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Journal articles on the topic 'Cellule hep-2'

Author: Grafiati

Published: 4 June 2021

Last updated: 5 February 2022

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1

Daves, Massimo, Chiara Bonaguri, Silvestro Scala, et al. "Inusuale pattern immunofluoroscopico su cellule HEp-2." La Rivista Italiana della Medicina di Laboratorio - Italian Journal of Laboratory Medicine 8, no. 1 (2012): 54–57. http://dx.doi.org/10.1007/s13631-012-0041-4.

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Krebs, M., B. Evrard, T. Espinouze, C. Bonnafoux, N. Fabien, and A. Tridon. "Fluorescence cytoplasmique dense sur cellule Hep-2 : ne pas passer à côté des anti-PL." Pathologie Biologie 56, no. 1 (2008): 15–20. http://dx.doi.org/10.1016/j.patbio.2007.08.010.

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3

Renier, Gilles, and Céline Beauvillain. "Aspects de fluorescence cytoplasmiquesur cellules HEp-2." Revue Francophone des Laboratoires 2006, no. 384 (2006): 43–50. http://dx.doi.org/10.1016/s1773-035x(06)80303-5.

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4

Magdelaine, Charlotte, Céline Vigneron, and Danielle Degenne. "Aspects des anticorps antinucléaires surles cellules HEp-2." Revue Francophone des Laboratoires 2006, no. 384 (2006): 33–41. http://dx.doi.org/10.1016/s1773-035x(06)80302-3.

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5

Goetz, Joëlle. "Conduite à tenir devant une fluorescence cytoplasmique des cellules HEp-2." Revue Francophone des Laboratoires 2006, no. 384 (2006): 5–10. http://dx.doi.org/10.1016/s1773-035x(06)80315-1.

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6

Parvaze, P. Suhail, and S. Ramakrishnan. "Extraction of Multiple Cellular Objects in HEp-2 Images using LS Segmentation." IEIE Transactions on Smart Processing & Computing 6, no. 6 (2017): 401–8. http://dx.doi.org/10.5573/ieiespc.2017.6.6.401.

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TESAR, JOSEPH T., and JAMES ARMSTRONG. "Effect of Adenovirus Infection on Cellular Localization of Small Ribonucleoproteins in HEP-2 Cells." Annals of the New York Academy of Sciences 494, no. 1 Third Colloqu (1987): 278–79. http://dx.doi.org/10.1111/j.1749-6632.1987.tb29546.x.

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8

De Melo, M. A., G. Gabbiani, and J. C. Pechère. "Cellular events and intracellular survival of Campylobacter jejuni during infection of HEp-2 cells." Infection and Immunity 57, no. 7 (1989): 2214–22. http://dx.doi.org/10.1128/iai.57.7.2214-2222.1989.

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9

Machado, Aline Helena Araujo, Karen C. M. Moraes, Cristina Pacheco Soares, Milton Beltrame Junior, and Newton Soares da Silva. "Cellular Changes After Photodynamic Therapy on HEp-2 Cells Using the New ZnPcBr8 Phthalocyanine." Photomedicine and Laser Surgery 28, S1 (2010): S—143—S—149. http://dx.doi.org/10.1089/pho.2009.2561.

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jerbi, A., H. Hend, S. Marzouk, et al. "L’absence de fluorescence cytoplasmique des cellules HEp-2 n’est pas rare en présence d’anticorps des myopathies inflammatoires." La Revue de Médecine Interne 42 (June 2021): A186. http://dx.doi.org/10.1016/j.revmed.2021.03.186.

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Journal, Baghdad Science. "Evaluation of Transfected HEP-2 Cell Line Using ß-Galactosidase Reporter Assay System." Baghdad Science Journal 8, no. 3 (2011): 728–35. http://dx.doi.org/10.21123/bsj.8.3.728-735.

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Liposome-mediated transfection of cancer cells provide a valuable experimental technique to study cellular gene expression and may also be adapted for gene therapy studies. However, the widely recognized advantage of liposome-mediated transfection is high efficiency. Therefore, this study were performed to optimize transfection techniques in human larynx carcinoma cell line Hep-2 using the commercial synthetic lipid TransFast™ Reagent and monitoring the expression efficiency by using the pSV-?-galactosidase Control Vector which encoded ?-galactosidase, maximum transfection efficiency were achi

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12

Lima, Eleonidas Moura, Jorge Dores Rissino, Adriana Costa Guimarães, et al. "Drifter technique: a new method to obtain metaphases in Hep-2 cell line cultures." Brazilian Archives of Biology and Technology 48, no. 4 (2005): 537–40. http://dx.doi.org/10.1590/s1516-89132005000500005.

