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Journal articles on the topic 'Cellobiose Utilization'

Author: Grafiati

Published: 4 June 2021

Last updated: 6 September 2023

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1

Matheron, Christelle, Anne-Marie Delort, Geneviève Gaudet, and Evelyne Forano. "Simultaneous but differential metabolism of glucose and cellobiose inFibrobacter succinogenescells, studied by in vivo13C-NMR." Canadian Journal of Microbiology 42, no. 11 (1996): 1091–99. http://dx.doi.org/10.1139/m96-140.

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Kinetics of [1-13C]glucose utilization were monitored by in vivo NMR spectroscopy on resting cells of Fibrobacter succinogenes, in the presence of 32 mM [1-13C]glucose, 32 mM [1-13C]glucose and 64 mM unlabelled glucose, or 32 mM [1-13C]glucose and 32 mM unlabelled cellobiose. A similar production of acetate and succinate and a similar storage of glycogen were observed whatever the exogenous substrate. The presence of cellobiose or that of an equivalent amount of glucose did not reduce [1-13C]glucose incorporation to the same extent. Glucose seemed preferentially used for glycogen storage and e

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2

Adin, Dawn M., Karen L. Visick та Eric V. Stabb. "Identification of a Cellobiose Utilization Gene Cluster with Cryptic β-Galactosidase Activity in Vibrio fischeri". Applied and Environmental Microbiology 74, № 13 (2008): 4059–69. http://dx.doi.org/10.1128/aem.00190-08.

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ABSTRACT Cellobiose utilization is a variable trait that is often used to differentiate members of the family Vibrionaceae. We investigated how Vibrio fischeri ES114 utilizes cellobiose and found a cluster of genes required for growth on this β-1,4-linked glucose disaccharide. This cluster includes genes annotated as a phosphotransferase system II (celA, celB, and celC), a glucokinase (celK), and a glucosidase (celG). Directly downstream of celCBGKA is celI, which encodes a LacI family regulator that represses cel transcription in the absence of cellobiose. When the celCBGKAI gene cluster was

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3

Oh, Eun Joong, Jeffrey M. Skerker, Soo Rin Kim, et al. "Gene Amplification on Demand Accelerates Cellobiose Utilization in Engineered Saccharomyces cerevisiae." Applied and Environmental Microbiology 82, no. 12 (2016): 3631–39. http://dx.doi.org/10.1128/aem.00410-16.

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ABSTRACTEfficient microbial utilization of cellulosic sugars is essential for the economic production of biofuels and chemicals. Although the yeastSaccharomyces cerevisiaeis a robust microbial platform widely used in ethanol plants using sugar cane and corn starch in large-scale operations, glucose repression is one of the significant barriers to the efficient fermentation of cellulosic sugar mixtures. A recent study demonstrated that intracellular utilization of cellobiose by engineered yeast expressing a cellobiose transporter (encoded bycdt-1) and an intracellular β-glucosidase (encoded byg

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4

Shulami, Smadar, Arie Zehavi, Valery Belakhov та ін. "Cross-utilization of β-galactosides and cellobiose in Geobacillus stearothermophilus". Journal of Biological Chemistry 295, № 31 (2020): 10766–80. http://dx.doi.org/10.1074/jbc.ra120.014029.

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Strains of the Gram-positive, thermophilic bacterium Geobacillus stearothermophilus possess elaborate systems for the utilization of hemicellulolytic polysaccharides, including xylan, arabinan, and galactan. These systems have been studied extensively in strains T-1 and T-6, representing microbial models for the utilization of soil polysaccharides, and many of their components have been characterized both biochemically and structurally. Here, we characterized routes by which G. stearothermophilus utilizes mono- and disaccharides such as galactose, cellobiose, lactose, and galactosyl-glycerol.

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5

Parker, L. L., and B. G. Hall. "A fourth Escherichia coli gene system with the potential to evolve beta-glucoside utilization." Genetics 119, no. 3 (1988): 485–90. http://dx.doi.org/10.1093/genetics/119.3.485.

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Abstract Escherichia coli K12 is being used to study the potential for adaptive evolution that is present in the genome of a single organism. Wild-type E. coli K12 do not utilize any of the beta-glucoside sugars arbutin, salicin or cellobiose. It has been shown that mutations at three cryptic loci allow utilization of these sugars. Mutations in the bgl operon allow inducible growth on arbutin and salicin while cel mutations allow constitutive utilization of cellobiose as well as arbutin and salicin. Mutations in a third cryptic locus, arbT, allow the transport of arbutin. A salicin+ arbutin+ c

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6

Fosses, Aurélie, Nathalie Franche, Goetz Parsiegla, et al. "Role of the Solute-Binding Protein CuaD in the Signaling and Regulating Pathway of Cellobiose and Cellulose Utilization in Ruminiclostridium cellulolyticum." Microorganisms 11, no. 7 (2023): 1732. http://dx.doi.org/10.3390/microorganisms11071732.

