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Journal articles on the topic 'CHARACTERISTICS ONMANNITOL SALT AGAR'

Author: Grafiati

Published: 3 June 2025

Last updated: 1 August 2025

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1

Farah, Tufail Hafiz Maqbool Ahmed Waqas Inayat. "THE SENSE OF DISTRIBUTION OF STAPHYLOCOCCUS AUREUS, THEIR CHARACTERISTICSON NUTRIENT AGAR, CHARACTERISTICS ONMANNITOL SALT AGAR, GROWTH CHARACTERISTICS ON STEPH-110 MEDIUM AND GROWTH CHARACTERISTICS ON BLOOD AGAR AND THEIR HEMOLYTIC PROPERTIES." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES 05, no. 10 (2018): 9868–81. https://doi.org/10.5281/zenodo.1461035.

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<em>Microorganisms, which are largely found in our environment, play an important role in stabilizing ecosystems such as primary energy and basic cycle. Microorganisms are everywhere like sea salts, high air pressure, very high and low temperatures. They are abundant in highly contaminated areas due to resistance. These organisms can be classified by ordinary carbon and energy sources converted into amino acids, starches, nucleotides, vitamins and different oils. Catalysts are mechanically extracted from microbes to perform different metabolic processes that are present in the world, and their

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Abdilah, Fuad, and Kurniawan Kurniawan. "MORPHOLOGICAL CHARACTERISTICS OF AIR BACTERIA IN MANNITOL SALT AGAR MEDIUM." Borneo Journal of Medical Laboratory Technology 5, no. 1 (2022): 353–59. http://dx.doi.org/10.33084/bjmlt.v5i1.4438.

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Udara merupakan salah satu unsur penting bagi kehidupan sehingga kualitas dan komponen yang terkandung didalamnya harus dikontrol, mengingat udara merupakan salah satu media penyebaran bakteri patogen. Kualitas udara yang baik sangat dibutuhkan oleh pasien di rumah sakit, tetapi faktanya banyak ditemukan bakteri patogen penyebab infeksi nosokomial di ruang rawat inap. Penelitian ini bertujuan untuk mengetahui karakteristik morfologi bakteri udara pada ruang rawat inap yang mampu tumbuh pada medium selektif Mannitol Salt Agar (MSA). Penelitian ini dilakukan menggunakan metode observasional deng

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Huq, Anwarul, Anwari Akhtar, M. A. R. Chowdhury, and David A. Sack. "Optimal growth temperature for the isolation of Plesiomonas shigelloides, using various selective and differential agars." Canadian Journal of Microbiology 37, no. 10 (1991): 800–802. http://dx.doi.org/10.1139/m91-138.

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The growth characteristics of known strains of Plesiomonas shigelloides were compared with those of Aeromonas species (the major competing species in environmental waters) on plesiomonas differential agar, inositol brilliant green bile salt, and modified salmonella–shigella agar at incubation temperatures of 37, 42, and 44 °C. Using local isolates from clinical and environmental sources, optimal growth conditions, as determined by colony counts and the colony characteristics, plesiomonas differential agar proved to be ideal when incubated at 44 °C. Contrary to earlier recommendations for 48 h

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Echeonwu, U. T., and O. C. Eruteya. "Bacteriological assessment of kunu-zaki sold in selected communities in Port Harcourt, Nigeria." Journal of Agriculture and Food Sciences 21, no. 2 (2024): 96–106. http://dx.doi.org/10.4314/jafs.v21i2.7.

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Kunu-zaki is a fermented beverage sold in Nigeria; often prepared under unsafe conditions, predisposing it to pathogen contaminations. The bacteria associated with Kunu-zaki sold in the different area of Port Harcourt and an antibiogram of the pathogens was determined using standard methods involving nutrient agar, Salmonella-Shigella agar, Thiosulphate citrate bile salt sucrose agar, MacConkey agar, Mannitol salt agar, Eosin-Methylene blue agar and Mueller Hinton agar. The counts for kunu-zaki obtained from Choba, Mile 1, Rumuodara and Rumuokoro ranged from 2.87±0.11 to 5.39±0.19, 4.50±0.27 t

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Mahulette, Ferymon, Mery Pattipeilohy, and Alamanda Pelamonia. "KELIMPAHAN DAN KARAKTERISTIK BAKTERI DALAM BAKASANG BIA GARU (Tridacna gigas L) [Abundance and Characteristics of Bacteria in Bakasang Bia Garu (Tridacna gigas)]." Berita Biologi 22, no. 3 (2023): 283–90. http://dx.doi.org/10.55981/beritabiologi.2023.697.

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Bakasang bia garu is a clam-based fermented product processed by the people of the Babar Islands, Maluku. Uncontrolled processing allows the presence of unwanted bacteria in these local food products. Preservatives added in the processing of bakasang bia garu are very diverse. This study aims to isolate and characterize lactic acid, halotolerant, and coliform bacteria from bakasang bia garu which are processed using different preservatives. Lactic acid bacteria were isolated using de Man Rogosa and Sharpe Agar (MRSA) media while halotolerant and coliform bacteria used Mannitol Salt Agar (MSA)

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Garuba, Taofeeq, Nency Katrodiya, Nikita Patel, et al. "Antibacterial activity of Urena lobata against uropathogens." Nigerian Journal of Natural Products and Medicine 25, no. 1 (2021): 43–46. http://dx.doi.org/10.4314/njnpm.v25i1.3.

