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Journal articles on the topic 'Characterisation of molecular clusters'

Author: Grafiati

Published: 4 June 2021

Last updated: 6 February 2022

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1

Gomez, Jose L., Xiting Yan, Carole T. Holm, et al. "Characterisation of asthma subgroups associated with circulating YKL-40 levels." European Respiratory Journal 50, no. 4 (2017): 1700800. http://dx.doi.org/10.1183/13993003.00800-2017.

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The chitinase-like protein YKL-40 mediates airway inflammation and serum levels are associated with asthma severity. However, asthma phenotypes associated with YKL-40 levels have not been precisely defined.We conducted an unsupervised cluster analysis of asthma patients treated at the Yale Center for Asthma and Airways Disease (n=156) to identify subgroups according to YKL-40 level. The resulting YKL-40 clusters were cross-validated in cohorts from the Severe Asthma Research Programme (n=167) and the New York University/Bellevue Asthma Repository (n=341). A sputum transcriptome analysis reveal

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2

Al-Faifi, Sulieman A., Hussein M. Migdadi, Abedallah Al-doss, et al. "Morphological and molecular genetic variability analyses of Saudi lucerne (Medicago sativa L.) landraces." Crop and Pasture Science 64, no. 2 (2013): 137. http://dx.doi.org/10.1071/cp12271.

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Collection and characterisation of genetic resources are required for the development of new cultivars. We analysed genetic diversity among 18 non-dormant lucerne (alfalfa, Medicago sativa L.) accessions including ten local ecotypes and eight introduced accessions at morphological and molecular levels using sequence-related amplified polymorphism (SRAP) primers. Standardised canonical discrimination functions for the investigated morphological traits showed that the first function (explaining 75% of total variability among accessions) was strongly influenced by leaflet shape, stipule shape, an

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Pandey, S., and A. Alam. "Molecular Markers (RAPD and SSR) Based Characterisation of Genetic Diversity and Population Structure of Moss Hyophila Involuta." Acta Botanica Hungarica 63, no. 1-2 (2021): 171–93. http://dx.doi.org/10.1556/034.63.2021.1-2.10.

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Genetic relationships among 24 genotypes of Hyophila involuta collected from five different natural populations of Mount Abu (Rajasthan) is analysed using RAPD and SSR markers. Based on efficiency parameters calculated for each marker system such as polymorphic information content (RAPD = 0.34; SSR = 0.66), marker index (RAPD = 2.78; SSR = 2.62) and resolving power (RAPD = 8.13; SSR = 2.23), the RAPD marker system shows higher values for some indices but microsatellites are more accurately reproducible than RAPD. Moreover, in case of the SSR, the average number of alleles was almost twice comp

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4

Hui, Janesse Wing-Sze, and Wing-Tak Wong. "Synthesis, characterisation, molecular and crystal structures of two novel osmium–palladium carbide carbonyl clusters." J. Chem. Soc., Dalton Trans., no. 13 (1996): 2887–88. http://dx.doi.org/10.1039/dt9960002887.

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Dale, Michael J., Paul J. Dyson, Brian F. G. Johnson, Caroline M. Martin, Patrick R. R. Langridge-Smith, and Renato Zenobi. "Characterisation of ruthenium clusters carrying facial arene ligands using ultraviolet laser desorption mass spectrometry: aggregation of gas-phase monocharged anionic clusters from neutral molecular clusters." Journal of the Chemical Society, Chemical Communications, no. 16 (1995): 1689. http://dx.doi.org/10.1039/c39950001689.

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6

Jukanti, Aravind K., Phil L. Bruckner, and Andreas M. Fischer. "Molecular and biochemical characterisation of polyphenol oxidases in developing kernels and senescing leaves of wheat (Triticum aestivum)." Functional Plant Biology 33, no. 7 (2006): 685. http://dx.doi.org/10.1071/fp06050.

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Polyphenol oxidases (PPOs) have been implicated in plant defence reactions. From an applied point of view, high PPO activity is associated with browning / darkening of fresh and processed food. Owing to its complex genome and economic importance, wheat (Triticum aestivum L.) represents an interesting system to advance our understanding of plant PPO function. We have previously shown that wheat PPOs are organised in a multigene family, consisting of two distinct phylogenetic clusters with three members each. In this study, we demonstrate that members of one cluster are not expressed in developi

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TARGOŃSKA-KARASEK, MAŁGORZATA, HANNA BOLIBOK-BRĄGOSZEWSKA, TYMOTEUSZ OLENIECKI, SAIDA SHARIFOVA, MARTA KOPANIA, and MONIKA RAKOCZY-TROJANOWSKA. "Verification of taxonomic relationships within the genus Secale (Poaceae: Pooideae: Triticeae) based on multiple molecular methods." Phytotaxa 383, no. 2 (2018): 128. http://dx.doi.org/10.11646/phytotaxa.383.2.1.

