 depending on location and assumptions. This indicates that a generalized absolute EROI for photovoltaics may be of little use for decision-makers. Using the net energy cliff concept in relation to primary EROI found in this thesis shows that primary EROI rarely decreases to less than the threshold of 8:1 in univariate cases. Crystalline photovoltaics under similar system boundaries as those in the thesis model does not necessarily constrain economic growth on an energetic basis.
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Amghar, Bilal. "Modélisation, observabilité et commande de convertisseurs multicellulaires parallèles dans un environnement dédié." Phd thesis, Université de Cergy Pontoise, 2013. http://tel.archives-ouvertes.fr/tel-00880757.
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Les convertisseurs de puissance multicellulaires trouvent une place privilégiée dans le contrôle des systèmes de très forte puissance. Dans ce travail de thèse une nouvelle classe de convertisseurs de puissance est étudiée les Convertisseurs Multicellulaires Parallèles (CMP). La topologie de ces convertisseurs repose sur une association de n cellules de commutationinterconnectées par l'intermédiaire d'inductances indépendantes, appelées aussiinductances de liaison. Le CMP permet d'atteindre un courant de sortie égal à n fois le courant d'entrée du convertisseur, l'inconvénient majeur de ce type de convertisseur est le déséquilibrage des courants de branches . Dans le but de réduire et d'économiser le nombre de capteurs, nous avons proposé dans la première partie de la thèse une analyse d'observabilité spécifique à une classe de système dynamique hybride appelée Z(TN)-Observability et synthétisé un observateur hybride en utilisant l'algorithme super twisting. La deuxièmepartie du travail a été consacrée à la synthèse d'une loi de commande pour la régulation des courants de branches. En effet, le régulateur proposé est un régulateur hybride en basant sur la modélisation par réseaux de pétri de l'algorithme de contrôle. Enfin, Les deux parties théoriques sont suivies par une réalisation pratique d'un CMP à trois cellules de commutation pour valider les deux approches proposées. Les résultats expérimentaux nous ont montré les performances de l'observateur et le régulateur de courant et de tension de sortie.
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Scott, Lena. "Plasticity in the dopamine 1 receptor system : behavior and cell biological studies /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7140-060-5/.
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Verhoog, Nicolette Jeanette Dorothy. "Investigation of differential TNFα-induced interleukin-6 gene regulation by synthesis progestins medroxyprogesterone acetate (MPA) and norethindrone acetate (NET-A) in human endocervical epithelial cells and the role of the unliganded glucocorticoid receptor." Doctoral thesis, University of Cape Town, 2010. http://hdl.handle.net/11427/22025.
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Includes bibliographical references (pages 127-171).The endocervical mucosae of the female reproductive tract (FRT) not only serve as a physical barrier against microbial infection, but they also express a wide variety of immune mediators. The endocervical epithelial cells express a distinct profile of immune-regulators, which is higher than vaginal and ectocervical epithelial cells. Constant cytokine production would ensure rapid responses to infections and maintenance of the sterility of the upper genital tract. However, overproduction of cytokines could inhibit normal reproductive processes and stimulate excess growth and cell proliferation. The synthetic progestins medroxyprogesterone acetate (MPA) and norethisterone (NET) and its derivatives (norethisterone enanthate (NET-EN); norethisterone acetate (NET-A)) are synthetic steroidal hormones designed to elicit progestational effects similar to those of the endogenous hormone progesterone (P4). They are extensively used as contraceptives and in hormone replacement therapy (HRT). Numerous studies, however, have reported that synthetic progestins affect immune function, increase the risk of sexually transmitted infections (STIs) and also change the morphology of the cervicovaginal mucosa. Despite these findings little is known about the molecular mechanisms of action of MPA and NET, in particular their differential effects on gene expression.
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Kubín, David. "Životní cyklus solární elektrárny, efektivita a návratnost." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2013. http://www.nusl.cz/ntk/nusl-220166.
