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Journal articles on the topic 'Cellulolytic activity'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Lategan, M. J., K. Korbel, and G. C. Hose. "Is cotton-strip tensile strength a surrogate for microbial activity in groundwater?" Marine and Freshwater Research 61, no. 3 (2010): 351. http://dx.doi.org/10.1071/mf09144.

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The cotton strip assay uses the loss of tensile strength of cotton strips as a measure of microbial cellulolytic activity. Its suitability for measuring general microbial activity in groundwater was tested by examining the relationship of tensile strength, abundance of cellulolytic organisms and general microbial activity on cotton strips deployed in bores. The hypothesis was that the strength of cotton strips would decline with increasing abundance and activity of cellulolytic organisms, and as cellulolysis makes resources available to other microbial groups, cotton strength loss should also

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Singh, Raj, Anju Rani, Permod Kumar, Gyanika Shukla, and Amit Kumar. "Cellulolytic Activity in Microorganisms." Bulletin of Pure & Applied Sciences- Botany 36b, no. 1 (2017): 28. http://dx.doi.org/10.5958/2320-3196.2017.00004.0.

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Dewiyanti, I., D. Darmawi, Z. A. Muchlisin, et al. "Cellulase enzyme activity of the bacteria isolated from mangrove ecosystem in Aceh Besar and Banda Aceh." IOP Conference Series: Earth and Environmental Science 951, no. 1 (2022): 012113. http://dx.doi.org/10.1088/1755-1315/951/1/012113.

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Abstract Cellulolytic bacteria is one of the beneficial bacteria that can found in mangrove ecosystem. The purposes of study were to analyse the cellulolytic index, and to analyse the cellulase activity of bacteria isolated from soil mangrove. Qualitatively, assessment of cellulase activity were carried out at the Microbiology laboratory of Fish Quarantine Station, Quality Control and Safety of Fishery Products (SKIPM) Aceh, while quantitatively was observed in microbiology laboratory, Biology Department, IPB. Assessment of qualitative cellulase activity is performed by growing the selected pu

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Nasution, A. F., E. Munir, D. Suryanto, and Y. Yurnaliza. "Potential of cellulolytic fungi isolated from the intestine of Asiatic rhinoceros beetle (Oryctes rhinoceros)." IOP Conference Series: Earth and Environmental Science 1115, no. 1 (2022): 012072. http://dx.doi.org/10.1088/1755-1315/1115/1/012072.

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Abstract Cellulose is a potential source of fermentable sugars and a promising cost-effective source for biofuel production. This study aimed to obtain potential cellulolytic fungi from the intestine of Asiatic rhinoceros beetle (Oryctes rhinoceros L.). The cellulolytic fungi were isolated using Potato Dextrose Agar (PDA) media using spread plate method. Qualitative and quantitative screening tests were carried out using 1% of CMC (w/v) as a sole carbon source. Twelve isolates were recovered with varying cellulolytic capabilities. The highest cellulolytic activity was obtained from KOr-15J wit

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Zadorozhnaya, Galina, and Katerina Andrusevych. "The Relationship Between Soil Heterogeneity and Cellulolytic Activity." Ekológia (Bratislava) 40, no. 1 (2021): 1–7. http://dx.doi.org/10.2478/eko-2021-0001.

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Abstract This article examines the relationship between soil heterogeneity and cellulolytic activity. The investigated substrate was a typical chernozem (black soil). Cellulolytic activity was studied by the application method across a regular grid of 7×15 points (21×45 m). The distance between the measurement points was 3 m. Soil heterogeneity determined by the measurement of soil penetration resistance using a hand penetrometer Eijkelkamp. Measurements of soil penetration resistance were fixed to a depth of 100 cm at intervals of 5 cm. Geostatistical analysis showed a high level of spatial d

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Simbolon, L. M., M. S. Ismet, P. Ismiati, A. F. Ilham, and E. S. Srimariana. "Cellulolytic activity at the low temperature of associative bacteria from a seagrass ecosystem." IOP Conference Series: Earth and Environmental Science 1137, no. 1 (2023): 012039. http://dx.doi.org/10.1088/1755-1315/1137/1/012039.

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Abstract Cellulase enzymes have been widely used in many industrial activities, such as the production of biofuels, biofertilizers, and bio-decomposers. The usage of cellulase enzymes on an industrial scale usually involves a relatively high temperature. Microorganisms in marine ecosystems are widely known to have the ability to produce cellulase enzymes in various environmental ranges. However, exploring marine cellulolytic bacteria at low temperatures still lacks information. This study aims to determine the cellulolytic activity of bacteria associated with seagrass ecosystems at low tempera

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Rosyidi, Muhammad Subhan Bahruddin, Muhammad Ali, Wayan Wariata, et al. "Isolation and Identification of Swan (Cygnus olor) Digestive Tract Cellulothic Bacteria to Support Fiber Degradation." Jurnal Penelitian Pendidikan IPA 10, SpecialIssue (2024): 572–78. http://dx.doi.org/10.29303/jppipa.v10ispecialissue.8516.