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Urinary tract infections (UTI) are one of the most common form of bacterial infections but the treatment becomes cumbersome as the etiological bacteria are developing resistance against antibiotics. This present study evaluated the efficacy of antimicrobial activity of Urena lobata against uropathogens. Six urine samples from UTI patients were collected from Pathological Laboratory, G.B. Vaghani Multispecialty Hospital, Surat. Bacteria were isolated from these samples using Nutrient agar, Mac Conkey agar plate, Blood agar, Mannitol salt agar, Eosin Methylene Blue agar and King’s agar. The bact

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SILAS D GYAR and BILKIS MUNIR NAGOGO. "Study of some physicochemical and bacteriological characteristics of drinking water in three selected rural communities in Wamba, Nasarawa State, Nigeria." International Journal of Science and Research Archive 13, no. 1 (2024): 2986–93. http://dx.doi.org/10.30574/ijsra.2024.13.1.2024.

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Access to safe drinking water remains a challenge in rural Nigeria. This study was carried out to determine physicochemical and bacteriological characteristics of drinking water in three rural communities in Wamba, Nasarawa State. A cross-sectional survey was conducted, 5 water samples were in respective cases collected from stream, well and borehole sources. Standard techniques were used for physicochemical analysis. Total viable counts, Total Staphylococcal counts, Total coliforms & Feacal coliforms in the samples were obtained using pour plate method, growth on Mannitol salt agar, growt

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Sultana, S., MA Islam, and MM Khatun. "Bacteria in chicken rolls sold by fast food restaurant and their public health significance." Bangladesh Veterinarian 32, no. 1 (2016): 13–18. http://dx.doi.org/10.3329/bvet.v32i1.29252.

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This study determined bacterial quality of chicken rolls sold in a fast food restaurant at Bangladesh Agricultural University (BAU) campus. Fifteen chicken rolls (ten premicrowaved and five post-microwaved) were collected. Samples were inoculated into selective media, Eosin Methylene Blue (EMB) agar, Salmonella Shigella (SS) agar, Thiosulphate Citrate Bile Salts Sucrose (TCBS) agar and Mannitol Salt (MS) agar. The total viable count (TVC) and total Staphylococcal count (TSC) of pre-microwaved samples were 4.4 log CFU/g and 4.2 log CFU/g, respectively. In post-microwaved samples, the TVC and TS

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Panthi, Nirmal, Mukesh Thapa, Rajani Malla, Arun Nagarkoti, Hari Datta Bhattarai, and Babita Paudel. "Oral Microbial Diversity Among Nepalese Individuals Across Various Geographical Regions." Journal of Nepal Biotechnology Association 5, no. 1 (2024): 46–52. http://dx.doi.org/10.3126/jnba.v5i1.63746.

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A comprehensive study encompassed the collection of 153 samples derived from oral patients across 17 diverse locations throughout Nepal. The assortment of samples included extracted teeth, dental plaque, and dental calculus, procured from dental clinics, dental hospitals, and dental camps. Employing six distinct culture media, namely nutrient agar (NA), Muller Hilton agar (MHA), mannitol salt agar (MSA), blood agar (BA), brain heart infusion agar (BHA), and potato dextrose agar (PDA) for potential fungal strains, plates were meticulously incubated at 37°C for 5-7 days. The ensuing bacterial co

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Li, Chuanfeng, Yuanyuan Zhai, and Gailing Wang. "Finding and classifying a microfunctional bacterial strain in fermented sourdoughs." Molecular & Cellular Biomechanics 21 (September 3, 2024): 144. http://dx.doi.org/10.62617/mcb.v21.144.

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The aim of this study was to examine the variety of Lactic acid bacteria and yeasts found in traditionally fermented sourdough, as well as their functional characteristics. Bengal red agar medium, Potato-based liquid media (PDA) medium, and DeMan, Rogosa and Sharpe (MRS) medium were used in that order to isolate, purify, and preserve Saccharomyces cerevisiae and Lactobacillus lactis. In order to analyze single strand breaks, the isolates underwent DNA extraction, Polymerase Chain Reaction (PCR) amplification, and sequencing techniques. The main lactic acid bacteria in the yeast dough were iden

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Kanan, Maria. "Isolasi dan Identifikasi Biokimiawi Bakteri Patogen pada Saluran Pencernaan Lalat Hijau (Chryzomya megachepala)." Jurnal Kesmas Untika Luwuk : Public Health Journal 10, no. 1 (2019): 31–40. http://dx.doi.org/10.51888/phj.v10i1.6.

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Lalat dapat mentransmisikan sekitar 64 jenis bakteri patogen, pada manusia. Genus Musca, Chrysomya dan Sarcophaga merupakan genus lalat, dimana anggota-anggota spesiesnya, banyak berhabitat di sekitar manusia. Chrysomya sp. dikenal sebagai lalat hijau dengan ciri warna tubuh yang hijau atau kehijauan, mengkilat dan berpotensi menimbulkan myasis baik pada manusia maupun hewan. Bakteri Patogen adalah bakteri parasit yang menimbulkan penyakit pada hospes atau inang yang dihinggapi. Tujuan penelitian ini untuk mengetahui jenis bakteri patogen yang terdapat pada saluran pencernaan lalat hijau (C. m

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Chidinma, Nwachukwu, Nwachukwu N. C., Nwankwo E. O., and Nwachukwu E. "Methicillin-Resistant Staphylococcus aureus among Poultry in Umuahia, Nigeria." Microbiology Research Journal International 34, no. 1 (2024): 1–9. http://dx.doi.org/10.9734/mrji/2024/v34i11421.

