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Journal articles on the topic 'Celluclast'

Author: Grafiati

Published: 4 June 2025

Last updated: 1 August 2025

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1

Yeh, Yu-Cheng, and Lih-Shiuh Lai. "Effect of Extraction Procedures with Ultrasound and Cellulolytic Enzymes on the Structural and Functional Properties of Citrus grandis Osbeck Seed Mucilage." Molecules 27, no. 3 (2022): 612. http://dx.doi.org/10.3390/molecules27030612.

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The structural and functional properties of Citrus grandis Osbeck (CGO) seed mucilage by different extraction practices, including conventional citrate buffer, ultrasonic-assisted (UAE), enzymatic-assisted extraction (EAE) with cellulase or Celluclast® 1.5 L and various ultrasonic-assisted enzymatic extraction (UAEE) procedures were investigated. It was found that CGO seed from agricultural and processing byproducts is an excellent new source of high methoxyl pectin with quite high intrinsic viscosity (about 108.64 dL/g) and molecular weight (about 1.9 × 106) as compared with other pectin sour

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2

Yang, Fengqi, Jimin Hyun, D. P. Nagahawatta, Young Min Kim, Moon-Soo Heo, and You-Jin Jeon. "Cosmeceutical Effects of Ishige okamurae Celluclast Extract." Antioxidants 11, no. 12 (2022): 2442. http://dx.doi.org/10.3390/antiox11122442.

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Sulfated polysaccharides extracted from brown algae are unique algal polysaccharides and potential ingredients in the cosmeceutical, functional food, and pharmaceutical industries. Therefore, the present study evaluated the cosmeceutical effects, including antioxidant, anti-wrinkle, anti-inflammation, and photoprotective activities, of Ishige okamurae Celluclast extract (IOC). The IOC was abundant in sulfated polysaccharides (48.47%), polysaccharides (44.33%), and fucose (43.50%). Moreover, the IOC effectively scavenged free radicals, and its anti-inflammatory properties were confirmed in lipo

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3

Zeghlouli, Jihane, Gwendoline Christophe, Amine Guendouz, et al. "Optimization of Bioethanol Production from Enzymatic Treatment of Argan Pulp Feedstock." Molecules 26, no. 9 (2021): 2516. http://dx.doi.org/10.3390/molecules26092516.

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Argan pulp is an abundant byproduct from the argan oil process. It was investigated to study the feasibility of second-generation bioethanol production using, for the first time, enzymatic hydrolysis pretreatment. Argan pulp was subjected to an industrial grinding process before enzymatic hydrolysis using Viscozyme L and Celluclast 1.5 L, followed by fermentation of the resulting sugar solution by Saccharomyces cerevisiae. The argan pulp, as a biomass rich on carbohydrates, presented high saccharification yields (up to 91% and 88%) and an optimal ethanol bioconversion of 44.82% and 47.16% usin

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4

Wikiera, Agnieszka, Magdalena Mika, Anna Starzyńska-Janiszewska, and Bożena Stodolak. "Application of Celluclast 1.5L in apple pectin extraction." Carbohydrate Polymers 134 (December 2015): 251–57. http://dx.doi.org/10.1016/j.carbpol.2015.07.051.

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5

Morais, Jéssica R. F., Isabela O. Costa, Carlos E. A. Padilha, Nathália S. Rios, and Everaldo S. dos Santos. "Improving Reusability of Biocatalysts by Exploiting Cross-Linked Enzyme Aggregates (CLEAs) with Commercial Cellulolytic Cocktails for Hydrolysis of Green Coconut Waste." Sustainability 17, no. 9 (2025): 4221. https://doi.org/10.3390/su17094221.

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Efficient hydrolysis of cellulose in agricultural waste (e.g., coconut fiber) is critical for biorefining processes such as second-generation bioethanol (2G ethanol) production. However, free cellulases suffer from low thermal stability and challenges in recovery. To address this, we developed cross-linked enzyme aggregates (CLEAs) combined with magnetic nanoparticles (magnetic CLEAs, m-CLEAs) to enhance enzyme stability and reusability. In this context, solutions of ethanol, acetone, and ammonium sulfate were used to prepare enzymatic aggregates, with subsequent use of glutaraldehyde and magn

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6

Jagelavičiutė, Jolita, Dalia Čižeikienė, and Loreta Bašinskienė. "Enzymatic Modification of Apple Pomace and Its Application in Conjunction with Probiotics for Jelly Candy Production." Applied Sciences 15, no. 2 (2025): 599. https://doi.org/10.3390/app15020599.

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This study aimed to evaluate the applicational possibilities of enzymatically modified apple pomace (AP) in conjunction with probiotics as value-added ingredients for the production of jelly candies. AP was enzymatically modified with Pectinex® Ultra Tropical, Viscozyme® L, and Celluclast® 1.5 L (Novozyme A/S, Bagsværd, Denmark), and the soluble and insoluble dietary fibre content was determined using the Megazyme kit (Megazyme International Ireland Ltd., Wicklow, Ireland), reducing sugar content using the 3,5-dinitrosalicylic acid assay. The technological properties of the modified AP, such a

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7

Baruque, Julia R. S., Adriano Carniel, Júlio C. S. Sales, Bernardo D. Ribeiro, Rodrigo P. do Nascimento, and Ivaldo Itabaiana. "Immobilization of Cellulolytic Enzymes in Accurel® MP1000." Reactions 4, no. 2 (2023): 311–28. http://dx.doi.org/10.3390/reactions4020019.

