Relevant bibliographies by topics / Celluclast

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Academic literature on the topic 'Celluclast'

Author: Grafiati
Published: 4 June 2025
Last updated: 23 June 2025

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Journal articles on the topic "Celluclast"

1

Yeh, Yu-Cheng, and Lih-Shiuh Lai. "Effect of Extraction Procedures with Ultrasound and Cellulolytic Enzymes on the Structural and Functional Properties of Citrus grandis Osbeck Seed Mucilage." Molecules 27, no. 3 (2022): 612. http://dx.doi.org/10.3390/molecules27030612.

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The structural and functional properties of Citrus grandis Osbeck (CGO) seed mucilage by different extraction practices, including conventional citrate buffer, ultrasonic-assisted (UAE), enzymatic-assisted extraction (EAE) with cellulase or Celluclast® 1.5 L and various ultrasonic-assisted enzymatic extraction (UAEE) procedures were investigated. It was found that CGO seed from agricultural and processing byproducts is an excellent new source of high methoxyl pectin with quite high intrinsic viscosity (about 108.64 dL/g) and molecular weight (about 1.9 × 106) as compared with other pectin sources. UAEE with Celluclast® 1.5 L enhanced the extraction yield most pronouncedly (about 2.3 times). Moreover, the monosaccharide composition of CGO seed mucilage is least affected by EAE with Celluclast® 1.5 L. In contrast, EAE with cellulase dramatically reduces the galacturonic acid (GalA) content to less than 60 molar%, and increases the glucose (Glc) content pronouncedly (to about 40 molar%), which may be considered as an adverse effect in terms of pectin purity. Though extraction procedures involved with ultrasound and cellulolytic enzymes generally show a decrease in GalA contents, weight average molar mass and intrinsic viscosity, EAE with Celluclast® 1.5 L is least affected, followed by UAE and UAEE with Celluclast® 1.5 L. These features can be leveraged in favor of diversified applications.
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2

Yang, Fengqi, Jimin Hyun, D. P. Nagahawatta, Young Min Kim, Moon-Soo Heo, and You-Jin Jeon. "Cosmeceutical Effects of Ishige okamurae Celluclast Extract." Antioxidants 11, no. 12 (2022): 2442. http://dx.doi.org/10.3390/antiox11122442.

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Sulfated polysaccharides extracted from brown algae are unique algal polysaccharides and potential ingredients in the cosmeceutical, functional food, and pharmaceutical industries. Therefore, the present study evaluated the cosmeceutical effects, including antioxidant, anti-wrinkle, anti-inflammation, and photoprotective activities, of Ishige okamurae Celluclast extract (IOC). The IOC was abundant in sulfated polysaccharides (48.47%), polysaccharides (44.33%), and fucose (43.50%). Moreover, the IOC effectively scavenged free radicals, and its anti-inflammatory properties were confirmed in lipopolysaccharide-induced RAW 264.7 macrophages; therefore, the IOC may produce auxiliary effects by inhibiting reactive oxygen species (ROS). In vitro (Vero cells) and in vivo (zebrafish) studies further confirmed that the IOC produced a protective effect against hydrogen-peroxide-induced oxidative stress in a dose-dependent manner. In addition, the IOC suppressed intracellular ROS and apoptosis and enhanced HO-1 and SOD-1 expression through transcriptional activation of Nrf2 and downregulation of Keap1 in HaCaT cells. Furthermore, the IOC exhibited a potent protective effect against ultraviolet-B-induced skin damage and photoaging. In conclusion, the IOC possesses antioxidant, anti-inflammatory, and photoprotective activities, and can, therefore, be utilized in the cosmeceutical and functional food industries.
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Zeghlouli, Jihane, Gwendoline Christophe, Amine Guendouz, et al. "Optimization of Bioethanol Production from Enzymatic Treatment of Argan Pulp Feedstock." Molecules 26, no. 9 (2021): 2516. http://dx.doi.org/10.3390/molecules26092516.