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Methicillin-resistant Staphylococcus aureus (MRSA) among poultry was investigated. Methicillin-resistant S. aureus (MRSA) poses serious public health issues worldwide and infections resulting from these organisms present difficult therapeutic challenges. A total of 250 swab samples were collected from the skin, cloaca, and egg shells of chickens as well as from feeds, droppings and environment of poultry farms located in rural communities of Umuahia, Nigeria. The respective specimens were inoculated onto mannitol salt agar using the streak plate technique. Inoculated plates were incubated at 3

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Amarantini, Charis, Vinsa Cantya Prakasita, and Maria Trivonia Sema. "Preliminary Selection of Potential Probiotic Lactic Acid Bacteria Isolated from Cocoa Beans (Theobroma cacao)." Biosfer: Jurnal Tadris Biologi 12, no. 1 (2021): 1–8. http://dx.doi.org/10.24042/biosfer.v12i1.7128.

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This study aims to isolate and identify LABs that spontaneously grow fermented cocoa beans that have the potential as probiotics. The isolation was carried out selectively using an MRS agar medium supplemented with 1% CaCO3. LAB's potential as a probiotic was selected based on the acid resistance test, bile salt resistance test, and antimicrobial activity test. The results showed that three isolates of lactic acid bacteria, namely strains K1B1, K2T2, and K3B4 were tolerant of gastric acid's pH conditions (pH 2.5, pH 3, and pH 4). They also can survive the bile salts (0.3%, 0, 5%, and 1%) and h

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Awlachew, Zewdu, Atsede Degefa, Melese Bogale, Moges Birhanu, Desilal Molla, and Gebeyehu Mengstie. "Inoculation of Acinetobacter johnsonii GY08 to enhance the growth of faba bean (Vicia faba L.) under different salt concentrations." Journal of Agricultural Sciences, Belgrade 69, no. 3 (2024): 255–69. http://dx.doi.org/10.2298/jas2403255a.

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Abiotic stresses affect microbial populations and soil chemical and physical properties resulting in significant yield losses of several crops. An important environmental component that impacts plant growth and development from seed germination to maturity is salinity. The objective of this study was to examine the effect of inoculating salt-tolerant rhizobacteria on the morphological and physiological characteristics of faba bean under different salt concentrations in pot experiments. Eight rhizobacterial isolates were tested for their salt tolerance ability on nutrient agar. One best toleran

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Rahma, Mohamed Ibrahem. "Detection of Drug Resistance Staphylococcus Aureus from Sheep Mastitis Milk." South Asian Research Journal of Biology and Applied Biosciences 6, no. 02 (2024): 56–62. http://dx.doi.org/10.36346/sarjbab.2024.v06i02.003.

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In Iraq, sheep play an important role in supplying the local market’s requirements for milk and meat. The objective of this study, 117 milk samples collected from Awassi ewes put to sale in the animal sales yard in Al-Qasim District from Babylon Governorate during four weeks on December 2023. The udder and milk inspection by visual examination and indicator papers (BOVIVET) for detection of mastitis. Consequently, cultured mastitis milk sample on HiCrome™ Klebsiella Selective Agar Base for isolation and easy detection of Klebsiella spp and phenotypic detection of Escherichia coli (EC) on MacCo

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GENIGEORGIS, CONSTANTIN, JORGE H. TOLEDO, and FERNANDEZ J. GARAYZABAL. "Selected Microbiological and Chemical Characteristics of Illegally Produced and Marketed Soft Hispanic-Style Cheeses in California." Journal of Food Protection 54, no. 8 (1991): 598–601. http://dx.doi.org/10.4315/0362-028x-54.8.598.

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Recently, there has been a great concern among California state health authorities and the dairy industry that large volumes of illegally produced Hispanic-style cheeses find their way into local markets. In cooperation with the State Milk and Dairy Foods Control Branch, California Department of Agriculture, 100 samples of soft Hispanic-style cheese were collected from all over the state by undercover agents during seizures by state authorities. The cheeses were analyzed in our laboratory for aerobic plate counts, the presence of Salmonella and Listeria, pH, moisture, and salt (%). The state l

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Umunna, Victoria Chinaza, Sophina Ogonna Umeh, Onyekachukwu Izuchukwu Udemezue, Chibuenyim Ruth Onyekachi, and Chibuike Emmanuel Okafor. "Probiotic Properties of Lactobacilluscasei and L. plantarum Isolated From Overripe Avocado Pear." Biotechnology Journal International 28, no. 5 (2024): 74–83. http://dx.doi.org/10.9734/bji/2024/v28i5743.

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Probiotics have been researched for their benefit to gut health. Research for sources of probiotic bacteria has moved from milk to plants, as they have been found to be an inexhaustible source of lactic acid bacteria (LAB). The probiotic properties of lactic acid bacteria isolated from Over ripe Avocado pear on selected bacterial species were assessed. Fifty Unripe Avocado pears were purchased from different markets and allowed to ferment. Enumeration of the microorganisms and pure culture isolation were done on MRS agar supplemented with1.0% CaCO3 at 37 oC for 48 hours; using the pour plate a

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Klitynska, O. V., T. I. Zorivchak, and R. Y. Krychak. "CHARACTERISTICS OF ROOT CANAL MICROBIOTA IN ACUTE PULPITIS OF TEMPORARY TEETH." Ukrainian Dental Almanac, no. 3 (September 26, 2023): 74–78. http://dx.doi.org/10.31718/2409-0255.3.2023.12.