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Cellulases are a class of enzymes of great industrial interest that present several strategic applications. However, the high cost of enzyme production, coupled with the instabilities and complexities of proteins required for hydrolytic processes, still limits their use in several protocols. Therefore, enzyme immobilization may be an essential tool to overcome these issues. The present work aimed to evaluate the immobilization of cellulolytic enzymes of the commercial enzyme cocktail Celluclast® 1.5 L in comparison to the cellulolytic enzyme cocktail produced from the wild strain Trichoderma h

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8

Jagelaviciute, Jolita, Loreta Basinskiene, Dalia Cizeikiene, and Michail Syrpas. "Technological Properties and Composition of Enzymatically Modified Cranberry Pomace." Foods 11, no. 15 (2022): 2321. http://dx.doi.org/10.3390/foods11152321.

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Cranberry pomace obtained after juice production is a good source of dietary fiber and other bioactive compounds. In this study, cranberry pomace was hydrolyzed with Viscozyme® L, Pectinex® Ultra Tropical, Pectinex® Yieldmash Plus, and Celluclast® 1.5L (Novozyme A/S, Denmark). The soluble and insoluble dietary fiber was determined using the Megazyme kit, while the changes in mono-, disaccharide and oligosaccharides’ contents were determined using HPLC-RI; the total phenolic contents were determined by Folin−Ciocalteu’s Assay. Prebiotic activity, using two probiotic strains Lactobacillus acidop

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9

Figueiredo, M. De, and J. P. Marais. "The effect of bacterial inoculants on kikuyu silage quality." Journal of Agricultural Science 122, no. 1 (1994): 53–60. http://dx.doi.org/10.1017/s0021859600065795.

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SUMMARYTwo commercial bacterial inoculants (Lacto-flora and Ecosyl) were added to kikuyu grass (Pennisetum clandestinum) at ensiling, in 1985 and 1988 respectively, at Cedara, South Africa, using laboratory silos. In 1985 (Expt 1), Lacto-flora was added on its own and in combination with the enzymes Celluclast or SP249. In 1988 (Expt 2), Ecosyl was added on its own and in combination with molasses, at two levels of addition.The addition of Lacto-flora alone did not significantly increase the lactic acid bacteria or the lactic acid content of the treated silage. However, treated silage containe

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10

Dourado, F., M. Bastos, M. Mota, and F. M. Gama. "Studies on the properties of Celluclast/Eudragit L-100 conjugate." Journal of Biotechnology 99, no. 2 (2002): 121–31. http://dx.doi.org/10.1016/s0168-1656(02)00178-5.

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11

Pérez Lara, Abril, Enrique Flores Andrade, Marisol Castillo Morales, et al. "Efecto de las condiciones de reacción sobre la actividad enzimática exoglucanasa en dos enzimas comerciales y su potencial uso para el pretratamiento de biomasa vegetal." Tendencias en energías renovables y sustentabilidad 2, no. 1 (2023): 343–54. http://dx.doi.org/10.56845/terys.v2i1.376.

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El principal componente de la biomasa vegetal es la celulosa, el cual es un polisacárido heterogéneo y para su hidrólisis enzimática se requiere la acción sinérgica de un conjunto de enzimas como la endoglucanasa, exoglucanasa (celobiohidrolasa) y β-glucosidasa. Por ello, el presente trabajo se enfocó en estudiar a la enzima exoglucanasa, para los cual, primero se montó el ensayo enzimático en microvolúmenes que se realizó en microplacas de 96 pozos utilizando Avicel a 1.25 % P/V como sustrato en un volumen de reacción de 200 μL. Se determinó que para cuantificar la actividad enzi

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12

Pérez Aguilar, Juan Alejandro, and Ricardo Benítez Benítez. "Aprovechamiento de tallos de Cannabis sativa por pretratamiento termoquímico e hidrólisis enzimática." Ciencia en Desarrollo 15, no. 1 (2024): 221–28. http://dx.doi.org/10.19053/01217488.v15.n1.2024.15304.

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Cannabis sativa es una planta ampliamente utilizada en Colombia para propósitos medicinales, para lo que se usa su flor, generando altas cantidades de tallos residuales. En este estudio, tallos de Cannabis se pretrataron con ácido sulfúrico o hidróxido de sodio diluido y su efecto se analizó por espectroscopía de infrarrojo (FTIR-ATR). Los sólidos remanentes se llevaron a hidrólisis enzimática usando dos mezclas enzimáticas comerciales: Celluclast 1.5 L y Cellic CTec3; se hizo seguimiento a la producción de azúcares reductores. Los pretratamientos removieron hemicelulosa y lignina, evidenciado

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13

Thư, Nguyễn Anh, Huỳnh Vũ, Đỗ Thị Như Thảo, Trần Thị An та Nguyễn Phạm Trúc Phương. "XÂY DỰNG QUY TRÌNH THUỶ PHÂN NẤM HƯƠNG (Lentinus edodes) TẠO SẢN PHẨM BẢO VỆ SỨC KHOẺ". TNU Journal of Science and Technology 228, № 05 (2023): 222–29. http://dx.doi.org/10.34238/tnu-jst.7136.