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This study aimed to verify the taxonomic relationships within the genus Secale. The plant material included 16 wild rye accessions from four species. Two approaches were applied: 1) whole genome scanning using three molecular marker systems: diversity arrays technology sequencing, simple sequence repeats and sequence-specific amplification polymorphism; and 2) characterisation based on polymorphisms within the sequences of two genes involved in benzoxazinoid biosynthesis: ScBx1 and ScBx5. Bayesian and neighbour-joining clustering and principal coordinate analysis were applied to illustrate rel

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Tveten, Ann-Kristin. "Exploring Diversity among NorwegianBorreliaStrains Originating fromIxodes ricinusTicks." International Journal of Microbiology 2014 (2014): 1–10. http://dx.doi.org/10.1155/2014/397143.

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Characterisation ofBorreliastrains fromIxodes ricinusticks is important in the epidemiological surveillance of vector-borne pathogens. Multilocus sequences analysis (MLSA) is a molecular genotyping tool with high discriminatory power that has been applied in evolutionary studies and for the characterisation ofBorreliagenospecies. MLSA was used to study genetic variations inBorreliastrains isolated fromI. ricinusticks collected from the woodlands in Skodje. The results demonstrate that the 50Borreliastrains were separated into 36 sequence types (STs) that were not previously represented in the

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Saddi-Ortega, L., M. A. R. Carvalho, P. S. Cisalpino, and E. S. A. Moreira. "Actinobacillus actinomycetemcomitansgenetic heterogeneity: amplification of JP2-likeltxpromoter pattern correlated with specific arbitrarily primed polymerase chain reaction (AP-PCR) genotypes from human but not marmoset Brazilian isolates." Canadian Journal of Microbiology 48, no. 7 (2002): 602–10. http://dx.doi.org/10.1139/w02-055.

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Specific clonal types of Actinobacillus actinomycetemcomitans, a major human periodontal pathogen, may be responsible for clinical manifestations and the production of leukotoxin virulence factors. Leukotoxicity is associated with genetic polymorphism at the promoter region of the leukotoxin (ltx) gene. Here, we describe the use of arbitrarily primed polymerase chain reaction (AP-PCR) and ltx promoter PCR to molecularly characterise 35 A. actinomycetemcomitans Brazilian isolates: 21 of human origin and 14 from captive marmosets (Callitrix spp., primates commonly used as animal models for perio

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Kumar, Jitendra, Partha Sarathi Basu, Sunanda Gupta, Sonali Dubey, Debjyoti Sen Gupta, and Narendra Pratap Singh. "Physiological and molecular characterisation for high temperature stress in Lens culinaris." Functional Plant Biology 45, no. 4 (2018): 474. http://dx.doi.org/10.1071/fp17211.

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In the present study, 11 lentil (Lens culinaris Medik) genotypes including heat tolerant and heat sensitive genotypes identified after a screening of 334 accessions of lentil for traits imparting heat tolerance, were characterised based on physiological traits and molecular markers. Results showed a higher reduction in pollen viability among sensitive genotypes (up to 52.3%) compared with tolerant genotypes (up to 32.4%) at 43°C. Higher photosynthetic electron transport rate was observed among heat tolerant genotypes and two heat tolerant lentil genotypes, IG 4258 (0.43) and IG 3330 (0.38) wer

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Fazal, Asif, Divya Thankachan, Ellie Harris, and Ryan F. Seipke. "A chromatogram-simplified Streptomyces albus host for heterologous production of natural products." Antonie van Leeuwenhoek 113, no. 4 (2019): 511–20. http://dx.doi.org/10.1007/s10482-019-01360-x.

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AbstractCloning natural product biosynthetic gene clusters from cultured or uncultured sources and their subsequent expression by genetically tractable heterologous hosts is an essential strategy for the elucidation and characterisation of novel microbial natural products. The availability of suitable expression hosts is a critical aspect of this workflow. In this work, we mutagenised five endogenous biosynthetic gene clusters from Streptomyces albus S4, which reduced the complexity of chemical extracts generated from the strain and eliminated antifungal and antibacterial bioactivity. We showe

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Quinn, Peter J. "Characterisation of Clusters of alpha-Tocopherol in Gel and Fluid Phases of Dipalmitoylglycerophosphocholine." European Journal of Biochemistry 233, no. 3 (1995): 916–25. http://dx.doi.org/10.1111/j.1432-1033.1995.916_3.x.