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Argan pulp is an abundant byproduct from the argan oil process. It was investigated to study the feasibility of second-generation bioethanol production using, for the first time, enzymatic hydrolysis pretreatment. Argan pulp was subjected to an industrial grinding process before enzymatic hydrolysis using Viscozyme L and Celluclast 1.5 L, followed by fermentation of the resulting sugar solution by Saccharomyces cerevisiae. The argan pulp, as a biomass rich on carbohydrates, presented high saccharification yields (up to 91% and 88%) and an optimal ethanol bioconversion of 44.82% and 47.16% using 30 FBGU/g and 30 U/g of Viscozyme L and Celluclast 1.5 L, respectively, at 10%w/v of argan biomass.
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Wikiera, Agnieszka, Magdalena Mika, Anna Starzyńska-Janiszewska, and Bożena Stodolak. "Application of Celluclast 1.5L in apple pectin extraction." Carbohydrate Polymers 134 (December 2015): 251–57. http://dx.doi.org/10.1016/j.carbpol.2015.07.051.

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Morais, Jéssica R. F., Isabela O. Costa, Carlos E. A. Padilha, Nathália S. Rios, and Everaldo S. dos Santos. "Improving Reusability of Biocatalysts by Exploiting Cross-Linked Enzyme Aggregates (CLEAs) with Commercial Cellulolytic Cocktails for Hydrolysis of Green Coconut Waste." Sustainability 17, no. 9 (2025): 4221. https://doi.org/10.3390/su17094221.

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Efficient hydrolysis of cellulose in agricultural waste (e.g., coconut fiber) is critical for biorefining processes such as second-generation bioethanol (2G ethanol) production. However, free cellulases suffer from low thermal stability and challenges in recovery. To address this, we developed cross-linked enzyme aggregates (CLEAs) combined with magnetic nanoparticles (magnetic CLEAs, m-CLEAs) to enhance enzyme stability and reusability. In this context, solutions of ethanol, acetone, and ammonium sulfate were used to prepare enzymatic aggregates, with subsequent use of glutaraldehyde and magnetic nanoparticles to obtain the biocatalysts. The addition of bovine serum albumin (BSA) protein was also tested to improve immobilization. Biocatalysts with ethanol and acetone performed better. Acetone (AC) and BSA yielded the highest enzymatic activities (287.27 ± 42.59 U/g for carboxymethyl cellulase (CMCase) with Celluclast; 425.37 ± 48.11 U/g for CMCase with Cellic CTec2). Magnetic nanoparticles were incorporated to expand the industrial applicability, producing m-CLEAs with excellent thermal stability and high catalytic activities. The m-CLEA–Celluclast–AC–BSA–GA 5% maintained 58% of its activity after 72 h at 70 °C. The m-CLEA–Celluclast-AC–BSA–GA 2.5% proved effective in hydrolyzing coconut fiber and isolated cellulose, producing up to 0.91 ± 0.01 g/L of glucose and 2.7 ± 0.15 g/L of glucose, respectively, after 72 h. Therefore, this approach supports sustainability by using coconut fiber, which is often discarded into the environment.
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Jagelavičiutė, Jolita, Dalia Čižeikienė, and Loreta Bašinskienė. "Enzymatic Modification of Apple Pomace and Its Application in Conjunction with Probiotics for Jelly Candy Production." Applied Sciences 15, no. 2 (2025): 599. https://doi.org/10.3390/app15020599.

