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Journal articles on the topic 'Cellular fibers'

Author: Grafiati

Published: 30 May 2022

Last updated: 31 July 2025

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1

Powers, S. K., D. Criswell, F. K. Lieu, S. Dodd, and H. Silverman. "Exercise-induced cellular alterations in the diaphragm." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 263, no. 5 (1992): R1093—R1098. http://dx.doi.org/10.1152/ajpregu.1992.263.5.r1093.

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Limited data exist concerning the effects of exercise training on cellular oxidative capacity in the diaphragm of senescent animals. In this study we examined the changes in cellular oxidative capacity, muscle cell cross-sectional area (CSA), and capillarity within the costal diaphragm of senescent animals after a 10-wk endurance-training program. Twelve 24-mo-old female Fischer 344 rats were divided into either a sedentary control group (n = 6) or exercise training group (n = 6). The trained animals exercised on a motor-driven treadmill (60 min/day, 5 days/wk) at a work rate equal to approxim

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2

Huang, Hu, Feihao Chen, Kelei Cao, Xiancai Zhang, and Ruihang Li. "Damage Evolution Characteristics of Steel-Fiber-Reinforced Cellular Concrete Based on Acoustic Emission." Buildings 15, no. 2 (2025): 229. https://doi.org/10.3390/buildings15020229.

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In order to investigate the steel fiber parameters on the damage characteristics and crack evolution of cellular concrete materials, uniaxial compression–acoustic emission combined tests were carried out on steel-fiber-reinforced cellular concrete (SFRCC) with different steel fiber contents (0%, 0.5%, 1%, 1.5%, and 2%) and different porosities (10% and 20%). The material damage evolution characteristics were analyzed by acoustic emission parameters and IB values, and the crack types were identified using Gaussian mixture clustering method (GMM) pairs. The results show the following: the inclus

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3

Netsvet, Daria Dmitrievna, Alexandr L. Popov, Viktoriya Viktorovna Nelubova, and Svetlana V. Lasunova. "Properties of Microfibers of Various Compositions as a Component of Cellular Composites." Materials Science Forum 1040 (July 27, 2021): 132–38. http://dx.doi.org/10.4028/www.scientific.net/msf.1040.132.

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The paper presents studies on the properties of various types of micro-reinforcing fibers to assess their role and effectiveness in the structure formation of the cellular composite. Based on the data on the weight loss after exposure in a model medium of cement, analysis of the alkali resistance of fibers of five different types – basalt fiber, heat-treated basalt fiber, polymer fiber and glass fibers from two different manufacturers – was carried out. It is shown that the fibers have a sufficiently high durability in the medium of hardening cement, which is expressed by a relatively insignif

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Hardy, Kristin M., Richard M. Dillaman, Bruce R. Locke, and Stephen T. Kinsey. "A skeletal muscle model of extreme hypertrophic growth reveals the influence of diffusion on cellular design." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 296, no. 6 (2009): R1855—R1867. http://dx.doi.org/10.1152/ajpregu.00076.2009.

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Muscle fibers that power swimming in the blue crab Callinectes sapidus are <80 μm in diameter in juveniles but grow hypertrophically, exceeding 600 μm in adults. Therefore, intracellular diffusion distances become progressively greater as the animals grow and, in adults, vastly exceed those in most cells. This developmental trajectory makes C. sapidus an excellent model for characterization of the influence of diffusion on fiber structure. The anaerobic light fibers, which power burst swimming, undergo a prominent shift in organelle distribution with growth. Mitochondria, which require O2 a

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5

Luden, Nicholas, Kiril Minchev, Erik Hayes, Emily Louis, Todd Trappe, and Scott Trappe. "Human vastus lateralis and soleus muscles display divergent cellular contractile properties." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 295, no. 5 (2008): R1593—R1598. http://dx.doi.org/10.1152/ajpregu.90564.2008.

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The purpose of this study was to investigate potential differences in single-fiber contractile physiology of fibers with the same myosin heavy chain isoform (MHC I and MHC IIa) originating from different muscles. Vastus lateralis (VL) and soleus biopsies were obtained from 27 recreationally active females (31 ± 1 yr, 59 ± 1 kg). A total of 943 single fibers (MHC I = 562; MHC IIa = 301) were isolated and examined for diameter, peak tension (Po), shortening velocity (Vo), and power. The soleus had larger ( P < 0.05) fibers (MHC I +18%; MHC IIa +19%), higher MHC I Vo (+13%), and higher MHC I P

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Baranova, A. A. "CELLULAR AND FINE-GRAINED CONCRETE BASED ON SILICA WITH DISPERSED REINFORCEMENT." Mechanics and Technologies, no. 4 (December 30, 2023): 83–91. http://dx.doi.org/10.55956/qmmz3329.

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The results of studies of the physical and mechanical properties of heat-efficient foam concrete, as well as fine-grained concrete, are summarized. The materials are made on the basis of silica microfibre. The percentage of fibers varies within the prescribed limits, and they must be prepared from different materials. The determination of physical and mechanical properties corresponds to GOST 180-2012. Thus, it was found that the optimal content of basalt and steel fiber fibers in foam concrete samples is about – 2%, polypropylene – 3%. The compressive strength of foam concrete samples increas

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7

Gao, Xiang, Jingjun Peng, Linjie Huang, et al. "Electrospun Parallel, Crossed Fibers for Promoting Cell Adhesion and Migration." Materials 18, no. 14 (2025): 3224. https://doi.org/10.3390/ma18143224.

