Relevant bibliographies by topics / Cells. Stains and staining (Microscopy)

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers

Academic literature on the topic 'Cells. Stains and staining (Microscopy)'

Author: Grafiati
Published: 4 June 2021
Last updated: 5 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Cells. Stains and staining (Microscopy).'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Cells. Stains and staining (Microscopy)"

1

OPPONG, DAVID, and BIRDELL H. SNUDDEN. "Comparison of Acridine Orange Staining Using Fluorescence Microscopy with Traditional Methods for Microbiological Examination of Selected Dry Food Products." Journal of Food Protection 51, no. 6 (1988): 485–88. http://dx.doi.org/10.4315/0362-028x-51.6.485.

Full text
Abstract:
A comparison was made between the acridine orange stain, gram stain and methylene blue stain for direct microscopic counts (DMC) of microorganisms in gravy mixes, spices, cocoa products and baby foods. Bacteria were detected in 96% (45/47) of the samples stained with acridine orange, 64% (30/47) for the gram stain and 66% (31/47) for the methylene blue stain. In most instances, acridine-orange smears showed higher numbers of bacteria than the traditional stains. The staining quality of the acridine orange was better than the conventional stains with bacteria, yeast cells, and mold hyphae fluorescing very differently from the background. The results indicate that direct staining with acridine orange is better than the traditional methods for estimating bacterial numbers in such foods.
APA, Harvard, Vancouver, ISO, and other styles
2

Smith, Stephen A., Shelley J. Newman, Matthew P. Coleman, and Charles Alex. "Characterization of the histologic appearance of normal gill tissue using special staining techniques." Journal of Veterinary Diagnostic Investigation 30, no. 5 (2018): 688–98. http://dx.doi.org/10.1177/1040638718791819.

Full text
Abstract:
Anatomic pathologists are familiar with stains used in light microscopy to identify cells, storage products, tissue deposits, and pathogens. Assessment of the surrounding tissue with special stains may reveal aspects of interest for the tissue or the species. We illustrate the expected staining characteristics of normal rainbow trout gill tissue with routine hematoxylin and eosin and 18 other histochemical stains.
APA, Harvard, Vancouver, ISO, and other styles
3

Calomeni, E. P., and W. T. Gunning. "A modification of the Humphrey’s stain for epoxy sections: A suitable alternative to toluidine blue for routine section staining." Proceedings, annual meeting, Electron Microscopy Society of America 51 (August 1, 1993): 244–45. http://dx.doi.org/10.1017/s0424820100147065.

Full text
Abstract:
We have abandoned the use of toluidine blue as a routine stain for light microscopy of epoxy embedded tissues. As an alternative, we utilize a three-component stain described by Humphrey and Pittman (1974) with minimal modifications to render the procedure substantially more timely. Numerous publications in the early 60’s through mid 70’s proposed a variety of polychromatic stains for light microscopic evaluation of epoxy sections. Most of these reports cited the difficulties involved in using a reliable method of staining epoxy sections in a timely and non-cumbersome fashion. One of the most successful stains to address these concerns is toluidine blue, first described by Trump el al. in 1961, which is undoubtedly the stain of choice in most laboratories. We have found that the differentiation of stained tissues when using the Humphrey’s stain is superior to that rendered by toluidine blue. This stain differentiates cellular detail from connective tissue in brilliant fashion as most cells will appear in various shades of blue (quite similar to shades produced by toluidine blue) and collagen appears a vivid deep pink to bright red.
APA, Harvard, Vancouver, ISO, and other styles
4

Hong, Suk J., and Richik N. Ghosh. "Effective Cell Identification and Segmentation in Fluorescence Microscopy with New Fluorescent Whole Cell Stains." Microscopy Today 16, no. 1 (2008): 12–15. http://dx.doi.org/10.1017/s1551929500054262.

Full text
Abstract:
High-content screening (HCS) and other quantitative cellular imaging assay methods that use fluorescence microscopy require effective fluorescent labeling and identification of the target cell(s). Fluorescent probes that stain cells as the primary objects are used to identify and count individual cells, as well as to define the region(s) of each cell to which target-specific image analysis is applied. For this purpose, the primary object can be a major component of the cell, such as the nucleus or a large organelle, or the whole cell itself. When the whole cell is the primary object, a high-quality cell stain is needed to delineate the intact cell from bordering cells without also interfering in target-specific detection and analysis. Thermo Scientific Cellomics ® Whole Cell Stains (Thermo Fisher Scientific Inc., Rockford, Ill.), available in blue, green, orange and red, provides image staining and the ability to quantify the whole cell volume in HCS and fluorescence microscopy assays.
APA, Harvard, Vancouver, ISO, and other styles
5

Chen, Y. J., W. F. Hickey, S. G. Mezitis, et al. "Monoclonal antibody 2H1 detects a 60-65 KD membrane polypeptide of the rough endoplasmic reticulum of neurons and selectively stains cells of several rat tissues." Journal of Histochemistry & Cytochemistry 39, no. 5 (1991): 635–43. http://dx.doi.org/10.1177/39.5.2016513.