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This master’s thesis named “The Life Cycle of Solar Power, Efficiency and Return” is divided into seven chapters and focuses on the utilization of solar radiation in photovoltaic power stations and solar thermal power stations. The first chapter of this thesis familiarizes the reader with issues concerning renewable resources of energy and presents an overview of the focus of each chapter. The following second chapter is occupied with a topical research of renewable resources of energy utilization in Europe. Further the author presents a brief glance back at the past of solar energy utilization and also a prediction of future solar energy utilization in the Czech Republic. The chapter named “Specification and parameterization of individual technologies” contains an overview of today’s most utilized photovoltaic cells and panels together with an overview of utilized solar collectors and solar thermal power stations. In the following chapter named “Concretization of typical applications and realizations of photovoltaic and solar thermal power stations and determination of all related parameters” the author describes further components of photovoltaic and solar thermal systems. The economical aspect of photovoltaic component production together with an overview of utilized photovoltaic technologies is presented in this chapter. The problem of recycling photovoltaic applications and the current legislative situation regarding this issue in the Czech Republic is also outlined within this chapter. In the fifth chapter of this master’s thesis the author presents mathematical models of a photovoltaic and a solar thermal power station with the focus on economic aspects of investment efficiency assessment. Within this master’s thesis a simulation program in the computational software program Mathematica was created by the author. This program allows a calculation of economic efficiency and return of photovoltaic power station investments. The results of executed simulations are presented in the sixth chapter of this thesis. The last chapter contains an appraisal and summary of results achieved by the author of this thesis.
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Peterson, Emily. "Proteoliposome Proton Flux Assays Establish Net Conductance, pH-Sensitivity, and Functional Integrity of a Novel Truncate of the M2 Ion "Channel" of Influenza A." BYU ScholarsArchive, 2010. https://scholarsarchive.byu.edu/etd/2420.
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A novel truncate of Influenza A M2 protein (residues 22-62), incorporated into a uniquely tailored proteoliposome proton uptake assay, demonstrated proton flux more characteristic of an ion transporter than a traditional ion "channel." The liposome paradigm was essential for testing the conductance activity of this M2 truncate at a range of extraphysiological pHs appropriate for channel vs. transport function determination. In addition to transporter-typical proton flux, M2(22-62) showed the key characteristics of functional integrity: selective proton uptake into liposomes and block of uptake by amantadine. Two sets of proteoliposome proton flux assays were carried out, Set 1 at pH values of 6.5, 6.0. 5.5, 5.0, and 4.5; Set 2 at pH values of 6.25, 6.0, 5.75, 5.5, 5.25, 5.0, and 4.75. Observed flux rates followed a proton transport saturation curve similar to that observed in mouse erythroleukemia cells1. Proton transport was maximal at pH 5.5 in Set 1 (139 H+/second/tetramer) and at pH 5.75 in Set 2 (43 H+/second/tetramer). Amantadine block was strongest at pH 5.5 in Set 1 and 6.25 in Set 2, and apparent desensitization of the protein severely reduced proton flux and amantadine sensitivity below pH 5.5 in both sets of experiments. Decreased external pH increased proton uptake with an apparent pKa of 6 (Set 1) or 6.5 (Set 2). These data indicate acid activation of M2(22-62) between pH 5.5-6, optimal amantadine block between pH 5.5-6.25, and a loss of peptide functionality between pH 5.9-4.7.
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Mounier, Morgane. "Apport des méthodes de survie nette dans le pronostic des lymphomes malins non hodgkiniens en population générale." Thesis, Lyon 1, 2015. http://www.theses.fr/2015LYO10120/document.
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L'étude de la survie nette des patients atteints de cancer en population générale permet d'apprécier l'efficience globale du système de soin d'un pays. La survie nette se définit comme la survie qui serait observée si la seule cause de décès possible était le cancer. Ce concept est fondamental dans les comparaisons entre zones géographiques et/ou périodes de diagnostic dont l'intérêt est d'estimer les variations spécifiques de la mortalité due au cancer. Le concept de survie nette permet de prendre en compte les éventuelles différences de mortalité naturelle entre les groupes comparés. Actuellement, seuls deux outils estiment la survie nette sans biais : l'estimateur non paramétrique de Pohar-Perme et la modélisation paramétrique ajustée sur certaines covariables (essentiellement l'âge). Par ailleurs, les outils paramétriques s'étant perfectionnés, de nouveaux modèles flexibles permettent de modéliser les effets complexes des variables sur la mortalité. Ce travail repose sur la modélisation du taux de mortalité en excès à la suite d'un lymphome malin non hodgkinien, en se basant sur le modèle proposé par Remontet et al. et sur la nécessité de modéliser conjointement les effets complexes des covariables (telles que le temps de suivi, l'année de diagnostic et l'âge) sur la mortalité à l'aide d'une stratégie de modélisation adaptée. L'effet des variables est restitué sur la survie nette mais aussi sur le taux de mortalité en excès ce qui représente un élément nouveau dans les études de survie. Deux applications ont été menées sur des bases de données collaboratives de population : d'une part sur les données françaises du réseau