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Krajewska-Wędzina, Monika, Monika Kozińska, Łukasz Radulski, et al. "Molecular characterisation of the Mycobacterium bovis causing bovine tuberculosis outbreaks in Poland." Journal of Veterinary Research 64, no. 1 (2020): 45–50. http://dx.doi.org/10.2478/jvetres-2020-0003.

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AbstractIntroductionSince 2009, Poland has been recognised as a country officially free of bovine tuberculosis (bTB), although in each year of the last five there were from 8 to 18 outbreaks of the disease. In 2008–2016, the largest number of cattle infected with bovine mycobacteria were eliminated in the Masovian Province (the central region of Poland) and the largest number of outbreaks of this zoonosis were recorded in this area. The close proximity of farms where bTB was found led to the suspicion that tuberculosis could have been transmitted between the affected herds. The aim of the stud

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Akhmanova, A., T. Verkerk, A. Langeveld, F. Grosveld, and N. Galjart. "Characterisation of transcriptionally active and inactive chromatin domains in neurons." Journal of Cell Science 113, no. 24 (2000): 4463–74. http://dx.doi.org/10.1242/jcs.113.24.4463.

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The tandemly organised ribosomal DNA (rDNA) repeats are transcribed by a dedicated RNA polymerase in a specialised nuclear compartment, the nucleolus. There appears to be an intimate link between the maintenance of nucleolar structure and the presence of heterochromatic chromatin domains. This is particularly evident in many large neurons, where a single nucleolus is present, which is separated from the remainder of the nucleus by a characteristic shell of heterochromatin. Using a combined fluorescence in situ hybridisation and immunocytochemistry approach, we have analysed the molecular compo

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Weber, Tim, Jernej Jakše, Barbara Sladonja, et al. "Molecular Study of Selected Taxonomically Critical Taxa of the Genus Iris L. from the Broader Alpine-Dinaric Area." Plants 9, no. 9 (2020): 1229. http://dx.doi.org/10.3390/plants9091229.

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Some wild, morphologically diverse taxa of the genus Iris in the broad Alpine-Dinaric area have never been explored molecularly, and/or have ambiguous systematic status. The main aims of our research were to perform a molecular study of critical Iris taxa from that area (especially a narrow endemic accepted species I. adriatica, for which we also analysed genome size) and to explore the contribution of eight microsatellites and highly variable chloroplast DNA (ndhJ, rpoC1) markers to the understanding of the Iris taxa taxonomy and phylogeny. Both the microsatellite-based UPGMA and plastid mark

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Adjei, Cosmos B., Usha Govinden, Krishnee Moodley, and Sabiha Y. Essack. "Molecular characterisation of multidrug-resistant Pseudomonas aeruginosa from a private hospital in Durban, South Africa." Southern African Journal of Infectious Diseases 33, no. 2 (2018): 38–41. http://dx.doi.org/10.4102/sajid.v33i2.19.

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Background: Multi-drug resistant Pseudomonas aeruginosa pose a clinical challenge globally. This study delineated the molecular mechanisms of resistance to β-lactam antibiotics in multidrug-resistant P. aeruginosa isolated from a single private hospital in Durban, South Africa and ascertained clonality with regard to the isolates carrying β-lactamase genes.Methods: Seventeen P. aeruginosa isolates recovered from sputum, urine, catheter tips, pus swabs, nasal swabs and endotracheal aspirates underwent MIC determination, and phenotypic screening using the Double Disk Synergy Test (DDST) and Modi

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17

Okukenu, O. A., A. A. Olajide, P. A. Dele, et al. "Microsatellite markers-based characterisation of elephant grass (Pennisetum purpureum) harvested from selected locations in South-West Nigeria." Nigerian Journal of Biotechnology 37, no. 1 (2020): 38–45. http://dx.doi.org/10.4314/njb.v37i1.4.

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This study was carried out to characterise Pennisetum purpureum harvested from some selected locations in S outh-W estern Nigeria using microsatellite markers. Leaf parts of growing young elephant grass (Pennisetum purpureum) were harvested and immediately preserved in ethanol solution before DNA extraction. Two (2) SSR primers (CTM59 and Xtxp278) were used to assess genetic diversity in Pennisetum purpureum. The result shows that 72% of the molecular variations in the elephant grass exists within the population with 28% among the population; there were no unique characteristics among the Nine

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Amoroso, Angelo J., Brian F. G. Johnson, Jack Lewis, Paul R. Raithby, and Wing Tak Wong. "Synthesis and characterisation of nona-osmium carbonyl clusters; crystal and molecular structure of [(Ph3P)2N][Os9H(CO)24]." Journal of the Chemical Society, Chemical Communications, no. 12 (1991): 814. http://dx.doi.org/10.1039/c39910000814.