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Aims. To determine the representatives of the microbiota of the root canals seeded in acute forms of pulpitis of temporary teeth among children of the Transcarpathian region, taking into account the endemic features of the Transcarpathian region and its influence on the course of the carious process. Materials and methods. Collection of biomaterial was carried out using transport tubes with Amies transport medium. The biomaterial was delivered to the laboratory within 2-4 hours after selection. In order to evaluate the microbiota of the root canals, cultivation of biomaterial was carried

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Kunwar, Arzu, Samyukta Tamrakar, Shyaron Poudel, Sony Sharma, and Pramila Parajuli. "Bacteriological Assessment of the Indoor Air of Different Hospitals of Kathmandu District." International Journal of Microbiology 2019 (April 8, 2019): 1–9. http://dx.doi.org/10.1155/2019/5320807.

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Nosocomial infection is the infection that has been caught in a hospital and is potentially caused by organisms that are not susceptible to antibiotics. Nosocomial infections are transmitted directly or indirectly through air and may cause different types of infections. This study was undertaken with an objective to determine the prevalence of nosocomial bacteria present in hospital indoor environment. A total of 16 air samples were taken from general wards and emergency wards of 8 different hospitals using an impactor air sampler in nutrient agar, mannitol salt agar, blood agar, cetrimide aga

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Rengasamy, Sathya, та Ushadevi Thangaprakasam. "ISOLATION, SCREENING AND DETERMINATION OF Α-AMYLASE ACTIVITY FROM MARINE STREPTOMYCES SPECIES". International Journal of Pharmacy and Pharmaceutical Sciences 10, № 4 (2018): 122. http://dx.doi.org/10.22159/ijpps.2018v10i4.24447.

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Objective: This study was aimed to isolate potent amylase producing Streptomyces from the marine source.Methods: Soil samples were collected from less explored mangrove regions of Muthupet, Tamilnadu. Isolation of Streptomyces was performed by serial dilution plate technique using starch casein agar (SCA) (pH 7.2 and temp 28 °C). Morphological and biochemical characteristics were studied using Bergey’s manual of systematic bacteriology. Preliminary screening and quantification of amylase activities were analysed in selected Streptomyces isolates by starch agar plate and dinitrosalicylic acid (

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Favour Okwukweka Nwajiobi, Nwanneka Chinazor Nwozor, Chukwuebuka Chisom Anene, Obumneme Christian Soludo, and Euphemia Afoma Ikegwuonu. "Evaluation of probiotic and antimicrobial properties of Lactobacillus species isolated from decaying Musa acuminata." International Journal of Science and Research Archive 14, no. 2 (2025): 1709–17. https://doi.org/10.30574/ijsra.2025.14.2.0509.

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The increasing interest in probiotics has heightened the need to explore natural sources of beneficial microorganisms. While dairy products have long been a staple for probiotic isolation, plant sources offer a diverse array of novel strains of probiotics with antimicrobial properties. This study aimed to evaluate the probiotic potentials and antimicrobial activity of Lactobacillus species isolated from decaying Musa acuminate (banana). Ten different decaying banana samples were obtained from different banana vendors. Lactobacillus was isolated in deMan Rogosa Sharpe (MRS) agar containing 0.5%

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Rahman, MM, M. Salah Uddin, S. Zaman, et al. "Comparative study on growth and morphological characteristics of a wild type strain Rhizobium spp. (RCA-220) and a genetically engineered E. coli BL21." Journal of Bio-Science 20 (January 18, 2014): 75–82. http://dx.doi.org/10.3329/jbs.v20i0.17718.

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Context: Comparison between a wild type strain Rhizobium spp. (RCA-220) and a genetically modified strain E. coli BL21 in context of growth features. Objective: To observe the comparative growth characteristics of a genetically modified E. coli BL21 and an isolated wild type strain Rhizobium spp. (RCA-220). Materials and Methods: Different kinds of investigations were accomplished in both Luria-Bertani (LB) liquid and semi-solid media to observe the growth and maintenance of these strains. For the isolation of Rhizobium spp. selective Yeast Extract Manitol Agar (YEMA) was used. Colony morpholo

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Jiang, Yi, Yan-Ru Cao, Jutta Wiese, et al. "Streptomyces sparsus sp. nov., isolated from a saline and alkaline soil." International Journal of Systematic and Evolutionary Microbiology 61, no. 7 (2011): 1601–5. http://dx.doi.org/10.1099/ijs.0.020669-0.

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A salt- and alkali-tolerant actinomycete strain, YIM 90018T, was isolated from a saline and alkaline soil sample collected from Qinghai, China. Aerial hyphae of strain YIM 90018T were only produced on YIM 82 agar. Vegetative hyphae were well developed and did not fragment. Straight or flexuous (rectiflexibiles) spore chains were produced. The isolate grew well with 25 % (w/v) MgCl2 . 6H2O and at pH 10. All of these characters indicated that strain YIM 90018T belonged to the genus Streptomyces. On the basis of phylogenetic analysis of the 16S rRNA gene sequence, DNA–DNA hybridization and phenot

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Junaedi, Abdus Salam, M. Atho’illah, M. Atho’illah, et al. "SCREENING ACTIVITY OF LIPOLYTIC BACTERIA: BIOFOULING COMPLEX ON SURAMADU CONCRETE BRIDGE SUBSTRATE." Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology 21, no. 1 (2025): 48–56. https://doi.org/10.14710/ijfst.21.1.48-56.

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Biofouling is an aquatic biota that lives by attaching to a harmful substrate. Bacteria are one of the factors in the formation of biofouling communities on the substrate. Lipolytic bacteria are microorganisms capable of degrading lipid (fat) compounds. The purpose of this study aimed to determine the characteristics of lipolytic bacteria that were successfully isolated from a sample of the Suramadu concrete bridge substrate (Surabaya-Madura waters). There are 3 sampling stations doing 3 repetitions at each station. The isolation method used was a pour plate using selective Stone Mineral Salt

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Devkota, Hari K., Bijaya L. Maharjan, Bikash Baral, Anjana Singh, and Kayo D. Yami. "Invitro Screening of Antifungal Activity of Rhizospheric Bacteria and Possible Role of Chitinase in Antifungal Activity." Nepal Journal of Science and Technology 12 (July 23, 2012): 304–11. http://dx.doi.org/10.3126/njst.v12i0.6517.