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In Ruminiclostridium cellulolyticum, cellobiose is imported by the CuaABC ATP-binding cassette transporter containing the solute-binding protein (SBP) CuaA and is further degraded in the cytosol by the cellobiose phosphorylase CbpA. The genes encoding these proteins have been shown to be essential for cellobiose and cellulose utilization. Here, we show that a second SBP (CuaD), whose gene is adjacent to two genes encoding a putative two-component regulation system (CuaSR), forms a three-component system with CuaS and CuaR. Studies of mutant and recombinant strains of R. cellulolyticum have ind

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7

Hetzler, Stephan, and Alexander Steinbüchel. "Establishment of Cellobiose Utilization for Lipid Production in Rhodococcus opacus PD630." Applied and Environmental Microbiology 79, no. 9 (2013): 3122–25. http://dx.doi.org/10.1128/aem.03678-12.

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ABSTRACTRhodococcus opacusPD630, which is known for its ability to accumulate large amounts of triacylglycerols (TAG), was metabolically engineered, and a cellobiose utilization pathway was introduced. Activities of β-glucosidases were determined, and recombinant strains accumulated fatty acids up to 39.5 ± 5.7% (wt/wt) of cell dry mass from cellobiose.

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8

Bernier, Rene, and Fred Stutzenberger. "Preferential Utilization of Cellobiose by Thermomonospora curvata." Applied and Environmental Microbiology 53, no. 8 (1987): 1743–47. http://dx.doi.org/10.1128/aem.53.8.1743-1747.1987.

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9

Vinuselvi, Parisutham, and Sung Kuk Lee. "Engineering Escherichia coli for efficient cellobiose utilization." Applied Microbiology and Biotechnology 92, no. 1 (2011): 125–32. http://dx.doi.org/10.1007/s00253-011-3434-9.

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10

Kricker, Maja, and Barry G. Hall. "Biochemical Genetics of the Cryptic Gene System for Cellobiose Utilization in Escherichia coli K12." Genetics 115, no. 3 (1987): 419–29. http://dx.doi.org/10.1093/genetics/115.3.419.

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ABSTRACT The cellobiose catabolic system of Escherichia coli K12 is being used to study the role of cryptic genes in microbial evolution. Wild-type E. coli K12 do not utilize the β-glucoside sugars, arbutin, salicin and cellobiose. A Cel+ (cellobiose utilizing) mutant which grows on cellobiose, arbutin, and salicin was isolated previously from wild-type E. coli K12. Biochemical assays indicate that a cel structural gene (celT) specifies a single transport protein that is a β-glucoside specific enzyme of the phosphoenolpyruvate-dependent phosphotransferase system. The transport protein phosphor

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11

Murata, Masayuki, Sornsiri Pattanakittivorakul, Toshiro Manabe, Savitree Limtong, and Mamoru Yamada. "Mutants with Enhanced Cellobiose-Fermenting Ability from Thermotolerant Kluyveromyces marxianus DMKU 3-1042, Which Are Beneficial for Fermentation with Cellulosic Biomass." Fuels 3, no. 2 (2022): 232–44. http://dx.doi.org/10.3390/fuels3020015.

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Several cellulose-hydrolysis enzymes are required for eco-friendly utilization of cellulose as renewable biomass, and it would therefore be beneficial if fermenting microbes can provide such enzymes without genetic engineering. Thermotolerant and multisugar-fermenting Kluyveromyces marxianus is one of the promising yeasts for high-temperature fermentation and has genes for putative oligosaccharide-degradation enzymes. Mutants obtained after multiple mutagenesis showed significantly higher activity than that of the parental strain for cellobiose fermentation. The efficient strains were found to

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12

Adsul, Mukund, Jayant Khire, Kulbhushan Bastawde, and Digambar Gokhale. "Production of Lactic Acid from Cellobiose and Cellotriose by Lactobacillus delbrueckii Mutant Uc-3." Applied and Environmental Microbiology 73, no. 15 (2007): 5055–57. http://dx.doi.org/10.1128/aem.00774-07.

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ABSTRACT Lactobacillus delbrueckii mutant Uc-3 utilizes both cellobiose and cellotriose efficiently, converting it into L(+) lactic acid. The enzyme activities of cellobiose and cellotriose utilization were determined for cell extracts, whole cells, and disrupted cells. Aryl-β-glucosidase activity was detected only for whole cells and disrupted cells, suggesting that these activities are cell bound. The mutant produced 90 g/liter of lactic acid from 100 g/liter of cellobiose with 2.25 g/liter/h productivity.