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The Hep-2 cell line is derived from laryngeal carcinoma cells and is often utilized as a model in carcinogenesis and mutagenesis tests. To evaluate the proliferative potential of this line, we developed a cytogenetic methodology (drifter technique) to obtain metaphases from cells that loose cellular adhesion when they underwent mitosis in culture. By this procedure, 2000 cells were counted, resulting in a mitotic index (MI) of 22.2%. Although this MI was not statistically different from the one obtained using either a classical cytogenetic method or a cell synchronization technique, the drifte

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13

Vununu, Caleb, Suk-Hwan Lee, and Ki-Ryong Kwon. "A Strictly Unsupervised Deep Learning Method for HEp-2 Cell Image Classification." Sensors 20, no. 9 (2020): 2717. http://dx.doi.org/10.3390/s20092717.

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Classifying the images that portray the Human Epithelial cells of type 2 (HEp-2) represents one of the most important steps in the diagnosis procedure of autoimmune diseases. Performing this classification manually represents an extremely complicated task due to the heterogeneity of these cellular images. Hence, an automated classification scheme appears to be necessary. However, the majority of the available methods prefer to utilize the supervised learning approach for this problem. The need for thousands of images labelled manually can represent a difficulty with this approach. The first co

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14

Wang, Shu, Xiaoqun Liu, Tiankui Qiao, and Qi Zhang. "Radiosensitization by CpG ODN7909 in an epidermoid laryngeal carcinoma Hep-2 cell line." Journal of International Medical Research 45, no. 6 (2017): 2009–22. http://dx.doi.org/10.1177/0300060517728634.

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Objective To evaluate the radiosensitivity effect of CpG oligodeoxyribonucleotide (ODN) 7909 on human epidermoid cancer strain-2 (Hep-2) cells in vitro and discuss the potential for improved radiotherapy treatment in patients with laryngeal squamous cell carcinoma. Methods Toll-like receptor ( TLR) 9 expression was assessed in Hep-2 cells using Western blots and reverse transcription polymerase chain reaction. Cell Counting Kit-8 was used to detect Hep-2 cell viability at 24 and 48 h following treatment with different CpG ODN7909 concentrations. Cellular colonization was evaluated using micros

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15

Devanathan, Kanchana, Kavitha Ganesan, and Ramakrishnan Swaminathan. "An automated classification of HEp-2 cellular shapes using Bag-of-keypoint features and Ant Colony Optimization." Biocybernetics and Biomedical Engineering 41, no. 2 (2021): 376–90. http://dx.doi.org/10.1016/j.bbe.2021.02.004.

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16

Vununu, Caleb, Suk-Hwan Lee, Oh-Jun Kwon, and Ki-Ryong Kwon. "A Dynamic Learning Method for the Classification of the HEp-2 Cell Images." Electronics 8, no. 8 (2019): 850. http://dx.doi.org/10.3390/electronics8080850.

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The complete analysis of the images representing the human epithelial cells of type 2, commonly referred to as HEp-2 cells, is one of the most important tasks in the diagnosis procedure of various autoimmune diseases. The problem of the automatic classification of these images has been widely discussed since the unfolding of deep learning-based methods. Certain datasets of the HEp-2 cell images exhibit an extreme complexity due to their significant heterogeneity. We propose in this work a method that tackles specifically the problem related to this disparity. A dynamic learning process is cond

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Barlak, Neslisah, Fatma Sanli, Ozel Capik, et al. "Metformin Treatment Sensitizes Human Laryngeal Cancer Cell Line Hep- 2 to 5-Fluorouracil." Clinical Cancer Drugs 7, no. 1 (2020): 16–24. http://dx.doi.org/10.2174/2212697x06666190906165309.

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Background: Larynx cancer (LCa) is the most common head and neck cancer and accounts for 1-2.5% of all human cancers worldwide. Metformin, an oral anti-diabetic drug, has been recently shown to have anti-cancer activity in various cancer types, and there are several studies in the literature pointing to its potential to sensitize cancer cells to chemotherapeutic drugs. Objective: This study was aimed at exploring the anti-cancer effects of metformin alone or in combination with 5-fluorouracil (5-FU) on Hep-2 cells. Methods: The effects of metformin and/or 5-FU on the proliferative, clonogenic,

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18

Kato, Akihisa, Yoshitaka Hirohata, Jun Arii, and Yasushi Kawaguchi. "Phosphorylation of Herpes Simplex Virus 1 dUTPase Upregulated Viral dUTPase Activity To Compensate for Low Cellular dUTPase Activity for Efficient Viral Replication." Journal of Virology 88, no. 14 (2014): 7776–85. http://dx.doi.org/10.1128/jvi.00603-14.