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The use of biocontrol agents is becoming an increasingly important alternative to chemical crop protection against weeds, insects and plant diseases in the field of agriculture. The success of biocontrol and yield increase depends on the nature of antagonistic properties and mechanisms of action of the biocontrol agent against the phytopathogens. In this study, 103 macroscopically different bacterial isolates (62 from Kirtipur and 41 from Khumaltar) from 21 different rhizosphere soil samples (11 from Kirtipur and 10 from Khumaltar) were screened for antagonism against five fungal phytopathogen

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Akter, Taslima, Md Shahidur Rahman Khan, Saifur Rahman, Md Ariful Islam, and Md Shafiqul Islam. "Isolation and molecular detection of respiratory bacterial agents from buffalo reared in some selected areas of Bangladesh." Asian Journal of Medical and Biological Research 4, no. 4 (2018): 416–21. http://dx.doi.org/10.3329/ajmbr.v4i4.40115.

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In buffalo respiratory bacterial infection is very common which occurs sporadically or enzootically all over Bangladesh causing high economic loss. The study was performed with a view to proper control of respiratory bacterial infection in Buffalo. A total of 40 samples were collected on the basis of clinical signs. The samples were then subjected to isolation, identification and characterization of the bacterial agents using cultural, biochemical and molecular techniques. Antibiogram profiles of the isolated agents were studied by disc diffusion method. Pasteurella multocida, Staphylococcus a

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Ryman, Valerie E., Felicia M. Kautz, and Steve C. Nickerson. "Case Study: Misdiagnosis of Nonhemolytic Staphylococcus aureus Isolates from Cases of Bovine Mastitis as Coagulase-Negative Staphylococci." Animals 11, no. 2 (2021): 252. http://dx.doi.org/10.3390/ani11020252.

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Staphylococcus aureus is one of the most concerning mastitis-causing pathogens in dairy cattle. Using basic microbiological techniques, S. aureus is typically identified by colony characteristics and hemolysis on blood agar where isolates without hemolysis are typically considered to be coagulase-negative staphylococci (CNS) isolates. Herein, we present a decade-long case study where suspected S. aureus isolates from one Georgia dairy farm were further tested to confirm presumptive identification. Presumptive identification of bacterial growth from 222 mammary secretions from bred Holstein hei

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Ahmed, Zahraa Ayid. "Isolation and diagnosis of Staphylococcus aureus from wounds and detection of some virulence factors." European Journal of Medical Genetics and Clinical Biology 1, no. 5 (2024): 198–205. http://dx.doi.org/10.61796/jmgcb.v1i5.511.

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The current study aimed to isolate and diagnose S. aureus, test its antibiotic susceptibility, and detect some virulence factors. 150 Clinical samples were collected from Kirkuk Hospital in Kirkuk city for the period from December 2023 to March 2024 from patients who were admitted and hospitalized after consulting the specialist doctor and referring him to the laboratory. Colonies of S. aureus were diagnosed based on the culturing characteristics of the colonies growing on mannitol salt agar medium and blood agar media and incubated at 37 0C for 24 hrs. The results showed that 47 (31.3%) of th

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Quiloan, Maria L. G., John Vu, and John Carvalho. "Enterococcus faecalis can be distinguished from Enterococcus faecium via differential susceptibility to antibiotics and growth and fermentation characteristics on mannitol salt agar." Frontiers in Biology 7, no. 2 (2012): 167–77. http://dx.doi.org/10.1007/s11515-012-1183-5.

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Paquette, Sarah-Jo, and Tim Reuter. "Escherichia coli: Physiological Clues Which Turn On the Synthesis of Antimicrobial Molecules." Veterinary Sciences 7, no. 4 (2020): 184. http://dx.doi.org/10.3390/vetsci7040184.

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Zoonotic pathogens, like Shiga toxin-producing Escherichia coli (STEC) are a food safety and health risk. To battle the increasing emergence of virulent microbes, novel mitigation strategies are needed. One strategy being considered to combat pathogens is antimicrobial compounds produced by microbes, coined microcins. However, effectors for microcin production are poorly understood, particularly in the context of complex physiological responses along the gastro-intestinal tract (GIT). Previously, we identified an E. coli competitor capable of producing a strong diffusible antimicrobial with mi

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Ahmed, Monzur Morshed, Md Mizanur Rahaman, Md Nur Hossain, et al. "Charaterization of isolated potential lactobacilli and used as probiotic food." Asian-Australasian Journal of Bioscience and Biotechnology 1, no. 2 (2016): 274–83. http://dx.doi.org/10.3329/aajbb.v1i2.61575.

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This research was carried out to isolate and identify lactobacilli from yogurt and vegetables sample from Dhaka, Bangladesh. Twenty five Lactobacillus strains were isolated and identified as Lactobacillus species based on cultural characteristics, biochemical tests, and sugar fermentation. The results showed only four among twenty five isolates were highly responded to probiotic criteria such as low pH tolerance (2.5), bile salt tolerance (2%), gastric juice tolerance (0.5% bile salt, 0.2% NaCl, 0.32% pepsin pH 2.5) in-vitro and were identified strains as Lactobacillus plantarum imy-21, Lactob

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Azmi, Siti Khadijah, Nur Zahra Zainal Fuadi, and Ilyanie Yaacob. "Isolation and Identification of Halophilic Bacteria Isolated from Mangrove Soil in Blue Lagoon, Port Dickson, Negeri Sembilan." Malaysian Journal of Fundamental and Applied Sciences 20, no. 4 (2024): 902–13. http://dx.doi.org/10.11113/mjfas.v20n4.3179.

