Academic literature on the topic 'Cell suspension'

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Journal articles on the topic "Cell suspension"

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Blome-Eberwein, Sigrid, Caitlin Stoudt, Hamed Amani, Sakura Helm, and Kyle Shaak. "33 Real World Cell Viability in Cell Spray Suspension." Journal of Burn Care & Research 46, Supplement_1 (2025): S28. https://doi.org/10.1093/jbcr/iraf019.033.

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Abstract Introduction Autologous epithelial cell spray, prepared with a commercial kit, is now widely used in American Burn Centers in extensive second and third degree burns where donor skin may be scarce. However, the cell viability and yield of the autologous skin suspension has not been assessed in a real-world setting and there is limited data on patient age and other demographics that may influence the number of viable cells in the suspension. The purpose of this IRB approved study was to evaluate the cell viability of an autologous skin cell suspension in a variety of age groups and Fit
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Horie, Masanori, Haruhisa Kato, Shigehisa Endoh, et al. "Effects of Various Carbon Nanotube Suspensions on A549, THP-1, and Peritoneal Macrophage Cells." Journal of Biomimetics, Biomaterials and Biomedical Engineering 24 (July 2015): 1–13. http://dx.doi.org/10.4028/www.scientific.net/jbbbe.24.1.

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The effects of iron content, fiber length, and stability of carbon nanotube (CNT) suspension on cells were examined. Five kinds of single-wall carbon nanotube (SWCNT) suspensions were prepared: with catalytic iron, without iron, long SWCNTs (stable), short SWCNTs (stable), and short SWCNT (unstable). These suspensions were applied to A549, THP-1, and mouse peritoneal macrophage cells. After a 24-h exposure, the mitochondrial activity, cell membrane damage, intracellular oxidative stress, and expression of cytokine genes were determined. Among these properties of SWCNTs, stability of CNT suspen
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E., Vishala, Vadlapudi Kumar, Ruksana F., et al. "Hemolytic saponins product enhancement in Gnidia glauca (Fresen.) Gilg cell suspension cultures." Biomedicine 43, no. 01 (2023): 291–98. http://dx.doi.org/10.51248/.v43i01.2298.

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Introduction and Aim: Gnidia glauca (Fresen.) Gilg of thymelaeaceae is enlisted as an endangered plant. Hemolytic triterpenoid saponins of oleanane and ursane classes are present in G. glauca. The purpose of the present study is to increase the production of hemolytic triterpenoids in vitro using salicylic acid as an elicitor in G. glauca cell suspensions. Materials and Methods: Explants were collected and surface sterilized, then inoculated onto Murashige and Skoog medium for callus culture, further establishing suspension cultures that were treated with salicylic acid. Saponins produced in t
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Oman, Srecko F., M. Filomena Camões, Kipton J. Powell, Raj Rajagopalan, and Petra Spitzer. "Guidelines for potentiometric measurements in suspensions Part B. Guidelines for practical pH measurements in soil suspensions (IUPAC Recommendations 2006)." Pure and Applied Chemistry 79, no. 1 (2007): 81–86. http://dx.doi.org/10.1351/pac200779010081.

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The measured cell potentials for suspension potentiometric cells have been interpreted and explained by a detailed analysis of the schemes for these cells ["Guidelines for potentiometric measurements in suspensions. Part A. The suspension effect (IUPAC Technical Report", Pure Appl. Chem.79, 67 (2007)]. Some former disagreements amongst investigations have been clarified. A new unambiguous operational definition of the suspension effect (SE) is presented. It is defined as the difference in cell potential for two suspension potentiometric cells, one with both electrodes in the separated equilibr
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Greer, Ann Francine, and Zohreh Tabaeizadeh. "Characterization and plant regeneration of cell suspension cultures of Lycopersicon chilense." Canadian Journal of Botany 69, no. 10 (1991): 2257–60. http://dx.doi.org/10.1139/b91-283.

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To produce calli for the establishment of a cell suspension, leaf, stem, and petiole explants of Lycopersicon chilense Dun., grown in vitro and in the soil, were cultured on media containing 15 different combinations of benzylaminopurine, kinetin, and indole acetic acid. Among the three types of tissues, leaf explants showed the best response. Cell suspension cultures of L. chilense were established from leaf callus derived from soil grown plants using Murashige and Skoog's medium supplemented with casein hydrolysate (250 mg/L), coconut water (5%), and 2,4-dichlorophenoxyacetic acid (2 mg/L).
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Teng, Whei-Lan, Yann-Jiun Liu, and Tai-Sen Soong. "Rapid Regeneration of Lettuce from Suspension Culture." HortScience 27, no. 9 (1992): 1030–32. http://dx.doi.org/10.21273/hortsci.27.9.1030.