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13

van Zanten, Gabriella C., Nadja Sparding, Avishek Majumder, Sampo J. Lahtinen, Birte Svensson та Susanne Jacobsen. "The Differential Proteome of the ProbioticLactobacillus acidophilusNCFM Grown on the Potential Prebiotic Cellobiose Shows Upregulation of Twoβ-Glycoside Hydrolases". BioMed Research International 2015 (2015): 1–9. http://dx.doi.org/10.1155/2015/347216.

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Probiotics, prebiotics, and combinations thereof, that is, synbiotics, are known to exert beneficial health effects in humans; however interactions between pro- and prebiotics remain poorly understood at the molecular level. The present study describes changes in abundance of different proteins of the probiotic bacteriumLactobacillus acidophilusNCFM (NCFM) when grown on the potential prebiotic cellobiose as compared to glucose. Cytosolic cell extract proteomes after harvest at late exponential phase of NCFM grown on cellobiose or glucose were analyzed by two dimensional difference gel electrop

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14

Hall, Barry G., and Paul W. Betts. "Cryptic Genes for Cellobiose Utilization in Natural Isolates of Escherichia coli." Genetics 115, no. 3 (1987): 431–39. http://dx.doi.org/10.1093/genetics/115.3.431.

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ABSTRACT The ECOR collection of natural Escherichia coli isolates was screened to determine the proportion of strains that carried functional, cryptic and nonfunctional genes for utilization of the three β-glucoside sugars, arbutin, salicin and cellobiose. None of the 71 natural isolates utilized any of the β-glucosides. Each strain was subjected to selection for utilization of each of the sugars. Only five of the isolates were incapable of yielding spontaneous β-glucoside-utilizing mutants. Forty-five strains yielded cellobiose+ mutants, 62 yielded arbutin+ mutants, and 58 strains yielded sal

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15

Shafeeq, Sulman, Tomas G. Kloosterman, and Oscar P. Kuipers. "CelR-mediated activation of the cellobiose-utilization gene cluster in Streptococcus pneumoniae." Microbiology 157, no. 10 (2011): 2854–61. http://dx.doi.org/10.1099/mic.0.051359-0.

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The human pathogen Streptococcus pneumoniae harbours many genes encoding phosphotransferase systems and sugar ABC (ATP-binding cassette) transporters, including systems for the utilization of the β-glucoside sugar cellobiose. In this study, we show that the transcriptional regulator CelR, which has previously been found to be important for pneumococcal virulence, activates the expression of the cellobiose-utilization gene cluster (cel locus) of S. pneumoniae. Expression directed by the two promoters present in the cel locus was increased in the presence of cellobiose as sole carbon source in t

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16

Freer, S. N. "Utilization of glucose and cellobiose by Candida molischiana." Canadian Journal of Microbiology 41, no. 2 (1995): 177–85. http://dx.doi.org/10.1139/m95-024.

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Some of the factors that influence the biosynthesis of the Candida molischiana β-glucosidase were investigated. The yeast produced maximal enzyme activity when grown at 28 °C in a carbohydrate-containing complex medium (YM) in which the initial pH was adjusted to 6.0. The enzyme appeared to be produced constitutively, as activity was detected when either ethanol, glycerol, xylose, glucitol, mannitol, maltose, trehalose, cellobiose, cellodextrins, or soluble starch was used as the carbohydrate source. The presence of either glucose, mannose, or fructose (> 25 g/L) repressed β-glucosidase exp

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17

Yernool, Dinesh A., James K. McCarthy, Douglas E. Eveleigh та Jin-Duck Bok. "Cloning and Characterization of the Glucooligosaccharide Catabolic Pathway β-Glucan Glucohydrolase and Cellobiose Phosphorylase in the Marine HyperthermophileThermotoga neapolitana". Journal of Bacteriology 182, № 18 (2000): 5172–79. http://dx.doi.org/10.1128/jb.182.18.5172-5179.2000.

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ABSTRACT Characterization in Thermotoga neapolitana of a catabolic gene cluster encoding two glycosyl hydrolases, 1,4-β-d-glucan glucohydrolase (GghA) and cellobiose phosphorylase (CbpA), and the apparent absence of a cellobiohydrolase (Cbh) suggest a nonconventional pathway for glucan utilization inThermotogales. GghA purified from T. neapolitana is a 52.5-kDa family 1 glycosyl hydrolase with optimal activity at pH 6.5 and 95°C. GghA releases glucose from soluble glucooligomers, with a preference for longer oligomers:k cat/Km values are 155.2, 76.0, and 9.9 mM−1 s−1 for cellotetraose, cellotr

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Freer, Shelby N., та Christopher D. Skory. "Production of β-glucosidase and diauxic usage of sugar mixtures byCandida molischiana". Canadian Journal of Microbiology 42, № 5 (1996): 431–36. http://dx.doi.org/10.1139/m96-059.

