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Journal articles on the topic 'Cell suspension'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Blome-Eberwein, Sigrid, Caitlin Stoudt, Hamed Amani, Sakura Helm, and Kyle Shaak. "33 Real World Cell Viability in Cell Spray Suspension." Journal of Burn Care & Research 46, Supplement_1 (2025): S28. https://doi.org/10.1093/jbcr/iraf019.033.

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Abstract Introduction Autologous epithelial cell spray, prepared with a commercial kit, is now widely used in American Burn Centers in extensive second and third degree burns where donor skin may be scarce. However, the cell viability and yield of the autologous skin suspension has not been assessed in a real-world setting and there is limited data on patient age and other demographics that may influence the number of viable cells in the suspension. The purpose of this IRB approved study was to evaluate the cell viability of an autologous skin cell suspension in a variety of age groups and Fit

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Horie, Masanori, Haruhisa Kato, Shigehisa Endoh, et al. "Effects of Various Carbon Nanotube Suspensions on A549, THP-1, and Peritoneal Macrophage Cells." Journal of Biomimetics, Biomaterials and Biomedical Engineering 24 (July 2015): 1–13. http://dx.doi.org/10.4028/www.scientific.net/jbbbe.24.1.

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The effects of iron content, fiber length, and stability of carbon nanotube (CNT) suspension on cells were examined. Five kinds of single-wall carbon nanotube (SWCNT) suspensions were prepared: with catalytic iron, without iron, long SWCNTs (stable), short SWCNTs (stable), and short SWCNT (unstable). These suspensions were applied to A549, THP-1, and mouse peritoneal macrophage cells. After a 24-h exposure, the mitochondrial activity, cell membrane damage, intracellular oxidative stress, and expression of cytokine genes were determined. Among these properties of SWCNTs, stability of CNT suspen

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E., Vishala, Vadlapudi Kumar, Ruksana F., et al. "Hemolytic saponins product enhancement in Gnidia glauca (Fresen.) Gilg cell suspension cultures." Biomedicine 43, no. 01 (2023): 291–98. http://dx.doi.org/10.51248/.v43i01.2298.

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Introduction and Aim: Gnidia glauca (Fresen.) Gilg of thymelaeaceae is enlisted as an endangered plant. Hemolytic triterpenoid saponins of oleanane and ursane classes are present in G. glauca. The purpose of the present study is to increase the production of hemolytic triterpenoids in vitro using salicylic acid as an elicitor in G. glauca cell suspensions. Materials and Methods: Explants were collected and surface sterilized, then inoculated onto Murashige and Skoog medium for callus culture, further establishing suspension cultures that were treated with salicylic acid. Saponins produced in t

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Oman, Srecko F., M. Filomena Camões, Kipton J. Powell, Raj Rajagopalan, and Petra Spitzer. "Guidelines for potentiometric measurements in suspensions Part B. Guidelines for practical pH measurements in soil suspensions (IUPAC Recommendations 2006)." Pure and Applied Chemistry 79, no. 1 (2007): 81–86. http://dx.doi.org/10.1351/pac200779010081.

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The measured cell potentials for suspension potentiometric cells have been interpreted and explained by a detailed analysis of the schemes for these cells ["Guidelines for potentiometric measurements in suspensions. Part A. The suspension effect (IUPAC Technical Report", Pure Appl. Chem.79, 67 (2007)]. Some former disagreements amongst investigations have been clarified. A new unambiguous operational definition of the suspension effect (SE) is presented. It is defined as the difference in cell potential for two suspension potentiometric cells, one with both electrodes in the separated equilibr

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Greer, Ann Francine, and Zohreh Tabaeizadeh. "Characterization and plant regeneration of cell suspension cultures of Lycopersicon chilense." Canadian Journal of Botany 69, no. 10 (1991): 2257–60. http://dx.doi.org/10.1139/b91-283.

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To produce calli for the establishment of a cell suspension, leaf, stem, and petiole explants of Lycopersicon chilense Dun., grown in vitro and in the soil, were cultured on media containing 15 different combinations of benzylaminopurine, kinetin, and indole acetic acid. Among the three types of tissues, leaf explants showed the best response. Cell suspension cultures of L. chilense were established from leaf callus derived from soil grown plants using Murashige and Skoog's medium supplemented with casein hydrolysate (250 mg/L), coconut water (5%), and 2,4-dichlorophenoxyacetic acid (2 mg/L).

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Teng, Whei-Lan, Yann-Jiun Liu, and Tai-Sen Soong. "Rapid Regeneration of Lettuce from Suspension Culture." HortScience 27, no. 9 (1992): 1030–32. http://dx.doi.org/10.21273/hortsci.27.9.1030.