FRANCIM à la suite d'un diagnostic de lymphome folliculaire entre 1995 et 2010 et, d'autre part, sur les données européennes d'EUROCARE-5 après un lymphome folliculaire ou un lymphome B diffus à grandes cellules diagnostiqué entre 1996 et 2004. Les résultats montrent que la dynamique du taux de mortalité en excès au cours du temps de suivi varie en fonction du sous-type de lymphome, de l'âge et de la zone géographique. Les tendances de cette dynamique en fonction de l'année de diagnostic sont également différentesThe net survival of cancer patients in population studies is the most relevant indicator to assess the overall efficiency of the healthcare system of a country. Net survival is defined as the survival that would be observed if the sole cause of death were cancer. This concept is crucial in comparative studies (between geographical areas and/or periods of diagnosis) that estimate specific variations of cancer-related deaths. Net survival takes into account potential differences in mortality patterns between groups. Currently, two methods provide unbiased estimations of net survival: the non-parametric estimator of Pohar-Perme and the parametric model adjusted on specific covariates (mainly, the age at diagnosis). Moreover, new improved parametric tools, such as flexible models, can model the complex covariate effects on mortality. In this work, we modeled the excess mortality rate after a non Hodgkin lymphoma diagnosis, with a model developed by Remontet et al. In addition, we used an appropriate model-building-strategy to model jointly the complex effects of some covariates (such as the time elapsed since diagnosis, the year of diagnosis, and age) on the excess mortality. Finally, this approach allowed for the covariate effects on the net survival and on the excess mortality rate. We applied this method to two different collaborative databases: first on the French database FRANCIM (1995 to 2010) to study the excess mortality after diagnosis of follicular lymphoma, then on the European data of EUROCARE-5 (1996 to 2004) to study the excess mortality after diagnosis of follicular lymphoma and diffuse large B-cell lymphoma. According to the results, the dynamics of the excess mortality rate varies over the time elapsed since diagnosis according to the lymphoma subtype, the age, and the geographical area. The trends of these dynamics over the years of diagnosis are different too
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Dandoit, Mylène. "Evaluation de l'impact de la prise en charge thérapeutique sur la survie et la qualité de vie des patients atteints d'un lymphome folliculaire ou d'un lymphome B diffus à grandes cellules." Thesis, Dijon, 2014. http://www.theses.fr/2014DIJOS038/document.
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En France, les hémopathies lymphoïdes, se situant au sixième rang des cancers les plus fréquents, sontun problème majeur de santé publique. Ce travail a pour objectif d’étudier l’impact de la prise en charge thérapeutiquesur la survie et sur la qualité de vie (QdV) des patients atteints de ce type d’hémopathies. Le premierobjectif de ce travail est un état des lieux de l’épidémiologie des hémopathies lymphoïdes avec l’étudede l’évolution de l’incidence et de la survie nette en Côte d’Or entre 1980 et 2009. L’incidence, en nette augmentationdepuis 1980, semble se stabiliser depuis les années 2000 pour certaines entités, notamment pourles lymphomes folliculaires (LF) et les lymphomes B diffus à grandes cellules (LBDGC). Nous observons globalementune amélioration de la survie nette avec, toutefois, un pronostic à court et à long terme qui restedéfavorable pour certaines entités. Les LF et les LBDGC sont les premiers lymphomes à bénéficier de l’introductiondes anticorps monoclonaux dans leur prise en charge thérapeutique. Notre deuxième étude a pourobjectif demesurer l’impact du rituximab sur la survie globale des patients atteints d’un LF ou d’un LBDGC enCôte d’Or en utilisant une méthodologie basée sur le score de propension. Nos résultats confirment le bénéficesignificatif du rituximab sur la survie globale en population générale, sans critère de sélection. En vue de cesrésultats, nous avons étudié la QdV de ces patients pendant et à la suite de la prise en charge thérapeutique. LaQdV évolue différemment au cours du suivi en fonction du type de lymphomeIn France, hematologic malignancies, which are the sixthmost common cancers, are amajor public healthproblem. This work aimed to study the impact of the therapeutic management on survival and healt-relatedquality of life (HRQoL) in patients with these hematologic malignancies. The first objective of this work is topresent an overview of the epidemiology of lymphoid malignancies with a study of changes in the incidenceand net survival in the Côte d’Or department between 1980 and 2009. The incidence, which has increased since1980, seems to have stabilized since the 2000s for some entities, including follicular lymphoma (FL) and diffuselarge B-cell lymphoma (DLBCL). Overall, we observed an improvement in net survival, with, however, a lessfavorable prognosis in the short and long-term for some entities. FL and DLBCL were the first lymphomas tobenefit from the introduction of monoclonal antibodies in their therapeutic management. Our second studyaimed to assess the impact of rituximab on overall survival in patients with FL or DLBCL in the Côte d’Or departmentusing a methodology based on the propensity score. Our results confirmed the significant benefit ofrituximab on overall survival in an unselected population of patients. In view of these results, we studied theHRQoL of these patients during and after treatment. HRQoL evolved differently during follow-up dependingon the type of lymphoma
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Leitman, Valentín. "Koncept nabíjecí stanice s možností off-grid provozu pro elektrokola." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2017. http://www.nusl.cz/ntk/nusl-319544.