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ABSTRACTWe recently reported that herpes simplex virus 1 (HSV-1) protein kinase Us3 phosphorylated viral dUTPase (vdUTPase) at serine 187 (Ser-187) to upregulate its enzymatic activity, which promoted HSV-1 replication in human neuroblastoma SK-N-SH cells but not in human carcinoma HEp-2 cells. In the present study, we showed that endogenous cellular dUTPase activity in SK-N-SH cells was significantly lower than that in HEp-2 cells and that overexpression of cellular dUTPase in SK-N-SH cells increased the replication of an HSV-1 mutant with an alanine substitution for Ser-187 (S187A) in vdUTPa

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Vununu, Caleb, Suk-Hwan Lee, and Ki-Ryong Kwon. "A Classification Method for the Cellular Images Based on Active Learning and Cross-Modal Transfer Learning." Sensors 21, no. 4 (2021): 1469. http://dx.doi.org/10.3390/s21041469.

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In computer-aided diagnosis (CAD) systems, the automatic classification of the different types of the human epithelial type 2 (HEp-2) cells represents one of the critical steps in the diagnosis procedure of autoimmune diseases. Most of the methods prefer to tackle this task using the supervised learning paradigm. However, the necessity of having thousands of manually annotated examples constitutes a serious concern for the state-of-the-art HEp-2 cells classification methods. We present in this work a method that uses active learning in order to minimize the necessity of annotating the majority

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20

Joglekar, Manali V., Sanjay Khandelwal, Mortimer Poncz, Lubica Rauova, and Gowthami M. Arepally. "Understanding the Underlying Immune Response in HIT: Uptake of PF4/Heparin Complexes By Monocytes & Dendritic Cells." Blood 124, no. 21 (2014): 4197. http://dx.doi.org/10.1182/blood.v124.21.4197.4197.

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Abstract Platelet Factor 4 (PF4), a strongly positive-charged small protein, and the negatively-charged polymer heparin (Hep) form ultra-large complexes (ULCs) that have several unusual features including their remarkable stability and their ability to elicit a robust antibody response in vivo (Rauova L; Blood 2005 and Suvarna S, Blood 2005). Recently, we and others have shown that similar complexes of another positively charged protein, protamine sulfate with heparin can also lead to a clinically relevant immune response. To better understand the cellular basis for PF4/Hep antibody formation,

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21

Galvan, Veronica, Renato Brandimarti, Joshua Munger, and Bernard Roizman. "Bcl-2 Blocks a Caspase-Dependent Pathway of Apoptosis Activated by Herpes Simplex Virus 1 Infection in HEp-2 Cells." Journal of Virology 74, no. 4 (2000): 1931–38. http://dx.doi.org/10.1128/jvi.74.4.1931-1938.2000.

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ABSTRACT Earlier reports have shown that herpes simplex virus 1 (HSV-1) mutants induce programmed cell death and that wild-type virus blocks the execution of the cell death program triggered by expression of viral genes, by the Fas and tumor necrosis factor pathways, or by nonspecific stress agents. In particular, an earlier report from this laboratory showed that the mutant virus d120 lacking the genes encoding infected cell protein 4 (ICP4), the major regulatory protein of the virus, induces a caspase-3-independent pathway of apoptosis in human SK-N-SH cells. Here we report that the pathway

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22

Rudolf, Emil, and Miroslav Červinka. "Zinc pyrithione induces cellular stress signaling and apoptosis in Hep-2 cervical tumor cells: the role of mitochondria and lysosomes." BioMetals 23, no. 2 (2010): 339–54. http://dx.doi.org/10.1007/s10534-010-9302-8.

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23

Hanata, Kyoshi, Noriko Yamaguchi, Kiwamu Yoshikawa, et al. "Soluble EMMPRIN (extra-cellular matrix metalloproteinase inducer) stimulates the migration of HEp-2 human laryngeal carcinoma cells, accompanied by increased MMP-2 production in fibroblasts." Archives of Histology and Cytology 70, no. 5 (2007): 267–77. http://dx.doi.org/10.1679/aohc.70.267.

