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Journal articles on the topic 'Centrifugation'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Daramola, James Ola. "Effect of Centrifugation on Motility, Sperm Capacitation and Acrosome Reaction in Soy Bean and Avocado Seed Milk Extenders of Cryopreserved Goat Spermatozoa." Agricultura Tropica et Subtropica 50, no. 1 (2017): 13–18. http://dx.doi.org/10.1515/ats-2017-0002.

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Abstract Removal of seminal plasma by centrifugation (0 centrifugation, 1 centrifugation, 2 centrifugations, 3 centrifugations) and preservation in two different tris-extenders viz., avocado seed milk (ASM) and soy bean milk (SBM) based extenders were studied for their ability to support motility, in vitro capacitation and acrosome reaction of spermatozoa obtained from West African Dwarf (WAD) goat bucks during cryopreservation. Semen samples collected with the aid of artificial vagina were centrifuged for one, two and three times. The centrifuged samples were diluted with the two tris-extende

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2

Gallagher, Sean R. "Centrifugation." Current Protocols Essential Laboratory Techniques 00, no. 1 (2008): 5.1.1–5.1.16. http://dx.doi.org/10.1002/9780470089941.et0501s00.

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3

Jo, Chris H., Young Hak Roh, Ji Eun Kim, Sue Shin, and Kang Sup Yoon. "Optimizing Platelet-Rich Plasma Gel Formation by Varying Time and Gravitational Forces During Centrifugation." Journal of Oral Implantology 39, no. 5 (2013): 525–32. http://dx.doi.org/10.1563/aaid-joi-d-10-00155.

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Despite the increasing clinical use of topical platelet-rich plasma (PRP) to enhance tissue healing and regeneration, there is no properly standardized method of autologous PRP gel preparation. This study examined the effect of the centrifugation time and gravitational force (g) on the platelet recovery ratio of PRP and determined the most effective centrifugation conditions for preparing PRP. Two-step centrifugations for preparing PRP were used in 39 subjects who had consented prior to the study's start. The separating centrifugation (Step 1, used to separate whole blood into its two main com

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4

Yuliandari, Aisyara, Prima Octafia Damhuri, Tiur Sherly Margaretta, Sarah Ester Priskilla, and Hartini H. "EVALUATION OF PLATELET RICH PLASMA (PRP) PREPARATION PROCEDURE." JURNAL ANALIS LABORATORIUM MEDIK 8, no. 2 (2023): 94–100. http://dx.doi.org/10.51544/jalm.v8i2.4509.

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The success of PRP therapy in repairing tissue damage is influenced by the PRP preparation procedure. Currently, there’s no standardization of PRP preparation procedures, and various techniques are used, such as the use of anticoagulants and different centrifugation speeds. This study aimed to evaluating the PRP preparation procedures based on the centrifugations steps (single and double centrifugation) and the use of anticoagulants variation (sodium citrate, EDTA and ACD-A). This study was an experimental study and used blood samples from respondents. The selected respondents must meet inclus

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5

Janky, Kristen L., and Neil T. Shepard. "Unilateral Centrifugation." Otology & Neurotology 32, no. 1 (2011): 116–21. http://dx.doi.org/10.1097/mao.0b013e3181ff7549.

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6

Knight, Pamela. "Continuous Centrifugation." Nature Biotechnology 6, no. 11 (1988): 1344–45. http://dx.doi.org/10.1038/nbt1188-1344.

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7

Kim, Mu-Young, and Hyun-Jung Han. "Optimization of a Two-Step Centrifugation Protocol for Bovine Platelet-Rich Plasma." Acta Veterinaria 72, no. 3 (2022): 375–87. http://dx.doi.org/10.2478/acve-2022-0030.

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Abstract Platelet-rich plasma ( PRP), an autologous platelet concentrated in plasma, is a source of diverse growth factors and is extensively utilized to promote tissue healing. Most of the clinical and laboratory investigations in veterinary medicine have focused on horses and dogs. Consequently, the types and detailed conditions of the PRP preparation method are based on those species. However, in bovine medicine, only a few studies have investigated the species-specific characteristics of bovine platelets. The aim of this study was to optimize the conditions of a two-step centrifugation met

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8

Xu, Shujing, and Ali Nadim. "Oscillatory counter-centrifugation." Physics of Fluids 28, no. 2 (2016): 021302. http://dx.doi.org/10.1063/1.4939988.

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9

PITTS, JIM E. "Crystallization by centrifugation." Nature 355, no. 6356 (1992): 117. http://dx.doi.org/10.1038/355117a0.

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10

ABAD-ZAPATERO, CELE. "Crystallization by centrifugation." Nature 356, no. 6368 (1992): 392. http://dx.doi.org/10.1038/356392b0.

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11

PITTS, JIM E. "Crystallization by centrifugation." Nature 356, no. 6368 (1992): 392. http://dx.doi.org/10.1038/356392c0.

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12

Wood, EJ. "Basic centrifugation techniques." Biochemical Education 19, no. 3 (1991): 156–57. http://dx.doi.org/10.1016/0307-4412(91)90065-g.

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13

Miró, Jordi, and Marion Papas. "Improvement of cryopreservation protocol in both purebred horses including Spanish horses." Spanish Journal of Agricultural Research 16, no. 4 (2019): e0406. http://dx.doi.org/10.5424/sjar/2018164-13677.

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There is a widely held belief that the semen of Purebred Spanish Horses (PRE) is of generally poorer quality than that of other breeds, and survives cryopreservation less well. To determine whether this is the case, sperm concentration, viability and morphological abnormalities were examined in a total 610 fresh ejaculates from 64 healthy PRE (N=47) and non-PRE stallions (N=17). Sperm concentration and viability were then re-examined after pre-freezing centrifugation, and once again after freezing-thawing. No differences were observed between the PRE and non-PRE stallions in terms of any sperm

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14

Wiig, Helge, Knut Aukland, and Olav Tenstad. "Isolation of interstitial fluid from rat mammary tumors by a centrifugation method." American Journal of Physiology-Heart and Circulatory Physiology 284, no. 1 (2003): H416—H424. http://dx.doi.org/10.1152/ajpheart.00327.2002.

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Access to interstitial fluid is of fundamental importance to understand tumor transcapillary fluid balance, including the distribution of probes and therapeutic agents. Tumors were induced by gavage of 9,10-dimethyl-1,2-benzanthracene to rats, and fluid was isolated after anesthesia by exposing tissue to consecutive centrifugations from 27 to 6,800 g. The observed51Cr-EDTA (extracellular tracer) tissue fluid-to-plasma ratio obtained from whole tumor or from superficial tumor tissue by centrifugation at 27–424 g was not significantly different from 1.0 (0.92–0.99), suggesting an extracellular o

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15

Peterson, Brandon W., Prashant K. Sharma, Henny C. van der Mei, and Henk J. Busscher. "Bacterial Cell Surface Damage Due to Centrifugal Compaction." Applied and Environmental Microbiology 78, no. 1 (2011): 120–25. http://dx.doi.org/10.1128/aem.06780-11.

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ABSTRACTCentrifugal damage has been known to alter bacterial cell surface properties and interior structures, including DNA. Very few studies exist on bacterial damage caused by centrifugation because of the difficulty in relating centrifugation speed and container geometry to the damage caused. Here, we provide a simple, versatile method of analysis for describing the compaction of bacteria during centrifugation based on a proposed centrifugation coefficient,C. Values ofCcan be related to different bacterial cell surface properties. Changing the geometry of the centrifugation container or cen

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16

Jobs, Magnus, W. Mathias Howell, and Anthony J. Brookes. "Creating Arrays by Centrifugation." BioTechniques 32, no. 6 (2002): 1322–29. http://dx.doi.org/10.2144/02326mt03.

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17

Peters, Richard, and Robert S. Sikorski. "Centrifugation on the Web." Nature Biotechnology 14, no. 7 (1996): 899. http://dx.doi.org/10.1038/nbt0796-899a.

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18

SUZUKI, Yoshihisa, Tsutomu SAWADA, Atsushi MORI, and Katsuhiro TAMURA. "Colloidal Crystallization by Centrifugation." KOBUNSHI RONBUNSHU 64, no. 3 (2007): 161–65. http://dx.doi.org/10.1295/koron.64.161.

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19

Lakhtina, E. V. "Centrifugation of dilute ferrofluids." Physics Procedia 9 (2010): 221–23. http://dx.doi.org/10.1016/j.phpro.2010.11.049.

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20

Wood, EJ. "An Introduction to Centrifugation." Biochemical Education 19, no. 3 (1991): 157. http://dx.doi.org/10.1016/0307-4412(91)90066-h.

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21

Quinn, Paul. "An introduction to centrifugation." Trends in Cell Biology 1, no. 2-3 (1991): 76. http://dx.doi.org/10.1016/0962-8924(91)90097-s.

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22

S.E.H. "An introduction to centrifugation." Trends in Biochemical Sciences 16 (January 1991): 392. http://dx.doi.org/10.1016/0968-0004(91)90160-w.

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23

Ning, Li, Roberto Camassa, Robert E. Ecke, and Francesco Venneri. "Solutal Separation under Centrifugation." Separation Science and Technology 33, no. 4 (1998): 551–67. http://dx.doi.org/10.1080/01496399808544996.

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24

Schneider, Cornelia M., and Helmut Cölfen. "Analytical band centrifugation revisited." European Biophysics Journal 47, no. 7 (2018): 799–807. http://dx.doi.org/10.1007/s00249-018-1315-1.

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25

Chan, Steven H., San Kiang, and Matthew A. Brown. "One-dimensional centrifugation model." AIChE Journal 49, no. 4 (2003): 925–38. http://dx.doi.org/10.1002/aic.690490411.

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26

Prell, M., R. E. Ellerbrock, J. Stewart, M. S. Bojko, and I. F. Canisso. "Comparison of centrifugation and non-centrifugation methods to cryopreserve stallion epidydimal semen." Journal of Equine Veterinary Science 43 (August 2016): S67. http://dx.doi.org/10.1016/j.jevs.2016.06.034.

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27

Molina, Miras Alejandro, Rosales Lorenzo López, García Maria del Carmen Cerón, et al. "A new approach to finding optimal centrifugation conditions for shear-sensitive microalgae." Algal Research 44 (December 1, 2019): 101677. https://doi.org/10.1016/j.algal.2019.101677.

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A study has been conducted to assess clarification efficiency and cell damage during centrifugation, and to optimize this operation for the dinoflagellate microalga <em>Amphidinium carterae</em>. Although cells were easily recovered from the cell suspension, cell damage was observed in some experiments once the cells had sedimented. Cell damage depends on both the residence time of the cells in the pellet and on the g-force applied. 2D Computer fluid dynamics simulations were carried out to simulate and predict microalgal cell settling times, and a dimensionless number was used to obtain an op

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28

Novia, Diana, and Binarwan Halim. "#302 : Shorter Centrifugation Time Leads to Better Sperm Quality in Swim-Up Processing Technique." Fertility & Reproduction 05, no. 04 (2023): 700. http://dx.doi.org/10.1142/s2661318223744120.

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Background and Aims: Centrifugation is one of the many factors that cause sperm DNA damage in IVF semen preparation. This can occur due to the formation of reactive oxygen species (ROS) that are uncontrollable, so that the quality of sperm DNA can be damaged. This study aims to compare sperm quality results with swim-up technique under centrifugation times of 5 and 10 minutes. Method: This study involved 50 patients at the Halim Fertility Center clinic from June 2020 to October 2020. The samples obtained were processed using the swim-up method. Samples were divided into 3 groups, namely contro

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29

Fallatah, Majed Hashem, Aaied Falah Al Otaibi, Randa Rajallah Aljohani, Waad Jamal Ghabban, and Alaa Ali Alshehri. "Evaluation of Centrifugation Technique and the Effect of Centrifugation Condition on the Laboratory Samples." JOURNAL OF HEALTHCARE SCIENCES 04, no. 12 (2024): 1003–9. https://doi.org/10.52533/johs.2024.41248.

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Centrifugation is a critical laboratory technique used to separate components within a mixture based on density differences. The efficiency and reliability of this process depend on the precise control of variables such as speed, time, temperature, and rotor design. Improper centrifugation settings can compromise sample integrity, leading to analytical inaccuracies. High-speed centrifugation, while effective for rapid separation, often imposes mechanical stress on samples, resulting in hemolysis or cellular damage. Time duration is equally significant, as insufficient centrifugation can cause

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30

Aukland, Knut, Olav Tenstad, and Helge Wiig. "Distribution spaces for hyaluronan and albumin in rat tail tendons." American Journal of Physiology-Heart and Circulatory Physiology 281, no. 4 (2001): H1589—H1597. http://dx.doi.org/10.1152/ajpheart.2001.281.4.h1589.

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A low concentration of hyaluronan (HA) in lymph compared with tissue suggests a large bound fraction. To investigate the distribution and mobility of HA and serum albumin (Alb), we eluted the rat tail tendon with a series of l5 successive centrifugations, each preceded by the addition of 0.15 M NaCl (15% of initial wet wt). The eluate concentration fell exponentially versus the accumulated eluate, allowing estimation of the maximal elutable amount (EHA and EAlb). Alb elution was practically complete from a space of ≈28% of wet wt at all centrifugation rates. Twenty percent of HA was elutable a

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31

Kim, Y. G., S. Bech-Nielsen, J. C. Gordon, R. D. Slemons, and E. Spangler. "Comparison of two methods for isolation of Mycobacterium paratuberculosis from bovine fecal samples." American Journal of Veterinary Research 50, no. 7 (1989): 1110–13. https://doi.org/10.2460/ajvr.1989.50.07.1110.

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SUMMARY Fecal samples from 131 cattle clinically suspect for paratuberculosis were cultured bacteriologically, using the traditional sedimentation processing method and a processing method that included a centrifugation step. Of 16 samples that were contaminated, 6 were culture-positive on at least 1 medium and by 1 processing method. Ten of 131 (7.6%) fecal samples processed by both methods were lost because of contamination. The number of culture-positive samples (using both processing methods) were 65 of 121 (53.7%) on media without miconazole and 60 of 121 (49.6%) on media with miconazole.

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32

Luschei, Edward C., Douglas D. Buhler, and Jack H. Dekker. "Effect of separating giant foxtail (Setaria faberi) seeds from soil using potassium carbonate and centrifugation on viability and germination." Weed Science 46, no. 5 (1998): 545–48. http://dx.doi.org/10.1017/s0043174500091074.

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Changes in weed seedbank composition are often monitored by removing seeds from soil samples. One extraction method accomplishes this by creating a slurry of soil and a concentrated inorganic salt solution. Centrifugation is then used to separate constituents of differing densities. We have found that centrifugation of giant foxtail seeds in 3.2 M potassium carbonate solution as conducted in a centrifugation/flotation extraction method can reduce viability as measured by germination and tetrazolium tests. In one experiment, centrifugation/flotation separation reduced germination of giant foxta

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33

Miró, Jordi, and Ester Taberner. "Effect of short-term conservation temperature, with or without centrifugation, on the survival and motility of Catalonian donkey spermatozoa." Spanish Journal of Agricultural Research 18, no. 1 (2020): e0402. http://dx.doi.org/10.5424/sjar/2020181-15740.

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Aim of study: To analyze the effect of three short-term storage temperatures with or without removing seminal plasma on the survival and motility of donkey sperm and the response to refrigeration and centrifugation of the different spermatozoa subpopulations.Area of study: North-eastern Spain (Catalonia).Material and methods: Semen from seven Catalonian jackasses was diluted with a skimmed milk-based (Kenney) extender and different treatments were obtained: FRESH semen, FRESH semen immediately centrifuged to remove the seminal plasma before resuspension in Kenney extender (FRESH+CENTRIFUGATION

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34

Majid, Nurcholis. "PENGARUH VARIASI WAKTU SENTRIFUGASI NILAI HEMATOKRIT MENGGUNAKAN METODE MIKROHEMATOKRIT DI UNIVERSITAS BINAWAN." Jurnal SainHealth 7, no. 2 (2023): 51. http://dx.doi.org/10.51804/jsh.v7i2.14334.51-57.

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Hematocrit is the total volume of red blood cells in 100 ml of blood expressed as a percentage of blood volume. Hematocrit examination is needed to help diagnose diseases such as anemia and dengue hemorrhagic fever (DHF) to determine the total volume of red blood cells or erythrocytes. The normal value for hematocrit examination is 40-54% for males and 37-47% for females. The purpose of this study was to determine the effect of variations in centrifugation time on hematocrit values using the microhematocrit method. This type of research is quantitative using experimental method with cross sect

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35

Maharani, Eva Ayu, and Dewi Astuti. "Comparison of Single Centrifugation, Double Centrifugation and Turn down-Turn up Techniques for Platelet-Rich Plasma Quality." Althea Medical Journal 9, no. 3 (2022): 180–84. http://dx.doi.org/10.15850/amj.v9n3.2628.

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Background: Platelet-rich plasma (PRP) is a new concept used in medical world, especially for wound healing. The main process that affects the PRP quality is the centrifugation process. This study aimed to assess the PRP separation process and determine the best technique of various centrifugation process.Methods: This experimental study used acid citrate dextrose (ACD) blood taken from 11 healthy respondents and compared three-techniques including the single centrifugation (SC), the double centrifugation (DC), and the double centrifugation turn down - turn up (DC-TDTU) techniques. The quality

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36

Lebl, Michal. "Centrifugation Based Automated Synthesis Technologies." JALA: Journal of the Association for Laboratory Automation 8, no. 3 (2003): 30–35. http://dx.doi.org/10.1016/s1535-5535-04-00267-9.

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Centrifugation is a powerful method for solid-liquid separation. It can be applied in numerous ways to simplify solid phase synthetic procedures. At the same time, centrifugation is the only totally parallel technique which can be scaled up for processing volume or number of simultaneously run reactions, without the limitation of overpressure or vacuum-driven filtration-based systems. We have developed synthesizers based on the power of centrifugation — peptide and small organic molecule synthesizers utilizing cotton as the synthetic substrate and inclusion volume chemistry, synthesizers for a

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37

Xu, Xufeng, and Helmut Cölfen. "Ultracentrifugation Techniques for the Ordering of Nanoparticles." Nanomaterials 11, no. 2 (2021): 333. http://dx.doi.org/10.3390/nano11020333.

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A centrifugal field can provide an external force for the ordering of nanoparticles. Especially with the knowledge from in-situ characterization by analytical (ultra)centrifugation, nanoparticle ordering can be rationally realized in preparative (ultra)centrifugation. This review summarizes the work back to the 1990s, where intuitive use of centrifugation was achieved for the fabrication of colloidal crystals to the very recent work where analytical (ultra)centrifugation is employed to tailor-make concentration gradients for advanced materials. This review is divided into three main parts. In

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38

Noguchi, Michiko, Koji Yoshioka, Hirokazu Hikono, Gentaro Iwagami, Chie Suzuki, and Kazuhiro Kikuchi. "Centrifugation on Percoll density gradient enhances motility, membrane integrity and in vitro fertilizing ability of frozen–thawed boar sperm." Zygote 23, no. 1 (2013): 68–75. http://dx.doi.org/10.1017/s0967199413000208.

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SummaryThe effects of Percoll density gradient centrifugation on sperm quality, in vitro fertilizability and developmental capacity of frozen–thawed boar sperm were evaluated. Two-step density gradient centrifugation by Percoll enhanced significantly the motility parameters of sperm compared with a simple centrifugation procedure. Percentages of motile sperm and sperm with intact plasma and acrosome membranes after Percoll separation were significantly greater than those after simple centrifugation. The rates of penetration, cleavage and blastocyst formation after in vitro fertilization were s

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39

Bos, Jelte E., and Bruno J. Correia Grácio. "Perceived radial translation during centrifugation." Journal of Vestibular Research 25, no. 3,4 (2015): 119–24. http://dx.doi.org/10.3233/ves-150555.

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40

Hodgson, John. "Centrifugation Takes a New Turn." Nature Biotechnology 9, no. 7 (1991): 628–29. http://dx.doi.org/10.1038/nbt0791-628.

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41

Agostini, Tommaso, Giuseppe Spinelli, Raffella Perello, Daniele Bani, and Giulia Boccalini. "Analysis of Lipoasiprated Following Centrifugation." Journal of Craniofacial Surgery 27, no. 6 (2016): 1489–93. http://dx.doi.org/10.1097/scs.0000000000002834.

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42

Mostowfi, Farshid, Kentaro Indo, Oliver C. Mullins, and Richard McFarlane. "Asphaltene Nanoaggregates Studied by Centrifugation†." Energy & Fuels 23, no. 3 (2009): 1194–200. http://dx.doi.org/10.1021/ef8006273.

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43

Lee, James C. "Review of Rickwood, Preparative Centrifugation." Biophysical Journal 67, no. 6 (1994): 2558. http://dx.doi.org/10.1016/s0006-3495(94)80745-3.

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44

Chewter, Les, and Michael Golombok. "Convective Flow during Gas Centrifugation." Industrial & Engineering Chemistry Research 43, no. 20 (2004): 6626–28. http://dx.doi.org/10.1021/ie049628z.

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45

Lebl, M. "Centrifugation Based Automated Synthesis Technologies." Journal of the Association for Laboratory Automation 8, no. 3 (2003): 30–35. http://dx.doi.org/10.1016/s1535-5535(04)00267-9.

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46

Anand, CV. "Preparative centrifugation: A practical approach." Biochemical Education 22, no. 3 (1994): 165. http://dx.doi.org/10.1016/0307-4412(94)90061-2.

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47

Roberts, W. L. "Gas centrifugation of research isotopes." Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment 282, no. 1 (1989): 271–76. http://dx.doi.org/10.1016/0168-9002(89)90151-4.

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48

Quinn, Paul. "Preparative centrifugation: A practical approach." Trends in Cell Biology 3, no. 8 (1993): 285. http://dx.doi.org/10.1016/0962-8924(93)90063-7.

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49

Celorie, Jay A., Ted S. Vinson, Sandra L. Woods, and Jonathan D. Istok. "Modeling Solute Transport by Centrifugation." Journal of Environmental Engineering 115, no. 3 (1989): 513–26. http://dx.doi.org/10.1061/(asce)0733-9372(1989)115:3(513).

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50

Morrell, JM, and M. Wallgren. "Colloid Centrifugation of Boar Semen." Reproduction in Domestic Animals 46 (August 26, 2011): 18–22. http://dx.doi.org/10.1111/j.1439-0531.2011.01866.x.

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