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An efficient method for the regeneration of shoots directly from cell suspensions of three commercial cultivars of lettuce (Lactuca sativa L. cv. Great Lakes 659-700, Salad Bowl, and Prize Head) is described. Cell suspensions were prepared by osterizing cotyledon-derived callus for 60 seconds. The effects of callus quality, light intensity, carbohydrate type and concentration, auxins, and cytokinins on cell growth and differentiation in the suspension culture were examined. Among these factors, callus quality and carbohydrates were the most critical. The optimal medium for regeneration of shoo

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7

Liu, Sheng-Long, Lu Yang, Cheng-Jun Zhu, Kai Liu, Wei Han, and Jia-Feng Yao. "A method of identifying cell suspension concentration based on bioimpedance spectroscopy." Acta Physica Sinica 71, no. 7 (2022): 078701. http://dx.doi.org/10.7498/aps.71.20211837.

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Based on bioimpedance spectroscopy technology, a method of automatically identifying the cell suspension concentration is proposed. This method combines multiple linear regression algorithm and bioimpedance spectroscopy technology, which can identify the concentration of cell suspension quickly and accurately. Firstly, a strategy of random distribution of cell locations is proposed to simulate the true existence of cells. Secondly, 2400 groups of normal, cancerous and mixed cell models with different concentrations are generated by numerical simulation and their bioimpedance spectroscopy data

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Eilert, U., B. Wolters, and F. Constabel. "Ultrastructure of acridone alkaloid idioblasts in roots and cell cultures of Ruta graveolens." Canadian Journal of Botany 64, no. 6 (1986): 1089–96. http://dx.doi.org/10.1139/b86-149.

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Histological analysis of Ruta graveolens L. roots and in vitro grown cell suspensions revealed idioblasts with vacuoles containing clusters of droplets thought to be the storage compartment of acridone alkaloids. These idioblasts contained numerous vacuoles of varying sizes rather than the large, single, central vacuole characteristic of most adjacent parenchyma cells. The structure of idioblasts in roots and suspension cultures was identical. Treatment of suspension cultures with fungal elicitors known to increase alkaloid accumulation greatly did not affect the structure of idioblasts.

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Kikowska, Małgorzata, Agata Włodarczyk, Anna Stochmal, Jerzy Żuchowski, and Barbara Thiem. "Pentacyclic triterpenoids and polyphenols accumulation in cell suspension culture of Chaenomeles japonica (Thunb.) Lindl. ex Spach." Herba Polonica 65, no. 1 (2019): 1–11. http://dx.doi.org/10.2478/hepo-2019-0002.

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Summary Introduction: Callus and cell suspension cultures are widely applied in investigation of production of high-value secondary metabolites, which may be used as cosmeceuticals, nutraceuticals and pharmaceuticals. Plant cell cultures are promising alternative to intact plant sources for the production of plant-derived drugs of industrial importance. Objective: The aim of the study was to (i) initiate the cell suspension culture of Chaenomeles japonica from homogenous and uniform callus, (ii) stabilize the selected line and (iii) verify its ability to produce the desired groups of secondary

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10

Chen, Wusi, Jeffrey G. Norcini, Robert S. Kalmbacher, and James H. Aldrich. "Somatic Embryogenesis and Plant Regeneration of Wiregrass (Aristida stricta) and Creeping Bluestem (Schizachyrium scoparium var. stoloniferum)." HortScience 33, no. 3 (1998): 479a—479. http://dx.doi.org/10.21273/hortsci.33.3.479a.

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Initiation of callus and induction of embryogenesis were achieved from both wiregrass and creeping bluestem. MS basal medium containing coconut milk, sucrose, and 2,4-D were used to initiate callus from young inflorescence of wiregrass and creeping bluestem. The presence of 2,4-D was found to be essential for the induction and early development of embryoids, possibly up to the globular stage. In the case of bluestem, initiation of embryogenic callus required the presence of a low concentration of BA; using only 2,4-D resulted in more non-embryogenic callus. More globular embryos were formed wh

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Sharaf Eldin, Osama, Mahmoud M. Elfar, and Abdel-Motaal Fouda. "Assessment of Global Methylation in Paraffin Embedded Prostatic Tissues and Cell Lines Using Flow Cytometry." Advances in Medicine and Medical Research 1, no. 1 (2018): 34–38. http://dx.doi.org/10.31377/ammr.v1i1.495.

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Background. The aim of this study is to measure global 5-methylcystosine (5MeC) methylation in paraffin embedded prostatic tissues and cell lines using flow cytometry. Methods. Cell/nuclei suspension from 10 cases of benign prostatic hyperplasia (BPH), 10 cases of prostatic adenocarcinoma, and two prostatic cell lines (PNT1A and LNCaP) were prepared using modified heat pretreatment technique. 5MeC global methylation was assessed by flow cytometry of cell/nuclei suspension and immunostaining of tissue sections. Results. Higher percentage of positively stained cells (PPSC) and mean channel fluor

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Ketchart, O., A. Treetong, P. Na-Ubon, and N. Supaka. "Determination the Effect of Silver Nanoparticles on Gram-Positive Bacterial Cells by Atomic Force Microscopy." Advanced Materials Research 506 (April 2012): 202–5. http://dx.doi.org/10.4028/www.scientific.net/amr.506.202.