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Các nghiên cứu cho thấy nấm hương được sử dụng trong y học cho các bệnh liên quan đến suy giảm chức năng miễn dịch, ung thư, dị ứng môi trường, nhiễm nấm, cúm và cảm lạnh thường xuyên, viêm phế quản, bệnh tim, tăng lipid trong máu, tăng huyết áp, bệnh truyền nhiễm, bệnh tiểu đường, viêm gan. Do đó, nghiên cứu này được thực hiện nhằm mục tiêu xây dựng quy trình công nghệ thuỷ phân nấm hương, thu nhận phân đoạn có hoạt tính sinh học. Kết quả nghiên cứu đã xây dựng thành công quy trình thuỷ phân chân nấm hương với các thông số: nguyên liệu chân nấm hương 7,27g, enzyme Celluclast 1,3 mL và Flavour

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14

คตินนท์กุล, วัชรี, та เจนจิรา ภูริรักษ์พิติกร. "การปรับสภาพเปลือกข้าวโพดและเปลือกมะพร้าวด้วยของเหลวไอออนิก เพื่อเพิ่มผลผลิตน้ำตาลกลูโคส". วารสารวิทยาศาสตร์ประยุกต์ กรมวิทยาศาสตร์บริการ 2, № 2 (2013): 26–34. http://dx.doi.org/10.60136/bas.v2.2013.267.

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การศึกษานี้แสดงการปรับสภาพโดยใช้ของเหลวไอออนิกในวัสดุประเภทลิกโนเซลลูโลส ได้แก่ เปลือกข้าวโพด และเปลือกมะพร้าว ทั้งนี้เพื่อเพิ่มประสิทธิภาพร้อยละการคืนกลับของน้ำตาลกลูโคสโดยของเหลวไอออนิกที่ใช้ศึกษา มีสองชนิดคือ 1-บิวทิล-3-เมทิลอิมิดาโซเดียมคลอไรด์ (1-butyl-3-methylimidazolium chloride, BmimCl) และ1-เอทิล-3-เมทิลอิมิดาโซเลียมอะซิเทต (1-ethyl-3-methylimidazolium acetate, EmimOAC) ภายหลังจากที่ วัสดุผ่านการปรับสภาพแล้วจะถูกนําไปย่อยด้วยเอนไซม์โดยเอนไซม์ที่ใช้คือ Celluclast 1.5L และ Novozymes 188 ซึ่งพบว่าสภาวะการปรับสภาพที่มีประสิทธิภาพสูงสุดสําหรับเปลือกข้าวโพดและเปลือกมะพร้าวในการช่วยเพิ่มปริม

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15

Fernando, Ilekuttige Priyan Shanura, Kalu Kapuge Asanka Sanjeewa, Hyo Geun Lee, et al. "Fucoidan Purified from Sargassum polycystum Induces Apoptosis through Mitochondria-Mediated Pathway in HL-60 and MCF-7 Cells." Marine Drugs 18, no. 4 (2020): 196. http://dx.doi.org/10.3390/md18040196.

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Fucoidans are biocompatible, heterogeneous, and fucose rich sulfated polysaccharides biosynthesized in brown algae, which are renowned for their broad-spectrum biofunctional properties. As a continuation of our preliminary screening studies, the present work was undertaken to extract polysaccharides from the edible brown algae Sargassum polycystum by a modified enzyme assisted extraction process using Celluclast, a food-grade cellulase, and to purify fucoidan by DEAE-cellulose anion exchange chromatography. The apoptotic and antiproliferative properties of the purified fucoidan (F5) were evalu

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16

LEONARSKI, EDUARDO, GIULIA VALAR MARTINI, KARINA CESCA, MARCOS FELLIPE DA SILVA, ROSANA GOLDBECK, and PATRÍCIA POLETTO. "ENZYMATIC UPCYCLING OF BACTERIAL CELLULOSE FROM KOMBUCHA TO OBTAIN CELLOBIOSE." Cellulose Chemistry and Technology 57, no. 1-2 (2023): 125–32. http://dx.doi.org/10.35812/cellulosechemtechnol.2023.57.13.

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"The production of kombucha generates bacterial cellulose (BC) as a by-product, which is usually discarded. However, BC can be a source of cellobiose, a disaccharide with prebiotic benefits. In this study, the yield of cellobiose released from BC collected from a medium-sized kombucha producer was evaluated by enzymatic hydrolysis using the commercial cocktail Celluclast 1.5 L. The BC was hydrolyzed at solid contents of 2, 3 and 4% (m/v), enzyme dosage of 2.2 U/g cellulose, pH 5, 50 °C, and 150 rpm for 72 h. Industrial BC was characterized by FTIR and XRD to confirm the presence of common BC c

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17

Radenkovs, Vitalijs, Karina Juhnevica-Radenkova, Jorens Kviesis, and Anda Valdovska. "An Environmentally Friendly Approach for the Release of Essential Fatty Acids from Cereal By-Products Using Cellulose-Degrading Enzymes." Biology 11, no. 5 (2022): 721. http://dx.doi.org/10.3390/biology11050721.