Full text
Abstract:
Monoclonal antibody (MAb) 2H1, raised in mice immunized with membrane fractions from cultured rat pheochromocytoma cells (clonal line PC-12), detects a polypeptide from rat brain and PC-12 cell membranes of 60-65 KD apparent molecular mass. The polypeptide has been localized by immunoelectron microscopy in the rough endoplasmic reticulum (RER) of neurons. By light microscopic immunocytochemistry, several rat tissues and two rat-derived cultured cell types show selective patterns of staining with 2H1. In the central nervous system, the antibody stains neuronal cytoplasm; in the spleen, staining is seen only in certain cells of the marginal zone of the white pulp, and in lymph nodes, in plasma cells, and in areas populated by monocytes and macrophages. Whereas astrocytes and adrenal medullary cells in situ are virtually unstained with 2H1, primary cultures of astrocytes and PC-12 cells, which are derived from adrenal medullary cells, stain intensely with 2H1. The strong staining of cultured astrocytes and PC-12 cells with 2H1 suggests that the levels of the 60-65 KD polypeptide are up-regulated during cell proliferation and growth. Only a few hepatocytes stain with 2H1; intestinal epithelial and pancreatic cells are not stained with 2H1. The organelle-specific antibody 2H1 may prove a useful probe in structural and functional studies of membranes of the rough endoplasmic reticulum in neurons, and in certain cells of the immune system.
APA, Harvard, Vancouver, ISO, and other styles
6

Yonemura, S., and T. D. Pollard. "The localization of myosin I and myosin II in Acanthamoeba by fluorescence microscopy." Journal of Cell Science 102, no. 3 (1992): 629–42. http://dx.doi.org/10.1242/jcs.102.3.629.

Full text
Abstract:
We used several fixation protocols and a panel of monoclonal antibodies to re-examine the localization of myosin I and myosin II in Acanthamoeba. Two monoclonal antibodies that bind to the head of myosin II stain a range of particles in the cytoplasm. The smallest and most numerous cytoplasmic particles are about the same size and intensity as myosin II minifilaments and are distributed throughout the endoplasm. The largest particles stain like myosin II thick filaments and are concentrated in the cleavage furrow of dividing cells and in the tail of locomoting cells. Five different monoclonal antibodies that bind to the myosin II tail also stain cytoplasmic particles but with a limited range of intensity. None of the myosin II monoclonal antibodies stains the contractile vacuole or plasma membrane. Two monoclonal antibodies to myosin I gave punctate cytoplasmic staining that did not correspond clearly to any of the phase-dense particles in the cytoplasm. In many, but not all, locomoting cells, the myosin I staining was concentrated at the leading edge. Both myosin I antibodies stained a single cytoplasmic vacuole of variable size that was presumed to be the contractile vacuole. The antibody that binds myosin IA but not myosin IB stained novel intercellular contacts and the antibody that binds both myosin IA and myosin IB stained the plasma membrane, especially the tips of filopodia.
APA, Harvard, Vancouver, ISO, and other styles
7

Poot, M., Y. Z. Zhang, J. A. Krämer, et al. "Analysis of mitochondrial morphology and function with novel fixable fluorescent stains." Journal of Histochemistry & Cytochemistry 44, no. 12 (1996): 1363–72. http://dx.doi.org/10.1177/44.12.8985128.

Full text
Abstract:
Investigation of mitochondrial morphology and function has been hampered because photostable, mitochondrion-specific stains that are retained in fixed, permeabilized cells have not been available. We found that in live cell preparations, the CMXRos and H2-CMXRos dyes were more photostable than rhodamine 123. In addition, fluorescence and morphology of mitochondria stained with the CMXRos and CMXRos-H2 dyes were preserved even after formaldehyde fixation and acetone permeabilization. Using epifluorescence microscopy, we showed that CMXRos and H2-CMXRos dye fluorescence fully co-localized with antibodies to subunit I of cytochrome c oxidase, indicating that the dyes specifically stain mitochondria. Confocal microscopy of these mitochondria yielded colored banding patterns, suggesting that these dyes and the mitochondrial enzyme localize to different suborganellar regions. Therefore, these stains provide powerful tools for detailed analysis of mitochondrial fine structure. We also used poisons that decrease mitochondrial membrane potential and an inhibitor of respiration complex II to show by flow cytometry that the fluorescence intensity of CMXRos and H2-CMXRos dye staining responds to changes in mitochondrial membrane potential and function. Hence, CMXRos has the potential to monitor changes in mitochondrial function. In addition, CMXRos staining was used in conjunction with spectrally distinct fluorescent probes for the cell nucleus and the microtubule network to concomitantly evaluate multiple features of cell morphology.
APA, Harvard, Vancouver, ISO, and other styles
8

Nadin, Silvina B., Laura M. Vargas–Roig, and Daniel R. Ciocca. "A Silver Staining Method for Single-cell Gel Assay." Journal of Histochemistry & Cytochemistry 49, no. 9 (2001): 1183–86. http://dx.doi.org/10.1177/002215540104900912.

Full text
Abstract:
The single-cell gel assay (comet assay) is a very useful microelectrophoretic technique for evaluation of DNA damage and repair in individual cells. Usually, the comets are visualized and evaluated with fluorescent DNA stains. This staining requires specific equipment (e.g., a high-quality fluorescence microscope), the slides must be analyzed immediately, and they cannot be stored for long periods of time. Here we describe, using human lymphocytes, some modifications of the silver staining for comets that significantly increase the sensitivity/reproducibility of the assay. This silver staining was compared with fluorescence staining and commercial silver stains. (J Histochem Cytochem 49:1183–1186, 2001)
APA, Harvard, Vancouver, ISO, and other styles
9

Mao, Chenyi, Min Yen Lee, Jing-Ru Jhan, et al. "Feature-rich covalent stains for super-resolution and cleared tissue fluorescence microscopy." Science Advances 6, no. 22 (2020): eaba4542. http://dx.doi.org/10.1126/sciadv.aba4542.

