To see the other types of publications on this topic, follow the link: Cement germ.
Journal articles on the topic 'Cement germ'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 34 journal articles for your research on the topic 'Cement germ.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.
1
Reunov, Arkadiy A., Doris W. T. Au, Yana N. Alexandrova, Michael W. L. Chiang, Miles T. Wan, Konstantin V. Yakovlev, Yulia A. Reunova, et al. "Germ plasm-related structures in marine medaka gametogenesis; novel sites of Vasa localization and the unique mechanism of germ plasm granule arising." Zygote 28, no. 1 (October 8, 2019): 9–23. http://dx.doi.org/10.1017/s0967199419000546.
Full textAbstract:
SummaryGerm plasm, a cytoplasmic factor of germline cell differentiation, is suggested to be a perspective tool for in vitro meiotic differentiation. To discriminate between the: (1) germ plasm-related structures (GPRS) involved in meiosis triggering; and (2) GPRS involved in the germ plasm storage phase, we investigated gametogenesis in the marine medaka Oryzias melastigma. The GPRS of the mitosis-to-meiosis period are similar in males and females. In both sexes, five events typically occur: (1) turning of the primary Vasa-positive germ plasm granules into the Vasa-positive intermitochondrial cement (IMC); (2) aggregation of some mitochondria by IMC followed by arising of mitochondrial clusters; (3) intramitochondrial localization of IMC-originated Vasa; followed by (4) mitochondrial cluster degradation; and (5) intranuclear localization of Vasa followed by this protein entering the nuclei (gonial cells) and synaptonemal complexes (zygotene–pachytene meiotic cells). In post-zygotene/pachytene gametogenesis, the GPRS are sex specific; the Vasa-positive chromatoid bodies are found during spermatogenesis, but oogenesis is characterized by secondary arising of Vasa-positive germ plasm granules followed by secondary formation and degradation of mitochondrial clusters. A complex type of germ plasm generation, ‘the follicle cell assigned germ plasm formation’, was found in late oogenesis. The mechanisms discovered are recommended to be taken into account for possible reconstruction of those under in vitro conditions.
2
Reunov, Arkadiy. "Structures related to the germ plasm in mouse." Zygote 14, no. 3 (August 2006): 231–38. http://dx.doi.org/10.1017/s0967199406003789.
Full textAbstract:
SummaryThis report presents data from ultrastructural and morphometric studies on the germinal-body-like structures, nuage, nuage–mitochondrial clusters and chromatoid bodies in 4.5-day embryo cells and spermatogenic cells of the laboratory mouse Mus musculus. In the 4.5-day embryo cells the germinal-body-like structures that, according to previous data, arise by condensation of mitochondria in Graafian oocytes, were found not to undergo any ultrastructural alterations. In spermatogonia the germinal-body-like structures presumably were transformed into nuage that functioned as ‘intermitochondrial cement’ binding the mitochondrial clusters. In primary spermatocytes mitochondria aggregated by nuage were found with large vacuoles containing membraneous conglomerates that were obviously excreted by organelles into the cytoplasm. The chromatoid bodies that arose in spermatocytes and finally disintegrated in the posterior part of late spermatids seemed not to be implicated in the pathway of the germinal-body-like structure. The dispersion of chromatoid bodies was noted to be accompanied by excretion of membraneous conglomerates by late spermatid mitochondria. The spermatozoa were not found to contain either the germinal-body-like structures or any other germ-plasm-related structures.
3
Wang, Xiaoli, Chunyu Lv, Ying Guo, and Shuiqiao Yuan. "Mitochondria Associated Germinal Structures in Spermatogenesis: piRNA Pathway Regulation and Beyond." Cells 9, no. 2 (February 10, 2020): 399. http://dx.doi.org/10.3390/cells9020399.
Full textAbstract:
Multiple specific granular structures are present in the cytoplasm of germ cells, termed nuage, which are electron-dense, non-membranous, close to mitochondria and/or nuclei, variant size yielding to different compartments harboring different components, including intermitochondrial cement (IMC), piP-body, and chromatoid body (CB). Since mitochondria exhibit different morphology and topographical arrangements to accommodate specific needs during spermatogenesis, the distribution of mitochondria-associated nuage is also dynamic. The most relevant nuage structure with mitochondria is IMC, also called pi-body, present in prospermatogonia, spermatogonia, and spermatocytes. IMC is primarily enriched with various Piwi-interacting RNA (piRNA) proteins and mainly functions as piRNA biogenesis, transposon silencing, mRNA translation, and mitochondria fusion. Importantly, our previous work reported that mitochondria-associated ER membranes (MAMs) are abundant in spermatogenic cells and contain many crucial proteins associated with the piRNA pathway. Provocatively, IMC functionally communicates with other nuage structures, such as piP-body, to perform its complex functions in spermatogenesis. Although little is known about the formation of both IMC and MAMs, its distinctive characters have attracted considerable attention. Here, we review the insights gained from studying the structural components of mitochondria-associated germinal structures, including IMC, CB, and MAMs, which are pivotal structures to ensure genome integrity and male fertility. We discuss the roles of the structural components in spermatogenesis and piRNA biogenesis, which provide new insights into mitochondria-associated germinal structures in germ cell development and male reproduction.
4
Guven, Esra Pamukcu, Mehmet Emir Yalvac, Fikrettin Sahin, Munevver M. Yazici, Albert A. Rizvanov, and Gunduz Bayirli. "Effect of Dental Materials Calcium Hydroxide–containing Cement, Mineral Trioxide Aggregate, and Enamel Matrix Derivative on Proliferation and Differentiation of Human Tooth Germ Stem Cells." Journal of Endodontics 37, no. 5 (May 2011): 650–56. http://dx.doi.org/10.1016/j.joen.2011.02.008.
Full text
5
Xie, H., N. Dubey, W. Shim, C. J. A. Ramachandra, K. S. Min, T. Cao, and V. Rosa. "Functional Odontoblastic-Like Cells Derived from Human iPSCs." Journal of Dental Research 97, no. 1 (September 12, 2017): 77–83. http://dx.doi.org/10.1177/0022034517730026.
Full textAbstract:
The induced pluripotent stem cells (iPSCs) have an intrinsic capability for indefinite self-renewal and large-scale expansion and can differentiate into all types of cells. Here, we tested the potential of iPSCs from dental pulp stem cells (DPSCs) to differentiate into functional odontoblasts. DPSCs were reprogrammed into iPSCs via electroporation of reprogramming factors OCT-4, SOX2, KLF4, LIN28, and L-MYC. The iPSCs presented overexpression of the reprogramming genes and high protein expressions of alkaline phosphatase, OCT4, and TRA-1-60 in vitro and generated tissues from 3 germ layers in vivo. Dentin discs with poly-L-lactic acid scaffolds containing iPSCs were implanted subcutaneously into immunodeficient mice. After 28 d from implantation, the iPSCs generated a pulp-like tissue with the presence of tubular dentin in vivo. The differentiation potential after long-term expansion was assessed in vitro. iPSCs and DPSCs of passages 4 and 14 were treated with either odontogenic medium or extract of bioactive cement for 28 d. Regardless of the passage tested, iPSCs expressed putative markers of odontoblastic differentiation and kept the same mineralization potential, while DPSC P14 failed to do the same. Analysis of these data collectively demonstrates that human iPSCs can be a source to derive human odontoblasts for dental pulp research and test bioactivity of materials.
6
Brandariz, Rodrigo, Javier Bennice, Jorge Boretto, Ezequiel Zaidenberg, Pablo De Carli, and Gerardo Gallucci. "Clavo endomedular recubierto con antibiótico para controlar la infección en una seudoartrosis infectada de húmero. [Antibiotic cement rod to control infection in infected humerus nonunion]." Revista de la Asociación Argentina de Ortopedia y Traumatología 84, no. 2 (May 2, 2019): 90–98. http://dx.doi.org/10.15417/issn.1852-7434.2019.84.2.849.
Full textAbstract:
Objetivo: Comunicar la eficacia del clavo endomedular recubierto con antibiótico (CERA) para erradicar la infección en la seudoartrosis infectada de húmero (SIH).Materiales y Métodos: Once pacientes (edad promedio 48 años). El tiempo entre la fractura y la cirugía fue 25 meses. El CERA se impregnó con vancomicina en 9 pacientes. El seguimiento promedio fue de 54 meses.Resultados: Se aisló S. aureus resistente a meticilina (SARM) en 5 pacientes. Todos recibieron antibióticos sistémicos por 7 semanas. El antibiótico más utilizado fue vancomicina. La mediana entre el primer tiempo quirúrgico y la reconstrucción fue 56 días (RIC 47-98). Luego del desbridamiento quirúrgico del primer tiempo, se midió el defecto óseo remanente y se lo dividió con variables dicotómicas: grupo con defectos <2 cm (7 pacientes) y grupo con defectos ≥2 cm (4 pacientes). No se observaron diferencias significativas entre la mediana de días entre el primero y segundo tiempo quirúrgico comparando el desarrollo de SARM con el de otros gérmenes (48 días [RIC 45-75] vs. 73,5 días [RIC 56-149], p = 0,2002 Mann-Whitney), ni en la proporción del tamaño del defecto óseo según el desarrollo de SARM o de otro germen (60% vs. 17%, p = 0,242 Fisher). Todos los cultivos fueron negativos y se logró la consolidación del foco fracturario, sin recurrencia de la infección.Conclusiones: El CERA es una buena opción terapéutica en el primer tiempo quirúrgico para un paciente con SIH. Se pudo controlar la infección, lo que permitió la reconstrucción secundaria de la seudoartrosis. AbstractObjetive: To evaluate the efficiency of the antibiotic cement rod (ACR) in the eradication of infection in infected humerusnonunion (IHN).Material and methods: We included 11 patients with IHN with a mean age of 48 years. The time between fracture-surgery was 25 months. The ACR was impregnated with vancomycin in 9 of de 11 cases. Follow-up was 54 months.Results: Methicillinresistant staphylococcus aureus (MRSA) was isolated in 5 of cases. All patients received antibiotics systemically for 7 weeks. Vancomycin was the most commonly used antibiotic. Time between ACR and reconstructive surgery averaged 56 days [confidence interval range (CIR) 47-98]. After debridement and implant removal, the residual space of the nonunion was measured with dichotomous variables and classified into two groups: group 1, < 2 cm (7 patients) and group 2, ≥2 cm (4 patients). No significant differences were observed between the number of days in which the ACR was placed and the development of the SAMR as compared to other germs [48 days (CIR 45-75) vs. 73 days (CIR 56-149) p= 0.2002 Mann Whitney]. Nor were differences observed in the size of the defect in those who developed MRS or any other germ (p=0.242 Fisher). Reconstruction was performed with different techniques. Laboratory parameters were normal, cultures were negative. Fractures could be consolidated without infection recurrence.Conclusions: ACR is a good treatment option for a patient with an INH. The infection could be controlled in all of the cases, which allowed the secondary reconstruction of the nonunion
7
Clauss, Martin, Esther Laschkolnig, Susanne Graf, and Klaus-Dieter Kühn. "Influence of Sonication on Bacterial Regrowth from Antibiotic Loaded PMMA Scaffolds - An In-vitro Study." Journal of Bone and Joint Infection 2, no. 4 (November 17, 2017): 213–17. http://dx.doi.org/10.7150/jbji.22382.
Full textAbstract:
Abstract. Periprosthetic joint infection (PJI) is one of the most challenging complications after joint replacement. However, when treated correctly, chances of recovery are good. The most important step in correct diagnosis and management of PJI is the detection of the causative germ. In the last years, the use of sonication in the diagnostic process has become more important. However, this diagnostic methodology has been controversially discussed when used in combination with antibiotic loaded bone cement (PMMA), which is frequently used in joint replacement surgeries. The aim of this study was thus to analyse in vitro bacterial growth in sonication fluid cultures obtained from antibiotic loaded PMMA which were contaminated with various bacterial biofilms.Sonication fluid obtained from antibiotic loaded PMMA (Copal G+V and Copal G+C) and plain Palacos R (control) contaminated either with S. aureus, E. faecalis, S. sanguinis or P.acnes, were analysed for bacterial re-growth in a standardised in-vitro setting.In vitro bacterial growth was not interfered by released antibiotics from sonication of antibiotic loaded PMMA for S. aureus, E. faecalis and S. sanguinis. However, for P. acnes bacterial counts were affected by the released antibiotics as well as by the time delay between sonication and analysis. The in-vitro data suggest sonication to be an easy and sensitive diagnostic modality to detect easy-to-detect bacteria, however, results are alarming for the difficult-to-detect bacteria P. acnes, indicating that further attention and research is necessary to improve the detection of difficult-to-detect bacteria.
8
Krówczyńska, Małgorzata, Ewa Wilk, Piotr Pabjanek, and Marlena Kycko. "Hyperspectral discrimination of asbestos‑cement roofing." Geomatics and Environmental Engineering 11, no. 1 (2017): 47. http://dx.doi.org/10.7494/geom.2017.11.1.47.
Full text
9
Liu, Wan-Sheng, Yaqi Zhao, Chen Lu, Gang Ning, Yun Ma, Francisco Diaz, and Michael O'Connor. "A novel testis-specific protein, PRAMEY, is involved in spermatogenesis in cattle." Reproduction 153, no. 6 (June 2017): 847–63. http://dx.doi.org/10.1530/rep-17-0013.
Full textAbstract:
Preferentially expressed antigen in melanoma (PRAME) is a cancer/testis antigen that is predominantly expressed in normal testicular tissues and a variety of tumors. The function of the PRAME family in spermatogenesis remains unknown. This study was designed to characterize the Y-linked PRAME (PRAMEY) protein during spermatogenesis in cattle. We found that PRAMEY is a novel male germ cell-specific, and a germinal granule-associated protein that is expressed in spermatogenic cells during spermatogenesis. The intact PRAMEY protein (58 kDa) was detected in different ages of testes but not in epididymal spermatozoa. A PRAMEY isoform (30 kDa) was highly expressed only in testes after puberty and in epididymal spermatozoa. This isoform interacts with PP1γ2 and is likely the mature protein present in the testes and sperm. Immunofluorescent staining demonstrated that PRAMEY was located predominantly in the acrosome granule of spermatids, and in acrosome and flagellum of spermatozoa. Immunogold electron microscopy further localized the PRAMEY protein complex to the nucleus and several cytoplasmic organelles, including the rough endoplasmic reticulum, some small vesicles, the intermitochondrial cement, the chromatoid body and the centrioles, in spermatogonia, spermatocytes, spermatids and/or spermatozoa. PRAMEY was highly enriched in and structurally associated with the matrix of the acrosomal granule (AG) in round spermatids, and migrated with the expansion of the AG during acrosomal biogenesis. While the function of PRAMEY remains unclear during spermatogenesis, our results suggest that PRAMEY may play an essential role in acrosome biogenesis and spermatogenesis.Free Chinese abstract: A Chinese translation of this abstract is freely available athttp://www.reproduction-online.org/content/153/6/847/suppl/DC2Spanish abstract: A Spanish translation of this abstract is freely available athttp://www.reproduction-online.org/content/153/6/847/suppl/DC3
10
Książek, Judyta. "Methods for detection of asbestos-cement roofing sheets." Geomatics and Environmental Engineering 8, no. 3 (2014): 59. http://dx.doi.org/10.7494/geom.2014.8.3.59.
Full text
11
Lapera, Miguel, Facundo Gigante, Manuel Perez Zabala, Esteban Adjigogovic, Francisco Sylvester, Juan Manuel Torres Moirano, and Emanuel Fedun Rodriguez. "Mucormicosis after Anterior Cruciate Ligament (ACL) Reconstruction." Orthopaedic Journal of Sports Medicine 6, no. 12_suppl5 (December 1, 2018): 2325967118S0020. http://dx.doi.org/10.1177/2325967118s00202.
Full textAbstract:
We hereby present a case of a 23 year old male patient with an Anterior Cruciate Ligament (ACL) reconstruction with autologous hamstrings graft and fixation with Peek implants. He attended our service 25 days after surgery with intense and persistent knee pain, periarticular effusion, edema, increased local temperature and poor wound drainage. The most striking symptom was the persistence of pain that did not yield with opioid analgesics. Upon suspicion of infection, a routine lab study was performed. Serial blood cultures, X-Rays, CT, and knee MRI were taken. The lab results showed leukocytosis with a 16,500 count, in predominance of Neutrophils 76.8%., and ESR increase, with negative blood cultures. The decided procedure was to perform knee arthrocentesis and bacteriological and histochemical study with germ typification. Fungal infection was suspected and specific study was requested with an Anatomopatologist. Examination with special histochemical technique, GROCCOTT, PAS and GIEMSA was performed, showing numerous fungal elements (thick and branched hyphae). Morphological findings are linked to Rhizopus infection (Mucormycosis) NECROTIZING MYOCTIC ACUTE OSTEOMYELITIS. Surgical treatment: Radical debridement with removal of surgical material. Arthrotomy and enlarged synovectomy in the suprapatellar recess. We proceeded to carve a window on the anterior distal femur of 6 x 4 cm with wide curettage and cavity filling with cement beads impregnated in Amphotericin. Closed drain for 48 Hs. The Department of Infectious Diseases proceeded to perform parenteral treatment with Amphotericin B EV 1 mg / kg for 6 weeks (After 4 rotated Amphotericin B Liposomal because of nephrotoxicity). Postoperative control with MRI, CT and Lab. Biopsy 45 days after surgery. Negative bone culture and biopsy under direct vision with the presence of Anatomopatologist in the operating room with removal of cement beads with Amphotericin. The diaphysis of the femur is covered only with aponeurotic tissue, without bone grafts. For 4 months he proceeded to walk with crutches without load bearing. At 12 months, lab control, without abnormalities, knee without effusion, slight deficit of flexion and extension, stable, with negative semiology, anterior drawer and pivot shift tests were negative and complementary studies of RNM and X-Rays only showed femoral diaphysis with bone rarefaction and secondary infection. Bacteriological and laboratory without particularity considering the clinical picture in a definitive high way. CONCLUSION: Mucormycosis is a serious infection caused by fungi of the order Mucorales, of which the most important family is that of the Mucoraceae, which includes the genera Rhizopus, Mucor and Absidia. Low incidence infection, but with a high rate of morbidity and mortality. It is vital to make an early diagnosis, for which one has to maintain a high rate of clinical suspicion, this being the most fulminant mycotic disease in human beings. Our purpose is to present a case with very particular characteristics, which differ both in the clinical presentation and in the first lab results with the typical bacterial infection of the ACL postoperative knee.
12
Morejón Alonso, Lizette, Iran Fernández Torres, Ángela M. Zayas Tamayo, Oscar Ernesto Ledea Lozano, Ivette Durán Ramos, José Ángel Delgado García-Menocal, Nely Rios-Donato, and Eduardo Mendizábal. "Antibacterial effect of acrylic bone cements loaded with drugs of different action’s mechanism." Journal of Infection in Developing Countries 13, no. 06 (June 30, 2019): 487–95. http://dx.doi.org/10.3855/jidc.10716.
Full textAbstract:
Introduction: Antibiotic-loaded bone cements of poly(methyl methacrylate) are considered as very useful biomaterials for the management of corporal deep osseous infections. However, the high prevalence of resistant germs and polymicrobial infections makes it necessary to search for new formulations of bone cements containing antibiotics for local antibacterial therapy. In this work, bone cements loaded with drugs with different mechanism of action were evaluated to determine its antibacterial effectiveness on Pseudomonas aeruginosa.
Methodology: Poly(methyl methacrylate) cements loaded with 10 wt.% of Oleozon®, mixtures of Ciprofloxacin/Meropenem and Ciprofloxacin/Meropenem/Oleozon® were prepared. The in vitro drugs release in water was followed by UV-Vis spectroscopy, and their antibacterial activity against Pseudomonas aeruginosa was evaluated for 11 days using the microdilution method.
Results: All the extracts demonstrated an inhibitory effect on the growth of the strain during the whole trial period. Extracts from cement with Oleozon® only presented a total antibacterial inhibitory effect during 20 hours for the extracts taken at day 1 while the extracts from the cements loaded with mixtures of Ciprofloxacin/Meropenem and Ciprofloxacin/Meropenem/Oleozon® showed complete inhibition of the growth of the microorganism, even at 11 days. At the end of the trial period, some of the drugs remained inside the matrices, indicating that they can be released for a longer time in treatments.
Conclusions: The results indicated a positive antibacterial effect by the combined used of the two or the three drugs tested against the Gram-negative bacilli Pseudomonas aeruginosa, so these proposal may be a valid alternative to be considered by surgeons.
13
Maharani, Nuni, Agung Wibowo, Dudi Aripin, and Mohammad Richata Fadil. "Perbedaan nilai kekerasan permukaan semen Glass Ionomer (GIC) dan modifikasi resin semen Glass Ionomer (RMGIC) akibat efek cairan lambung buatan secara in vitro." Padjadjaran Journal of Dental Researchers and Students 1, no. 2 (October 30, 2017): 77. http://dx.doi.org/10.24198/pjdrs.v1i1.22293.
Full textAbstract:
Pendahuluan: Gastroesophageal reflux disease (GERD) didefinisikan sebagai reflex otot esophagus atau spincter, yang memungkinkan asam lambung bergerak naik melalui kerongkongan hingga masuk kedalam rongga mulut. Asam lambung memiliki pH berkisar 1 hingga 1.5, berada di bawah pH kritis email sebesar 5.5 yang dapat menyebabkan terjadinya demineralisasi email, dentin, dan sementum. Tujuan penelitian adalah untuk membedakan nilai kekasaran permukaan semen glass ionomer (GIC) dan modifikasi resin semen glass ionomer (RMGIC) akibat efek cairan lambung buatan. Metode: Penelitian yang dilakukan merupakan penelitian eksperimental murni secara in vitro. Sampel dipersiapkan sebanyak 40 buah, yang terdiri dari 20 sampel semen glass ionomer (GIC) dan 20 sampel modifikasi resin semen glass ionomer (RMGIC). Seluruh sampel dibagi ke dalam 4 kelompok, masing-masing terdiri dari 10 sampel yaitu (1) kelompok GIC direndam dalam saliva buatan, (2) kelompok RMGIC direndam dalam saliva buatan, (3) kelompok GIC direndam dalam cairan lambung buatan selama 3 kali 7 menit dan setelahnya direndam kembali dalam saliva buatan, dan (4) kelompok RMGIC direndam dalam cairan lambung buatan selama 3 kali 7 menit dan setelahnya direndam kembali dalam saliva buatan. Perendaman dilakukan selama 9 hari. Nilai kekasaran diukur dengan menggunakan surface roughness tester (Profilometri). Hasil dianalisis secara statistik dengan menggunakan uji ANAVA dan analisis post-hoc dengan menggunakan t-test. Hasil: Terdapat perbedaan nilai kekasaran permukaan semen glass ionomer (GIC) dan modifikasi resin semen glass ionomer (RMGIC) akibat efek cairan lambung buatan. Simpulan: Paparan cairan asam lambung pada pasien GERD dapat mempengaruhi kekasaran permukaan bahan restorasi GIC dan RMGIC. Kata kunci: Gastroesophageal reflux disease, Kekasaran permukaan, semen glass ionomer, modifikasi resin semen glass ionomer, profilometri ABSTRACTIntroduction: Gastroesophageal reflux disease (GERD) is defined as involuntary muscle relaxing of the upper esophageal sphincter, which allows refluxed acid to move upward through the esophagus into the oral cavity. The gastric acid has pH between 1 and 1.5, far below the critical pH of 5.5 at which tooth enamel will dissolve. Gastric juice has been shown to demineralize enamel, dentin, and root cementum. Methods: Fourthy samples of each restorative material, a conventional glass ionomer cement (20 samples) and a resin modified glass ionomer cement (20 samples), were prepared and divided into four groups, each group consist of 10 samples. Group (1) group of GIC immersed in simulated saliva and group (2) group of RMGIC immersed in simulated saliva, both control groups immersed for 9 days. Group (3) were group GIC and group (4) were RMGIC, both groups immersed in simulated saliva for 9 days and in between both groups immersed in gastric juice every 3 times a day for 7 minute. Each group subjected to profilometric analysis. The profilometric values were statistically analyzed using ANOVA and 2-way analysis of variance (post-hoc). Results: There are differences between GIC and RMGIC after immersion in gastric juice. Conclusion: Surface roughness of all tested materials were affected by the simulated gastric juice.Keywords: Gastroesophageal reflux disease, surface roughness, glass ionomer cement, resin modified glass ionomer cement, profilometer
14
Oleniacz, Robert, and Magdalena Kasietczuk. "Co-incineration of large quantities of alternative fuels in a cement kiln - the problem of air pollutant emissions." Geomatics and Environmental Engineering 6, no. 4 (2012): 47. http://dx.doi.org/10.7494/geom.2012.6.4.47.
Full text
15
Guven, Esra Pamukcu, Mehmet Emir Yalvac, Mehmet Baybora Kayahan, Hakk Sunay, Fikrettin SahIn, and Gunduz Bayirli. "Human tooth germ stem cell response to calcium-silicate based endodontic cements." Journal of Applied Oral Science 21, no. 4 (July 2013): 351–57. http://dx.doi.org/10.1590/1678-775720130047.
Full text
16
Yamaji, Masashi, Takashi Tanaka, Mayo Shigeta, Shinichiro Chuma, Yumiko Saga, and Mitinori Saitou. "Functional reconstruction of NANOS3 expression in the germ cell lineage by a novel transgenic reporter reveals distinct subcellular localizations of NANOS3." REPRODUCTION 139, no. 2 (February 2010): 381–93. http://dx.doi.org/10.1530/rep-09-0373.
Full textAbstract:
Mutations of RNA-binding proteins such as NANOS3, TIAL1, and DND1 in mice have been known to result in the failure of survival and/or proliferation of primordial germ cells (PGCs) soon after their fate is specified (around embryonic day (E) 8.0), leading to the infertility of these animals. However, the mechanisms of actions of these RNA-binding proteins remain largely unresolved. As a foundation to explore the role of these RNA-binding proteins in germ cells, we established a novel transgenic reporter strain that expresses NANOS3 fused with EGFP under the control of Nanos3 regulatory elements. NANOS3–EGFP exhibited exclusive expression in PGCs as early as E7.25, and continued to be expressed in female germ cells until around E14.5 and in male germ cells throughout the fetal period with declining expression levels after E16.5. NANOS3–EGFP resumed strong expression in postnatal spermatogonia and continued to be expressed in undifferentiated spermatogonial cells in adults. Importantly, the Nanos3–EGFP transgene rescued the sterile phenotype of Nanos3 homozygous mutants, demonstrating the functional equivalency of NANOS3–EGFP with endogenous NANOS3. We found that throughout germ cell development, a predominant amount of NANOS3–EGFP co-localized with TIAL1 (also known as TIAR) and phosphorylated eukaryotic initiation factor 2α, markers for the stress granules, whereas a fraction of it showed co-localization with DCP1A, a marker for the processing bodies. On the other hand, NANOS3–EGFP did not co-localize with Tudor domain-containing protein 1, a marker for the intermitochondrial cements, in spermatogenic cells. These findings unveil the presence of distinct posttranscriptional regulations in PGCs soon after their specification, for which RNA-binding proteins such as NANOS3 and TIAL1 would play critical functions.
17
Yang, Z. X., Kyu Hong Hwang, Jeong Bae Yoon, J. O. Kim, and M. C. Kim. "Properties of Permeable Concrete Substrate Having Dual Pore Structures." Materials Science Forum 569 (January 2008): 233–36. http://dx.doi.org/10.4028/www.scientific.net/msf.569.233.
Full textAbstract:
Porous concretes with continuous voids have been gaining more interest as an ecological material because of their useful functions such as water permeability and adsorption ability. Especially, to make porous concrete much more environmentalized, micropores play a role of nest for microorganism and germs to live in. So micropore distribution and the size of micropores especially open pores are the key point . In this study, the size and distribution of micropores of porous concrete were effected by the AE agents to the cement pastes and then by the treating types, treating times, treating temperatures, and the vacuum pressure during the treating. And another group specimens were added sodium bicarbonate and alum. And then physical properties were examined, digital microscope was also used to observe the micropores sizes and distributions. Cracks were observed on the cement-aggregate joint when the amount and vacuum pressure increased. And high treating temperature shows no good on the bonding of cement paste and aggregate.
18
Picos, Andrei, Andreea L. Rachisan, and Alexandra Dadarlat. "Minimally Invasive Dental Treatment Using Composites and Ceramics in GERD Diagnoses Patients." Materiale Plastice 55, no. 2 (June 30, 2018): 252–54. http://dx.doi.org/10.37358/mp.18.2.5004.
Full textAbstract:
Specialty literature demonstrates the direct link between gastro-esophageal reflux diseases (GERD) and dental errosion (DE). Patients diagnosed with GERD often shown dental tissue loss in the enamel and dentine, somentimes going as far as opening the pulp chamber. Modern minimal invasive treatments make use of the current performance of cements and prosthetic materials (composites and ceramics) to conserve healthy enamel and dentine, in comparison to classic reconstruction methods.
19
Yabuta, Yukihiro, Hiroshi Ohta, Takaya Abe, Kazuki Kurimoto, Shinichiro Chuma, and Mitinori Saitou. "TDRD5 is required for retrotransposon silencing, chromatoid body assembly, and spermiogenesis in mice." Journal of Cell Biology 192, no. 5 (March 7, 2011): 781–95. http://dx.doi.org/10.1083/jcb.201009043.
Full textAbstract:
The Tudor domain–containing proteins (TDRDs) are an evolutionarily conserved family of proteins involved in germ cell development. We show here that in mice, TDRD5 is a novel component of the intermitochondrial cements (IMCs) and the chromatoid bodies (CBs), which are cytoplasmic ribonucleoprotein granules involved in RNA processing for spermatogenesis. Tdrd5-deficient males are sterile because of spermiogenic arrest at the round spermatid stage, with occasional failure in meiotic prophase. Without TDRD5, IMCs and CBs are disorganized, with mislocalization of their key components, including TDRD1/6/7/9 and MIWI/MILI/MIWI2. In addition, Tdrd5-deficient germ cells fail to repress LINE-1 retrotransposons with DNA-demethylated promoters. Cyclic adenosine monophosphate response element modulator (CREM) and TRF2, key transcription factors for spermiogenesis, are expressed in Tdrd5-deficient round spermatids, but their targets, including Prm1/Prm2/Tnp1, are severely down-regulated, which indicates the importance of IMC/CB-mediated regulation for postmeiotic gene expression. Strikingly, Tdrd5-deficient round spermatids injected into oocytes contribute to fertile offspring, demonstrating that acquisition of a functional haploid genome may be uncoupled from TDRD5 function.
20
Saito, M., M. Iwase, S. Maslan, N. Nozaki, M. Yamauchi, K. Handa, O. Takahashi, et al. "Expression of cementum-derived attachment protein in bovine tooth germ during cementogenesis." Bone 29, no. 3 (September 2001): 242–48. http://dx.doi.org/10.1016/s8756-3282(01)00573-7.
Full text
21
Ahmad, Ishtiaq, Bibi Aisha Sadiqa, and Rashid Khan. "The Impact of Corporate Governance Practices on the Firm Financial Performance of the Non-Financial Firms." Global Economics Review VI, no. I (March 30, 2021): 53–70. http://dx.doi.org/10.31703/ger.2021(vi-i).05.
Full textAbstract:
The study focuses on whether the Corporate Governance characteristics influence the firm performance of Non-Financial Firms in Pakistan. In this study, three types of industries like pharmaceutical, cement, and food were analyzed from the Pakistan Stock Exchange for the period of 2010 to 2019. The authors used the diagnostic test on data that argued that the model is better, like the fixed effect model or random-effect model for analysis. Multiple regression-based methodologies were developed to use a fixed-effect model for both dependent variables, Return on Assets and Tobin-Q variables, to discover the association between corporate governance and firm performance. It is concluded that board size, board education board experience, board nationality and board compensation have significant the ROA and board size, board experience, the board size, and board compensation shows significance with Tobin-Q.
22
Dahruji, Dahruji, Pipit Festy Wilianarti, and Totok Totok Hendarto. "Studi Pengolahan Limbah Usaha Mandiri Rumah Tangga dan Dampak Bagi Kesehatan di Wilayah Kenjeran, Surabaya." AKSIOLOGIYA : Jurnal Pengabdian Kepada Masyarakat 1, no. 1 (December 13, 2016): 36. http://dx.doi.org/10.30651/aks.v1i1.304.
Full textAbstract:
At this time the development of the industry in Indonesia is growing rapidly, there are currently large and small industries. An assortment of chemical industry, paper, textiles and cement. The small industry such as industry know. Many industries can generate positive and negative impacts. The positive impact of the industry, among others, the creation of jobs and the use of new technologies in various fields. The negative effects derived from industrial waste in the form of waste, liquid waste containing substances that are harmful to the surrounding community, so that the disposal of waste produces a toxic substance that causes the growth of bacteria that breed. With the indiscriminate disposal of waste fluids can cause problems for humans share, environment and water, can grow germs or other germs harmful to humans, would be susceptible to various diseases due to the influence of chemicals that pollute the river. Long-kelaman waste fluid change color to dark brown and smelly, and the stench is going to result in respiratory problems for people around. (Atinah fatal 2007).The research method using descriptive research method, with consecutive sampling technique to sample 21 people. Analysis using the percentage description. Results obtained 17 percent of skin disorders, diarrhea 2 percent, and laboratory values averaged COB try the 1 meter including pemcemaran being.With the existence of this study is expected an increase of knowledge in the management of household waste small industries.
23
Zhou, Tao, Jinhai Pan, Peiyao Wu, Ruijie Huang, Wei Du, Yachuan Zhou, Mian Wan, et al. "Dental Follicle Cells: Roles in Development and Beyond." Stem Cells International 2019 (September 15, 2019): 1–17. http://dx.doi.org/10.1155/2019/9159605.
Full textAbstract:
Dental follicle cells (DFCs) are a group of mesenchymal progenitor cells surrounding the tooth germ, responsible for cementum, periodontal ligament, and alveolar bone formation in tooth development. Cascades of signaling pathways and transcriptional factors in DFCs are involved in directing tooth eruption and tooth root morphogenesis. Substantial researches have been made to decipher multiple aspects of DFCs, including multilineage differentiation, senescence, and immunomodulatory ability. DFCs were proved to be multipotent progenitors with decent amplification, immunosuppressed and acquisition ability. They are able to differentiate into osteoblasts/cementoblasts, adipocytes, neuron-like cells, and so forth. The excellent properties of DFCs facilitated clinical application, as exemplified by bone tissue engineering, tooth root regeneration, and periodontium regeneration. Except for the oral and maxillofacial regeneration, DFCs were also expected to be applied in other tissues such as spinal cord defects (SCD), cardiomyocyte destruction. This article reviewed roles of DFCs in tooth development, their properties, and clinical application potentials, thus providing a novel guidance for tissue engineering.
24
Yang, Z.-H., X.-J. Zhang, N.-N. Dang, Z.-F. Ma, L. Xu, J.-J. Wu, Y.-J. Sun, Y.-Z. Duan, Z. Lin, and Y. Jin. "Apical tooth germ cell-conditioned medium enhances the differentiation of periodontal ligament stem cells into cementum/periodontal ligament-like tissues." Journal of Periodontal Research 44, no. 2 (April 2009): 199–210. http://dx.doi.org/10.1111/j.1600-0765.2008.01106.x.
Full text
25
Fujiwara, Naoki. "In vitro formation of cementum in mouse molar germs cultured in newly-developed organ culture system." Japanese Journal of Oral Biology 39, no. 2 (1997): 143–54. http://dx.doi.org/10.2330/joralbiosci1965.39.143.
Full text
26
Safiuddin, Md, Jeffrey S. West, and Khaled A. Soudki. "AIR CONTENT OF SELF-CONSOLIDATING CONCRETE AND ITS MORTAR PHASE INCLUDING RICE HUSK ASH / ORO KIEKIS SAVITANKIAME BETONE IR JO SKIEDINIO DALYJE SU RYŽIŲ LUKŠTŲ PELENAIS." Journal of Civil Engineering and Management 17, no. 3 (September 20, 2011): 319–29. http://dx.doi.org/10.3846/13923730.2011.589225.
Full textAbstract:
This paper presents the air content results of self-consolidating concrete (SCC) and its mortar phase including rice husk ash (RHA) as a supplementary cementing material. Moreover, this paper demonstrates a simple technique to determine the dosage of air-entraining admixture (AEA) required for the target air content in SCC. Different SCC mixtures were designed based on the water/binder (W/B) ratios of 0.30–0.50 and design air content of 4–8%. RHA was incorporated in the concretes substituting 0–30% of cement by weight. The mortars were formulated from the mixture proportions of the corresponding parent concretes and tested to determine the air content at various AEA dosages. The effects of W/B ratio and RHA content on the air content of both mortar and concrete were observed. The effect of mortar volume on the air content of concrete was also noticed. Besides, the AEA dosages required for the target air contents of concrete were estimated based on the equivalent mortar air contents. Later the air-entrained SCC mixtures were produced using AEA and tested for the air content. Test results exhibit that the air contents of both mortar and concrete were significantly influenced by the W/B ratio and RHA content. The concrete air content was also greatly influenced by its mortar volume. The AEA dosage increased with lower W/B ratio, higher RHA content, and greater mortar volume for the target air contents. In addition, the actual AEA dosages were consistent with the estimated AEA dosages of the concretes. An excellent correlation was observed between the actual and estimated AEA dosages. The strong correlation suggests that the AEA dosage needed for a target air content in concrete can be determined based on the equivalent air content of its mortar phase. Santrauka Straipsnyje pateikiami oro kiekio nustatymo savitankiame betone (SCC) ir jo skiedinio dalyje su ryžių lukštų pelenais (RHA), naudojamais kaip papildoma cementavimo medžiaga, rezultatai. Be to, pateikiamas paprastas būdas parinkti orą įsiurbiančio priedo (AEA) dozę, siekiant gauti reikalingą oro kiekį SCC. Suprojektuoti skirtingi SCC mišiniai su skirtingu vandens ir rišiklio (W/B) 0,30–0,50 santykiu ir numatytu 4–8 proc. oro kiekiu, RHA buvo dedamas į betoną pakeičiant 0–30 proc. cemento pagal masę. Skiediniai buvo formuojami pagal jiems artimo betono sudėtis ir oro kiekis juose bandomas su skirtingomis AEA dozėmis. Nustatyta W/B santykio ir RHA kiekio įtaka oro kiekiui tiek skiedinyje, tiek betone bei skiedinio tūrio įtaka oro kiekiui betone. Be to, AEA dozės, reikalingos numatytam oro kiekiui betone pasiekti, nustatytos pagal ekvivalentinį oro kiekį skiedinyje. Vėliau SCC mišiniai su orą įsiurbiančiu priedu buvo pagaminti naudojant AEA ir išbandytas juose esantis oro kiekis. Tyrimų rezultatai rodo, kad tiek skiedinį, tiek betono oro kiekį labai veikia W/B santykis ir RHA kiekis. Oro kiekį betone taip pat smarkiai veikia skiedinio tūris. Mažėjant W/B santykiui, didėjant RHA kiekiui ir skiedinio tūriui AEA dozė turi būti didinama norimam oro kiekiui pasiekti. Taip pat faktinės AEA dozės atitiko suskaičiuotas betonų AEA dozes. Tarp faktinių ir suskaičiuotų AEA dozių gauta labai gera koreliacija. Ji rodo, kad AEA dozę, reikalingą numatytam oro kiekiui betone pasiekti, galima skaičiuoti pagal ekvivalentinį oro kiekį šio betono skiedinio dalyje.
27
Antonovics, Janis, and Michael E. Hood. "Linnaeus, smut disease and living contagion." Archives of Natural History 45, no. 2 (October 2018): 213–32. http://dx.doi.org/10.3366/anh.2018.0515.
Full textAbstract:
This paper examines the rise and fall of Carl Linnaeus's ideas on living contagion, focusing on his work with plant smut diseases. Early in his career, Linnaeus named a plant altered by anther-smut disease as a separate species, but then, probably realizing it was a diseased specimen, demoted it to a variety. He later drew direct parallels between minute insects attacking plants and infectious diseases in humans, but did not yet draw an analogy to smut diseases. After Otto von Münchhausen had sent Linnaeus the first instalment of his book Der Hausvater (1764) , Linnaeus realized smuts were contagious. He carried out his own investigations that appeared to confirm Münchhausen's conclusion that smut spores germinated to produce living and mobile animalcules. This cemented Linnaeus's view that animalcules caused contagion in human diseases, a view which he expressed forcefully, urging further studies. However, his results were questioned and discounted by others, especially John Ellis. An analysis of correspondence between Linnaeus and other microscopists shows that it is likely Linnaeus did actually see “animalcules” emerging from cereal grains. He was unaware that smut-like symptoms in wheat could also be caused by seed-gall nematodes in the genus Anguina. Linnaeus himself came to doubt the connection between fungi and contagion, and did not pursue these studies further. The presumption that Linnaeus was fanciful in his observations of animalcules may partly explain why his views had only a tangential impact on the germ-theory of disease, and why his insights remain unappreciated to this day.
28
Dias, Letícia Andreolli, Daiana Cristina Metz Arnold, Fernanda Boll Birck, and Adriana Teresinha Silva. "CONCRETO REFORÇADO COM RESÍDUO TÊXTIL ORIUNDO DA PRODUÇÃO DE PNEU." Revista Tecnologia e Tendências 10, no. 1 (March 12, 2019): 107. http://dx.doi.org/10.25112/rtt.v10i1.1509.
Full textAbstract:
A construção civil está em busca constante de materiais alternativos que supram as solicitações exigidas nas estruturas das edificações. Nesse contexto, as fibras têxteis residuais podem ser uma alternativa para as matrizes cimentícias como reforço estrutural contra a fissuração localizada, uma vez que o concreto armado é incapaz de absorvê-las. Por sua vez, a indústria de pneus gera grandes quantidades de resíduos têxteis que são destinados aos aterros sanitários, causando graves impactos ao meio ambiente. Nesse sentido, esta pesquisa buscou avaliar a viabilidade da incorporação de resíduo têxtil em forma de fibras, em concretos, visando aumentar a capacidade de deformação e o controle da fissuração. Para tanto, confeccionou-se um traço 1:2,21:3,21 (cimento:areia:brita) e incorporou-se as fibras nos teores de 2,7 e 3,6 Kg/m³ sobre o volume do concreto. Para caracterização das fibras foi verificada a geometria, o comprimento, o fator de forma e o diâmetro médio. No estado fresco do concreto, analisou-se a influência das fibras quanto à trabalhabilidade e a massa específica. Após, no estado endurecido, avaliou-se a densidade de massa, a resistência à compressão, a resistência à tração por compressão diametral e a resistência ao impacto. Além disso, verificou-se a forma de aderência e de ruptura da fibra na matriz cimentícia através do microscópio óptico. Deste modo, os resultados apontam viabilidade da incorporação de fibra têxtil em matrizes cimentícias, visto que estas diminuiram a fissuração localizada e auxiliaram na absorção de impactos no concreto, porém por se tratar de um assunto inovador, deve-se realizar mais estudos para que o compósito possa ser efetivamente utilizado na construção civil.Palavras-chave: Concreto. Resíduo têxtil. Fissuras localizadas.ABSTRACTThe civil construction is constantly seeking for alternative materials that meet the requirements of the buildings structures. In this context, residual textile fibers may be an option for cement matrix as a structural reinforcement against the localized cracking, considering that the reinforced concrete is unable to absorb them. With regard, the tire industry generates substantial amounts of textile waste that is destined for landfill sites, causing massive impacts to the environment. In this sense, this research sought to evaluate the feasibility of the incorporation of textile waste in the fibers form, in concrete, aiming to increase the deformation capacity and the control of cracking. In order to do so, the trace 1: 2,21: 3,21 (cement: sand: gravel) was made and the fibers were incorporated in the contents of 2.7 and 3.6 kg / m³ on the volume of the concrete. For characterization of the fibers, the geometry, length, shape factor and average diameter were verified. In the fresh state of the concrete, it was analyzed the influence of the fibers on the workability and the specific mass. Later, in the hardened state, the mass density, compressive strength, diametral compression tensile strength and impact strength were evaluated. In addition to that, the adhesion and rupture of the fiber in the cementitious matrix ware checked through the optical microscope. Thus, the results indicate the feasibility of the incorporation of textile fiber in cementitious matrixes, since these reduced the localized cracking and helped in the absorption of impacts in the concrete; however, since it is an innovative subject, more studies may be done so that the composite can be used in civil construction.Keywords: Concrete. Textile residue. Localized cracks.
29
Kurtinaitis, Jaunius, Narūnas Porvaneckas, Manvylius Kocius, and Rimvaldas Broga. "Pirminis mišraus ir mechaninio tvirtinimo klubo sąnario endoprotezavimas. Vėlyvieji 5–10 metų rezultatai." Lietuvos chirurgija 1, no. 2 (January 1, 2003): 0. http://dx.doi.org/10.15388/lietchirur.2003.2.2428.
Full textAbstract:
Jaunius Kurtinaitis, Narūnas Porvaneckas, Manvylius Kocius, Rimvaldas BrogaVilniaus universiteto Ortopedijos-traumatologijos ir plastinės chirurgijos klinikaVilniaus greitosios pagalbos universitetinė ligoninėŠiltnamių g. 29, LT-2043 Vilnius.El paštas: narunas.porvaneckas@mf.vu.lt Įvadas / tikslas Dažniausia endoprotezuoto klubo sąnario problema – gūžduobinio komponento intarpo susidėvėjimas arba jo išklibimas. Vieni autoriai pripažįsta cementinio, kiti – mechaninio tvirtinimo metodiką. Abiem atvejais vėlyvieji rezultatai yra panašūs. Kiekviena metodika tam tikru endoprotezuoto sąnario laikotarpiu išryškina jai būdingas problemas. Mūsų darbo tikslas – įvertinti vėlyvuosius mišraus ir mechaninio tvirtinimo klubo sąnario pirminio endoprotezavimo rezultatus po operacijos praėjus 5–10 metų. Ligoniai ir tyrimo metodai 1993–1998 metais VGPUL Ortopedijos, traumatologijos ir plastinės chirurgijos klinikoje buvo operuoti 36 ligoniai, kuriems buvo atliktos 39 pirminės klubo sąnario endoprotezavimo operacijos naudojant Bi-Metric mechaninio ir mišraus tvirtinimo endoprotezus (EP). Į tyrimą įtraukti 27 ligoniai (29 endoprotezai – 18 mechaninio ir 11 mišraus tvirtinimo), 2002–2003 metais atvykę pasitikrinti. Vidutinis pacientų su mechaninio tvirtinimo protezais stebėjimo laikas (mediana) siekė 7 metus, o su mišraus tvirtinimo – 5 metus. Visiems atvykusiems pacientams buvo atliktos ir įvertintos priekinės abiejų klubo sąnarių rentgenogramos, nustatyta klubo sąnario funkcija pagal Harris Hip Score skalę. Rezultatai 2002–2003 m. ištirta 29 (80%) iš 36 ligonių, operuotų 1993–1998 m. Rentgenologinių tyrimų rezultatai parodė, kad aplink mechaninio tvirtinimo komponentus linijinės demarkacijos nėra. Visi komponentai buvo stabilūs. Kaulas įaugo į mechaninio tvirtinimo komponentus 100% atvejų. Įvertintos osteolizinio proceso aplink gūžduobinį ir šlaunikaulinį komponentus ypatybės, polietileninio intarpo susidėvėjimo prognozės veiksniai atsižvelgiant į endoprotezo tipą, ektopinė osifikacijos įtaka vėlyviesiems klubo sąnario funkciniams rezultatams. Išvados Įvertinti pagal HHS skalę mechaninio ar mišraus tvirtinimo endoprotezavimo rezultatai po 5–10 metų yra geri ir labai geri. Lyginant rentgenologinius pokyčius aplink gūžduobinį ir šlaunikaulinį komponentus matyti, kad pokyčiai aplink mechaninio ir cementinio tvirtinimo šlaunikaulinius komponentus yra gerokai mažesni negu aplink gūžduobinius. Statistiškai patikimo skirtumo tarp polietileno intarpo susidėvėjimo ir panaudoto endoprotezo tipo nebuvo. Gūžduobinio ir šlaunikaulinio komponentų osteoliziniai procesai yra besimptomiai. Šie procesai ir 76% atvejų nustatyta ektopinė osifikacija neturi įtakos geriems ir labai geriems vėlyviesiems operuoto klubo sąnario funkciniams rezultatams. Prasminiai žodžiai: pirminis klubo sąnario endoprotezavimas, mechaninio tvirtinimo endoprotezai, mišraus tvirtinimo endoprotezai. Primary hybrid and cementless total hip arthroplasty. 5 to 10 year follow-up results Jaunius Kurtinaitis, Narūnas Porvaneckas, Manvylius Kocius, Rimvaldas Broga Background / objective The major issue of hip arthroplasty is the wearout or loosening of the acetabular component. Some authors prefer cemented and others cementless hip arthroplasty. In both cases the outcome is similiar. Each method in different periods has its own problems. The aim of the study was to evaluate the late outcome of primary cementless and hybrid total hip replacement 5–10 years after operation. Patients and methods There were 36 patients operated on and 39 operations of primary total hip arthroplasty performed using Bi-Metric hybrid and cementless endoprostheses in 1993–1998. The study included 27 patients (29 endoprostheses) who responded to invitation in 2002–2003. Anteroposterior radiographs of the hip joints were performed for the patients and the functional outcome was evaluated using the Harris Hip score scale. Results During 2002–2003 investigation was carried out on 29 (74%) of 36 hip arthroplasties performed in 1993–1998. Analysis of the data showed no radiolucency signs around the cementless fixation components. All of the components were stable. Bone ingrowth into the cementless prosthesis components was observed in 100% of cases. The osteolysis zones around the acetabular and femoral components were related to the wearout of polythene insertion, but it was asymptomatic. There was no reliable difference between polythene insertion wearout and the type of prosthesis. Ectopic ossification was observed in 76% of cases. Conclusions The study based on HHS score revealed good and very good results 5–10 years after operation. They were comparable with the findings in similar studies. Rentgenological features indicated that there were less alterations around the femoral component compared to the acetabular. Osteolysis around the femoral and acetabular components was asymptomatic and ectopic ossification found in 76% of cases did not interfere with the good functional results. Keywords: primary total hip arthroplasty, cementless prostheses, hybrid prostheses
30
Liu, Wan-Sheng, Chen Lu, and Bhavesh V. Mistry. "Subcellular localization of the mouse PRAMEL1 and PRAMEX1 reveals multifaceted roles in the nucleus and cytoplasm of germ cells during spermatogenesis." Cell & Bioscience 11, no. 1 (June 1, 2021). http://dx.doi.org/10.1186/s13578-021-00612-6.
Full textAbstract:
Abstract Background Preferentially expressed antigen in melanoma (PRAME) is a cancer/testis antigen (CTA) that is predominantly expressed in normal gametogenic tissues and a variety of tumors. Members of the PRAME gene family encode leucine-rich repeat (LRR) proteins that provide a versatile structural framework for the formation of protein–protein interactions. As a nuclear receptor transcriptional regulator, PRAME has been extensively studied in cancer biology and is believed to play a role in cancer cell proliferation by suppressing retinoic acid (RA) signaling. The role of the PRAME gene family in germline development and spermatogenesis has been recently confirmed by a gene knockout approach. To further understand how PRAME proteins are involved in germ cell development at a subcellular level, we have conducted a systematic immunogold electron microscopy (IEM) analysis on testis sections of adult mice with gene-specific antibodies from two members of the mouse Prame gene family: Pramel1 and Pramex1. Pramel1 is autosomal, while Pramex1 is X-linked, both genes are exclusively expressed in the testis. Results Our IEM data revealed that both PRAMEL1 and PRAMEX1 proteins were localized in various cell organelles in different development stages of spermatogenic cells, including the nucleus, rER, Golgi, mitochondria, germ granules [intermitochondrial cement (IMC) and chromatoid body (CB)], centrioles, manchette, and flagellum. Unlike other germ cell-specific makers, such as DDX4, whose proteins are evenly distributed in the expressed-organelle(s), both PRAMEL1 and PRAMEX1 proteins tend to aggregate together to form clusters of protein complexes. These complexes were highly enriched in the nucleus and cytoplasm (especially in germ granules) of spermatocytes and spermatids. Furthermore, dynamic distribution of the PRAMEL1 protein complexes were observed in the microtubule-based organelles, such as acroplaxome, manchette, and flagellum, as well as in the nuclear envelope and nuclear pore. Dual staining with PRAMEL1 and KIF17B antibodies further revealed that the PRAMEL1 and KIF17B proteins were co-localized in germ granules. Conclusion Our IEM data suggest that the PRAMEL1 and PRAMEX1 proteins are not only involved in transcriptional regulation in the nucleus, but may also participate in nucleocytoplasmic transport, and in the formation and function of germ cell-specific organelles during spermatogenesis.
31
Ammar, A. M., E. A. El Zayyat, A. E. Khayyal, and N. A. Elleboudy. "Population dynamics of some domestic mites in laboratory culture." Journal of Basic and Applied Zoology 82, no. 1 (March 2, 2021). http://dx.doi.org/10.1186/s41936-021-00213-2.
Full textAbstract:
Abstract Background Domestic mites are a primary source of allergens indoors all over the world. Extracts of mite allergens are important for diagnosing and treating mites allergy. The effective cultivation processes play a critical role in the final composition of mites' allergen extracts. In order to produce large amounts of antigens of domestic mites, culture techniques of domestic mites were studied. Domestic mites were isolated from collected dust samples and cultured on a fine-ground mixture of dust and sawdust (2:1). While dry yeast, wheat germ, and cornflour were used at a ratio of 2:1:1 for nutrition. Food was placed over a few centimeters of cement base that had been placed on the bottom of the rearing containers. The population growth rates were determined. Results Successful methods for mite cultures utilize high protein and carbohydrate foods, an average temperature of (25 ± 2) °C and relative humidity (80 ± 5) %. During a 14-week observation period of mites' culture, the peak in number was obtained after 8 weeks of the culture in Tyrophagus, 10 weeks in Dermatophagoides, and 12 weeks in Blomia; thereafter, the number decreased. Significant differences were found in the growth rates of Dermatophagoides (P = 0.02) and Blomia (P = 0.03) in between pure and mixed cultures. Conclusions The modifications made to the mixture of diet used for cultivation showed a high yield of domestic mites. Also, the cement layer also is an excellent way to make space for egg laying and prevent mites from escaping.
32
Techa‐ungkul, Chutima, and Rangsima Sakoolnamarka. "The effect of dentin age on the microshear bond strength and microleakage of glass‐ionomer cements." Gerodontology, December 22, 2020. http://dx.doi.org/10.1111/ger.12520.
Full text
33
Costa, Larissa Souza. "Comparação entre os antígenos do extrato sonicado de Porphyromonas gingivalis ATCC3277 e a proteína recombinante HmuY na indução da produção de HSP60 por CMSP de indivíduos com periodontite." Anais dos Seminários de Iniciação Científica, no. 22 (February 4, 2019). http://dx.doi.org/10.13102/semic.v0i22.3859.
Full textAbstract:
As doenças periodontais, são doenças infecciosas geradoras de condições inflamatórias, que resultam na destruição dos tecidos de sustentação dos dentes (osso, ligamento periodontal e cemento). Esse processo ocorre em decorrência do desequilíbrio entre bactérias e fatores do hospedeiro, resultado de alterações disbióticas que ocorrem no biofilme subgengival (HAJJISHENGALIS e LAMONT, 2014). Muitos estudos apontam para um papel patogênico de Porphyromonas gingivalis, colonizador secundário da cavidade oral, cuja presença requer a criação de condições ambientais por microrganismos antecedentes. É considerado um patógeno-chave na disbiose periodontal (HAJISHENGALIS, 2014), produzindo uma ampla gama de potentes fatores de virulência envolvidos na colonização e destruição tecidual, bem como na modulação da resposta do hospedeiro (ARUNI et al., 2011). Dentre os fatores de virulência destaca-se ainda a lipoproteína HmuY, uma proteína captadora de ferro que induz o aumento nos níveis de IL-10, iL-6,IgG e IgG1 anti-HmuY e inibe a produção de IL-8 por células do sistema imune do hospedeiro (TRINDADE et al, 2012; TRINDADE et al, 2013).Em resposta as agressões provocadas pelos microrganismos durante a infecção, é sabido que as células possuem mecanismos de proteção bastante eficazes, como as proteínas de choque térmico (HSP). O estresse celular induzido por uma gama de condições, tais como, exposição a altas temperaturas, estresse radioativo, irradiação ultravioleta e infecção viral (POCLEY et AL, 1999), assim como por perturbações orgânicas incluindo hipóxia, processos inflamatórios, exposição a toxinas e danos ao DNA, geram diretamente nestas células uma resposta na tentativa de preservar sua homeostasia auxiliadas pelas proteínas de choque térmico.Recentes estudos têm mostrado evidências que as HSP, especialmente HSP60, possuem propriedades que permitem seu uso na geração de respostas imune específicas contra câncer e agentes infecciosos (PARSELL & LINDQUIST, 1993; LI, et al., 2002; SEGAL et al., 2006; CAPELLO et al., 2008; JEGO et al. 2013).Assim, o objetivo do presente estudo é comparar os níveis de HSP60 em culturas de CMSP estimuladas com o extrato sonicado e com a proteína HmuY de Porphyromonas gingivalis.
34
Bottechia, Juliana Alves de Araújo, Márcia Barbosa Gobira, and Ana Lucia Martins Sousa. "Licões de Mariana e a contaminação do Rio Doce / Lessons from Mariana and the contamination of the Doce River." Revista de Direito da Administração Pública 1, no. 1 (February 10, 2017). http://dx.doi.org/10.47096/redap.v1i1.91.
Full textAbstract:
<p><span>No Brasil, diferente de outros paÃses, o solo é propriedade da federação, logo para regulamentar e fiscalizar as atividades de retirada de qualquer minério no nosso paÃs, foi criado o Departamento Nacional de Produção Mineral (DNPM), ligado ao Ministério responsável. Como atividade que gera lucros, a mineração é tributada: a Compensação Financeira por Exploração Mineral (CFEM). Do valor da compensação recolhido no Estado de Goiás por exemplo, é redistribuÃdo desta forma: 23% para o Estado e Distrito Federal; 65% aos municÃpios; 2% ao Fundo Nacional de Desenvolvimento CientÃfico e Tecnológico; e 10% ao DNPM, e destes, 2% devem ser destinados para a proteção ambiental. A maior produção mineral em Goiás (2011) foi de NÃquel, seguido de Cobre, Ouro, Amianto, Fosfato, Cobalto e Nióbio. A maior produção do MunicÃpio de Formosa no ano de 2012 foi de Argila vermelha, seguida de Brita, Areia, Calcário agrÃcola, Argila para cimento, Cascalho e Ãgua mineral. Para obter tal produção, as empresas mineradoras precisam obter licenças o que envolve itens de segurança obrigatórios como a construção de barragens para conter rejeitos, mas o recente acidente em Mariana/MG envolveu muitas vidas, inclusive a do Rio Doce, por falta da proteção ambiental: da tragédia, fica a lição.  </span></p><p><span><br /></span></p><p>In Brazil, unlike other countries, the soil is owned by the federation, so to regulate and supervise the activities of withdrawal of any ore in our country, the National Department of Mineral Production (DNPM) was created, linked to the responsible Ministry. As an activity that generates profits, the mining is taxed: the Financial Compensation for Mineral Exploration (CFEM). Of the value of the compensation collected in the State of Goiás for example, it is redistributed in this way: 23% for the State and Federal District; 65% to municipalities; 2% to the National Fund for Scientific and Technological Development; And 10% to DNPM, of which 2% should be earmarked for environmental protection. The largest mineral production in Goiás (2011) was Nickel, followed by Copper, Gold, Asbestos, Phosphate, Cobalt and Niobium. The largest production of the Municipality of Formosa in the year 2012 was of Red Clay, followed by Brita, Sand, Agricultural Limestone, Clay for Cement, Gravel and Mineral Water. To obtain such production, mining companies must obtain permits, which involve mandatory safety items such as the construction of dams to contain tailings, but the recent Mariana/MG accident involved many lives, including Rio Doce, due to the lack of environmental protection: From tragedy, the lesson remains.</p><p><span><br /></span></p>