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The main intention of the present work was to investigate the ability of cellulose-degrading enzymes (C-DE) to release fatty acids (FAs) from complex matrices of cereal by-products during enzymatic hydrolysis (EH). For this purpose, three types of cereal bran (CB), i.e., wheat, rye, and oat, were used as lignocellulose substrates for three commercially available hydrolytic enzymes, i.e., Viscozyme L, Viscoferm, and Celluclast 1.5 L. The yield and composition of FAs after EH were assessed and compared with those obtained after either conventional Soxhlet extraction or after alkaline-assisted hy

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18

Song, Jun-Hui, Se Yeon Won, Byungdoo Hwang, et al. "In Vitro and In Vivo Antitumor Efficacy of Hizikia fusiforme Celluclast Extract against Bladder Cancer." Nutrients 12, no. 7 (2020): 2159. http://dx.doi.org/10.3390/nu12072159.

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Various physiological benefits have been linked to Hizikia fusiforme (HF), an edible brown seaweed. Here, fucose-containing sulfated polysaccharides were extracted from celluclast-processed HF (SPHF) and their antitumor efficacy against bladder cancer was evaluated in vitro and in vivo. SPHF possesses high sulfated polysaccharide and fucose contents and free radical scavenging activities compared to those of celluclast-processed HF extracts (CHF). SPHF inhibited bladder cancer EJ cell proliferation via G1-phase cell cycle arrest. This was due to the induction of p21WAF1 expression associated w

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19

Vo, Tan Phat, Nguyen Hong Nhung Duong, Thuy Han Phan, Thanh Phong Mai, and Dinh Quan Nguyen. "Optimized Cellulase-Hydrolyzed Deoiled Coconut Cake Powder as Wheat Flour Substitute in Cookies." Foods 11, no. 17 (2022): 2709. http://dx.doi.org/10.3390/foods11172709.

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Deoiled coconut cake powder (DCCP) was hydrolyzed to reduce the ratio of insoluble/soluble dietary fiber (RIS) by partially converting insoluble dietary fiber to soluble using Celluclast 1.5 L, a commercial cellulase preparation in citrate buffer medium. Firstly, the influence of citrate buffer amount, enzyme concentration, pH, and retention time on the enzymatic hydrolysis efficiency was investigated. Then, response surface methodology (RSM) was employed to optimize the process in which the insoluble and soluble dietary fiber contents were the responses. The results revealed that 10.3 g buffe

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Yong, Hwan-Ung, Seonmi Kim та Jae-Hoon Shim. "Enzymatic Production of Alkyl β-Glucoside Based on Transglycosylation Activity of Celluclast". Journal of the Korean Society of Food Science and Nutrition 41, № 10 (2012): 1417–22. http://dx.doi.org/10.3746/jkfn.2012.41.10.1417.

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21

Yuliarti, Oni, Lara Matia-Merino, Kelvin K. T. Goh, John A. Mawson, and Charles S. Brennan. "Effect of Celluclast 1.5L on the Physicochemical Characterization of Gold Kiwifruit Pectin." International Journal of Molecular Sciences 12, no. 10 (2011): 6407–17. http://dx.doi.org/10.3390/ijms12106407.

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22

Hjortkjaer, R. K., V. Bille-Hansen, K. P. Hazelden, et al. "Safety evaluation of celluclast®, an acid cellulase derived from Trichoderma reesei." Food and Chemical Toxicology 24, no. 1 (1986): 55–63. http://dx.doi.org/10.1016/0278-6915(86)90265-6.

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23

Wang, Lei, Jae Young Oh, Jin Hwang, Jae Young Ko, You-Jin Jeon, and BoMi Ryu. "In Vitro and In Vivo Antioxidant Activities of Polysaccharides Isolated from Celluclast-Assisted Extract of an Edible Brown Seaweed, Sargassum fulvellum." Antioxidants 8, no. 10 (2019): 493. http://dx.doi.org/10.3390/antiox8100493.

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It has been reported that enzymatic digestion of algae could improve the yield and enhance the biological activity compared to water and organic extraction. Our previous research indicated that Celluclast-assisted extract of Sargassum fulvellum (SF) possessed higher carbohydrate content and stronger antioxidant activity compared to water and other enzyme-assisted extracts. In the present study, we evaluated the antioxidant activities of polysaccharides from SF (SFPS) in vitro in Vero cells and in vivo in zebrafish. SFPS was obtained by Celluclast-assisted hydrolysis and ethanol precipitation.

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24

Ayuso, Pablo, Rocío Peñalver, Jhazmin Quizhpe, María de los Ángeles Rosell, and Gema Nieto. "Broccoli, Artichoke, Carob and Apple By-Products as a Source of Soluble Fiber: How It Can Be Affected by Enzymatic Treatment with Pectinex® Ultra SP-L, Viscozyme® L and Celluclast® 1.5 L." Foods 14, no. 1 (2024): 10. https://doi.org/10.3390/foods14010010.

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Dietary fiber (DF), and especially soluble dietary fiber (SDF), is a nutrient of particular interest today because of its anti-inflammatory role and its ability to reduce cardiovascular risk. Therefore, the enhancement of SDF in foods using different techniques has become a promising field of research. In order to prove the possibility of increasing this SDF content, the effects of different commercial enzymes (Pectinex® Ultra SP-L, Viscozyme® L and Celluclast® 1.5 L) were tested on a variety of carob (CE), artichoke (ARE), apple (APE) and broccoli (BE) by-product extracts. Enzymatic treatment

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Kairė, Aurelija, Jolita Jagelavičiūtė, Loreta Bašinskienė, Michail Syrpas, and Dalia Čižeikienė. "Influence of Enzymatic Hydrolysis on Composition and Technological Properties of Black Currant (Ribes nigrum) Pomace." Applied Sciences 15, no. 11 (2025): 6207. https://doi.org/10.3390/app15116207.