Full text
Abstract:
Fluorescence microscopy is a workhorse tool in biomedical imaging but often poses substantial challenges to practitioners in achieving bright or uniform labeling. In addition, while antibodies are effective specific labels, their reproducibility is often inconsistent, and they are difficult to use when staining thick specimens. We report the use of conventional, commercially available fluorescent dyes for rapid and intense covalent labeling of proteins and carbohydrates in super-resolution (expansion) microscopy and cleared tissue microscopy. This approach, which we refer to as Fluorescent Labeling of Abundant Reactive Entities (FLARE), produces simple and robust stains that are modern equivalents of classic small-molecule histology stains. It efficiently reveals a wealth of key landmarks in cells and tissues under different fixation or sample processing conditions and is compatible with immunolabeling of proteins and in situ hybridization labeling of nucleic acids.
APA, Harvard, Vancouver, ISO, and other styles
10

Ward, Dennis C., and Robert W. Hall. "Contrast Enhancement of Spermatozoa in Seminal Stains." Proceedings, annual meeting, Electron Microscopy Society of America 43 (August 1985): 516–17. http://dx.doi.org/10.1017/s0424820100119405.

Full text
Abstract:
The localization and visualization of intact spermatozoa in dried stains confirms the presence of semen in cases of suspected rape. Even though the average human male ejaculate contains over 240 million spermatozoa, current searching methods are often inefficient and tedious. These methods include the light microscopic search for spermatozoa following either: extraction of the stain components in aqueous buffer, destruction of the stain substrate, or the in situ staining of the suspected area.The extraction technique is most commonly employed even though it is inefficient in cell recovery. Spermatozoa apparently bind tenaciously to the support medium during the drying of the seminal plasma. The extraction process often fails in extracting complete cells. The number of spermatozoa collected from a stain may be further reduced by dilution of the semen with other body fluids, dilution of stain components with extraction medium, limited stain size, or a low sperm count due to physiological and/or elective (vasectomy) reasons.
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "Cells. Stains and staining (Microscopy)"

1

York, Robert A. "Megagametogenesis and nuclear DNA content estimation in Halophila (Hydrocaritaceae) /." Electronic version (PDF), 2005. http://dl.uncw.edu/etd/2005/yorkr/robertyork.pdf.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Litt, Lloyd C. "An investigation of electrophoresis gel silver staining using large area sample inclusive polymerization /." Online version of thesis, 1989. http://hdl.handle.net/1850/11463.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Pinidiyaarachchi, Amalka. "Digital Image Analysis of Cells : Applications in 2D, 3D and Time." Doctoral thesis, Uppsala universitet, Centrum för bildanalys, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-9541.

Full text
Abstract:
Light microscopes are essential research tools in biology and medicine. Cell and tissue staining methods have improved immensely over the years and microscopes are now equipped with digital image acquisition capabilities. The image data produced require development of specialized analysis methods. This thesis presents digital image analysis methods for cell image data in 2D, 3D and time sequences. Stem cells have the capability to differentiate into specific cell types. The mechanism behind differentiation can be studied by tracking cells over time. This thesis presents a combined segmentation and tracking algorithm for time sequence images of neural stem cells.The method handles splitting and merging of cells and the results are similar to those achieved by manual tracking. Methods for detecting and localizing signals from fluorescence stained biomolecules are essential when studying how they function and interact. A study of Smad proteins, that serve as transcription factors by forming complexes and enter the cell nucleus, is included in the thesis. Confocal microscopy images of cell nuclei are delineated using gradient information, and Smad complexes are localized using a novel method for 3D signal detection. Thus, the localization of Smad complexes in relation to the nuclear membrane can be analyzed. A detailed comparison between the proposed and previous methods for detection of point-source signals is presented, showing that the proposed method has better resolving power and is more robust to noise. In this thesis, it is also shown how cell confluence can be measured by classification of wavelet based texture features. Monitoring cell confluence is valuable for optimization of cell culture parameters and cell harvest. The results obtained agree with visual observations and provide an efficient approach to monitor cell confluence and detect necrosis. Quantitative measurements on cells are important in both cytology and histology. The color provided by Pap (Papanicolaou) staining increases the available image information. The thesis explores different color spaces of Pap smear images from thyroid nodules, with the aim of finding the representation that maximizes detection of malignancies using color information in addition to quantitative morphological parameters. The presented methods provide useful tools for cell image analysis, but they can of course also be used for other image analysis applications.
APA, Harvard, Vancouver, ISO, and other styles
4

Neuhaus, Jochen, Birgit Schröppel, Martin Dass, et al. "3D-electron microscopic characterization of interstitial cells in the human bladder upper lamina propria." Universitätsklinikum Leipzig, 2017. https://ul.qucosa.de/id/qucosa%3A15544.

Full text
Abstract:
1) Aims To explore the ultrastructure of interstitial cells in the upper lamina propria of the human bladder, to describe the spatial relationships and to investigate cell-cell contacts. 2) Methods Focused ion beam scanning electron microscopy (FIB-SEM), 3-View SEM and confocal laser scanning microscopy were used to analyze the 3D ultrastructure of the upper lamina propria in male and female human bladders. 3) Results 3View-SEM image stacks as large as 59µm x 59µm x 17µm (xyz) at a resolution of 16nm x 16nm x 50 nm and high resolution (5nm x 5nm x 10nm) FIB-SEM stacks could be analyzed. Interstitial cells with myoid differentiation (mIC) and fibroblast like interstitial cells (fIC) were the major cell types in the upper lamina propria. The flat, sheet-like ICs were oriented strictly parallel to the urothelium sheet-like morphology. No spindle shaped cells were present. We furthermore identified one branched cell (bIC) with several processes contacting urothelial cells by penetrating the basal membrane. This cell did not make any contacts to other ICs within the upper lamina propria. We found no evidence for the occurrence of telocytes in the upper lamina propria. 4) Conclusions Comprehensive 3D-ultrastructural analysis of the human bladder confirmed distinct subtypes of interstitial cells. We provide evidence for a foremost unknown direct connection between a branched interstitial cell and urothelial cells of which the functional role has still to be elucidated. 3D-ultrastructure analyses at high resolution are needed to further define the subpopulations of lamina propria cells and cell-cell interactions.
APA, Harvard, Vancouver, ISO, and other styles
5

Wiklund, Sofia. "Effects on immune cell viability, morphology and proliferation in a sub-microliter cell sampler system." Thesis, Linköpings universitet, Institutionen för fysik, kemi och biologi, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-89982.