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de Glanville, William A., Alicia Davis, Kathryn J. Allan, et al. "Classification and characterisation of livestock production systems in northern Tanzania." PLOS ONE 15, no. 12 (2020): e0229478. http://dx.doi.org/10.1371/journal.pone.0229478.

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Livestock keepers in sub-Saharan Africa face a range of pressures, including climate change, land loss, restrictive policies, and population increase. Widespread adaptation in response can lead to the emergence of new, non-traditional typologies of livestock production. We sought to characterise livestock production systems in two administrative regions in northern Tanzania, an area undergoing rapid social, economic, and environmental change. Questionnaire and spatial data were collected from 404 livestock-keeping households in 21 villages in Arusha and Manyara Regions in 2016. Multiple factor

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20

Fabeni, Lavinia, Claudia Alteri, Giulia Berno, et al. "Characterisation of HIV-1 molecular transmission clusters among newly diagnosed individuals infected with non-B subtypes in Italy." Sexually Transmitted Infections 95, no. 8 (2019): 619–25. http://dx.doi.org/10.1136/sextrans-2019-054017.

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ObjectiveWe evaluated the characteristics of HIV-1 molecular transmission clusters (MTCs) in 1890 newly diagnosed individuals infected with non-B subtypes between 2005 and 2017 in Italy.MethodsPhylogenetic analyses were performed on pol sequences to characterise subtypes/circulating recombinant forms and identify MTCs. MTCs were divided into small (SMTCs, 2–3 sequences), medium (MMTCs, 4–9 sequences) and large (LMTCs, ≥10 sequences). Factors associated with MTCs were evaluated using logistic regression analysis.Results145 MTCs were identified and involved 666 individuals (35.2%); 319 of them (

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Goredema, N., T. Ndowora, R. Shoko, and E. Ngadze. "Morphological and molecular characterisation of Streptomyces spp. which suppress pathogenic fungi." African Crop Science Journal 28, no. 4 (2020): 555–66. http://dx.doi.org/10.4314/acsj.v28i4.6.

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Streptomyces species are aerobes and chemoorganotrophic bacteria. These microorganisms produce a wide range of industrially significant compounds, specifically antibiotics and anti fungal substances. The objective of this study was to characterise soil-borne Streptomyces isolates using morphological and molecular traits in order to identify them to species level, and leverage from their potential to suppress the growth of Aspergillus flavus, Fusarium oxysporum and Penicillium italicum. Twenty-seven soil-borne putative Streptomyces, which elicited comprehensive antimicrobial activity against As

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Hu, Wen, Xin Xu, Qiang Liu, et al. "Molecular characterisation and genetic diversity of canine parvovirus type 2 prevalent in Central China." Journal of Veterinary Research 64, no. 3 (2020): 347–54. http://dx.doi.org/10.2478/jvetres-2020-0056.

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AbstractIntroductionCanine parvovirus (CPV) disease is one of the most threatening to domestic and wild dogs.Material and MethodsA total of 132 clinical samples were isolated from domestic dogs with diarrhoea from Henan, Hubei, Jiangsu, and Anhui provinces from 2016 to 2017, and 56 were positive for CPV-2 by PCR. A phylogenetic tree was constructed for the isolate sequences incorporating 53 non-Chinese reference strains.ResultsVP2 sequences showed the strains mainly to be new CPV-2a/2b and CPV-2c genotypes. The Ala5Gly, Phe267Tyr, Ser297Ala, Tyr324Ile, Gln370Arg, Asn426Asp or Asn426Glu, and Th

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Braga, Dario, Fabrizia Grepioni, Paul J. Dyson, Brian F. G. Johnson та Caroline M. Martin. "Preparation, characterisation, molecular and crystal structure of the octaruthenium arene clusters [Ru8H4(CO)18(η6-arene)](arene = C6H6or C16H16)". J. Chem. Soc., Dalton Trans., № 6 (1995): 909–16. http://dx.doi.org/10.1039/dt9950000909.

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Karamzadeh, Baharan, Thomas Eaton, David Muñoz Torres, Izabela Cebula, Marcel Mayor, and Manfred Buck. "Sequential nested assembly at the liquid/solid interface." Faraday Discussions 204 (2017): 173–90. http://dx.doi.org/10.1039/c7fd00115k.