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Cellulolytic bacteria are bacteria that produce cellulase enzymes capable of degrading cellulose substrates. This study aimed to isolate and identify as well as measure the cellulase enzyme activity of cellulolytic bacteria isolated from the digestive tract of swans (Cygnus olor) in Mataram. The bacteria were isolated using de Man Rogosa Sharpe (MRS) selective media with the spread plate method. Then, the extracellular enzyme activity test was conducted by growing selected pure isolates on 1% Carboxy Methyl Cellulose (CMC 1%) media, followed by pouring 0.1% congo red solution and rinsing with

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8

Nasution, A. F., E. Munir, and D. Suryanto. "Isolation and screening of local cellulolytic fungi from the digestive tract larvae of Oryctes rhinoceros L., North Sumatra." IOP Conference Series: Earth and Environmental Science 912, no. 1 (2021): 012047. http://dx.doi.org/10.1088/1755-1315/912/1/012047.

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Abstract Cellulose, which is the main component of plant cell walls from higher plants, has been studied from different aspects. It is insoluble in a wide variety of solvents and is resistant to various chemicals treatments. Fungi are a group of cellulose-degrading microbes and plays major role in recycling of lignocellulosic material in nature. This study aimed to obtain cellulolytic fungi from the digestive tract of Oryctes rhinoceros L. larvae and to determine cellulolytic activity. Isolation and screening of cellulolytic fungi in the digestive tract of insects were carried out with specifi

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9

Grata, Katarzyna. "Determining Cellulolytic Activity of Microorganisms." Chemistry-Didactics-Ecology-Metrology 25, no. 1-2 (2020): 133–43. http://dx.doi.org/10.2478/cdem-2020-0010.

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Abstract Decomposition of cellulose to glucose requires complex cooperation of glycoside hydrolase enzymes. As a result of glycoside β-1,4 bonds hydrolysis, shorter chains of cellulose, oligodextrin, cellobiose and glucose are created. A number of bacteria and fungi demonstrate the capacity to degrade cellulose. Their activity can be assessed with the use of qualitative and quantitative methods. Qualitative methods with the use of e.g. Congo red, are used in screening studies, however, they do not provide information about the quantity of the produced enzyme. Spectrophotometric methods are mor

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Lee, Song F., Cecil W. Forsberg, and L. N. Gibbins. "Cellulolytic Activity of Clostridium acetobutylicum." Applied and Environmental Microbiology 50, no. 2 (1985): 220–28. http://dx.doi.org/10.1128/aem.50.2.220-228.1985.

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Ramesh, A., and V. K. Venugopalan. "Cellulolytic activity of luminous bacteria." MIRCEN Journal of Applied Microbiology and Biotechnology 4, no. 2 (1988): 227–30. http://dx.doi.org/10.1007/bf01301952.

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12

Yamada, Ryosuke, Tomohisa Hasunuma, and Akihiko Kondo. "Endowing non-cellulolytic microorganisms with cellulolytic activity aiming for consolidated bioprocessing." Biotechnology Advances 31, no. 6 (2013): 754–63. http://dx.doi.org/10.1016/j.biotechadv.2013.02.007.

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Yu, Nan-Nan, Wirinthip Ketya, and Gyungsoon Park. "Intracellular Nitric Oxide and cAMP Are Involved in Cellulolytic Enzyme Production in Neurospora crassa." International Journal of Molecular Sciences 24, no. 5 (2023): 4503. http://dx.doi.org/10.3390/ijms24054503.

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Although molecular regulation of cellulolytic enzyme production in filamentous fungi has been actively explored, the underlying signaling processes in fungal cells are still not clearly understood. In this study, the molecular signaling mechanism regulating cellulase production in Neurospora crassa was investigated. We found that the transcription and extracellular cellulolytic activity of four cellulolytic enzymes (cbh1, gh6-2, gh5-1, and gh3-4) increased in Avicel (microcrystalline cellulose) medium. Intracellular nitric oxide (NO) and reactive oxygen species (ROS) detected by fluorescent dy

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Chantarasiri, Aiya. "Diversity and Activity of Aquatic Cellulolytic Bacteria Isolated from Sedimentary Water in the Littoral Zone of Tonle Sap Lake, Cambodia." Water 13, no. 13 (2021): 1797. http://dx.doi.org/10.3390/w13131797.

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Tonle Sap Lake is the largest freshwater lake in Southeast Asia, and it is regarded as one of the most biodiverse freshwater ecosystems in the world. Studies concerning aquatic cellulolytic bacteria from Tonle Sap Lake remain scarce. Cellulolytic bacteria and their cellulases play a vital role in the biogeochemical cycles of lake environments, and their application in biotechnological industries is likewise an important component of their usage. This study aimed to assess the isolation, genetic identification, bioinformatic analyses, and activity characterization of aquatic cellulolytic bacter

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Akhir, J., S. W. Budi, E. N. Herliyana, and Surono. "Lignocellulolytic enzyme potential of dark septate endophyte (dse) from Pinus merkusii roots in Dramaga Bogor Indonesia." IOP Conference Series: Earth and Environmental Science 959, no. 1 (2022): 012031. http://dx.doi.org/10.1088/1755-1315/959/1/012031.

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Abstract The ability of Dark Septate Endophyte (DSE) derived from the roots of P. merkusii in decomposing organic matter is not much published. This study aims to determine the activity of lignolytic and cellulolytic enzymes, as well as the value of the cellulolytic index of Apls, isolates from P. merkusii roots taken from IPB Dramaga Bogor. Enzyme activity was tested by measuring the clear zone formed, while the cellulolytic index value was obtained by measuring the ratio of the clear zone formed around the colony. Data were analyzed descriptively. The results identified 10 isolates, 8 Apls i

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Golwalkar, Sucheta, and Farida Khatri. "Enrichment and Characterisation of Cellulose Degrading Bacteria." Defence Life Science Journal 8, no. 2 (2023): 116–24. http://dx.doi.org/10.14429/dlsj.8.18061.

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In the current research, a total of 10 bacterial isolates have been collected from municipal waste, vegetable waste, a dumping ground, a rice field, termite waste, cotton dyeing industry waste, forest waste, the soil under a coconut tree, dead decomposed plant waste, and sewage waste.Using soluble carboxy methyl cellulose degradation assays, the 10 bacterial strains have been isolated and examined for cellulolytic activity. In one percentage CMC agar medium, four strains, namely C9D, C9E, C9C, and C9B, efficiently digested cellulose, with a cellulolytic index of 35 mm, 25 mm, 17 mm, and 18 mm,

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Krzyśko-Łupicka, Teresa, Łukasz Kręcidło, and Magdalena Kręcidło. "The Comparison of Cellulolytic Activity of the Modified Soil Treated with Roundup." Chemistry-Didactics-Ecology-Metrology 21, no. 1-2 (2016): 133–39. http://dx.doi.org/10.1515/cdem-2016-0012.

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AbstractThe activity of soil microorganisms affects soil fertility and structure, what leads to satisfactory crop yields, thanks to changes in the decay of organic matter. Their activity may be impaired as a result of application by farmers both fertilizers and pesticides. The degradation of cellulosic biomass represents an important part of the carbon cycle within the biosphere. Cellulolitic microorganisms are responsible for this decomposition, as they produced the enzymes of the cellulase complex. The aim of the study was to compare the cellulolytic activity of soil with Roundup and modifie

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Fitriadi, Ren, Aninditia Sabdaningsih, Slamet Budi Prayitno, Petrus Hary Tjahja Soedibya, Sarjito Sarjito, and Subagiyo Subagiyo. "Activity of Cellulolytic Bacteria Isolated From The Digestive Tract Of Jerbung Shrimp (Feneus marguensis)." Journal of Aquaculture and Fish Health 14, no. 1 (2025): 65–78. https://doi.org/10.20473/jafh.v14i1.66897.

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Bacteria found in the digestive tract of shrimp mostly play a major role in nutrient absorption. One of the nutritional contents that is quite difficult for shrimp to absorb is fiber. Bacteria that can degrade fiber content are known as cellulolytic bacteria. This study aimed to determine the activity index of cellulolytic bacteria found in the digestive tract of shrimp. The research method includes sampling shrimp with three different categories based on average length and weight, divided into three categories such as large (22.2 g ± 87.3 cm), medium (17.1 g ± 36.7 cm), and small (13.4 g ± 18

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Purba, Nursatria, Ida Bagus Wayan Gunam, and I. Made Mahaputra Wijaya. "Produksi Enzim Selulase Kasar dari Isolat Bakteri B2S8 menggunakan Substrat Brangkasan Jagung dengan Perlakuan Konsentrasi Inokulum dan Komposisi Media yang berbeda." JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI 8, no. 2 (2020): 267. http://dx.doi.org/10.24843/jrma.2020.v08.i02.p11.

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The purpose of this study was determined the media and concentration of cellulolytic bacterial isolates to produce high cellulase enzyme activity. Production of crude cellulase enzyme in media and concentration of different bacterial isolate used a factorial Randomized Block Design (RBD) which consist of two factors. The first factor was the media production of different cellulase enzyme consisting of 3 levels, namely media 1, 2 and 3. The second factor was the concentration of bacterial isolate consisting of 5 levels namely 1, 2, 3, 4 and 5%. This study used a B2S8 cellulolytic bacterial isol

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Onyia, D. C., E. C. Onyeneke, T. Okunwaye, et al. "The Influence Of Metal Ions On Cellulolytic Activities Of Fungal Isolates From Palm Biomass." Nigerian Journal of Biotechnology 38, no. 2 (2022): 67–72. http://dx.doi.org/10.4314/njb.v38i2.7.

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Numerous metal ions and chemical compounds can influence the activity of some fungi. Some have inhibitory effects whereas others have enhancing effects. In this study, the effect of metallic chlorides; lead II chloride (PbCl2), potassium chloride (KCl), copper II chloride (CuCl2), calcium II chloride (CaCl2), manganese II chloride (MnCl2), iron III chloride (FeCl3) and mercury II chloride (HgCl2) were evaluated for their influence on the cellulolytic activity of seven (7) fungal isolates at a concentration of 0.005M at 30°C for four (4) days using 3, 5-dinitrosalicyclic acid (DNS) method. CaCl

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Vásquez, Ernestina, and Carlos Millones. "Isolation and Identification of Bacteria of Genus Bacillus from Composting Urban Solid Waste and Palm Forest in Northern Peru." Microorganisms 11, no. 3 (2023): 751. http://dx.doi.org/10.3390/microorganisms11030751.

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A technical challenge for composting in Peruvian cities with annual temperatures below 20 °C is that the degradation of municipal solid waste (MSW) is slow, so the identification of cold-adapted bacteria would be interesting for use as inoculants in places with these climatic conditions. This study isolated, identified, and evaluated bacterial strains with cellulolytic and amylolytic activities at low temperatures. Bacterial strains were isolated from the Chachapoyas Municipal Composting Plant and soil from the Ocol Palm Forest in northern Peru. The screening was carried out to evaluate the ex

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Jiotode, Vaishnavi A., Chandrakant T. Kumbhar, Ashok C. Jadhav, et al. "Studies on Cellulolytic Fungal Flora from Forests of Maharashtra, India." International Journal of Environment and Climate Change 14, no. 2 (2024): 993–1001. http://dx.doi.org/10.9734/ijecc/2024/v14i24012.

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The present investigation was conducted to isolate effective cellulolytic fungal flora from soil samples collected from various forests in Maharashtra (India). The study aimed to determine hydrolytic zones and relative enzyme activity for selecting most efficient fungal isolates. Further, the isolates were identified using morphological identification technique. Altogether 137 fungal isolates were obtained in the present study, collected from forests of Lonavala, Chandrapur, Mahabaleshwar, Gadchiroli and Bhandardara. The screening of these fungal isolates for cellulase production revealed that

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Uddin, Mohammad Mosleh, Suzana Afrin Lima, Tanim Jabid Hossain, Newton Kar, Yeasmin Zahan та Babamale AbdulKareem Olarewaju. "Evaluation of Cellulolytic Endo-1,4-β-D-Glucanase Activity in the Digestive Fluid of Adult Phytophagous Beetle Hoplasoma unicolor". Tropical Life Sciences Research 32, № 3 (2021): 53–68. http://dx.doi.org/10.21315/tlsr2021.32.3.4.

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Insects of the taxonomic order Coleoptera are recognised for considerable cellulolytic activity in their digestive fluid. The cellulolytic activity of the gut fluid in Hoplasoma unicolor, a member of Coleoptera, however, remains unexplored. In this study, we, for the first time, report the qualitative and quantitative analysis of cellulolytic activity in the digestive fluid of this insect. The cellulolytic endo-1,4-β-D-glucanase activity was confirmed in the supernatant of the insect’s digestive fluid by agar plate assay using carboxymethyl cellulose as the substrate. To determine the optimum

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Uddin, Mohammad Mosleh, Suzana Afrin Lima, Tanim Jabid Hossain, Newton Kar, Yeasmin Zahan та Babamale AbdulKareem Olarewaju. "Evaluation of Cellulolytic Endo-1,4-β-D-Glucanase Activity in the Digestive Fluid of Adult Phytophagous Beetle Hoplasoma unicolor". Tropical Life Sciences Research 32, № 3 (2021): 53–68. http://dx.doi.org/10.21315/tlsr2021.32.3.4.

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Insects of the taxonomic order Coleoptera are recognised for considerable cellulolytic activity in their digestive fluid. The cellulolytic activity of the gut fluid in Hoplasoma unicolor, a member of Coleoptera, however, remains unexplored. In this study, we, for the first time, report the qualitative and quantitative analysis of cellulolytic activity in the digestive fluid of this insect. The cellulolytic endo-1,4-β-D-glucanase activity was confirmed in the supernatant of the insect’s digestive fluid by agar plate assay using carboxymethyl cellulose as the substrate. To determine the optimum

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Farihah, Lailatul, Sumardi Sumardi, C. N. Ekowati, and Sutopo Hadi. "Cellulase Activity Bacillus sp. Which Was Grown on A Medium with Aluminum Salt and Magnetic Field Exposure." Jurnal Sumberdaya Alam dan Lingkungan 10, no. 1 (2023): 22–29. http://dx.doi.org/10.21776/ub.jsal.2023.010.01.3.

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Aluminum is the best metal cofactor enzyme. Exposure to the 0,2mT magnetic field on Aluminium salt is said to be able to increase cellulolytic activity. The purpose of the research is to know if the best aluminum salt can increase cellulolytic activity. This can be tested by Cellulolytic Index Test and Enzyme Activity Test. This experiment is descriptive research with three treatments; control, the addition of aluminum salt without an exposed magnetic field, and added aluminum salt exposed to a magnetic field. Salt aluminum used are AlCl3, Al2(PO4)3, and Al2(SO4)3. The concentration was 0.01%

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Murali, H. S., M. S. Mohan, K. S. Manja, and R. Sankaran. "Cellulolytic activity of four Fusarium spp." World Journal of Microbiology & Biotechnology 10, no. 4 (1994): 487. http://dx.doi.org/10.1007/bf00144482.

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Syarifah, Siti Nur, Erma Musbita Tyastuti, Yasir Sidiq, and Triastuti Rahayu. "Skrining dan Identifikasi Bakteri Selulolitik Tempat Pemakaman Umum." BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains 7, no. 1 (2024): 160–69. http://dx.doi.org/10.31539/bioedusains.v7i1.7838.

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This research aims to determine the potential of cellulolytic bacteria and identify bacterial isolates originating from TPU. A total of 36 bacterial isolates that will be tested for their cellulolytic potential have been isolated from Pracimaloyo Kartasura TPU, Sukoharjo. Selection of cellulolytic bacteria used selective Carboxymethyl Cellulose (CMC) media dripping with 0.1% Congo red while identification was based on colony morphology and Gram staining. The research results showed that 8 bacterial isolates (22.22%) showed positive cellulolytic activity, namely P5, P8, P10, P14, P22, P25, P27,

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Kusumaningrum, Ambar, Ida Bagus Wayan Gunam, and I. Made Mahaputra Wijaya. "OPTIMASI SUHU DAN pH TERHADAP AKTIVITAS ENZIM ENDOGLUKANASE MENGGUNAKAN RESPONSE SURFACE METHODOLOGY (RSM)." JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI 7, no. 2 (2019): 243. http://dx.doi.org/10.24843/jrma.2019.v07.i02.p08.

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This study aims to determine the optimal temperature and pH for the growth of cellulolytic bacterial isolates to produce high cellulase enzyme activity. This study used one potential cellulolytic bacterial isolate B2S8 which had the highest cellulose degradation value and highest cellulase enzyme activity in previous studies. Carboxymethyl Cellulose (CMC) was used as a substrate on growth media and enzyme activity test. Optimization of temperature and pH on cellulase enzyme activity was done by Response Surface Methodology (RSM) by Central Composite Design (CCD). The results using Response Sur

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Anderson, Timothy D., J. Izaak Miller, Henri-Pierre Fierobe, and Robert T. Clubb. "Recombinant Bacillus subtilis That Grows on Untreated Plant Biomass." Applied and Environmental Microbiology 79, no. 3 (2012): 867–76. http://dx.doi.org/10.1128/aem.02433-12.

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ABSTRACTLignocellulosic biomass is a promising feedstock to produce biofuels and other valuable biocommodities. A major obstacle to its commercialization is the high cost of degrading biomass into fermentable sugars, which is typically achieved using cellulolytic enzymes fromTrichoderma reesei. Here, we explore the use of microbes to break down biomass.Bacillus subtiliswas engineered to display a multicellulase-containing minicellulosome. The complex contains a miniscaffoldin protein that is covalently attached to the cell wall and three noncovalently associated cellulase enzymes derived fromC

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Antov, Mirjana, Branimir Jugovic, Milica Gvozdenovic, and Zorica Knezevic-Jugovic. "Partitioning of cellulolytic activity in the polyethylene glycol/dextran two-phase systems." Acta Periodica Technologica, no. 43 (2012): 151–58. http://dx.doi.org/10.2298/apt1243151a.

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This study is concerned with the partitioning of cellulolytic activity in the polyethylene glycol/dextran two-phase systems. In the system of 10% (w/w) polyethylene glycol 1500/5% (w/w) dextran 500,000/80% (w/w) crude enzyme at the pH 5, 100%, yield of cellulolytic activity from Penicillium sp. in the top phase was achieved in a single extraction step. Addition of KH2PO4 to this system at a concentration of 15 mmol/L improved the purification factor in the top phase for cellulolytic activity from crude preparation to a value of 2.6, although it had an adverse effect on the yield in the same ph

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Atwal, A. S., R. M. Teather, S. N. Liss, and F. W. Collins. "Antimicrobial activity of 2-aminophenoxazin-3-one under anaerobic conditions." Canadian Journal of Microbiology 38, no. 10 (1992): 1084–88. http://dx.doi.org/10.1139/m92-177.

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Potential toxicity of 2-aminophenoxazin-3-one to 20 bacterial species and two fungi and its inactivation under anaerobic conditions were investigated. Minimum inhibitory concentration for cellulolytic bacteria was in the range of 50–100 μg∙mL−1, but at 100 μg∙mL−1 of 2-aminophenoxazin-3-one, there was no effect on the growth of any of the noncellulolytic bacteria. Four noncellulolytic bacterial strains showed no inhibition of growth, even at 200 μg∙mL−1 of this compound. Under anaerobic conditions and in the presence of cysteine, the long wavelength absorption band of this compound slowly shif

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Truong, T. Q., and K. D. Nguyen. "Isolation, characterization, and identification of cellulolytic bacteria from household compost for cellulase production." Ukrainian Biochemical Journal 97, no. 1 (2025): 90–102. https://doi.org/10.15407/ubj97.01.090.

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Using microorganisms for waste treatment is a growing trend that offers numerous economic and environmental benefits. This study aimed to evaluate the characteristics of cellulolytic bacterial strains isolated from compost samples from household waste. Biochemical and molecular tests were conducted to identify highly cellulolytic bacteria. Thirty aerobic strains were isolated, of these three isolates (WS1.7, WS3.1, and WS3.29) demonstrated high extracellular cellulase activity and decomposed 51.34–63.43% of the filter paper mass after 7 days of incubation in a liquid medium. Based on their col

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Batubara, U. M., M. Mardalisa, S. Suparjo, H. U. Maritsa, E. Pujianto, and M. Herlini. "Isolation and Characterization of Cellulolytic Bacteria Diversity in Peatland Ecosystem and Their Cellulolytic Activities." IOP Conference Series: Earth and Environmental Science 934, no. 1 (2021): 012028. http://dx.doi.org/10.1088/1755-1315/934/1/012028.

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Abstract Peatlands are terrestrial wetland ecosystems formed from piles of organic matter that decompose into organic deposits. Peat soil has a high potential to produce cellulose which, can be reused by cellulolytic bacteria. This study aims to find out the potential strain of cellulolytic bacteria isolated from peatland ecosystems. The method used was experimental, sequentially, the stages are isolation and screening for cellulolytic bacteria, quantitative testing of cellulolytic activity, characterizing the morphology and physiology of bacteria, and the identification of bacteria based on B

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Helmi, H., R. Kusmiadi, R. G. Mahardika, E. Karsiningsih, L. Nia, and H. Septiani. "Amylolytic, cellulolytic and lactic acid bacteria isolated from porang (Amorphophalus oncophylus) fermented water as potential starter for fermented porang flour." IOP Conference Series: Earth and Environmental Science 1413, no. 1 (2024): 012080. https://doi.org/10.1088/1755-1315/1413/1/012080.

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Abstract Porang tubers could gain economic value through fermentation. A fermentation starter might reduce costs of production. This research aimed to isolate amylolytic, cellulolytic, lactic acid bacteria from spontaneous fermentation of porang tubers that could be further developed into starters in porang flour production. The amylolytic, cellulolytic, lactic acid bacteria were counted and isolated by using starch, CMC, and MRS agar media, respectively. Capability of amylase and cellulase bacteria were measured by using the DNS colorimetric method. Identification of selected microorganisms w

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Rohmatussolihat, Roni Ridwan, Wulansih D. Astuti, et al. "Screening of Lactic Acid Bacteria for Silage Inoculant Candidate." IOP Conference Series: Earth and Environmental Science 1377, no. 1 (2024): 012082. http://dx.doi.org/10.1088/1755-1315/1377/1/012082.

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Abstract Silage fermentation is generally assisted by lactic acid bacteria (LAB). The aim of this study was to screen selected LABs for silage inoculant. A total of 60 LAB isolates from the Indonesian Culture Collection (InaCC) were used in this study. Screening was carried out in an MRS broth medium using three different substrates, which were glucose, sucrose, and carboxyl methyl cellulose (CMC). The supernatant from LAB cultures was tested for pH, lactic acid (LA) concentration, antimicrobial activity, and cellulolytic activity. The results of the screening obtained 4 LAB isolates to be use

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Hastuti, Utami Sri, Kristin Sangur, and Henny Nurul Khasanah. "BIODIVERSITY AND ENZYME ACTIVITY OF INDIGENOUS CELLULOLYTIC AND AMYLOLYTIC BACTERIAS IN DECAYED MANGROVE STEM WASTE PRODUCT AT WAAI SEASHORE, AMBON ISLAND." KnE Life Sciences 2, no. 1 (2015): 433. http://dx.doi.org/10.18502/kls.v2i1.188.

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<p>Mangrove (Sonneratia spp.) could be found at Waai seashore, Ambon Island. The remainder of the mangrove stem will be decayed and become the waste product. Some indigenous bacteria species that live in the decayed mangrove stem waste product have cellulolytic and amylolytic characters. The objectives of this research were to: 1) identify and determine the cellulolytic bacteria species; 2) identify and determine the amylolytic bacteria spesies; 3) determine the cellulolytic bacteria species that have the highest cellulase activity; 4) determine the amylolytic bacteria species that have

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Doolotkeldieva, T. D., and S. T. Bobusheva. "Screening of Wild-Type Fungal Isolates for Cellulolytic Activity." Microbiology Insights 4 (January 2011): MBI.S6418. http://dx.doi.org/10.4137/mbi.s6418.

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Background In this study, wild-type fungal isolates, producing highly effective cellulolytic enzymes were selected for bioconversion of residues and waste from agriculture and rational utilization of energy resources for food production. Methods We screened wild-type fungal isolates of Aspergillus, Penicillium, and Trichoderma with an enhanced ability to produce extracellular cellulase. We carried out solid-state fermentation on a medium of agricultural waste products, including wheat bran, beet peels, and cotton oil cake, as well as additional sources of nitrogen and mineral elements. Enzyme

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Liu, Zhongjiao, Kexuan Ma, Xiujun Zhang, Xin Song, and Yuqi Qin. "Different Putative Methyltransferases Have Different Effects on the Expression Patterns of Cellulolytic Genes." Journal of Fungi 9, no. 11 (2023): 1118. http://dx.doi.org/10.3390/jof9111118.

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Putative methyltranferase LaeA and LaeA-like proteins, conserved in many filamentous fungi, regulate fungal growth, development, virulence, the biosynthesis of secondary metabolites, and the production of cellulolytic enzymes. Penicillium oxaliucm is a typical fungus that produces cellulolytic enzymes. In this study, we reported the biological function of eight putative methyltransferases (PoMtr23C/D/E/F/G/H and PoMtr25A/B) containing a methyltransf_23 or methyltransf_25 domain, with a focus on their roles in the production of cellulolytic enzymes. In P. oxalicum, various methyltransferase gen

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Jannah, Akyunul, Aulanni`am Aulanni`am, Tri Ardyati, and Suharjono Suharjono. "Isolation, Cellulase Activity Test and Molecular Identification of Selected Cellulolytic Bacteria Indigenous Rice Bran." Indonesian Journal of Chemistry 18, no. 3 (2018): 514. http://dx.doi.org/10.22146/ijc.26783.

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Rice bran is the waste product of rice milling which is abundant in Indonesia, it can be used as a raw material for the manufacture of bioethanol by fermentation. Before being fermented, rice bran must be hydrolyzed into glucose by biomass degrading. This study was aimed to isolate indigenous cellulolytic bacteria from rice bran as producer of cellulolytic enzymes and resulted in 22 bacterial isolates that demonstrated cellulolytic activity being identified. Among them, BE 8 and BE 14 isolates showed the highest endoglucanase activity at 2.16 and 1.31 U/mL respectively. Identification of the 1

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Rosmania, Rosmania, Hary Widjajanti, and Elisa Nurnawati. "CELLULASE ACTIVITY OF RIZOSFER BACTERIA OF KETAPANG PLANTS (Terminalia catappa L.)." BIOVALENTIA: Biological Research Journal 9, no. 2 (2023): 123–30. http://dx.doi.org/10.24233/biov.9.2.2023.403.

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Cellulose, a natural biopolymer produced by plants, undergoes enzymatic breakdown by cellulase, cleaving the monomer's β-1,4 glycosidic bonds. Cellulolytic bacteria, producers of cellulase, can be isolated from plant rhizospheres, like Ketapang (Terminalia catappa L.), abundantly found in the Biology Department at Universitas Sriwijaya, South Sumatra, Indonesia. This study aimed to isolate cellulolytic bacteria from Ketapang rhizosphere soil and assess their cellulase activity. The spread plate method on selective Carboxy Methyl Cellulose Agar (CMC agar) was used for bacterial isolation, and c

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Lusiana, Helga, MG Isworo Rukmi, and Budi Raharjo. "Eksplorasi Jamur Alkalotoleran dari Desa Sukolilo Barat, Kecamatan Labang, Kabupaten Bangkalan, Madura, Jawa Timur." Bioma : Berkala Ilmiah Biologi 16, no. 1 (2014): 10. http://dx.doi.org/10.14710/bioma.16.1.10-17.

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Mold exploration from limestone hills West Sukolilo village, District Labang, Bangkalan, Madura, East Java, conducted to found the alkalotolerant mold isolates and investigate their amylolytic, proteolytic, and cellulolytic activity. Mold isolation were done using spread plate and dilution method on PDA and CMA at pH 8.0, 9.0, and 10.0. Twenty-seven mold isolates have been found consist of four genus i.e. Aspergillus, Fusarium, Curvularia, and Trichoderma. The result of amylolytic test showed Trichoderma longibrachiatum (IMD-26) have the highest activity at pH 8, while Fusarium sp 2 (IMD-24) s

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Phitsuwan, Moraïs, Dassa, Henrissat, and Bayer. "The Cellulosome Paradigm in An Extreme Alkaline Environment." Microorganisms 7, no. 9 (2019): 347. http://dx.doi.org/10.3390/microorganisms7090347.

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Rapid decomposition of plant biomass in soda lakes is associated with microbial activity of anaerobic cellulose-degrading communities. The alkaliphilic bacterium, Clostridium alkalicellulosi, is the single known isolate from a soda lake that demonstrates cellulolytic activity. This microorganism secretes cellulolytic enzymes that degrade cellulose under anaerobic and alkaliphilic conditions. A previous study indicated that the protein fraction of cellulose-grown cultures showed similarities in composition and size to known components of the archetypical cellulosome Clostridium thermocellum. Bi

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Satwika, Taruna Dwi, Hendro Pramono, and Dwiana Muflihah Yulianti. "Potensi Isolat Bakteri Selulolitik Toleran Panas Asal Tanah, Sampah Dapur, dan Kotoran Sapi Dalam Biodegradasi Serasah Daun." Al-Kauniyah: Jurnal Biologi 16, no. 2 (2023): 327–35. http://dx.doi.org/10.15408/kauniyah.v16i2.25616.

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AbstrakBakteri selulolitik memainkan peranan penting dalam biodegradasi komponen selulosa pada sampah organik. Namun, proses pengomposan umumnya melewati fase termofilik (suhu mencapai 55 °C), sehingga tidak semua bakteri dapat bertahan. Sebanyak delapan isolat bakteri selulolitik telah berhasil diisolasi dari tanah, sampah dapur, dan kotoran sapi. Namun, isolat-isolat tersebut belum diketahui aktivitas selulolitiknya pada suhu tinggi dan kemampuannya dalam mendegradasi biomassa serasah daun. Tujuan penelitian ini adalah mengetahui aktivitas selulolitik isolat bakteri pada suhu tinggi secara k

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Dewiyanti, Irma, Darmawi Darmawi, Zainal Abidin Muchlisin, et al. "Cellulase activity of bacteria isolated from water of mangrove ecosystem in Aceh Province." Depik 10, no. 3 (2021): 243–50. http://dx.doi.org/10.13170/depik.10.3.22964.