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Halophiles bacteria are capable of tolerating and surviving in extreme salinity due to the special biological structure that it possesses. Classification of halophilic bacteria includes slight, moderate, and extreme halophiles which range from low to high concentrations of salt. Biotechnological products yielded from bacteria residing in saline environment are proven to be valuable in a few industries. The isolated halophilic bacteria may yield powerful biomolecules such as enzymes which can enhance plant development, hence boosting agricultural industries. However, the insufficient data relat

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Hameed, Rana A. "Physiological and Molecular Assessment of Sesbania Root Nodules Bacteria from Different Iraqi Areas for Salt Tolerance." Biomedical and Pharmacology Journal 12, no. 1 (2019): 391–98. http://dx.doi.org/10.13005/bpj/1652.

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Thirty two isolates of aerobic gram-negative bacteria associated with Sesbania sesban grown in different saline Iraqi soils was identified according to morphological and physiological characteristics, cultured on yeast-mannitol agar medium (YEMA) supplemented with different NaCl concentrations. It was indicated that 53.12% of isolates were highly tolerant to salinity, tolerated from 4.0 to 5.0 w/v NaCl. All thirty two Rhizobia isolates performed positive strong reaction to Catalase enzyme except for three were negative to this enzyme. Concerning exo-polysaccharide (EPS) production the isolates

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Mahgoub, Samir A., Shaza Y. A. Qattan, Salma S. Salem, et al. "Characterization and Biodegradation of Phenol by Pseudomonas aeruginosa and Klebsiella variicola Strains Isolated from Sewage Sludge and Their Effect on Soybean Seeds Germination." Molecules 28, no. 3 (2023): 1203. http://dx.doi.org/10.3390/molecules28031203.

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Phenols are very soluble in water; as a result, they can pollute a massive volume of fresh water, wastewater, groundwater, oceans, and soil, negatively affecting plant germination and animal and human health. For the detoxification and bioremediation of phenol in wastewater, phenol biodegradation using novel bacteria isolated from sewage sludge was investigated. Twenty samples from sewage sludge (SS) were collected, and bacteria in SS contents were cultured in the mineral salt agar (MSA) containing phenol (500 mg/L). Twenty colonies (S1 up to S20) were recovered from all the tested SS samples.

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Hien, Pham Hong, Tran Van Mau, Nguyen Thanh Huyen, et al. "Impacts of Culture Conditions on Ligninolytic Enzymes (LIP, MNP, and Lac) Activity of Five Bacterial Strains." Vietnam Journal of Agricultural Sciences 3, no. 1 (2020): 520–29. http://dx.doi.org/10.31817/vjas.2020.3.1.05.

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In this study, with the aim of determining and assessing the influence of several culture conditions on the ligninolytic enzyme (LiP, MnP, and Lac) activity of bacteria, five lignin-degrading bacteria strains were isolated from two different soil samples and cultured on minimum salt medium agar containing alkaline lignin (MSML agar). Among the five isolated strains, DL1 and X3 expressed strong and stable ligninase enzyme activity at various temperature levels (30°C, 37°C, 50°C, and 60°C) and were selected for further study. Notably, at 60°C, the ligninase activity of both strains lasted until

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Saha, Tapu Kumar, Ferdousi Begum, SM Lutful Kabir, Md Shafiqul Islam, and Md Shahidur Rahman Khan. "Characterization of bacterial isolates from skin lesions of sheep, goat and cattle in different rearing condition." Asian Journal of Medical and Biological Research 5, no. 2 (2019): 117–25. http://dx.doi.org/10.3329/ajmbr.v5i2.42493.

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The study was performed to isolate and characterize some selected bacteria from skin lesion of sheep, goat and cattle of different rearing condition. Intensive and semi-intensive housing systems were considered for this study. A total of (n=60) skin wound swab and pus samples were collected for this study during the study period. Among them (n=30) samples were collected from intensive farms and (n=30) samples were collected from semi-intensive farms. Staphylococcus aureus infections were found 70% skin lesion for sheep, 30% for goat and 60% for cattle and Pseudomonas aeruginosa infections were

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Bello, Olorunjuwon O., Edward O. Oyekanmi, Babatunde A. Kelly, Olakunle O. Mebude, and Temitope K. Bello. "Antibiotic Susceptibility Profiles of Bacteria from Diabetic Foot Infections in Selected Teaching Hospitals in Southwestern Nigeria." International Annals of Science 4, no. 1 (2018): 1–13. http://dx.doi.org/10.21467/ias.4.1.1-13.

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One of the serious complications associated with diabetes is foot ulcer and this condition affects the quality of life in patients in all classes, races and ages. Chronic wounds are prone to colonization by wide array of microorganisms which could be extremely hazardous to patients if effective and timely therapeutic intervention is not made. This study was conducted to determine the antibiotic susceptibility profiles of bacteria from diabetic foot infections in southwestern Nigeria. Samples were collected from a total of 142 diabetic foot ulcer patients with moistened sterile cotton swabs. Nu

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Chowdhury, Md. Estiak Khan, Arnaba Saha Chaity, Alam Khan, et al. "Molecular characterization of the pathogen responsible for Choanephora fruit rot disease in Momordica charantia (L.) and establishment of its ecofriendly control measures." GSC Biological and Pharmaceutical Sciences 11, no. 3 (2020): 022–33. https://doi.org/10.5281/zenodo.4271174.