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An efficient method for the regeneration of shoots directly from cell suspensions of three commercial cultivars of lettuce (Lactuca sativa L. cv. Great Lakes 659-700, Salad Bowl, and Prize Head) is described. Cell suspensions were prepared by osterizing cotyledon-derived callus for 60 seconds. The effects of callus quality, light intensity, carbohydrate type and concentration, auxins, and cytokinins on cell growth and differentiation in the suspension culture were examined. Among these factors, callus quality and carbohydrates were the most critical. The optimal medium for regeneration of shoo
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Liu, Sheng-Long, Lu Yang, Cheng-Jun Zhu, Kai Liu, Wei Han, and Jia-Feng Yao. "A method of identifying cell suspension concentration based on bioimpedance spectroscopy." Acta Physica Sinica 71, no. 7 (2022): 078701. http://dx.doi.org/10.7498/aps.71.20211837.

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Based on bioimpedance spectroscopy technology, a method of automatically identifying the cell suspension concentration is proposed. This method combines multiple linear regression algorithm and bioimpedance spectroscopy technology, which can identify the concentration of cell suspension quickly and accurately. Firstly, a strategy of random distribution of cell locations is proposed to simulate the true existence of cells. Secondly, 2400 groups of normal, cancerous and mixed cell models with different concentrations are generated by numerical simulation and their bioimpedance spectroscopy data
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Eilert, U., B. Wolters, and F. Constabel. "Ultrastructure of acridone alkaloid idioblasts in roots and cell cultures of Ruta graveolens." Canadian Journal of Botany 64, no. 6 (1986): 1089–96. http://dx.doi.org/10.1139/b86-149.

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Histological analysis of Ruta graveolens L. roots and in vitro grown cell suspensions revealed idioblasts with vacuoles containing clusters of droplets thought to be the storage compartment of acridone alkaloids. These idioblasts contained numerous vacuoles of varying sizes rather than the large, single, central vacuole characteristic of most adjacent parenchyma cells. The structure of idioblasts in roots and suspension cultures was identical. Treatment of suspension cultures with fungal elicitors known to increase alkaloid accumulation greatly did not affect the structure of idioblasts.
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Kikowska, Małgorzata, Agata Włodarczyk, Anna Stochmal, Jerzy Żuchowski, and Barbara Thiem. "Pentacyclic triterpenoids and polyphenols accumulation in cell suspension culture of Chaenomeles japonica (Thunb.) Lindl. ex Spach." Herba Polonica 65, no. 1 (2019): 1–11. http://dx.doi.org/10.2478/hepo-2019-0002.

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Summary Introduction: Callus and cell suspension cultures are widely applied in investigation of production of high-value secondary metabolites, which may be used as cosmeceuticals, nutraceuticals and pharmaceuticals. Plant cell cultures are promising alternative to intact plant sources for the production of plant-derived drugs of industrial importance. Objective: The aim of the study was to (i) initiate the cell suspension culture of Chaenomeles japonica from homogenous and uniform callus, (ii) stabilize the selected line and (iii) verify its ability to produce the desired groups of secondary
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Chen, Wusi, Jeffrey G. Norcini, Robert S. Kalmbacher, and James H. Aldrich. "Somatic Embryogenesis and Plant Regeneration of Wiregrass (Aristida stricta) and Creeping Bluestem (Schizachyrium scoparium var. stoloniferum)." HortScience 33, no. 3 (1998): 479a—479. http://dx.doi.org/10.21273/hortsci.33.3.479a.

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Initiation of callus and induction of embryogenesis were achieved from both wiregrass and creeping bluestem. MS basal medium containing coconut milk, sucrose, and 2,4-D were used to initiate callus from young inflorescence of wiregrass and creeping bluestem. The presence of 2,4-D was found to be essential for the induction and early development of embryoids, possibly up to the globular stage. In the case of bluestem, initiation of embryogenic callus required the presence of a low concentration of BA; using only 2,4-D resulted in more non-embryogenic callus. More globular embryos were formed wh
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Dissertations / Theses on the topic "Cell suspension"

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Menges, Margit. "Synchronisation of Arabidopsis cell suspension cultures." Thesis, University of Cambridge, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.620602.

