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Journal articles on the topic 'Cellulase cocktail'

Author: Grafiati

Published: 4 June 2021

Last updated: 14 February 2022

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1

Contreras, Francisca, Subrata Pramanik, Aleksandra M. Rozhkova, et al. "Engineering Robust Cellulases for Tailored Lignocellulosic Degradation Cocktails." International Journal of Molecular Sciences 21, no. 5 (2020): 1589. http://dx.doi.org/10.3390/ijms21051589.

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Lignocellulosic biomass is a most promising feedstock in the production of second-generation biofuels. Efficient degradation of lignocellulosic biomass requires a synergistic action of several cellulases and hemicellulases. Cellulases depolymerize cellulose, the main polymer of the lignocellulosic biomass, to its building blocks. The production of cellulase cocktails has been widely explored, however, there are still some main challenges that enzymes need to overcome in order to develop a sustainable production of bioethanol. The main challenges include low activity, product inhibition, and th

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2

Bernardi, Aline Vianna, Luis Eduardo Gerolamo, Paula Fagundes de Gouvêa, et al. "LPMO AfAA9_B and Cellobiohydrolase AfCel6A from A. fumigatus Boost Enzymatic Saccharification Activity of Cellulase Cocktail." International Journal of Molecular Sciences 22, no. 1 (2020): 276. http://dx.doi.org/10.3390/ijms22010276.

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Cellulose is the most abundant polysaccharide in lignocellulosic biomass, where it is interlinked with lignin and hemicellulose. Bioethanol can be produced from biomass. Since breaking down biomass is difficult, cellulose-active enzymes secreted by filamentous fungi play an important role in degrading recalcitrant lignocellulosic biomass. We characterized a cellobiohydrolase (AfCel6A) and lytic polysaccharide monooxygenase LPMO (AfAA9_B) from Aspergillus fumigatus after they were expressed in Pichia pastoris and purified. The biochemical parameters suggested that the enzymes were stable; the o

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3

Thoresen, Mariska, Samkelo Malgas, Mpho Mafa, and Brett Pletschke. "Revisiting the Phenomenon of Cellulase Action: Not All Endo- and Exo-Cellulase Interactions Are Synergistic." Catalysts 11, no. 2 (2021): 170. http://dx.doi.org/10.3390/catal11020170.

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The conventional endo–exo synergism model has extensively been supported in literature, which is based on the perception that endoglucanases (EGs) expose or create accessible sites on the cellulose chain to facilitate the action of processive cellobiohydrolases (CBHs). However, there is a lack of information on why some bacterial and fungal CBHs and EGs do not exhibit synergism. Therefore, the present study evaluated and compared the synergistic relationships between cellulases from different microbial sources and provided insights into how different GH families govern synergism. The results s

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4

Malgas, Samkelo, Shaunita H. Rose, Willem H. van Zyl, and Brett I. Pletschke. "Enzymatic Hydrolysis of Softwood Derived Paper Sludge by an In Vitro Recombinant Cellulase Cocktail for the Production of Fermentable Sugars." Catalysts 10, no. 7 (2020): 775. http://dx.doi.org/10.3390/catal10070775.

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Paper sludge is an attractive biomass feedstock for bioconversion to ethanol due to its low cost and the lack of pretreatment required for its bioprocessing. This study assessed the use of a recombinant cellulase cocktail (mono-components: S. cerevisiae-derived PcBGL1B (BGL), TeCel7A (CBHI), ClCel6A (CBHII) and TrCel5A (EGII) mono-component cellulase enzymes) for the efficient saccharification of softwood-derived paper sludge to produce fermentable sugars. The paper sludge mainly contained 74.3% moisture and 89.7% (per dry mass (DM)) glucan with a crystallinity index of 91.5%. The optimal prot

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5

Srivastava, Neha, Abdallah M. Elgorban, P. K. Mishra, et al. "Enhance production of fungal cellulase cocktail using cellulosic waste." Environmental Technology & Innovation 19 (August 2020): 100949. http://dx.doi.org/10.1016/j.eti.2020.100949.

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6

Chang, Jui-Jen, Yu-Ju Lin, Chyi-How Lay, et al. "Constructing a cellulosic yeast host with an efficient cellulase cocktail." Biotechnology and Bioengineering 115, no. 3 (2017): 751–61. http://dx.doi.org/10.1002/bit.26507.