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The present investigation was carried out to characterize the pathogen responsible for <em>Choanephora </em>fruit rot disease in bitter gourd along with find out appropriate control measure using biological resources. The pathogenic fungal strain was isolated from the disease infected fruit part of bitter gourd. It was subjected to four different types of growth media named potato dextrose agar (PDA), Czapeck Dox agar (CDA), nutrient agar (NA) and Sabouraud dextrose agar (SDA) were used to note down the differences regarding to various characteristics appeared due to dissimilation of

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R, Kushbu. "Proportional Analytics of Test Samples Reported with Urinary Tract Infection." Clinical Research and Studies 2, no. 1 (2023): 01–07. https://doi.org/10.31579/2835-2882/009.

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Urinary tract infection (UTI) has been reported most often in recent days. In a clinical aspect there are various reasons for this cause. Out of 100 samples tested (Clinical samples from Rashi Diagnostic Center- Bangalore NABL laboratory), under the analytical aspect several comparative analysis was done. Urine was tested within half an hour of the collection. Physical characteristics of Urine sample were studied initially. Laura M is the device used to analyses the Uro-dip. Sugar and the presence of albumin was also found. Sediment of the centrifuged sample was subjected to gram staining and

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Dessie, Tewodros, Mohabaw Jemal, Minwuyelet Maru, and Moges Tiruneh. "Multiresistant Bacterial Pathogens Causing Bacterial Pneumonia and Analyses of Potential Risk Factors from Northeast Ethiopia." International Journal of Microbiology 2021 (March 8, 2021): 1–9. http://dx.doi.org/10.1155/2021/6680343.

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Background. Pneumonia is the most common cause of morbidity and mortality in developing countries, mostly caused by different species of bacterial pathogens. Hence, patient management needs awareness of the pathogens and antimicrobial susceptibility testing (AST). This study was aimed to assess the type of bacterial isolates and their antimicrobial susceptibility patterns among pneumonia suspected patients at Dessie Referral Hospital, Northeast Ethiopia. Potential risk factors were also assessed to apply preventive measures accordingly. Materials and Methods. A cross-sectional study design was

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Haryogya, Aziza Mahdania, and Ustadi. "Isolation and Molecular Identification of Chitinolytic Bacteria from Ronto." E3S Web of Conferences 147 (2020): 03030. http://dx.doi.org/10.1051/e3sconf/202014703030.

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Ronto is a traditional food from South Kalimantan made of processed rebon shrimp. The production of Ronto relies on the fermentation process done by various types of microorganisms, especially bacteria. With shrimp as the main ingredient, Ronto has a high salt concentration and low pH level. Therefore, there is a potential in the exploration of chitinolytic bacteria with specific characteristics in Ronto. This study aimed to isolate and identify chitinolytic bacteria in Ronto. Ronto that was used in this experiment had a pH of 5. The isolation of chitinolytic bacteria using chitin agar obtaine

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Saif, Kazi Md, Ferdousi Begum, Saifur Rahman, Md Shafiqul Islam, KHM Nazmul Hussain Nazir, and Md Shahidur Rahman Khan. "Molecular identification and antibiogram profiles of respiratory bacterial agents isolated from cattle reared in some selected areas of Mymensingh division, Bangladesh." Asian-Australasian Journal of Bioscience and Biotechnology 4, no. 1 (2019): 60–66. http://dx.doi.org/10.3329/aajbb.v4i1.64949.

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Respiratory bacterial infections in cattle are very common all over Bangladesh causing high economic loss. This research was performed with a view to proper control of respiratory bacterial infections of cattle in Bangladesh. A total of 100 nasal samples were collected on the basis of clinical signs. From the collected samples isolation, identification and characterization of the bacterial agents was done using cultural, biochemical and molecular techniques. Antibiogram profiles of the isolated agents were studied by disc diffusion method. Pasteurella multocida, Staphylococcus aureus and Esche

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Srivastava, Amrisha, Puneet Singh Chauhan, and Rachana Singh. "Characterization of Stress-Tolerant Bacteria for the Biosynthesis of Silver Nanoparticles and their Applications." Journal of Nano Research 68 (June 29, 2021): 70–80. http://dx.doi.org/10.4028/www.scientific.net/jnanor.68.70.

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The biogenesis of silver nanoparticles by microbes has become an essential branch in the field of nanotechnology because of its safe, environment-friendly, economical, and time-saving nature. In the current research work, we have screened some stress-tolerant bacteria based on pH, temperature, salt-tolerant efficacy and further utilized them for the synthesis of silver nanoparticles. The test bacterium was isolated from the soil sample through the serial dilution method on nutrient agar media (NAM). Based on identification using morphological characteristics, biochemical analysis, and 16srRNA

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Kurahashi, Midori, та Akira Yokota. "Agarivorans albus gen. nov., sp. nov., a γ-proteobacterium isolated from marine animals". International Journal of Systematic and Evolutionary Microbiology 54, № 3 (2004): 693–97. http://dx.doi.org/10.1099/ijs.0.02778-0.