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Blackcurrant (Ribes nigrum) is valued for its health-promoting compounds, many of which remain in the pomace after juice extraction. Berry pomace can be considered a valuable source of dietary fiber. However, it is typically dominated by insoluble dietary fiber (IDF), and the soluble-to-insoluble fiber ratio is often nutritionally suboptimal. The aim of this study was to evaluate the influence of enzymatic hydrolysis on the composition and technological properties of blackcurrant pomace (BCP). Three commercial enzyme preparations—Viscozyme® L, Celluclast® 1.5 L, and Pectinex® Ultra Tropical (N

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de Gouvêa, Paula Fagundes, Luis Eduardo Gerolamo, Aline Vianna Bernardi, Lucas Matheus Soares Pereira, Sergio Akira Uyemura, and Taisa Magnani Dinamarco. "Lytic Polysaccharide Monooxygenase from Aspergillus fumigatus can Improve Enzymatic Cocktail Activity During Sugarcane Bagasse Hydrolysis." Protein & Peptide Letters 26, no. 5 (2019): 377–85. http://dx.doi.org/10.2174/0929866526666190228163629.

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Background: Lytic Polysaccharide Monooxygenases (LPMOs) are auxiliary accessory enzymes that act synergistically with cellulases and which are increasingly being used in secondgeneration bioethanol production from biomasses. Several LPMOs have been identified in various filamentous fungi, including Aspergillus fumigatus. However, many LPMOs have not been characterized yet. Objective: To report the role of uncharacterized A. fumigatus AfAA9_B LPMO. Methods: qRT-PCR analysis was employed to analyze the LPMO gene expression profile in different carbon sources. The gene encoding an AfAA9_B (Afu4g0

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De la Peña-Armada, Rocío, María José Villanueva-Suárez, Pilar Rupérez, and Inmaculada Mateos-Aparicio. "High Hydrostatic Pressure Assisted by Celluclast® Releases Oligosaccharides from Apple By-Product." Foods 9, no. 8 (2020): 1058. http://dx.doi.org/10.3390/foods9081058.

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A novel and green procedure consisting of high hydrostatic pressure (HHP) aided by a commercial cellulase (Celluclast®) has been applied to valorise the apple by-product, a valuable source of dietary fibre but mainly composed by insoluble fibre. Optimal conditions for solubilisation of dietary fibre were first determined at atmospheric pressure as 2% (w/v) of substrate concentration and 20 Endo-Glucanase Units of cellulase. Monitoring of polysaccharides and oligosaccharides released from apple by-product was carried out by means of a newly validated HPLC method with refractive index detector.

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Zhang, Wencan, Fangju Zhao, Tiankui Yang, Feifei Zhao, and Shaoquan Liu. "Celluclast 1.5L pretreatment enhanced aroma of palm kernels and oil after kernel roasting." Journal of the Science of Food and Agriculture 97, no. 15 (2017): 5146–57. http://dx.doi.org/10.1002/jsfa.8394.

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29

Dranca, Florina, and Mircea Oroian. "Optimization of Pectin Enzymatic Extraction from Malus domestica ‘Fălticeni’ Apple Pomace with Celluclast 1.5L." Molecules 24, no. 11 (2019): 2158. http://dx.doi.org/10.3390/molecules24112158.

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Pectin was extracted from apple (Malus domestica ‘Fălticeni’) pomace with Celluclast 1.5L, at doses of 20, 40, and 60 µL/g of material. The temperature and time of extraction were varied on three levels—temperature—40, 50, and 60 °C; time—12, 18, and 24 h. For each experiment, the extraction yield (R2 = 0.8905), the galacturonic acid content (R2 = 0.9866), and the degree of esterification (R2 = 0.9520) of pectin was determined. Response surface methodology (RSM) was implemented via a Box–Behnken design, to optimize pectin extraction. In the optimum extraction conditions (temperature of 48.3 °C

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Šimkutė, Simona, Loreta Bašinskienė, Michail Syrpas, and Dalia Čižeikienė. "Composition and Technological Properties of Modified Lingonberry (Vaccinium vitis-idaea L.) Pomace." Applied Sciences 15, no. 7 (2025): 3661. https://doi.org/10.3390/app15073661.

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Lingonberry pomace (LP) is a by-product rich in valuable bioactive compounds and can be used in the food industry after various treatments and property characterization. This study aimed to evaluate the impact of commercially available enzymes (Viscozyme® L, Pectinex® Ultra Tropical, and Celluclast® 1.5 L) and supercritical carbon dioxide (SFE-CO2) extraction technology on the chemical composition and technological properties of treated LP products. The Megazyme kit was used to determine the soluble dietary fiber (SDS) and insoluble dietary fiber (IDF) contents, while the changes in mono-, dis

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Jin, Xian Chun, An Dong Song, Tong Fu Su, and Bia Liang Zhang. "Effect of Detoxification of Natural Drying Steam Exploded Corn Straw with Organic Solvent on Enzymatic Hydrolysis and Ethanol Fermentation." Advanced Materials Research 512-515 (May 2012): 379–83. http://dx.doi.org/10.4028/www.scientific.net/amr.512-515.379.

