Relevant bibliographies by topics / Cellules – Culture – Technique

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers

Academic literature on the topic 'Cellules – Culture – Technique'

Author: Grafiati
Published: 4 June 2021
Last updated: 12 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Cellules – Culture – Technique.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Cellules – Culture – Technique"

1

Johnson, L. G., K. G. Dickman, K. L. Moore, L. J. Mandel, and R. C. Boucher. "Enhanced Na+ transport in an air-liquid interface culture system." American Journal of Physiology-Lung Cellular and Molecular Physiology 264, no. 6 (June 1, 1993): L560—L565. http://dx.doi.org/10.1152/ajplung.1993.264.6.l560.

Full text
Abstract:
Use of the air-liquid interface culture technique has produced improved morphological differentiation of rodent, canine, and human tracheal epithelia. We have investigated the effect of this culture technique on ion transport activities of cultured canine bronchial epithelia. These cells were isolated from excised airways by enzymatic digestion and plated on permeable collagen membrane substrates. All cultures were maintained utilizing standard culture techniques, by bathing both apical and basolateral sides with hormone supplemented, serum-free media until confluent (days 4–6). Half of the cultures were converted to air-liquid interface cultures (ALIC) by gentle aspiration of the apical medium and half were continued under standard technique culture (STC) conditions. After three additional days, preparations cultured under both conditions were mounted in modified Ussing chambers where bioelectric properties were measured under short-circuit conditions. Mean short-circuit current (Isc) was significantly greater in ALIC (-91.3 +/- 7.84 microA/cm2) than in STC (-54.8 +/- 5.03 microA/cm2). The sodium channel blocker, amiloride, reduced Isc by 68.4 +/- 5.0% in STC and by 84.8 +/- 3.0% in ALIC. 22Na and 36Cl fluxes confirmed the presence of enhanced sodium absorption in ALIC when compared with STC. The depth of the apical fluid, measured by microelectrodes during ALIC, was approximately 15 microns. Studies of cellular metabolism demonstrated a shift in metabolism from an anaerobic to an oxidative pattern in ALIC. This change in the pattern of metabolism suggests that the ALIC technique enhanced sodium transport in canine bronchial epithelia by increasing oxygen delivery to the epithelium.
APA, Harvard, Vancouver, ISO, and other styles
2

Goldsmith, S. J., M. A. Thomas, and C. Gries. "A New Technique for Photobiont Culturing and Manipulation." Lichenologist 29, no. 6 (November 1997): 559–69. http://dx.doi.org/10.1006/lich.1997.0108.

Full text
Abstract:
AbstractComparisons of whole-lichen physiology to the respective photobionts have often been unclear due to inherent differences in isolated photobiont culturing techniques. The use of 13-mm-diameter cellulose-acetate discs allows photobiont cultures access to nutrient agar medium, while improving ease of manipulation and distinct separation from the agar. Adequate culture growth for experimentation is reached in approximately three weeks, a time comparable to standard nutrient agar and liquid cultures. These discs are then available for use in a variety of manipulative techniques. Chlorophyll determination of an entire algal disc culture is obtainable because the discs readily dissolve in dimethylsulphoxide (DMSO), with no interference in the 400–700 nm range. Photosynthesis and respiration may be measured with standard gas exchange equipment. Photobiont discs allow for fumigation in the gas phase with no increase in external ⊂pH reported to occur during gaseous fumigations in liquid media. The disc system is also useful for fluorescence studies. Trebouxia erici cultures exhibited a CO⊂2 gas exchange on a gram dry weight basis similar to whole lichen systems. The ease with which photobionts can be cultured and manipulated using this system allows for expanded experimentation and comparisons.
APA, Harvard, Vancouver, ISO, and other styles
3

Park, Yong H., Joshua D. Snook, Iris Zhuang, Guofu Shen, and Benjamin J. Frankfort. "Optimized culture of retinal ganglion cells and amacrine cells from adult mice." PLOS ONE 15, no. 12 (December 7, 2020): e0242426. http://dx.doi.org/10.1371/journal.pone.0242426.

Full text
Abstract:
Cell culture is widely utilized to study the cellular and molecular biology of different neuronal cell populations. Current techniques to study enriched neurons in vitro are primarily limited to embryonic/neonatal animals and induced pluripotent stem cells (iPSCs). Although the use of these cultures is valuable, the accessibility of purified primary adult neuronal cultures would allow for improved assessment of certain neurological diseases and pathways at the cellular level. Using a modified 7-step immunopanning technique to isolate for retinal ganglion cells (RGCs) and amacrine cells (ACs) from adult mouse retinas, we have successfully developed a model of neuronal culture that maintains for at least one week. Isolations of Thy1.2+ cells are enriched for RGCs, with the isolation cell yield being congruent to the theoretical yield of RGCs in a mouse retina. ACs of two different populations (CD15+ and CD57+) can also be isolated. The populations of these three adult neurons in culture are healthy, with neurite outgrowths in some cases greater than 500μm in length. Optimization of culture conditions for RGCs and CD15+ cells revealed that neuronal survival and the likelihood of neurite outgrowth respond inversely to different culture media. Serially diluted concentrations of puromycin decreased cultured adult RGCs in a dose-dependent manner, demonstrating the potential usefulness of these adult neuronal cultures in screening assays. This novel culture system can be used to model in vivo neuronal behaviors. Studies can now be expanded in conjunction with other methodologies to study the neurobiology of function, aging, and diseases.
APA, Harvard, Vancouver, ISO, and other styles
4

Quail, Daniela F., Tamara J. Maciel, Kem Rogers, and Lynne M. Postovit. "A Unique 3D In Vitro Cellular Invasion Assay." Journal of Biomolecular Screening 17, no. 8 (June 15, 2012): 1088–95. http://dx.doi.org/10.1177/1087057112449863.

Full text
Abstract:
Three-dimensional (3D) cell culture techniques using a bioreactor have been used to co-culture various breast cancer cell lines. Comparisons between 3D co-cultures containing different proportions of breast cancer cell lines have been made with respect to cluster size, cell surface marker distribution, and Ki67 expression. Furthermore, an observed difference in invasion through collagen between co-cultures has been briefly reported. However, these assays have not yet been developed into a quantifiable methodology to assess the effects of drugs and/or microenvironments on cellular invasion. From a cancer perspective, two important aspects of cellular invasion that are often left out of in vitro assays are considerations about the 3D structural heterogeneity of the primary tumor and the ability of cells to migrate in all directions. Accordingly, we have taken advantage of the methodology previously described for 3D cell culture techniques and have developed a 3D invasion assay using cell clusters that can be used to assess the effects of different drugs and treatment conditions on cancer cell invasion. We also describe a novel whole-mount technique that permits fluorescence-based immunolocalization of proteins through the entire tumorsphere, without the need for sectioning. Our assay provides a simple, inexpensive, and physiologically relevant context to study cellular invasion in vitro, in a way that recapitulates an in vivo milieu.
APA, Harvard, Vancouver, ISO, and other styles
5

Olofsson, Karl, Valentina Carannante, Madoka Takai, Björn Önfelt, and Martin Wiklund. "Ultrasound-Based Scaffold-Free Core-Shell Multicellular Tumor Spheroid Formation." Micromachines 12, no. 3 (March 20, 2021): 329. http://dx.doi.org/10.3390/mi12030329.