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The atomic force microscope (AFM) was employed to study the significant effects of silver (Ag) nanoparticles-treated on the elastic cell wall of bacteria. In this study, the exposed Staphylococcus aureus was grown at 37 °C for 14 h. The cultures were centrifuged and cell pellets were resuspended in Milli-Q water to prepare final bacterial suspensions. A drop of bacterial suspension was deposited on polydimethylsiloxane (PDMS) sheet and allowed to air dry at room temperature before imaging. The cell suspension was collected at certain time intervals from the beginning of the test. The morpholog

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Stano, J., K. Mičieta A Barth, M. Valšíková, M. Fulmeková, P. Matejka, and M. Varadínová. "Identification of sucrase activity in cell suspension and culture medium of melon." Horticultural Science 32, No. 3 (2011): 104–7. http://dx.doi.org/10.17221/3774-hortsci.

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The activity of (soluble acid) sucrase was detected in a culture medium of the cell suspension culture of watermelon (Citrullus vulgaris L.). A&nbsp;simple and rapid procedure for the identification and determination of extracellular sucrase from a&nbsp;culture medium of watermelon cell suspension cultures is described. Sucrose was used as a substrate for the determination of extracellular and intracellular activities of the enzyme. Intracellular activity was estimated from the cell suspension. The results show a 91.5&ndash;92.0% intracellular and 8.0&ndash;8.5% extracellular d

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Jones, K. H., and J. A. Senft. "An improved method to determine cell viability by simultaneous staining with fluorescein diacetate-propidium iodide." Journal of Histochemistry & Cytochemistry 33, no. 1 (1985): 77–79. http://dx.doi.org/10.1177/33.1.2578146.

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A rapid, simultaneous double-staining procedure using fluorescein diacetate (FDA) and propidium iodide (PI) is described for use in the determination of cell viability in cell suspension. Air-dried slide preparations can be made from the cell suspensions so that an accurate estimate of the viability of the cells in the original suspension can be made up to 1 week later. Viable cells fluoresce bright green, while nonviable cells are bright red. Furthermore, when FDA-PI staining is compared to trypan blue dye exclusion as a method to determine cell viability, FDA-PI is found to be more consisten

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MacMECCAN, ROBERT M., J. R. CLAUSEN, G. P. NEITZEL, and C. K. AIDUN. "Simulating deformable particle suspensions using a coupled lattice-Boltzmann and finite-element method." Journal of Fluid Mechanics 618 (January 10, 2009): 13–39. http://dx.doi.org/10.1017/s0022112008004011.

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A novel method is developed to simulate suspensions of deformable particles by coupling the lattice-Boltzmann method (LBM) for the fluid phase to a linear finite-element analysis (FEA) describing particle deformation. The methodology addresses the need for an efficient method to simulate large numbers of three-dimensional and deformable particles at high volume fraction in order to capture suspension rheology, microstructure, and self-diffusion in a variety of applications. The robustness and accuracy of the LBM–FEA method is demonstrated by simulating an inflating thin-walled sphere, a deform

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Mueller-Klieser, W., R. Zander, and P. Vaupel. "A new photometric method for oxygen consumption measurements in cell suspensions." Journal of Applied Physiology 61, no. 2 (1986): 449–55. http://dx.doi.org/10.1152/jappl.1986.61.2.449.

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A new technique is described for measuring O2 consumption rates and O2 concentrations in suspensions of respiring cells. Aliquots of a cell suspension kept in a special thermostated precision syringe are injected into the measuring system in defined time intervals. The O2 content of these samples is determined photometrically, as reported previously. The O2 consumption per cellular wet weight and/or per single cell can be calculated from the cell volume fraction, the physical density, the cell concentration in the suspension, and the time-dependent decline of the O2 concentration in the precis

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Rohela, Gulab Khan, Prasad Bylla, Sreenu Pendli, Rajender Korra, Rajender Gandu, and Christopher Reuben. "High performance liquid chromatography based quantification of reserpine in Rauwolfia tetraphylla L. and enhanced production through precursor feeding." Acta Chromatographica 34, no. 2 (2021): 120–29. http://dx.doi.org/10.1556/1326.2021.00888.

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Abstract Rauwolfia tetraphylla L., is an important medicinal plant in Apocynaceae family and is recognized as an alternative source to Rauwolfia serpentina L., in terms of anti-hypertensive alkaloid production i.e. reserpine. In view of this, the present study is conducted to estimate the reserpine content in different parts (leaf, stem and root) of field grown plants (2 years old), tissue cultured plantlets (R1) (two months old) and cell suspensions cultures (two months old with and without precursor feeding) of R. tetraphylla by using high performance liquid chromatography (HPLC) technique.

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Furmanowa, Mirosława, and Lucyna Rapczewska. "Cell suspension culture of Amsonia tabernaemontana Walter: growth, organogenesis and alkaloid production." Acta Societatis Botanicorum Poloniae 50, no. 4 (2014): 615–24. http://dx.doi.org/10.5586/asbp.1981.083.