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24

Lapaillerie, Delphine, Cathy Charlier, Henrique S. Fernandes, et al. "In Silico, In Vitro and In Cellulo Models for Monitoring SARS-CoV-2 Spike/Human ACE2 Complex, Viral Entry and Cell Fusion." Viruses 13, no. 3 (2021): 365. http://dx.doi.org/10.3390/v13030365.

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the etiologic agent responsible for the recent coronavirus disease 2019 (COVID-19) pandemic. Productive SARS-CoV-2 infection relies on viral entry into cells expressing angiotensin-converting enzyme 2 (ACE2). Indeed, viral entry into cells is mostly mediated by the early interaction between the viral spike protein S and its ACE2 receptor. The S/ACE2 complex is, thus, the first contact point between the incoming virus and its cellular target; consequently, it has been considered an attractive therapeutic target. To further characte

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25

Nicoud, I. B., C. D. Knox, C. M. Jones, et al. "2-APB protects against liver ischemia-reperfusion injury by reducing cellular and mitochondrial calcium uptake." American Journal of Physiology-Gastrointestinal and Liver Physiology 293, no. 3 (2007): G623—G630. http://dx.doi.org/10.1152/ajpgi.00521.2006.

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Ischemia-reperfusion (I/R) injury is a commonly encountered clinical problem in liver surgery and transplantation. The pathogenesis of I/R injury is multifactorial, but mitochondrial Ca2+ overload plays a central role. We have previously defined a novel pathway for mitochondrial Ca2+ handling and now further characterize this pathway and investigate a novel Ca2+-channel inhibitor, 2-aminoethoxydiphenyl borate (2-APB), for preventing hepatic I/R injury. The effect of 2-APB on cellular and mitochondrial Ca2+ uptake was evaluated in vitro by using 45Ca2+. Subsequently, 2-APB (2 mg/kg) or vehicle

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26

Beyne-Raury, O., S. Arista, A. Godel, et al. "Dépistage des anticorps antinucléaires par immunofluorescence indirecte sur cellules Hep-2 évaluation du rendement diagnostique et conséquences. À propos de 10 000 demandes." La Revue de Médecine Interne 23 (December 2002): 566s. http://dx.doi.org/10.1016/s0248-8663(02)80423-4.

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27

Thammawat, Sutthiwan, Tania A. Sadlon, Peter G. Hallsworth, and David L. Gordon. "Role of Cellular Glycosaminoglycans and Charged Regions of Viral G Protein in Human Metapneumovirus Infection." Journal of Virology 82, no. 23 (2008): 11767–74. http://dx.doi.org/10.1128/jvi.01208-08.

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ABSTRACT Human metapneumovirus (hMPV) is an important cause of lower respiratory tract disease, particularly in infants and young children. hMPV has two major glycoproteins, G and F, which are responsible for virus attachment and membrane fusion, respectively. We investigated the role of cellular glycosaminoglycans (GAGs) and G protein in hMPV infection. The pretreatment of hMPV with soluble heparin markedly inhibited the infection of HEp-2 cells. Recombinant G protein, comprising the extracellular domain of G, bound to heparin-agarose columns and also to HEp-2 cells. hMPV infection and G prot

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Peri, Piritta, Veijo Hukkanen, Kristiina Nuutila, Pekka Saukko, Magnus Abrahamson, and Tytti Vuorinen. "The cysteine protease inhibitors cystatins inhibit herpes simplex virus type 1-induced apoptosis and virus yield in HEp-2 cells." Journal of General Virology 88, no. 8 (2007): 2101–5. http://dx.doi.org/10.1099/vir.0.82990-0.

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The role of cystatins in herpes simplex virus (HSV)-induced apoptosis and viral replication has been studied. Human epithelial (HEp-2) cells infected with wild-type HSV-1 (F), with a deletion virus lacking the anti-apoptotic gene Us3 (R7041) or with a deletion virus lacking the anti-apoptotic genes Us3 and ICP4 (d120) were treated with cystatin A, C or D. Cells and culture media were studied at different time points for replicating HSV-1 and for apoptosis. Cystatins C and D inhibited the yield of replicative HSV-1 significantly in HEp-2 cells. In addition, cystatin D inhibited R7041 and d120 v

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Vötsch, Désirée, Maren Willenborg, Walter M. R. Oelemann, Graham Brogden, and Peter Valentin-Weigand. "Membrane Binding, Cellular Cholesterol Content and Resealing Capacity Contribute to Epithelial Cell Damage Induced by Suilysin of Streptococcus suis." Pathogens 9, no. 1 (2019): 33. http://dx.doi.org/10.3390/pathogens9010033.