Full text
Abstract:
In cancer research and drug screening, multicellular tumor spheroids (MCTSs) are a popular model to bridge the gap between in vitro and in vivo. However, the current techniques to culture mixed co-culture MCTSs do not mimic the structural architecture and cellular spatial distribution in solid tumors. In this study we present an acoustic trapping-based core-shell MCTSs culture method using sequential seeding of the core and shell cells into microwells coated with a protein repellent coating. Scaffold-free core-shell ovarian cancer OVCAR-8 cell line MCTSs were cultured, stained, cleared and confocally imaged on-chip. Image analysis techniques were used to quantify the shell thickness (23.2 ± 1.8 µm) and shell coverage percentage (91.2 ± 2.8%). We also show that the shell thickness was evenly distributed over the MCTS cores with the exception of being slightly thinner close to the microwell bottom. This scaffold-free core-shell MCTSs formation technique and the analysis tools presented herein could be used as an internal migration assay within the MCTS or to form core-shell MCTS co-cultures to study therapy response or the interaction between tumor and stromal cells.
APA, Harvard, Vancouver, ISO, and other styles
6

Jadhav, Rajesh Khanduji. "IN VITRO SCREENING OF CELL WALL DEGRADING ENZYME PRODUCTIVITY FROM FUNGAL CULTURE FILTRATES ON DEPROTEINISED PLANT FLUID BY CUP PLATE ASSAY." Fungal Territory 1, no. 2 (November 19, 2018): 5–9. http://dx.doi.org/10.36547/ft.2018.1.2.5-9.

Full text
Abstract:
The present study purpose is to evaluate the potentiality of the Deproteinised juice made from selected plants to induce the enzyme productivity by growing the fungi on it. It is because, in earlier findings, the DPJ was found potential in enhancing fungi and the plant growth when used as the medium. The internal factors are responsible in DPJ which induces plants and fungi growth. During the process of Green Crop Fractionation (GCF), the deproteinised (DPJ) obtained from tissues of cabbage, beet, lucerne (Alfalfa), carrot and Anathum (Dill) forages left after leaf protein extraction employed as a medium for the cultivation of mycelial biomass of Penicillium and Aspergillus fungi. The mycelial growth in vitro was compared with the glucose nitrate medium. The culture filtrates were used to screen different secreted hydrolytic or cell wall degrading enzymes.. The agar ‘cup‐plate’ diffusion technique has been applied to the quantitative determination of enzyme activity, principally to amylase, cellulase and protease. With all enzymes so far examined, the relationship between diameter of zone over a wide range secreted quantitatively was examined. All fungi grew well on DPJ in comparison to their growth on glucose nitrate (GN) medium. Comparatively with GN medium, lucerne DPJ was found having more mycelial cellular proliferation. When the fungi grown on different concentrations of substrates, enriched with carboxymethyl cellulose, casein and starch in deproteinised leaf extracts and GN medium, it was found that there was the enhancement in the mycelial dry weight grown on DPJ as compared with glucose nitrate medium. Penicillium showed more yield of enzyme activities especially of cellulases and amylases as compared to protease by cup plate method. There was enhancement of mycelial biomass when the concentrations of substrates increased from 1% to 2%, while there was no change in the activity of all enzymes by increasing the concentrations of substrates. Activities of the enzymes in vitro can be indicative of the pattern of organogenesis in callus cultures in further studies by fungal culture filtrates cultured on deproteinised fluid medium.
APA, Harvard, Vancouver, ISO, and other styles
7

Kitano, Otome, and Kohji Nakazawa. "Neuronal Differentiation of NT2 Cells in Monolayer and Spheroid Cultures." MATEC Web of Conferences 333 (2021): 07008. http://dx.doi.org/10.1051/matecconf/202133307008.

Full text
Abstract:
Metabolism and differentiation of cultured cells are influenced by changes in cellular morphology. In this study, we investigated the differences in cell proliferation and neuronal differentiation of NT2 cells in monolayer (2D) and spheroid (3D) cultures. In the monolayer culture, the cells adhered and extended on a tissue culture plate. For the spheroid culture, we fabricated a microwell chip comprising 195 circular microwells (600 ìm in diameter) on a cutture plate, and the surface was modified with polyethylene glycol to promote spheroid formation. The cells were aggregated in each microwell and formed spheroids within 24 hours of culture, and the spheroid morphology was maintained thoughout the culture period. Although the cell proliferation ability in monolayer culture was higher than that in spheroid culture, the neuronal differentiation ability of NT2 spheroid culture was higher than that in monolayer culture. Furthermore, the neuronal differentiation of NT2 spheroids was dramatically enhanced by retinoic acid treatment. These results indicate that NT2 cell properties are influenced by differences in cell morphologies, and that spheroid culture is a promising technique to induce neuronal differentiation.
APA, Harvard, Vancouver, ISO, and other styles
8

Kitano, Otome, and Kohji Nakazawa. "Neuronal Differentiation of NT2 Cells in Monolayer and Spheroid Cultures." MATEC Web of Conferences 333 (2021): 07008. http://dx.doi.org/10.1051/matecconf/202133307008.

Full text
Abstract:
Metabolism and differentiation of cultured cells are influenced by changes in cellular morphology. In this study, we investigated the differences in cell proliferation and neuronal differentiation of NT2 cells in monolayer (2D) and spheroid (3D) cultures. In the monolayer culture, the cells adhered and extended on a tissue culture plate. For the spheroid culture, we fabricated a microwell chip comprising 195 circular microwells (600 ìm in diameter) on a cutture plate, and the surface was modified with polyethylene glycol to promote spheroid formation. The cells were aggregated in each microwell and formed spheroids within 24 hours of culture, and the spheroid morphology was maintained thoughout the culture period. Although the cell proliferation ability in monolayer culture was higher than that in spheroid culture, the neuronal differentiation ability of NT2 spheroid culture was higher than that in monolayer culture. Furthermore, the neuronal differentiation of NT2 spheroids was dramatically enhanced by retinoic acid treatment. These results indicate that NT2 cell properties are influenced by differences in cell morphologies, and that spheroid culture is a promising technique to induce neuronal differentiation.
APA, Harvard, Vancouver, ISO, and other styles
9

Tan, Li Li, Liang Ren, Yuan Yuan Cao, Xiao Lin Chen, and Xin Yun Tang. "Bacterial Cellulose Synthesis in Kombucha by Gluconacetobacter sp and Saccharomyces sp." Advanced Materials Research 554-556 (July 2012): 1000–1003. http://dx.doi.org/10.4028/www.scientific.net/amr.554-556.1000.