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Corn straw from steam-explosion treatment process was treated with different organic solvents to reduce the inhibition for the sequent enzymatic hydrolysis and fermentation. The detoxiﬁed substrates (100 g DW/L) were hydrolyzed with a blend of Novozym 188 (15 IU/g cellulose), Celluclast CP cellulase (15 FPU/g cellulose) and lignase (15 IU/g hemicellulose). The reducing sugar was bio-converted to ethanol with separated hydrolysis and fermentation processes using Pachysolen tannophilus P-01. The reducing sugar yield varied from 34.8 to 89.7% depending on the detoxiﬁcation solvents and modes. The

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Sabater, Carlos, Nieves Corzo, Agustín Olano, and Antonia Montilla. "Enzymatic extraction of pectin from artichoke (Cynara scolymus L.) by-products using Celluclast®1.5L." Carbohydrate Polymers 190 (June 2018): 43–49. http://dx.doi.org/10.1016/j.carbpol.2018.02.055.

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Du, Min, Xin Ping Li, and Zhi Min Yao. "Influence of Cellulase to Aggregation Structure of Bleached Softwood Pulp." Advanced Materials Research 549 (July 2012): 255–59. http://dx.doi.org/10.4028/www.scientific.net/amr.549.255.

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Cellulase named as Celluclast 1.5L was used to deal with bleached softwood pulp. By analyzing the changes of water retention value and crystallinity of fiber after enzymatic treatment, studied the influence of cellulase to aggregation structure of bleached softwood pulp. The results show that with the increasing of the enzyme dosage, the proportion of long-fiber decreases and the content of fines increases. Water retention value of pulp first decreases and then increases. The fiber crystal does not change with the enzymatic treatment, but with the increasing of the enzyme dosage, the crystalli

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Du, Min, Xin Ping Li, and Meng Luo. "Influence of Cellulase to Filtration and Fiber Morphology of Bleached Softwood Pulp." Advanced Materials Research 550-553 (July 2012): 1182–85. http://dx.doi.org/10.4028/www.scientific.net/amr.550-553.1182.

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Cellulase named as Celluclast 1.5L was used to deal with bleached softwood pulp. By analyzing the changes of fiber polymerization, fiber length and pulp freeness before and after enzymatic treatment, studied the influence of cellulase to filtration and fiber morphology of bleached softwood pulp, and discussed the mode of action of cellulase to bleached softwood pulp. The results show that while the enzyme dosage increasing, the degree of cellulose hydrolysis increases; the pulp polymerization degree decreases; fiber length decreases and the content of fines increase. Pulp filtration first incr

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Karnaouri, Anthi, Leonidas Matsakas, Saskja Bühler, Madhu Nair Muraleedharan, Paul Christakopoulos, and Ulrika Rova. "Tailoring Celluclast® Cocktail’s Performance towards the Production of Prebiotic Cello-Oligosaccharides from Waste Forest Biomass." Catalysts 9, no. 11 (2019): 897. http://dx.doi.org/10.3390/catal9110897.

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The main objective of this study focused on the sustainable production of cellobiose and other cellulose-derived oligosaccharides from non-edible sources, more specifically, from forest residues. For this purpose, a fine-tuning of the performance of the commercially available enzyme mixture Celluclast® was conducted towards the optimization of cellobiose production. By enzyme reaction engineering (pH, multi-stage hydrolysis with buffer exchange, addition of β-glucosidase inhibitor), a cellobiose-rich product with a high cellobiose to glucose ratio (37.4) was achieved by utilizing organosolv-pr

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Tang, S., N. S. Hettiarachchy, S. Eswaranandam, and P. Crandall. "Protein Extraction from Heat-stabilized Defatted Rice Bran: II. The Role of Amylase, Celluclast, and Viscozyme." Journal of Food Science 68, no. 2 (2003): 471–75. http://dx.doi.org/10.1111/j.1365-2621.2003.tb05696.x.

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Park, Eun-Young, and Jung-Kyu Park. "Sequential Hydrothermal HCl Pretreatment and Enzymatic Hydrolysis of Saccharina japonica Biomass." Energies 14, no. 23 (2021): 8053. http://dx.doi.org/10.3390/en14238053.

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This study investigated the production of fermentable sugars from carbohydrate-rich macroalgae Saccharina japonica using sequential hydrolysis (hydrothermal acid pretreatment and enzymatic hydrolysis) to determine the maximum reducing sugar yield (RSy). The sequential hydrolysis was predicted by three independent variables (temperature, time, and HCl concentration) using response surface methodology (RSM). Enzymatic hydrolysis (8.17% v/wbiomass Celluclast® 1.5 L, 26.4 h, 42.6 °C) was performed after hydrothermal acid pretreatment under predicted conditions (143.6 °C, 22 min, and 0.108 N HCl co

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Gabiatti Junior, Claudio, Lucas Dal Magro, Natália G. Graebin, Eliseu Rodrigues, Rafael C. Rodrigues, and Carlos Prentice. "Combination of Celluclast and Viscozyme improves enzymatic hydrolysis of residual cellulose casings: process optimization and scale-up." Brazilian Journal of Chemical Engineering 37, no. 3 (2020): 463–73. http://dx.doi.org/10.1007/s43153-020-00050-w.