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The fermentation of cellobiose is a rare trait among yeasts. Of the 308 yeast species that utilize cellobiose aerobically, only 12 species ferment it, and only 2 species, Candida molischiana and Candida wickerhamii, also ferment cellodextrins. Candida molischiana produced β-glucosidase activity on all carbon sources tested, except glucose, mannose, and fructose. When these sugars were added to cultures growing on cellobiose, the synthesis of β-glucosidase ceased. However, the total amount of enzyme activity remained constant, indicating that the C. molischiana β-glucosidase is catabolite repre

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19

Tilly, Kit, Abdallah F. Elias, Jennifer Errett, et al. "Genetics and Regulation of Chitobiose Utilization inBorrelia burgdorferi." Journal of Bacteriology 183, no. 19 (2001): 5544–53. http://dx.doi.org/10.1128/jb.183.19.5544-5553.2001.

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ABSTRACT Borrelia burgdorferi spends a significant proportion of its life cycle within an ixodid tick, which has a cuticle containing chitin, a polymer of N-acetylglucosamine (GlcNAc). TheB. burgdorferi celA, celB, andcelC genes encode products homologous to transporters for cellobiose and chitobiose (the dimer subunit of chitin) in other bacteria, which could be useful for bacterial nutrient acquisition during growth within ticks. We found that chitobiose efficiently substituted for GlcNAc during bacterial growth in culture medium. We inactivated the celB gene, which encodes the putative memb

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20

Cao, Yingnan, Juan Wang, Qunhui Wang та ін. "Effect of β-glycosidase supplementation on vinasse saccharification and L-lactic acid fermentation". BioResources 14, № 1 (2019): 1379–89. http://dx.doi.org/10.15376/biores.14.1.1379-1389.

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Efficient pretreatment and enzymatic hydrolysis is critical to achieve effective utilization of lignocellulosic biomass. In this study, the cellulase composition for lignocellulosic biomass hydrolysis was strategically optimized to improve the efficiency of vinasse saccharification and thus enhance L-lactic acid production. The results showed that the supplementation of β-glycosidase (BG) increased sugar production, and the glucose concentration exceeded cellobiose concentration after 48 h of hydrolysis. These results suggested that the addition of BG aided the hydrolysis of cellobiose and red

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Ryu, Seunghyun, Julie Hipp, and Cong T. Trinh. "Activating and Elucidating Metabolism of Complex Sugars in Yarrowia lipolytica." Applied and Environmental Microbiology 82, no. 4 (2015): 1334–45. http://dx.doi.org/10.1128/aem.03582-15.

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ABSTRACTThe oleaginous yeastYarrowia lipolyticais an industrially important host for production of organic acids, oleochemicals, lipids, and proteins with broad biotechnological applications. Albeit known for decades, the unique native metabolism ofY. lipolyticafor using complex fermentable sugars, which are abundant in lignocellulosic biomass, is poorly understood. In this study, we activated and elucidated the native sugar metabolism inY. lipolyticafor cell growth on xylose and cellobiose as well as their mixtures with glucose through comprehensive metabolic and transcriptomic analyses. We i

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Thurston, B., K. A. Dawson, and H. J. Strobel. "Cellobiose versus glucose utilization by the ruminal bacterium Ruminococcus albus." Applied and Environmental Microbiology 59, no. 8 (1993): 2631–37. http://dx.doi.org/10.1128/aem.59.8.2631-2637.1993.

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23

Jame, R., V. Zelená, B. Lakatoš, and Ľ. Varečka. "Carbon source utilization and hydrogen production by isolated anaerobic bacteria." Acta Chimica Slovaca 9, no. 1 (2016): 62–67. http://dx.doi.org/10.1515/acs-2016-0011.