Full text
Abstract:
Today,   most traditional method used in the research of immune cells, such as flow   cytometry and microscopy, are based on average values of cell responses.   However, immune cells are heterogeneous and respond differently to a given   stimuli. There is also a risk that important, but rare, behaviors of   individual cells are missed when a larger population of immune cells is   analyzed. Also, flow cytometry and microscopy do not allow long-term survival   of cells; these methods lack the ability to do dynamic long-term analysis of   motile immune cells, i.e. studies of cell-cell interactions, morphology and proliferation.   In a   patient who is affected by cancer, the cell heterogeneity contributes to the   ability to battle various types of cancer or virus infections. In an   outbreak, immune cells recognize and kill tumor cells. However, the number of   specific immune cells is sometimes too few to kill all the tumor cells in a   successful way. One way to help these patients is to isolate, select out and   cultivate the active immune cells with capacity to kill tumor cells.   The   Cell Physic Laboratory (a part of the department of Applied Physics) at the   Royal Institute of Technology (KTH) has developed a method for single-cell   analysis where the immune cells are trapped in microwells in a silicon chip.   The immune cells are then studied by using fluorescence microscopy in an   inverted setup. The method enables high-throughput experiments due to the   parallelization. Furthermore, since the immune cells survive long periods in   the chip, the cells can be analyzed over several days up to weeks. The   research group has also developed a semi-automatic ‘cell-picker’. The   cell-picker will be used in combination with the developed method for   single-cell analysis, which enables picking of cells of interest. In this report, experiments for the characterization and evaluation of the biocompatibility of two generations of the cell-picker will be presented. The experiments include development of a protocol for the cell-picking process, studies of the survival time of transferred cells for both generation of the cell-picker and studies of surface coating in the chip in order to increase the biocompatibility. The preliminary results indicate that the cell-picker has potential to be used as a selection tool for immune cells of interest.
APA, Harvard, Vancouver, ISO, and other styles
6

Stanback, Cheryl E. "Immunohistochemical demonstration of epithelial differentiation antigens and HLA-DR antigens in white lesions of the oral mucous membranes a thesis submitted in partial fulfillment ... oral pathology and diagnosis ... /." 1987. http://books.google.com/books?id=7FE_AAAAMAAJ.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

ĎURINOVÁ, Eva. "Evaluation of negative stains for single particle analysis in electron microscopy." Master's thesis, 2018. http://www.nusl.cz/ntk/nusl-375359.

Full text
Abstract:
Four negative stains, hafnium chloride and europium, samarium and gadolinium nitrates, were tested in single particle electron microscopy as potential alternatives to uranyl acetate, which is recently being widely restricted for its toxicity. The new stains were applied to a structurally well-described plant photosystem I, visualized by a transmission electron microscope and classified in a single particle analysis. The quality of the stains was evaluated by the obtained resolution and ability to provide reliable structural information.
APA, Harvard, Vancouver, ISO, and other styles
8

Achilonu, Ikechukwu Anthony. "Dye-protein interactions : protein staining and dye-IgY, dye-dextran-IgY complexes for antigen detection." Thesis, 2004. http://hdl.handle.net/10413/10109.

Full text
Abstract:
In order to develop a cheaper alternative to the conventional enzyme-linked immunosorbent assay system, application of dye molecules as labels in immunoassay was investigated in this study. This chromogenic dye-antibody conjugate could be used in colourimetric immunodetection diagnostic assays that could be used in a rural African setting. The chemistry of the interaction between twenty-six dyes of anionic, cationic and ligand dye classes with IgY and other proteins were studied for protein detection and conjugation to antibodies. Out of the twenty-six dyes studied, Direct Red 81 proved to be a good protein stain on nitrocellulose and polyacrylamide gels with comparable sensitivity to Coomassie Blue R 250. Direct Red stained proteins faster (< 5 min) than Coomassie Blue R 250 in polyacrylamide gels. Aurintricarboxylic Acid, Ethyl Red and Gallocyanine with carboxylic acid and/or hydroxyl functional groups were selected, activated with carbonyldiimidazole (CDI) to form amine reactive-imidazole intermediates and conjugated to anti-rabbit albumin IgY. Gallocyanine gave the best molar coupling ratio with IgY (76:1 dye:IgY). The dye-antibody conjugates were used to detect rabbit albumin on nitrocellulose. Aurintricarboxylic Acid-IgY and Gallocyanine-IgY detected 50 ng of rabbit albumin on nitrocellulose, which was 10 fold less sensitive than HRPO-IgY conjugate. Cross-linking of the antibodies by the dyes compromised the immunoreactivity of the Aurintricarboxylic Acid-IgY and Gallocyanine-IgY conjugates. The immunoreactivity of Ethyl Red-IgY was not compromised. Anti-rabbit albumin IgY was conjugated to derivatized dextran as an alternative immunoassay reagent and used to detect rabbit albumin on nitrocellulose by staining the polysaccharide (dextran) in the immune complex with PAS reagent. IgY-dextran complex was able to detect 25 ng of rabbit albumin on nitrocellulose, but PAS staining resulted in high background staining of the nitrocellulose membrane. Dextran-antibody conjugates may have better potential as immunodetecting reagent than dye-IgY conjugates, if a more sensitive and specific method of detecting the dextran in the Ag:Ab-dextran immune complex is developed.
Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2004.
APA, Harvard, Vancouver, ISO, and other styles
9

Rainwater, John Chance 1979. "Dyes and indicators in molecular sensing ensembles : progress toward novel uses of dendrimers and reactands in optical sensing methods." 2008. http://hdl.handle.net/2152/18095.