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Electrospun fibers, possessing biomimetic characteristics similar to fibrous extracellular matrices, have attracted widespread attention as scaffold materials for skin tissue engineering. The topographical structure of electrospun fibers plays a critical role in determining cell behavior. However, the effects of fiber topography on human skin fibroblasts (HSFs) remain unclear. In this study, electrospinning technology was employed to investigate how parallel and crossed fiber architectures influence the spreading morphology, proliferation, and migration of HSFs. The results demonstrated that c

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8

Hassan Nensok, Mohammed, Md Azree Othuman Mydin, and Hanizam Awang. "Optimization of mechanical properties of cellular lightweight concrete with alkali treated banana fiber." Revista de la construcción 20, no. 3 (2021): 491–511. http://dx.doi.org/10.7764/rdlc.20.3.491.

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Recent advancements in construction materials development have involved the utilization of plant-based natural fibers such as kenaf, sisal, coir and banana to replace conventional fibers such as carbon, steel, polypropylene and aramid. However, the main issue with using these fibers is the alkaline cement matrix's durability and compatibility due to high water absorption. Hence, this research focuses on the use of alkali treatment of banana fibers to enhance the mechanical properties of cellular lightweight concrete (CLC). Banana fibers were subjected to 2%, 4%, 6%, 8%, and 10% NaOH treatment

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9

Jung, Soo Yeon. "Aging Larynx: Molecular and Cellular Aspect." Journal of The Korean Society of Laryngology, Phoniatrics and Logopedics 35, no. 1 (2024): 6–9. http://dx.doi.org/10.22469/jkslp.2024.35.1.6.

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In South Korea, the elderly population is rapidly growing, projected to reach 20.6% by 2025. With increased social engagement among older individuals, the demand for voice-related interventions rises. Age-related changes in these structures impact vocal function and quality. Understanding the mechanisms underlying these changes is crucial for developing effective therapeutic approaches. The aging of the larynx occurs in each part of the cartilage, muscles, and vocal folds. The change in cartilage is most prominently characterized by ossification, which begins in the twenties and accelerates si

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10

Larkins, Noni T., Robyn M. Murphy та Graham D. Lamb. "Absolute amounts and diffusibility of HSP72, HSP25, and αB-crystallin in fast- and slow-twitch skeletal muscle fibers of rat". American Journal of Physiology-Cell Physiology 302, № 1 (2012): C228—C239. http://dx.doi.org/10.1152/ajpcell.00266.2011.

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Heat shock proteins (HSPs) are essential for normal cellular stress responses. Absolute amounts of HSP72, HSP25, and αB-crystallin in rat extensor digitorum longus (EDL) and soleus (SOL) muscle were ascertained by quantitative Western blotting to better understand their respective capabilities and limitations. HSP72 content of EDL and SOL muscle was only ∼1.1 and 4.6 μmol/kg wet wt, respectively, and HSP25 content approximately twofold greater (∼3.4 and ∼8.9 μmol/kg, respectively). αB-crystallin content of EDL muscle was ∼4.9 μmol/kg but in SOL muscle was ∼30-fold higher (∼140 μmol/kg). To exa

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11

Straight, Chad R., Olivia R. Ringham, Jenna M. Bartley, et al. "Influenza Infection has Fiber Type-Specific Effects on Cellular and Molecular Skeletal Muscle Function in Aged Mice." Journals of Gerontology: Series A 75, no. 12 (2020): 2333–41. http://dx.doi.org/10.1093/gerona/glaa136.

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Abstract Skeletal muscle myopathies represent a common non-pulmonary manifestation of influenza infection, leading to reduced physical function and hospitalization in older adults. However, underlying mechanisms remain poorly understood. Our study examined the effects of influenza virus A pulmonary infection on contractile function at the cellular (single fiber) and molecular (myosin-actin interactions and myofilament properties) levels in soleus and extensor digitorum longus muscles of aged (20 months) C57BL/6 male mice that were healthy or flu-infected for 7 (7-days post-infection; 7-DPI) or

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12

Ramos, Letícia Maria Alves, João Vicente de Figueiredo Latorraca, Thayanne Caroline Castor Neto, Letícia Souza Martins, and Elias Taylor Durgante Severo. "ANATOMICAL CHARACTERIZATION OF TENSION WOOD IN Hevea brasiliensis (Willd. ex A. Juss.) Mull. Arg." Revista Árvore 40, no. 6 (2016): 1099–107. http://dx.doi.org/10.1590/0100-67622016000600016.

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ABSTRACT Tension wood is an important anatomical structure for its participation in the orientation of the trunk and the architecture of the branches as a function of structural reinforcement. However, its presence in large amounts significantly affects the technological properties of wood, just as in the rubber tree. Nevertheless, there is still demand for information about the origin, distribution and structural features in this species. Thus, this study aims to characterize the cellular structures in tension and opposite wood in Hevea brasiliensis (rubber tree), as well as its radial and lo

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13

Ba, X., Y. Meng, Y. Huang, et al. "In Vitro Biomineralization Induced by Self-Assembled Extracellular Matrix Proteins." Key Engineering Materials 361-363 (November 2007): 427–30. http://dx.doi.org/10.4028/www.scientific.net/kem.361-363.427.

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Extracellular matrix (ECM) proteins play an essential role during biomineralization in bone and engineered tissues. In a previous study [1], we showed that calcite preferentially nucleated on pure elastin fibers. However, the actual cellular ECM fibers are composed of a combination of proteins, primarily collagen, fibronectin and some elastin. Here we follow the calcium carbonate- and calcium phosphate- mineralization process in vitro when these ECM proteins are combined and determine the differences between these proteins in the biomineralization process. The surface morphology and mechanical

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14

Rajan, Anandi, Elin Palm, Fredrik Trulsson, et al. "Heparan Sulfate Is a Cellular Receptor for Enteric Human Adenoviruses." Viruses 13, no. 2 (2021): 298. http://dx.doi.org/10.3390/v13020298.

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Human adenovirus (HAdV)-F40 and -F41 are leading causes of diarrhea and diarrhea-associated mortality in children under the age of five, but the mechanisms by which they infect host cells are poorly understood. HAdVs initiate infection through interactions between the knob domain of the fiber capsid protein and host cell receptors. Unlike most other HAdVs, HAdV-F40 and -F41 possess two different fiber proteins—a long fiber and a short fiber. Whereas the long fiber binds to the Coxsackievirus and adenovirus receptor (CAR), no binding partners have been identified for the short fiber. In this st

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15

Biring, Manmohan S., Mario Fournier, David J. Ross, and Michael I. Lewis. "Cellular adaptations of skeletal muscles to cyclosporine." Journal of Applied Physiology 84, no. 6 (1998): 1967–75. http://dx.doi.org/10.1152/jappl.1998.84.6.1967.

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The aim of this study was to evaluate the cellular response of the diaphragm, extensor digitorum longus (EDL), and soleus (Sol) muscles to clinically relevant doses of cyclosporine administered to male rats over 4 wk. Control rats were provided with vehicle only. Muscle fiber types, cross-sectional areas, indexes of capillarity, and succinate dehydrogenase (SDH) activity were determined by quantitative histochemistry. Myosin heavy chain isoforms were identified by SDS-PAGE, and their proportions were measured by scanning densitometry. Serum cyclosporine level, 20–24 h after the last dose of cy

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16

Carey, Shawn P., Zachary E. Goldblatt, Karen E. Martin, Bethsabe Romero, Rebecca M. Williams, and Cynthia A. Reinhart-King. "Local extracellular matrix alignment directs cellular protrusion dynamics and migration through Rac1 and FAK." Integrative Biology 8, no. 8 (2016): 821–35. http://dx.doi.org/10.1039/c6ib00030d.

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Fiber alignment within tumor-mimetic engineered collagen matrices drives FAK- and Rac1-dependent cellular anisotropy that promotes protrusions along fibers and suppresses off-axis protrusions to direct cell migration.

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17

Lin, Ling, Yun Neng Chen, Wen Zhong Gong, and Shan Yuan Wang. "Antibacterial Efficiency and Cellular Toxicity of PET-Based Hollow Fibers Containing Silver Particles." Advanced Materials Research 441 (January 2012): 279–83. http://dx.doi.org/10.4028/www.scientific.net/amr.441.279.

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This work focuses on antibacterial efficacy and cellular toxicity of PET-based hollow fiber with silver particles incorporated (Ag/PET hollow fiber), which was synthesized by differential pressure method. Escherichia coli (E. coli) were used to investigate the antibacterial capability of Ag/PET hollow fiber with antibacterial kinetics experiments. The antibacterial results demonstrated that Ag/PET hollow fiber had an excellent antibacterial property against E. coli and the efficacy was dependent on several aspects including fiber length, weight and silver content. The cytotoxicity of Ag/PET ho

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18

Efimov, Boris, Sergey Isachenko, Mukhamad-Basir Kodzoev, Gulzar Dosanova, and Ekaterina Bobrova. "Dispersed reinforcement in concrete technology." E3S Web of Conferences 110 (2019): 01032. http://dx.doi.org/10.1051/e3sconf/201911001032.

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The methods of disperse reinforcement of a concrete matrix with the help of fibers make it possible to manufacture structures of complex configuration and solve problems of frost resistance of products. Fiber replaces secondary reinforcement, reducing the volume of use of structural steel reinforcement. Cellular fibrous concrete is characterized by high performance properties, especially increased strength in bending and stretching, impact strength and crack resistance. As a reinforcing component, it is preferable to use mineral fibers or thin basalt fiber. The use of polyamide fiber is allowe

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19

Kodzoev, M.-B. "Cellular concrete, reinforced with mineral fibers." Bulletin of Science and Practice, no. 10 (October 14, 2017): 134–37. https://doi.org/10.5281/zenodo.1012263.

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The Domestic building complex decides now three main tasks: the overcoming of consequences of the crisis, the development of the segment of economy–class and the promotion of advanced energy efficient technologies, which create a minimum environmental load. The most widely used in construction got insulating concrete based on lightweight aggregates, such as cellular concrete, foam concrete and aerated concrete. The economic crisis in 2008–2009 caused a decline in the production of most building materials (cement, concrete products and reinforced concrete structures, brick and other materials)

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20

Fournier, Mario, and Michael I. Lewis. "Functional, cellular, and biochemical adaptations to elastase-induced emphysema in hamster medial scalene." Journal of Applied Physiology 88, no. 4 (2000): 1327–37. http://dx.doi.org/10.1152/jappl.2000.88.4.1327.

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The scalene has been reported to be an accessory inspiratory muscle in the hamster. We hypothesize that with the chronic loads and/or dynamic hyperinflation associated with emphysema (Emp), the scalene will be actively recruited, resulting in functional, cellular, and biochemical adaptations. Emp was induced in adult hamsters. Inspiratory electromyogram (EMG) activity was recorded from the medial scalene and costal diaphragm. Isometric contractile and fatigue properties were evaluated in vitro. Muscle fibers were classified histochemically and immunohistochemically. Individual fiber cross-sect

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21

Saito, Kengo, Keishi Mizuguchi, Toshihide Horiike, Tung Anh Dinh Duong, Yohei Shinmyo, and Hiroshi Kawasaki. "Characterization of the Inner and Outer Fiber Layers in the Developing Cerebral Cortex of Gyrencephalic Ferrets." Cerebral Cortex 29, no. 10 (2018): 4303–11. http://dx.doi.org/10.1093/cercor/bhy312.

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Abstract Changes in the cerebral cortex of mammals during evolution have been of great interest. Ferrets, monkeys, and humans have more developed cerebral cortices compared with mice. Although the features of progenitors in the developing cortices of these animals have been intensively investigated, those of the fiber layers are still largely elusive. By taking the advantage of our in utero electroporation technique for ferrets, here we systematically investigated the cellular origins and projection patterns of axonal fibers in the developing ferret cortex. We found that ferrets have 2 fiber l

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22

Fournier, Mario, and Michael I. Lewis. "Influences of IGF-I gene disruption on the cellular profile of the diaphragm." American Journal of Physiology-Endocrinology and Metabolism 278, no. 4 (2000): E707—E715. http://dx.doi.org/10.1152/ajpendo.2000.278.4.e707.

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The impact of a targeted disruption of the Igf1 gene, encoding the insulin-like growth factor I (IGF-I), on diaphragm (DIA) cellularity was studied in 2-mo-old homozygous mutant [IGF-I(−/−)] mice and their wild-type [WT; i.e., IGF-I(+/+)] littermates. DIA fiber types were classified histochemically. DIA fiber cross-sectional areas (CSA) were determined from digitized muscle sections, and fiber succinate dehydrogenase (SDH) activity was determined histochemically using a microdensitometric procedure. An acidic ATPase reaction was used to visualize capillaries. Myosin heavy chain (MyHC) isoforms

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23

Kato, Yasushi P., Michael G. Dunn, Frederick H. Silver, and Arthur J. Wasserman. "Biological and biomedical applications of collagenous biomaterials." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 3 (1990): 856–57. http://dx.doi.org/10.1017/s0424820100161849.

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Collagenous biomaterials have been used for growing cells in vitro as well as for augmentation and replacement of hard and soft tissues. The substratum used for culturing cells is implicated in the modulation of phenotypic cellular expression, cellular orientation and adhesion. Collagen may have a strong influence on these cellular parameters when used as a substrate in vitro. Clinically, collagen has many applications to wound healing including, skin and bone substitution, tendon, ligament, and nerve replacement. In this report we demonstrate two uses of collagen. First as a fiber to support

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24

Chien, Hsi Hsin, Kung Jeng Ma, Yun Peng Yeh, and Choung Lii Chao. "Microstructure and Mechanical Properties of Air Core Polymer Photonic Crystal Fibers." Advanced Materials Research 233-235 (May 2011): 3000–3004. http://dx.doi.org/10.4028/www.scientific.net/amr.233-235.3000.

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Polymer based photonic crystal fibers with low cost manufacturability, and the mechanical and chemical flexibility offer key advantages over traditional silica based photonic crystal fibers. PMMA photonic crystal fiber was fabricated by stacking an array of PMMA capillaries to form a preform, and followed by fusing and drawing into fiber with a draw tower. The air hole diameter and fraction of photonic crystal fiber can be manipulated by the thickness of PMMA capillaries and drawing temperature. The measurement of mechanical properties was performed by universal testing machine. The air core g

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Drenckhahn, D., and J. Wagner. "Stress fibers in the splenic sinus endothelium in situ: molecular structure, relationship to the extracellular matrix, and contractility." Journal of Cell Biology 102, no. 5 (1986): 1738–47. http://dx.doi.org/10.1083/jcb.102.5.1738.

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In the present study, we investigated structural and functional aspects of stress fibers in a cell type in situ, i.e., the sinus endothelium of the human spleen. In this cell type, stress fibers extend underneath the basal plasma membrane and are arranged parallel to the cellular long axis. Ultrastructurally, the stress fibers were found to be composed of thin actin-like filaments (5-8 nm) and thick myosin-like filaments (10-15 nm X 300 nm). Actin filaments displayed changes in polarity (determined by S-1-myosin subfragment decoration), which may allow a sliding filament mechanism. At their pl

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Liu, Gongdai, R. Ghosh, A. Vaziri, A. Hossieni, D. Mousanezhad, and H. Nayeb-Hashemi. "Biomimetic composites inspired by venous leaf." Journal of Composite Materials 52, no. 3 (2017): 361–72. http://dx.doi.org/10.1177/0021998317707254.

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A typical plant leaf can be idealized as a composite having three principal fibers: the central mid-fiber corresponding to the mid-rib, straight parallel secondary fibers attached to the mid-fiber representing the secondary veins, and then another set of parallel fibers emanating from the secondary fibers mimicking the tertiary fibers embedded in a matrix material. This paper introduces a biomimetic composite design inspired by the morphology of venous leafs and investigates the effects of venation morphologies on the in-plane mechanical properties of the biomimetic composites using finite ele

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Wang, Mingyi, Kimberly R. McGraw, Robert E. Monticone, and Gianfranco Pintus. "Unraveling Elastic Fiber-Derived Signaling in Arterial Aging and Related Arterial Diseases." Biomolecules 15, no. 2 (2025): 153. https://doi.org/10.3390/biom15020153.

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Arterial stiffening is a significant risk factor for the development of cardiovascular diseases, including hypertension, atherosclerosis, and arteriopathy. The destruction of elastic fibers, accompanied by vascular inflammatory remodeling, is a key process in the progression of arterial stiffening and related pathologies. In young, healthy arteries, intact elastic fibers create a resilient microenvironment that maintains the quiescence of arterial cells. However, with advancing age, these elastic fibers undergo post-translational modifications, such as oxidation, glycosylation, and calcificati

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28

Fitts, R. H. "Cellular mechanisms of muscle fatigue." Physiological Reviews 74, no. 1 (1994): 49–94. http://dx.doi.org/10.1152/physrev.1994.74.1.49.

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Fatigue, defined as the failure to maintain the required or expected power output, is a complex problem, since multiple factors are clearly involved, with the relative importance of each dependent on the fiber type composition of the contracting muscles(s), and the intensity, type, and duration of the contractile activity. The primary sites of fatigue appear to be within the muscle cell itself and for the most part do not involve the central nervous system or the neuromuscular junction. The major hypotheses of fatigue center on disturbances in the surface membrane, E-C coupling, or metabolic e

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Toth, Michael J., Timothy W. Tourville, Thomas B. Voigt, et al. "Utility of Neuromuscular Electrical Stimulation to Preserve Quadriceps Muscle Fiber Size and Contractility After Anterior Cruciate Ligament Injuries and Reconstruction: A Randomized, Sham-Controlled, Blinded Trial." American Journal of Sports Medicine 48, no. 10 (2020): 2429–37. http://dx.doi.org/10.1177/0363546520933622.

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Background: Anterior cruciate ligament (ACL) injuries and reconstruction (ACLR) promote quadriceps muscle atrophy and weakness that can persist for years, suggesting the need for more effective rehabilitation programs. Whether neuromuscular electrical stimulation (NMES) can be used to prevent maladaptations in skeletal muscle size and function is unclear. Purpose: To examine whether early NMES use, started soon after an injury and maintained through 3 weeks after surgery, can preserve quadriceps muscle size and contractile function at the cellular (ie, fiber) level in the injured versus noninj

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Aratyn-Schaus, Yvonne, Patrick W. Oakes, and Margaret L. Gardel. "Dynamic and structural signatures of lamellar actomyosin force generation." Molecular Biology of the Cell 22, no. 8 (2011): 1330–39. http://dx.doi.org/10.1091/mbc.e10-11-0891.

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The regulation of cellular traction forces on the extracellular matrix is critical to cell adhesion, migration, proliferation, and differentiation. Diverse lamellar actin organizations ranging from contractile lamellar networks to stress fibers are observed in adherent cells. Although lamellar organization is thought to reflect the extent of cellular force generation, understanding of the physical behaviors of the lamellar actin cytoskeleton is lacking. To elucidate these properties, we visualized the actomyosin dynamics and organization in U2OS cells over a broad range of forces. At low force

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Liu, Yewei, Sarah J. Russell, and Martin F. Schneider. "Foxo1 nucleo-cytoplasmic distribution and unidirectional nuclear influx are the same in nuclei in a single skeletal muscle fiber but vary between fibers." American Journal of Physiology-Cell Physiology 314, no. 3 (2018): C334—C348. http://dx.doi.org/10.1152/ajpcell.00168.2017.

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Foxo transcription factors promote protein breakdown and atrophy of skeletal muscle fibers. Foxo transcriptional effectiveness is largely determined by phosphorylation-dependent nucleo-cytoplasmic shuttling. Imaging Foxo1-green fluorescent protein (GFP) over time in 124 nuclei in 68 multinucleated adult skeletal muscle fibers under control culture conditions reveals large variability between fibers in Foxo1-GFP nucleo-cytoplasmic concentration ratio (N/C) and in the apparent rate coefficient ( kI′) for Foxo1-GFP unidirectional nuclear influx (measured with efflux blocked by leptomycin B). Pair

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Welle, Stephen. "Cellular and Molecular Basis of Age-Related Sarcopenia." Canadian Journal of Applied Physiology 27, no. 1 (2002): 19–41. http://dx.doi.org/10.1139/h02-002.

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Sarcopenia, the decline in muscle bulk and performance associated with normal aging, is an important component of frailty in the elderly. The gradual loss of both motor nerves and muscle fibers during senescence appears to be the major problem. Atrophy (especially in fast-twitch fibers) and impaired function of the surviving cells also contribute to sarcopenia. Although skeletal muscle has the capacity to regenerate itself, this process is not activated by the gradual age-related loss of muscle fibers. The endocrine, autocrine, and paracrine environment in old muscle is less supportive of prot

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Shenkman, Boris S., Olga E. Zinovyeva, Svetlana P. Belova, et al. "Cellular and molecular signatures of alcohol-induced myopathy in women." American Journal of Physiology-Endocrinology and Metabolism 316, no. 5 (2019): E967—E976. http://dx.doi.org/10.1152/ajpendo.00513.2018.

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Alcoholic myopathy is characterized by the reduction in cross-sectional area (CSA) of muscle fibers and impaired anabolic signaling. The goal of the current study was to investigate the causes and compare the changes in CSA and fiber type composition with the modifications of anabolic and catabolic signaling pathways at the early stages of chronic alcohol consumption in women. Skeletal muscle samples from 5 female patients with alcohol abuse (AL; 43 ± 5 yr old; alcohol abuse duration 5,6 ± 0,6 yr) were compared with the muscle from the control group of 8 healthy women (C; 35 ± 4 yr old). The a

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Movahedian Attar, Fakhereh, Evgeniya Kirilina, Daniel Haenelt, et al. "Mapping Short Association Fibers in the Early Cortical Visual Processing Stream Using In Vivo Diffusion Tractography." Cerebral Cortex 30, no. 8 (2020): 4496–514. http://dx.doi.org/10.1093/cercor/bhaa049.

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Abstract Short association fibers (U-fibers) connect proximal cortical areas and constitute the majority of white matter connections in the human brain. U-fibers play an important role in brain development, function, and pathology but are underrepresented in current descriptions of the human brain connectome, primarily due to methodological challenges in diffusion magnetic resonance imaging (dMRI) of these fibers. High spatial resolution and dedicated fiber and tractography models are required to reliably map the U-fibers. Moreover, limited quantitative knowledge of their geometry and distribu

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Müller, Andreas, Sandra Müller, Veselin Nasufovic, Hans-Dieter Arndt, and Tilo Pompe. "Actin stress fiber dynamics in laterally confined cells." Integrative Biology 11, no. 5 (2019): 175–85. http://dx.doi.org/10.1093/intbio/zyz016.

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Abstract Multiple cellular processes are affected by spatial constraints from the extracellular matrix and neighboring cells. In vitro experiments using defined micro-patterning allow for in-depth analysis and a better understanding of how these constraints impact cellular behavior and functioning. Herein we focused on the analysis of actin cytoskeleton dynamics as a major determinant of mechanotransduction mechanisms in cells. We seeded primary human umbilical vein endothelial cells onto stripe-like cell-adhesive micro-patterns with varying widths and then monitored and quantified the dynamic

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Xu, Peilong, Qinghui Yuan, Wendong Ji, Ruitao Yu, Fengyun Wang, and Ningbo Huo. "Study on the annealing phase transformation mechanism and electrochemical properties of carbon submicron fibers loaded with cobalt." Materials Express 12, no. 12 (2022): 1493–501. http://dx.doi.org/10.1166/mex.2022.2302.

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Heterogeneous carbon submicron fibers loaded with Co3O4/Co and elemental cobalt were prepared by electrospinning and annealing processes. The phase transformation mechanism of Co3O4 and elemental cobalt in heterogeneous submicron fibers at different annealing temperatures was studied. The electrochemical properties of carbon submicron fiber self-supported electrode materials loaded with Co3O4/Co and elemental cobalt were investigated. The experimental results show that the submicron fibers loaded with Co3O4/Co have better electrochemical performance because Co3O4 has large capacitance, the imp

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Bagchi, Amrit, Bapi Sarker, Jialiang Zhang, Marcus Foston, and Amit Pathak. "Fast yet force-effective mode of supracellular collective cell migration due to extracellular force transmission." PLOS Computational Biology 21, no. 1 (2025): e1012664. https://doi.org/10.1371/journal.pcbi.1012664.

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Cell collectives, like other motile entities, generate and use forces to move forward. Here, we ask whether environmental configurations alter this proportional force-speed relationship, since aligned extracellular matrix fibers are known to cause directed migration. We show that aligned fibers serve as active conduits for spatial propagation of cellular mechanotransduction through matrix exoskeleton, leading to efficient directed collective cell migration. Epithelial (MCF10A) cell clusters adhered to soft substrates with aligned collagen fibers (AF) migrate faster with much lesser traction fo

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Callahan, Damien M., Nicholas G. Bedrin, Meenakumari Subramanian, et al. "Age-related structural alterations in human skeletal muscle fibers and mitochondria are sex specific: relationship to single-fiber function." Journal of Applied Physiology 116, no. 12 (2014): 1582–92. http://dx.doi.org/10.1152/japplphysiol.01362.2013.

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Age-related loss of skeletal muscle mass and function is implicated in the development of disease and physical disability. However, little is known about how age affects skeletal muscle structure at the cellular and ultrastructural levels or how such alterations impact function. Thus we examined skeletal muscle structure at the tissue, cellular, and myofibrillar levels in young (21–35 yr) and older (65–75 yr) male and female volunteers, matched for habitual physical activity level. Older adults had smaller whole muscle tissue cross-sectional areas (CSAs) and mass. At the cellular level, older

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Castorena, Carlos M., Edward B. Arias, Naveen Sharma, Jonathan S. Bogan, and Gregory D. Cartee. "Fiber type effects on contraction-stimulated glucose uptake and GLUT4 abundance in single fibers from rat skeletal muscle." American Journal of Physiology-Endocrinology and Metabolism 308, no. 3 (2015): E223—E230. http://dx.doi.org/10.1152/ajpendo.00466.2014.

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To fully understand skeletal muscle at the cellular level, it is essential to evaluate single muscle fibers. Accordingly, the major goals of this study were to determine if there are fiber type-related differences in single fibers from rat skeletal muscle for: 1) contraction-stimulated glucose uptake and/or 2) the abundance of GLUT4 and other metabolically relevant proteins. Paired epitrochlearis muscles isolated from Wistar rats were either electrically stimulated to contract (E-Stim) or remained resting (No E-Stim). Single fibers isolated from muscles incubated with 2-deoxy-d-[3H]glucose (2-

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Catur, A. D., P. D. Setyawan, Y. A. Padang, I. M. Nuarsa, and A. A. A. Triadi. "Kuat lentur dan berat jenis beton ringan selular diperkuat serat kain pakaian bekas." Dinamika Teknik Mesin 12, no. 1 (2022): 8. http://dx.doi.org/10.29303/dtm.v12i1.497.

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Cellular lightweight concrete has an advantage in its specific gravity, with the presence of foam making it lighter. However, the presence of foam reduces its strength. Utilization of used clothing fibers is carried out to increase the flexural strength of cellular lightweight concrete and is useful for reducing environmental loads. Used clothing fibers are added to the cellular lightweight concrete in the amount of 0%; 0.3%; 0.6%; 0.9% and 1.2% of weight. Cellular lightweight concrete is also reinforced with steel wire mesh with a wire diameter of 0.5 mm with a side length of ¼ inch x ¼ inch.

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Bjørnsen, Thomas, Mathias Wernbom, Gøran Paulsen, et al. "High-frequency blood flow-restricted resistance exercise results in acute and prolonged cellular stress more pronounced in type I than in type II fibers." Journal of Applied Physiology 131, no. 2 (2021): 643–60. http://dx.doi.org/10.1152/japplphysiol.00115.2020.

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BFRRE has been reported to preferentially stress type I muscle fibers, as evidenced by HSP responses. We extend these findings by showing that the HSP responses occur in both fiber types but more so in type I fibers and that they can still be induced after a short-term training period. Furthermore, the reductions in glycogen content of type I fibers after strenuous frequent BFRRE in unaccustomed subjects can be prolonged (≥5 days), probably due to microdamage.

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Faglie, Addison, Rachel Emerine, and Shih-Feng Chou. "Effects of Poloxamers as Excipients on the Physicomechanical Properties, Cellular Biocompatibility, and In Vitro Drug Release of Electrospun Polycaprolactone (PCL) Fibers." Polymers 15, no. 14 (2023): 2997. http://dx.doi.org/10.3390/polym15142997.

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Electrospun microfibers are emerging as one of the advanced wound dressing materials for acute and/or chronic wounds, especially with their ability to carry drugs and excipients at a high loading while being able to deliver them in a controlled manner. Various attempts were made to include excipients in electrospun microfibers as wound dressing materials, and one of them is poloxamer, an amphiphilic polymer that exhibits wound debridement characteristics. In this study, we formulated two types of poloxamers (i.e., P188 and P338) at 30% (w/w) loading into electrospun polycaprolactone (PCL) fibe

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Murgia, Marta, Leonardo Nogara, Martina Baraldo, Carlo Reggiani, Matthias Mann, and Stefano Schiaffino. "Protein profile of fiber types in human skeletal muscle: a single-fiber proteomics study." Skeletal Muscle 11, no. 1 (2021): 24. https://doi.org/10.1186/s13395-021-00279-0.

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<strong>Background: </strong>Human skeletal muscle is composed of three major fiber types, referred to as type 1, 2A, and 2X fibers. This heterogeneous cellular composition complicates the interpretation of studies based on whole skeletal muscle lysate. A single-fiber proteomics approach is required to obtain a fiber-type resolved quantitative information on skeletal muscle pathophysiology.<strong>Methods: </strong>Single fibers were dissected from vastus lateralis muscle biopsies of young adult males and processed for mass spectrometry-based single-fiber proteomics. We provide and analyze a r

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White, G. E., and K. Fujiwara. "Expression and intracellular distribution of stress fibers in aortic endothelium." Journal of Cell Biology 103, no. 1 (1986): 63–70. http://dx.doi.org/10.1083/jcb.103.1.63.

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Immunofluorescence microscopy was used to determine the number of endothelial cells with stress fibers for three age groups, and for three distinct anatomical locations within the descending thoracic aorta of both normotensive and spontaneously hypertensive rats. For each age group examined, hypertensive rats consistently demonstrated greater stress fiber expression than did normotensive rats. Neither age nor blood pressure was the predominant influence on stress fiber expression in aortic endothelium. In the normotensive rats, stress fiber expression remained unchanged for all age groups exam

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Zheng, Qian, Elaine C. Davis, James A. Richardson, et al. "Molecular Analysis of Fibulin-5 Function during De Novo Synthesis of Elastic Fibers." Molecular and Cellular Biology 27, no. 3 (2007): 1083–95. http://dx.doi.org/10.1128/mcb.01330-06.

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ABSTRACT Elastic fibers contribute to the structural support of tissues and to the regulation of cellular behavior. Mice deficient for the fibulin-5 gene (fbln5 − / −) were used to further elucidate the molecular mechanism of elastic fiber assembly. Major elastic fiber components were present in the skin of fbln5 − / − mice despite a dramatic reduction of mature elastic fibers. We found that fibulin-5 preferentially bound the monomeric form of elastin through N-terminal and C-terminal elastin-binding regions and to a preexisting matrix scaffold through calcium-binding epidermal growth factor (

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Domura, Ryota, Rie Sasaki, Masami Okamoto, et al. "Comprehensive study on cellular morphologies, proliferation, motility, and epithelial–mesenchymal transition of breast cancer cells incubated on electrospun polymeric fiber substrates." Journal of Materials Chemistry B 5, no. 14 (2017): 2588–600. http://dx.doi.org/10.1039/c7tb00207f.

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Stallcup, William B., and Kimberlee Dahlin-Huppe. "Chondroitin sulfate and cytoplasmic domain-dependent membrane targeting of the NG2 proteoglycan promotes retraction fiber formation and cell polarization." Journal of Cell Science 114, no. 12 (2001): 2315–25. http://dx.doi.org/10.1242/jcs.114.12.2315.

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Targeting of the NG2 proteoglycan to cellular retraction fibers was studied by expressing mutant NG2 molecules lacking specific structural elements of the proteoglycan. Both the cytoplasmic domain and the chondroitin sulfate chain of NG2 appear to have roles in sorting NG2 to subcellular microdomains destined to become retraction fibers. Neither of these structural features alone is sufficient to allow optimal targeting of NG2 to retraction fibers, but together they promote efficient localization of the proteoglycan to these sites. This pattern of NG2 sorting seems to be necessary for optimal

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Chandra, Murali, Matthew L. Tschirgi, and Jil C. Tardiff. "Increase in tension-dependent ATP consumption induced by cardiac troponin T mutation." American Journal of Physiology-Heart and Circulatory Physiology 289, no. 5 (2005): H2112—H2119. http://dx.doi.org/10.1152/ajpheart.00571.2005.

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How different mutations in cardiac troponin T (cTnT) lead to distinct secondary downstream cellular remodeling in familial hypertrophic cardiomyopathy (FHC) remains elusive. To explore the molecular basis for the distinct impact of different mutations in cTnT on cardiac myocytes, we studied mechanical activity of detergent-skinned muscle fiber bundles from different lines of transgenic (TG) mouse hearts that express wild-type cTnT (WTTG), R92W cTnT, R92L cTnT, and Delta-160 cTnT (deletion of amino acid 160). The amount of mutant cTnT is ∼50% of the total myocellular cTnT in both R92W and R92L

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Schuh, Rosemary A., Kathryn C. Jackson, Ramzi J. Khairallah, Christopher W. Ward, and Espen E. Spangenburg. "Measuring mitochondrial respiration in intact single muscle fibers." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 302, no. 6 (2012): R712—R719. http://dx.doi.org/10.1152/ajpregu.00229.2011.

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Measurement of mitochondrial function in skeletal muscle is a vital tool for understanding regulation of cellular bioenergetics. Currently, a number of different experimental approaches are employed to quantify mitochondrial function, with each involving either mechanically or chemically induced disruption of cellular membranes. Here, we describe a novel approach that allows for the quantification of substrate-induced mitochondria-driven oxygen consumption in intact single skeletal muscle fibers isolated from adult mice. Specifically, we isolated intact muscle fibers from the flexor digitorum

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Shinohara, Harumichi, Xiaoliang Liu, Riho Nakajima, et al. "Pyramid-Shape Crossings and Intercrossing Fibers Are Key Elements for Construction of the Neural Network in the Superficial White Matter of the Human Cerebrum." Cerebral Cortex 30, no. 10 (2020): 5218–28. http://dx.doi.org/10.1093/cercor/bhaa080.

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Abstract Structural analysis of the superficial white matter is prerequisite for the understanding of highly integrated functions of the human cerebral cortex. However, the principal components, U-fibers, have been regarded as simple wires to connect adjacent gyri (inter-gyral U-fibers) but have never been thought as indispensable elements of anatomical structures to construct the cortical network. Here, we reported such novel structures made of U-fibers. Seven human cerebral hemispheres were treated with Klingler’s method and subjected to fiber dissection (FD). Additionally, tractography usin

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