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Abstract Five bacterial isolates were tested for their ability to generate hydrogen during anaerobic fermentation with various carbon sources. One isolate from sheep rumen was identified as Escherichia coli and four isolates belonged to Clostridium spp. Glucose, arabinose, ribose, xylose, lactose and cellobiose were used as carbon sources. Results showed that all bacterial strains could utilize these compounds, although the utilization of pentoses diminished growth yield. The excretion of monocarboxylic acids (acetate, propionate, formiate, butyrate) into medium was changed after replacing glu

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Shiwa, Yuh, Haruko Fujiwara, Mao Numaguchi, et al. "Transcriptome profile of carbon catabolite repression in an efficient l-(+)-lactic acid-producing bacterium Enterococcus mundtii QU25 grown in media with combinations of cellobiose, xylose, and glucose." PLOS ONE 15, no. 11 (2020): e0242070. http://dx.doi.org/10.1371/journal.pone.0242070.

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Enterococcus mundtii QU25, a non-dairy lactic acid bacterium of the phylum Firmicutes, is capable of simultaneously fermenting cellobiose and xylose, and is described as a promising strain for the industrial production of optically pure l-lactic acid (≥ 99.9%) via homo-fermentation of lignocellulosic hydrolysates. Generally, Firmicutes bacteria show preferential consumption of sugar (usually glucose), termed carbon catabolite repression (CCR), while hampering the catabolism of other sugars. In our previous study, QU25 exhibited apparent CCR in a glucose-xylose mixture phenotypically, and trans

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Bhat, M. K. "Potential application of cellulase and hemicellulase assay techniques for assessing the forage quality and performance of rumen micro-organisms." BSAP Occasional Publication 22 (1998): 290–93. http://dx.doi.org/10.1017/s0263967x00032900.

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Cellulose and hemicellulose are the major structural polysaccharides of plant cell wall. The efficient utilization of these polysaccharides by ruminants is often restricted by the presence of lignin. Cellulose and hemicellulose are hydrolysed by a group of enzymes called cellulases and hemicellulases. The present paper describes the cellulase and hemicellulase assay methods and their potential applications.Carboxymethyl (CM)-cellulose, Avicel, cellobiose, xylobiose, p-nitrophenyl-p β-D-glucoside (pNPG), p-nitrophenyl-β-D-cellobioside (pNPC), p-nitrophenyl-β-D-xyloside (pNPX) and p-nitrophenyl-

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Kotrba, Pavel, Masayuki Inui та Hideaki Yukawa. "A single V317A or V317M substitution in Enzyme II of a newly identified β-glucoside phosphotransferase and utilization system of Corynebacterium glutamicum R extends its specificity towards cellobiose". Microbiology 149, № 6 (2003): 1569–80. http://dx.doi.org/10.1099/mic.0.26053-0.

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A catabolic system involved in the utilization of β-glucosides in Corynebacterium glutamicum R and its spontaneous mutant variants allowing uptake of cellobiose were investigated. The system comprises a β-glucoside-specific Enzyme IIBCA component (gene bglF) of the phosphotransferase system (PTS), a phospho-β-glucosidase (bglA) and an antiterminator protein (bglG) from the BglG/SacY family of transcription regulators. The results suggest that transcription antitermination is involved in control of induction and carbon catabolite repression of bgl genes, which presumably form an operon. Functio

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Hall, B. G., and W. Faunce. "Functional genes for cellobiose utilization in natural isolates of Escherichia coli." Journal of Bacteriology 169, no. 6 (1987): 2713–17. http://dx.doi.org/10.1128/jb.169.6.2713-2717.1987.

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Shin, Hyun-Dong, Jianrong Wu, and Rachel Chen. "Comparative engineering of Escherichia coli for cellobiose utilization: Hydrolysis versus phosphorolysis." Metabolic Engineering 24 (July 2014): 9–17. http://dx.doi.org/10.1016/j.ymben.2014.04.002.

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Vinuselvi, Parisutham, and Sung Kuk Lee. "Engineered Escherichia coli capable of co-utilization of cellobiose and xylose." Enzyme and Microbial Technology 50, no. 1 (2012): 1–4. http://dx.doi.org/10.1016/j.enzmictec.2011.10.001.

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Marasco, R., C. T. Lago та M. De Felice. "Utilization of cellobiose and other β-D-glucosides in Agrobacterium tumefaciens". Research in Microbiology 146, № 6 (1995): 485–92. http://dx.doi.org/10.1016/0923-2508(96)80294-4.

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Ji, Xiaofei, Ying Wang, Cong Zhang, Xinfeng Bai, Weican Zhang, and Xuemei Lu. "Novel Outer Membrane Protein Involved in Cellulose and Cellooligosaccharide Degradation by Cytophaga hutchinsonii." Applied and Environmental Microbiology 80, no. 15 (2014): 4511–18. http://dx.doi.org/10.1128/aem.00687-14.