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Streptococcus (S.) suis is a major cause of economic losses in the pig industry worldwide and is an emerging zoonotic pathogen. One important virulence-associated factor is suilysin (SLY), a toxin that belongs to the family of cholesterol-dependent pore-forming cytolysins (CDC). However, the precise role of SLY in host–pathogen interactions is still unclear. Here, we investigated the susceptibility of different respiratory epithelial cells to SLY, including immortalized cell lines (HEp-2 and NPTr cells), which are frequently used in in vitro studies on S. suis virulence mechanisms, as well as

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Boyoglu, Cemil, Qingwen He, Gerold Willing, et al. "Microscopic Studies of Various Sizes of Gold Nanoparticles and Their Cellular Localizations." ISRN Nanotechnology 2013 (July 28, 2013): 1–13. http://dx.doi.org/10.1155/2013/123838.

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Gold nanoparticles (GNPs) are widely used in biological and clinical applications due to their favorable chemical and optical properties. GNPs can be used for drug delivery to targeted cells. In addition, GNPs serve as ideal probes for biological and cell imaging applications. Recent studies indicate that the size diversity of GNPs plays an important role in targeting cellular components for biomedical applications. In this study, we conducted a series of studies using different sizes of gold nanoparticles, including 3, 10, 25, and 50 nm, to determine the effect of size variations on their int

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31

Chu, Charles C., Xiao Bo Wang, Katerina Hatzi, et al. "B-CLL Antibodies Comprised of Stereotypic VH1-69, D3-16, and JH3 Rearrangements Immunoprecipitate Cellular Protein(s)." Blood 108, no. 11 (2006): 2816. http://dx.doi.org/10.1182/blood.v108.11.2816.2816.

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Abstract B-cell chronic lymphocytic leukemia (B-CLL) is typically due to a clonal expansion of B-lymphocytes that can be divided into two classes based on the mutation status of the variable region of the antibody produced by the leukemic clone. Mutated B-CLL (antibody with ≥ 2% mutation) is associated with longer survival than unmutated B-CLL. Segregation of patients based on antibody mutation suggests a functional role for antigen binding to antibody in this disease. Furthermore, many different subsets of B-CLL antibodies have very similar CDR3 sequences, suggesting some common antigen react

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32

Hommel, B., J. L. Charuel, L. Arnaud, et al. "Un aspect particulier en immunofluorescence indirecte sur cellules HEp-2 est hautement évocateur de paludisme et permet de dépister des maladies palustres chroniques dormantes paucisymptomatiques." La Revue de Médecine Interne 34 (June 2013): A40. http://dx.doi.org/10.1016/j.revmed.2013.03.241.

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33

Yoshida, Minoru, Motohiro Tomizawa, Shao-Yong Wu, Gary B. Quistad, and John E. Casida. "Neuropathy Target Esterase of Hen Brain: Active Site Reactions with 2-[octyl-3H]Octyl-4H-1,3,2-Benzodioxaphosphorin 2-Oxide and 2-Octyl-4H-1,3,2-[aryl-3H] Benzodioxaphosphorin 2-Oxide." Journal of Neurochemistry 64, no. 4 (2002): 1680–87. http://dx.doi.org/10.1046/j.1471-4159.1995.64041680.x.

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Koczura, Ryszard, Joanna Mokracka, Sylwia Krzymińska, and Adam Kaznowski. "Virulence properties and integron-associated antibiotic resistance of Klebsiella mobilis strains isolated from clinical specimens." Journal of Medical Microbiology 60, no. 3 (2011): 281–88. http://dx.doi.org/10.1099/jmm.0.024059-0.

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This study examined Klebsiella mobilis isolates cultured from clinical specimens for virulence-associated properties and antibiotic resistance. The strains produced a number of siderophores, including enterobactin, aerobactin and yersiniabactin. All isolates were able to adhere to and invade epithelial cells. They had cytotoxic activity, which caused destruction of human laryngeal epithelial HEp-2 cells and evoked lysis of murine macrophage J774 cells. Analyses of HEp-2 and J774 cellular morphology and DNA fragmentation in the cells showed features typical of cells undergoing apoptosis. Some K

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Gozgit, Joseph M., Geraldine A. Bebernitz, Pankaj Patil, et al. "Effects of a Novel, Selective Jak2 Inhibitor, AZ60, on STAT5 Signaling and Cellular Growth in Jak2 V617F Cell Lines." Blood 110, no. 11 (2007): 3549. http://dx.doi.org/10.1182/blood.v110.11.3549.3549.