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Green, Martha Alexandra. "Apoplastic ascorbate metabolism in rose cell suspension cultures." Thesis, University of Edinburgh, 2003. http://hdl.handle.net/1842/14944.

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Endogenous intraprotoplasmic ascorbate in rose cell suspension cultures as a model system ranged from 0.05 mmol kg<sup>-1</sup> in 0-d-old cultures to 1.1 mmol kg<sup>-1</sup> in 5-d-old cultures. Apoplastic ascorbate was estimated as 0.5 and 8 μM in 0- and 5-d-old cultures respectively, indicating that ascorbate is endogenous to, and may be metabolised within, the apoplast. Exogenous (apoplastic) 1 mM L-[1-<sup>14</sup>C]ascorbate was almost completely consumed (metabolised and/or taken up) by rose cultures within 8 hours of administration. Total <sup>14</sup>C was removed from medium but slo
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Chakrabortee, Sohini. "Characterisation of cytokinin responses in arabidopsis cell suspension culture." Thesis, University of Cambridge, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.613927.

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Smith, Rachel C. "Xyloglucan endotransglycosylation in the apoplast of plant cell suspension cultures." Thesis, University of Edinburgh, 1992. http://hdl.handle.net/1842/12139.

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Xyloglucan is thought to form cross-links between cellulose microfibrils within the plant cell wall. The hydrolysis of these 'tethers' may be involved in wall loosening, and a control of cell expansion. Transglycosylation reactions between xyloglucan 'tethers' has for some years been suggested as an alternative mechanism to hydrolysis by which the plant cell wall may be loosened. The work described in this thesis is an investigation of this hypothesis. Spinach (<i>Spinacia oleracea</i> L.) cell suspension cultures were incubated with [<i>xylosyl</i>-<SUP>3</SUP>H]xyloglucan nonasaccharide (XG9
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Eberhardt, Thomas Leonard. "Characterization of lignin deposition in Pinus taeda L. cell suspension cultures." Diss., This resource online, 1992. http://scholar.lib.vt.edu/theses/available/etd-07282008-134210/.

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Athwal, Gurdeep Singh. "Glutamate dehydrogenase, its role, regulation and characterisation in carrot cell suspension cultures." Thesis, University College London (University of London), 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.307584.

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Van, der Westhuizen Andries P. P. "The evaluation of solids suspension in a pilot scale mechanical flotation cell." Master's thesis, University of Cape Town, 2004. http://hdl.handle.net/11427/5390.

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Includes bibliographical references.<br>The central step in flotation is particle collection, with solids suspension together with gas dispersion and reagent mixing as necessary preconditions for particle collection. Solids suspension is therefore often identified as an important subprocess for effective flotation. Yet, surprisingly little work has been published on solids suspension in mechanical flotation cells, especially more recent studies since the advent of round mechanical flotation cells and the subsequent dramatic increases in maximum cell sizes are largely lacking.
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Riley, Bryan Scott. "The Suspension Cultivation of, and the use of Alternative Cell lines for the In Vitro Cultivation of, Treponema Pallidum Subspecies Pallidum." Thesis, University of North Texas, 1989. https://digital.library.unt.edu/ark:/67531/metadc798117/.

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This study had two objectives: to achieve suspension cultivation of Sf1Ep cells and to develop procedures for achieving the replication of T. pallidum in those cell cultures. Sf1Ep cells have been the sole cell line used for the in vitro cultivation of T. pallidum. A study was undertaken to determine if other cell lines can support growth of T. pallidum. Rabbit skin fibroblasts (RAB-9), nude mouse ear (NME) cells, and normal rebbit testis fibroblasts (RT) were compared to Sf1Ep cells for their ability to support in vitro multiplication of T. pallidum. RAB-9 cells supported multiplication of tr
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Kang, Jane. "Migration of blood cells in non-uniform suspension for a dialyzer design." Diss., Georgia Institute of Technology, 2015. http://hdl.handle.net/1853/53871.