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The paper discusses the growth of cell suspension cultures of <em>Amsonia tabernaemontana</em> Walter established from callus of hypocotyl origin. The cell number and growth increment were determined. Cellular aggregates developed well in the Wood and Braun (WB) medium with 1 mg/l NAA and 0.5 mg/l kinetin (growth increment 712.4). When the aggregates were cultured on WB media without NAA and kinetin or with 0.02 mg/l kinetin and 3 mg/l IAA, Toots developed an the aggregates. Examiination of the roots and cell suspensions indicates that the Toots are richer in alkaloids than the cal

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Kaiser, Stephan C., Katharina Blaschczok, and Dieter Eibl. "Novel CHO Suspension Cell Cultivation." Genetic Engineering & Biotechnology News 33, no. 14 (2013): 32–33. http://dx.doi.org/10.1089/gen.33.14.17.

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Sherwood, J. D. "Cell models for suspension viscosity." Chemical Engineering Science 61, no. 20 (2006): 6727–31. http://dx.doi.org/10.1016/j.ces.2006.07.016.

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Curtis, Wayne R., and Alden H. Emery. "Plant cell suspension culture rheology." Biotechnology and Bioengineering 42, no. 4 (1993): 520–26. http://dx.doi.org/10.1002/bit.260420416.

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Aloysius, Morris Terfa, Eleni Andreou, Kyriacos Felekkis, C. Petrou, and Chidi Evans Egwim. "Determination of viability in yeast cell culture for the study of some plant base insulin releasing and insulin like activity therapy plants (Abelmoschus esculentus L., Musa paradisiaca, and Dioscorea dumetorum)." European Journal of Biology and Medical Science Research 12, no. 3 (2024): 66–92. https://doi.org/10.37745/ejbmsr.2013/vol12n36692.

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The determination of viability in yeast cell culture for the study of some insulin – releasing and insulin – like activity therapy plants (Abelmoschus esculentus L., Musa paradisiaca, and Dioscorea dumetorum) was studied using standard techniques, and data analytical tools respectively. The yeast cell line was used for this study. Yeast cell measurement at OD600 was carried out with the assistance of a spectrophotometer. Yeast cell counting was done first with a hemocytometer to determine the yeast cell number (as a correlation between the OD600). The yeast cell was very viable 95.9%. The tryp

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Pereira-Dias, Francis, Neusa Steiner, Gabriela Cangahaula-Inocente, Ana Paula Lando, Marisa Santos, and Miguel Pedro Guerra. "Integrated proteomics and histochemical analysis of Araucaria angustifolia (Bertol.) Kuntze (Araucariaceae) in embryogenic suspension culture." Annals of Forest Research 63, no. 2 (2020): 27–43. http://dx.doi.org/10.15287/afr.2020.1918.

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t Cell suspension culture is a useful in vitro model-system for both scaling up and conserving the Brazilian conifer Araucaria angustifolia. In the present work, cell suspension of Araucaria was subjected to proteomics, biochemical and histochemical analyses. The results revealed new insights underlying the molecular mechanism of proembryogenic masses transition in cell suspension. Embryogenic cell cultures were cultivated in a basal liquid medium modified in a Steward apparatus (orbital agitator). Cell growth dynamics was evaluated using cell volume after sedimentation, fresh weight, mitotic

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Tung, Nguyen Thanh, Thi Pham Thi Diem, Dang Ngoc Sang, Do Thi Thao, and Hoang Tan Quang. "Biomass Accumulation of Gynostemma Pentaphyllum (Thunb.) Makino in Cell Suspension Cultures inhibiting Human Cancer Cell Growth." Research Journal of Biotechnology 17, no. 3 (2022): 61–68. http://dx.doi.org/10.25303/1703rjbt6168.

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Gynostemma pentaphyllum (Thunb.) Makino (GpM) is a medicinal plant in traditional medicine throughout Asia for the treatment of several diseases including cancer. GpM plant cell suspension cultures provide a time and cost effective well-controlled means promising a high-yielding biomass production of pharmaceutical compounds. The purpose of the current work is to investigate the effect of GpM cell suspension cultures on human cancer cell lines growth. The biomass was produced by cell suspension culture of GpM callus into 250 mL Erlenmeyer flask containing 50 mL of liquid medium culture. Gypeno

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Pless-Petig, Gesine, Björn Walter, Anja Bienholz, and Ursula Rauen. "Mitochondrial Impairment as a Key Factor for the Lack of Attachment after Cold Storage of Hepatocyte Suspensions." Cell Transplantation 26, no. 12 (2017): 1855–67. http://dx.doi.org/10.1177/0963689717743254.