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Six bacterial strains were isolated from healthy marine organisms that were collected from the coast of the Kanto area in Japan. Phylogenetic analysis based on 16S rDNA sequence similarity showed that the six isolates formed a separate cluster in the γ-Proteobacteria and were related to the genera Alteromonas and Glaciecola (<91·6 % similarity). The isolates were related closely to each other (DNA–DNA reassociation values of 74–93 %). The isolates had a polar flagellum and were Gram-negative, mesophilic, strictly aerobic rods that required salt for growth. Distinct phenotypic features of th

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Aiello, Caterina, Cinzia Quattrocchi, Rosario Musumeci, Daria Nicolosi, Giulio Petronio Petronio, and Roberto Di Marco. "In Vitro Preliminary Evaluation of a New Rifamycin In Situ Gelling Formulation for Pouchitis Treatment." Microbiology Research 16, no. 2 (2025): 44. https://doi.org/10.3390/microbiolres16020044.

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Pouchitis is one of the most common long-term complications after ileal pouch–anal anastomosis (IPAA) surgery, with a reported incidence rate of up to 50%. Identifying specific bacteria involved in the pathogenesis has important implications for clinical management. Indeed, antibiotic therapy is a common treatment option, but antibiotic choice and treatment duration can vary depending on the severity of symptoms and the bacteria involved. Ansamycins are effective in the management of antibiotic-dependent pouchitis. Therefore, this study aimed to test the in vitro antibacterial activity of a no

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Rahaman, M. Mizanur, M. Nur Hossain, Nantu Chandra Das, Monzur Morshed Ahmed, and M. Mahfuzul Hoque. "Potential Thermotolerant Lactobacilli Isolated from Chicken Gastrointestinal Tract for Probiotic Use in Poultry Feeds." Bangladesh Journal of Microbiology 36, no. 2 (2020): 63–68. http://dx.doi.org/10.3329/bjm.v36i2.45529.

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This research was carried out to isolate and identify thermotolerant lactobacilli from broiler chicken gastrointestinal tract from Dhaka, Bangladesh. Twenty five thermotolerant Lactobacillus strains were isolated and identified as Lactobacillus species based on cultural characteristics, biochemical tests, and sugar fermentation. The probiotic are a live microbial feed supplements which positively affects the health of the host animal by improving its intestinal balance.The results showed only four among twenty five isolates were highly responded to probiotic criteria such as low pH tolerance (

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Abbas, Asma, Rabia Kanwar, B. Aslam, et al. "Determination of antimicrobial, pH, bile salt, and gastric juice tolerance properties of Lactobacilli isolated from human milk." International Food Research Journal 28, no. 2 (2021): 302–8. http://dx.doi.org/10.47836/ifrj.28.2.10.

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Human milk is a complex biological fluid, and a source of nutrients and probiotics which plays a vital role in the growth and development of infants. Probiotics are helpful bacteria which are good for human health. The primary bacterial genera which are gaining status as probiotics are Lactobacillus, Lactococcus, and Bifidobacterium. The present work aimed to isolate, identify, and determine the probiotic potential of Lactobacillus bacteria from human milk. A total of 70 samples of human milk were collected from different lactating mothers. The milk samples were inoculated on the De Man, Rogos

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Tinrat, Sirikhwan, and Saranya Sedtananun. "NOVEL RUMMELIIBACILLUS SP. ISOLATED FROM FERMENTED VEGETABLE PRODUCTS AS THE POTENTIAL PROBIOTICS." Journal of microbiology, biotechnology and food sciences 11, no. 5 (2022): e4194. http://dx.doi.org/10.55251/jmbfs.4194.

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The novel lactic acid bacteria has potential probiotics which were selectively screened from different fermented vegetable products by antagonistic activity against Escherichia coli ATCC 25922 as a food-borne pathogenic bacteria. From 40 isolates on MRS agar, some isolates had anti-E. coli activity. Surprisingly, isolated STR 0103f and STR 0404f strains demonstrated bile salt-tolerance at 0.3% bile salt for 24 hours with survival rate of 67.77±0.38 and 68.72±0.45%, respectively. Both strains also survived under acidic conditions (pH 3-5) with 60.53±0.08-76.87±0.09% of survival rate. STR 0103f

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Ghorban Hosseini, Nazila, Mohammad Hossein Modarressi, Seyed Naser Mousavi, and Maryam Tajabadi Ebrahimi. "Evaluation of Novel Probiotic Bacillus Strains Based on Enzyme Production and Protective Activity Against Salmonellosis." Journal of the Hellenic Veterinary Medical Society 69, no. 4 (2019): 1205. http://dx.doi.org/10.12681/jhvms.16089.

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Probiotic strains of Bacillus Species are used in industrial poultry production because of their ability to produce enzymes enhancing the absorption of food, and their ability to reduce the risk of Salmonella spp. infection. The aim of this study was to isolate native potential probiotic Bacillus spp. with the ability to produce enzymes and attachment to intestinal epithelial cells in order to prevent Salmonella typhimurium infection. First, 25 samples of chicken feces were collected from 7 industrial poultry in Golestan province located in north of Iran. Bacillus species from samples were iso

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Lafta, Inam Jasim, and Mohammed Jasim Najem. "Characterization of Mannitol Fermenter and Salt Tolerant Staphylococci from Breast Tumor Biopsies of Iraqi Women." Baghdad Science Journal 17, no. 2 (2020): 0415. http://dx.doi.org/10.21123/bsj.2020.17.2.0415.

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The emergence of staphylococci, either coagulase negative (CNS) or coagulase positive (CPS), as important human pathogens has implied that reliable methods for their identification are of large significance in understanding the diseases caused by them. The identification and characterization of staphylococci from biopsies taken from human breast tumors is reported here. Out of 32 tissue biopsies, a total of 12 suspected staphylococci grew on mannitol salt agar (MSA) medium, including 7 fermenters and 5 non-fermenter staphylococci based on traditional laboratory methods. Polymerase chain reacti

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