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ABSTRACTCytophaga hutchinsoniiis an aerobic cellulolytic soil bacterium which was reported to use a novel contact-dependent strategy to degrade cellulose. It was speculated that cellooligosaccharides were transported into the periplasm for further digestion. In this study, we reported that most of the endoglucanase and β-glucosidase activity was distributed on the cell surface ofC. hutchinsonii. Cellobiose and part of the cellulose could be hydrolyzed to glucose on the cell surface. However, the cell surface cellulolytic enzymes were not sufficient for cellulose degradation byC. hutchinsonii.

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Faure, Denis, Jos Desair, Veerle Keijers та ін. "Growth of Azospirillum irakense KBC1 on the Aryl β-Glucoside Salicin Requires either salA or salB". Journal of Bacteriology 181, № 10 (1999): 3003–9. http://dx.doi.org/10.1128/jb.181.10.3003-3009.1999.

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ABSTRACT The rhizosphere nitrogen-fixing bacteriumAzospirillum irakense KBC1 is able to grow on pectin and β-glucosides such as cellobiose, arbutin, and salicin. Two adjacent genes, salA and salB, conferring β-glucosidase activity to Escherichia coli, have been identified in a cosmid library of A. irakense DNA. The SalA and SalB enzymes preferentially hydrolyzed aryl β-glucosides. A Δ(salA-salB) A. irakense mutant was not able to grow on salicin but could still utilize arbutin, cellobiose, and glucose for growth. This mutant could be complemented by either salA or salB, suggesting functional r

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Julliand, Veronique, Albane de Vaux, Liliane Millet, and Gerard Fonty. "Identification of Ruminococcus flavefaciens as the Predominant Cellulolytic Bacterial Species of the Equine Cecum." Applied and Environmental Microbiology 65, no. 8 (1999): 3738–41. http://dx.doi.org/10.1128/aem.65.8.3738-3741.1999.

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ABSTRACT Detection and quantification of cellulolytic bacteria with oligonucleotide probes showed that Ruminococcus flavefaciens was the predominant species in the pony and donkey cecum. Fibrobacter succinogenes and Ruminococcus albus were present at low levels. Four isolates, morphologically resembling R. flavefaciens, differed from ruminal strains by their carbohydrate utilization and their end products of cellobiose fermentation.

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Marushima, Kazuya, Yasuo Ohnishi, and Sueharu Horinouchi. "CebR as a Master Regulator for Cellulose/Cellooligosaccharide Catabolism Affects Morphological Development in Streptomyces griseus." Journal of Bacteriology 191, no. 19 (2009): 5930–40. http://dx.doi.org/10.1128/jb.00703-09.

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ABSTRACT Streptomyces griseus mutants exhibiting deficient glucose repression of β-galactosidase activity on lactose-containing minimal medium supplemented with a high concentration of glucose were isolated. One of these mutants had a 12-bp deletion in cebR, which encodes a LacI/GalR family regulator. Disruption of cebR in the wild-type strain caused the same phenotype as the mutant, indicating that CebR is required for glucose repression of β-galactosidase activity. Recombinant CebR protein bound to a 14-bp inverted-repeat sequence (designated the CebR box) present in the promoter regions of

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Parker, L. L., and B. G. Hall. "Mechanisms of activation of the cryptic cel operon of Escherichia coli K12." Genetics 124, no. 3 (1990): 473–82. http://dx.doi.org/10.1093/genetics/124.3.473.

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Abstract The cel (cellobiose utilization) operon of Escherichia coli K12 is not expressed in the wild-type organism. However, mutants that can express the operon and thereby utilize the beta-glucoside sugars cellobiose, arbutin and salicin are easily isolated. Two kinds of mutations are capable of activating the operon. The first involves mutations that allow the repressor to recognize the substrates cellobiose, arbutin and salicin as inducers. We have identified the sequence changes in five different active alleles and found those differences to be single base pair changes at one of two lysin

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Pokusaeva, Karina, Mary O'Connell-Motherway, Aldert Zomer, John MacSharry, Gerald F. Fitzgerald, and Douwe van Sinderen. "Cellodextrin Utilization byBifidobacterium breveUCC2003." Applied and Environmental Microbiology 77, no. 5 (2011): 1681–90. http://dx.doi.org/10.1128/aem.01786-10.

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ABSTRACTCellodextrins, the incomplete hydrolysis products from insoluble cellulose, are accessible as a carbon source to certain members of the human gut microbiota, such asBifidobacterium breveUCC2003. Transcription of thecldEFGCgene cluster ofB. breveUCC2003 was shown to be induced upon growth on cellodextrins, implicating this cluster in the metabolism of these sugars. Phenotypic analysis of aB. breveUCC2003::cldEinsertion mutant confirmed that thecldgene cluster is exclusively required for cellodextrin utilization by this commensal. Moreover, our results suggest that transcription of thecl

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Parisutham, Vinuselvi, Sang-Kyu Jung, Dougu Nam, and Sung Kuk Lee. "Transcriptome-driven synthetic re-modeling of Escherichia coli to enhance cellobiose utilization." Chemical Engineering Science 103 (November 2013): 50–57. http://dx.doi.org/10.1016/j.ces.2012.08.006.