Full text
Abstract:
Over the past two decades, the field of molecular sensing has developed into a mature offshoot of molecular recognition, and sensing protocols based on optical signal modulations have enjoyed particularly great success. Such sensing methods are the focus of this dissertation, in which efforts toward the integration of dendrimers and reactands into separate, optically-based sensing platforms are described. To this end, Chapter 1 provides a brief introduction to molecular sensing and its supramolecular underpinnings. The remainder of Chapter 1 is dedicated to dendrimers and their application to molecular recognition and sensing. A discussion of the physicochemical properties of dendrimers is also included to lend perspective on the structure, size, and shape of these macromolecules. The role of dyes and indicators in the elucidation of dendritic structure and function is given special consideration. Finally, selected reports of dendrimers in molecular recognition and optical sensing are summarized. Chapter 2 details original research directed toward the incorporation of dendrimers into molecular sensing ensembles. This use of dendrimers in molecular recognition and sensing is distinguished from those examples described in Chapter 1 by its modular nature. This modularity is achieved through the use of a non-covalent sensing motif based on indicator displacement. The identification and optimization of the appropriate components for use in such dendrimer-based sensing ensembles represents a contribution of the research described herein. An evaluation of indicator dyes for their incorporation into an enantioselective indicator displacement assay (eIDA) for common organic molecules is the subject of the research discussed in Chapter 3. The selected indicator dyes were assessed for use in a novel eIDA that relies on covalent bond formation for the enantioselective signaling of monofunctional organic analytes. A survey of colorimetric methods for the identification and discrimination of amines is included because these compounds served as an initial target in the proposed assay. Optical enantiosensing strategies are also reviewed in light of their relevance to the present work.
text
APA, Harvard, Vancouver, ISO, and other styles

Books on the topic "Cells. Stains and staining (Microscopy)"

1

Stains and cytochemical methods. Plenum Press, 1993.

APA, Harvard, Vancouver, ISO, and other styles
2

D, Bancroft John, ed. Troubleshooting histology stains. Churchill Livingstone, 1998.

APA, Harvard, Vancouver, ISO, and other styles
3

Royal Microscopical Society (Great Britain), ed. Negative staining and cryoelectron microscopy: The thin film techniques. BIOS Scientific Publishers in association with the Royal Microscopical Society, 1997.

APA, Harvard, Vancouver, ISO, and other styles
4

Tice, Raymond R. Aneuploidy test development: Kinetochore staining in mammalian systems. U.S. Environmental Protection Agency, Office of Health and Environmental Assessment, 1986.

APA, Harvard, Vancouver, ISO, and other styles
5

Understanding histochemistry: Selection, evaluation, and design of biological stains. E. Horwood, 1988.

APA, Harvard, Vancouver, ISO, and other styles
6

Boon, Mathilde E. Routine cytological staining techniques: Theoretical background and practice. Macmillan, 1985.

APA, Harvard, Vancouver, ISO, and other styles
7

P, Knight D., ed. Cytochemical staining methods for electron microscopy. Elsevier, 1992.

APA, Harvard, Vancouver, ISO, and other styles
8

S, Drijver Johanna, ed. Routine cytological staining techniques: Theoretical background and practice. Macmillan, 1986.

APA, Harvard, Vancouver, ISO, and other styles
9

Romhányi, György. Topooptikai reakciók és szerepük a biológiai ultrastruktúra-kutatásokban: Akadémiai székfoglaló, 1983. március 25. Akadémiai Kiadó, 1988.

APA, Harvard, Vancouver, ISO, and other styles
10

Luna, Lee G. Histopathologic methods and color atlas of special stains and tissue artifacts. American Histolabs, 1992.

APA, Harvard, Vancouver, ISO, and other styles
More sources

Book chapters on the topic "Cells. Stains and staining (Microscopy)"

1

Harrison, Michael. "Microscopy Skills: Cell Counts, Gram Stains, Ziehl-Neelsen Staining (ZN) and Blood Films." In Revolutionizing Tropical Medicine. John Wiley & Sons, Inc., 2019. http://dx.doi.org/10.1002/9781119282686.ch17.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Harris, James. "Staining MIF in Cells for Confocal Microscopy." In Macrophage Migration Inhibitory Factor. Springer US, 2019. http://dx.doi.org/10.1007/978-1-4939-9936-1_8.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Ellis, E. Ann. "Staining Sectioned Biological Specimens for Transmission Electron Microscopy: Conventional and En Bloc Stains." In Methods in Molecular Biology. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-776-1_4.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

ROLAND, J. C., and B. VIAN. "General Preparation and Staining of Thin Sections." In Electron Microscopy of Plant Cells. Elsevier, 1991. http://dx.doi.org/10.1016/b978-0-12-318880-9.50006-5.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

WILANDER, ERIK, and MONALILL LUNDQVIST. "Sequential Immunocytochemical and Silver Staining of Neuroendocrine Cells in the Same Section." In Correlative Microscopy in Biology. Elsevier, 1987. http://dx.doi.org/10.1016/b978-0-12-333922-5.50056-9.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

"Techniques of medical sciences." In Oxford Handbook of Medical Sciences, edited by Robert Wilkins, Ian Megson, and David Meredith. Oxford University Press, 2021. http://dx.doi.org/10.1093/med/9780198789895.003.0015.