Full text
Abstract:
Strain Gluconacetobacter hansenii CGMCC1671 and Saccharomyces cerevisiae CGMCC1670 were applied to make traditional Kombucha with pure cultures to search for the optimum parameters of major factors affecting the yields and productivities of Bacterial cellulose (BC) in the beverage. Three culture factors were examined. The yields and productivities of BC and sugar consumed were measured after cultured statically for 22 days. After single factor test factors affecting the yields and productivities of BC have been optimized by response surface methodology (RSM). The quadratic polynomial regression equation reflecting BC yield and affecting factors was build up with Box-Behnken design principle. The optimal values of 10.37% inoculum, initial pH 4.96 and medium volume 77.13 mL in 250 mL flask were obtained with theoretical BC yield 300.093mg/g. BC yield of 279.579 mg/g was obtained with 6.84% deviation by validation test with the optimal parameters. The co-culture of pure strains of traditional Kombucha technique can be used to provide both high quality and high yield of BC in addition to producing high quality Kombucha beverage.
APA, Harvard, Vancouver, ISO, and other styles
10

Gordillo-Fuenzalida, Felipe, Alex Echeverria-Vega, Sara Cuadros-Orellana, Claudia Faundez, Thilo Kähne, and Rodrigo Morales-Vera. "Cellulases Production by a Trichoderma sp. Using Food Manufacturing Wastes." Applied Sciences 9, no. 20 (October 18, 2019): 4419. http://dx.doi.org/10.3390/app9204419.

Full text
Abstract:
The cost of cellulase enzymes is a main contributor to the operational cost of a biorefinery producing ethanol from lignocellulosic material. Therefore, onsite production of enzymes using low-value substrates might be an option to make a bio-based facility more economical, while improving environmental sustainability. Food manufacturing wastes (FMWs), such as olive mill solids, tomato pomace, and grape pomace, are some of the main wastes produced by the food industry in Chile. FMWs are mostly composed of lignocellulosic material, which is primarily made of cellulose. A fungal strain obtained from olive stones was identified as a Trichoderma sp. and characterized by molecular and morphological techniques. This strain was able to grow on three FMWs in both liquid and solid cultures. In liquid cultures, cellulase and β-glucosidase activities from the culture supernatants were quantified. Identification of extracellular proteins using mass spectrometry revealed the presence of endoglucanases, exoglucanases, and β-glucosidases. Cellulase production from agroindustrial residues could be an excellent opportunity to utilize FMWs as well as decrease enzyme production costs in biorefinery processes.
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "Cellules – Culture – Technique"

1

Elluard, Marie-Paule. "Etude d'un bioréacteur à membranes pour la culture de cellules animales." Toulouse, INPT, 1990. http://www.theses.fr/1990INPT014G.

Full text
Abstract:
La culture de cellules animales en reacteur est difficile car ces cellules sont complexes, fragiles et sensibles a l'environnement physico-chimique. Il en resulte des couts de production eleves et aucun des procedes aujourd'hui proposes n'est satisfaisant a l'echelle industrielle. L'objectif de ce travail est de montrer qu'un bioreacteur a membranes minerales ou organiques, disposant d'une possibilite de limitation des phenomenes de colmatage par inversion du sens de circulation, assure un renouvellement du milieu de culture dans l'environnement immediat des cellules suffisant pour apporter les nutrients requis et pour eliminer les metabolites toxiques. La premiere partie fait l'etat de l'art concernant les bioreacteurs utilises dans l'industrie et ceux etudies a l'echelle du laboratoire. Dans les deuxieme et troisieme parties, le bioreacteur est decrit, des membranes sont selectionnees, l'intensite et la repartition des transferts de solvant sont etudiees. L'existence d'une configuration optimale pour l'homogeneite dans le reacteur est mise en evidence. Puis, une etude experimentale montre l'influence des parametres de fonctionnement sur le transfert de solvant et de solutes. Des lois de colmatage traduisant l'existence de phenomenes d'adsorption et de depot de matiere sont utilisees pour interpreter les resultats experimentaux obtenus. Une modelisation est proposee qui prevoit l'evolution du flux de transfert dans le bioreacteur. Pour conclure, un pilote de culture est concu et realise
APA, Harvard, Vancouver, ISO, and other styles
2

Claudon, Christine. "Mise en culture et caractérisation de cellules épithéliales mammaires bovines en sécrétion : optimisation des conditions de culture." Vandoeuvre-les-Nancy, INPL, 1992. http://www.theses.fr/1992INPL134N.

Full text
Abstract:
Parmi les systèmes de culture cellulaire utilisés dans l'étude des régulations de lactogènes, le plus employé est la culture sur collagène flottant de cellules obtenues par dissociation de tissu mammaire d'animaux mi-gestants. Peu d'auteurs parviennent à maintenir des lactocytes secrétant in vitro. Le but de ce travail est la construction d'un modèle permettant 1) le maintien de lactocytes bovins différenciés 2) la recherche de conditions de culture nécessaires a un métabolisme proche de celui observe in vivo 3) l'étude des secrétions protéiques. Diverses méthodes d'isolement des cellules ont été comparées et un protocole adapte au tissu bovin lactant a été élaboré. La suspension de massifs cellulaires obtenue est ensemencée sur gel de collagène fixe ou flottant. L'observation en microscopie optique et le marquage immunofluorescent des cytokeratines montrent le caractère épithélial et sécréteur des cellules ensemencées. En culture les cellules s'organisent en pavage polygonal caractéristique des épithélial. L'analyse des milieux de culture (dosages elisa) montrent que la sécrétion d'alpha s1-caséine et beta-lactoglobuline est maintenue une dizaine de jours, à un niveau plus élevé sur collagène flottant. La technique des chambres de culture a fond poreux a été adaptée a la culture de lactocytes bovins et son utilisation améliore la différenciation morphologique et fonctionnelle des cellules en culture
APA, Harvard, Vancouver, ISO, and other styles
3

Meng, Shiyun. "Préparation d'un substrat biodégradable et multifonctionnel et modulation électrique des fonctions cellulaires des osteoblasts = : Preparation of multifunctional biodegradable substrate and electrical modulation of osteoblast cellular functions." Doctoral thesis, Université Laval, 2010. http://hdl.handle.net/20.500.11794/21934.