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Qu, Chunyun, Yang Zhang, Kaiqun Dai, Hongxin Fu, and Jufang Wang. "Metabolic engineering of Thermoanaerobacterium aotearoense SCUT27 for glucose and cellobiose co-utilization by identification and overexpression of the endogenous cellobiose operon." Biochemical Engineering Journal 167 (March 2021): 107922. http://dx.doi.org/10.1016/j.bej.2020.107922.

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Yoav, Shahar, Johanna Stern, Orly Salama-Alber та ін. "Directed Evolution of Clostridium thermocellum β-Glucosidase A Towards Enhanced Thermostability". International Journal of Molecular Sciences 20, № 19 (2019): 4701. http://dx.doi.org/10.3390/ijms20194701.

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β-Glucosidases are key enzymes in the process of cellulose utilization. It is the last enzyme in the cellulose hydrolysis chain, which converts cellobiose to glucose. Since cellobiose is known to have a feedback inhibitory effect on a variety of cellulases, β-glucosidase can prevent this inhibition by hydrolyzing cellobiose to non-inhibitory glucose. While the optimal temperature of the Clostridium thermocellum cellulosome is 70 °C, C. thermocellum β-glucosidase A is almost inactive at such high temperatures. Thus, in the current study, a random mutagenesis directed evolutionary approach was c

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Chassard,, Christophe, Eve Delmas,, Céline Robert,, Paul A. Lawson, and Annick Bernalier-Donadille. "Ruminococcus champanellensis sp. nov., a cellulose-degrading bacterium from human gut microbiota." International Journal of Systematic and Evolutionary Microbiology 62, no. 1 (2012): 138–43. http://dx.doi.org/10.1099/ijs.0.027375-0.

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A strictly anaerobic, cellulolytic strain, designated 18P13T, was isolated from a human faecal sample. Cells were Gram-positive non-motile cocci. Strain 18P13T was able to degrade microcrystalline cellulose but the utilization of soluble sugars was restricted to cellobiose. Acetate and succinate were the major end products of cellulose and cellobiose fermentation. 16S rRNA gene sequence analysis revealed that the isolate belonged to the genus Ruminococcus of the family Ruminococcaceae. The closest phylogenetic relative was the ruminal cellulolytic strain Ruminococcus flavefaciens ATCC 19208T (

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Brehm, Klaus, María-Teresa Ripio, Jürgen Kreft та José-Antonio Vázquez-Boland. "The bvr Locus of Listeria monocytogenes Mediates Virulence Gene Repression by β-Glucosides". Journal of Bacteriology 181, № 16 (1999): 5024–32. http://dx.doi.org/10.1128/jb.181.16.5024-5032.1999.

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ABSTRACT The β-glucoside cellobiose has been reported to specifically repress the PrfA-dependent virulence genes hly andplcA in Listeria monocytogenes NCTC 7973. This led to the hypothesis that β-glucosides, sugars of plant origin, may act as signal molecules, preventing the expression of virulence genes if L. monocytogenes is living in its natural habitat (soil). In three other laboratory strains (EGD, L028, and 10403S), however, the effect of cellobiose was not unique, and all fermentable carbohydrates repressed hly. This suggested that the downregulation of virulence genes by β-glucosides i

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Cao, Thanh Nguyen, Philippe Joyet, Francine Moussan Désirée Aké, Eliane Milohanic, and Josef Deutscher. "Studies of the Listeria monocytogenes Cellobiose Transport Components and Their Impact on Virulence Gene Repression." Journal of Molecular Microbiology and Biotechnology 29, no. 1-6 (2019): 10–26. http://dx.doi.org/10.1159/000500090.

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<b><i>Background:</i></b> Many bacteria transport cellobiose via a phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In <i>Listeria monocytogenes</i>, two pairs of soluble PTS components (EIIA<sup>Cel1</sup>/EIIB<sup>Cel1</sup> and EIIA<sup>Cel2</sup>/EIIB<sup>Cel2</sup>) and the permease EIIC<sup>Cel1</sup> were suggested to contribute to cellobiose uptake. Interestingly, utilization of several carbohydrates, including cellobiose, strongly represses virulence gene expression by inhi

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Li, Sijin, Suk-Jin Ha, Hee Jin Kim, et al. "Investigation of the functional role of aldose 1-epimerase in engineered cellobiose utilization." Journal of Biotechnology 168, no. 1 (2013): 1–6. http://dx.doi.org/10.1016/j.jbiotec.2013.08.003.