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7

Soleimani, Saeed, and Seyed-Omid Ranaei-Siadat. "Preparation and optimization of cellulase cocktail to improve the bioethanol process." Biofuels 8, no. 2 (2016): 291–96. http://dx.doi.org/10.1080/17597269.2016.1224293.

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8

DAS, ARPAN, TANMAY PAUL, SUMAN KUMAR HALDER, et al. "Study on Regulation of Growth and Biosynthesis of Cellulolytic Enzymes from Newly Isolated Aspergillus fumigatus ABK9." Polish Journal of Microbiology 62, no. 1 (2013): 31–43. http://dx.doi.org/10.33073/pjm-2013-004.

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This study was aimed to evaluate the pattern of cellulase biosynthesis from Aspergillusfumigatus ABK9 under submerged fermentation. Production was increased concomitantly with fungal growth up to 72 h and reached maximum (Xmax -6.72 g/l) with specific growth rate (mu max) of 0.126/h. Highest specific rate of enzyme production (q ) was found at initial medium pH of 5.0 and incubation temperature of 30 degrees C. At the same time, in the presence of 2-deoxy-D-glucose concentration of 0.5 mg/ml, the production of cellulolytic enzymes, viz, carboxymethyl cellulase activity (CMCase), filter paper d

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9

Wang, Dan, Jie Sun, Hui-Lei Yu, Chun-Xiu Li, Jie Bao, and Jian-He Xu. "Maximum Saccharification of Cellulose Complex by an Enzyme Cocktail Supplemented with Cellulase from Newly Isolated Aspergillus fumigatus ECU0811." Applied Biochemistry and Biotechnology 166, no. 1 (2011): 176–86. http://dx.doi.org/10.1007/s12010-011-9414-9.

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10

Park, Joshua I., Eric J. Steen, Helcio Burd, et al. "A Thermophilic Ionic Liquid-Tolerant Cellulase Cocktail for the Production of Cellulosic Biofuels." PLoS ONE 7, no. 5 (2012): e37010. http://dx.doi.org/10.1371/journal.pone.0037010.

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11

Masran, Ruqayyah, Ezyana Kamal Bahrin, Mohamad Faizal Ibrahim, Lai-Yee Phang, and Suraini Abd-Aziz. "Simultaneous pretreatment and saccharification of oil palm empty fruit bunch using laccase-cellulase cocktail." Biocatalysis and Agricultural Biotechnology 29 (October 2020): 101824. http://dx.doi.org/10.1016/j.bcab.2020.101824.

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12

Cai, Linian, Shengnan Xu, Tao Lu, Dongqiang Lin, and Shanjing Yao. "Salt-tolerant mechanism of marine Aspergillus niger cellulase cocktail and improvement of its activity." Chinese Journal of Chemical Engineering 28, no. 4 (2020): 1120–28. http://dx.doi.org/10.1016/j.cjche.2019.11.012.

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13

Takano, Maki, and Kazuhiro Hoshino. "PRODUCTION OF BIOFUEL FROM WASTE LIGNOCELLULOSIC BIOMASS MATERIALS BASED ON ENERGY SAVING VIEWPOINT." International Journal of Modern Physics: Conference Series 06 (January 2012): 715–20. http://dx.doi.org/10.1142/s2010194512004035.

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To develop biofuel production from waste lignocellulosic biomass materials the rice straw was selected one of renewable material and the degradation condition about pretreatment and enzymatic hydrolysis to obtain effectively fermentable sugars was investigated. Rice straw was pretreated by five kinds of methods and then the components ratio of rice straw was examined. First, the steam explosion was selected based on the degradability and the requirement energy. In addition, the best suitable combination of two cellulases to effective and economical hydrolyze was determined from the degradabili

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14

Yamada, Ryosuke, Toshihide Yoshie, Shoji Sakai, et al. "Effective saccharification of kraft pulp by using a cellulase cocktail prepared from genetically engineeredAspergillus oryzae." Bioscience, Biotechnology, and Biochemistry 79, no. 6 (2015): 1034–37. http://dx.doi.org/10.1080/09168451.2015.1006568.