Full text
Abstract:
‘Techniques of medical sciences’ brings together a summary of the major techniques used experimentally in biomedical science research. These include those for molecular genetics (electrophoresis, DNA cloning and sequencing, and techniques for DNA and RNA research), proteomics (protein extraction, mass spectrometry, structural proteomics, and microscopy), cytology and histology including immunohistochemistry, microbiology (stains, cultures, serology, molecular techniques of microbiology, and testing for antibiotic resistance), biochemical assays (photometry and spectrophotometry, radioimmunoassay, ELISA, chromatography, flow cytometry, and fluorescence-activated cell sorting), and functional studies (in vitro, ex vivo, in vivo, stem cells, and clinical studies).
APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "Cells. Stains and staining (Microscopy)"

1

Schaub, R. G., and F. P. Bell. "LIPID ACCUMULATION AND METABOLISM IN CARRAGEENAN-INDUCED GRANULOMAS COMPARED TO BLOOD MONOCYTES AND THE AORTA." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643410.

Full text
Abstract:
Arteries undergoing atherogenic change show an increase in cholesteryl esterifying activity by acylCoA:cholesterol acetyl-transferase (ACAT) and a progressive accumulation of cholesterol esters within monocyte derived foam cells. The study of these factors, however, is limited by the necessity of obtaining artery tissues for analysis. In this study, an in vivo model (Am J Path 118:134 and 120:391, 1985) which permits the analysis of foam cell development without requiring collection of aortas was examined in more detail. New Zealand rabbits (6 each) were either maintained on a 1% cholesterol/peanut oil diet (HD) or a regular chow diet (RD) for 2 weeks after which each had 15 ml of a 1% carra-geenan gel (Marine Colloids) injected subcutaneously into the mid-abdominal area. The rabbits were maintained on their respective diets for an additional 4 weeks. At sacrifice, blood was collected for both serum and monocyte isolation. Granulomas and aortic arches were also excised. Tissues were assayed for lipid accumulation and metabolism. Electron and light microscopy was also performed on immersion fixed (1% glutaraldehyde) granuloma tissue. Granulomas of HD rabbits were pale yellow and averaged 36 grams, while RD granulomas were a pale red and averaged 11 grams (p less than 0.05). RD granulomas did not stain with oil red 0. HD granulomas had homogenous oil red 0 staining which indicated lipid accumulation. Both RD and HD granulomas had large numbers of macrophages. RD macrophages accumulated follicular carrageenan, but not lipid. In HD granulomas, foam cell development was observed. Granuloma lipid content and metabolism paralleled the aorta and blood monocytes. The HD tissue had increased ACAT activity and lipid composition changes indicative of atherosclerosis. RD granulomas had no elevation of lipid content or ACAT activity. The results suggest that the carrageenan-induced granulomas provides a useful model for studying the biochemical and morphologic changes characteristic of aortic monocyte-derived foam cells and the early arterial atherosclerotic process.
APA, Harvard, Vancouver, ISO, and other styles
2

Fan, Xin, Zhengyuan Tang, John Healy, Kevin O'Dwyer, and Bryan Hennelly. "Label-free Rheinberg staining of cells using digital holographic microscopy and spatial light interference microscopy." In Advanced Optical Imaging Technologies II, edited by P. Scott Carney, Xiao-Cong Yuan, Kebin Shi, and Michael G. Somekh. SPIE, 2019. http://dx.doi.org/10.1117/12.2538670.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Shikata, Tetsuo, Toshihiko Shiraishi, Kumiko Tanaka, Shin Morishita, and Ryohei Takeuchi. "Effects of Acceleration Amplitude and Frequency of Mechanical Vibration on Osteoblast-Like Cells." In ASME 2007 International Mechanical Engineering Congress and Exposition. ASMEDC, 2007. http://dx.doi.org/10.1115/imece2007-41797.

Full text
Abstract:
Bone formation is subject in vivo to mechanical stimulation. Although many researches for bone cells of osteoblastic lineage sensing and responding to mechanical stimulation have been reported mainly in the biochemical field, effects of mechanical stimulation on bone cells are not well understood. In this study, in order to clarify effects of acceleration amplitude and frequency of mechanical stimulation on MC3T3-E1, which is an osteoblast-like cell line derived from mouse calvaria, in the sense of mechanical vibrations, their cell proliferation, cell morphology, bone matrix generation and gene expression of alkaline phosphatase (ALP) were investigated when sinusoidal inertia force was applied to the cells. After the cells were cultured in culture plates in a CO2 incubator for one day and adhered on the cultured plane, vibrating groups of the culture plates were set on an aluminum plate attached to a exciter and cultured under sinusoidal excitation in another incubator separated from non-vibrating groups of the culture plates. Acceleration amplitude and frequency were set to several kinds of conditions. The time evolution of cell density was obtained by counting the number of cells with a hemocytometer. The cell morphology was observed with a phase contrast microscope. Calcium salts generated by the cells were observed by being stained with alizarin red S solution and their images were captured with a CCD camera. The vibrating groups for the cell proliferation and the calcium salts staining were sinusoidally excited for 24 hours a day during 28-day cultivation. Gene expression of ALP was measured by a real-time reverse transcription polymerase chain reaction (real-time RT-PCR) method. After the vibrating groups for the PCR were excited for 7 days, the total RNAs were extracted. After reverse transcription, real-time RT-PCR was performed. Gene expression for ALP and a housekeeping gene were determined simultaneously for each sample. ALP gene level in each sample was normalized to the measured housekeeping gene level. The results to be obtained are as follows. In the range from 12.5 to 200 Hz, saturation cell density for the cell proliferation shows tendency of increase as frequency decreases and ALP gene expression shows a peak to frequency at 50 Hz. Among 0, 0.25 and 0.5 G, saturation cell density and ALP gene expression show tendency of increase as acceleration amplitude increases.
APA, Harvard, Vancouver, ISO, and other styles
4