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Cellulolytic bacteria that produce cellulase enzymes play an essential role in degrading cellulose in their habitat. The presence of cellulolytic bacteria strongly supports the fertility and productivity in mangrove waters. The objectives of the study are to analyze the activity of cellulase enzyme qualitatively through the cellulolytic index and quantitatively through the activity and specific activity of the cellulase enzyme from bacteria isolated from the water of mangrove ecosystems in Aceh Province. The qualitative experiment of enzyme activity was carried out at the Microbiology laborato

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Kuz'min, P. N. "XYLOTROPHIC FUNGUS Trichoderma atroviride: CULTIVATION, EXTRACELLULAR HYDROLYTIC AND ANTIMICROBIAL ACTIVITY." Biotechnologia Acta 14, no. 3 (2021): 46–53. http://dx.doi.org/10.15407/biotech14.03.046.

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Xylotrophic fungi are well known by their ability to excrete enzymes into environment. These fungi have important biotechnological potential and some of them produce industrial enzymes. Besides, xylotrophic fungal species have recently attracted a lot of attention among researchers as a source of antibacterial drugs. Aim. To analyze the effect of the carbon source in the culture medium, as well as the conditions of deep cultivation on the mycelium yield, proteolytic, cellulolytic and antimicrobial activity of the culture liquid of Trichoderma atroviride. Methods. Deep culture methods were used

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Bhat, Siva, R. J. Wallace, and E. R. Ørskov. "Study of the relation between straw quality and its colonization by rumen micro-organisms." Journal of Agricultural Science 110, no. 3 (1988): 561–65. http://dx.doi.org/10.1017/s0021859600082137.

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SummaryThe relation between the dry-matter degradability (DMD) of different cultivars of barley straw and their colonization by rumen micro-organisms was studied in incubations of straw with the mixed populationin situand with pure cultures of rumen bacteriain vitro.The total attached populationin situwas quantified by particle-associated i glutamate dehydrogenase (GDH) activity, while the cellulolytic population was assessed by bound carboxymethylcellulase (CMCase) activity. CMCase reached a peak between 24 and 36 h incubation, and the peak activity was highly correlated with DMD between 24 )

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Sartori, Tanara, Heloisa Tibolla, Elenizi Prigol, Luciane Maria Colla, Jorge Alberto Vieira Costa, and Telma Elita Bertolin. "Enzymatic Saccharification of Lignocellulosic Residues by Cellulases Obtained from Solid State Fermentation UsingTrichoderma viride." BioMed Research International 2015 (2015): 1–9. http://dx.doi.org/10.1155/2015/342716.

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The aim of this study was to verify the viability of lignocellulosic substrates to obtain renewable energy source, through characterization of the cellulolytic complex, which was obtained by solid state fermentation usingTrichoderma viride. Enzymatic activity of the cellulosic complex was measured during saccharification of substrates filter paper, eucalyptus sawdust, and corncob, and compared with the activity of commercial cellulase. The characterization of the enzymes was performed by a 22Full Factorial Design, where the pH and temperature were the variables of study. Enzymatic saccharifica

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Nababan, Monalisa, Ida Bagus Wayan Gunam, and I. Made Mahaputra Wijaya. "PRODUKSI ENZIM SELULASE KASAR DARI BAKTERI SELULOLITIK." JURNAL REKAYASA DAN MANAJEMEN AGROINDUSTRI 7, no. 2 (2019): 190. http://dx.doi.org/10.24843/jrma.2019.v07.i02.p03.

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The purpose of this research was to elucidate the potentials of cellulolytic bacteria isolates in producing crude cellulose enzymes and to determine the ability of exoglucanase enzymes and endoglucanase enzymes produced from selected cellulolytic bacteria isolates. Ten potential isolates from previous research were confirmed using 1% Carboxymethyl Cellulose (CMC) media with the addition of Congo red and four potential isolates were obtained which then crude cellulose production and enzyme activity tests were carried out using cellulose substrates such as Carboxymethyl Cellulose (CMC), and Filt

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Purwanto, Agus. "Isolasi Jamur Selulolitik Trichoderma pada Beberapa Limbah Organik." JURNAL AGRI-TEK : Jurnal Penelitian Ilmu-Ilmu Eksakta 21, no. 1 (2020): 42–47. http://dx.doi.org/10.33319/agtek.v21i1.70.

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Cellulolytic fungi have an important role for the largest material flow in the biosphere. Trichoderma fungi are ubiquitous and have the ability to degrade cellulose. The study was conducted to isolate dan screen Trichoderma cellulolytic fungi from soil heaps of some organic waste. Soil samples were taken from soil heaps of some organic waste to isolate Trichoderma cellulolytic fungi. The sampling method is carried out randomly from top soil layers of organic waste litter (bagasse, sugar cane leaf, rice straw, and garbage). Isolation and screening of Trichoderma fungi are determined based on ob

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Manoliu, Al, L. Oprica, Z. Olteanu, et al. "Peroxidase activity in magnetically exposed cellulolytic fungi." Journal of Magnetism and Magnetic Materials 300, no. 1 (2006): e323-e326. http://dx.doi.org/10.1016/j.jmmm.2005.10.111.

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