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Studying the stepwise assembly of a four component hybrid structure on Au(111)/mica, the pores of a hydrogen bonded bimolecular network of 3,4,9,10-perylenetetracarboxylic diimide (PTCDI) and 1,3,5-triazine-2,4,6-triamine (melamine) were partitioned by three and four-armed molecules based on oligo([biphenyl]-4-ylethynyl)benzene, followed by the templated adsorption of either C<sub>60</sub> fullerene or adamantane thiol molecules. The characterisation by ambient scanning tunneling microscopy (STM) reveals that the pore modifiers exhibit dynamics which pronouncedly depend on the molecular struct

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Špunarová, M., J. Ovesná, L. Tvarůžek, L. Kučera, J. Špunar, and I. Hollerová. "The use of molecular markers for characterisation of spring barley for breeding to Fusarium head blight resistance." Plant, Soil and Environment 51, No. 11 (2011): 483–90. http://dx.doi.org/10.17221/3621-pse.

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Fusarium head blight (FHB) is a barley disease, which occurs every year in various areas of barley cultivation all over the world and the increasing incidence has been confirmed in the Czech Republic also during the last years. We aimed to emply AFLP (Amplified fragment length polymorphism) and SSR (Single sequence repeats) markers to describe diversity among breeding lines with a sufficient level of resistance towards FHB and to find marker(s) associated with the analysed traits. The number of eight accessions including five expected resistance donors and three sensitive lines were tested in

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Drees, Alissa, and Markus Fischer. "High-Throughput Selection and Characterisation of Aptamers on Optical Next-Generation Sequencers." International Journal of Molecular Sciences 22, no. 17 (2021): 9202. http://dx.doi.org/10.3390/ijms22179202.

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Aptamers feature a number of advantages, compared to antibodies. However, their application has been limited so far, mainly because of the complex selection process. ‘High-throughput sequencing fluorescent ligand interaction profiling’ (HiTS–FLIP) significantly increases the selection efficiency and is consequently a very powerful and versatile technology for the selection of high-performance aptamers. It is the first experiment to allow the direct and quantitative measurement of the affinity and specificity of millions of aptamers simultaneously by harnessing the potential of optical next-gen

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Shin, Sang, Ji Kim, Casiano Choresca, Jee Han, Jin Jun, and Se Park. "Molecular identification and phylogenetic characterisation of Thelohanellus kitauei — short communication." Acta Veterinaria Hungarica 61, no. 1 (2013): 30–35. http://dx.doi.org/10.1556/avet.2012.055.

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Thelohanellus kitauei was isolated from the koi Cyprinus carpio haematopterus, and the 18S rRNA gene of T. kitauei was amplified by optimised nested-PCR. The PCR product was sequenced and compared with other 18S rRNA genes of Thelohanellus species to investigate the relationships between their host specificities and infection sites. Based on the 18S rRNA sequences, T. kitauei is most closely related to T. hovorkai (which can infect the intestine). Phylogenetic analysis revealed that T. kitauei was clustered with other Thelohanellus spp. infecting Cyprininae. The present study suggests that the

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Wang, Haifeng, Liyijing Shen, Yaoqing Li, and Jieqing Lv. "Integrated characterisation of cancer genes identifies key molecular biomarkers in stomach adenocarcinoma." Journal of Clinical Pathology 73, no. 9 (2020): 579–86. http://dx.doi.org/10.1136/jclinpath-2019-206400.

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AimsGastric cancer is one of the leading causes for cancer mortality. Recent studies have defined the landscape of genomic alterations of gastric cancer and their association with clinical outcomes. However, the pathogenesis of gastric cancer has not been completely characterised.MethodsDriver genes were detected by five computational tools, MutSigCV, OncodriveCLUST, OncodriveFM, dendrix and edriver, using mutation data of stomach adenocarcinoma (STAD) from the cancer genome altas database, followed by an integrative investigation.ResultsTTN, TP53, LRP1B, CSMD3, OBSCN, ARID1A, FAT4, FLG, PCLO

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Hosseinvand, Manouchehr, Ali Eskandari, and Reza Ghaderi. "Morphological and molecular characterisation of three known species of Criconematoidea from Iran." Nematology 22, no. 7 (2020): 745–58. http://dx.doi.org/10.1163/15685411-00003337.