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This study aimed to evaluate the applicational possibilities of enzymatically modified apple pomace (AP) in conjunction with probiotics as value-added ingredients for the production of jelly candies. AP was enzymatically modified with Pectinex® Ultra Tropical, Viscozyme® L, and Celluclast® 1.5 L (Novozyme A/S, Bagsværd, Denmark), and the soluble and insoluble dietary fibre content was determined using the Megazyme kit (Megazyme International Ireland Ltd., Wicklow, Ireland), reducing sugar content using the 3,5-dinitrosalicylic acid assay. The technological properties of the modified AP, such as its swelling capacity, water-retention capacity, oil-retention capacity, bulk density, and static and thermal emulsion stability, were evaluated. Enzymatically modified AP hydrolysed with Celluclast ® 1.5 L was used for the production of jelly candies supplemented with Bifidobacterium animalis DSM 20105. The survival of probiotics in the jelly candies during in vitro digestion, the viability of probiotics during candy storage, and candy quality characteristics were analysed. Enzymatically modified AP had different carbohydrate compositions and technological properties, depending on the enzyme preparation used. Although the viability of probiotics in the jelly candies decreased during storage, a significantly higher viability of B. animalis was determined in jelly candies supplemented with hydrolysed AP compared with control candies made without AP after digestion in the saline, gastric, and intestine phases. This study shows that Celluclast® 1.5 L can be used for increasing the soluble dietary fibre in AP (18.4%), which can be further applied, in conjunction with B. animalis, for added-value jelly candy production.
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7

Baruque, Julia R. S., Adriano Carniel, Júlio C. S. Sales, Bernardo D. Ribeiro, Rodrigo P. do Nascimento, and Ivaldo Itabaiana. "Immobilization of Cellulolytic Enzymes in Accurel® MP1000." Reactions 4, no. 2 (2023): 311–28. http://dx.doi.org/10.3390/reactions4020019.

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Cellulases are a class of enzymes of great industrial interest that present several strategic applications. However, the high cost of enzyme production, coupled with the instabilities and complexities of proteins required for hydrolytic processes, still limits their use in several protocols. Therefore, enzyme immobilization may be an essential tool to overcome these issues. The present work aimed to evaluate the immobilization of cellulolytic enzymes of the commercial enzyme cocktail Celluclast® 1.5 L in comparison to the cellulolytic enzyme cocktail produced from the wild strain Trichoderma harzianum I14-12 in Accurel® MP1000. Among the variables studied were temperature at 40 °C, ionic strength of 50 mM, and 72 h of immobilization, with 15 m·L −1 of proteins generated biocatalysts with high immobilization efficiencies (87% for ACC-Celluclast biocatalyst and 95% for ACC-ThI1412 biocatalyst), high retention of activity, and specific activities in the support for CMCase (DNS method), FPase (filter paper method) and β-glucosidase (p-nitrophenyl-β-D-glucopyranoside method). Presenting a lower protein concentration (0.32 m·L−1) than the commercial Celluclast® 1.5 L preparation (45 m·L−1), the ACC-ThI1412-derived immobilized biocatalyst showed thermal stability at temperatures higher than 60 °C, maintaining more than 90% of the residual activities of FPase, CMCase, and β-glucosidase. In contrast, the commercial-free enzyme presented a maximum catalytic activity at only 40 °C. Moreover, the difference in molecular weight between the component enzymes of the extract was responsible for different hydrophobic and lodging interactions of proteins on the support, generating a robust and competitive biocatalyst.
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Jagelaviciute, Jolita, Loreta Basinskiene, Dalia Cizeikiene, and Michail Syrpas. "Technological Properties and Composition of Enzymatically Modified Cranberry Pomace." Foods 11, no. 15 (2022): 2321. http://dx.doi.org/10.3390/foods11152321.

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Cranberry pomace obtained after juice production is a good source of dietary fiber and other bioactive compounds. In this study, cranberry pomace was hydrolyzed with Viscozyme® L, Pectinex® Ultra Tropical, Pectinex® Yieldmash Plus, and Celluclast® 1.5L (Novozyme A/S, Denmark). The soluble and insoluble dietary fiber was determined using the Megazyme kit, while the changes in mono-, disaccharide and oligosaccharides’ contents were determined using HPLC-RI; the total phenolic contents were determined by Folin−Ciocalteu’s Assay. Prebiotic activity, using two probiotic strains Lactobacillus acidophilus DSM 20079 and Bifidobacterium animalis DSM 20105, was investigated. The technological properties, such as hydration and oil retention capacity, were evaluated. The enzymatic treatment increased the yield of short-chain soluble saccharides. The highest oligosaccharide content was obtained using Viscozyme® L and Pectinex® Ultra Tropical. All of the tested extracts of cranberry pomace showed the ability to promote growth of selected probiotic bacteria. The insoluble dietary fiber content decreased in all of the samples, while the soluble dietary fiber increased just in samples hydrolyzed with Celluclast® 1.5L. The highest content of total phenolic compounds was obtained using Viscozyme® L and Pectinex® Ultra Tropical (10.9% and 13.1% higher than control, respectively). The enzymatically treated cranberry pomace exhibited lower oil and water retention capacities in most cases. In contrast, water swelling capacity increased by 23% and 70% in samples treated with Viscozyme® L and Celluclast® 1.5L, respectively. Enzymatically treated cranberry pomace has a different composition and technological properties depending on the enzyme used for hydrolysis and can be used in various novel food products.
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9