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Isolated primary hepatocytes, which are widely used for pharmacological and clinical purposes, usually undergo certain periods of cold storage in suspension during processing. While adherent hepatocytes were shown previously to suffer iron-dependent cell death during cold (4 °C) storage and early rewarming, we previously found little iron-dependent hepatocyte death in suspension but severely decreased attachment ability unless iron chelators were added. Here, we focus on the role of mitochondrial impairment in this nonattachment of hepatocyte suspensions. Rat hepatocyte suspensions were stored

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Reinke, W., P. Gaehtgens, and P. C. Johnson. "Blood viscosity in small tubes: effect of shear rate, aggregation, and sedimentation." American Journal of Physiology-Heart and Circulatory Physiology 253, no. 3 (1987): H540—H547. http://dx.doi.org/10.1152/ajpheart.1987.253.3.h540.

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Apparent viscosity was determined in vertical glass tubes (ID 30.2-132.3 microns) with suspensions of human red cells in A) serum, B) saline containing 0.5 g/100 ml albumin, C) plasma, and D) plasma containing Dextran 250 at a feed hematocrit of 0.45. Pressure-flow relationships were obtained in a range of pseudo-shear rates (mu) between 0.15 and 250 s-1. Relative viscosities in the nonaggregating suspensions (A and B) were found to increase monotonically with decreasing mu. The Fahraeus-Lindqvist effect was present in the entire range of mu. In the two aggregating suspensions (C and D), visco

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Krupska, T. V., V. V. Turov, M. D. Tsapko, J. Skubishevska-Zieba, and L. Leboda. "Encapsulation of cellular suspensions of lactic bacteria with silica." Himia, Fizika ta Tehnologia Poverhni 12, no. 1 (2021): 58–66. http://dx.doi.org/10.15407/hftp12.01.058.

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A promising direction for long-term storage of cells at relatively high temperatures may be their encapsulation with nanoscale biologically inert materials capable of creating a shell around microdroplets of a cell suspension, which, on the one hand, provides the possibility of gas exchange between the suspension and the external environment, and on the other hand, inhibits the processes of cell life, so transferring them to a state close to suspended animation. The method of low-temperature 1H NMR spectroscopy was used to study the process of hydration of lactobacilli, the effect of a weakly

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Ciobanu, Steluta Carmen, Simona Liliana Iconaru, Daniela Predoi, Alina Mihaela Prodan, and Mihai Valentin Predoi. "Physico-Chemical Properties and In Vitro Antifungal Evaluation of Samarium Doped Hydroxyapatite Coatings." Coatings 10, no. 9 (2020): 827. http://dx.doi.org/10.3390/coatings10090827.

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Hydroxyapatite (HAp) and samarium doped hydroxyapatite, Ca10−xSmx(PO4)6(OH)2, xSm = 0.05, (5SmHAp), coatings were prepared by sol-gel process using the dip coating method. The stability of 5SmHAp suspension was evaluated by ultrasound measurements. Fourier transform infrared spectroscopy (FTIR) was used to examine the optical characteristics of HAp and 5SmHAp nanoparticles in suspension and coatings. The FTIR analysis revealed the presence of the functional groups specific to the structure of hydroxyapatite in the 5SmHAp suspensions and coatings. The morphology of 5SmHAp nanoparticles in suspe

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Craig, B., L. Hawkey, and A. LeFurgey. "Techniques for cryoultramicrotomy of propane jet frozen biological samples." Proceedings, annual meeting, Electron Microscopy Society of America 44 (August 1986): 260–61. http://dx.doi.org/10.1017/s042482010014292x.

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Ultra-rapid freezing followed by cryoultramicrotomy is essential for the preservation of diffusible elements in situ within cells prior to scanning transmission electron microscopy and quantitative energy dispersive x-ray microanalysis. For cells or tissue fragments in suspension and for monolayer cell cultures, propane jet freezing provides cooling rates greater than 30,000°C/sec with regions up to 40μm in thickness free of significant ice crystal formation. While this method of freezing has frequently been applied prior to freeze fracture or freeze substitution, it has not been widely utiliz

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Djordjevic, Bozidar, and Christopher S. Lange. "Cell-cell interactions in spheroids maintained in suspension." Acta Oncologica 45, no. 4 (2006): 412–20. http://dx.doi.org/10.1080/02841860500520743.

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Nie, Minghao, Shogo Nagata, Hoshimi Aoyagi, Akane Itou, Ai Shima та Shoji Takeuchi. "Cell-laden microfibers fabricated using μl cell-suspension". Biofabrication 12, № 4 (2020): 045021. http://dx.doi.org/10.1088/1758-5090/ab89cb.

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Calderan-Rodrigues, Maria Juliana, Elisabeth Jamet, Maria Beatriz Calderan Rodrigues Bonassi, et al. "Cell wall proteomics of sugarcane cell suspension cultures." PROTEOMICS 14, no. 6 (2014): 738–49. http://dx.doi.org/10.1002/pmic.201300132.

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Stappers, Linda, Li Zhang, Omer Van der Biest, and Jan Fransaer. "Study of the Deposit Resistance during Electrophoretic Deposition." Key Engineering Materials 412 (June 2009): 9–14. http://dx.doi.org/10.4028/www.scientific.net/kem.412.9.