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Summary In this study, we provide morphological and molecular characterisation of three known species of the Criconematoidea, namely Criconema cylindraceum, Paratylenchus alleni and P. steineri, recovered from Iran. Descriptions, morphometric data, drawings and photomicrographs are provided, and comparisons are made with closely related species. Phylogenetic analysis using the D2-D3 expansion segments of the 28S rRNA gene revealed that C. cylindraceum formed a cluster with Ogma decalineatus, a species with a similar head structure, but with differences in cuticle ornamentation, tail shape and

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Trzmiel, Katarzyna, Wiktoria Szydło, and Beata Hasiów-Jaroszewska. "Biological and molecular characterisation of the two Polish Wheat streak mosaic virus isolates and their transmission by wheat curl mites." Plant Protection Science 57, No. 3 (2021): 171–78. http://dx.doi.org/10.17221/104/2020-pps.

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Wheat streak mosaic virus (WSMV) is a serious and widespread pathogen in the wheat-producing areas in the USA while, in Europe, it has been considered a minor threat to cereal crops. In the past, WSMV was detected in wheat, triticale and maize plants in Poland by DAS-ELISA. Here, we present the biological and molecular characterisation of WSMV-Sze and WSMV-Sosn isolates collected from western and southern Poland and report their transmissibility by the widespread wheat curl mite (WCM) lineage MT-8. The performed bioassays revealed that the analysed WSMV isolates infect wheat, barley, triticale

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Nianyong, Zhu, Wu Xintao, and Lu Jiaxi. "The synthesis and characterisation of two novel heterometallic trinuclear incomplete cubane-like clusters [Et4N](M2AgS4)(S2C2H4)2(PPh3)·CH2Cl2(M = Mo, W)." J. Chem. Soc., Chem. Commun., no. 4 (1991): 235–37. http://dx.doi.org/10.1039/c39910000235.

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Jarman, A. P., R. D. Nicholls, D. J. Weatherall, J. B. Clegg, and D. R. Higgs. "Molecular characterisation of a hypervariable region downstream of the human alpha-globin gene cluster." EMBO Journal 5, no. 8 (1986): 1857–63. http://dx.doi.org/10.1002/j.1460-2075.1986.tb04437.x.

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Bould, Jonathan, Simon J. Teat та John D. Kennedy. "Polyhedral Dipalladaborane Chemistry. The Molecular Structure and Cluster Electron Count of [7,8-(PPh3)2-7,8-(μ-PPh2)-9,11-(OEt)2-nido-7,8-Pd2B9H8]". Collection of Czechoslovak Chemical Communications 72, № 12 (2007): 1631–38. http://dx.doi.org/10.1135/cccc20071631.

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[PdCl2(PPh3)2] and the [NHEt3]+ salt of [B10H10]2- in refluxing ethanol yield the dipalladaundecaborane cluster compound [7,8-(PPh3)2-7,8-(μ-PPh2)-9,11-(OC2H5)2-nido-7,8-Pd2B9H8] (1). An all-atom single-crystal synchrotron X-ray diffraction study shows a significant difference to four structurally characterised platinum congeners reported in the literature for which revised formulation is now necessary. The importance of taking into consideration the electron count in polyhedral boron-containing cluster systems in the course of their characterisation is emphasised.

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Brauer, Marieke, Marianne Wolfaardt, Lynne M. Webber, and Maureen B. Taylor. "Molecular detection and characterisation of mumps virus in cerebrospinal fluid in a Gauteng laboratory." Southern African Journal of Infectious Diseases 31, no. 1 (2016): 29–31. http://dx.doi.org/10.4102/sajid.v31i1.102.

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The study aimed to determine the presence of mumps virus (MuV) in cerebrospinal fluid (CSF) specimens and to genetically characterise detected MuV strains. A real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the MuV F gene, and characterisation was performed by sequencing of the SH gene. Mumps virus was detected in 1.2% (3/260) of specimens. Phylogenetic analysis of one MuV strain revealed that it clustered with the Jeryl-Lynn and RIT4385 vaccine strains. As far as the authors could ascertain this is the first study to provide viral proof that these vaccine-

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RAMOS, C., F. FRANCO, D. SMITH, and S. ULIANA. "Characterisation of a new gene and genomic analysis of the cluster." FEMS Microbiology Letters 238, no. 1 (2004): 213–19. http://dx.doi.org/10.1016/j.femsle.2004.07.037.

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Alberti, Fabrizio, Saraa Kaleem, and Jack A. Weaver. "Recent developments of tools for genome and metabolome studies in basidiomycete fungi and their application to natural product research." Biology Open 9, no. 12 (2020): bio056010. http://dx.doi.org/10.1242/bio.056010.