Full text
Abstract:
L'activité cellulaire répond à la stimulation électrique (SE). Le but de cette thèse était le développement d'un composite multifonctionnel et l'étude de la réponse des ostéoblastes à la SE transmise par un tel composite. Les objectifs spécifiques étaient les suivants: a) synthèse des particules de haute conductivité en polypyrroles (PPy) avec des rendements élevés en contrôlant la taille et la régularité moléculaire; b) bioactivation des particules PPy par dopage à l'héparine (HE); c) préparation de composites multifonctionnels électriquement stables et présentant une haute affinité biologique; d) étude de la prolifération et de la minéralisation des ostéoblastes sur un échafaudage conducteur sous SE. Le chapitre I résume les phénomènes bioélectriques chez l'humain à différents niveaux, les mécanismes possibles de l'action de l'électricité sur les cellules, et l'étude de la SE en génie tissulaire osseux. Ce chapitre propose également une revue critique des différentes techniques et des différentes méthodologies de SE utilisées pour des études environnementales, scientifiques et pour les soins cliniques. Les hypothèses et les objectifs de la thèse sont présentés. Le chapitre II décrit la synthèse des nanoparticules en PPy par polymérisation en emulsion en utilisant le réactif de Fenton comme oxydant. Ces nanoparticules présentent une morphologie polygonale creuse, avec une épaisseur approximative de 50 nm et un diamètre de 400-500 nm. Les caractéristiques cristallines de ces nanoparticules ont été démontrées. Un mécanisme plausible de synthèse est proposé. Le chapitre III rapporte la bioactivation des particules en PPy en utilisant F héparine (HE) comme dopant, la préparation d'une membrane conductrice biodégradable, et la culture de fibroblastes sur ces membranes. Le dopage avec HE améliore la stabilité électrique de la membrane conductrice et augmente l'adhésion et la prolifération de cellules. Le chapitre IV démontre que la SE induite par la membrane conductrice peut moduler l'activité des ostéoblastes et accélérer la formation osseuse. Un champ électrique optimal de 200 mV/mm avec une durée de stimulation entre 2 et 8 h a favorisé la prolifération des ostéoblastes et augmenté l'expression et la production de ses marqueurs spécifiques de maturation (ALP) et de minéralisation (CO). Le chapitre V présente une discussion générale, le sommaire des conclusions et les perspectives de recherche pour le futur. L'implication de ces travaux en génie tissulaire et en santé humaine est également abordée.
APA, Harvard, Vancouver, ISO, and other styles
4

Cornet, François. "Effets des conditions de culture sur la différenciation des cellules de moelle osseuse." Littoral, 2002. http://www.theses.fr/2002DUNK0081.

Full text
Abstract:
Dans ce travail, les effets des sérums (FCS et Ultroser®) et ceux de la dexaméthasone sur la différenciation des cellules de moelle osseuse de lapin ont été analysés. Une culture en FCS en présence de dexaméthasone apparaît comme la plus favorable pour promouvoir la différenciation des cellules de moelle osseuse. Le traitement à la dexaméthasone favorise l'expression des marqueurs ostéoblastiques. Le choix du sérum n'est pas sans conséquences puisque seule une culture en Ultroser® permet l'expression de la sialoprotéine osseuse et de l'ostéonectine laquelle nécessite la présence de dexaméthasone. L'analyse de la composition de la matrice extracellulaire par l'électrophorèse bidimensionnelle montre des compositions protéiques différentes en fonction des sérums et des modifications importantes induites par la dexaméthasone. Quatre spots néosynthétisés en FCS et dexaméthasone pourraient correspondre à la Gla-protéine matricielle. Enfin, ces profils ne correspondent pas à ceux obtenus à partir d'os déminéralisé. Par "differential display", plusieurs produits d'amplification différentielle ont été observés à partir d'ARNm extraits de cellules de moelle osseuse cultivées en FCS et traitées à la dexaméthasone. L'un d'eux correspond au gène humain de la moésine. La quantification du niveau d'expression de la moésine par PCR semi-quantitative après normalisation par rapport au gène de référence 36B4 montre une réduction significative de son niveau d'expression allant de 4 à 6 fois. Le rôle de cette protéine dans l'organisation du cytosquelette suggère que la dexaméthasone module les capacités d'adhésion et de mobilité des cellules de moelle osseuse.
APA, Harvard, Vancouver, ISO, and other styles
5

Frayssinet, Patrick. "Applications biologiques de l'hydroxyatatite de calcium." Toulouse, INPT, 1990. http://www.theses.fr/1990INPT017G.

Full text
Abstract:
Ce mémoire concerne l'étude des possibilités d'utilisation de l'hydroxyapatite de calcium dans le secteur de la biologie et des biomatériaux. Le premier chapitre fait appel à des notions connues et concerne la structure tissulaire, cellulaire et moléculaire du tissu osseux. Ces notions ont été complétées par des travaux personnels sur la constitution du tissu osseux. Le second chapitre traite de l'évolution de la structure du tissu osseux en réponse à l'implantation d'un matériau étranger. Le troisième chapitre a trait aux mécanismes de biominéralisation se déroulant au voisinage des implants constitués d'hydroxyapatite. Le quatrième chapitre est consacré à la constituion et à l'étude de modèles biologiques permettant une vision parcellaire d'évènements complexes. Le cinquième chapitre concerne le développement d'un modèle biologique basé sur des cultures de cellules animales. Le chapitre six développe les techniques d'immobilisation de cellules animales sur un solide et les perspectives qu'elles ouvrent pour l'utilisation de l'hydroxyapatite en biologie. Dans le chapitre sept, les applications actuelles de l'hydroxyapatite de calcium en chirurgie orthopédiques sont évaluées et des nouvelles applications consacrées aux greffes de cellules sont développées. En conclusion, il semble que ce matériau soit aussi bien adapté à un usage en biologie humaine que pour des techniques de laboratoire. Les possibilités d'extension de son application paraissent très importantes.
APA, Harvard, Vancouver, ISO, and other styles
6

St-Pierre, Philippe. "Mise au point d'une technique d'isolation et de mise en culture de cellules endothéliales à partir du muscle strié." [S.l. : s.n.], 2005.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
7

St-Pierre, Philippe. "Mise au point d'une technique d'isolation et de mise en culture de cellules endothéliales à partir du muscle strié." Mémoire, Université de Sherbrooke, 2005. http://savoirs.usherbrooke.ca/handle/11143/3819.

Full text
Abstract:
En plus d'être un important utilisateur de glucose, le muscle strié, par ses lits vasculaires, a des impacts importants sur les paramètres hémodynamiques. La cellule endothéliale de ces lits vasculaires est impliquée dans plusieurs aspects de la régulation du débit sanguin. Des études in vivo dans différents modèles de résistance à l'insuline ont notamment montré que la cellule endothéliale semblait jouer un rôle important dans les changements de la perméabilité capillaire du muscle strié. Pour mieux comprendre leur rôle, l'étude directe des cellules endothéliales serait d'un atout majeur. Ainsi, nous avons développé une technique de séparation des cellules endothéliales à partir du muscle strié de rat utilisant une méthode d'immunomarquage magnétique. Pour ce faire, nous avons effectué différents essais afin de déterminer les conditions optimales. La première étape consistait à mettre au point la technique de culture de cellules endothéliales à partir de la lignée cellulaire HUVEC."--résumé abrégé par UMI.
APA, Harvard, Vancouver, ISO, and other styles
8

Auzanneau, Céline. "Identification et caractérisation des transports chlorure dans la cellule de Sertoli en culture primaire chez le rat." Poitiers, 2002. http://www.theses.fr/2002POIT2334.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Chorvatova, Alzbeta. "Propriétés électrophysiologiques des cellules isolées, en culture, de la zone fasciculée de la glande surrénale bovine : action de l'angiotensine II." Lyon 1, 1995. http://www.theses.fr/1995LYO10243.