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Yusof, Nurhayati. "Production of Chitosan Oligosaccharides using β-glycosidic degrading enzyme: Optimization using Response Surface Methodology". Malaysian Journal of Applied Sciences 5, № 2 (2020): 30–44. http://dx.doi.org/10.37231/myjas.2020.5.2.261.

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Many researchers have focused chitosan as a source of potential bioactive material during the past few decades. However, chitosan has several drawbacks to be utilised in biological applications, including poor solubility under physiological conditions. Therefore, a new interest has recently emerged on partially hydrolysed chitosan, chitosan oligosaccharides (COS). In this study, degradation of chitosan was performed by Cellulase from Trichoderma reesei® 1.5L and Response Surface Methodology (RSM) were employed to optimize the hydrolysis temperature, pH, enzyme concentration and substrate conce

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Hennessey-Ramos, Licelander, Walter Murillo-Arango, Juliana Vasco-Correa, and Isabel Cristina Paz Astudillo. "Enzymatic Extraction and Characterization of Pectin from Cocoa Pod Husks (Theobroma cacao L.) Using Celluclast® 1.5 L." Molecules 26, no. 5 (2021): 1473. http://dx.doi.org/10.3390/molecules26051473.

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Cocoa pod husks are a waste generated during the processing of cocoa beans. We aimed to explore the enzymatic extraction of pectin using cellulases. The extraction process was optimized using a central composite design (CCD) and analyzed by response surface methodology (RSM). The parameters optimized were feedstock concentration (%), enzyme dosage (µL/g), and time (h). Three dependent variables were studied: pectin yield (g/100 g dry husk) (R2 = 97.02), galacturonic acid content (g/100 g pectin) (R2 = 96.90), and galacturonic acid yield (g/100 g feedstock) (R2 = 95.35). The optimal parameters

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Poyraz, Bayram, Ayhan Tozluoğlu, Zeki Candan, Ahmet Demir, and Mustafa Yavuz. "Influence of PVA and silica on chemical, thermo-mechanical and electrical properties of Celluclast-treated nanofibrillated cellulose composites." International Journal of Biological Macromolecules 104 (November 2017): 384–92. http://dx.doi.org/10.1016/j.ijbiomac.2017.06.018.

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Méndez-Líter, Juan A., Laura I. de Eugenio, Neumara L. S. Hakalin, Alicia Prieto та María Jesús Martínez. "Production of a β-Glucosidase-Rich Cocktail from Talaromyces amestolkiae Using Raw Glycerol: Its Role for Lignocellulose Waste Valorization". Journal of Fungi 7, № 5 (2021): 363. http://dx.doi.org/10.3390/jof7050363.

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As β-glucosidases represent the major bottleneck for the industrial degradation of plant biomass, great efforts are being devoted to discover both novel and robust versions of these enzymes, as well as to develop efficient and inexpensive ways to produce them. In this work, raw glycerol from chemical production of biodiesel was tested as carbon source for the fungus Talaromyces amestolkiae with the aim of producing enzyme β-glucosidase-enriched cocktails. Approximately 11 U/mL β-glucosidase was detected in these cultures, constituting the major cellulolytic activity. Proteomic analysis showed

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Nguyen, Lien Le Phuong, and Man Van Viet Le. "APPICATION OF COMMERCIAL ENZYMES FOR JICAMA PULP TREATMENT IN JUICE PRODUCTION." Science and Technology Development Journal 13, no. 1 (2010): 64–76. http://dx.doi.org/10.32508/stdj.v13i1.2071.

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Jicama or Yam bean (Pachyrhizus erosus) tuber is a popular tropical legume in Viet Nam. It contains glucids, different amino acids, vitamins and minerals. Until present, jicama has not been used as a principal raw material in food industry in our country. Enzymatic treatment of raw material is a well-known technique for increasing the extraction yield in fruit and vegetable processing. The aim of this work is to investigate effects of enzymatic treatment of jicama pulp on the extraction yield, and sugar content in the jicama juice production. The highest extraction yield was 92,7% when jicama

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Oh, Jae-Young, Eun-A. Kim, Sang In Kang, et al. "Protective Effects of Fucoidan Isolated from Celluclast-Assisted Extract of Undaria pinnatifida Sporophylls against AAPH-Induced Oxidative Stress In Vitro and In Vivo Zebrafish Model." Molecules 25, no. 10 (2020): 2361. http://dx.doi.org/10.3390/molecules25102361.

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Fucoidan is a fucose-enriched polysaccharide, obtained from brown algae, with demonstrated antioxidant properties. However, traditional extraction methods using water or chemical-based extraction methods have reduced yield and produced hazardous by-products. In this study, we isolated fucoidan at a high yield using enzyme-assisted extraction; the Celluclast enzyme assisted extract of Undaria pinnatifida sporophylls (FCUS). To examine the antioxidant properties of FCUS, oxidative stress was induced with 2,2′-azobis (2-methylpropionamidine) dihydrochloride (AAPH) in Vero cells and zebrafish mode

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Gutöhrlein, Friederike, Stephan Drusch, and Sebastian Schalow. "Towards By-Product Utilisation of Pea Hulls: Isolation and Quantification of Galacturonic Acid." Foods 7, no. 12 (2018): 203. http://dx.doi.org/10.3390/foods7120203.