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ABSTRACTBasidiomycota are a large and diverse phylum of fungi. They can make bioactive metabolites that are used or have inspired the synthesis of antibiotics and agrochemicals. Terpenoids are the most abundant class of natural products encountered in this taxon. Other natural product classes have been described, including polyketides, peptides, and indole alkaloids. The discovery and study of natural products made by basidiomycete fungi has so far been hampered by several factors, which include their slow growth and complex genome architecture. Recent developments of tools for genome and meta

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Amoroso, Angelo J., Brian F. G. Johnson, Jack Lewis, Paul R. Raithby, and Wing-Tak Wong. "Synthesis and characterisation of heptaosmium carbido clusters; crystal and molecular structures of [Os7(µ-H)2C(CO)19] and [Os7(µ-H)C(CO)19]–." J. Chem. Soc., Chem. Commun., no. 17 (1990): 1208–9. http://dx.doi.org/10.1039/c39900001208.

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Tagliani, Elisa, Richard Anthony, Thomas A. Kohl, et al. "Use of a whole genome sequencing-based approach for Mycobacterium tuberculosis surveillance in Europe in 2017–2019: an ECDC pilot study." European Respiratory Journal 57, no. 1 (2020): 2002272. http://dx.doi.org/10.1183/13993003.02272-2020.

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Whole genome sequencing (WGS) can be used for molecular typing and characterisation of Mycobacterium tuberculosis complex (MTBC) strains. We evaluated the systematic use of a WGS-based approach for MTBC surveillance involving all European Union/European Economic Area (EU/EEA) countries and highlight the challenges and lessons learnt to be considered for the future development of a WGS-based surveillance system.WGS and epidemiological data of patients with rifampicin-resistant (RR) and multidrug-resistant (MDR) tuberculosis (TB) were collected from EU/EEA countries between January 2017 and Dece

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Nieto-Taladriz, M. T., M. Rodríguez-Quijano, and J. M. Carrillo. "Biochemical and genetic characterisation of a D glutenin subunit encoded at the Glu-B3 locus." Genome 41, no. 2 (1998): 215–20. http://dx.doi.org/10.1139/g98-010.

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The SDS-PAGE pattern of reduced and alkylated glutenins from the bread wheat cultivar Prinqual presents a subunit (named d4) in the mobility zone of the omega -gliadins that only appears under reduced conditions. This subunit was isolated and characterised at the biochemical and genetic levels. Subunit d4 was shown to form disulphide aggregates with glutenins and had an acidic pI. These characteristics correspond to those of the D glutenin subunits. The N-terminal amino acid sequence of subunit d4 was coincident with the SRL sequence type characteristic of omega -gliadins encoded by genes on t

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Renčo, Marek, Václav Čermák, Kateřina Tománková, and Mária Majeská Čudejková. "Morphological and molecular characterisation of Heterodera filipjevi (Madzhidov, 1981) from the Slovak Republic." Nematology 20, no. 3 (2018): 253–64. http://dx.doi.org/10.1163/15685411-00003138.

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Cysts, males and juveniles from a population of Heterodera filipjevi found on sports-ground turf in Trnava, Slovak Republic, in 2016 are described based on morphology and morphometrics. The identity of species of juveniles extracted from cysts was subsequently determined and confirmed by PCR-RFLP and sequencing. Morphologically, cysts, juveniles and males from the Slovak Republic are similar to paratypes from Tajikistan. The results of the phylogenetic analysis of the ITS rRNA gene sequences confirmed the species identification and phylogenetic relationship of H. filipjevi with other Heteroder

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Gallo, Antonia, and Giancarlo Perrone. "Current Approaches for Advancement in Understanding the Molecular Mechanisms of Mycotoxin Biosynthesis." International Journal of Molecular Sciences 22, no. 15 (2021): 7878. http://dx.doi.org/10.3390/ijms22157878.

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Filamentous fungi are able to synthesise a remarkable range of secondary metabolites, which play various key roles in the interaction between fungi and the rest of the biosphere, determining their ecological fitness. Many of them can have a beneficial activity to be exploited, as well as negative impact on human and animal health, as in the case of mycotoxins contaminating large quantities of food, feed, and agricultural products worldwide and posing serious health and economic risks. The elucidation of the molecular aspects of mycotoxin biosynthesis has been greatly sped up over the past deca

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Timurkan, Mehmet Ozkan, Hakan Aydin, and Ahmet Sait. "Identification and molecular characterisation of bovine parainfluenza virus-3 and bovine respiratory syncytial virus - first report from Turkey." Journal of Veterinary Research 63, no. 2 (2019): 167–73. http://dx.doi.org/10.2478/jvetres-2019-0022.