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Hemodialysis is a renal replacement therapy that removes waste solutes from the blood stream using concentration gradients across a membrane. In order to overcome several shortcomings and increase the waste removal rate, a new dialyzer (filter) design is proposed in this study. In the new dialyzer design, the blood concurrently flows with a sheath fluid in a micro-fluidic channel. Because the blood stream directly contacts the sheath stream, it is important to prevent blood cell migration from the blood stream to the sheath stream while providing enough time for the waste solutes to diffuse
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Moraes, Trevor F. "Purification and characterization of phosphoenolpyruvate carboxylase from Brassica napus (canola) suspension cell cultures." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0001/MQ42669.pdf.

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Books on the topic "Cell suspension"

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Kowalski, Adam Jan. Microscale fluid dynamic effects in suspension processing and attrition of cell cultures. University of Birmingham, 1991.

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Ann, Salciccioli Kristina. Structural elucidation of a compound extracted from Taxus cuspidata cell suspension cultures. National Library of Canada, 1995.

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Kraml, Marlene Margaretha. Altered alkaloid metabolism in plant cell suspension cultures of Thalictrum rugosum Ait. (Ranunculaceae). National Library of Canada = Bibliothèque nationale du Canada, 1992.

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Estrella, Rosario Vera. Effect of race specific elicitors and non-specific elicitors of Cladosporium fulvum on tomato cell suspension cultures. National Library of Canada = Bibliothèque nationale du Canada, 1992.

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Thalmann, Beat. Identification of novel modulators towards high cell density and high-producing Chinese hamster ovary suspension cell cultures as well as their application in biopharmaceutical protein production. Logos Verlag, 2015.

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Swan, Timothy William. The use of higher plant cell suspension cultures in the development and assessment of In Vitro biomarker systems to detect xenobiotic exposure. University of Derby], 2004.

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Meyer, Hans-Peter, and Diego R. Schmidhalter, eds. Industrial Scale Suspension Culture of Living Cells. Wiley-VCH Verlag GmbH & Co. KGaA, 2014. http://dx.doi.org/10.1002/9783527683321.

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Vasileiadou, P. Dewatering of microbial cell suspensions using colloidal gas aphrons. UMIST, 1996.

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Radvanyi, Leslie G. Purification and characterization of calmodulin from suspension-cultured Catharanthus roseus cells. National Library of Canada, 1990.

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Kearns, Anne. Transmembrane transport of anionic fluorescent dyes by suspension-cultured plant cells. Oxford Brookes University, 1996.

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Book chapters on the topic "Cell suspension"

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Neumann, Karl-Hermann, Ashwani Kumar, and Jafargholi Imani. "Cell Suspension Cultures." In Plant Cell and Tissue Culture – A Tool in Biotechnology. Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-49098-0_4.

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Neumann, Karl-Hermann, Jafargholi Imani, and Ashwani Kumar. "Cell Suspension Cultures." In Plant Cell and Tissue Culture - A Tool in Biotechnology. Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-540-93883-5_4.

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Reddy, Jayarama. "Cell Suspension Cultures." In Plant Tissue Culture. CRC Press, 2023. http://dx.doi.org/10.1201/9781032712611-11.

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Moscatiello, Roberto, Barbara Baldan, and Lorella Navazio. "Plant Cell Suspension Cultures." In Plant Mineral Nutrients. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-152-3_5.

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Torres, Kenneth C. "Overview of Cell Suspension Culture." In Tissue Culture Techniques for Horticultural Crops. Springer US, 1989. http://dx.doi.org/10.1007/978-1-4615-9756-8_18.

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Torres, Kenneth C. "Plating of Cell Suspension Cultures." In Tissue Culture Techniques for Horticultural Crops. Springer US, 1989. http://dx.doi.org/10.1007/978-1-4615-9756-8_20.

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Longjohn, Modeline N., Hong-Dien Phan, and Sherri L. Christian. "Culturing Suspension Cancer Cell Lines." In Methods in Molecular Biology. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2376-3_2.

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Elvevold, Kjetil, Ingelin Kyrrestad, and Bård Smedsrød. "Protocol for Isolation and Culture of Mouse Hepatocytes (HCs), Kupffer Cells (KCs), and Liver Sinusoidal Endothelial Cells (LSECs) in Analyses of Hepatic Drug Distribution." In Methods in Molecular Biology. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2010-6_27.