Full text
Abstract:
L'angiotensine ii (ang ii) est l'hormone principale du systeme renine-angiotensine, provoquant entre autre la secretion d'hormones steroides. Les etudes biochimiques ont montre que l'ang ii induit la secretion de glucocorticoides (cortisol, corticosterone) synthetises dans la zone fasciculee du cortex surrenal bovin. Ce travail concerne les effets de l'ang ii sur les proprietes electrophysiologiques des cellules fasciculees, isolees du cortex surrenal de buf, en culture primaire. La technique du patch clamp en configuration de cellule entiere a ete utilisee soit en membrane rompue, soit en membrane perforee a l'aide d'un antibiotique: l'amphothericine b. Notre etude met en evidence la presence d'un courant transitoire sortant potassique de type i#a et de courants entrants de nature calcique. Une reponse biphasique est induite par l'ang ii (100 nm) sur le pic de courant transitoire sortant, qui augmente puis diminue. Un effet biphasique de l'ang ii est egalement decrit sur le potentiel de membrane (qui varie autour de -40 mv), ou une hyperpolarisation rapide est suivie d'une depolarisation plus lente. Alors que la phase de depolarisation pourrait impliquer plusieurs conductances, nous montrons que l'hyperpolarisation est due a une augmentation transitoire d'une conductance potassique, dependant du calcium libre intracellulaire. Tous ces effets sont lies a l'activation des recepteurs a l'ang ii de type at#1
APA, Harvard, Vancouver, ISO, and other styles
10

Lefrancq, Elisabeth. "Mise au point d'une technique immunoenzymatique ELISA de dosage de l'alpha 1-antitrypsine : applications à divers liquides biologiques." Paris 5, 1993. http://www.theses.fr/1993PA05P185.

Full text
APA, Harvard, Vancouver, ISO, and other styles
More sources

Books on the topic "Cellules – Culture – Technique"

1

Morgan, Sara J. Animal cell culture. London: BIOS Scientific in association with the Biochemical Society, 1993.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
2

1935-, Ikada Yoshito, and Enomoto Shōji 1936-, eds. Tissue engineering for therapeutic use 2: Proceedings of the Second International Symposium of Tissue Engineering for Therapeutic Use, Tokyo, 30-31 October 1997. Amsterdam: Elsevier, 1998.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
3

1935-, Ikada Yoshito, and Okano Teruo, eds. Tissue engineering for therapeutic use 3: Proceedings of the Third International Symposium of Tissue Engineering for Therapeutic Use, Tokyo, 4-5 September 1998. Amsterdam: Elsevier, 1999.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
4

1935-, Ikada Yoshito, and Yamaoka Yoshio, eds. Tissue engineering for therapeutic use 1: Proceedings of the First International Symposium of Tissue Engineering for Therapeutic Use, Kyoto, Japan, 2 March 1997. Amsterdam: Elsevier, 1998.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
5

International Symposium of Tissue Engineering for Therapeutic Use (4th 1999 Kyoto, Japan). Tissue engineering for therapeutic use 4: Proceedings of the Fourth International Symposium on Tissue Engineering for Therapeutic Use, Kyoto, 23-24th September 1999. Edited by Ikada Yoshito 1935- and Shimizu Yoshihiko. Amsterdam: Elsevier, 2000.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
6

Ryan, una s. endothelial cells: Vol. 1. Boca Raton, FL: CRC Press, 1995.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
7

Georgia, Barlovatz-Meimon, and Adolphe Monique, eds. Culture de cellules animales. Paris: Inserm, 2003.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
8

(Editor), Peter J. Hollenbeck, and James R. Bamburg (Editor), eds. Neurons: Methods and Applications for the Cell Biologist, Volume 71 (Methods in Cell Biology). Academic Press, 2003.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
9

(Editor), Peter J. Hollenbeck, and James R. Bamburg (Editor), eds. Neurons: Methods and Applications for the Cell Biologist, Volume 71 (Methods in Cell Biology). Academic Press, 2003.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
10

James, Hollenbeck Peter, Bamburg James R, and American Society for Cell Biology., eds. Neurons: Methods and applications for the cell biologist. San Diego: Academic Press, 2003.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
More sources

Book chapters on the topic "Cellules – Culture – Technique"

1

Zucco, Flavia, Isabella De Angelis, and Annalaura Stammati. "Cellular Models for In Vitro Toxicity Testing." In Animal Cell Culture Techniques, 395–422. Berlin, Heidelberg: Springer Berlin Heidelberg, 1998. http://dx.doi.org/10.1007/978-3-642-80412-0_21.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Medina-Gomez, F. "Impact of Tissue Culture Techniques on the Development of Agribusiness in Mexico." In Progress in Plant Cellular and Molecular Biology, 795–800. Dordrecht: Springer Netherlands, 1990. http://dx.doi.org/10.1007/978-94-009-2103-0_119.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Morris, Gareth, Mark Browne, Kirsti Murahidy, and Mike Jacka. "Christchurch Town Hall Complex: Post-Earthquake Ground Improvement, Structural Repair, and Seismic Retrofit." In Case Studies on Conservation and Seismic Strengthening/Retrofitting of Existing Structures, 145–72. Zurich, Switzerland: International Association for Bridge and Structural Engineering (IABSE), 2020. http://dx.doi.org/10.2749/cs002.145.

Full text
Abstract:
<p>The Christchurch Town Hall (CTH) complex contains six reinforced concrete buildings constructed circa 1970 in Christchurch, New Zealand (NZ). The complex is used for performing arts and entertainment, with an Auditorium that is internationally recognized for its acoustics. It is listed as a Grade-1 heritage building due to its cultural and historical significance. Unfortunately, the CTH foundation system was not originally designed to accommodate liquefaction-induced differential settlement and lateral spreading effects, as highlighted by the 2010–2011 Canterbury earthquake sequence. Although the most extreme ground motions exceeded the NZS 1170.5 code-defined 1/2500 year earthquake loads, the CTH structures performed remarkably well for a design that pre-dated modern seismic codes. Most of the observed structural damage was a result of the differential ground deformations, rather than in response to inertial forces. The post-earthquake observations and signs of distress are presented herein. The primary focus of this paper is to describe two major features of the seismic retrofit project (initiated in 2013) which were required to upgrade the CTH complex to meet 100% of current NZS 1170.5 seismic loadings. Firstly, the upgrade required extensive ground improvement and a new reinforce concrete mat slab to mitigate the impacts future ground deformations. Soil stabilization was provided by a cellular arrangement of jet-grout columns, a relatively new technique to NZ at the time. The new mat slab (typically 600-900 mm) was constructed over the stabilized soils. Secondly, upgrading the superstructure had many constraints that were overcome via a performance-based design approach, using non-linear time-history analysis. Recognizing the heritage significance, the superstructure “resurrection” as a modern building was hidden within the original skin minimized disruption of heritage fabric. Retrofit solutions were targeted, which also minimized the overall works. The 2015–2019 construction phase is briefly discussed within, including jet-grout procedures and sequencing considerations.</p>
APA, Harvard, Vancouver, ISO, and other styles
4