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AbstractIntroduction:Bovine parainfluenza virus-3 (BPIV3) and bovine respiratory syncytial virus (BRSV) are the cause of respiratory disease in cattle worldwide. With other pathogens, they cause bovine respiratory disease complex (BRDC) in ruminants. The aim of the study was the detection and molecular characterisation of BPIV3 and BRSV from nasal swabs and lung samples of cows in and around the Erzurum region of eastern Turkey.Material and Methods:In total, 155 samples were collected. Of animals used in the study 92 were males and 63 females. The age of the animals was between 9 months and 5

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Palomares-Rius, Juan E., Carolina Cantalapiedra-Navarrete, Carlos Gutiérrez-Gutiérrez, Gracia Liébanas, and Pablo Castillo. "Morphological and molecular characterisation of Paralongidorus plesioepimikis n. sp. (Nematoda: Longidoridae) from southern Spain." Nematology 15, no. 3 (2013): 363–78. http://dx.doi.org/10.1163/15685411-00002680.

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Paralongidorus plesioepimikis n. sp. is described and illustrated by light microscopy, scanning electron microscopy and molecular studies from specimens collected in a sandy soil in the rhizosphere of stone pine (Pinus pinea) in Bonares (Huelva Province), southern Spain. The new species is characterised by a very long body (9.71-14.11 mm), an expanded and rounded lip region, with a clear constriction followed by a depression posterior to the amphidial aperture, a very long and flexible odontostyle (202-227 μm), guiding ring located at 36.5-44.0 μm from anterior end, dorsal pharyngeal gland nuc

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Wallis, OC, and M. Wallis. "Characterisation of the GH gene cluster in a new-world monkey, the marmoset (Callithrix jacchus)." Journal of Molecular Endocrinology 29, no. 1 (2002): 89–97. http://dx.doi.org/10.1677/jme.0.0290089.

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In most mammals pituitary GH is encoded by a single gene with no close relatives. However, in man the GH gene has been shown to be one of a cluster of five closely related genes, four of which are expressed in the placenta. Rhesus monkey also expresses at least five closely related GH-like genes, although the genomic organisation of these has not been fully reported. Here we describe the cloning and characterisation of GH-like genes in a new-world monkey, the marmoset (Callithrix jacchus). This species possesses a cluster of eight GH-like 'genes'. The gene at the 5' end of this cluster encodes

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Chiesa, Anna, Renata Ugo, Angelo Sironi, and Anatoli Yatsimirski. "Isolation and characterisation by X-ray studies of palladium µ2-nitrosyl clusters in the reduction of nitric acid by carbon monoxide mediated by palladium acetate." J. Chem. Soc., Chem. Commun., no. 4 (1990): 350–51. http://dx.doi.org/10.1039/c39900000350.

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Blake, Alexander J., Paul J. Dyson, Rosslyn C. Gash, Brian F. G. Johnson та Peter Trickey. "Bis(cyclopentadienyl) clusters of ruthenium: the synthesis, characterisation and molecular structures of [Ru5C(CO)10(η5-C5H5)2] and [Ru6C(CO)12(η5-C5H5)2]". J. Chem. Soc., Dalton Trans., № 7 (1994): 1105–8. http://dx.doi.org/10.1039/dt9940001105.

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Shephard, Douglas S., Thomas Maschmeyer, Gopinathan Sankar, et al. "Preparation, Characterisation and Performance of Encapsulated Copper-Ruthenium Bimetallic Catalysts Derived from Molecular Cluster Carbonyl Precursors." Chemistry - A European Journal 4, no. 7 (1998): 1214–24. http://dx.doi.org/10.1002/(sici)1521-3765(19980710)4:7<1214::aid-chem1214>3.0.co;2-e.

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Vaithilingam, Vijayaganapathy, Cherry Fung, Sabina Ratnapala, et al. "Characterisation of the Xenogeneic Immune Response to Microencapsulated Fetal Pig Islet-Like Cell Clusters Transplanted into Immunocompetent C57BL/6 Mice." PLoS ONE 8, no. 3 (2013): e59120. http://dx.doi.org/10.1371/journal.pone.0059120.

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Johnson, Brian F. G., Caroline M. Martin, Dario Braga, Fabrizia Grepioni та Emilio Parisini. "The synthesis and characterisation of the octaruthenium–benzene cluster [Ru8H4(CO)18(η6-C6H6)]". J. Chem. Soc., Chem. Commun., № 10 (1994): 1253–54. http://dx.doi.org/10.1039/c39940001253.

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Evans, John, Andrew C. Street, and Michael Webster. "Synthesis and structural characterisation of the hexanuclear, bimetallic, ladder-like cluster HRu5Cu(CO)18PPh3." Journal of the Chemical Society, Chemical Communications, no. 9 (1987): 637. http://dx.doi.org/10.1039/c39870000637.

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