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Deposition experiments in a Hull cell showed that high conductivity suspensions yield uniform deposits while low conductivity suspensions result in non-uniform deposits. This difference in deposition behavior is related to the resistance increase of the deposit during EPD. Impedance measurements during EPD showed that the ratio of the deposit resistance to the suspension resistance increases much more for high than for the low conductivity suspensions. They also showed that the total resistance of the EPD cell dropped almost to the suspension resistance after the electric field was turned off.

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Rybczyński, Jan J., Andrzej Kaźmierczak, Katarzyna Dos Santos Szewczyk, Wojciech Tomaszewicz, Małgorzata Miazga-Karska, and Anna Mikuła. "Biotechnology of the Tree Fern Cyathea smithii (J.D. Hooker 1854; Soft Tree Fern, Katote). II. Cell Suspension Culture: Focusing on Structure and Physiology in the Presence of 2,4-D and BAP." Cells 11, no. 9 (2022): 1396. http://dx.doi.org/10.3390/cells11091396.

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The aim of our research was to describe the structure and growth potential of a cell suspension of the tree fern Cyathea smithii. Experiments were performed on an established cell suspension with ½ MS medium supplemented with 9.05 µM 2,4-D + 0.88 µM BAP. In the experiments, attention was paid to the microscopic description of cell suspension, evaluation of cell growth dependent on the initial mass of cells and organic carbon source in the medium, the length of the passage, the content of one selected flavonoid in the post-culture medium, nuclear DNA content, ethylene production, and the antimi

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Hotter, Grant S. "Elicitor-induced Oxidative Burst and Phenylpropanoid Metabolism in Pinus radiata Cell Suspension Cultures." Functional Plant Biology 24, no. 6 (1997): 797. http://dx.doi.org/10.1071/pp96094.

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A cell wall elicitor preparation from the needle pathogen Dothistroma pini was used to induce defence responses in Pinus radiata cell suspension cultures. Addition of elicitor to cell suspensions induced a rapid, transient burst in the accumulation of H2O2, with maximal response between 20 and 40 min post-elicitation. The protein kinase inhibitors staurosporine and K252a inhibited H2O2 accumulation showing that protein phosphorylation is required in the signal transduction pathway leading to the oxidative burst. Over a more extended time period elicitation of suspension cells lead to the activ

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LUPPENS, S. B. I., F. M. ROMBOUTS, and T. ABEE. "The Effect of the Growth Phase of Staphylococcus aureus on Resistance to Disinfectants in a Suspension Test." Journal of Food Protection 65, no. 1 (2002): 124–29. http://dx.doi.org/10.4315/0362-028x-65.1.124.

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The influence of growth phase on the resistance of Staphylococcus aureus to the surface-active agents benzalkonium chloride and dodecylbenzyl sulfonic acid and the oxidizing agents sodium hypochlorite and hydrogen peroxide was studied. The resistances of cells in different growth phases were compared to those of solid medium cells grown according to the European phase 1 suspension test. Using cells from different growth phases (±3 × 107 CFU ml−1), we found that decline-phase cells were the most resistant cells. However, the decline-phase cell suspension contained more than 90% dead cells. A 10

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Morais-Lino, Lucymeire Souza, Janay Almeida dos Santos-Serejo, Sebastião de Oliveira e. Silva, José Raniere Ferreira de Santana, and Adilson Kenji Kobayashi. "Cell suspension culture and plant regeneration of a Brazilian plantain, cultivar Terra." Pesquisa Agropecuária Brasileira 43, no. 10 (2008): 1325–30. http://dx.doi.org/10.1590/s0100-204x2008001000010.

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The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male flowers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic embryos. Five semisolid culture media were tested for differentiation, maturation, somatic embryos germination and for plant regeneration. An average of 558 plants per one milliliter of 5% SCV (settled cell

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Kumar, Vadlapudi. "Phytocompounds Profiling in Cell Suspensions Extracts of Gnidia glauca (Fresen.) Gilg." Indian Journal of Pure & Applied Biosciences 11, no. 2 (2023): 23–29. http://dx.doi.org/10.18782/2582-2845.8960.

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Phytocompounds enable plants to play a remarkable role as traditional remedies for human ailments. Due to their extremely low side effects, the ancient herbal medicinal systems are more beneficial than the modern medical systems. Gnidia glauca (Fresen.) Gilg has many biological properties, including antifungal, anticancer, antibacterial, and traditional medicine for swelling, indigestion, and snake bites. Because of the plant's impressive qualities and status as an endangered plant species, the callus suspension method was used as an alternative technique for phytocompounds enhancement. In ord

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Gadzovska-Simic, Sonja, O. Tusevski, S. Antevski, et al. "Secondary metabolite production in Hypericum perforatum L. cell suspensions upon elicitation with fungal mycelia from Aspergillus flavus." Archives of Biological Sciences 64, no. 1 (2012): 113–21. http://dx.doi.org/10.2298/abs1201113g.