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AbstractDevelopment of the new generation of drugs (e.g., oligo- and polynucleotides administered intravascularly either as free compounds or as nano-formulations) frequently encounters major challenges such as lack of control of targeting and/or delivery. Uncontrolled or unwanted clearance by the liver is a well-known and particularly important hurdle in this respect. Hence, reliable techniques are needed to identify the type(s) of liver cells, receptors, and metabolic mechanisms that are responsible for unwanted clearance of these compounds.We describe here a method for the isolation and cul
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Lorthois, Sylvie. "Blood suspension in a network." In Dynamics of Blood Cell Suspensions in Microflows. CRC Press, 2019. http://dx.doi.org/10.1201/b21806-8.

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Gatica-Arias, Andrés, and Alejandro Bolívar-González. "Chemical Mutagenesis of Embryogenic Cell Suspensions of Coffea arabica L. var. Catuaí Using EMS and NaN3." In Mutation Breeding in Coffee with Special Reference to Leaf Rust. Springer Berlin Heidelberg, 2023. http://dx.doi.org/10.1007/978-3-662-67273-0_7.

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AbstractChemical mutagens, such as ethyl methanesulfonate (EMS) and sodium azide (NaN3), interact with DNA and can primarily induce single base modifications along the genome. Populations derived from chemical mutagenesis experiments are presumed to harbor high density of point mutations in the genome. Therefore, this technique, along with in vitro culture methods such as somatic embryogenesis (SE), can introduce genetic variation in otherwise genetically homogeneous populations. In vitro mutagenesis of embryogenic cell suspension cultures represents an efficient method to quickly develop muta
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Conference papers on the topic "Cell suspension"

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Al-Bashir, Areen K., Lamis R. Bany Issa, and Ruba E. Khnouf. "A New Technique for Blood Cell Staining in Suspension." In 2024 Second Jordanian International Biomedical Engineering Conference (JIBEC). IEEE, 2024. https://doi.org/10.1109/jibec63210.2024.10932067.

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Merchant, Fatima, William Holmes, and Mehmet Toner. "Poloxamer 188 Enhances Functional Recovery of Lethally Heat Shocked Fibroblasts." In ASME 1997 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 1997. http://dx.doi.org/10.1115/imece1997-1329.

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Abstract Surfactant molecules, specifically triblock polymers such as Poloxamer 188 (P-188) have been shown to restore cell membrane integrity. The objective of this study was to develop functional and morphological assays to determine whether treatment with P-188 after heat shock enhances the recovery of lethally damaged cells. Human foreskin fibroblasts (HFF) were heated in suspension and cell recovery after heat shock was assessed using a functional assay based on the contraction of fibroblasts populated collagen lattices (FPCLs). Experiments were performed to evaluate the effect of various
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Liu, Jia, Yuhao Qiang, and E. Du. "Measurement of Electrical Properties of Sickle Cells From Electrical Impedance of Cell Suspension." In ASME 2017 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2017. http://dx.doi.org/10.1115/imece2017-71734.

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Analysis of the electrical properties of a biological cell can provide useful information about its characteristic features, such as the intracellular composition, charge distribution and composition changes in cell membrane, as well as the extracellular environment. Electrical impedance spectroscopy of a cell suspension can be used to extract an average measure of the electrical properties of single cells. In sickle cell disease, the disease state of a sickle red blood cell is closely related to the intracellular hemoglobin composition and concentration. This study presents an electrical impe
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Waldbillig, D., O. Kesler, Z. Tang, and A. Burgess. "Suspension Plasma Spraying of Solid Oxide Fuel Cell Electrolytes." In ITSC2007, edited by B. R. Marple, M. M. Hyland, Y. C. Lau, C. J. Li, R. S. Lima, and G. Montavon. ASM International, 2007. http://dx.doi.org/10.31399/asm.cp.itsc2007p0677.

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Abstract Suspension plasma spraying is a promising modification to traditional plasma spray techniques that may allow plasma sprayed layers with finer microstructures and better porosity control to be produced. The fine microstructures and controlled porosity of these layers, combined with plasma spraying’s ability to produce layers rapidly without requiring a post-deposition heat treatment, makes this an interesting new manufacturing method to produce solid oxide fuel cell (SOFC) active layers. This study uses an axial injection suspension plasma spray system to produce thin, high-density lay
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Yang, Yang, Rahul Mitchell Jairaj, Gaoyan Wang, et al. "Broadband Dielectric Properties Characterization of Biological Cells." In ASME 2009 Second International Conference on Micro/Nanoscale Heat and Mass Transfer. ASMEDC, 2009. http://dx.doi.org/10.1115/mnhmt2009-18508.