Figueiredo, M. De, and J. P. Marais. "The effect of bacterial inoculants on kikuyu silage quality." Journal of Agricultural Science 122, no. 1 (1994): 53–60. http://dx.doi.org/10.1017/s0021859600065795.

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SUMMARYTwo commercial bacterial inoculants (Lacto-flora and Ecosyl) were added to kikuyu grass (Pennisetum clandestinum) at ensiling, in 1985 and 1988 respectively, at Cedara, South Africa, using laboratory silos. In 1985 (Expt 1), Lacto-flora was added on its own and in combination with the enzymes Celluclast or SP249. In 1988 (Expt 2), Ecosyl was added on its own and in combination with molasses, at two levels of addition.The addition of Lacto-flora alone did not significantly increase the lactic acid bacteria or the lactic acid content of the treated silage. However, treated silage contained 53·6% less iso-butyric acid and 53·7% less ammonia than the control silage. Kikuyu silage supplemented with a combination of Lacto-flora and Celluclast or SP249 had higher numbers of lactic acid bacteria at ensiling (0·231 × 108/ml) than the control silage or silage receiving Lacto-flora alone. However, only silage supplemented with the combination of Lacto-flora and Celluclast had a significantly higher lactic acid content (2·23 compared with 0·04, 0·18 and 0·13% DM for the control silage, silages with Lactoflora and with a combination of Lacto-flora +SP249, respectively). Nevertheless, this silage contained 19·78% more acetic than lactic acid.Silage supplemented with Ecosyl on its own did not differ significantly in digestibility, loss of dry matter, ammonia, total non-structural carbohydrates, lactic acid and crude protein, from the untreated silage. A significant decrease in silage pH (from 5·08 to 4·70) was observed when Ecosyl was added together with molasses at the higher level of addition only. However, no other benefit was obtained by the addition of Ecosyl in combination with molasses.
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Dourado, F., M. Bastos, M. Mota, and F. M. Gama. "Studies on the properties of Celluclast/Eudragit L-100 conjugate." Journal of Biotechnology 99, no. 2 (2002): 121–31. http://dx.doi.org/10.1016/s0168-1656(02)00178-5.

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Book chapters on the topic "Celluclast"

1

Cid-Ibarra, Gabriela P., María G. Rodríguez-Delgado, Eva L. Fernández-Rodríguez, et al. "Research Progress on Application of Celluclast® as a Processing Aid for Pectin Extraction from Kiwifruit Pomace: A Mini Review." In Natural Food Products and Waste Recovery. Apple Academic Press, 2021. http://dx.doi.org/10.1201/9781003144748-8.

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Conference papers on the topic "Celluclast"

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Salleh, Noor Shafryna, and Abdul Munir Abdul Murad. "Enzymatic hydrolysis of oil palm empty fruits bunch fiber using Celluclast® and Accellerase® BG for sugar production." In THE 2016 UKM FST POSTGRADUATE COLLOQUIUM: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2016 Postgraduate Colloquium. Author(s), 2016. http://dx.doi.org/10.1063/1.4966738.

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Castejon, Natalia. "Eco-friendly Strategies to Produce Bioactive Lipids from the omega-3 Rich Microalga Nannochloropsis Gaditana." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/rwfn7404.