Shikata, Tetsuo, Toshihiko Shiraishi, Kumiko Tanaka, Shin Morishita, and Ryohei Takeuchi. "Effects of Amplitude and Frequency of Vibration Stimulation on Cultured Osteoblasts." In ASME 2007 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. ASMEDC, 2007. http://dx.doi.org/10.1115/detc2007-34949.

Full text
Abstract:
Mechanical stimulation to bones affects osteogenesis such as decrease of bone mass of astronauts under zero gravity, walking rehabilitation to bone fracture and fracture repair with ultrasound devices. Bone cells have been reported to sense and response to mechanical stimulation at cellular level morphologically and metabolically. In the view of mechanical vibrations, bone cells are deformed according to mechanical stimulation and their mechanical characteristics. Recently, it was reported that viscoelasticity of cells was measured using tensile and creep tests and that there was likely natural frequency and nonlinearity of cells in the sense of structural dynamics. It suggests that there is effective frequency and amplitude of mechanical stimulation on osteogenesis by bone cells. In this study, sinusoidal inertia force was applied to cultured osteoblasts, MC3T3-E1, and effects of frequency and acceleration amplitude of mechanical vibration on the cells were investigated in respect of cell proliferation, cell morphology, bone matrix generation and alkaline phosphatase (ALP) gene expression. After the cells were cultured in culture plates in a CO2 incubator for one day and adhered on the cultured plane, vibrating groups of the culture plates were set on an aluminum plate attached to a exciter and cultured under sinusoidal excitation in another incubator separated from non-vibrating groups of the culture plates. Acceleration amplitude and frequency were set to several kinds of conditions. The time evolution of cell density was obtained by counting the number of cells with a hemocytometer. The cell morphology was observed with a phase contrast microscope. Calcium salts generated by the cells were observed by being stained with alizarin red S solution and their images were captured with a CCD camera. The vibrating groups for the cell proliferation and the calcium salts staining were sinusoidally excited for 24 hours a day during 28-day cultivation. Gene expression of ALP was measured by a real-time RT-PCR method. After the vibrating groups for the PCR were excited for 6 hours, the total RNAs were extracted. After reverse transcription, real-time RT-PCR was performed. Gene expression for ALP and a housekeeping gene were determined simultaneously for each sample. Gene levels in each sample were normalized to the measured housekeeping gene levels. As a result, it is shown that saturate cell density becomes high and bone matrix generation is promoted by applying mechanical vibration and that there may be some peaks to frequency and a certain threshold value to acceleration amplitude of mechanical vibration for saturation cell density and bone matrix generation.
APA, Harvard, Vancouver, ISO, and other styles
5

Bini, A., R. Mesa-Tejada, J. Fenoglio, B. Kudryk, and K. L. Kaplan. "SPECIFIC DETECTION OF FIBRIN IN HUMAN TISSUES BY A NEW IMMUNOHISTOCHEMICAL TECHNIQUE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643316.

Full text
Abstract:
Human biopsy (30), surgical (50) and autopsy (14) specimens of different embryonic origin (skin, blood vessel, kidney, lymph nodes, prostate, lung, liver, and intestine) were stained by the avidin-biotin complex immunoperoxidase technique (ABC-IP) with monoclonal antibodies (MAbs). MAb T2G1 (recognizes 315-42 and detects fibrin II in tissues), MAb I8C6 (recognizes BS1-42 and indicates fibrinogen and fibrin I), MAb GC4 (specific for fragments D and D-D), and a polyclonal antiserum for fibrinogen. The method can be applied to frozen or Boilin’s fixed paraffin embedded tissues with good preservation of morphology and high sensitivity at the light microscopy level. The results were compared with conventional histochemical stains currently used in surgical pathology to demonstrate fibrin deposits in tissues. These stains are based on the acidophilic properties of fibrin (Fraser-Lendrum for “more recent” and Mallory’s PTAH for “older” fibrin). ABC-IP staining with MAb T2G1 clearly detected fibrin in areas where Lendrum and PTAH failed to reveal fibrin deposits, e.g., in the intercellular and pericellular matrix, as well as in areas where staining occurred with conventional techniques, indicating greater sensitivity of the ABC-IP method. Fibrin was specifically detected in strands or clumps in some areas of inflammation and granulation tissue and seemed to be associated with platelets and macrophages. Moreover, ABC-IP with MAb I8C6 and MAb GC4 permits the distinction between fibrinogen or fibrin I, and D and D dimer which are poorly reactive with the Lendrum and PTAH methods. The polyclonal antiserum for fibrinogen showed reactivity with all the material stained with the MAbs and with some additional areas due to the epitopes of fibrinogen and fibrin not detected by the monoclonals. The ABC-IP technique with MAbs allows specific demonstration of fibrin deposits in tissues. Moreover, these results indicate that this method facilitates the correlation of the morphologic appearance of fibrinogen) -related deposits in tissues with their molecular form and will be useful to elucidate the role of fibrin in diseases such as atherosclerosis, kidney disease, and tumors.
APA, Harvard, Vancouver, ISO, and other styles
6

Zhang, Di, Lusik Cherkezyan, Yue Li, et al. "Histological staining can enhance the performance of spectroscopic microscopy on sensing nanoarchitectural alterations of biological cells (Conference Presentation)." In Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIV, edited by Alexander N. Cartwright, Dan V. Nicolau, and Dror Fixler. SPIE, 2017. http://dx.doi.org/10.1117/12.2252696.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Whited, Bryce M., Matthias C. Hofmann, Chris G. Rylander, et al. "Non-Destructive Real-Time Imaging of Cell Seeded Tissue Engineered Scaffolds." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53721.