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A broadband characterization method for complex permittivity measurements of biological cells is presented. An algorithm for extracting permittivity of biological cells from the measured cell suspension scattering parameters is described. A coplanar wave guide (CPW) based device is fabricated and tested. DI water measurement results show good agreement with theoretical values. Yeast cell suspensions are characterized. Complex permittivity of yeast strains is extracted over the frequency range from 30 kHz to 30 GHz.
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Yoshimori, Takashi, Masaki Fukagawa, and Hiroshi Takamatsu. "Effect of Cell-to-Surface Interaction on Freeze Tolerance and Osmotic Response of Cells." In ASME 2008 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2008. http://dx.doi.org/10.1115/sbc2008-192404.

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Cryopreservation of tissues and organs, including artificial organs, could be one of the important steps in the medical service that brings the progress in the tissue engineering to realization. In this case, high viability of cryopreserved cells is critical to recovery after transplantation. In contrast, in the cryosurgery, which is expected to expand its application as a minimally invasive treatment of cancer, malignant cells should be destructed completely to prevent from recurrence. The appropriate freeze-thaw protocol is therefore needed to be established for cryopreservation or cryosurge
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Kolesovs, Sergejs, Kristaps Neiberts, and Pavels Semjonovs. "Use of microalgae Scenedesmus quadricauda and Chlorella vulgaris living-cells suspensions for plant biostimulation." In 81st International Scientific Conference of the University of Latvia. University of Latvia, 2023. http://dx.doi.org/10.22364/iarb.2023.04.

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Up to date research on microalgae as plant biostimulants remains fragmented and use of living microalgal cell suspensions is understudied. This research focuses on use of Scenedesmus quadricauda and Chlorella vulgaris living-cells suspensions as growth stimulants for garden cress (Lepidium sativum). Different concentrations of microalgal biomass suspensions were tested. It was found that plant treatment with S. quadricauda biomass 0.8 g/L (dry weight) suspension resulted in a significant improvement in L. sativum seeds germination and growth rate.
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Szołna, Alicja A., and Bronisław Grzegorzewski. "Optical analysis of red blood cell suspension." In 16th Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics. SPIE, 2008. http://dx.doi.org/10.1117/12.822390.

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Ishikawa, Takuji, T. J. Pedley, and Takami Yamaguchi. "Numerical Simulation of a Suspension of Swimming Micro-Organisms." In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-175256.

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The size of individual micro-organisms is often much smaller than that of the flow field of interest, in an oceanic plankton bloom for instance. In such cases, the suspension of micro-organisms is modelled as a continuum in which the variables are volume-averaged quantities. Continuum models for suspensions of swimming micro-organisms have been proposed for the analysis of phenomena such as bioconvection. However, the continuum models proposed so far are restricted to dilute suspensions, in which cell-cell interactions are negligible. If one wishes to analyze larger cell concentrations, it wil
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Murali, Anirudh, and Ram Rup Sarkar. "Gravitational Effect on the Cell in Biofluid Suspension." In 19th National Research Scholar s Meet 2023. Thieme Medical and Scientific Publishers Pvt. Ltd., 2024. http://dx.doi.org/10.1055/s-0044-1788204.

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Reports on the topic "Cell suspension"

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Singh, Anjali. Ultimate Guide to Automated Cell Counter: Plus Purchasing Tips. ConductScience, 2022. http://dx.doi.org/10.55157/cs20220614.

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An automated cell counter is a machine that uses either image analysis or electrical impedance principles to count cells automatically. The electrical impedance principle involves measuring changes in electrical resistance as cells pass through an aperture, while the light-scattering principle observes how cells scatter light when exposed to it. There are four main types of automated cell counting methods: Coulter Counter, Image Analysis Method, Flow Cytometry, and Stereological Cell Counting. Each method has its benefits and limitations, offering faster and more objective cell counting compar
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Mort, A. (The structure of pectins from cotton suspension culture cell walls). Office of Scientific and Technical Information (OSTI), 1990. http://dx.doi.org/10.2172/7003410.

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Mort, A. J. The structure of pectins from cotton suspension culture cell walls. Progress report. Office of Scientific and Technical Information (OSTI), 1993. http://dx.doi.org/10.2172/10104452.

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Delmer, Deborah, Nicholas Carpita, and Abraham Marcus. Induced Plant Cell Wall Modifications: Use of Plant Cells with Altered Walls to Study Wall Structure, Growth and Potential for Genetic Modification. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7613021.bard.

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Our previous work indicated that suspension-cultured plant cells show remarkable flexibility in altering cell wall structure in response either to growth on saline medium or in the presence of the cellulose synthesis inhibitor 2,-6-dichlorobenzonitrile (DCB). We have continued to analyze the structure of these modified cell walls to understand how the changes modify wall strength, porosity, and ability to expand. The major load-bearing network in the walls of DCB-adapted dicot cells that lack a substantial cellulose-xyloglucan network is comprised of Ca2+-bridged pectates; these cells also hav
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Fleetwood, James D., Elliot Slamovich, Rodney Wayne Trice, Aaron Christopher Hall, and James F. McCloskey. Doped solid oxide fuel cell electrolytes produced via combination of suspension plasma spray and very low pressure plasma spray. Office of Scientific and Technical Information (OSTI), 2012. http://dx.doi.org/10.2172/1055901.

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Arnett, Clint, Justin Lange, Ashley Boyd, Martin Page, and Donald Cropek. Expression and secretion of active Moringa oleifera coagulant protein in Bacillus subtilis. Engineer Research and Development Center (U.S.), 2021. http://dx.doi.org/10.21079/11681/41546.

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Cationic polypeptide proteins found in the seeds of the tropical plant Moringa oleifera have coagulation efficiencies similar to aluminum and ferric sulfates without their recalcitrant nature. Although these proteins possess great potential to augment or replace traditional coagulants in water treatment, harvesting active protein from seeds is laborious and not cost-effective. Here, we describe an alternative method to express and secrete active M. oleifera coagulant protein (MO) in Bacillus subtilis. A plasmid library containing the MO gene and 173 different types of secretory signal peptides
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Prusky, Dov, Noel T. Keen, and Stanley Freeman. Elicitation of Preformed Antifungal Compounds by Non-Pathogenic Fungus Mutants and their Use for the Prevention of Postharvest Decay in Avocado Fruits. United States Department of Agriculture, 1996. http://dx.doi.org/10.32747/1996.7570573.bard.

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C. gloeosporioides attacks unripe avocado fruits in the orchard. Germinated spores produce appressoria that germinate and breach the cuticle, but the resultant subcuticular hyphae become quiescent and do not develop further until fruit is harvested and ripens. Resistance of unripe avocado to attach by C. gloeosporioides is correlated with the presence of fungitoxic concentrations of the preformed antifungal compound, 1-acetoxy-2-hydroxy-4-oxoheneicosa-12, 15 diene in the pericarp of unripe fruits. The objective of this proposal was to study the signal transduction process by which elicitors in
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Levy, Avraham A., and Virginia Walbot. Regulation of Transposable Element Activities during Plant Development. United States Department of Agriculture, 1992. http://dx.doi.org/10.32747/1992.7568091.bard.

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We have studied the regulation of the maize Ac and MuDR transposable elements activities during plant development. Ac was studied in an heterologous system (transgenic tobacco plants and cell suspensions) while MuDR was studied in the native maize background. The focus of this study was on the transcriptional regulation of Ac and MuDR. For Ac, the major achievements were to show that 1-It is autoregulated in a way that the Ac-encoded transposase can repress the activity of its own promoter; 2-It is expressed at low basal level in all the plant organs that were studied, and its activity is stro
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Mawassi, Munir, Adib Rowhani, Deborah A. Golino, Avichai Perl, and Edna Tanne. Rugose Wood Disease of Grapevine, Etiology and Virus Resistance in Transgenic Vines. United States Department of Agriculture, 2003. http://dx.doi.org/10.32747/2003.7586477.bard.

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Rugose wood is a complex disease of grapevines, which occurs in all growing areas. The disease is spread in the field by vector transmission (mealybugs). At least five elongated-phloem- limited viruses are implicated in the various rugose wood disorders. The most fully characterized of these are Grapevine virus A (GV A) and GVB, members of a newly established genus, the vitivirus. GVC, a putative vitivirus, is much less well characterized than GV A or GVB. The information regarding the role of GVC in the etiology and epidemiology of rugose wood is fragmentary and no sequence data for GVC are a
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The structure of pectins from cotton suspension culture cell walls, Final report, 4/1/93-3/31/96. Office of Scientific and Technical Information (OSTI), 1996. http://dx.doi.org/10.2172/486590.

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