Payment, Pierre, and Michel Trudel. "CULTURE DE CELLULES EN FEUILLETS." In Manuel de techniques virologiques, 5–20. Presses de l'Université du Québec, 1989. http://dx.doi.org/10.2307/j.ctv18pgnrq.5.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

"Animal Cell Culture Techniques." In An Introduction to Metabolic and Cellular Engineering, 335–41. WORLD SCIENTIFIC, 2011. http://dx.doi.org/10.1142/9789814365734_0008.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Yannelli, John R., Martin R. Jadus, Suzanne Beckner, Leocadio V. Lacerna, and Robert K. Oldham. "Clinical/Technical Challenges in Adoptive Cellular Immunotherapy: In Vitro Culture Techniques." In Adoptive Cellular Immunotherapy of Cancer, 191–206. CRC Press, 2021. http://dx.doi.org/10.1201/9781003210115-12.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

"Techniques for Manipulating Cell Cultures and Studying Cellular Behaviour." In Manuals in Biomedical Research, 127–70. WORLD SCIENTIFIC, 2010. http://dx.doi.org/10.1142/9789812834782_0005.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Basiji, David. "Multispectral Imaging in Flow: A Technique for Advanced Cellular Studies." In Flow Cytometry for Biotechnology. Oxford University Press, 2005. http://dx.doi.org/10.1093/oso/9780195183146.003.0008.

Full text
Abstract:
Flow cytometry is one of the most sophisticated technologies for the study of cellular biology, with the unique ability to analyze cell populations numbering in the millions. In addition to their great speed, flow cytometers can be configured with 10 or more detectors, so each cell can be characterized by multiple parameters corresponding to light scattered or emitted as they pass through the system. These capabilities have led to the development of a wide variety of applications for flow cytometry in cell biology, hematology, immunology, oncology, cell culture, and other fields. Nevertheless, a wide range of applications are not suited to flow cytometric analysis because the resulting data fail to capture cell morphology or the spatial distribution of signals within a cell. In contrast to conventional flow cytometry, microscopy can elucidate cell morphology by a number of means, including absorbed light imaging (brightfield), scattered light imaging (darkfield), and fluorescence imaging. An image of a typical mammalian cell measuring approximately 10 mm in diameter will cover over 300 pixels, assuming a detector resolution of 0.5mm per pixel. Hence, even a single cell image represents orders of magnitude more data than is acquired by a flow cytometric analysis of that same cell and therefore may represent far more information about that cell. In theory, a set of brightfield, darkfield, and fluorescence images of each cell would provide all the information of flow cytometry plus morphological features, such as the nuclear to cytoplasmic ratio, membrane texture, the distribution of a fluorescent probe, and so forth. However, as a practical matter, it is difficult to acquire and compare more than a few images of a cell because of the need to change fluorescence filter combinations, reconfigure the microscope for brightfield and darkfield imaging, and register the various images to compensate for shifts and distortion from the different optical arrangements. As a result, flow cytometry and microscopy have remained complementary techniques. The goal of imaging cells in flow has been elusive, despite several approaches that have achieved various levels of success over the last several decades.
APA, Harvard, Vancouver, ISO, and other styles
9

Simpson, Michael L., and Timothy E. McKnight. "The Biology of Integration of Cells into Microscale and Nanoscale Systems." In Cellular Computing. Oxford University Press, 2004. http://dx.doi.org/10.1093/oso/9780195155396.003.0013.

Full text
Abstract:
In chapter 5 we focused on the informational interface between cells and synthetic components of systems. This interface is concerned with facilitating and manipulating information transport and processing between and within the synthetic and whole-cell components of these hybrid systems. However, there is also a structural interface between these components that is concerned with the physical placement, entrapment, and maintenance of the cells in a manner that enables the informational interface to operate. In this chapter we focus on this structural interface. Successful integration of whole-cell matrices into microscale and nanoscale elements requires a unique environment that fosters continued cell viability while promoting, or at least not blocking, the information transport and communication pathways described in earlier chapters. A century of cell culture has provided a wealth of insight and specific protocols to maintain the viability and (typically) proliferation of virtually every type of organism that can be propagated. More recently, the demands for more efficient bioreactors, more compatible biomedical implants, and the promise of engineered tissues has driven advances in surface-modification sciences, cellular immobilization, and scaffolding that provide structure and control over cell growth, in addition to their basic metabolic requirements. In turn, hybrid biological and electronic systems have emerged, capable of transducing the often highly sensitive and specific responses of cellular matrices for biosensing in environmental, medical, and industrial applications. The demands of these systems have driven advances in cellular immobilization and encapsulation techniques, enabling improved interaction of the biological matrix with its environment while providing nutrient and respiratory requirements for prolonged viability of the living matrices. Predominantly, such devices feature a single interface between the bulk biomatrix and transducer. However, advances in lithography, micromachining, and micro-/nanoscale synthesis provide broader opportunities for interfacing whole-cell matrices with synthetic elements. Advances in engineered, patterned, or directed cell growth are now providing spatial and temporal control over cellular integration within microscale and nanoscale systems. Perhaps the best defined integration of cellular matrices with electronically active substrates has been accomplished with neuronal patterning. Topographical and physicochemical patterning of surfaces promotes the attachment and directed growth of neurites over electrically active substrates that are used to both stimulate and observe excitable cellular activity.
APA, Harvard, Vancouver, ISO, and other styles
10

Conway, Gordon, Ousmane Badiane, and Katrin Glatzel. "The New Genetics." In Food for All in Africa, 157–87. Cornell University Press, 2019. http://dx.doi.org/10.7591/cornell/9781501743887.003.0008.

Full text
Abstract:
This chapter turns to genetic intensification, which consists of developing crop and livestock crosses that contain genes capable of producing improved yields on a sustainable basis. These crosses often show increased vigor, such that they tend to outperform both parents, although for reasons that are not fully clear. Today, hybrids and crosses are the basis for most improved crop and livestock breeds, including wheat, rice, maize, and dairy cattle. Nevertheless, as has been long recognized, conventional breeding techniques have practical limitations. The application of modern cellular and molecular biology is pursued through four practical techniques: marker-assisted selection, cell and tissue culture, recombinant DNA, and gene editing. The chapter examines the extent to which these interventions contribute to sustainable intensification: improving nutrition, increasing resilience to pests, diseases, and climate change, and improving nitrogen fixation.
APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "Cellules – Culture – Technique"

1

Mignone, Lindsay F., Shirley Masand, Jeffrey D. Zahn, and David I. Shreiber. "A Simple, Cost-Effective Method to Improve Cell Viability in Microniche Culture Systems." In ASME 2010 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2010. http://dx.doi.org/10.1115/sbc2010-19189.

Full text
Abstract:
Microfluidic networks are increasingly used to generate custom microenvironmental niches for cell culture and assays of cellular behavior. Perfusion systems are typically required to overcome diffusive limitations associated with culturing cells longer than a few hours when nutrient delivery, oxygen delivery and metabolic waste removal are required to maintain cell viability. In addition to the added complexity of experimental methods, perfusion systems can result in nonuniform nutrient delivery and subject cells to shear stresses, which may alter cell behavior and possibly cause cell death. In particular, when culturing cells within hydrogel scaffold-filled networks, as may be done in micro-tissue engineering, the need for perfusion culture also increases the likelihood of a destructive bubble entering the network. Moreover, analysis of micro-cultures frequently entails labelling with antibodies and/or fluorescent probes, which again requires controlled perfusion of the various reagents through the network. We have developed a simple technique to preserve cell viability and simplify labeling within microscale cultures without the need for perfusion. Instead of bonding a microfluidic network to glass, PDMS, or another impermeable substrate, the network is bonded to a semi-permeable microdialysis membrane, which allows free exchange of oxygen, proteins, nutrients, and waste between the microfluidic channels and culture media in static culture plates.
APA, Harvard, Vancouver, ISO, and other styles
2

Muravyeva, M. S., and Yu N. Zakharov. "Combination of structural and functional examination techniques of cellular cultures." In Frontiers in Optics. Washington, D.C.: OSA, 2014. http://dx.doi.org/10.1364/fio.2014.jw3a.16.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Chakraborty, Nilay, Wesley Parker, Kevin E. Elliott, Stuart T. Smith, Patrick J. Moyer, and Gloria Elliott. "Molecular Mobility in Trehalose Loaded Mammalian Cells: Time-Resolved Fluorescence Anisotropy Measurements." In ASME 2008 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2008. http://dx.doi.org/10.1115/sbc2008-193077.

Full text
Abstract:
Many preservation methods have utilized sugars such as trehalose as protectants against injury during cell preservation processing, especially during drying (1–5). As mammalian cells do not synthesize trehalose, research in the mammalian cell desiccation field has focused on the development of strategies to enable trehalose delivery into the intracellular milieu. Numerous techniques have been explored ranging from microinjection (2) to the creation or utilization of membrane pores (1,3). Fluid phase endocytosis has shown great promise as an effective strategy for non-invasively delivering water-soluble materials into the intracellular space (4, 5). In this technique trehalose is transported across the cell membrane in membrane-bound cellular compartments called endosomes. Cells incubated in cell culture medium containing trehalose have been shown to take up considerable amounts of trehalose by this technique (4, 5). How much of this trehalose actually become available for protection of biomolecules during the dehydration process has yet to be determined.
APA, Harvard, Vancouver, ISO, and other styles
4

Samuel, B. A., and M. A. Haque. "Mechanical Response of Single Biological Cells to Externally Applied Forces." In ASME 2007 International Mechanical Engineering Congress and Exposition. ASMEDC, 2007. http://dx.doi.org/10.1115/imece2007-43373.

Full text
Abstract:
We present an experimental technique capable of applying and measuring very small forces (nanoNewtons) on live biological cells. The technique is based on combing microfabricated molded elastomeric microneedles, and substrate deformation to actively apply loads on single cells. By using fluorescence and differential interference contrast imaging we can quantitatively study the response of biological cells to force exerted on it via the extra cellular matrix. Bovine Aortic Endothelial Cells were cultured on the micropillars and strained in-vitro to demonstrate the experimental technique.
APA, Harvard, Vancouver, ISO, and other styles
5

Li, Rongheng, Nilay Chakraborty, and Ben Q. Li. "Understanding Temperature Profiles Experienced by Biological Samples During a Hybrid Vitrification Technique." In ASME 2013 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/imece2013-63947.

Full text
Abstract:
From thermodynamic point of view, vitrification is considered as a superior preservation technique in comparison with the traditional slow-cooling cryopreservation techniques, due to formation of the glassy state in both intra and extracellular environment. While vitrification of biological samples are difficult to achieve, recently a hybrid technique involving partial desiccation of the cell samples prior to cryogenic exposure has been successfully employed to achieve vitrification. In this technique cells in monolayer attached to a substrate was suspended in a trehalose solution and then rapidly and uniformly desiccated to a low moisture content (<0.12 g of water per g of dry weight) using a spin-drying technique. The spin-dried samples were stored in liquid nitrogen (LN2) at a vitrified state (Fig. 1). It was shown that following re-warming to room temperature and re-hydration with a fully complemented cell culture medium, 51% of the spin-dried and vitrified cells survived and demonstrated normal growth characteristics. The current study further investigates the temperature profiles experienced by the cell samples during partial desiccation and cryogenic exposure to identify possible ways to improve this novel vitrification strategy. Physical Vapor Deposition technique was employed to develop glass substrate having thermocouples (2×2×2 μm) at four radial positions across the substrate to record the thermal history of the cell samples during the entire process. Efforts are undertaken to understand the uncertainties related temperature measurement spatially and with respect to time. These temperature characterization studies are important to optimize the newly developed hybrid vitrification technique for vitrification of cellular samples.
APA, Harvard, Vancouver, ISO, and other styles
6

Kim, Eun Jung, Cynthia Boehm, Aaron J. Fleischman, George F. Muschler, Yordan Kostov, and Shuvo Roy. "Modulating Human Connective Tissue Progenitor (CTP) Cell Behavior on Cellulose Acetate Scaffolds by Surface Microtextures." In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-175229.

Full text
Abstract:
It has been shown that precise microarchitecture and surface microtexture can enhance cell behavior such as cell attachment, proliferation, migration and differentiation in cell culture environment [1]. Even though Polydimethylsiloxane (PDMS) has been commonly used to fabricate the microtextured surfaces, PDMS is not biodegradable. Cellulose Acetate (CA), on the other hand, has very promising properties as scaffold material for tissue engineering applications. It is biodegradable, has low water solubility, and generates minimal foreign body reaction in vivo [2]. In this study, soft lithography techniques are used to fabricate CA and PDMS scaffolds with precise surface microtextures to compare growth characteristics of progenitor cells.
APA, Harvard, Vancouver, ISO, and other styles
7

Zavrel, Erik A., Michael L. Shuler, and Xiling Shen. "A Simple Aspect Ratio Dependent Method of Patterning Microwells for Selective Cell Attachment." In 2018 Design of Medical Devices Conference. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/dmd2018-6811.