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15

Chang, Jui-Jen, Feng-Ju Ho, Cheng-Yu Ho, et al. "Assembling a cellulase cocktail and a cellodextrin transporter into a yeast host for CBP ethanol production." Biotechnology for Biofuels 6, no. 1 (2013): 19. http://dx.doi.org/10.1186/1754-6834-6-19.

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16

Maleki, Morteza, Mehdi Foroozandeh Shahraki, Kaveh Kavousi, Shohreh Ariaeenejad, and Ghasem Hosseini Salekdeh. "A novel thermostable cellulase cocktail enhances lignocellulosic bioconversion and biorefining in a broad range of pH." International Journal of Biological Macromolecules 154 (July 2020): 349–60. http://dx.doi.org/10.1016/j.ijbiomac.2020.03.100.

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17

Kazeem, Muinat Olanike, Umi Kalsom Md Shah, Azhari Samsu Baharuddin, and Nor’ Aini AbdulRahman. "Prospecting Agro-waste Cocktail: Supplementation for Cellulase Production by a Newly Isolated Thermophilic B. licheniformis 2D55." Applied Biochemistry and Biotechnology 182, no. 4 (2017): 1318–40. http://dx.doi.org/10.1007/s12010-017-2401-z.

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18

Junior, Nei Pereira, Anelize de Oliveira Moraes, Luiz Felipe Modesto, and Ninoska Isabel Bojorge Ramirez. "Reuse of Residual Biomass of Cellulose Industry for Second Generation Bioethanol Production." JOURNAL OF ADVANCES IN BIOTECHNOLOGY 6, no. 1 (2016): 768–72. http://dx.doi.org/10.24297/jbt.v6i1.4805.

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This study aimed at evaluating the potential of pulp mill residue (PMR) as a feedstock for ethanol production. The simultaneous saccharification and fermentation (SSF) process was operated using 8 gL -1 of a commercial strain of Saccharomyces cerevisiae JP1 under optimal proportions of cellulase cocktail (24.8 FPU/g cellulose of CellicÂ® CTec2) and cellulosic residue (200 gL -1 ). After 48 hours of pre-hydrolysis at 50ÂºC and 200 rpm, the fermentation was carried out at 37 ÂºC, generating 48.5 gL -1 of ethanol in 10 hours and reaching a conversion efficiency of 53.3% from cellulose to ethanol

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19

Rajan, Kalavathy, та Danielle Julie Carrier. "Characterization of Rice Straw Prehydrolyzates and Their Effect on the Hydrolysis of Model Substrates Using a Commercial endo-Cellulase, β-Glucosidase and Cellulase Cocktail". ACS Sustainable Chemistry & Engineering 2, № 9 (2014): 2124–30. http://dx.doi.org/10.1021/sc5002947.

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20

Phan, Minh Thi Tuyet, Viet Quoc Nguyen, Hy Gia Le, Thoa Kim Nguyen, and Man Dinh Tran. "Molecular cloning gene and nucleotide sequence of the gene encoding an endo-1,4-beta-glucanase from Bacillus sp VLSH08 strain applying to biomass hydrolysis." Journal of Vietnamese Environment 3, no. 2 (2012): 80–86. http://dx.doi.org/10.13141/jve.vol3.no2.pp80-86.

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Bacillus sp VLSH08 screened from sea wetland in Nam Dinh province produces an extracellular endo-1,4-beta-glucanase. According to the results of the classified Kit API 50/CHB as well as sequence of 1500 bp fragment coding for 16S rRNA gene of the Bacillus sp VLSH 08 strain showed that the taxonomical characteristics between the strain VLSH 08 and Bacillus amyloliquefaciene JN999857 are similar of 98%. Culture supernatant of this strain showed optimal cellulase activity at pH 5.8 and 60°C and that was enhanced 2.03 times in the presence of 5 mM Co2+. Moreover, the gene encoding endo-1,4-beta-gl

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21

Zhang, Yu, Jinshui Yang, Lijin Luo, et al. "Low-Cost Cellulase-Hemicellulase Mixture Secreted by Trichoderma harzianum EM0925 with Complete Saccharification Efficacy of Lignocellulose." International Journal of Molecular Sciences 21, no. 2 (2020): 371. http://dx.doi.org/10.3390/ijms21020371.

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Fermentable sugars are important intermediate products in the conversion of lignocellulosic biomass to biofuels and other value-added bio-products. The main bottlenecks limiting the production of fermentable sugars from lignocellulosic biomass are the high cost and the low saccharification efficiency of degradation enzymes. Herein, we report the secretome of Trichoderma harzianum EM0925 under induction of lignocellulose. Numerously and quantitatively balanced cellulases and hemicellulases, especially high levels of glycosidases, could be secreted by T. harzianum EM0925. Compared with the comme

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22

Del Pozo, Mercedes V., Lucía Fernández-Arrojo, Jorge Gil-Martínez та ін. "Microbial β-glucosidases from cow rumen metagenome enhance the saccharification of lignocellulose in combination with commercial cellulase cocktail". Biotechnology for Biofuels 5, № 1 (2012): 73. http://dx.doi.org/10.1186/1754-6834-5-73.

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23

Sawant, Shailesh S., Bipinchandra K. Salunke, and Beom Soo Kim. "Degradation of corn stover by fungal cellulase cocktail for production of polyhydroxyalkanoates by moderate halophile Paracoccus sp. LL1." Bioresource Technology 194 (October 2015): 247–55. http://dx.doi.org/10.1016/j.biortech.2015.07.019.

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24

Du, Jian, Jingrui Liang, Xiuhua Gao, Guodong Liu, and Yinbo Qu. "Optimization of an artificial cellulase cocktail for high-solids enzymatic hydrolysis of cellulosic materials with different pretreatment methods." Bioresource Technology 295 (January 2020): 122272. http://dx.doi.org/10.1016/j.biortech.2019.122272.

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25

Long, Liangkun, Lu Sun, Dafan Ding, Kaixiang Chen, Qunying Lin, and Shaojun Ding. "Two C1-oxidizing lytic polysaccharide monooxygenases from Ceriporiopsis subvermispora enhance the saccharification of wheat straw by a commercial cellulase cocktail." Process Biochemistry 110 (November 2021): 243–50. http://dx.doi.org/10.1016/j.procbio.2021.08.013.

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26

Thite, Vihang S., and Anuradha S. Nerurkar. "Crude Xylanases and Pectinases from Bacillus spp. Along with Commercial Cellulase Formulate an Efficient Tailor-Made Cocktail for Sugarcane Bagasse Saccharification." BioEnergy Research 13, no. 1 (2019): 286–300. http://dx.doi.org/10.1007/s12155-019-10050-5.

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27

Martins, Igor Magno Nicurgo Borges Rosa, Luanna Stefanny Vieira Oliveira Gomes, Daniel Pasquini, and Milla Alves Baffi. "Production and characterization of cellulases and hemicellulases from a consortium between Pleurotus ostreatus and Aspergillus niger cultured in agro-industrial wastes." Research, Society and Development 10, no. 10 (2021): e396101019020. http://dx.doi.org/10.33448/rsd-v10i10.19020.

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The enzyme biosynthesis using agricultural wastes by solid state fermentation (SSF) and the study of their physicochemical properties are meaningful approaches to improve the biomass hydrolysis. Among them, β-glucosidases and β-xylosidases are key enzymes at the lignocellulose depolymerization, which act in the cleavage of oligosaccharides in monosaccharides. In this study, the production of hemicellulases and cellulases by Pleurotus ostreatus and Aspergillus niger monocultures or in consortium was investigated, using raw sugarcane bagasse (SB) and wheat bran (WB) as substrates. The highest en

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28

Vats, Anumeha, Anuj Sangam Kurade, and Srikanth Mutnuri. "Recovery of Lignocellulolytic Enzymes and Valorization of Spent Mushroom Substrate." Environment and Natural Resources Journal 20, no. 1 (2021): 1–9. http://dx.doi.org/10.32526/ennrj/20/202100099.

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Spent Mushroom Substrate (SMS) comprises sugarcane bagasse, coconut coir, chicken manure, and paddy straw; inoculated with and farmed for Agaricus bisporus. At present, the waste generation at a mushroom cultivation plant in Goa is 40 tons/day (15,000 tons annually). Valorization of this waste has been explored in terms of extracting lignocellulolytic enzymes and briquette production. SMS was screened for the presence of lignocellulolytic enzymes and then was used to make briquettes. The enzymes found in SMS were cellulase and laccase, which were further concentrated via tangential flow filtra

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Popescu, Mariana, Florin Oancea, Elena Radu, Mălina Deșliu-Avram, and Călina Petruța Cornea. "Bioconversion of Oilseeds into Bioproducts for Cultivated Plants Nutrition and Protection." “Agriculture for Life, Life for Agriculture” Conference Proceedings 1, no. 1 (2018): 572–78. http://dx.doi.org/10.2478/alife-2018-0090.

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AbstractNatural by-products from vegetable oil industries and spent edible oils from domestic or public food spaces should be recycled to obtain new added value products. Present paper proposed a technical solution for complete valorisation of inedible oilseeds or spent edible oils into bioproducts for nutrition and protection of plants cultivated in conservative organic agrosystems. Pressed cakes resulted from mechanical cold extraction of mustard oil contain residual oil and bioactive compounds which were released using an enzymatic cocktail 1:1 cellulase with proteases coupled with azeotrop

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Jain, Lavika, Akhilesh Kumar Kurmi, Avnish Kumar, Anand Narani, Thallada Bhaskar, and Deepti Agrawal. "Exploring the flexibility of cellulase cocktail obtained from mutant UV-8 of Talaromyces verruculosus IIPC 324 in depolymerising multiple agro-industrial lignocellulosic feedstocks." International Journal of Biological Macromolecules 154 (July 2020): 538–44. http://dx.doi.org/10.1016/j.ijbiomac.2020.03.133.

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31

Victoria, Juliet, Annamma Odaneth, and Arvind Lali. "Importance of cellulase cocktails favoring hydrolysis of cellulose." Preparative Biochemistry & Biotechnology 47, no. 6 (2017): 547–53. http://dx.doi.org/10.1080/10826068.2016.1275006.

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32

Nwamba, Marknoah Chinenye, Guojie Song, Fubao Sun, et al. "Efficiency enhancement of a new cellulase cocktail at low enzyme loading for high solid digestion of alkali catalyzed atmospheric glycerol organosolvent pre-treated sugarcane bagasse." Bioresource Technology 338 (October 2021): 125505. http://dx.doi.org/10.1016/j.biortech.2021.125505.

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Marzo, C., A. B. Díaz, I. Caro, and A. Blandino. "Valorization of agro-industrial wastes to produce hydrolytic enzymes by fungal solid-state fermentation." Waste Management & Research: The Journal for a Sustainable Circular Economy 37, no. 2 (2018): 149–56. http://dx.doi.org/10.1177/0734242x18798699.

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Nowadays, significant amounts of agro-industrial wastes are discarded by industries; however, they represent interesting raw materials for the production of high-added value products. In this regard, orange peels (ORA) and exhausted sugar beet cossettes (ESBC) have turned out to be promising raw materials for hydrolytic enzymes production by solid state fermentation (SSF) and also a source of sugars which could be fermented to different high-added value products. The maximum activities of xylanase and exo-polygalacturonase (exo-PG) measured in the enzymatic extracts obtained after the SSF of O

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Purwadari, Tresnawati, Pius P. Ketaren, Arnold P. Sinurat, and Irawan Sutikno. "IDENTIFICATION AND EVALUATION OF FIBER HYDROLYTIC ENZYMES IN THE EXTRACT OF TERMITES (Glyptotermes montanus) FOR POULTRY FEED APPLICATION." Indonesian Journal of Agricultural Science 4, no. 2 (2016): 40. http://dx.doi.org/10.21082/ijas.v4n2.2003.40-47.

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Foot rot disease of black pepper caused by Phytophthora capsici had been reported in Batangas and Laguna, Philippines. The plant was recovered following the application of crop residue (organic substrate) and intercropping with other crops. This study was aimed to isolate, identify, and determine the soil mycoflora from the rhizosphere of black pepper grown on various cropping patterns in Batangas and Laguna. Antagonistic activity of mycoflora isolates was tested against P. capsici using dual culture technique. The result showed that 149 colonies of soil mycoflora isolated were belonging to 14

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Purwadari, Tresnawati, Pius P. Ketaren, Arnold P. Sinurat, and Irawan Sutikno. "IDENTIFICATION AND EVALUATION OF FIBER HYDROLYTIC ENZYMES IN THE EXTRACT OF TERMITES (Glyptotermes montanus) FOR POULTRY FEED APPLICATION." Indonesian Journal of Agricultural Science 4, no. 2 (2016): 40. http://dx.doi.org/10.21082/ijas.v4n2.2003.p40-47.

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Foot rot disease of black pepper caused by Phytophthora capsici had been reported in Batangas and Laguna, Philippines. The plant was recovered following the application of crop residue (organic substrate) and intercropping with other crops. This study was aimed to isolate, identify, and determine the soil mycoflora from the rhizosphere of black pepper grown on various cropping patterns in Batangas and Laguna. Antagonistic activity of mycoflora isolates was tested against P. capsici using dual culture technique. The result showed that 149 colonies of soil mycoflora isolated were belonging to 14

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36

Thite, V. S., and A. S. Nerurkar. "Physicochemical characterization of pectinase activity from Bacillus spp. and their accessory role in synergism with crude xylanase and commercial cellulase in enzyme cocktail mediated saccharification of agrowaste biomass." Journal of Applied Microbiology 124, no. 5 (2018): 1147–63. http://dx.doi.org/10.1111/jam.13718.

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Chen, Yuxia, Dan Shen, Lilan Zhang, et al. "Supplementation of Non-Starch Polysaccharide Enzymes Cocktail in a Corn-Miscellaneous Meal Diet Improves Nutrient Digestibility and Reduces Carbon Dioxide Emissions in Finishing Pigs." Animals 10, no. 2 (2020): 232. http://dx.doi.org/10.3390/ani10020232.

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This study was carried out to evaluate the effect of the addition of the non-starch polysaccharide enzymes cocktail (NSPEC) on growth performance, nutrient digestibility and gas emissions in a corn-miscellaneous meal-based diet for finishing pigs. The NSPEC is a combination of cellulase, xylanase, β-glucanase, β-mannanase, α-galactosidase and pectinase optimized by assessing the in vitro dry matter digestibility (IVDMD) of corn-miscellaneous meal diet using an in vitro method of simulating digestion in the stomach and intestine of growing pigs. Growth performance and apparent total tract diges

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D. Costa, Vera, Ana Costa, Maria Amaral, and Rogério S. Simões. "A Comprehensive Approach of Eucalyptus globulus Acid Sulfite Pretreatment for Enzymatic Hydrolysis." Applied Sciences 10, no. 11 (2020): 3764. http://dx.doi.org/10.3390/app10113764.

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The effect of different acid sulfite pretreatment conditions on released components in the hydrolysates and the pretreated solid residues’ response to enzymatic hydrolysis for Eucalyptus globulus chips was investigated. Sodium bisulfite (0–15%), and sulfuric acid (0–5%) were used to pretreat chips at 170 °C and 190 °C, for as long as 30 min. The hydrolysates were analyzed through high-performance liquid chromatography (HPLC) and spectrophotometry. Overall porosity and pores larger than 2.65 nm (size of a typical cellulase) on the solid residues were estimated using glucose and two dextrans wit

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Favaro, Camila, Ilton Baraldi, Fernanda Casciatori та Cristiane Farinas. "β-Mannanase Production Using Coffee Industry Waste for Application in Soluble Coffee Processing". Biomolecules 10, № 2 (2020): 227. http://dx.doi.org/10.3390/biom10020227.

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Soluble coffee offers the combined benefits of high added value and practicality for its consumers. The hydrolysis of coffee polysaccharides by the biochemical route, using enzymes, is an eco-friendly and sustainable way to improve the quality of this product, while contributing to the implementation of industrial processes that have lower energy requirements and can reduce environmental impacts. This work describes the production of hydrolytic enzymes by solid-state fermentation (SSF), cultivating filamentous fungi on waste from the coffee industry, followed by their application in the hydrol

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Antov, Mirjana, and Aleksandar Fistes. "The effect of beta-glucosidase supplementation on enzymatic hydrolysis of cellulose in hydrothermally pretreated sugar beet shreds." Acta Periodica Technologica, no. 49 (2018): 1–9. http://dx.doi.org/10.2298/apt1849001a.