Full text
Abstract:
The use of tissue engineered scaffolds in combination with progenitor cells has emerged as a promising strategy to restore or replace tissues damaged by disease or trauma. In addition to being biocompatible and exhibiting appropriate mechanical properties, scaffolds must be designed to sustain cell attachment, proliferation, and differentiation to ultimately produce the desired tissue once implanted in the patient [1]. Conventional techniques used to assess successful scaffold design include cell viability stains, DNA assays, and histological sectioning/staining. While significant information can be gained from using these methodologies, they are destructive to the sample and only provide snapshots of scaffold and cell development at a limited number of time points. Consequently, key temporal and spatial information relating to tissue regeneration in the scaffold is lost utilizing these techniques. Thus, the ability to non-destructively monitor cell viability, proliferation, and differentiation in real-time is of great importance for scaffold design and tissue engineering [2].
APA, Harvard, Vancouver, ISO, and other styles
8

Campton, Daniel, Arturo Ramirez, Joshua Nordberg, Anthony Blau, Jackie Stilwell, and Eric Kaldjian. "Abstract 3072: High-recovery multiplex analysis of circulating tumor cells by density-based enrichment, automated platform immunofluorescence staining, and digital microscopy." In Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-3072.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Bini, A., V. D"Agati, C. Pirani, B. Kudryk, and K. L. Kaplan. "MONOCLONAL ANTIBODY IDENTIFICATION OF FIBRIN DEPOSITS IN RENAL DISEASE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643319.

Full text
Abstract:
Glomerular and vascular "fibrin" deposition has frequently been reported in human and experimental renal diseases. The biochemical form of this "fibrin" has not been well defined. We studied 16 renal biopsies (Bouin's fixed paraffin embedded) with the ABC-immunoperoxidase technique using monoclonal antibodies (MAbs); MAb I8C6 (Bβ1-42) to fibrinogen and fibrin I; MAb T2G1 (β15-42) to fibrin II; and MAb GC4 to fragment D or D-D. Polyclonal antisera to fibrinogen, albumin and IgG were used as controls. Renal biopsy specimens included 9 cases of microangiopathy (Group I: 6 hemolytic uremic syndrome (HUS), 1 sclerodero-ma, 2 acute humoral rejection) and 7 miscellaneous cases of other renal disease (Group II: 2 IgA nephropathy, 2 minimal change disease, 2 membranous GN, 1 acute interstitial nephritis). Fibrinogen and fibrin I were present on the glomerular (glom) endothelial cells in 8/9 Group I and 7/7 Group II cases, but was present in glom capillary lumens in Group I only. Staining of the endothelial aspect of interstitial capillaries, arterioles and arteries was also observed in both groups. Fibrin II was present in most glom and interstitial capillaries in both groups. However, intense staining for fibrin II was observed in arterioles and arteries in Group I only. Staining for fragments D and D-D was observed in glom capillaries in 6 Group I cases (6 HUS) and 3 Group II cases (2 IgA nephropathy, 1 membranous GN) but was most diffuse and intense in Group I. Traditional histochemical stains for fibrin (Lendrum and PTAH) were positive in 4 Group I cases only, indicating that the ABC technique is far more sensitive. Controls (14 needle biopsies of non-renal tissue) showed no vascular ractivity for fibrinogen, fibrin I, II and fragments D and D-D, suggesting that the vascular staining observed in renal biopsy tissue is not caused by the biopsy procedure itself. These findings indicate that 1) Fibrin formation and lysis occur in many renal diseases of both vascular and non—vascular origin. 2) Fibrinolytic activity is higher in the glom capillaries than in the larger vessels. 3) Damaged renal endothelium may be an active participant in these processes.
APA, Harvard, Vancouver, ISO, and other styles
10

Tang, Xin, Tony Cappa, Theresa B. Kuhlenschmidt, Mark S. Kuhlenschmidt, and Taher A. Saif. "Studying the Mechanical Sensitivity of Human Colon Cancer Cells Through a Novel Bio-MEMS Force Sensor." In ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology. ASMEDC, 2010. http://dx.doi.org/10.1115/nemb2010-13237.

Full text
Abstract:
Cancer deaths are primarily caused by metastases, not by the parent tumor. During the metastasis, malignant cancer cells detach from the parent tumor, and spread through the patient’s circulatory system to invade new tissues and organs [1]. To study the role played by the mechanical microenvironment on the cancer cell growth and malignancy promotion, we cultured human colon carcinoma (HCT-8) cells in vitro on substrates with varied mechanical stiffness, from the physiologically relevant 1 kPa, 20 kPa to very stiff 3.5 GPa. A novel and versatile micro-electromechanical systems (Bio-MEMS) force sensor [2] is developed to quantify the strength of non-specific adhesion between living cancer cells membrane and probe, an important hallmark of cancer cell malignancy level. Immunoflurescent staining and Confocal microscopy imaging are used to visualize the cellular organelle organization and cooperate to explore the underlying mechanism.
APA, Harvard, Vancouver, ISO, and other styles
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography