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Mimun, G. M. F. "CDC5-MEDIATED PHOSPHORYLATION OF CLB2, CDC14 AND/OR CFI1 IS REQUIRED FOR THE NUCLEOLAR RELEASE OF CDC14." Doctoral thesis, Università degli Studi di Milano, 2011. http://hdl.handle.net/2434/156038.
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In budding yeast, the protein phosphatase Cdc14 is a critical regulator of exit from mitosis. Cdc14 activity is regulated by changes in its subcellular localization. The phosphatase is sequestered in the nucleolus from G1 up to metaphase by binding to a competitive inhibitor called Cfi1. During anaphase, Cdc14 is released from its inhibitor by the sequential activation of two signaling networks, the FEAR network and the MEN. Several observations suggest that phosphorylation of Cdc14 and/or Cfi1 is responsible for the dissociation of Cdc14 from Cfi1. Three kinases play a relevant role in regulating Cdc14 release: the polo-like kinase Cdc5, the Clb2-Cdk complex and the MEN kinase Dbf2. The aim of my project was to assess the relevance of the phosphorylation of Cdc14 and Cfi1 for Cdc14 release and to investigate the contribution of the mentioned kinases to this process. By modulating the kinases of interest, we found that a correlation exists between the release of Cdc14 from the nucleolus and the phosphorylation of both Cdc14 and Cfi1. Our results suggest a role for Cdc5 in promoting the phosphorylation of Cdc14 and for Clb-Cdk and MEN in promoting the phosphorylation of Cfi1. Moreover we propose that Cfi1 phosphorylation by Clb-Cdk or MEN serves as a priming step to build up the Cdc5-mediated phosphorylation events. Our data also suggest that Clb2-Cdk is not sufficient to promote Cfi1 phosphorylation and that phosphorylation of Clb2 by Cdc5 could enable the protein to perform its function in the FEAR network, likely to promote Cfi1phosphorylation.
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Holmér, Fredrik. "CFIL-Sundbyberg." Thesis, KTH, Arkitektur, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-187883.
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Jag läste uppgiften som ett nutida ABF-hus dit man kan komma för att lära sig allt från odling, metallslöjd och yoga till omvårdnad, drama och konst. Jag tycker också att platsen har ett intressant strategiskt läge nära Sundbybergs station med mycket goda kommunikationer och precis i den punkt där bostäder möter, shoppinggator och industriområdet Solna business park. Här finns alltså alla möjligheter för nya spännande möten och utbyten. För att ta tillvara denna möjlighet så har jag skapat ett torgrum med kopplingar till de olika vägarna som kommer mest naturligt från de olika områdena. Detta för att jag framför allt tycker att det saknas platser av lugn och avslappning i området. Ett parkliknande torg skulle då kunna fylla denna lucka då platsen är väldigt tyst och lugn, har delvis mycket goda solförhållanden och programmet för CFIL innehåller inomhus- och utomhusodling. En win-win alltså. Programmet innehåller också ett café men man skulle lika gärna kunna ta med sig egen lunch att äta på platsen.This project is about a centre for informal learning, CFIL in Sundbybergs kommun. I read the task as a contemporary ABF-house where people can come to learn everything from cultivation, metalwork and yoga to nursing, drama and art. I also think that the place has an interesting strategic position close to Sundbyberg station with very good transport links and just at the point where the housing meets, shopping streets and the industrial area Solna Business Park. So, those are all possibilities for exciting new encounters and exchanges. To take advantage of this opportunity, I have created a torgrum with links to the different paths that come most naturally from the different areas. This is because I find that there is a lack of places of peace and relaxation in the area. A park-like square would then be able to fill this gap since the property is very quiet and calm has partly very good sun conditions and the program for CFIL includes indoor and outdoor cultivation. A win-win, that is. The program also includes a cafe but you might as well bring your own lunch to eat on the spot.
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Wang, Yonghua. "CFC1₃ transport in the atmosphere." Diss., Georgia Institute of Technology, 1995. http://hdl.handle.net/1853/25974.
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Brown, David. "Understanding the role of CFP1 at CpG islands." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:baf2e91a-4417-407a-8938-bbef1f6c411f.
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Vertebrate genomes are punctuated by CpG islands regions, which have an elevated frequency of CpG dinucleotides. CpG islands are associated with over 70% of mammalian promoters suggesting they may contribute to the regulation of transcription. However, despite being discovered over 30 years ago, the function of CpG islands is still not understood. Unlike the majority of the genome, CpG islands are resistant to DNA methylation. This provides a binding site for CFP1 which binds specifically to non-methylated DNA via its zinc-finger CXXC (zf-CXXC) domain. CFP1 is a subunit of the SET1 methyltransferase complex, and is thought to direct the activating histone modification H3K4me3 to CpG islands. Interestingly, CFP1 also contains a PHD domain which is proposed to bind the H3K4me3 mark, potentially producing a feedback loop between H3K4me3 and the SET1 complex. Although the structural basis for discrimination of non-methylated CpGs is known, it is not clear how zf-CXXC proteins distinguish CpG islands amongst the irregular nucleosomal landscape which exists within the nucleus. This thesis is focused on the role of CFP1 in the relationship between CpG islands, SET1 and H3K4me3. To address these questions, it was important to mechanistically dissect the contribution of the PHD and zf-CXXC domains. The proposal that the PHD domain of CFP1 binds selectively to H3K4me3 was confirmed by in vitro experiments, however this study demonstrates that the PHD domain is insufficient for stable interactions with chromatin. Using complementary genome-wide and live cell imaging approaches, the zf-CXXC domain shown to be required for PHD-dependent interactions. Genome-wide snapshots of binding interactions, together with spatial and temporal details, expose a surprising contribution of the SET1 complex to the nuclear mobility of CFP1, providing a new perspective on the role of CFP1 in H3K4 methylation.
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Xiao, Song. "Research on insulation performance of SF6 substitute CF31/CO2 under power frequency voltage and the influence of micro-moisture on CF31." Thesis, Toulouse 3, 2016. http://www.theses.fr/2016TOU30184/document.
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Les appareillages utilisant des gaz de coupure se sont fortement développés dans les réseaux haute et ultra-haute tension de par leur grande stabilité, leur faible maintenance, faible surface au sol, et leur configuration flexible. De nos jours, le SF6 est utilisé comme gaz principal dans la majeure partie des équipements de coupure haute tension, mais il reste un gaz à effet de serre dont les émissions sont néfastes pour l'environnement. Il devient donc indispensable de trouver des solutions alternatives au SF6 dans ces dispositifs électriques, comme son remplacement par des gaz moins polluants. Dans une optique de certification, des tests sont réalisés systématiquement pour évaluer les caractéristiques d'isolation du mélange sous une tension de fonctionnement, ici du CF3I/CO2. Les caractéristiques isolantes du gaz pur CF3I et des mélanges CF3I/CO2 sont testées sous différents types de défaut et l'influence des particules métalliques est évaluée au travers des propriétés thermodynamiques et des propriétés de transport d'un plasma CF3I contaminé par ces particules. Les performances globales du CF3I/CO2 (électriques et thermiques) sont donc évaluées sous des contraintes typiques et pour des matériaux métalliques différents. Enfin, l'impact de l'humidité (aléatoirement présente dans les dispositifs) sur la tenue diélectrique de l'appareil est étudié car sa présence peut avoir des conséquences dramatiquesA large amount of gas insulated equipment are applied widely as the key part of power system. Gas insulated equipment develop quickly and used widely in high and ultra-high voltage field owing to its high stability, less maintenance work, smaller floor space, and flexible configuration. Nowadays, SF6 is adopted as the main insulation medium of gas insulated equipment, which is considered a kind of dangerous greenhouse gas to environment. Global climate warming caused by greenhouse effect brings disastrous consequences to our living conditions. Electric devices account much for the emission of SF6, which makes it urgent to find a kind of environment-friendly substitute insulating gas. Besides, the decomposed products of SF6 under discharge may be corrosive to internal material and poisonous to power workers. In the early period of global research and development of environment-friendly insulated devices, mastering the formula and key technique of substitute gas is vital to electrical development. According to the above circumstance, systematic investigation of the insulating characteristics of CF3I/CO2 mixed gases under power-frequency voltage was carried out firstly, which could provide useful information for the best mixed ratio of CF3I/CO2 and the design of internal structure in matching devices. Then insulating characteristic of CF3I/CO2 and CF3I were tested under a kind of normal insulating defect of free metal particles defect, and the influence of metal particles on the thermodynamic characteristics and transmission properties of CF3I discharge plasma were calculated theoretically. The overall performance of CF3I/CO2 under typical defects were tested for the choice of internal metal materials of electrical devices. Finally, the influence of moisture, the main hazardous material on gas insulated equipment, on the insulating performance of CF3I were conducted experimentally and theoretically, which proved the harmful effect of moisture on the new insulating medium and provided theoretical foundation for the standard constitution of moisture content
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Beurton, Flore. "Étude de l’interaction physique et fonctionnelle entre le complexe histone méthyltransférase SET-2/SET1 et le complexe histone déacétylase SIN-3S dans l’embryon de C. elegans." Thesis, Lyon, 2018. http://www.theses.fr/2018LYSEN017.
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Les complexes histones méthyltransférases SET1, hautement conservés de la levure aux mammifères, sont ciblés aux régions promotrices par la protéine CFP1/CXXC, résultant en l’implémentation de la méthylation de la lysine 4 de l’histone H3 (H3K4me), modification post-traductionnelle influençant l’expression des gènes selon le contexte chromatinien. La présence de plusieurs complexes SET1 distincts dans différents systèmes modèles eucaryotes a compliqué l’étude de leurs fonctions dans un contexte développemental. Caenorhabditis elegans contient une seule protéine homologue de SET1, SET-2, et d’uniques homologues des autres sous-unités du complexe, RBBP5, ASH2, WDR5, DPY30 et CFP1. Cependant, la composition biochimique du complexe n’a pas été décrite. En couplant des expériences de co-immunoprécipitation avec des analyses de spectrométrie de masse, j’ai identifié le complexe SET-2/SET1 dans les embryons de C. elegans. D’autre part, j’ai montré que le complexe SET-2/SET1 co-immunoprécipite aussi un autre complexe conservé modifiant la chromatine et j’ai mis en évidence les interactions mises en jeu entre ces deux complexes. Mon analyse génétique a démontré que les mutants de perte de fonction des sous-unités des deux complexes partagent des phénotypes communs, en cohérence avec des fonctions développementales communes. Le laboratoire a également entrepris des expériences de transcriptomique et d’immunoprécipitation de la chromatine montrant un nouveau rôle de CFP-1 dans le recrutement de ce complexe au niveau de sites spécifiques de la chromatineThe highly conserved SET1 family complexes are targeted by CFP1/CXXC protein to promoter regions through multivalent interactions to implement methylation of histone H3 Ly4 (H3K4me), a modification that correlates with gene expression depending on the chromatin context. The presence of distinct SET1 complexes in multiple eukaryotic model systems has hampered studies aimed at identifying the complete array of functions of SET1/MLL regulatory networks in a developmental context. Caenorhabditis elegans contains one SET1 protein, SET-2, one MLL-like protein, SET-16, and single homologs of RBBP5, ASH2, WDR5, DPY30 and CFP1. The biochemical composition of the complex however, has not been described. Through the use of co-immunoprecipitation coupled to mass spectrometry-based proteomics, I identified the SET-2/SET1 complex in C. elegans embryos. Most importantly, I showed that the SET-2/SET1 complex also co-immunoprecipitates another conserved chromatin-modifying complex and I highlighted the interactions involved between these two complexes. My genetic analysis revealed that loss of function mutants of the two complex subunits share common phenotypes, consistent with common developmental functions. The laboratory has also undertaken transcriptomic and chromatin immunoprecipitation experiments showing that CFP-1 has a role in the binding of this complex at specific chromatin regions
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Adjei, David, and Stina Nilsson. "From project to practice : Creating conditions for digital healthcare implementation using the CFIR framework." Thesis, Umeå universitet, Institutionen för informatik, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-185741.