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This thesis deals with charging stations for electric bicycles powered by renewable energy. The aim of this work is to make a proposal for the hybrid charging stations for electric bicycles, which will work independently and, if necessary, will be backed up by a network. In this thesis is entered theoretical information on the issue of electric bicycles, the batteries, charging stations, the connectors of chargers, and photovoltaic systems. The practical part of this thesis is the basic design of the charging station and its design of the mathematical model of the individual parts in Simulink program, which are linked to the actual design of the charging station. In conclusion, this thesis deals with energy and economic analysis of the proposed system, therein included various methods of assessing investment recommendations of appropriate processing methods and overall assessment of the subject.
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Fallah, Mohamed. "Étude et comparaison des mécanismes de tolérance à la sécheresse chez une variété résistante et une variété sensible de maïs (zea mays L. )." Clermont-Ferrand 2, 1988. http://www.theses.fr/1988CLF2S907.
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L'activite de la nitrate reductase, les teneurs glucidiques et l'assimilation nette ont ete etudiees pendant et apres la secheresse. La difference intervarietale concernant l'activite de la nitrate reductase pourrait resulter de la compartimentation cellulaire des nitrates
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Lin, Yi-Jen, and 林怡仁. "Modular Design Method for Cell Controller based on Colored Petri Net." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/wad6qy.
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碩士國立臺北科技大學
工業工程與管理系所
93
Design of Flexible Manufacturing System’s controller is important for whole factory’s operation. The good design of the controller makes production time reduce, machine’s utilization promote and parts’ conflict avoid. In order to design the good controller, it is necessary to build the modules which can simulate the manufacturing situation and configure them according to real state. In order to build the modules that can simulate the manufacturing situation, this research adopts Colored Petri Net as the construction and analysis tools. Colored Petri Net has mathematics foundation and system analysis abilities, mainly applying to the description of the cause-effect relationships among the events. The Manufacturing systems adopt the distributed control, and the control system was divided into cell level and workstation level. The cell level has the Cell Controller, and the workstation level has many group controllers. Every group was divided into many control modules to reduce the workload of the main controller. Petri Net models of many kinds of machines, such as Radio Frequency Identification module, Computer Numerically Controlled module, Material Handling System module…etc are contructed firstly. When new systems are need to design in the future, we can use the modules which have already been constructed, refine and use them to simulate the behaviors of new system. After analyzing the behaviors of systems, we can design control program based on the Logic of Petri Nets. In this paper, Visual Basic is adopted as the high-level program language to design the control program.
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Shen, Ji Tuan, and 沈基團. "Automatic petri-net generator for modeling and scheduling of flexible manufacturing cell." Thesis, 1995. http://ndltd.ncl.edu.tw/handle/19186965645752533389.
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"Near net-shape manufacturing of Nafion and Nafion/ceramic membranes for fuel cell applications." Tulane University, 2008.