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Abstract A role for Jak2 in the etiology of the myeloproliferative diseases (MPDs) was discovered with the identification of a single activating point mutation, V617F, in the pseudokinase domain of JAK2. We have developed a Jak2 inhibitor, AZ60, which inhibits in vitro JAK2 enzyme activity with a Ki of 0.45 nM. AZ60 demonstrates inhibition of STAT5 phosphorylation and proliferation in a Tel-Jak2 engineered cell line with IC50 values of 18 and 23 nM, respectively. To understand the selectivity versus other Jak kinase family members we engineered three additional cell lines containing Tel fusion

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Peterson, Lisa K., Anne E. Tebo, Mark H. Wener, Susan S. Copple, and Marvin J. Fritzler. "Assessment of antinuclear antibodies by indirect immunofluorescence assay: report from a survey by the American Association of Medical Laboratory Immunologists." Clinical Chemistry and Laboratory Medicine (CCLM) 58, no. 9 (2020): 1489–97. http://dx.doi.org/10.1515/cclm-2019-1262.

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AbstractBackgroundThe indirect immunofluorescence assay (IFA) using HEp-2 cell substrates is the preferred method by some for detecting antinuclear antibodies (ANA) as it demonstrates a number of characteristic staining patterns that reflect the cellular components bound as well as semi-quantitative results. Lack of harmonized nomenclature for HEp-2 IFA patterns, subjectivity in interpretation and variability in the number of patterns reported by different laboratories pose significant harmonization challenges. The main objectives of this study were to assess current practice in laboratory ass

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Machado, Aline Helena Araujo, Pacheco Cristina Soares, Newton Soares Da Silva, and Karen C. M. Moraes. "Cellular and molecular studies of the initial process of the photodynamic therapy in HEp-2 cells using LED light source and two different photosensitizers." Cell Biology International 33, no. 7 (2009): 785–95. http://dx.doi.org/10.1016/j.cellbi.2009.04.011.

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38

Sato, Yuka, Akihisa Kato, Yuhei Maruzuru, et al. "Cellular Transcriptional Coactivator RanBP10 and Herpes Simplex Virus 1 ICP0 Interact and Synergistically Promote Viral Gene Expression and Replication." Journal of Virology 90, no. 6 (2016): 3173–86. http://dx.doi.org/10.1128/jvi.03043-15.

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ABSTRACTTo investigate the molecular mechanism(s) by which herpes simplex virus 1 (HSV-1) regulatory protein ICP0 promotes viral gene expression and replication, we screened cells overexpressing ICP0 for ICP0-binding host cell proteins. Tandem affinity purification of transiently expressed ICP0 coupled with mass spectrometry-based proteomics technology and subsequent analyses showed that ICP0 interacted with cell protein RanBP10, a known transcriptional coactivator, in HSV-1-infected cells. Knockdown of RanBP10 in infected HEp-2 cells resulted in a phenotype similar to that observed with the I

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Perez, Aleida, Qing-Xue Li, Pilar Perez-Romero, et al. "A New Class of Receptor for Herpes Simplex Virus Has Heptad Repeat Motifs That Are Common to Membrane Fusion Proteins." Journal of Virology 79, no. 12 (2005): 7419–30. http://dx.doi.org/10.1128/jvi.79.12.7419-7430.2005.

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ABSTRACT We isolated a human cDNA by expression cloning and characterized its gene product as a new human protein that enables entry and infection of herpes simplex virus (HSV). The gene, designated hfl-B5, encodes a type II cell surface membrane protein, B5, that is broadly expressed in human primary tissue and cell lines. It contains a high-scoring heptad repeat at the extracellular C terminus that is predicted to form an α-helix for coiled coils like those in cellular SNAREs or in some viral fusion proteins. A synthetic 30-mer peptide that has the same sequence as the heptad repeat α-helix

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Kaewsaneha, Chariya, Kulachart Jangpatarapongsa, Tienrat Tangchaikeeree, Duangporn Polpanich, and Pramuan Tangboriboonrat. "Fluorescent chitosan functionalized magnetic polymeric nanoparticles: Cytotoxicity and in vitro evaluation of cellular uptake." Journal of Biomaterials Applications 29, no. 5 (2014): 761–68. http://dx.doi.org/10.1177/0885328214540349.