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Microalgae are considered a promising alternative source of omega-3 long chain-polyunsaturated fatty acids (ω-3 LC-PUFAs) since they are the primary producers of eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids in the marine environment. Extraction methods commonly used for the isolation of these bioactives are based on conventional techniques, which imply the use of high volumes of organic solvents and high energy requirements, producing health and environmental problems. In this sense, greener alternatives need to be developed to meet the global consumer demand for natural ingredients and eco-friendly products. In this work, the use of ultrasonic-assisted enzymatic extraction (UAEE) technology in combination with environmentally friendly solvents was evaluated as a green strategy to efficiently extract the omega-3 lipids from the microalga Nannochloropsis gaditana. The microalgal biomass was pretreated with different commercial enzymes (Viscozyme® L, Celluclast® 1.5 L, and Saczyme® Yield) and the results were compared with the traditional Folch method (2:1 chloroform/methanol). A promising extraction approach was developed using Saczyme® Yield and ethanol as solvent, achieving a lipid yield of 25.7% ± 0.5, comparable to the results obtained with the traditional method (27.3% ± 0.7) (p > 0.05). Similar omega-3 content was found by GC-MS analysis for both lipid extracts (30.2% ± 2.9 and 29.2% ± 1.0 for the green and the traditional method, respectively), showing that the environmentally friendly approaches did not negatively affect the fatty acid profile. Additionally, the bioactivity of the produced lipids was investigated by a spectrum of in vitro cell-based assays measuring potential endpoints of interest like cytotoxicity, antioxidant and anti-inflammatory activities. In conclusion, this work provides relevant results for new eco-friendly extraction approaches to produce functional omega-3 lipids with potential applications in the food industry, avoiding the use of toxic solvents and reducing the environmental impact.
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Reports on the topic "Celluclast"

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Teeradakorn, Siriluk. Pretreatment and hydrolysis conditioning process of cellulosic material for bioethanol production. Chulalongkorn University, 2012. https://doi.org/10.58837/chula.res.2012.81.

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Sweet sorghum straw is lignocellulosic material that is promoted as an alternative feedstock for ethanol production because it is available and inexpensive. Due to its composition of cellulose and hemicelluloses, that could be hydrolyzed into fermentable sugars. The composition of sweet sorghum straw used in this study consists of 44.51% cellulose, 38.12% hemicelluloses and 6.18% lignin. Conversion of this potential feedstock requires a pretreatment step to alter the microscopic size and structure of the lignocelluloses. This research was studied in order to find the optimum conditions on hydrolysis of sweet sorghum straw. The biomass was mixed with dilute sulfuric acid (0-3%v/v) with solid loading of 10% w/v and then pretreatment at high temperatures (120-190°C) for 10-30 min of pretreated times. The maximum vield of glucose and xylose from sweet sorghum straw was 0.234 g glucose/g dry substrate and 0.208 g xylose/g dry substrate, respectively, at the pretreatment condition: 120°C, 3%H2So4 for 10 min. After chemical pretreatment, the pretreated sweet sorghum straw was hydrolyzed with commercial cellulose. Four variables of saccharification condition were investigated; a substrate concentration (1-7%), cellulose concentration (Celluclast 1.5, Novozyme) (15-35 FPU/g substrate), a temperature (30-70°C) and a pH (3-7). The optimum conditions were 1% of substrate concentration, 15 FPU/g-substrate of cellulose, at temperature 40°C and pH of 5. Obviously, glucose was the only monosugar detectable with the yield of 0.344 g glucose/g dry solid under this saccharification condition. Monosugars liberated from the pretreated sweet sorghum straw and the saccharified pretreated sweet sorghum straw was used as carbon source for ethanol fermentation by Saccharomyces cerevisiae. Fermentation condition was at 30°C, pH 5.5 and agitation rate of 150 rpm. The high yield of ethanol concentration, of 15.40 g-ethanol/100 g-total sugars after 12 h of cultivation was obtained when using monosugar liberated from the saccharified acid pretreated sweet sorghum straw as substrate.
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