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NafionRTM/ceramic nanocomposite membranes are prepared by a solid state method for fuel cell applications. Nanocomposite powders from NafionRTM pellets and a zirconium phosphate ceramic are formed by mechanical attrition. The powders are consolidated into membrane form by mechanical pressing and hot isostatic pressing. Cross-sectional analysis by scanning electron microscopy (SEM) indicates that the ceramic particles exist in agglomerates that are evenly dispersed across the membrane. Dynamic mechanical analysis (DMA) and tensile testing found the membranes to be mechanically equivalent, and in some cases superior, to a commercial extruded membrane. Increasing ceramic content is accompanied by an increase in modulus and shift in the alpha transition to higher temperature. Evaluation of membrane hydration by thermal gravimetric analysis (TGA) indicates that the prepared membranes have increased water uptake when compared to a commercially available membrane. However, as distribution of the ceramic is improved, the hydration of the sample is reduced. Low temperature differential scanning calorimetry (DSC) indicates that the additional water contributes to an increase in both freezing and nonfreezing water contents in the membranes. Proton conductivity, measured by the 4-probe method at 60°C in water as well as with varying relative humidity and temperature, revealed that the prepared membranes have conductivities that are comparable to but somewhat lower than the commercial membranes. An increase in conductivity is seen with decreased particle size and improved dispersion of the ceramicacase@tulane.edu
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Chiou, Jian Chen, and 邱建誠. "A Colored Timed Object-oriented Petri Net Approach for Developing Flexibile Manufacturing Cell Scheduling Systems." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/79207671948198313302.
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Wong, Chung-Kwun. "Characterization of the novel tetraspanin NET-7: Effects on cell behaviour and in silico predictions." 2008. http://link.library.utoronto.ca/eir/EIRdetail.cfm?Resources__ID=772104&T=F.
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Zeng, Sheng-Zhi, and 曾聖智. "Modular Design Method for Cell Controller of Reconfigurable Manufacturing System based on Colored Petri Net." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/x224eh.
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碩士國立臺北科技大學
工業工程與管理系碩士班
102
Reconfigurable Manufacturing System possess Cost-effectiveness and high- Responsiveness. RMS can increase(decrease) machine to change produce capacity for meet market demand. Beacause deadlock and conflict usually appear in system, system framework is constructed by Colored Petri Net. According to hierarchical architecture of RMS, in this study constructed CPN model, include Process Level , Cell Level and Entity Level. Each type of machine import modular design method, include Computer Numerically Controlled, Setup Station, Material Handling System and Cell Controller. If the new system will needs to design in the future, using constructed the completion module, system can be completed after continuous modification and operation. When the system not error, using the Visual Basic write a programmable logic controller. The Cell controller can achieve the goal of excellent design and manufacturing operations by the system to monitor the site, the purpose of real-time monitoring.
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Lee, Ming Rong, and 李銘榮. "The Development of An Object-Oriented Petri Net Flexible Manufacturing Cell Real Time Scheduling System." Thesis, 1997. http://ndltd.ncl.edu.tw/handle/09833484005789694676.
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碩士東海大學
工業工程學系
85
The performance of a flexible manufacturing cell (FMC) is highly dependent on the effectiveness of its scheduling system. This paper presents an approach which consists of two parts: (1) order release and resource allocation, and (2) operations scheduling. A constraint satisfaction technique (CST) is first employed to determine the appropriate order release time and allocated resource (e.g., fixture in each pallet) for each part in the order pool. Then, a colored timed object-oriented Petri net (CTOPN), which owns the characteristics of an object- oriented approach (e.g., the high degree of modularity, reusability, and maintenanability of the model), is presented to model the dynamic behavior of a FMC and to obtain the daily operations schedule for each resource (e.g., machining centers, washing machine, RGV, and loading/unloading equipments) by considering the characteristics of concurrent behavior, alternative routings, resource sharing and conflict in a FMC. Research of this CTOPN-based FMC scheduling system is sponsored by the Economic Affairs of Taiwan and has been successfully implemented in the FMC developed by the local industry in Taiwan (i.e., VICTOR Taichung Machinery Works Co., Ltd.).
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Lin, Yu-Shu, and 林宇書. "An Object-oriented Petri Net for A Dynamic Push-pop Flexible Manufacturing Cell And Its Comparative Simulation System." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/w7ys27.
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碩士逢甲大學
工業工程學所
91
This research will analyze and discuss the inner behavior of a flexible manufacturing cell in order to build up its object-oriented simulation system. An object-oriented Petri Net will be built to model a flexible manufacturing cell. The objects of the object-oriented Petri Net are directly mapped from physical objects of a flexible manufacturing cell. This cell includes a robot, several machines and a WIP buffer. The job enters the cell once the job volume is under capacity limit, therefore a dynamic scheduling system is required to schedule operations for jobs in the cell. Several common scheduling algorithms will be developed for the scheduling system. A database containing machining information is built and a simulation tool-eM-Plant will be utilized to built the simulation system for our model.