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Nanoparticles possessing magnetic and fluorescent properties were fabricated by the covalent attachment of fluorescein isothiocyanate onto magnetic polymeric nanoparticles functionalized by chitosan. The synthesized magnetic polymeric nanoparticles-chitosan/fluorescein isothiocyanate were successfully used for labeling the living organ and blood-related cancer cells, i.e., HeLa, Hep G2, and K562 cells. The cytotoxicity test of nanoparticles at various incubation times indicated the high cell viability (>90%) without morphological change. The confocal microscopy revealed that they could pass

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Advani, Sunil J., Ryan Hagglund, Ralph R. Weichselbaum, and Bernard Roizman. "Posttranslational Processing of Infected Cell Proteins 0 and 4 of Herpes Simplex Virus 1 Is Sequential and Reflects the Subcellular Compartment in Which the Proteins Localize." Journal of Virology 75, no. 17 (2001): 7904–12. http://dx.doi.org/10.1128/jvi.75.17.7904-7912.2001.

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ABSTRACT The herpes simplex virus 1 (HSV-1) infected cell proteins 0 and 4 (ICP0 and ICP4) are multifunctional proteins extensively posttranscriptionally processed by both cellular and viral enzymes. We examined by two-dimensional separations the posttranslational forms of ICP0 and ICP4 in HEp-2 cells and in human embryonic lung (HEL) fibroblasts infected with wild-type virus, mutant R325, lacking the sequences encoding the US1.5 protein and the overlapping carboxyl-terminal domain of ICP22, or R7914, in which the aspartic acid 199 of ICP0 was replaced by alanine. We report the following (i) B

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Blumenthal, Britta, Claudia Hoffmann, Klaus Aktories, Steffen Backert, and Gudula Schmidt. "The Cytotoxic Necrotizing Factors from Yersinia pseudotuberculosis and from Escherichia coli Bind to Different Cellular Receptors but Take the Same Route to the Cytosol." Infection and Immunity 75, no. 7 (2007): 3344–53. http://dx.doi.org/10.1128/iai.01937-06.

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ABSTRACT The cytotoxic necrotizing factors CNF1 and CNF2 produced by pathogenic Escherichia coli strains and CNFY of Yersinia pseudotuberculosis constitutively activate small GTPases of the Rho family. They deamidate a glutamine (Gln63 in RhoA), which is crucial for GTP hydrolysis. CNF1 and CNFY exhibit 61% identity on the amino acid level, with equal distribution over the whole molecule. Although the two toxins are homologous in the receptor binding domain, we show that they bind to different cellular receptors. CNFY does not enter Caco-2 and CHO-K1 cells, which are responsive to CNF1. In con

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Bossert, Birgit, and Karl-Klaus Conzelmann. "Respiratory Syncytial Virus (RSV) Nonstructural (NS) Proteins as Host Range Determinants: a Chimeric Bovine RSV with NS Genes from Human RSV Is Attenuated in Interferon-Competent Bovine Cells." Journal of Virology 76, no. 9 (2002): 4287–93. http://dx.doi.org/10.1128/jvi.76.9.4287-4293.2002.

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ABSTRACT Bovine respiratory syncytial virus (BRSV) escapes from cellular responses to alpha/beta interferon (IFN-α/β) by a concerted action of the two viral nonstructural proteins, NS1 and NS2. Here we show that the NS proteins of human RSV (HRSV) are also able to counteract IFN responses and that they have the capacity to protect replication of an unrelated rhabdovirus. Even combinations of BRSV and HRSV NS proteins showed a protective activity, suggesting common mechanisms and cellular targets of HRSV and BRSV NS proteins. Although able to cooperate, NS proteins from BRSV and HRSV showed dif

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Heimlich, B., H. Schwegler, and W. E. Crusio. "Hippocampal Variation between the Inbred Mouse Strains C3H/HeJ and DBA/2: A Quantitative-genetic Analysis." Journal of Neurogenetics 2, no. 6 (1985): 389–401. http://dx.doi.org/10.3109/01677068509101425.

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Pham, F., J. L. Charuel, P. Ghillani-Dalbin, et al. "L’absence de fluorescence cytoplasmique dans les cellules HEp-2 est rare en présence d’anticorps anti-synthétase ou d’anti-SRP au cours des myopathies inflammatoires et des pneumopathies interstitielles." La Revue de Médecine Interne 34 (June 2013): A63—A64. http://dx.doi.org/10.1016/j.revmed.2013.03.284.

