Academic literature on the topic 'Centrifugation technique'

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Journal articles on the topic "Centrifugation technique"

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Maharani, Eva Ayu, and Dewi Astuti. "Comparison of Single Centrifugation, Double Centrifugation and Turn down-Turn up Techniques for Platelet-Rich Plasma Quality." Althea Medical Journal 9, no. 3 (2022): 180–84. http://dx.doi.org/10.15850/amj.v9n3.2628.

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Background: Platelet-rich plasma (PRP) is a new concept used in medical world, especially for wound healing. The main process that affects the PRP quality is the centrifugation process. This study aimed to assess the PRP separation process and determine the best technique of various centrifugation process.Methods: This experimental study used acid citrate dextrose (ACD) blood taken from 11 healthy respondents and compared three-techniques including the single centrifugation (SC), the double centrifugation (DC), and the double centrifugation turn down - turn up (DC-TDTU) techniques. The quality
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Walker, Randall C. "Lysis-Centrifugation Blood Culture Technique." Archives of Internal Medicine 146, no. 12 (1986): 2341. http://dx.doi.org/10.1001/archinte.1986.00360240055010.

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Novia, Diana, and Binarwan Halim. "#302 : Shorter Centrifugation Time Leads to Better Sperm Quality in Swim-Up Processing Technique." Fertility & Reproduction 05, no. 04 (2023): 700. http://dx.doi.org/10.1142/s2661318223744120.

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Background and Aims: Centrifugation is one of the many factors that cause sperm DNA damage in IVF semen preparation. This can occur due to the formation of reactive oxygen species (ROS) that are uncontrollable, so that the quality of sperm DNA can be damaged. This study aims to compare sperm quality results with swim-up technique under centrifugation times of 5 and 10 minutes. Method: This study involved 50 patients at the Halim Fertility Center clinic from June 2020 to October 2020. The samples obtained were processed using the swim-up method. Samples were divided into 3 groups, namely contro
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Fallatah, Majed Hashem, Aaied Falah Al Otaibi, Randa Rajallah Aljohani, Waad Jamal Ghabban, and Alaa Ali Alshehri. "Evaluation of Centrifugation Technique and the Effect of Centrifugation Condition on the Laboratory Samples." JOURNAL OF HEALTHCARE SCIENCES 04, no. 12 (2024): 1003–9. https://doi.org/10.52533/johs.2024.41248.

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Centrifugation is a critical laboratory technique used to separate components within a mixture based on density differences. The efficiency and reliability of this process depend on the precise control of variables such as speed, time, temperature, and rotor design. Improper centrifugation settings can compromise sample integrity, leading to analytical inaccuracies. High-speed centrifugation, while effective for rapid separation, often imposes mechanical stress on samples, resulting in hemolysis or cellular damage. Time duration is equally significant, as insufficient centrifugation can cause
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Taglioretti, V., N. Sardella, and M. Fugassa. "Effectiveness of coproscopic concentration techniques." Helminthologia 51, no. 3 (2014): 210–14. http://dx.doi.org/10.2478/s11687-014-0231-x.

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AbstractThe aim of the present study was to compare the effectiveness of the concentration techniques of flotation-centrifugation with zinc chloride (FZn) (δ = 1.45) with the spontaneous sedimentation (SSed) and the sucrose flotation-centrifugation (FSuc) (δ = 1.2) to recuperate gastrointestinal parasites from camelid fecal samples. The technique with more positive results for the detection of Nematodirus sp., Trichuris sp., strongyle-type eggs and Eimeria macusaniensis oocysts was the FZn. For Trichuris sp. and Eimeria macusaniensis, the higher coverglass counts were detected by FZn procedure
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Lebl, Michal. "Centrifugation Based Automated Synthesis Technologies." JALA: Journal of the Association for Laboratory Automation 8, no. 3 (2003): 30–35. http://dx.doi.org/10.1016/s1535-5535-04-00267-9.

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Centrifugation is a powerful method for solid-liquid separation. It can be applied in numerous ways to simplify solid phase synthetic procedures. At the same time, centrifugation is the only totally parallel technique which can be scaled up for processing volume or number of simultaneously run reactions, without the limitation of overpressure or vacuum-driven filtration-based systems. We have developed synthesizers based on the power of centrifugation — peptide and small organic molecule synthesizers utilizing cotton as the synthetic substrate and inclusion volume chemistry, synthesizers for a
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Matas, C., J. Gadea, and G. Decuadro-Hansen. "89 EVALUATION OF A CUSHIONED CENTRIFUGATION TECHNIQUE FOR PROCESSING BOAR SEMEN FOR FREEZING." Reproduction, Fertility and Development 17, no. 2 (2005): 195. http://dx.doi.org/10.1071/rdv17n2ab89.

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Boar semen freezing procedures include the use of centrifugation to concentrate sperm and remove seminal plasma prior to dilution in freezing extender. The centrifugation techniques employed have necessarily been a compromise between the need to recover as many spermatozoa as possible after centrifugation and the damage caused by pelleting the sperm. The use of an inert, dense, and isotonic solution as a cushion in the bottom of the tube leads to the use of higher-speed centrifugation to ensure maximum sperm recovery. However, it is necessary to know the viability and functionality of the samp
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Singhal, Ankita, Sheetal Goenka, and Dimple Kasana. "Revolutionizing Sterile Sample Culturing: Cost-Effective Centrifugation Techniques for Research in Resource- Constrained Settings." European Journal of Medical and Health Research 1, no. 1 (2023): 30–33. http://dx.doi.org/10.59324/ejmhr.2023.1(1).05.

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Introduction: The yield (culture positive rate) of sterile body fluids in patients was found to be less than 50%. It has been demonstrated that centrifuging body fluid before culturing increases yield. Using concentrated bacterial samples to enhance culture growth is a more effective alternative. We sought to determine whether sterile fluid culture yield was increased by centrifugation. Methods: Patients whose body fluids were received in sufficient quantity in the microbiology lab were enrolled. Sterile body fluids culture was carried out after centrifugation and by routine conventional inocu
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Singhal, Ankita, Sheetal Goenka, and Dimple Kasana. "Revolutionizing Sterile Sample Culturing: Cost-Effective Centrifugation Techniques for Research in Resource- Constrained Settings." European Journal of Medical and Health Research 1, no. 1 (2023): 30–33. https://doi.org/10.59324/ejmhr.2023.1(1).05.

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Introduction: The yield (culture positive rate) of sterile body fluids in patients was found to be less than 50%. It has been demonstrated that centrifuging body fluid before culturing increases yield. Using concentrated bacterial samples to enhance culture growth is a more effective alternative. We sought to determine whether sterile fluid culture yield was increased by centrifugation. Methods: Patients whose body fluids were received in sufficient quantity in the microbiology lab were enrolled. Sterile body fluids culture was carried out after centrifugation and by routine conventional inocu
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Santana, Tamara, Jorge Moreno, Guillermo Petzold, Roberto Santana, and Guido Sáez-Trautmann. "Evaluation of the Temperature and Time in Centrifugation-Assisted Freeze Concentration." Applied Sciences 10, no. 24 (2020): 9130. http://dx.doi.org/10.3390/app10249130.

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Centrifugation is a technique applied to assist in the freeze concentration of fruit juices and solutions. The aim of this work was to study the influence of the time–temperature parameters on the centrifugation process as a technique applied to assist in the first cycle of the freeze concentration of blueberry juice. A completely randomized 4 × 3 factorial design was performed using temperature and time as the factors, and the response variables included the percentage of concentrate, efficiency and solutes recovered. The results were evaluated using multiple linear regression, random forest
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Dissertations / Theses on the topic "Centrifugation technique"

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Lopez, Isabelle. "« Développement d'un système universel de coulée d'alliages dentaires par centrifugation axiale à très haute vitesse sous vide secondaire »." Thesis, Reims, 2013. http://www.theses.fr/2013REIMO201.

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La fabrication de prothèses dentaires à base de titane a connu un réel essor ces dernières années. Cependant, la production de ces produits reste assez coûteuse, et le choix de producteurs est encore restreint. Les partenaires du projet de recherche Européen Den-ticast® ont identifié un fort potentiel pour la création d'une machine innovante, capable de produire ces pièces à un coût réduit et à un niveau de qualité élevé. Le projet est axé sur le domaine de la production de pièces de métal pour l'usage dentaire, avec comme objectif, le développement d'un nouveau système polyvalent de coulée de
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Robinson, Helen Jane. "Development of centrifugation as a technique for measuring cell-substrate adhesion and its use in the comparison of potential, titanium-based, implant materials." Thesis, University of Cambridge, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.608430.

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Lutumba-Tshindele, Pascal. "Contribution à la prise des décisions stratégiques dans le contrôle de la trypanosomiase humaine africaine." Doctoral thesis, Universite Libre de Bruxelles, 2005. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/210979.

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RESUME<p>La Trypanosomiase Humain Africaine (THA) demeure un problème de santé publique pour plusieurs pays en Afrique subsaharienne. Le contrôle de la THA est basé essentiellement sur la stratégie de dépistage actif suivi du traitement des personnes infectées. Le dépistage actif est réalisé par des unités mobiles spécialisées, bien que les services de santé fixes jouent un rôle important en détectant « passivement » des cas. Le dépistage reposait jadis sur la palpation ganglionnaire mais, depuis le développement du test d’agglutination sur carte (CATT), trois possibilités se sont offertes aux
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Books on the topic "Centrifugation technique"

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D, Rickwood, ed. Preparative centrifugation: A practical approach. IRL Press at Oxford University Press, 1992.

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K, Brownfield Isabelle, and Geological Survey (U.S.), eds. Improved density gradient separation techniques using sodium polytungstate and a comparison to the use of other heavy liquids. U.S. Dept. of the Interior, U.S. Geological Survey, 1993.

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K, Brownfield Isabelle, and Geological Survey (U.S.), eds. Improved density gradient separation techniques using sodium polytungstate and a comparison to the use of other heavy liquids. U.S. Dept. of the Interior, U.S. Geological Survey, 1993.

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1949-, Müller S. C., ed. Synthetic peptides as antigens. Elsevier, 1999.

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Graham, J., and D. Rickwood. Centrifugation. Springer Singapore Pte. Limited, 1999.

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A, Price C. Centrifugation in Density Gradients. Elsevier Science & Technology Books, 2014.

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Meisner, Lois Eilene. Rapid Determination of Collagen by Centrifugation Technique. Creative Media Partners, LLC, 2021.

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Birnie, G. D., and D. Rickwood. Centrifugal Separations in Molecular and Cell Biology. Elsevier Science & Technology Books, 2014.

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Naidoo, Shalinee. Centrifugation Techniques. Arcler Education Inc, 2017.

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Graham, J. M., and T. C. Ford. Introduction to Centrifugation (Introduction to Biotechniques). Coronet Books, 1991.

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Book chapters on the topic "Centrifugation technique"

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Bradecamp, Etta A. "Centrifugation of Semen: Standard Technique." In Equine Reproductive Procedures. John Wiley & Sons, Inc, 2014. http://dx.doi.org/10.1002/9781118904398.ch130.

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Bradecamp, Etta A. "Centrifugation of Semen: Cushion Technique." In Equine Reproductive Procedures. John Wiley & Sons, Inc, 2014. http://dx.doi.org/10.1002/9781118904398.ch131.

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Moskowitz, Illa L., Liya L. Regel, and William R. Wilcox. "Influence of Centrifugation on Furnace Temperature Distribution and Freezing Rate of InSb Using the Gradient Freeze Technique." In Processing by Centrifugation. Springer US, 2001. http://dx.doi.org/10.1007/978-1-4615-0687-4_33.

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Joosten, Matthieu H. A. J. "Isolation of Apoplastic Fluid from Leaf Tissue by the Vacuum Infiltration-Centrifugation Technique." In Plant Fungal Pathogens. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-501-5_38.

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Bradecamp, Etta A. "Centrifugation of Semen: Selection of Motile Sperm Using a Single Layer Colloid Technique." In Equine Reproductive Procedures. John Wiley & Sons, Inc, 2014. http://dx.doi.org/10.1002/9781118904398.ch132.

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Khasim, S. M., K. Thammasiri, S. Rama Rao, and M. Rahamtulla. "Centrifugation." In Plant Techniques. CRC Press, 2024. http://dx.doi.org/10.1201/9781003503682-7.

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Cazenave, J. P., J. N. Mulvihill, A. Sutter-Bay, C. Gachet, F. Toti, and A. Beretz. "A Centrifugation Technique for the Preparation of Suspensions of Non Activated Washed Human Platelets." In Test Procedures for the Blood Compatibility of Biomaterials. Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-1640-4_37.

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Coll, Hans, and Larry E. Oppenheimer. "Improved Techniques in Disc Centrifugation." In Particle Size Distribution. American Chemical Society, 1987. http://dx.doi.org/10.1021/bk-1987-0332.ch014.

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Kurien, Biji T. "Protein Blotting By Centrifugation." In Techniques in Life Science and Biomedicine for the Non-Expert. Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-70684-5_24.

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Shiba, Kiyotaka. "Particulars of Oral Cavity." In Extracellular Fine Particles. Springer Nature Singapore, 2025. https://doi.org/10.1007/978-981-97-7067-0_16.

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Abstract Oral fluids (OFs) contain a diverse array of extracellular vesicles (EVs) that hold promise as a source of diagnostic information. Developing EV-based diagnostics using OFs requires an understanding of the physicochemical properties and heterogeneity of these EVs. This review explores strategies for differentiating EVs in OFs, including differential centrifugation, density gradient centrifugation, and a novel method based on sedimentation patterns. These techniques have revealed distinct subpopulations of EVs in OFs, each associated with specific biological functions and potential diagnostic utility. However, the complexity of EVs in OFs presents challenges, and a comprehensive understanding of their biogenesis and composition is still emerging. Future research should focus on refining EV isolation methods and exploring the diagnostic potential of both EV and non-EV particles in OFs.
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Conference papers on the topic "Centrifugation technique"

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Nichols, W. L., S. E. Kaese, D. A. Gastineau, L. A. Otteman, and E. J. W. Bowie. "BERNARD-SOULIER SYNDROME: WHOLE BLOOD DIAGNOSTIC ASSAYS OF PLATELETS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644561.

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Diagnosis of Bernard-Soulier syndrome (BSS) is complicated by the difficulty of separating the giant platelets from other blood cells to pursue analyses of platelet function and structure. We report on the utility of three whole blood assay techniques for diagnosis of a patient with BSS. To our knowledge, these three techniques have not been simultaneously applied or compared for efficacy in laboratory diagnosis of BSS. (1) Whole blood platelet aggregation responses, studied with an electrical impedence aggregometer, were equivalent to those more laboriously obtained using platelet-rich plasma
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Hashimoto, Shigehiro. "Hysteresis Effect of Tangential Force Field With Centrifuge on Myoblast: Cultured on Striped Pattern of Micro Ridge for Direction Control." In ASME 2021 Fluids Engineering Division Summer Meeting. American Society of Mechanical Engineers, 2021. http://dx.doi.org/10.1115/fedsm2021-65639.

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Abstract Hysteresis effects of the direction of mechanical stimulation on the cell behavior have been examined in vitro. A micro ridge pattern was made on the surface of the scaffold to align the directions of the cells being stimulated. The stripe pattern (0.7 μm heigh, 3 μm wide, and 3 μm interval) was created by the photolithography technique. Three regions, which have the uniform value of the angle between the longitudinal direction of the ridge and the direction of the tangential force, were set: 0, 45, and 90 degrees in each region. Myoblasts (C2C12: mouse myoblast cell line) were used i
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khan, Shoyeb, Probir Das, Mohammed Abdul Quadir, Mahmoud Thaher, and Hareb Al Jabri. "Pretreatment of Cyanobacterial Chroococcidiopsis: Biomass prior to Hydrothermal Liquefaction for Enhanced Hydrocarbon Yield and Energy Recovery." In Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2020. http://dx.doi.org/10.29117/quarfe.2020.0024.

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Chroococcidiopsis sp. was grown in 200 L open raceway pond. Biomass density and average biomass productivity were 0.41 g/L and 16.1 g/m2/d. Chroococcidiopsis biomass was harvested by self-settling. Self settled biomass was further subjected to centrifugation to obtain a biomass paste with 25-30% solid content. Centrifuged biomass was dried at 80 °C overnight and used as a feedstock for pretreatment step. Biomass was pretreated in water at 105 °C for 15 minutes. A slurry containing 15 wt% pretreated and untreated biomass (control) in deionized water was prepared and subjected to hydrothermal li
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Elias, M. K., and C. Th Smit Sibinga. "PRODUCTION OF PLATELETS FOR TRANSFUSION - CURRENT METHODS AND PROBLEMS TO BE SOLVED." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643998.

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Initially, whole blood or platelet rich plasma were used as sources of platelets. Nowadays the methods of platelet concentrates (pc) production adopted in Blood Banks include the traditional method of platelet preparation by differential centrifugation of units of whole blood, besides the much more sophisticated technique of extracorporeal collection of pc with improved immunological compatibility.Manually pc are produced by the platelet rich plasmamethod, the buffy coat method and multiple bag plateletapheresis. The machine collection of pc is done by plateletapheresis or platelet elutriation
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Chen, Bijuan, Xuexian Chen, Lin Wang, Xiangbao Meng, Nihong Liu, and Zheng Xiong. "A Novel Seed Sorting Device Utilizing Vertical Centrifugation and Spectroscopy Techniques." In 2024 3rd International Conference on Innovations and Development of Information Technologies and Robotics (IDITR). IEEE, 2024. http://dx.doi.org/10.1109/iditr62018.2024.10554317.

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Atagi, Yuya, Yuya Akimoto, Fumiya Nagae, et al. "Development of Wireless Tomography System to Measure Solid-Liquid Flow in Rotating Body." In 2017 25th International Conference on Nuclear Engineering. American Society of Mechanical Engineers, 2017. http://dx.doi.org/10.1115/icone25-66934.

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A wireless tomography system has been developed to measure the real-time behavior of solid-liquid two phase flow in the centrifuge for controlling a rotational velocity and particle supply rate. The purpose of this study is development of real-time behavior of solid-liquid two phase flow measurement technique and image conductivity distribution in rotating body because a technique for measuring the behavior of solid-liquid two phase flow in the centrifuges has not existed yet. In this study, a device to measure wirelessly behavior of solid-liquid two phase flow in stationary body has developed
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van Marwijk-Kooy, M., A. C. Dullemond-Westland, H. C. van Prooijen, M. I. Riemens, and J. W. N. Akkerman. "INCREASED SENSITIVITY OF A PLATELET-SUBPOPULATION TO CRYOPRESERVATION." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644595.

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When cryopreserved human platelets are stimulated with a high dose of collagen (40 μg/ml), about 50% of the cells fail to aggregate, as measured by counting the disappearance of single platelets. This suggests the presence of a subpopulation with increased susceptibility to cryopreservation-induced cell damage. In an attempt to identify this subfraction fresh platelet suspensions were separated into four size-dependent fractions by counterflow centrifugation. Large platelets (MPV 11.1 μm3 ) better preserved their responsiveness than small platelets (4.2 μm3) with intermediate responses for fra
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Aydar, S., S. Alataş, L. Numanoğlu, and A. Sönmezdağ. "EFFECT OF ORAL ANTICOAGULANTS ON STABLE ROSETTE FORMATION." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643271.

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Human peripheral blood T lymphacytes when cultered in the presence of mitogen Phytohemogglutinin (PHA) acquire the capacity to form E rosettes with sheep erythrocytes that are resistant to incubation at 37° C. Whereas human thymus lymphocytes form 37° C stable E rosettes. On the other hand, it is shown that the use of anticoagulants can prevent cancer metastases which brings forth the importance of explaining the relationship between the lymphocyte functions and anticoagulant action mecha-nismus. In order to investigate this relationship, we did a group af experiments with lymphocytes of norma
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Carroll, R. C. "OPPOSITIONAL REGULATION OF PLATELET CALCIUM FLUX BY cAMP-MEDIATED PHOSPHORYLATION OF GLYCOPROTEIN lb." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643630.

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Glycoprotein (GP) lb, a surface receptor for von Willebrand's factor and thrombin, is composed of two disulfide-linked subunits (alpha - 130 kDa; and beta - 25 kDa). No significance has yet been attributed to the finding that the GPIb beta subunit is the only platelet glycopeptide which is phosphorylated in situ. GPIb can be quantitatively isolated from Triton X-100 wfibTeTlatelet lysates by monoclonal antibody (AN51) to GPIb and Protein A-Sepharose. Using this technique, I find that phosphorylation of the GPIb-beta subunit increased up to three-fold upon exposure of 32P-labeled platelets to a
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Filippi, J. F., D. Arnoux, N. Tubiana, et al. "PLASMINOGEN ACTIVATOR ACTIVITY OF NORMAL AND MALIGNANT MONONUCLEAR HUMAN CELLS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643167.

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Plasminogen activators (PA) are thought to play a role in the invasive and metastatic properties of many types of cancer cells. Though, discrepancies in correlations between fibrinolytic activity and metastatic potential of malignant cells have been described.In this study, we evaluated both tissue type (tPA) and urokinase type (UK) cellular PA activities in different mononuclear cell types : normal T and B human peripheral lymphocytes, B cells from patients with chronic lymphocytic leukemia (CLL), human blood monocytes, alveolar macrophages, U 937, RAJI and JM cell 1ines.Mononuclear cells wer
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