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Sugar beet shreds were pretreated by hydrothermal procedure to investigate the effect of beta-glucosidase supplementation at different substrate loading on the rate of cellulose hydrolysis. Cellulose in the hydrothermally pretreated substrate was more efficiently hydrolyzed by enzymes than in untreated material, resulting in more than two times higher release of reducing sugars. In the investigated range of solids load, supplementation of fungal cellulases cocktail by beta-glucosidase increased production of reducing sugars from substrates, while 0.25 U/g was sufficient to achieve the highest

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Liang, Youyun, Tong Si, Ee Lui Ang, and Huimin Zhao. "Engineered Pentafunctional Minicellulosome for Simultaneous Saccharification and Ethanol Fermentation in Saccharomyces cerevisiae." Applied and Environmental Microbiology 80, no. 21 (2014): 6677–84. http://dx.doi.org/10.1128/aem.02070-14.

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ABSTRACTSeveral yeast strains have been engineered to express different cellulases to achieve simultaneous saccharification and fermentation of lignocellulosic materials. However, successes in these endeavors were modest, as demonstrated by the relatively low ethanol titers and the limited ability of the engineered yeast strains to grow using cellulosic materials as the sole carbon source. Recently, substantial enhancements to the breakdown of cellulosic substrates have been observed when lytic polysaccharide monooxygenases (LPMOs) were added to traditional cellulase cocktails. LPMOs are repor

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Vu, Nguyen Thi-Hanh, Tung Ngoc Quach, Xuan Thi-Thanh Dao, et al. "A genomic perspective on the potential of termite-associated Cellulosimicrobium cellulans MP1 as producer of plant biomass-acting enzymes and exopolysaccharides." PeerJ 9 (July 28, 2021): e11839. http://dx.doi.org/10.7717/peerj.11839.

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Background Lignocellulose is a renewable and enormous biomass resource, which can be degraded efficiently by a range of cocktails of carbohydrate-active enzymes secreted by termite gut symbiotic bacteria. There is an urgent need to find enzymes with novel characteristics for improving the conversion processes in the production of lignocellulosic-based products. Although various studies dedicated to the genus Cellulosimicrobium as gut symbiont, genetic potential related to plant biomass-acting enzymes and exopolysaccharides production has been fully untapped to date. Methods The cellulolytic ba

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Vintila, Teodor, Vasile Daniel Gherman, Nicolae Popa, Dumitru Popescu, Carmen Buzatu, and Marilena Motoc. "Influence of Enzymatic Cocktails on Conversion of Agricultural Lignocellulose to Fermentable Sugars." Revista de Chimie 68, no. 2 (2017): 373–77. http://dx.doi.org/10.37358/rc.17.2.5456.

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Agricultural lignocellulosic biomass is regarded as an important source of biofuels, especially bioethanol and biohydrogen. The following aspects have been studied: the effect of type of substrate used in production of cellulolytic enzymes, the activity of several enzymatic cocktails used to hydrolyse three types of agricultural biomass and the influence of provenience of enzymatic cocktails on sugars yields in the hydrolysis process. Fungi investigated in this study (T. longibrachiatum DSM 769) release higher titter of enzymes when raw, unpretreated agriculture residual biomass is used as sub

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Balázs, György L., and Olivér Czoboly. "Fibre Cocktail to Improve Fire Resistance." Key Engineering Materials 711 (September 2016): 480–87. http://dx.doi.org/10.4028/www.scientific.net/kem.711.480.

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Favourable experience with fibre reinforced concrete (FRC) resulted in its increasing use worldwide. The properties of fibre reinforced concrete are mostly influenced by the type and the amount of fibres. Our experimental study was directed to the possible improvements of the residual flexural strength and the properties of concrete exposed to high temperatures with different fibre cocktails including steel, micro polymer or cellulose fibres. The influence of type and amount of fibres on residual flexural strength in cold state were tested after 300, 500 or 800 °C temperature loading.

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Adsul, Mukund, Bhawna Sharma, Reeta Rani Singhania, et al. "Blending of cellulolytic enzyme preparations from different fungal sources for improved cellulose hydrolysis by increasing synergism." RSC Adv. 4, no. 84 (2014): 44726–32. http://dx.doi.org/10.1039/c4ra08129c.