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Yu, Xiaochen, Yubin Zheng, Xiaochao Xiong, and Shulin Chen. "Co-utilization of glucose, xylose and cellobiose by the oleaginous yeast Cryptococcus curvatus." Biomass and Bioenergy 71 (December 2014): 340–49. http://dx.doi.org/10.1016/j.biombioe.2014.09.023.

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Guo, Zhong-peng, Liang Zhang, Zhong-yang Ding, Zheng-hua Gu, and Gui-yang Shi. "Development of an industrial ethanol-producing yeast strain for efficient utilization of cellobiose." Enzyme and Microbial Technology 49, no. 1 (2011): 105–12. http://dx.doi.org/10.1016/j.enzmictec.2011.02.008.

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Rutter, Charles, and Rachel Chen. "Improved cellobiose utilization in E. coli by including both hydrolysis and phosphorolysis mechanisms." Biotechnology Letters 36, no. 2 (2013): 301–7. http://dx.doi.org/10.1007/s10529-013-1355-7.

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Long, Tanya M., Yi-Kai Su, Jennifer Headman, Alan Higbee, Laura B. Willis, and Thomas W. Jeffries. "Cofermentation of Glucose, Xylose, and Cellobiose by the Beetle-Associated Yeast Spathaspora passalidarum." Applied and Environmental Microbiology 78, no. 16 (2012): 5492–500. http://dx.doi.org/10.1128/aem.00374-12.

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ABSTRACTFermentation of cellulosic and hemicellulosic sugars from biomass could resolve food-versus-fuel conflicts inherent in the bioconversion of grains. However, the inability to coferment glucose and xylose is a major challenge to the economical use of lignocellulose as a feedstock. Simultaneous cofermentation of glucose, xylose, and cellobiose is problematic for most microbes because glucose represses utilization of the other saccharides. Surprisingly, the ascomycetous, beetle-associated yeastSpathaspora passalidarum, which ferments xylose and cellobiose natively, can also coferment these

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Verma, Subhash Chandra, and Subramony Mahadevan. "ThechbGGene of the Chitobiose (chb) Operon of Escherichia coli Encodes a Chitooligosaccharide Deacetylase." Journal of Bacteriology 194, no. 18 (2012): 4959–71. http://dx.doi.org/10.1128/jb.00533-12.

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ABSTRACTThechboperon ofEscherichia coliis involved in the utilization of the β-glucosides chitobiose and cellobiose. The function ofchbG(ydjC), the sixth open reading frame of the operon that codes for an evolutionarily conserved protein is unknown. We show thatchbGencodes a monodeacetylase that is essential for growth on the acetylated chitooligosaccharides chitobiose and chitotriose but is dispensable for growth on cellobiose and chitosan dimer, the deacetylated form of chitobiose. The predicted active site of the enzyme was validated by demonstrating loss of function upon substitution of it

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Abu Bakar, Nurul Kartini, Zuraidah Zanirun, Suraini Abd-Aziz, Farinazleen Mohd Ghazali, and Mohd Ali Hassan. "Production of fermentable sugars from oil palm empty fruit bunch using crude cellulase cocktails with Trichoderma asperellum UPM1 and Aspergillus fumigatus UPM2 for bioethanol production." BioResources 7, no. 3 (2012): 3627–39. http://dx.doi.org/10.15376/biores.7.3.3627-3639.

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Utilization of oil palm empty fruit bunch (OPEFB) for bioethanol production with crude cellulase cocktails from locally isolated fungi was studied. Enzymatic saccharification of alkaline pretreated OPEFB was done using different cellulase enzyme preparations. Crude cellulase cocktails from Trichoderma asperellum UPM1 and Aspergillus fumigatus UPM2 produced 8.37 g/L reducing sugars with 0.17 g/g yield. Production of bioethanol from OPEFB hydrolysate using Baker’s yeast produced approximately 0.59 g/L ethanol, corresponding to 13.8% of the theoretical yield. High reducing sugars concentration in

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Cabulong, Rhudith B., Angelo B. Bañares, Grace M. Nisola, Won-Keun Lee, and Wook-Jin Chung. "Enhanced glycolic acid yield through xylose and cellobiose utilization by metabolically engineered Escherichia coli." Bioprocess and Biosystems Engineering 44, no. 6 (2021): 1081–91. http://dx.doi.org/10.1007/s00449-020-02502-6.

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