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We investigated the production of phenylpropanoids (phenolic compounds, flavanols, flavonols and anthocyanins) and naphtodianthrones (hypericins) in elicited Hypericum perforatum L. cell suspensions. To determine whether secondary metabolite production could be enhanced, Hypericum cell suspensions were exposed to mycelia extract from the fungus Aspergillus flavus. Elicited Hypericum cell suspension cultures displayed reduced growth and viability and a modification of secondary metabolites production. Anthocyanins were only stimulated in fungal-elicited cell suspensions. Secondary metabolite pr

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Predoi, Daniela, Simona Liliana Iconaru, and Mihai Valentin Predoi. "Fabrication of Silver- and Zinc-Doped Hydroxyapatite Coatings for Enhancing Antimicrobial Effect." Coatings 10, no. 9 (2020): 905. http://dx.doi.org/10.3390/coatings10090905.

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This study develops, for the first time, composite coatings based on silver and zinc doped hydroxyapatite in chitosan matrix (AgZnHApCs). The AgZnHApCs composite coatings were prepared by dip coating method. The hydroxyapatite (HAp), biocompatible material for regenerating and strengthening damaged bones were doped with silver and zinc ions and coated with chitosan in order to produce a uniform and homogenous coating with biocompatibility and antimicrobial properties. The stability of AgZnHApCs suspensions was evaluated by ultrasound measurements. The value of stability parameters of AgZnHApCs

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Janoschek, F., F. Mancini, J. Harting, and F. Toschi. "Rotational behaviour of red blood cells in suspension: a mesoscale simulation study." Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences 369, no. 1944 (2011): 2337–44. http://dx.doi.org/10.1098/rsta.2011.0086.

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The nature of blood as a suspension of red blood cells makes computational haemodynamics a demanding task. Our coarse-grained blood model, which builds on a lattice Boltzmann method for soft particle suspensions, enables the study of the collective behaviour of the order of 10 6 cells in suspension. After demonstrating the viscosity measurement in Kolmogorov flow, we focus on the statistical analysis of the cell orientation and rotation in Couette flow. We quantify the average inclination with respect to the flow and the nematic order as a function of shear rate and haematocrit. We further rec

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Plunkett, Robert J., Richard J. Weber, and Edward H. Oldfield. "Stereotaxic implantation of dispersed cell suspensions into brain." Journal of Neurosurgery 69, no. 2 (1988): 228–33. http://dx.doi.org/10.3171/jns.1988.69.2.0228.

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✓ The application of several recent advances in cell biology, brain implantation, and cell-mediated tumor immunotherapy requires successful and reproducible placement of viable cell suspensions into brain. Stereotaxic implantation is being used to inject cytotoxic lymphocytes into gliomas and to replace dopaminergic cells in parkinsonian models. Systematic assessment of the factors that influence success in implantation of cell suspensions into solid tissues is needed. A model was developed for investigation of stereotaxic implantation using radiolabeled rat lymphokine-activated killer (LAK) c

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Attree, S. M., D. I. Dunstan, and L. C. Fowke. "Initiation of embryogenic callus and suspension cultures, and improved embryo regeneration from protoplasts, of white spruce (Picea glauca)." Canadian Journal of Botany 67, no. 6 (1989): 1790–95. http://dx.doi.org/10.1139/b89-227.

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Rapid and high frequency somatic embryo regeneration from protoplasts isolated from 10 embryogenic cell lines of white spruce (Picea glauca) is reported. Embryogenic callus was initiated from immature zygotic embryos as source material for protoplast isolation. Individual cell lines exhibited different capabilities for sustained growth. Protoplast plating efficiencies depended on the concentrations of macroelements included in the medium. Using a medium with reduced salts, individual protoplasts developed directly into embryos with no disorganized growth period. Protoplasts from newly establis

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Salih, Shifa M., and Rasha F. Al-Jirjees. "Multi-drops and Cross-sections, Efficient Methods for Establishing Cell Suspension Culture of Cuminum cyminum L. and Plant Regeneration." Basrah Journal of Agricultural Sciences 36, no. 2 (2023): 47–58. http://dx.doi.org/10.37077/25200860.2023.36.2.04.

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The current study aims of the current study is to establish cell suspension cultures of the medicinal plant, cumin Cuminum cyminum L. by the application of multiple drops and cross-section techniques. These methods were used in cultivating cell suspensions and in vitro plant regeneration. Leaf, stem, hypocotyl and root explants were cultured in Murashige and Skoog (MS) medium containing different concentrations (0.05, 0.5 and 1.0 mg.l-1) of naphthalene acetic acid (NAA) and (0.05, 0.1, 1.0 and 2.0 mg.l-1) benzyl adenine BA for callus production. The results indicated the high response of cumin

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Pham, Tram Thi My, and Tien Thi Thuy Le. "STUDYING ON GROWTH OF STRAWBERRY (FRAGARIA ANANASSA L.) CELL SUSPENSION CULTURES FOR ANTHOCYANIN PRODUCTION." Science and Technology Development Journal 15, no. 2 (2012): 69–77. http://dx.doi.org/10.32508/stdj.v15i2.1802.