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Dorfel, Daniela, Barbara Beck, Christiane Geiger, et al. "The TLR7/8 Ligand R848 Is Superior In Generation of Monocyte Derived Dendritic Cells From AML Patients for the Induction of Potent T and NK Cell Immune Responses." Blood 116, no. 21 (2010): 2195. http://dx.doi.org/10.1182/blood.v116.21.2195.2195.

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Abstract Abstract 2195 Introduction: Therapeutic vaccination with dendritic cells (DC) is currently considered as an investigational therapy in acute myeloid leukemia (AML) for eradication of minimal residual disease (MRD). Dendritic cells derived from autologous peripheral blood monocytes have been tested as cellular adjuvants for therapeutic vaccination of malignancies and proven feasibility and safety, but overall clinical response rates remain very low. The vast majority of DCs used for clinical trials were differentiated with a standard maturation cocktail composed of the cytokines TNF-a,

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Ali, Arslan, Bernhard Ellinger, Sophie C. Brandt, et al. "Genome and Secretome Analysis of Staphylotrichum longicolleum DSM105789 Cultured on Agro-Residual and Chitinous Biomass." Microorganisms 9, no. 8 (2021): 1581. http://dx.doi.org/10.3390/microorganisms9081581.

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Staphylotrichum longicolleum FW57 (DSM105789) is a prolific chitinolytic fungus isolated from wood, with a chitinase activity of 0.11 ± 0.01 U/mg. We selected this strain for genome sequencing and annotation, and compiled its growth characteristics on four different chitinous substrates as well as two agro-industrial waste products. We found that the enzymatic mixture secreted by FW57 was not only able to digest pre-treated sugarcane bagasse, but also untreated sugarcane bagasse and maize leaves. The efficiency was comparable to a commercial enzymatic cocktail, highlighting the potential of th

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Mafa, Mpho S., Samkelo Malgas, Abhishek Bhattacharya, Konanani Rashamuse, and Brett I. Pletschke. "The Effects of Alkaline Pretreatment on Agricultural Biomasses (Corn Cob and Sweet Sorghum Bagasse) and Their Hydrolysis by a Termite-Derived Enzyme Cocktail." Agronomy 10, no. 8 (2020): 1211. http://dx.doi.org/10.3390/agronomy10081211.

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Sweet sorghum bagasse (SSB) and corncob (CC) have been identified as promising feedstocks for the production of second-generation biofuels and other value-added chemicals. In this study, lime (Ca(OH)2) and NaOH pretreatment efficacy for decreasing recalcitrance from SSB and CC was investigated, and subsequently, the pretreated biomass was subjected to the hydrolytic action of an in-house formulated holocellulolytic enzyme cocktail (HEC-H). Compositional analysis revealed that SSB contained 29.34% lignin, 17.75% cellulose and 16.28% hemicellulose, while CC consisted of 22.51% lignin, 23.58% cel

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Huron, Maïté, Damien Hudebine, Nicolas Lopes Ferreira, and Dominique Lachenal. "Mechanistic modeling of enzymatic hydrolysis of cellulose integrating substrate morphology and cocktail composition." Biotechnology and Bioengineering 113, no. 5 (2015): 1011–23. http://dx.doi.org/10.1002/bit.25873.

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Anand, S. B., K. N. Kodumudi, M. V. Reddy, and P. Kaliraj. "A combination of two Brugia malayi filarial vaccine candidate antigens (BmALT-2 and BmVAH) enhances immune responses and protection in jirds." Journal of Helminthology 85, no. 4 (2011): 442–52. http://dx.doi.org/10.1017/s0022149x10000799.

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AbstractIn this study filarial recombinant protein or DNA vaccine constructs encoding BmALT-2 and BmVAH as single or as cocktail antigens were evaluated. Male jirds were immunized intramuscularly with DNA vaccine constructs or were immunized intraperitoneally with protein vaccine. The single and bicistronic DNA constructs induced substantial interferon-γ responses in spleen cells; antigen-specific responses were higher following immunization with the bicistronic cocktail construct and evoked a significant protective response of 57% in jirds challenged with Brugia malayi that was similar in the

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