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Havekes, L. M., E. C. M. de Wit, and H. M. G. Princen. "Cellular free cholesterol in Hep G2 cells is only partially available for down-regulation of low-density-lipoprotein receptor activity." Biochemical Journal 247, no. 3 (1987): 739–46. http://dx.doi.org/10.1042/bj2470739.

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We have previously shown that in Hep G2 cells and human hepatocytes, as compared with fibroblasts, the low-density lipoprotein (LDL) receptor activity is only weakly down-regulated after incubation of the cells with LDL, whereas incubation with high-density lipoproteins (HDL) of density 1.16-1.20 g/ml (heavy HDL) strongly increased the LDL-receptor activity. To elucidate this difference between hepatocytes and fibroblasts, we studied the cellular cholesterol homoeostasis in relation to the LDL-receptor activity in Hep G2 cells. (1) Interrupting the cholesteryl ester cycle by inhibiting acyl-Co

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Villaseca, Jorge M., Fernando Navarro-García, Guillermo Mendoza-Hernández, James P. Nataro, Alejandro Cravioto, and Carlos Eslava. "Pet Toxin from Enteroaggregative Escherichia coli Produces Cellular Damage Associated with Fodrin Disruption." Infection and Immunity 68, no. 10 (2000): 5920–27. http://dx.doi.org/10.1128/iai.68.10.5920-5927.2000.

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ABSTRACT Pet toxin is a serine protease from enteroaggregativeEscherichia coli which has been described as causing enterotoxic and cytotoxic effects. In this paper we show that Pet produces spectrin and fodrin (nonerythroid spectrin) disruption. Using purified erythrocyte membranes treated with Pet toxin, we observed degradation of α- and β-spectrin chains; this effect was dose and time dependent, and a 120-kDa protein fraction was observed as a breakdown product. Spectrin degradation and production of the 120-kDa subproduct were confirmed using specific antibodies against the α- and β-spectri

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Aleo, M. D., and R. G. Schnellmann. "Regulation of glycolytic metabolism during long-term primary culture of renal proximal tubule cells." American Journal of Physiology-Renal Physiology 262, no. 1 (1992): F77—F85. http://dx.doi.org/10.1152/ajprenal.1992.262.1.f77.

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Adequate oxygenation was a major factor regulating the induction of glycolytic metabolism in primary cultures of rabbit renal proximal tubule cells during short-term (less than 1 day) and long-term (1-7 day) culture. As measured by cellular lactate content, glucose consumption, and lactate dehydrogenase activity, less glycolytic metabolism was induced in cultured cells that were constantly aerated than in cells that were held stationary. When oxidative metabolism is supported by providing 2-10 mM heptanoate (HEP) as a metabolic substrate glycolytic metabolism further decreased during short-ter

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Boyd, L. R., E. V. Ariztia, and D. A. Fishman. "Activation of MMP-2 by endometrial cancer cells in vitro is enhanced by LPA." Journal of Clinical Oncology 25, no. 18_suppl (2007): 16047. http://dx.doi.org/10.1200/jco.2007.25.18_suppl.16047.

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16047 Background: Matrix metalloproteinases (MMPs) are responsible for the breakdown of extracellular matrix components, and are important in the metastatic phenotype. HEC-1A is an endometrial cancer cell line, derived from a moderately-differentiated tumor. These cells do not produce MMP-2, an important mediator of invasive behavior. We sought to: evaluate if HEC-1A cells have the capacity to activate proMMP-2 if given the proenzyme exogenously; and determine if the addition of lysophosphatidic acid (LPA), a known activator of MMPs in other cancers, would further enhance this conversion. Meth

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Han, Jin-Young, Sara A. Miller, Teresa M. Wolfe, Hoda Pourhassan, and Keith R. Jerome. "Cell Type-Specific Induction and Inhibition of Apoptosis by Herpes Simplex Virus Type 2 ICP10." Journal of Virology 83, no. 6 (2008): 2765–69. http://dx.doi.org/10.1128/jvi.02088-08.

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ABSTRACT Herpes simplex virus (HSV) inhibits apoptosis induced by external stimuli in epithelial cells. In contrast, apoptosis is the primary outcome in HSV-infected lymphocytes. Here, we show that HSV type 2 (HSV-2) gene expression appears to be necessary for the induction of apoptosis in Jurkat cells, a T-cell leukemia line. HSV-2 ICP10 gene expression is sufficient to induce apoptosis in Jurkat cells, while its expression protects epithelial HEp-2 cells from apoptosis triggered by cycloheximide and tumor necrosis factor alpha. Thus, the effect of HSV-2 gene expression on the cellular apopto

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