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Lin, Lan-Chi, and 林籃琪. "An Algorithm for Scheduling Flexible Manufacturing Cell Based on Hierarchical Petri Net And Construction of Simulation System for Dynamic Model." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/36nbjh.
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碩士逢甲大學
工業工程學所
90
This research studies scheduling of flexible manufacturing cell (FMC), Petri Net is employed to build a hierarchical environment for a flexible manufacturing cell. The physical layout of FMC includes：one robot、three machines of different kinds、one buffer with restricted capacity. First, we consider static scheduling of FMC, an algorithm is developed to establish-I which considers both slacks、ratio of remain operation time and operation number. When transitional routing of Petri Net commits resource conflict, transitional routine with less I will be chosen. As aresult, minimal tardiness and a small makespan are achieved. Consequently, thesimulation results are compared to the results of those using other dispatching rules. It is show that the algorithm can obtain a near-optimal solution and the searching steps willbe reduced obviously. . For the Petri Net model of FMC to be expandable, object-oriented Petri Net (OPNets) is applied to model FMC. In addition, a simulation tool-eM-Plant is utilized to simulate our system. This system can deal with different conditions with different numbers of jobs and machines. In some manufacturing environment, jobs arrive FMC in dynamic basis (in random). The algorithm developed in this research is also capable of scheduling this condition and has been applied into our simulation system. Machine breakdown and robot breakdown are also considered in the system. Finally, a static scheduling problem is implemented in our simulation system, the result shows a near-optimal solution can be reached. Additionally, dynamic scheduling problem is also implemented in this system, the results are also impressing.
24
Lin, Hong-Ren, and 林泓任. "Human adipose-derived stem cell isolation from fat tissues by membrane filtration method via nylon net filters having different pore size." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/66418766352606571075.
Full textAbstract:
碩士國立中央大學
化學工程與材料工程學系
104
Human adult stem cells, such as human adipose-derived stem cells (hADSCs), are considered to be a more attractive source of stem cells than human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). This is because human adult stem cells do not generate the ethical concerns that accompany hESCs. The hADSCs exhibit heterogeneous characteristics, indicating various genotypes and differentiation abilities. The isolated hADSCs can possess different purity levels and divergent properties depending on the purification methods used. A hybrid-membrane migration method was developed to purify hADSCs from a fat tissue solution with extremely high purity and pluripotency. A primary fat-tissue solution was permeated through the porous membranes with a pore size from 11 to 80 μm by the membrane filtration method, and the membranes were incubated in cell culture medium for 15-18 days to expand cell number and hADSCs as well as a primary fat-tissue solution were analyzed using flow-cytometry to evaluate mesenchymal stem cell surface markers. The migrated cells from the membranes were also analyzed using flow cytometry, after the membranes were cultivated in the cell culture medium after permeation of primary fat-tissue solution. The hADSCs that migrated from the membranes contained an extremely high percentage (e.g., >95%) of cells positive for mesenchymal stem cell markers. Compared with cells isolated by conventional culture method, the cells isolated by the membrane filtration method showed higher expression of several pluripotency genes (Oct4, Sox2, Klf4 and Nanog) and more calcium accumulation evaluated by alizarin red staining (ARS) and Von Kossa staining (VKS). hADSCs with high purity and pluripotency will be useful as a cell source for regenerative medicine. Especially, the effect of pore size of Nylon net filter membranes on purity and yield of the isolated hADSCs were investigated by the hybrid-membrane migration method and membrane filtration method in this study.
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Bonnet, Gilles. "Poisson hyperplane tessellation: Asymptotic probabilities of the zero and typical cells." Doctoral thesis, 2017. https://repositorium.ub.uni-osnabrueck.de/handle/urn:nbn:de:gbv:700-2017021715545.
Full textAbstract:
We consider the distribution of the zero and typical cells of a (homogeneous) Poisson hyperplane tessellation. We give a direct proof adapted to our setting of the well known Complementary Theorem. We provide sharp bounds for the tail distribution of the number of facets. We also improve existing bounds for the tail distribution of size measurements of the cells, such as the volume or the mean width. We improve known results about the generalised D.G. Kendall's problem, which asks about the shape of large cells. We also show that cells with many facets cannot be close to a lower dimensional convex body. We tacle the much less study problem of the number of facets and the shape of small cells. In order to obtain the results above we also develop some purely geometric tools, in particular we give new results concerning the polytopal approximation of an elongated convex body.