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Cell suspension cultures were initated from calli derived from in vitro strawberry leaves on MS medium (Murashige and Skoog, 1962) supplemented with 30 g/l sucrose, 1.0 mg/l 2,4-D and 0.3 mg/l kinetin. There were many factors affected on cell suspension cultures growth (it was found that …). Cell suspension cultures grew better on MS medium with 30 g/l sucrose. 1 g (fresh weight) of cells in 20 ml of medium was the best initial inoculum cell density for cell suspension cultures to grow. A shaking speed of 100 rpm on rotary shaker was suitable for the cells. The growth of cell suspension in dar

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Oman, Srecko F., M. Filomena Camões, Kipton J. Powell, Raj Rajagopalan, and Petra Spitzer. "Guidelines for potentiometric measurements in suspensions Part A. The suspension effect (IUPAC Technical Report)." Pure and Applied Chemistry 79, no. 1 (2007): 67–79. http://dx.doi.org/10.1351/pac200779010067.

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An explanation of the origin and interpretation of the suspension effect (SE) is presented in accordance with "pH Measurement: IUPAC Recommendations 2002" [Pure Appl. Chem.74, 2169 (2002)]. It is based on an analysis of detailed schemes of suspension potentiometric cells and confirmed with experimental results. Historically, the term "suspension effect" evolved during attempts to determine electrochemically the thermodynamically defined activity of H+ (aq) in suspensions. The experimental SE arises also in determining other pIon values, analogous to pH values.The SE relates to the observation

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Obeid, Faisal M. "Effect of Autologous Skin Cell Suspensions Versus Standard Treatment on Re-Epithelialization in Burn Injuries: A Meta-Analysis of RCTs." Medicina 61, no. 3 (2025): 529. https://doi.org/10.3390/medicina61030529.

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Background and Objectives: Burn injuries, particularly partial-thickness burns, often require advanced therapies to improve re-epithelialization and scar quality. This study aims to evaluate the efficacy of autologous skin cell suspensions, such as Recell, compared to standard treatments in promoting faster and better-quality skin healing. Our goal is to provide evidence-based conclusions on the effectiveness of these regenerative approaches in burn treatment. Materials and Methods: During our comprehensive investigation, we systematically examined several databases for the period to November

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Wang, Qinyu, Wen Sun, Jianhua Wang, Bin You, and Lingling Sun. "A microfluidic integrated microliter millimeter wave biosensor based on a cell interception structure." Journal of Physics: Conference Series 2809, no. 1 (2024): 012020. http://dx.doi.org/10.1088/1742-6596/2809/1/012020.

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Abstract The millimeter wave frequency band has the characteristics of high resolution, high sensitivity, label free, non-invasive [1], etc. Therefore, this detection technology has broad application prospects in the detection of microliter biological liquid samples. This article is based on the design of coplanar waveguides. A stepped interception structure is designed at the center of the microfluidic path of millimeter wave sensors to intercept and capture tumor cells entering the microfluidic path during fluid injection. This structure is used to improve the accuracy of detection by contro

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Križaj, Dejan, and Borut Pečar. "Analysis of impedance measurements of a suspension of microcapsules using a variable length impedance measurement cell." Journal of Electrical Bioimpedance 3, no. 1 (2019): 42–50. http://dx.doi.org/10.5617/jeb.215.

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Abstract Electrical impedance measurements of a suspension have to take into account the double layer impedance that results from a very thin charged layer formed at the electrode-electrolyte interface. A dedicated measuring cell that enables variation of the distance between the electrodes was developed to investigate the electrical properties of suspensions using two-electrode impedance measurements. By varying the distance between the electrodes it is possible to separate the double layer and the suspension impedance from the measured data. Electrical ‘lumped’ models have been developed fro

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Shishkov, A. V., V. Yu Kulakov, and D. A. Lozovoy. "REPRODUCTION OF THE RABIES VIRUS OF “RV-97” STRAIN IN THE SUSPENSION CULTURE OF VNK-21 / SUSP / ARRIAH AND VNK -21 / 2-17 CELLS." Vestnik of Ulyanovsk state agricultural academy 230 (September 25, 2021): 156–63. http://dx.doi.org/10.18286/1816-4501-2021-3-156-163.

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The study of new suspension cell lines for rabies virus cultivation is a topical area of biotechnology. The article presents results of specification of sensitivity of BNK-21 / SUSP / ARRIAH subline to the rabies virus of “RV-97” strain. A comparison of two highly potential suspension cell cultures of VNK 21 for production of viral material with a high titer of infectious activity is shown [1]. The estimation of the titers of the rabies virus of “RV-97” strain (lg’T, CCID 50 / cm3) was carried out, the titers were established after 48 hours of cultivation in the suspension subline of VNK -21 c

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