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In order to evaluate by-products from food processing as alternative raw materials for pectin extraction, their amount of galacturonic acid (GalA) has to be analysed as a marker for pectin content. In the present study, significant differences in GalA release using different digestion methods are shown for pea hulls, as an example of by-products with a high content of cellulose. Complete digestion of the fibre matrix was assumed for Saeman hydrolysis as a reference protocol. Significantly lower GalA release was achieved by a treatment with trifluoracetic acid (TFA). An alternative treatment wi

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Sriariyanun, Malinee, Nichaphat Kitiborwornkul, Prapakorn Tantayotai, Kittipong Rattanaporn, and Pau-Loke Show. "One-Pot Ionic Liquid-Mediated Bioprocess for Pretreatment and Enzymatic Hydrolysis of Rice Straw with Ionic Liquid-Tolerance Bacterial Cellulase." Bioengineering 9, no. 1 (2022): 17. http://dx.doi.org/10.3390/bioengineering9010017.

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Ionic liquid (IL) pretreatment of lignocellulose is an efficient method for the enhancement of enzymatic saccharification. However, the remaining residues of ILs deactivate cellulase, therefore making intensive biomass washing after pretreatment necessary. This study aimed to develop the one-pot process combining IL pretreatment and enzymatic saccharification by using low-toxic choline acetate ([Ch][OAc]) and IL-tolerant bacterial cellulases. Crude cellulases produced from saline soil inhabited Bacillus sp. CBD2 and Brevibacillus sp. CBD3 were tested under the influence of 0.5–2.0 M [Ch][OAc],

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Wang, Lei, WonWoo Lee, Jae Oh, Yong Cui, BoMi Ryu та You-Jin Jeon. "Protective Effect of Sulfated Polysaccharides from Celluclast-Assisted Extract of Hizikia fusiforme Against Ultraviolet B-Induced Skin Damage by Regulating NF-κB, AP-1, and MAPKs Signaling Pathways In Vitro in Human Dermal Fibroblasts". Marine Drugs 16, № 7 (2018): 239. http://dx.doi.org/10.3390/md16070239.

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Our previous study evaluated the antioxidant activities of sulfated polysaccharides from Celluclast-assisted extract of Hizikia fusiforme (HFPS) in vitro in Vero cells and in vivo in zebrafish. The results showed that HFPS possesses strong antioxidant activity and suggested the potential photo-protective activities of HFPS. Hence, in the present study, we investigated the protective effects of HFPS against ultraviolet (UV) B-induced skin damage in vitro in human dermal fibroblasts (HDF cells). The results indicate that HFPS significantly reduced intracellular reactive oxygen species (ROS) leve

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Jayawardena, Thilina U., Lei Wang, K. K. Asanka Sanjeewa, Sang In Kang, Jung-Suck Lee, and You-Jin Jeon. "Antioxidant Potential of Sulfated Polysaccharides from Padina boryana; Protective Effect against Oxidative Stress in In Vitro and In Vivo Zebrafish Model." Marine Drugs 18, no. 4 (2020): 212. http://dx.doi.org/10.3390/md18040212.

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Elevated levels of reactive oxygen species (ROS) damage the internal cell components. Padina boryana, a brown alga from the Maldives, was subjected to polysaccharide extraction. The Celluclast enzyme assisted extract (PBE) and ethanol precipitation (PBP) of P. boryana were assessed against hydrogen peroxide (H2O2) induced cell damage and zebra fish models. PBP which contains the majority of sulfated polysaccharides based on fucoidan, showed outstanding extracellular ROS scavenging potential against H2O2. PBP significantly declined the intracellular ROS levels, and exhibited protection against

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Im, H. J., and K. Y. Yoon. "Production and characterisation of alcohol-insoluble dietary fibre as a potential sourcefor functional carbohydrates produced by enzymatic depolymerisation of buckwheat hulls." Czech Journal of Food Sciences 33, No. 5 (2016): 449–57. http://dx.doi.org/10.17221/200/2015-cjfs.

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We examined the potential use of buckwheat hulls as a raw material for producing soluble dietary fibre. The insoluble fibre fraction obtained from buckwheat hulls was hydrolysed by two commercial enzymes (Celluclast 1.5L for the cellulose fraction and Viscozyme L for the hemicellulose fraction) to obtain soluble fibre hydrolysates. Alcohol-insoluble dietary fibre (AIF) was separated from the freeze-dried soluble hydrolysate by treatment with 85% ethanol. The water-holding, oil-binding, and swelling capacities of AIF were increased by enzymatic hydrolysis. AIF had significantly (P&nbsp;&amp

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Karim, Latiffah, and Shikh Mohd Shahrul Nizan Shikh Zahari. "Stability of cellulases in ionic liquids." Malaysian Journal of Fundamental and Applied Sciences 15, no. 3 (2019): 432–35. http://dx.doi.org/10.11113/mjfas.v15n3.1297.

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Lignocellulosic biomass has been used as an alternative source to food crops that serve as feedstock for bioenergy production. The conversion of biomass to bioenergy required pretreatment process. Ionic liquids (ILs) have been recognized as promising solvents that are capable of solubilizing and separating components of lignocellulosic biomass. This research focuses on understanding how ILs affects the activity of cellulases in the enzymatic saccharification process. Sigmacell cellulose was used in the enzymatic saccharification process inste. Two different ILs were added in the enzymatic sacc

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