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Despite the phenomenal innovations and huge investments in healthcare, it is evident that some digital transformation innovations in the healthcare context fail to meet their desired outcomes. Whilst some researchers argue that this is partly due to ineffective implementation, others argue otherwise. Essentially, there is an outspoken need to evaluate implementation processes, and one way to do so is through the use of an implementation framework, where one such framework showing great potential is the Consolidated Framework for Implementation Research (CFIR). By using a qualitative case study through the lenses of the CFIR, the study carries out a summative evaluation and examination of a previously conducted implementation process in a region in the north of Sweden, with the purpose of developing a greater understanding of implementation processes in order to improve patient care, as well as experiences and outcomes of digital transformation implementations. The study successfully identifies factors of both success and challenge in relation to implementation processes, where some of the success factors include the involvement of caregivers in the process and competition of private healthcare providers, whilst some of the challenges include deficient involvement of end-users as well as lack of structure. The study unearths some lessons which can be used as a guide for future implementations within the healthcare context, but also other contexts as well.
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Hardy, Jessica. "Human cleavage factor I (CFIm) and its role in alternative polyadenylation of pre-mRNA." Thesis, University of Oxford, 2017. https://ora.ox.ac.uk/objects/uuid:a3ba5d10-b3fa-4ab7-9709-a0d642e21543.
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For many human protein-coding genes, alternative cleavage and polyadenylation (APA) of pre-mRNA generates distinct 3' untranslated regions (3'UTRs) with differing regulatory potential. Widespread 3'UTR shortening via APA occurs in proliferative cell states, including cancer, where it can lead to oncogene overexpression. There has therefore been significant interest in identifying factors which influence poly(A) site choice in different physiological states. The multi-subunit human cleavage factor I complex (CFIm), a core component of the mammalian pre-mRNA cleavage machinery, has been identified as a potential master regulator of APA, as its depletion leads to widespread 3'UTR shortening. However, mechanistic understanding of how CFIm influences poly(A) site selection, and how its activity is regulated, is lacking. In this work, gene editing was used to generate cell lines with substantial, permanent depletion of the 25 kDa or 68 kDa subunits of CFIm (CFIm25 and CFIm68), which exhibited the expected 3'UTR shortening for representative transcripts. Reversal of this 3'UTR shortening by CFIm25 or CFIm68 re-expression provided the basis for a complementation assay, which allowed various aspects of CFIm25 and CFIm68 function to be investigated in vivo. The capacity of CFIm25 to recognise UGUA RNA sequences was shown to make an important contribution to poly(A) site selection transcriptome-wide, and a novel function for the C-terminal arginine/serine-rich (RS domain) of CFIm68 in poly(A) site selection was identified. The potential contribution of CFIm post-translational modification (PTM) to APA regulation was also explored. Novel acetylation sites on CFIm25 and CFIm68 were identified, as well as extensive serine phosphorylation in the CFIm68 RS domain. Complementation analysis revealed that phosphomimetic mutations in this RS domain inhibited distal poly(A) site selection, suggesting a potential role for CFIm68 phosphorylation in APA regulation. Taken together, the findings presented here provide insights into several important determinants of CFIm function, and the complementation assay developed provides a useful tool for future investigations.
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Guzmán, Bernal Sofía Belén. "Efecto de lovastatina sobre células de carcinoma mamario canino (Canis lupus familiaris) CF41.Mg." Tesis, Universidad de Chile, 2018. http://repositorio.uchile.cl/handle/2250/171004.
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Memoria para optar al Título Profesional de Médico VeterinarioEl cáncer mamario es la neoplasia que se presenta con mayor frecuencia en la hembra canina reproductivamente entera. Diversos investigadores han demostrado que lovastatina exhibe un efecto antitumoral in vitro sobre diversas líneas celulares neoplásicas humanas, sin embargo, su potencial efecto aún no ha sido evaluado sobre células de carcinoma mamario canino. Lovastatina inhibe a la enzima hidroxi-metil-glutaril coenzima A (HMG-CoA) reductasa, bloqueando la biosíntesis de colesterol y de sus isoprenoides farnesil pirofosfato (FPP) y geranil-geranil pirofosfato (GGPP), importantes mediadores de algunas vías de transducción de señales asociadas a sobrevida celular. El objetivo de este estudio fue determinar los efectos de lovastatina sobre la proliferación, invasión y apoptosis de células de carcinoma mamario canino CF41.Mg. Se cultivaron células CF41.Mg y se trataron con lovastatina (0-20 μM) por diferentes tiempos. Luego, se evaluó viabilidad celular (método de reducción de MTS), invasión celular (método Transwell) y apoptosis (ensayo de Anexina V/Yoduro de Propidio). Lovastatina indujo una disminución significativa en la viabilidad celular (p ≤ 0.05), efecto que fue dependiente de su concentración y el tiempo. La invasión celular fue inhibida en respuesta a concentraciones no citotóxicas de lovastatina (p ≤ 0.05). Además, esta droga ejerció un efecto pro-apoptótico dependiente del tiempo (p ≤ 0.05). Estos resultados demuestran que lovastatina cumpliría un importante papel sobre la proliferación y capacidad invasiva de células de carcinoma mamario canino CF41.Mg, lo cual sustenta futuros estudios clínicos con esta droga
Mammary cancer is the tumor that occurs most frequently in the reproductively intact female dog. Several researchers have shown that lovastatin exhibits an in vitro antitumor effect on various human neoplastic cell lines, however, its potential effect has not yet been evaluated in canine mammary carcinoma cells. Lovastatin inhibits HMG-CoA reductase, blocking the biosynthesis of cholesterol and its isoprenoids FPP and GGPP, important mediators of some signal transduction pathways associated with cell survival. The aims of this study were to determine the effects of lovastatin on the proliferation, invasion and apoptosis of canine mammary carcinoma cells CF41.Mg. CF41.Mg cells were cultured and treated with lovastatin (0-20 μM) for different times. Then, cell viability (MTS reduction method), cell invasion (Transwell method) and apoptosis (Annexin V/Propidium Iodide assay) were evaluated. Lovastatin induced a significant decrease in cell viability (p ≤ 0.05), an effect that was dependent on its concentration and time. Cell invasion was inhibited in response to non-cytotoxic concentrations of lovastatin (p ≤ 0.05). In addition, lovastatin exerted a pro-apoptotic effect dependent on time (p ≤ 0.05). These results demonstrate that lovastatin would play an important role in the proliferation and invasive capacity of canine mammary carcinoma cells CF41.Mg, which supports future clinical studies with this drug
Proyecto Fondecyt 11110148
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Terraciano, Paula Barros. "Vitrificação versus congelamento lento não automatizado em tecido ovariano de camundongos CF1." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2016. http://hdl.handle.net/10183/151510.
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Introdução: a alta prevalência do câncer e o aumento significativo da sobrevivência em longo prazo geraram interesse quanto à preservação da fertilidade em mulheres jovens expostas a quimioterapia e radioterapia. Neste sentido estudos de congelamento de tecido ovariano para posterior transplante, abriram uma nova perspectiva de aplicação no tratamento e prevenção da infertilidade feminina. Objetivos: comparar dois protocolos de congelamento de tecido ovariano, um lento não automatizado e um por vitrificação, com o intuito de avaliar a viabilidade dos tecidos para posterior transplante autólogo. Método: Foram utilizadas 30 camundongos fêmea CF1 com aproximadamente 8 semanas e pesando 29,29g±2,9. •Os ovários extraídos foram vitrificados ou congelados, mantidos em nitrogênio líquido por 30 dias e descongelados. Após o descongelamento, o ovário esquerdo foi destinado às análises histológicas e caracterização por imuno histoquímica para o marcador mouse vasa homologue (MVH) e o ovário direito foi utilizado para os testes de viabilidade celular com exclusão por azul de trypan. Resultados: Nas análises de Hematoxilina e Eosina (HE) foram contados folículos primordiais, primários, pré-antrais e antrais. Não houve diferença significativa na proporção de folículos primordiais, primários e pré-antrais após descongelamento entre os grupos testados. A contagem de folículos antrais foi significativamente maior no grupo de vitrificação (p = 0,004). No ensaio de imunohistoquímica para o marcador MVH, folículos MVH + e MVH- foram contados e comparados com o número total de folículos. O grupo congelamento lento apresentou maior número de células não marcadas (p = 0,012). Conclusão: Embora ambos os protocolos tenham apresentado resultados semelhantes na análise histológica das contagens foliculares, o protocolo de vitrificação foi significativamente melhor para preservar a população de células tronco ovarianas.Introduction: The high prevalence of cancer and the significant increase in long-term survival have generated interest as the preservation of fertility in young women exposed to chemotherapy and radiotherapy. Experimental techniques have been tried in an attempt to reverse the ovarian failure induced by these treatments. In this regard studies of ovarian tissue freezing for subsequent transplantation disclose a new application perspective in the treatment and prevention of female infertility. Objective: two ovarian tissue freezing protocols were tested, a non-automated slow-freezing and by vitrification, in order to assess the viability of the tissues for subsequent autologous transplantation. Methods: as ovaries donors, were used 30 female CF1 mice approximately 8 weeks and weighing 29,29g±2,9. • The ovaries were vitrified or frozen, stored in liquid nitrogen for 30 days and thawed. After thawing, the left ovary was intended for histological and immunohistochemical characterization by histochemical marker for MVH and right ovary was used for the tests with cell viability by trypan blue exclusion. Results: In HE slides was counting primordial, primary, pre antral and antral follicles. No significant difference was found in the proportion of high-quality primordial, primary and pre antral follicles after thawing/warming in the slow-freezing and vitrification group, respectively. The antral follicle counting was significant higher in vitrification group (p=0,004). In immunohistochemistry assay for MVH Antibody , MVH+ and MVH- follicles were counted and compared with the total number of follicles and slow freeze group had a higher number of not marked cells (p=0,012). Conclusion: Although both protocols showed similar results in the histological analysis for follicular counts, the vitrification protocol was significantly better for preserve the ovarian stem cell population.
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Gomide, Paula Regina Silva. "Avaliação do canal flare index (CFI) femoral de diferentes raças de cães /." Jaboticabal, 2019. http://hdl.handle.net/11449/191439.
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Orientador: Bruno Watanabe MintoResumo: As disfunções degenerativas do quadril representam uma das condições ortopédicas mais prevalentes e importantes em cães, uma vez que são potencialmente debilitantes e de manejo desafiador. A substituição da articulação por componentes protéticos, conhecida como artroplastia total do quadril (ATQ), é uma possibilidade de tratamento capaz de preservar a funcionalidade do membro, com movimentação articular livre de dor. Para que a ATQ seja bem-sucedida e livre de complicações, é de suma importância o conhecimento detalhado da morfologia dos componentes anatômicos da articulação coxofemoral. A presente tese propõe avaliar o índice de alargamento femoral (CFI – Canal Flare Index) em quatro raças específicas de cães. Os objetivos principais do estudo foram identificar a existência de valor de referência ou padronização do CFI para cada raça, além de comparar os valores obtidos por meio de mensurações com referenciais anatômicos distintos, e ainda avaliar a variabilidade intra e interobservador ao medir o CFI femoral. Foram utilizadas 35 radiografias de articulações coxofemorais de cães das raças Rottweiler, Pastor Alemão, Golden Retriever e Retriever Labrador, de ambos os gêneros, configurando 70 unidades experimentais (fêmures direito e esquerdo) submetidas ao cálculo do CFI. Três avaliadores realizaram as mensurações a partir das radiografias em projeções ventrodorsais. Foi determinado o valor objetivo (CFIob) e subjetivo (CFIsub) do CFI e verificou-se que houve diferença signifi... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Degenerative hip dysfunctions represent one of the most prevalent and important orthopedic conditions in dogs, as they are potentially debilitating and challenging. Replacement of the diseased joint by prosthetic components, known as total hip arthroplasty (THA), is a possibility of treatment that can preserve limb functionality, with joint movement free of pain. In order THA to be successful and free of complications, detailed knowledge of the morphology of the anatomical components of the hip joint is of paramount importance. This thesis proposes to evaluate the femoral canal flare index (CFI) in four specific dog breeds. The main objectives of the study were to identify the existence of a reference value or standardization of the CFI for each race, as well to compare the values obtained through measurements with distinct anatomical references, and to evaluate the intra and interobserver variability when measuring the femoral CFI. Thirty-five radiographs of hip joints of Rottweiler, German Shepherd, Golden Retriever and Retriever Labrador dogs of both genders were used, configuring 70 experimental units (right and left femurs) submitted to CFI calculation. Three evaluators performed measurements from radiographs in ventrodorsal projections. The objective (CFIob) and subjective (CFIsub) CFI values were determined and it was found that there was a significant difference of CFIob between the Golden Retriever and German Shepherd breeds and CFIsub between the Golden Retriever, G... (Complete abstract click electronic access below)
Doutor
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Nzila, Michelo M. "Cooperative financial institutions (CFIs) as a source of development finance - a case study on Sub-Saharan Africa." Master's thesis, University of Cape Town, 2014. http://hdl.handle.net/11427/29056.
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The lack of access to finance is cited as one of the major barriers preventing developing countries from attaining economic development. While traditional sources of financing such as Official Development Assistance (ODA), Foreign Direct Investment (FDI) and Remittances have done much to alleviate the problem, they have left what is termed the missing middle; a financing gap created by failure to provide financing particularly for Micro, Small and Medium Enterprises (MSMEs) and for the poor in the subject countries. The major impediments have included lack of collateral, inadequate training and business knowledge and risk aversion on the part of traditional financial institutions such as banks. Further, domestic resource mobilization endeavours have concentrated on tax reforms to improve governments' revenue collection and administration, leaving personal savings aggregation unattended. This financing gap is despite knowledge that MSMEs possess the most potential for employment creation, thus poverty alleviation for the masses. Cooperative Financial Institutions have been in existence for a long time and have the potential to provide innovative solutions in addressing the problem at hand. They have however, received little attention and recognition and the historical association with agriculture and the older generation has limited their outreach and impact. This study is thus intended to explore whether CFIs can bridge the financing gap for MSMEs in Sub-Saharan Africa.
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Cui, Xiaojiang. "Identification of cotton fiber stage-specific genes and characterization of a potential plant callose synthase subunit CFL1 /." Digital version accessible at:, 1999. http://wwwlib.umi.com/cr/utexas/main.
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Tamagnini, Filippo. "EKF based State Estimation in a CFI Copolymerization Reactor including Polymer Quality Information." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2020. http://amslaurea.unibo.it/20235/.
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State estimation is an integral part of modern control techniques, as it allows to characterize the state information of complex plants based on a limited number of measurements and the knowledge of the process model. The benefit is twofold: on one hand it has the potential to rationalize the number of measurements required to monitor the plant, thus reducing costs, on the other hand it enables to extract information about variables that have an effect on the system but would otherwise be inaccessible to direct measurement.
The scope of this thesis is to design a state estimator for a tubular copolymerization reactor, with the aim to provide the full state information of the plant and to characterize the quality of the product.
Due to the fact that, with the existing set of measurements, only a small number of state variables can be observed, a new differential pressure sensor is installed in the plant to provide the missing information, and a model for the pressure measurement is developed.
Following, the state estimation problem is approached rigorously and a comprehensive method for analyzing, tuning and implementing the state estimator is assembled from scientific literature, using a variety of tools from graph theory, linear observability theory and matrix algebra. Data reduction and visualization techniques are also employed to make sense of high dimensional information.
The proposed method is then tested in simulations to assess the effect of the tuning parameters and measured set on the estimator performance during initialization and in case of estimation with plant-model mismatch.
Finally, the state estimator is tested with plant data.
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O'Regan, Thomas Noel. "Sacred polychoral music in Rome, 1575-1621." Thesis, University of Oxford, 1988. http://ora.ox.ac.uk/objects/uuid:daa9a67e-cf31-4a1b-8d74-4b814acb6957.
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The object of this thesis is to lay open a repertory of music which has long been ignored, the music for two and more choirs composed by Roman composers of the generation of Palestrina and his immediate successors. Polychoral music is taken to mean music in which two or more independent and consistent groups of voices take part, singing separately and together; the parts should remain independent in tuttl sections, with the possible exception of the bass parts. By this definition, the first real polychoral music to be published in Rome was that by Giovanni P. da Palestrina in his Motettorum liber secundus of 1575. This is taken as the starting point for this study. Music which might have influenced Roman composers is examined, as well as eight-voice music by Roman composers which is not polychoral according to the above criteria. The development of polychoral music in the city is then traced through the reigns of the various popes from Gregory XIII to Paul V, whose death in 1621 is taken as a convenient place to end the study. Particular emphasis is laid on structural and textural aspects and the way these were adapted by successive composers. The ground for the Roman concerts to style was laid in the early experiments by composers such as Giovanni Animuccia, Palestrina and Tomas Luis de Victoria; this is traced through what is termed the 'fragmented' style of the last two decades of the sixteenth century to the full flowering of the large-scale concerts to motet after 1605. The music is studied in the context of the institutions for which it was written. The archives of these Institutions have been researched for information on performance practice, which is presented here. The broader cultural, social and religious background which spawned the idiom is also examined and polychoral music related both to the new propagandist attitude of church leaders from Gregory XIII onwards, and to a general expansion in musical activity in the city of Rome through the period under investigation. The various printed and manuscript sources for this music have been researched and the resulting catalogue of pieces by fifty or so composers who worked in the city is presented. A more detailed examination is carried out of the primary manuscript sources, from which valuable information on various aspects of the music can be obtained.
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Dorrian, Gareth. "Large scale 3-dimensional structure of the solar wind." Thesis, Aberystwyth University, 2009. http://hdl.handle.net/2160/3232306b-cf11-4c7c-81a6-4e4e635149cc.
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This thesis discusses new observations made of large scale solar wind structure during solar minimum conditions. The phenomena of interplanetary radio scintillation (IPS) is exploited and used in conjunction with white light observations from the Heliospheric Imager instruments on the STEREO spacecraft. Methodologies for combining the two techniques are developed and then used in two case studies. The first case study is an observation and analysis of complex solar wind features in the presence of a co-rotating interaction region. The second is an observation and analysis of coronal mass ejections and their associated effects. In both studies, observations were supported by in situ data from the Venus Express spacecraft. Both cases represent the first time that such phenomena have been observed at interplanetary distances using these techniques. Extremely long baseline IPS observations were also made in order to address the hypothesis that the fast solar wind flow is radial. Observations made over several years, at different times in the solar cycle and over both hemispheres demonstrate that, down to the level of sensitivity for this technique, the hypothesis still stands. Off radial flow characteristics were observed using this technique during the passage of non-ambient solar wind features across the raypath. These results demonstrate that this technique can detect off radial flow when it is present and hence reveal aspects of the behaviour of the interplanetary magnetic field under different solar wind conditions.
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Pitt, Anna Tamsin. "Auditory discrimination in dyslexia : differences between university and non-university educated individuals." Thesis, University of Oxford, 2009. http://ora.ox.ac.uk/objects/uuid:ad1dfcb0-cfe1-4d62-a603-749ccf84a0a9.
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It is still unresolved whether individuals with dyslexia suffer non-verbal auditory processing deficits that may explain their phonological problems. Many studies have shown that dyslexic individuals are poor at discriminating pure tones, and this deficit has been attributed to impaired rapid auditory processing. In order to investigate the temporal properties of auditory processing in dyslexia, I have therefore studied the pure tone discrimination abilities of dyslexics, and then analysed the effects of varying interstimulus interval, the amount of frequency difference, and the effect of adding distractor tones during the interstimulus interval. In an investigation of dyslexic individuals’ ability to remember sequences of tones or digits (tonal and digit recognition memory), Rose and Rosner (2005) found that their results were affected by the education their subjects had received. The university educated dyslexics showed little tone discrimination deficit, whereas the dyslexic participants who never attended university showed greater deficits. Therefore, another aim of this thesis was to further study these findings and to identify any auditory processing compensatory mechanisms used by dyslexics who have received higher education. In eight different auditory tasks, the majority of which I programmed, I found strong evidence of non-verbal auditory processing deficits in dyslexic individuals. The comparison of university and non-university groups showed that educational differences had clear effects on many of their abilities, and should not be underestimated. The results showed that: • In general, dyslexics had poorer auditory frequency discrimination than controls. • Dyslexics who did not attend university had lower performance on almost all the auditory tests than the university dyslexic or control groups. • Unexpectedly, university educated dyslexics were less distracted by interrupting tones during frequency discrimination than university educated controls. • In a tone sequence memory task, the university educated dyslexics compensated in their performance to a level not much below that of the university educated controls, and above the controls who did not go to university. • The frequency recognition tasks positively correlated with literacy abilities, and were independent of general intelligence. The strongest correlations were in the non-university dyslexic group. The implications of these results are that not only do dyslexic individuals suffer from a low level, non-linguistic, auditory processing deficit, but those who do not get to university are less able to compensate for these difficulties. It is impossible to say if the higher performance in university educated dyslexics was due to compensation, or if their presence at university was due to a lack of these deficits in the first place. Nevertheless, since university educated dyslexics were better at resisting distractions this may underlie their ability to compensate. These findings could facilitate the creation of new teaching methods to support the development of dyslexics’ compensatory skills and new non-linguistic diagnostic aids. These would help with identifying dyslexia in second languages and enable earlier testing and identification, before reading failure exerts its inevitable negative effects on children’s self-confidence, happiness and future academic potential.
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Reyes, Leiva Fernando José. "Rol de la autofagia inducida por simvastatina sobre la viabilidad de células de carcinoma mamario canino CF41. Mg." Tesis, Universidad de Chile, 2017. http://repositorio.uchile.cl/handle/2250/145882.
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Memoria para optar al Título Profesional de Médico Veterinario.
Tesis para optar al Grado de Magíster en Ciencias Animales y Veterinarias mención Patología Animal.Introducción. El cáncer mamario es el tipo de tumor más prevalente en la hembra canina. Información reciente demuestra que simvastatina exhibe un efecto antitumoral in vitro e induce autofagia sobre diversas líneas celulares neoplásicas. Sin embargo, no hay evidencia de que ocurra en células de carcinoma mamario canino. La autofagia es un proceso catabólico que permite a las células sobrevivir bajo condiciones de estrés metabólico, cumpliendo un rol dual en cáncer asociado a supresión y promoción tumoral dependiendo del contexto celular. En ese contexto, la autofagia jugaría un rol en la sobrevida y muerte de células tumorales en respuesta a agentes antitumorales. El objetivo de este estudio fue analizar los efectos de simvastatina sobre la autofagia y la viabilidad de células de carcinoma mamario canino CF41.Mg. Materiales y métodos. Células CF41. Mg fueron cultivadas y tratadas con simvastatina (1 y 5 M) por 24 horas y analizadas mediante citometría de flujo utilizando naranja de acridina. Se analizó la expresión de LC3 y beclina-1 en células tratadas con simvastatina y 3-metiladenina (inhibidor de autofagia) mediante inmunocitoquímica. Adicionalmente, se realizó un ensayo de viabilidad celular mediante el método de exclusión de azul tripan con células tratadas con simvastatina en presencia de Q-VD-OPh (inhibidor de apoptosis) y 3-metiladenina. Resultados Tanto la actividad autofágica como la expresión de LC3 y beclina-1 fueron inducidas por simvastatina (p<0.05). Estos efectos fueron revertidos por 3-metiladenina. Simvastatina inhibió la viabilidad celular en forma significativa, aún en presencia de 3-metiladenina (p<0.05). Q-VD-OPh bloqueó el efecto antiproliferativo desencadenado por simvastatina. Conclusiones El efecto antiproliferativo de simvastatina sobre células de carcinoma mamario canino CF41.Mg no es dependiente de la autofagia inducida por la misma droga. La citotoxicidad de simvastatina se explica casi completamente por la activación de caspasas
Introduction Mammary cancer is the most prevalent type of tumor in the female dogs. Recent data shows that simvastatin exhibit an antitumor effect in vitro and it is able to induce autophagy in some neoplastic cell lines. However, there is still no evidence in canine mammary cancer cells. Autophagy is a mayor catabolic process that allows the cells to survive under metabolic stress, performing a dual role in cancer associated to tumor suppression/progression depending on cellular context. Under this context, the autophagy may play a role in the survival and death of tumoral cell in response to anti-tumoral agents. The object of this study was to analyze the effects of simvastatin over the autophagy and viability of canine mammary carcinoma cells CF41.Mg. Materials and methods CF41.Mg cells were treated with simvastatin (1 y 5 M) for 24 hours and analyzed by flow cytometry using acridine orange dye. The expression of LC3 and beclin-1 in cells treated with simvastatin/3-methyladenine (autophagy inhibitor) by immunocytochemistry was analyzed. Cell proliferation assays with trypan blue dye in presence of simvastatin, Q-VD-OPh hydrate (pancaspase inhibitor) and 3-methyladenine were performed. Results Both autophagy activity and LC3/beclin-1 expression were induced by simvastatin (p<0.05). These effects were reversed by 3-methyladenine. This statin inhibited the cell viability, even in the presence of 3-methyladenine (p<0.05). Q-VD-OPh hydrate blocked the anti-proliferative effect of simvastatin. Conclusions The anti-proliferative effect of simvastatin over canine mammary carcinoma cells CF41.Mg it’s non-depending of the autophagy induced by the drug. The cytotoxicity of simvastatin it’s explained almost completely by the caspases activation
Financiamiento: Proyecto Fondecyt No. 11110148.
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Serrano, González Consuelo Francisca. "Efecto de melatonina sobre la capacidad invasiva de células de carcinoma mamario canino (Canis lupus familiaris) CF41.Mg." Tesis, UNIVERSIDAD DE CHILE, 2017. http://repositorio.uchile.cl/handle/2250/151154.
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Memoria para optar al Título Profesional de Médico VeterinarioLa neoplasia mamaria es una enfermedad que afecta comúnmente a hembras caninas. En el microambiente tumoral existe una subpoblación de células tumorales que exhiben características de troncalidad (CSC), que pueden formar esferas in vitro, resistir tratamientos antitumorales, explicando en parte la recurrencia de algunas neoplasias. Previamente, se ha descrito que esferas derivadas de células de carcinoma mamario canino CF41.Mg exhiben características de troncalidad. Melatonina ha mostrado efectos antitumorales sobre células tumorales mamarias; sin embargo, sus efectos han sido pobremente evaluados en CSC mamarias caninas. Melatonina modula la expresión de proteínas relacionadas con transición epitelio-mesénquima en CSC mamarias, como E-cadherina y OCT-4. El objetivo de este estudio fue determinar los efectos de melatonina sobre la proliferación, migración e invasión de células CF41.Mg y esferas derivadas de ellas. Se cultivaron células CF41.Mg en DMEM alto en glucosa suplementado con suero fetal bovino y L-glutamina. Las esferas se cultivaron en placas de ultra-baja adherencia con DMEM/F12 en presencia de EGF, bFGF, insulina, B27 y heparina. La proliferación (reducción de MTS) y los ensayos de migración/invasión (transwell) celular se realizaron en presencia de melatonina (0,01, 0,1 o 1 mM). Melatonina indujo un efecto antiproliferativo a 1 mM (P<0.05), sin embargo, el efecto sobre las esferas fue mayor (P<0.0001). La migración/invasión celular fue inhibida en respuesta a concentraciones no citotóxicas de melatonina (P<0.05) en ambos tipos celulares. Estos resultados indican que melatonina juega un papel en la actividad proliferativa e invasiva de células CF41.Mg, siendo un potencial agente contra CSC mamarias.
Mammary cancer is a common disease affecting female dogs. In the tumor microenvironment there is a subpopulation of cancer cells with stem cell-like features (CSC), that can form in vitro spheres and resist conventional antitumor treatments explaining in part the recurrence of some cancers. It has been previously reported that spheres derived from CF41.Mg canine mammary carcinoma cells exhibit some stemness features. Melatonin has shown antitumor effects on cancer mammary cells; nevertheless, its effects has been poorly evaluated on canine mammary CSC. Recent reports have showed that melatonin modulates the expression of proteins related to epithelial-mesenchymal transition in breast CSC, such as E-cadherin, vimentin and OCT-4. The aim of this study was to determine the effects of melatonin on proliferation, migration and invasion of canine mammary carcinoma CF41.Mg cells and spheres derived from them. CF41.Mg cells were grown in DMEM high glucose supplemented with FBS and L-glutamine. CF41.Mg-spheres were cultured in ultra-low attachment plates with serum-free DMEM/F12 in presence of EGF, bFGF, insulin, B27 and heparin. Cell proliferation (MTS reduction) and migration/invasion (transwell) assays were conducted in presence of melatonin (0.01, 0.1 or 1 mM). Melatonin induced an antiproliferative effect at 1 mM (P<0.05), however the effect on spheres was higher (P<0.0001) than in parental cells. Cell migration/invasion was inhibited in response to non-cytotoxic concentrations of melatonin (P<0.05) both in spheres and in parental cells. These results indicate that melatonin plays a role in the proliferative and invasive activity of CF41.Mg cells, representing a valuable potential agent against mammary CSC.
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Thomson, John Paterson. "Defining the protein complement of CpG islands." Thesis, University of Edinburgh, 2011. http://hdl.handle.net/1842/4885.
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In higher eukaryotes, the DNA base Cytosine can exist in a variety of modified forms when in the dinucleotide CpG. Although a methylated form tends to dominate within the genome, approximately 1% of all CpG dinucleotides are found unmodified at high densities spanning around 1Kb and tend to co-localise to the 5’ ends of around 60% of annotated gene promoters. These unique DNA sequences are known as CpG islands (CGIs) and their role within the genome to date is largely unknown. Methylation of CGIs in cancers however has been linked to silencing of associated genes implying a role in gene regulation. Furthermore these sites are also interesting as they remain specifically nonmodified within a genome rich in methylated CpG. We set out to better understand the roles for CGIs through the characterisation of any specific CGI binding proteins. Digestion of nuclei with methyl sensitive restriction enzymes facilitates the purification of CGI fragments. Subsequent immunohistochemistry on the CGI chromatin fragments along with ChIP-PCR over several CGIs revealed an enrichment of the “active” histone modifications including H3K4me3, a depletion of the “silencing” marks such as H3K27me3, as well as a group of CGI specific binding factors. These latter proteins contained a domain previously shown to bind to non-methylated CpG dinucleotides (the CXXC domain) and as such were ideal candidates for CGI specific factors, in particular a protein called Cfp1. Genome wide sequencing revealed a striking correlation between Cfp1 and H3K4me3 which were both seen at around 80% of islands. Furthermore, the presence of Cfp1/H3K4me3 at islands tended to have a negative correlation with the presence of chromatin rich in the silencing histone modification H3K27me3. Closer investigation of the Cfp1 protein reveals it to be a true non-methyl CGI binding factor in vivo and shRNA reduction of Cfp1 levels to around 10% of wild type resulted in a precipitous drop in H3K4me3 levels over CGIs without a dramatic reduction in global H3K4me3 levels. As Cfp1 has been shown to be part of the Set1 histone H3K4 methyltransferase complex responsible for this modification, this CXXC protein may be attracting this histone modifying complex and as such represents a method whereby the underlying DNA sequence (CpG) can drive the overlying epigenetic state. This study may go some way to understanding the functional significance of CGIs within the genome.
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Testa, Valerie. "Implementation of a First Responder Operational Stress Injury Clinic Using the TDF-II and CFIR Frameworks: A Paramedic Perspective." Thesis, Université d'Ottawa / University of Ottawa, 2021. http://hdl.handle.net/10393/41918.
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Background: First responders (firefighters, paramedics, and police officers) are often exposed to potentially psychologically traumatic events. When combined with insufficient social support and reduced help-seeking behaviours, such exposures may increase the risk of mental health challenges, particularly among paramedics who report the highest rates of mental disorders. Objective: The current study used the Theoretical Domains Framework (TDF) and the Consolidated Framework for Implementation Research (CFIR) to identify critical barriers and facilitators to help-seeking and accessing mental health care, and the feasibility and sustainability of a first responder clinic. Methods: Semi-structured qualitative interviews included 11 paramedics (frontline, mid-and-senior management, and union), recruited using purposive and snowball sampling. Interviews were analyzed using content and thematic analyses. The TDF and CFIR guided study design, interview content, data collection, and analysis. Results: Barriers included the complexities of stigma, confidentiality, cultural competency, and trust. Conclusions: The findings will be instrumental in developing evidence-based approaches to mental health care for paramedics.
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Birdsong, Linda L. "Development of an Instrument to Ascertain Teachers' Use of CFIP as a Tool for Improving Classroom Instruction and Learning." Thesis, Notre Dame of Maryland University, 2015. http://pqdtopen.proquest.com/#viewpdf?dispub=3684654.
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Abstract This research was designed to develop an instrument to evaluate how use of the Classroom Focused Improvement Process (CFIP) affected teachers' perceptions of using student data. The CFIP process requires that teacher teams regularly analyze student data, to strategically plan, reevaluate, and re-plan. Teams own achievement for all students. Ainsworth (2006) asserted teams must use collaboratively created short-cycle assessments to ensure common student growth. Data guide team-adjusted teaching, and professional development. Teacher belief in interim assessments' potential derives from formative assessment research, particularly studies demonstrating formative assessments and daily classroom feedback can improve teaching and student performance (Goertz, Olah, & Riggan, 2009). Engaging in this process, perhaps teachers can shift data perceptions from an accountability tool, to a means to improve student learning. The No Child Left Behind Act stipulated every child should test on-grade-level in reading and mathematics by 2014, evidenced by state standardized tests. Federal funding formulas required states to decrease achievement gaps, increase graduation rates, and prepare students for careers or college. The March 2010 draft Reauthorization of the ESEA stated teachers believe colleague collaboration is imperative to improve student achievement (USDE, 2010, p. 5), and Daniels (2009) testified that school structures must exist for teachers/administrators to analyze data and set goals. Additional research noted achievement gains when teachers examined student data in Professional Learning Communities (Aylsworth, 2012; Gallagher, Means, & Padilla, 2008; Galligan, 2011; Goddard, Hoy, & Hoy, 2000; Roberts, 2010). This research developed an instrument to discern CFIP's use in improving instruction and learning. The research design was a mixed methods concurrent design using survey research with quantitative analysis and open-ended qualitative questions, and qualitative structured, teacher interviews. Participants included 81 teachers from four CFIP and four non-CFIP elementary schools in two U.S.A. Mid-Atlantic suburban school systems. Conclusions indicated that value exists in pursuing research to discern if teacher teams using data literacy methods consistently and frequently can improve classroom instruction and student learning. Surveyed teachers identified the need and desire for more time for team data analysis and data literacy coaching. Educational leaders must consider providing this time and training for all teacher teams.
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Tsivos, Zoe-Lydia. "An exploration of Compassion Focused Imagery (CFI) in women with sub-clinical eating disorder symptoms." Thesis, University of Manchester, 2015. https://www.research.manchester.ac.uk/portal/en/theses/an-exploration-of-compassion-focused-imagery-cfi-in-women-with-subclinical-eating-disorder-symptoms(04ad26e2-e555-4c72-a028-f4bf9cacae7f).html.
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Compassion Focused Therapy (CFT) is an emerging transdiagnostic treatment which aims to attenuate common psychological problems including shame and self-criticism through building compassionate skills. These problems are considered relevant and implicated in the maintenance of eating disorders. Imagery in particular has attracted attention as an important treatment technique for its strong links with memory. The aim of this thesis was to explore the effect of Compassion Focused Imagery (CFI) in women with sub-clinical eating disorder symptoms. Paper 1 is a systematic review of the CFT literature across disorders and in transdiagnostic groups. The main aims of the review were to evaluate the literature, synthesise the content of CFT across studies and provide preliminary effect size calculations. Eighteen articles were identified including clinical and non-clinical, treatment and experimental studies. The current body of evidence includes transdiagnostic samples, eating disorders, psychosis, acquired brain injury, depression, posttraumatic stress disorder (PTSD), personality disorder, individuals high in self-criticism and those with acne. The review includes a synthesis of intervention content, modalities, training and supervision of CFT within the literature. Strongest evidence was identified within transdiagnostic groups with more limited evidence for CFT within PTSD, personality disorders, acne sufferers and high self-critics. Effect sizes varied from 0-0.9 across shame, self-criticism and self-compassion outcomes. The area is limited by few controlled evaluations and heterogeneous content of interventions. Paper 2 is an experimental study of the effect on CFI in an analogue (sub-clinical) sample of women with elevated levels of eating disorder symptoms. Following baseline assessments measuring shame, self-criticism, self-compassion and stress, anxiety and depression, women with global scores of 2.5 or over on the Eating Disorders Examination Questionnaire (EDE-Q) were randomly allocated to either CFI condition in which they were instructed to create an image of a compassionate other or to a neutral imagery condition which involved creating an image of a neutral object. A third group of individuals with global scores of 1.0 or less on the EDE-Q were recruited for comparison only. Following a practice period of five-to-seven days, participants were assessed a final time. Controlling for baseline scores, significantly greater reductions in shame were reported in the CFI group compared with the neutral imagery group. Increases in self-compassion and decreases in self-criticism and depression, anxiety and stress were favourable in the CFI group compared with those in the neutral imagery group; however, these findings did not reach statistical significance. Compassion focused imagery appeared to be well tolerated within the CFI group. Clinical and theoretical implications and future research directions are discussed. The third chapter offers a reflective discussion on the methodological strengths and weaknesses, clinical and theoretical implications beginning with the systematic review followed by the empirical paper. Chapter 3 concludes with a final section on personal and professional reflections throughout the research process.
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Streit, Fernanda. "Influence des conditions de recolte et de concentration sur l'etat physiologique et la cryotolerance de lactobacillus delbrueckii subsp. bulgaricus cfl1." Phd thesis, AgroParisTech, 2008. http://pastel.archives-ouvertes.fr/pastel-00004299.
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Ce travail de thèse vise l'étude des effets des étapes de récolte et de concentration des cellules, sur la dégradation des fonctionnalités de Lactobacillus delbrueckii subsp. bulgaricus CFL1. Cet objectif s'accompagne de l'identification des mécanismes physiologiques qui expliquent les différences observées. La mise en œuvre de conditions de stress modéré, permettant aux cellules de s'adapter au stress ultérieur de congélation, constitue le troisième volet abordé dans cette thèse. Lors de la première partie du travail, l'effet d'une acidification en fin de fermentation sur la cryotolérance des cellules a été analysé. Un plan d'expériences a permis de définir la condition optimale d'acidification (pH 5,25 pendant 30 min) induisant une adaptation des cellules. Elle conduit à une meilleure résistance à la congélation et au stockage à -20 °C. Deux mécanismes physiologiques à l'origine de cette adaptation ont été identifiés. Le premier est lié à l'augmentation de la concentration de l'acide gras membranaire C18:1. Le deuxième, caractérisé pour l'analyse du protéome cytoplasmique, correspond à une réduction du métabolisme azoté, une augmentation des métabolismes énergétique et nucléotidique, et à une synthèse plus importante de protéines de stress. Dans une deuxième étape, les cellules ont été concentrées selon différentes conditions de centrifugation (vitesse de rotation, durée et température). Celles-ci n'ont pas d'effet sur la résistance de Lb. bulgaricus CFL1 à l'étape de concentration elle-même, mais présentent un effet faible mais significatif sur leur cryotolérance. Les évolutions de la résistance des cellules à la congélation et au stockage étant opposés, l'effet des conditions de centrifugation sur les cellules stockées à long terme est cependant négligeable. La troisième partie de ce travail a permis de quantifier l'impact de la concentration des cellules par microfiltration, sur leur résistance aux différentes étapes du procédé et sur l'état physiologique de Lb. bulgaricus CFL1. Les résultats montrent que les cellules sont moins résistantes à l'étape de microfiltration qu'à la centrifugation. Par contre, la résistance à la congélation augmente significativement (entre 28 % et 88 %) selon les conditions de microfiltration appliquées, par rapport à la centrifugation. La meilleure cryotolérance a été obtenue pour une vitesse tangentielle égale à 2,01 m.s-1 et une pression transmembranaire de 0,15 MPa. Cette meilleure résistance est liée à une augmentation des teneurs en acides gras C16:0, 18:1 et cycC19:0, ainsi qu'à une réduction de la concentration en C14:0. Les résultats des analyses protéomiques montrent aussi que les cellules les plus actives et les plus résistantes voient leur métabolisme énergétique augmenter, alors que leur métabolisme général et azoté est, au contraire, réduit. Finalement, ce travail propose deux voies intéressantes pour l'adaptation de Lb. bulgaricus CFL1, en vue d'améliorer sa cryotolérance. Certaines réponses physiologiques générales induisant ces adaptations cellulaires ont pu être identifiées, aux niveaux membranaire et cytoplasmique.
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Streit, Fernanda. "Influence des conditions de recolte et de concentration sur l’etat physiologique et la cryotolerance de lactobacillus delbrueckii subsp. Bulgaricus cfl1." Paris, AgroParisTech, 2008. http://www.theses.fr/2008AGPTA010.
Full textAbstract:
Ce travail de thèse vise l’étude des effets des étapes de récolte et de concentration des cellules, sur la dégradation des fonctionnalités de Lactobacillus delbrueckii subsp. Bulgaricus CFL1. Cet objectif s’accompagne de l’identification des mécanismes physiologiques qui expliquent les différences observées. La mise en œuvre de conditions de stress modéré, permettant aux cellules de s’adapter au stress ultérieur de congélation, constitue le troisième volet abordé dans cette thèse. Lors de la première partie du travail, l’effet d’une acidification en fin de fermentation sur la cryotolérance des cellules a été analysé. Un plan d’expériences a permis de définir la condition optimale d’acidification (pH 5,25 pendant 30 min) induisant une adaptation des cellules. Elle conduit à une meilleure résistance à la congélation et au stockage à -20 °C. Deux mécanismes physiologiques à l’origine de cette adaptation ont été identifiés. Le premier est lié à l’augmentation de la concentration de l’acide gras membranaire C18:1. Le deuxième, caractérisé pour l’analyse du protéome cytoplasmique, correspond à une réduction du métabolisme azoté, une augmentation des métabolismes énergétique et nucléotidique, et à une synthèse plus importante de protéines de stress. Dans une deuxième étape, les cellules ont été concentrées selon différentes conditions de centrifugation (vitesse de rotation, durée et température). Celles-ci n’ont pas d’effet sur la résistance de Lb. Bulgaricus CFL1 à l’étape de concentration elle-même, mais présentent un effet faible mais significatif sur leur cryotolérance. Les évolutions de la résistance des cellules à la congélation et au stockage étant opposés, l’effet des conditions de centrifugation sur les cellules stockées à long terme est cependant négligeable. La troisième partie de ce travail a permis de quantifier l’impact de la concentration des cellules par microfiltration, sur leur résistance aux différentes étapes du procédé et sur l’état physiologique de Lb. Bulgaricus CFL1. Les résultats montrent que les cellules sont moins résistantes à l’étape de microfiltration qu’à la centrifugation. Par contre, la résistance à la congélation augmente significativement (entre 28 % et 88 %) selon les conditions de microfiltration appliquées, par rapport à la centrifugation. La meilleure cryotolérance a été obtenue pour une vitesse tangentielle égale à 2,01 m. S-1 et une pression transmembranaire de 0,15 MPa. Cette meilleure résistance est liée à une augmentation des teneurs en acides gras C16:0, 18:1 et cycC19:0, ainsi qu’à une réduction de la concentration en C14:0. Les résultats des analyses protéomiques montrent aussi que les cellules les plus actives et les plus résistantes voient leur métabolisme énergétique augmenter, alors que leur métabolisme général et azoté est, au contraire, réduit. Finalement, ce travail propose deux voies intéressantes pour l’adaptation de Lb. Bulgaricus CFL1, en vue d’améliorer sa cryotolérance. Certaines réponses physiologiques générales induisant ces adaptations cellulaires ont pu être identifiées, aux niveaux membranaire et cytoplasmique.
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Romo, Medina María Alejandra. "Efecto de metformina sobre la viabilidad de esferas derivadas de células de carcinoma mamario canino (Canis lupus familiaris) CF41.Mg." Tesis, Universidad de Chile, 2016. http://repositorio.uchile.cl/handle/2250/141837.
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Memoria para optar al Título Profesional de Médico VeterinarioUna de las enfermedades más frecuentes en la hembra canina es la neoplasia mama-ria. Los tratamientos más utilizados son la cirugía y la quimioterapia adyuvante, las cuales en muchas ocasiones pueden resultar insuficientes debido probablemente a la existencia dentro del tumor de un subgrupo de células llamadas células neoplásicas iniciadoras de tumores (CNT), que presentan características de troncalidad favoreciendo la reaparición tumoral y metástasis. Estas células exhiben quimioresistencia, por lo cual es fundamental buscar nuevas alternativas citotóxicas para este tipo de células. Metformina, droga utilizada en humanos para el tratamiento de la diabetes mellitus tipo II, ha demostrado modular la actividad proliferativa de CNT, no obstante en caninos hay muy pocos datos pertinentes. En este estudio se evaluó el efecto de metformina sobre la viabilidad y capacidad de formación de esferas celulares (estructuras ricas en CNT) derivadas de células de carcino-ma mamario canino CF41.Mg. Metformina indujo una disminución tanto del número como del tamaño de esferas celulares a 10 y 20 mM (p<0,0001). Sin embargo, la droga no gatilló reducción en la viabilidad de estas células a las 48 horas de incubación respecto al grupo control. Por otro lado, en células CF41.Mg parentales, la viabilidad en presencia de la droga (10 y 20 mM por 48 horas) disminuyó (p<0,0001), lo cual implica que estas células presen-tan mayor sensibilidad a la metformina en comparación con las esferas celulares. Estos resultados sugieren que metformina ejercería actividad citotóxica en esferas derivadas de células de carcinoma mamario canino CF41.Mg, siendo este efecto dependien-te del tiempo de exposición a la droga. Metformina podría ser considerada como una droga que afecta a CNT, lo cual sustenta futuros estudios para esclarecer el potencial rol terapéu-tico de esta droga en la neoplasia mamaria canina.
Mammary neoplasia is one of the most frequent diseases in female dogs. The most common treatment used is surgery and adyuvant chemotherapy, which may often be insuf-ficient due to the existence of a subset of cells called tumor initiating neoplastic cells (CSC) within the tumor, that exhibit stemness characteristics, favoring tumor relapse and metastasis. These cells display chemoresistance, which supports the search for new citotox-ic alternatives. Metformin, a drug used in human diabetes mellitus type II treatment, modu-lates the proliferative activity on CSC, however there are scarce data about this effect in dogs. In this study, the effect of metformin on cell viability and spheres forming ability (structures rich in CSC) derived from canine mammary carcinoma cells CF41.Mg were evaluated. Metformin induced a decrease in number and sized of spheres at 10 and 20 mM (p<0.0001). However, the drug did not reduce viability of these cells at 48 hours of incuba-tion respect to the control group. On the other hand, viability on CF41.Mg parental cells in presence of the drug (10 and 20 mM for 48 hours) decreased, which implies greater sensi-tivity to metformin in relation to spheres. These results suggest that metformin exert cytotoxic activity in spheres derived from CF41.Mg canine mammary carcinoma cells. These effects are dependent on the expo-sure time to the drug. Metformin may be considered a drug that affects CSC, which sup-ports future studies to clarify its potential therapeutic role in canine mammary neoplasia.
Financiamiento: Proyecto Fondecyt 11110148.
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Brown, Nicholas. "Control-flow Integrity for Real-time Embedded Systems." Digital WPI, 2017. https://digitalcommons.wpi.edu/etd-theses/402.
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As embedded systems become more connected and more ubiquitous in mission- and safety-critical systems, embedded devices have become a high- value target for hackers and security researchers. Attacks on real-time embedded systems software can put lives in danger and put our critical infrastructure at risk. Despite this, security techniques for embedded systems have not been widely studied. Many existing software security techniques for general purpose computers rely on assumptions that do not hold in the embedded case. This thesis focuses on one such technique, control-flow integrity (CFI), that has been vetted as an effective countermeasure against control-flow hijacking attacks on general purpose computing systems. Without the process isolation and fine-grained memory protections provided by a general purpose computer with a rich operating system, CFI cannot provide any security guarantees. This thesis explores a way to use CFI on ARM Cortex-R devices running minimal real-time operating systems. We provide techniques for protecting runtime structures, isolating processes, and instrumenting compiled ARM binaries with CFI protection.
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Sinclair, Hugh D. "The North Helvetic Flysch of eastern Switzerland : Foreland Basin architecture and modelling." Thesis, University of Oxford, 1989. http://ora.ox.ac.uk/objects/uuid:0e83a6d2-cf51-4dd3-b4bb-523a1d28fc90.
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The North Alpine Foreland Basin (NAFB) comprises sediments of late Eocene to middle Miocene age. The earliest deposits are the North Helvetic Flysch which are exposed in the regions of Glarus and Graubunden, eastern Switzerland. The Taveyannaz sandstones are the first thrust wedge (southerly) derived sediments of the North Helvetic Flysch. The Taveyannaz basin was divided into two sub-basins by a thrust ramp palaeohigh running ENE/WSW (parallel to the thrust front). Palaeocurrent directions were trench parallel towards the ENE. Sedimentation in the Inner basin (140m thick) is characterised by very thick bedded turbidite sands generated by thrust induced seismic events confined within the thrust-top basin. The Outer basin (240m min. thickness) comprises 10-15 sand packages (5-100m thick) formed by turbidite sands which are commonly amalgamated. Sedimentation in the Outer basin is considered to have been controlled by thrust-induced relative sea-level variations. The Inner basin underwent intense deformation at the sediment/water interface prior to the emplacement of a mud sheet over the basin whilst the sediments were partially lithified. Later tectonic deformation involved fold and thrust structures detaching in the underlying Globigerina marls. The stratigraphy of the NAFB can be considered as two shallowing upward megasequences separated by the base Burdigalian unconformity. This stratigraphy can be simulated by computer by simplifying the foreland basin/thrust wedge system into 4 parameters: 1) the effective elastic thickness of the foreland plate, 2) a transport coefficient to describe the erosion, transport and deposition of sediment, 3) the surface slope angle of the thrust wedge, 4) the thrust wedge advance rate. The Alpine thrust wedge underwent thickening during the underplating of the External Massifs at about 24-18Ma. This event is simulated numerically by slowing the thrust wedge advance rate, and increasing the slope angle and keeping all other parameters constant. This event causes rejuvenation of the forebulge, and erosion of the underlying stratigraphy, so simulating the base Burdigalian unconformity without recourse to eustasy or anelastic rheologies to the foreland plate.
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Ball, Daniel. "Development of novel hyperpolarized magnetic resonance techniques and compounds for perfused organs." Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:21f6b661-cf21-46e7-9c7a-7c5d9ccf2b28.
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Hyperpolarization via the Dynamic Nuclear Polarization (DNP) technique has revolutionized our ability to study metabolic changes in real time. The aim of this thesis was to build upon previous work centered around the use of DNP within the isolated perfused rat heart, a well established model system for the study of cardiac metabolism, to enhance the information that can be obtained through the combination of DNP with perfused organs. Initially this was done by using the widely studied DNP probe, [1-13C]pyruvate, to generate images of metabolism within the isolated perfused rat heart. The developed technique was then successfully demonstrated in two models of myocardial infarction. The thesis then proceeds to develop an understanding of how the supra-physiological concentrations of [1-13C]pyruvate commonly used in DNP experiments can affect metabolism in the isolated perfused rat heart, and the way in which the myocardium responds to those changes if it is not adequately supplied with substrates ordinarily present in vivo, namely fatty acids. New methods of providing the heart with these required substrates were developed, without significant interference to the biochemical information acquired from DNP experiments. As [1-13C]pyruvate only provides information on a small subset of carbohydrate metabolism, the next chapter develops new compounds to be used with DNP, which would allow the exploration of short chain fatty acid metabolism (butyrate) as well as ketone body metabolism (β-hydroxybutyrate and acetoacetate), and other aspects of carbohydrate metabolism (lactate and alanine). These compounds were developed and then tested for their potential usefulness in the isolated perfused heart. Finally, as the isolated perfused rat heart lacks the diversity of genetic disease models available in the mouse, the final chapter expanded the use of DNP to the isolated perfused mouse heart with all the size challenges that this entails, and makes the transition from the heart to the liver, in order to provide an alternative metabolic viewpoint on the biochemistry of disease models. This thesis thereby permits studies involving isolated perfused organs to be carried out whilst exploiting all the tools that DNP has to offer and consequentially, allows for a vast array of physiologically derived information to help us better understand metabolic diseases.
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Aziz, Azahar. "A longitudinal study exploring post-school transitions of young people with learning disabilities : perspectives of young people, parents and professionals." Thesis, University of Dundee, 2014. https://discovery.dundee.ac.uk/en/studentTheses/c0a16519-cf61-4959-9c24-50723a31333b.
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This is a longitudinal study that aimed to explore the planning and preparation, and experiences of post-school transition of young people with learning disabilities in both, mainstream and special schools within one local authority in Scotland. Data were drawn from semi-structured interviews with the young people, parents and professionals, and also from questionnaire with parents, at three different stages, covering a period from the final year at school, up to the first year at college. In addition, observation was undertaken of two review meetings in one special school. The results found that despite all young people’s post-school destination being college, they went through a variety of experiences, suggesting that a different approach needs to be taken to planning and preparation to ensure a smooth and seamless transition. The study revealed that despite many improvements, there was still a lack of information about the post-school provisions for the young people among the school staff and other professionals. The study proposes that the role of parents is as important as the role of the school staff. The study also makes some recommendations for future research, policy and practice.
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Turvey, Keith. "Narrative ecologies : a teacher-centred model for professional learning and practice with technologies in initial teacher education." Thesis, University of Brighton, 2011. https://research.brighton.ac.uk/en/studentTheses/f2fd22b1-cfb1-4c22-ab78-51ab27d52e77.
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This thesis documents the evolution and evaluation of a conceptual model for developing and researching student teachers’ online pedagogical practice. The research is set against a backdrop of significant investment in web-based technologies in formal schooling in the United Kingdom (UK). Thus, the research questions some of the factors that affect the development of student teachers’ online professional and pedagogical practice faced with new opportunities to utilise web-based tools. As the project evolved it became apparent that a more fundamental research question was: How do we research student teachers’ professional learning and development with new technologies in a manner that recognises their active agency in the process?
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Hansbury, Paul. "The agency of smaller powers : Belarus in international relations." Thesis, University of Oxford, 2017. https://ora.ox.ac.uk/objects/uuid:0f9c6793-cfe1-4cbb-bdc4-c1bc6e749c6a.
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Smaller powers wield agency in the international system and they do so in many cases independently of institutions. This is problematic not only from the perspective of realism, where 'might is right,' but also from the perspective of liberalism which argues that smaller powers use institutions to obtain policy objectives. This thesis considers this problem in respect of the new states that emerged from the former Soviet Union at the end of 1991, with particular focus on Belarus which is a least-likely case for smaller power agency given its alliance with Russia and few institutional memberships. Through a detailed case study of Belarus's foreign policy, I first argue that smaller power agency has been articulated in a regional context when the smaller power withdraws its consent to the regional order. While Belarus has generally consented to Russia's regional primacy or hegemony since 1991, this consent is not unconditional, and the violation of regional interstate norms by Russia caused Belarus to make efforts to renegotiate the rules underpinning the regional order. My second, related argument is that differentiation from the regional power, Russia, proved crucial for the formulation of independent policy positions by the smaller power's officials, and, in turn, that differentiation of opinions among the smaller power's elite granted flexibility to monitor and adapt its actions (that is, allowed the smaller power to wield agency). It did so without outright provoking the regional power to intervene in the smaller power, and instead kept the regional power engaged. The findings receive tentative support from auxiliary study of other post-Soviet smaller powers, and recapitulate the merits of the neoclassical realist research agenda.
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Sutipatanasomboon, Arpaporn [Verfasser], Martin [Gutachter] Hülskamp, and Ute [Gutachter] Höcker. "Functional analysis of Arabidopsis thaliana lesion mimic mutant cfs1 in ESCRT complex-related protein trafficking / Arpaporn Sutipatanasomboon ; Gutachter: Martin Hülskamp, Ute Höcker." Köln : Universitäts- und Stadtbibliothek Köln, 2015. http://d-nb.info/1120557941/34.
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Gallmeier, Jaramillo Michelle Daniela. "Efecto antineoplásico de un extracto de Solanum Dulcamara I (BIRM®) sobre células de carcinoma mamario canino (Canis Lupus Familiaris) CF41.Mg." Tesis, Universidad de Chile, 2017. http://repositorio.uchile.cl/handle/2250/144992.
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Tesis para optar al Grado de Magíster en Ciencias Animales y Veterinarias .La neoplasia mamaria es la patología tumoral de mayor frecuencia de presentación en la hembra canina. Dado que las terapias disponibles son limitadas, no selectivas y pueden desencadenar potenciales efectos adversos, se hace necesario estudiar nuevas alternativas de tratamiento antitumoral más inocuas e idealmente selectivas. El extracto comercial derivado de la planta Solanum dulcamara L -BIRM- ha sido usado empíricamente para una amplia variedad de enfermedades, incluyendo cáncer. Sin embargo, hay escasos estudios que revelan su potencial antitumoral. BIRM induce un efecto antiproliferativo y pro-apoptótico sobre células de carcinoma de próstata humana, especialmente en células andrógeno-dependientes, donde disminuye la expresión y promueve la degradación del receptor de andrógenos (RA). Ya que sobre el 80% de las neoplasias mamarias caninas de alto grado histológico exhiben la presencia del RA, pareció interesante analizar si BIRM induce también efectos antiproliferativos sobre células de carcinoma mamario canino CF41.Mg, línea celular representativa de tumores mamarios de alto grado. Con este objeto, se estudió proliferación, capacidad clonogénica y potencial citotóxico de dichas células en presencia de BIRM. Complementariamente, se analizó el efecto del extracto sobre la habilidad de estas células para migrar e invadir. BIRM disminuyó la proliferación celular y capacidad clonogénica de las células estudiadas, efectos que fueron dependientes de la concentración. Afín a estos resultados, el extracto indujo un arresto en G0/G1. BIRM no es selectivo, dado que disminuyó también la proliferación de células no tumorales MDCK. Adicionalmente, BIRM indujo apoptosis y una disminución significativa en la migración e invasión de células CF41.Mg. Estos resultados demuestran que BIRM, induce un efecto antiproliferativo y anti-invasivo sobre células de carcinoma mamario canino y sustenta la idea que este representaría un potencial agente terapéutico para el manejo del cáncer mamario canino.
Mammary tumors are the most frequent oncologic pathology in the female dog. Since available therapies are limited, non-selective and may trigger potential adverse effects, it`s becomes necessary to study new, harmless and ideally selective antitumor treatment alternatives. Commercial herbal extract derived from Solanum DulcamaraL -BIRM- has been used empirically for a wide variety of diseases, including cancer. However, there are few studies that reveal its antitumor potential. BIRM induces an antiproliferative and pro-apoptotic effect on human prostate carcinoma cells, especially in androgen-dependent cells, where it decreases expression and promotes androgen receptor (AR) degradation. Since over 80% of high histological grade canine mammary tumors exhibit the presence of RA, it appeared interesting to analyze whether BIRM also induces antiproliferative effects on CF41.Mg canine mammary carcinoma cells, a cell line representative of high grade mammary tumors. For this purpose, proliferation, clonogenic capacity and potential cytotoxicity were studied in the presence of BIRM. In addition, the effect of the extract on the ability of CF41.Mg cells to migrate and invade was analyzed. BIRM decreased cell proliferation and clonogenic ability of the cells studied, effects that were concentration-dependent. In parallel, the extract induced an arrest in G0/G1. BIRM is not selective, since the proliferation of non-tumoral MDCK cells also decreased in presence of herbal extract. Moreover, BIRM induced apoptosis and a significant decrease in the migration and invasion of CF41.Mg. cells. These results demonstrate that BIRM induces an antiproliferative and anti-invasive effect on canine mammary carcinoma cells and, supports the idea that it would represent a potential new therapeutic agent for the management of canine mammary cancer.
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Collard, Bruno. "Etude d'antennes sur aéronef ou en environnement aéroportuaire par hybridation de méthodes de résolution des équations de Maxwell." Toulouse 3, 2007. http://www.theses.fr/2007TOU30080.
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Hybridation d'une méthode intégrale (MoM) avec une méthode de rayon (UTD), en particulier en présence de corps imbriqués. C'est le point de départ d'une réflexion synthétique plus générale sur les méthodes de raccordement utilisées en électromagnétisme qui conduit à une présentation unifiée de ces raccordements au travers d'une classification en deux types dont les conditions de non singularité sont démontrées. Cette présentation est applicable à tous les types de formulation des champs : différentielle, intégrale, asymptotique, spectrale… Outre l'hybridation, deux autres applications de cette approche sont proposées : une nouvelle formulation intégrale bien posée sur corps impédant prolongeable aux objets parfaitement conducteurs et la formulation CFIE sur corps mince ou ouvertImplementation of a hybrid Integral Equation Method (MOM)/ Uniform Theory of Diffraction (UTD) formulation for small dielectric (or PEC) protrusions embedded into a large conducting body. Carrying out hybrid techniques leads to more general considering about connection of electromagnetic problems. Resulting from that, a reliable interpretation and a unified classification of usual connection techniques into two types is proposed. For both type, well posedness conditions are pointed out. This classification applies to all types of fields formulations (differential, integral, ray, spectral…). In addition to Hybrid MoM/UTD, two more applications of this lateral thinking are proposed: a new well posed integral formulation for scattering by impedant (IBC) 3D bodies that can be extended to PEC and the formulation of CFIE on an open or thin PEC object
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Gray, Robert A. "Inflight detection of errors for enhanced aircraft flight safety and vertical accuracy improvement using digital terrain elevation data with an inertial navigation system, global positioning system and radar altimeter." Ohio University / OhioLINK, 1999. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1181161513.
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Rossée, Sebastian [Verfasser]. "Hämodynamisches Monitoring während akuter Koronarischämie : Eine vergleichende Untersuchung der PiCCO-Variablen CFI, GEF und dP/dtMax mit der transösophagealen Echokardiografie am experimentellen Schweinemodell / Sebastian Rossée." Kiel : Universitätsbibliothek Kiel, 2011. http://d-nb.info/1020245301/34.
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Jagannathan, Ramanujan. "Evaluation of Crossover Displaced Left-turn (XDL) Intersections and Real-time Signal Control Strategies with Artificial Intelligence Techniques." Thesis, Virginia Tech, 2003. http://hdl.handle.net/10919/10144.
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Although concepts of the XDL intersection or CFI (Continuous Flow Intersection) have been around for approximately four decades, users do not yet have a simplified procedure to evaluate its traffic performance and compare it with a conventional intersection. Several studies have shown qualitative and quantitative benefits of the XDL intersection without providing accessible tools for traffic engineers and planners to estimate average control delays, and queues. Modeling was conducted on typical geometries over a wide distribution of traffic flow conditions for three different design configurations or cases using VISSIM simulations with pre-timed signal settings. Some comparisons with similar conventional designs show considerable savings in average control delay, and average queue length and increase in intersection capacity. The statistical models provide an accessible tool for a practitioner to assess average delay and average queue length for three types of XDL intersections. Pre-timed signal controller settings are provided for each of the five intersections of the XDL network.
In this research, a "real-time" traffic signal control strategy is developed using genetic algorithms and neural networks to provide near-optimal traffic performance for XDL intersections. Knowing the traffic arrival pattern at an intersection in advance, it is possible to come up with the best signal control strategy for the respective scenario. Hypothetical cases of traffic arrival patterns are generated and genetic algorithms are used to come up with near-optimal signal control strategy for the respective cases. The neural network controller is then trained and tested using pairs of hypothetical traffic scenarios and corresponding signal control strategies. The developed neural network controller produces near-optimal traffic signal control strategy in "real-time" for all varieties of traffic arrival patterns.Master of Science
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Vice, President Research Office of the. "Seismic Shift." Office of the Vice President Research, The University of British Columbia, 2006. http://hdl.handle.net/2429/2662.
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Strachan, Olivean. "The Impact of a Multifaceted Intervention on student Math and ELA Achievement." ScholarWorks, 2015. https://scholarworks.waldenu.edu/dissertations/333.
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Closing the achievement gaps in mathematics and English language arts (ELA) is an ongoing challenge for most New York City Public school administrators. One New York school experiencing this problem implemented a broad intervention including (a) the Children First Intensive (CFI) program, which includes using data to inform instructional and organizational decision-making; (b) added baseline and post assessments; and (c) differentiated instruction including student conferences. The effects of the intervention had not been evaluated within the context of implementation. The purpose of this quantitative study was to evaluate the impact of the multifaceted learning gaps' intervention on 6th grade student achievement in math and ELA. The framework used in this study was the Halverson, Grigg, Prichett, and Thomas data-driven instructional systems model. The comparative study design used paired t tests to examine the change in math and ELA achievement scores on a group of 6th grade students (N = 26), before after the intervention. Results indicated significant increases in the test scores of the students, suggesting that students' learning gaps were closed using their assessment results and differentiated instruction within the comprehensive intervention. Results were used to create a professional development handbook on using a multifaceted data-based approach to improve student achievement. Positive social change might occur by providing the local site findings on the outcomes of their approach and additional training on using the approach, which may ultimately improve the academic performance of all students.
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Hons, Petr. "Rozšíření generického ladicího nástroje v projektu Lissom." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2014. http://www.nusl.cz/ntk/nusl-236020.
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This thesis deals with an introduction to debugging and debuggers. The thesis describes principles of the debugging information, especially the DWARF format and its Call Frame Information (CFI), that enables a debugger to visualize the call stack. Furthermore, extensions of the debugger used in the Lissom project were designed and implemented. These extensions added support for call stack visualization, history value storage and step return and step over commands.
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Blakeslee, Amber. "Higher Education Financial Health - A Case Study of the California State University (CSU)." CSUSB ScholarWorks, 2019. https://scholarworks.lib.csusb.edu/etd/909.
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Higher education is in a challenging financial time. Overall, states are investing considerably less in higher education than they did a decade ago and students are paying significantly more in tuition and fees. Simultaneously, the higher education landscape is changing – changing in terms of demographics, modes of delivery, workforce needs, funding and cost structures, and perceptions of value. Almost every day there is a new media story about a college or university experiencing financial difficulties. With decreasing confidence from campus financial officers in the long-term sustainability of their institutions and campus closures expected to escalate in the coming years, there is a significant need to better understand higher education financial health so that colleges and universities can proactively address challenges as they arise.
Research pertaining to higher education financial health, particularly with respect to public higher education, was found to be limited. This project, first explored the research and methods in use to measure higher education financial health. Then, utilizing the Composite Financial Index (CFI), the most widely adopted metric for measuring financial health identified during the literature review, addressed a research gap related to financial analysis in public higher education through conducting a quantitative analysis of the California State University (CSU) system. The CSU, the largest four-year public higher education system in the country, serves as an important litmus test for the higher education industry as a whole given its sheer magnitude in educating over 480,000 students each year and producing one out of every ten workers in California. In addition, leading indicators signal that California public higher education should be exceeding industry performance given that California is the 5th largest economy in the world during a lengthy period of economic growth, is one of only four states to invest more in higher education in 2018 than it did in 2008, and has the 7th highest tuition rate increases over the same time period.
The quantitative analysis of the CSU consisted of a four-pronged approach: 1) Analyze system financial health over a 20 year period; 2) analyze campus financial health over a five-year period; 3) analyze the CSU’s CFI over a 20 year period in comparison to key variables - Gross Domestic Product (GDP) growth rate trends, CSU state funding changes, and CSU tuition rate changes; and 4) analyze campus CFIs with campus enrollment size.
Overall, results indicate significant underlying financial concerns for the CSU and disaggregating the results by campus indicate even greater financial concerns at a campus level, reinforcing the notion that smaller campuses experience disparate financial impacts and are more susceptible to closure if left unaddressed. In addition, this research establishes correlations with key variables analyzed and outlines recommendations for future research to further validate findings and more closely identify causality. These findings reinforce the need for colleges and universities to develop a sense of urgency to proactively address the changes and challenges that are occurring, with greater use of strategic financial analysis needed to achieve transformation.
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Buy-Arnould, Clotilde. "Etude par résonance paramagnétique électronique en modes continu et pulsé des sites métalliques des ATPAses CF1 de chloroplastes d'épinard, Spinacia Oleracea, et TF1 de la bactérie thermophile Bacillus PS3." Châtenay-Malabry, Ecole centrale de Paris, 1996. http://www.theses.fr/1996ECAP0497.
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Ce travail présente les résultats d'études spectroscopiques sur les ATPases CF1 de chloroplastes d'épinard (Spinacia Oleracea) et TF1 de la bactérie thermophile Bacillus PS3. Les cations métalliques divalents tels Ca2+ ou Mg2+ jouent un rôle fondamental dans l'activité catalytique de ces enzymes, mais leur mode d'action exact n'est pas encore élucidé. Nous avons élaboré une méthode de substitution par des cations actifs en Résonance Paramagnétique Electronique (RPE), afin d'utiliser cette technique comme méthode d'investigation de leurs sites de fixation. Par RPE en modes continu et pulsé, nous avons identifié certains ligands de Mn2+ dans le site de plus forte affinité M1 de CF1. Le cation dans ce site est en interaction avec l'ADP fortement lie du CF1, ce qui démontre la proximité de ces sites métallique et nucléotidique. Pour le TF1, qui est purifié sans nucléotide endogène, une telle interaction avec un groupement phosphate n'est pas détectée. En revanche, nous montrons qu'un groupement azote, certainement l'amine terminale d'une lysine (peut-être la ß -Lys-164), est ligand de Mn2+ dans le site M1. Lorsque les sites M2 et M3 de CF1 sont occupés par des cations, nous détectons une interaction entre Mn2+ et une amine primaire provenant probablement d'une lysine. Les données spectroscopiques obtenues avec VO2+ fixe dans le site de plus forte affinité M1 de TF1 confirment l'existence d'une coordination du cation par l'amine terminale d'une lysine. En outre, le groupement imidazole d'une histidine est également ligand de VO2+. Nous avons mis en évidence la formation d'un complexe ternaire VO. TF1. ATP, lors de l'addition simultanée de VO2+ et d'ATP à l'échantillon de protéine. L'emploi de HYSCORE, technique de RPE pulsée en 2 dimensions, a aussi permis une progression significative dans l'interprétation des spectres à une dimension.
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Harris, Jenine, Leslie Hinyard, Kate E. Beatty, Jared B. Hawkins, Elaine O. Nsoesie, Raed Mansour, and John S. Brownstein. "Evaluating the Implementation of a Twitter-Based Foodborne Illness Reporting Tool in the City of St. Louis Department of Health." Digital Commons @ East Tennessee State University, 2018. https://dc.etsu.edu/etsu-works/6821.
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Foodborne illness is a serious and preventable public health problem affecting 1 in 6 Americans with cost estimates over $50 billion annually. Local health departments license and inspect restaurants to ensure food safety and respond to reports of suspected foodborne illness. The City of St. Louis Department of Health adopted the HealthMap Foodborne Dashboard (Dashboard), a tool that monitors Twitter for tweets about food poisoning in a geographic area and allows the health department to respond. We evaluated the implementation by interviewing employees of the City of St. Louis Department of Health involved in food safety. We interviewed epidemiologists, environmental health specialists, health services specialists, food inspectors, and public information officers. Participants viewed engaging innovation participants and executing the innovation as challenges while they felt the Dashboard had relative advantage over existing reporting methods and was not complex once in place. This study is the first to examine practitioner perceptions of the implementation of a new technology in a local health department. Similar implementation projects should focus more on process by developing clear and comprehensive plans to educate and involve stakeholders prior to implementation.
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Sprecher, Jürg. "Mit Machtverzicht zum Einheitsstaat eine politökonomische Analyse zum institutionellen Wandel in der Schweiz." Zürich Chur Rüegger, 2009. http://www.rueggerverlag.ch/page/verzeichnis/detail.cfm?CFID=208224592&CFTOKEN=cf4bacd212e03bb1-E0C619AD-091F-13C5-17CF98517B978245&id=717.
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Peng, Kaidi. "Impact of pulsed electric fields (PEF) and ultrasound (US) on the growth and conservation of lactic acid bacteria." Thesis, Compiègne, 2021. http://www.theses.fr/2021COMP2614.
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Ce travail vise à étudier les effets du traitement par champs électriques pulsés (CEP) et par ultrasons (US) sur les cinétiques de croissance et d’acidification des bactéries lactiques, ainsi que sur la viabilité cellulaire après congélation et lyophilisation. Les effets de diverses conditions opératoires (e.g., durée et intensité du traitement, température de congélation, vitesse de congélation, température de stockage, présence ou non de cryoprotecteurs, prétraitement ou non par CEP, etc) ont été étudiés. La bactérie lactique Lactobacillus delbrueckii subsp. bulgaricus CFL1 a été utilisée comme microorganisme modèle dans cette étude. La première partie de ce travail a été consacrée à l’étude des effets de traitement par CEP ou par US sur les cinétiques de croissance et d’acidification de L. bulgaricus CFL1 durant la fermentation sur un milieu MRS. Le traitement par CEP et US pendant la fermentation a entraîné un retard de la croissance cellulaire et une diminution de l'activité d'acidification. Après l'arrêt du traitement, les cellules traitées ont retrouvé une croissance normale, montrant un taux de croissance et d'acidification accéléré par rapport aux cellules non traitées (contrôle). Les cellules ont présenté une croissance biphasique au cours de la fermentation assistée par CEP et assistée par US. La seconde partie du travail a été consacrée à l’étude des conditions opératoires sur la viabilité de L. bulgaricus CFL1 après congélation et lyophilisation. Les résultats ont montré que l'application d'un protocole de congélation approprié et la réduction du soluté extracellulaire (NaCl) sont importants pour maximiser la viabilité cellulaire après congélation. La viabilité la plus élevée était obtenue quand la conservation cellulaire a été effectuée à -80 °C. Les cellules en suspension dans 10% de saccharose avaient une viabilité après lyophilisation plus élevée que celles qui étaient en suspension dans l'eau ultrapure. Lorsque les cellules étaient suspendues dans une solution de saccharose ou une solution de glycérol, puis congelées à -80 °C, elles avaient une viabilité plus élevée que celles qui étaient congelées à -20 °C. Le traitement par CEP n'a montré aucun effet positif sur la viabilité cellulaire après congélation ou lyophilisationThis work aims to investigate the effects of pulsed electric fields (PEF) and ultrasound (US) treatments on the growth and acidification kinetics of lactic acid bacteria (LAB), as well as the impact of processing conditions (e.g., freezing medium, freezing kinetics, storage temperature, cryoprotectants, PEF treatment) on the viability of LAB after freezing and freeze-drying. In this work, Lactobacillus delbrueckii subsp. bulgaricus CFL1 was used as a model culture. The first part of this work deals with the effects of PEF and US treatments on the growth and acidification kinetics of L. bulgaricus CFL1 cells during fermentation in MRS medium. The application of PEF and US treatments during fermentation resulted in a delayed growth and a reduced acidification activity of the cells. After suspending the treatment, the treated-cells showed an accelerated growth and acidification rate, and reached at the end of fermentation the same values obtained for to untreated cells (control). It was also demonstrated that L. bulgaricus CFL1 cells exhibited a biphasic growth during PEF-assisted and US-assisted fermentation. The second part of this study was devoted to investigate the influence of processing conditions on the viability of L. bulgaricus CFL1 cells after freezing and freeze-drying. Applying appropriate freezing protocols and reducing the extracellular solute (NaCl) were important to obtain high viability after freezing. The cells had higher viability during storage at -80 °C compared to that at -20 °C. The cells suspended in 10% sucrose had higher viability than those suspended in ultrapure water after freeze-drying. When the cells were suspended in ultrapure water, and in presence of sucrose or glycerol as cryoprotectants, they had higher viability both after freezing and freeze-drying when they were frozen at -80 °C than that at -20 °C. PEF treatment had no positive effect on the viability of cells after freezing or freeze-drying
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Moreno, Morcillo Maria. "Etude structurale du complexe CstF et de son homologue chez la levure CF IA, deux facteurs indispensables pour la maturation 3' des pré-ARN messagers." Thesis, Bordeaux 2, 2010. http://www.theses.fr/2010BOR21719/document.
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Une étape clé dans la maturation des pré-ARNms est le clivage et la polyadénylation que ceux-ci subissent sur leur extrémité 3’. Chez les métazoaires, le complexe CstF (Cleavage stimulation Factor) reconnaît une région de l’ARNm riche en U et U/G et stabilise le complexe CPSF (Cleavage Polyadenylation Stimulating Factor) sur le site de polyadénylation. Nous avons déterminé la structure cristallographique du domaine N-terminal d’une des trois sous-unités de CstF, CstF-50. Ce domaine forme un homodimère compact et présente deux surfaces identiques conservées dérivées de la formation du dimère. La structure dimérique de CstF-50 est en accord avec le modèle hexamèrique du complexe. L’homologue de CstF chez la levure, CF IA (Cleavage/polyadenylation Factor IA), est impliqué dans les réactions de clivage et polyadénylation de la maturation 3’. Nous avons reconstitué le complexe entier ‘in vitro’ et résolu la structure en solution par RMN des régions minimales impliquées dans l’interaction des sous-unités Rna14p et Rna15p. Pour la formation de l’hétérodimère, la région C-terminale de Rna14p, que nous avons appelé domaine « monkeytail », s’entrelace intimement avec la région « hinge » de Rna15p. La présence de ces deux domaines chez leurs homologues de mammifères, CstF-77 et CstF-64, suggère la conservation de ce type d’organisation entre ces deux sous-unités à travers les espècesThe removal of the 3’ region of pre-mRNA followed by polyadenylation is a key step in mRNA maturation. In metazoa, Cleavage stimulation Factor (CstF) recognizes U and G/U rich cis-acting RNA sequence elements through its 64kDa subunit and helps stabilize the Cleavage Polyadenylation Stimulating Factor (CPSF) complex at the polyadenylation site. We describe the crystal structure of the N-terminal domain of the CstF-50 subunit. Through highly conserved residues, CstF-50 forms a compact homodimer that exposes two geometrically opposite and identical conserved surfaces. Together with prior data, the structure of the CstF-50 homodimerization domain supports a hexameric model of CstF. The yeast homologue of CstF is the Cleavage/polyadenylation Factor IA (CF IA) complex and is involved in both the cleavage and polyadenylation of pre-mRNA. We have reconstituted ‘in vitro’ the overall complex and also solved the solution structure of one of the inter-subunit regions, specifically the heterodimer involving peptides from Rna14p and Rna15p. Upon binding, a short C-terminal region from Rna14p wraps intimately within the central hinge domain from Rna15p. Conservation of residues reveals that the structural tethering is preserved in the homologous mammalian proteins
La maduración 3’ del pre-ARNm es un proceso clave de la expresión génica que incluye el corte y la poliadenilación del extremo 3’ libre del pre-ARNm. En metazoos, el complejo CstF (Cleavage stimulation Factor) reconoce una secuencia del pre-ARNm rica en U y G/U y permite la estabilización del complejo CPSF (Cleavage Polyadenylation Stimulating Factor) en el sitio de poliadenilación. Hemos descrito la estructura cristalina del dominio N-terminal de una de las tres subunidades de CstF, CstF-50. La estructura ha revelado la organización de la proteína en un dímero compacto y conservado entre las especies. Dos zonas idénticas conservadas se encuentran expuestas a ambos lados de la superficie estructural. Nuestros resultados corroboran así la hipótesis sobre el modelo hexamérico del complejo CstF. CF IA (Cleavage/ polyadenylation Factor IA), el homólogo de CstF en levaduras, interviene en las dos etapas de la maduración 3’. Las bases para la reconstitución del factor CF IA ‘in vitro’ han sido establecidas. Al mismo tiempo, hemos resuelto la estructura del subcomplejo formado por las regiones de interacción de Rna14p y de Rna15p en solución mediante RMN. En el heterodímero, las dos proteínas forman una entidad única a través de la región C-terminal de Rna14p, dominio “monkeytail”, y el dominio “hinge” de Rna15p, quedando las hélices de la dos proteínas entrelazadas. La localización de estos dominios en sus homólogos mamíferos, CstF-77 et CstF-64, sugiere que este tipo de organización está conservada entre las especies
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Leal, Matheus Hermes. "Cofilina-1 (CFL-1) correlaciona-se à sobrevida mediana em pacientes com carcinoma de pulmão não de pequenas células tratados com radioterapia." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2016. http://hdl.handle.net/10183/158177.
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Base teórica: O câncer de pulmão é uma doença com alta incidência e mortalidade, cujo prognóstico permanece discreto apesar do melhor entendimento da doença nas últimas décadas. A radioterapia tem papel terapêutico em todos os estágios da doença. A expressão da cofilina-1, uma proteína relacionada à mobilidade celular, determinou maior radiossensibilidade a células de adenocarcinoma brônquico em estudos in vitro, porém pior sobrevida em estádios iniciais Objetivo: Avaliar se a expressão da cofilina-1 interfere na sobrevida e no controle local em pacientes com câncer de pulmão submetidos a tratamento com radioterapia definitiva Métodos: Foram avaliados pacientes com câncer de pulmão não pequenas células, com estádios I–IV, que receberam radioterapia exclusiva ou combinada com quimioterapia, dirigida à neoplasia brônquica, na unidade de radioterapia do HCPA, nos anos de 2009 a 2015. Todos os pacientes tiveram a expressão de cofilina-1 aferida e foram distribuídos conforme os níveis de expressão de cofilina-1 utilizando-se de protocolo específico. Os prontuários foram avaliados retrospectivamente para calcular a sobrevida mediana. A progressão foi verificada através de avaliação de tomografias de tórax de controle. Resultados: Foram avaliados 45 pacientes neste estudo. A sobrevida mediana de todos os pacientes foi de 11,3 meses e a sobrevida global em 5 anos de 17,3%. Pacientes com expressão média ou alta de cofilina-1 tiveram maior mortalidade quando comparados com pacientes com baixa expressão (respectivamente, HR 1,628, IC95 1,137-8,287 e HR 1,59 IC95 1,105-7,342). Não houve diferença significantemente estatística entre controle local e expressão de cofilina-1. Conclusão: A expressão de cofilina-1 está associada à sobrevida em pacientes com carcinoma brônquico tratados com radioterapia e existe uma tendência a melhor controle local com baixa expressão. Nossos resultados sugerem um novo campo a ser explorado no manejo do carcinoma de pulmão localmente avançado, utilizando-se dos níveis de cofilina-1.Background: Lung cancer is a disease with high incidence and mortality, whose prognosis remains poor despite a better understanding of the disease in the last decades. Radiotherapy plays a therapeutic role in all stages of disease. The expression of cofilin-1, a protein related to cellular mobility, determined greater radiosensitivity to lung adenocarcinoma cells in in vitro studies, but worse survival at initial stages. Objective: To evaluate if the expression of cofilin-1 modified survival and local control in lung cancer patients submitted to definitive treatment with radiotherapy. Methods: Patients with non-small cell lung cancer with stage IIV who received radiotherapy alone or combined with chemotherapy for lung cancer at the HCPA radiotherapy unit from 2009 to 2015 were evaluated. All patients had the expression of measured cofilin-1 evaluated and were distributed by cofilin-1 expression according to specific protocol. The medical records were retrospectively evaluated to estimate median survival. The progression was verified through evaluation of control chest tomography. Results: 45 patients were assessed in this study. The median survival of all patients was 11.3 months and the 5-year overall survival was 17.3%. Patients with medium or high expression of cofilin-1 had higher mortality rates when compared to patients with low expression (HR, 1,628, CI95, 1,137-8,287 and HR, 1.59, CI95, 1,105-7,342). There was no statistically significant difference between local control and cofilin-1 expression. Conclusion: cofilin-1 expression is associated with survival in patients with lung cancer treated with radiotherapy and there is a tendency for better local control with low CFL1 expression. Our results suggest a new field to be explored in the management of locally advanced lung carcinoma, using cofilin-1 expression levels.
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Vice, President Research Office of the. "Mixed Messages." Office of the Vice President Research, The University of British Columbia, 2006. http://hdl.handle.net/2429/2676.
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Vice, President Research Office of the. "Wood Wide Web." Office of the Vice President Research, The University of British Columbia, 2006. http://hdl.handle.net/2429/2701.
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