Full text
Abstract:
3-D culture has been shown to provide cells with a more physiologically authentic environment than traditional 2-D (planar) culture [1, 2]. 3-D cues allow cells to exhibit more realistic functions and behaviors, e.g., adhesion, spreading, migration, metabolic activity, and differentiation. Knowledge of changes in cell morphology, mechanics, and mobility in response to geometrical cues and topological stimuli is important for understanding normal and pathological cell development [3]. Microfabrication provides unique in vitro approaches to recapitulating in vivo conditions due to the ability to precisely control the cellular microenvironment [4, 5]. Microwell arrays have emerged as robust alternatives to traditional 2D cell culture substrates as they are relatively simple and compatible with existing laboratory techniques and instrumentation [6, 7]. In particular, microwells have been adopted as a biomimetic approach to modeling the unique micro-architecture of the epithelial lining of the gastrointestinal (GI) tract [8–10]. The inner (lumen-facing) surface of the intestine has a convoluted topography consisting of finger-like projections (villi) with deep well-like invaginations (crypts) between them. The dimensions of villi and crypts are on the order of hundreds of microns (100–700 μm in height and 50–250 μm in diameter) [11]. While microwells have proven important in the development of physiologically realistic in vitro models of human intestine, existing methods of ensuring their surface is suitable for cell culture are lacking. Sometimes it is desirable to selectively seed cells within microwells and confine or restrict them to the microwells in which they are seeded. Existing methods of patterning microwells for cell attachment either lack selectivity, meaning cells can adhere and migrate anywhere on the microwell array, i.e., inside microwells or outside of them, or necessitate sophisticated techniques such as micro-contact printing, which requires precise alignment and control to selectively pattern the bottoms of microwells for cell attachment [12, 13].
APA, Harvard, Vancouver, ISO, and other styles
8

Higuita-Castro, Natalia, Cosmin Mihai, Derek J. Hansford, and Samir N. Ghadiali. "In-Vitro Model of the Microscale Alveolar Environment." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53648.

Full text
Abstract:
The present work describes the development of a novel micro-nanoscale system that more closely resembles the alveolar-capillary barrier in the lung by recapitulating different parameters of the cellular microenvironment, including fibrous geometry, fiber stiffness, chemistry, and cell-cell interactions. The system consists of a three-dimensional multilayered structure. Two microchannel chambers that resemble alveolar/airway space and the capillary lumen, interfaced with a porous mesh of polymeric nanofibers that act as the basal substrate for seeding lung epithelial and endothelial cell. The top and bottom chambers of the device were fabricated using soft lithography techniques, while the nanofiber mesh was obtained via electrospinning. Human alveolar epithelial cells (A549) and human umbilical vein endothelial cells (HUVEC) were successfully co-cultured using this system. Various cellular and molecular biology techniques are being employed to investigate injury patterns and overall cell responses under different circumstances which mimic various lung disorders such acute lung injury, pulmonary fibrosis and emphysema.
APA, Harvard, Vancouver, ISO, and other styles
9

Wang, Hai, and Wei Li. "Selective HIFU Foaming to Fabricate Porous Polymer for Tissue Engineering Scaffolds." In ASME 2006 International Manufacturing Science and Engineering Conference. ASMEDC, 2006. http://dx.doi.org/10.1115/msec2006-21043.

Full text
Abstract:
A novel technique is presented in this paper for the fabrication of tissue engineering scaffolds using the High Intensity Focused Ultrasound (HIFU). This acoustic method is a solvent-free, highly efficient and low cost process that has the potential in scaffold-based tissue engineering. HIFU fabrication technique is capable of creating hierarchically-structured porous polymeric materials, which have various topographical features at different length scales. This will in turn affect the cellular response and behavior of certain type of cells, such as the integration and growth of smooth muscle cells (SMCs). In this study, the effect of HIFU porous polymer fabrication was investigated. Scanning-mode HIFU insonation was performed in the HIFU polymer foaming experiments. The acoustic power and the scanning speed were chosen as the parameters and varied in different groups of experiments. The created microstructures were characterized using the scanning electron microscopy (SEM). The fabricated samples were used for cell culture studies with human aortic SMCs (Passage 4). It was found that the selective HIFU foaming process could be used to create hierarchical structures by choosing appropriate ultrasound parameters. The SMCs were viable on the HIFU-created porous PMMA specimens, and the topographical nature of a HIFU-created porous structure affected the cellular response of SMCs.
APA, Harvard, Vancouver, ISO, and other styles
10

Nain, Amrinder S., Eric Miller, Metin Sitti, Phil Campbell, and Cristina Amon. "Fabrication of Single and Multi-Layer Fibrous Biomaterial Scaffolds for Tissue Engineering." In ASME 2008 International Mechanical Engineering Congress and Exposition. ASMEDC, 2008. http://dx.doi.org/10.1115/imece2008-67964.

Full text
Abstract:
For regenerative medicine applications, we need to expand our understanding of the mechanisms by which nature assembles and functionalizes specialized complex tissues to form a complete organism. The first step towards this goal involves understanding the underlying complex mechanisms of highly organized behavior spanning not only diverse scientific fields, but also nano, micro and macro length-scales. For example, an engineered fibrous biomaterial scaffold possessing the hierarchal spatial properties of a native extracellular matrix (ECM) can serve as a building block upon which living cells are seeded for repair or regeneration. The hierarchical nature of ECM along with the inherent topological constraints of fiber diameter, fiber spacing, multi-layer configurations provide different pathways for living cells to adapt and conform to the surrounding environment. Our previously developed Spinneret based Tunable Engineered Parameters (STEP) technique to deposit biomaterial scaffolds in aligned configurations has been used for the first time to deposit single and multi-layer biological scaffolds of fibrinogen. Fibrinogen is a very well established tissue engineering scaffold material, as it improves cellular interactions and allows scaffold remodeling compared to synthetic polymers. Current state-of-the-art fiber deposition techniques lack the ability to fabricate scaffolds of desired fiber dimensions and orientations and in this study we present fabrication and aligned deposition of fibrinogen fiber arrays with diameters ranging from sub-200 nm to sub-microns and several millimeters in length. The fabricated scaffolds are then cultured with pluripotent mouse C2C12 cells for seven days and cells on the scaffolds are observed to elongate resembling myotube morphology along the fiber axis, spread along intersecting layers and fuse into bundles at the macroscale. Additionally, we demonstrate the ability to deposit poly (lactic-co-glycolic acid) (PLGA), Polystyrene (PS) biomaterial scaffolds of different diameters to investigate the effects of topological variations on cellular adhesion, proliferation and migration. Previous studies have indicated cells making right angle transitions upon encountering perpendicular double layer fibers and cellular motion is thwarted in the vicinity of diverging fibers. Current ongoing studies are aimed at determining the effects of fiber diameter and fiber spacing on mouse C2C12 cellular adhesion and migration, which are envisioned to aid in the design of future scaffolds for tissue engineering possessing appropriate material and geometrical properties.
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the extended bibliographies on the topic 'Cellules – Culture – Technique' for particular source types:
Journal articles Dissertations / Theses Books
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography