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Dissertations / Theses on the topic 'Chemical cross-linking'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Macedo, Georgia Valente de Yamauchi Mitsuo. "Effect of chemical cross-linking agents on dentin bonding." Chapel Hill, N.C. : University of North Carolina at Chapel Hill, 2008. http://dc.lib.unc.edu/u?/etd,1756.

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Thesis (M.S.)--University of North Carolina at Chapel Hill, 2008.<br>Title from electronic title page (viewed Sep. 16, 2008). "... in partial fulfillment of the requirements for the degree of Master of Science in the Department of Operative Dentistry - School of Dentistry." Discipline: Operative Dentistry; Department/School: Dentistry.

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Schulz, Daniela M. "Protein - protein interaction studies by chemical cross-linking and mass spectrometry." lizenzfrei, 2007. http://sundoc.bibliothek.uni-halle.de/diss-online/07/07H316/index.htm.

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3

Lin, De. "Modification and Cross-Linking of Proteins by Lipoxidation Derived Electrophiles: Chemical and Biological Consequences." online version, 2007. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=case1166813930.

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4

Park, Geunhyung. "Characterization of actuator behaviors of ferrogels obtained from physical and chemical cross-linking methods." [Ames, Iowa : Iowa State University], 2008.

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5

Chen, Zhuo A. "3D proteomics : analysis of proteins and protein complexes by chemical cross-linking and mass spectrometry." Thesis, University of Edinburgh, 2011. http://hdl.handle.net/1842/8757.

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The concept of 3D proteomics is a technique that couples chemical cross-linking with mass spectrometry and has emerged as a tool to study protein conformations and protein-protein interactions. In this thesis I present my work on improving the analytical workflow and developing applications for 3D proteomics in the structural analysis of proteins and protein complexes through four major tasks. I. As part of the technical development of an analytical workflow for 3D proteomics, a cross-linked peptide library was created by cross-linking a mixture of synthetic peptides. Analysis of this library

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Makarov, Alexandr. "New insights into the structure and assembly of nuclear lamins from chemical cross-linking and mass spectrometry." Thesis, University of Edinburgh, 2017. http://hdl.handle.net/1842/28872.

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Now that the functioning of microtubules and the actin cytoskeleton has been worked out in enormous detail, the next important task is defining the structure of intermediate filaments that are far behind the other two major skeletal networks due to their inherent resistance to most structural techniques. The evolution of novel structural approaches for flexible proteins is making this possible now. In my thesis I will aim to elucidate the structure and assembly principles of lamin A nuclear intermediate filament protein. To study lamin A, I principally employed chemical cross-linking that allo

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Mudd, Gemma Elizabeth. "Dyes, linkers, tags and libraries : new tools for systems chemical biology." Thesis, University of Edinburgh, 2015. http://hdl.handle.net/1842/17882.

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Chemical biology can be defined as the area of science where chemical tools are used to study biological systems. The simplest way this can be achieved is in the identification of compounds which inhibit or modulate a biological pathway and the consequences studied. However, novel tools are required to enable, for example, the development of assays to allow simpler screening of difficult targets such as membrane proteins and protein-protein interactions. A series of kisspeptin analogues were synthesised for the development of a screening platform compatible with G-protein coupled receptors and

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Jeong, Woncheol 1973. "Studies fo the microstructure of self-assembling peptides : effects of chemical cross-linking and growth characterization." Thesis, Massachusetts Institute of Technology, 2004. http://hdl.handle.net/1721.1/30314.

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Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, February 2005.<br>Includes bibliographical references (p. 32-33).<br>Peptide is a b iomaterial with great promise in tissue engineering because it has known to be capable of self-assembly. Also, the peptide scaffolds can support neuronal cell attachment, differentiation, and neuron outgrowth. They might therefore p lay a role as substrate for functional synapse formation between neurons. One important feature of peptide gel is that its modulus can modulate cell motility in combination with the observation th

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Henry, Nicolas. "A new model of lipidated apoE provided by chemical cross-linking/mass spectrometry and in silico modeling." Doctoral thesis, Universite Libre de Bruxelles, 2014. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/209192.

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Apolipoprotein E (apoE) is crucial for lipid transport and cholesterol homeostasis within the plasma and central nervous system. Binding to lipoprotein particles activates apoE allowing it to interact with cell surface receptors such as the low density lipoprotein receptor (LDLr). This mediates the clearance of apoE containing lipoproteins through an endocytosis pathway and therefore reduces plasma cholesterol level, explaining the strong anti-atherogenic effect of apoE. Nevertheless, the active conformation of apoE, critical for the receptor interaction, remains elusive. Since high resolution

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Anyango, Joseph Ochieng. "Physico-chemical modification of kafirin microstructures for application as biomaterials." Thesis, University of Pretoria, 2012. http://hdl.handle.net/2263/29708.

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Microparticles produced from kafirin, the sorghum grain prolamin protein, by molecular selfassembly using coacervation with acetic acid solvent are vacuolated. They have shown considerable potential for encapsulation of antioxidants and for preparation of high quality free-standing bioplastic films. However, the functional quality of these kafirin microstructures needs to be improved to exploit their potential application, particularly as biomaterials. Wet heat, transglutaminase and glutaraldehyde treatments were used to modify the physical structure and chemical properties of the kafirin micr

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Zheng, Qiuling. "Analysis of Protein Three-Dimensional Structures and Capture of Organic Reaction Intermediates by Mass Spectrometry." Ohio University / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1457995358.

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Mahendran, Ramani Salmalee. "Characterisation of cross-linking and moisture ingress detection in an epoxy/amine resin using fibre-optic sensors." Thesis, University of Birmingham, 2010. http://etheses.bham.ac.uk//id/eprint/1083/.

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Themosetting resins are being used increasingly as matrix materials in advanced fibre-reinforced composites. It is well known that the processing conditions used to manufacture the composite can have a major influence on its mechanical integrity in service. In addition, the processing parameters can also affect the ingress of moisture in thermosetting resin and composites. The feasibility of using optical fibre-based sensor systems for monitoring cross-linking reaction has been demonstrated previously. However, little work has been reported on the deployment of a single sensor to study the cro

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Brittin, Mark. "Analytical and morphological studies of polymer-stabilised liquid crystals." Thesis, University of Reading, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.297785.

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Pettersson, David. "Towards stimuli-reformable paperboard and flipped classroom in chemistry education : A study of how to create a paper with controllable mechanical properties and a study in how the flipped classroom is used in chemistry education." Thesis, KTH, Skolan för teknikvetenskaplig kommunikation och lärande (ECE), 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-218299.

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Today, paper and paperboard is an important part of the packaging industry, as well as natural part of our daily lives. The alternatives to paper and paperboard is often produced from nonrenewable sources such as petroleum and as demands on sustainability increase in the packaging sector, it gives paper a desirable advantage over plastics. To increase the use of paper, some of the limiting factors for paper needs to be overcome, such as its limited formability. One way to create a paper that can be formed in new ways is to imbibe the paper with an additive that makes its modulus changeable. Th

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Perkins, Brittany. "PROBING GAS-PHASE PEPTIDE STRUCTURE AND PROTEIN-PROTEIN INTERACTIONS USING MASS SPECTROMETRIC TECHNIQUES." Diss., The University of Arizona, 2009. http://hdl.handle.net/10150/194317.

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Presented in this dissertation are studies on the gas-phase structural features of peptides and peptide fragment ions using mass spectrometry (MS), hydrogen/deuterium (H/D) exchange, infrared multiphoton dissociation (IRMPD) spectroscopy, and computational modeling. Additional studies are presented on the mechanism of hydrogen/deuterium exchange using a model amino acid system. The application of chemical cross-linking to investigate the interaction between two proteins, LexA and RecA, is also presented. Gas-phase structural features can be probed using a number of techniques, and several of t

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Bajwa, Amrita Kaur. "The effect of chemical and light cross-linking procedures on the levels of beneficial growth factors in human amniotic membrane for use in ophthalmology." Thesis, Boston University, 2011. https://hdl.handle.net/2144/34438.

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Thesis (M.A.)--Boston University<br>PLEASE NOTE: Boston University Libraries did not receive an Authorization To Manage form for this thesis or dissertation. It is therefore not openly accessible, though it may be available by request. If you are the author or principal advisor of this work and would like to request open access for it, please contact us at open-help@bu.edu. Thank you.<br>The overall goal of this research is to alleviate suffering and improve visual outcomes for burn patients during recovery, using amniotic membrane as a stabilized 'patch' for the corneal surface. Second and th

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Kumar, Lalit. "DEVELOPMENT OF NOVEL CHEMICAL TOOLS FOR PROTEASOME BIOLOGY & A NEW APPROACH TO 1-AZASPIROCYCLIC RING SYSTEM." UKnowledge, 2012. http://uknowledge.uky.edu/chemistry_etds/14.

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The proteasome, a multiprotease complex, is clinically validated as an anticancer target by the FDA approval of bortezomib and carfilzomib for the treatment of multiple myeloma. The emergence of resistance to proteasome inhibitors however remains a major clinical challenge. Recently, distinct types of proteasomes termed ‘intermediate proteasomes’, which contain unconventional mixtures of catalytic subunits, have been implicated with drug resistance of tumor cells. In elucidating the role of intermediate proteasomes in drug resistance, a crucial step is to unequivocally determine the subunit co

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TERENCE, MAURO C. "Obtencao de um sistema de liberacao controlada de drogas a partir do PVAL irradiado com radiacao gama." reponame:Repositório Institucional do IPEN, 2002. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10984.

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WINTERS, MICHAEL SHAWN. "PROBING PROTEIN-PROTEIN INTERACTIONS in vitro and in vivo WITH CYANOGEN." University of Cincinnati / OhioLINK, 2002. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1027090541.

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Schiffner, Torben. "Refocusing antibody responses by chemical modification of vaccine antigens." Thesis, University of Oxford, 2014. http://ora.ox.ac.uk/objects/uuid:2b2cac0f-6be3-4f91-96e5-9888e02780d4.

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The envelope glycoprotein (Env) of Human Immunodeficiency Virus 1 (HIV-1) has developed several immune-evasion mechanisms to avoid the induction of neutralising antibodies, including immunodominant non-neutralising epitopes, conformational flexibility of conserved epitopes, and spontaneous subunit dissociation, thus impeding vaccine development. Here, chemical modification of Env-based vaccine antigens is explored to overcome these obstacles. Firstly, covalent fixation of Env by chemical cross-linking was used to stabilise the conformationally flexible structure and prevent subunit dissociatio

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D'angelo, Anthony Joseph. "Investigation and Synthesis of Novel Graphene-Based Nanocomposites for Hydrogen Storage." Scholar Commons, 2012. http://scholarcommons.usf.edu/etd/4024.

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It is of great interest to develop and utilize a high surface area material with optimized hydrogen sorption properties. The need for a renewable energy source to replace automobile gasoline has become more critical in the past decade. Hydrogen is a viable fuel source for automobile usage; however, the question of how hydrogen will be safely and efficiently stored still remains. Critical factors for optimum hydrogen storage include ambient conditions and low activation temperature for adsorption and desorption phenomena. In order for optimum hydrogen adsorption to be achieved, the properties o

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Cravello, Laëtitia. "Etudes structurales des protéines par spectrométrie de masse couplée aux échanges hydrogène/deuterium et à la réticulation chimique." Université Joseph Fourier (Grenoble), 2005. https://tel.archives-ouvertes.fr/tel-00009698.

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Les protéines sont impliquées dans de nombreux processus biologiques. Il est nécessaire pour comprendre en détail leur fonction et leur mode d’action afin d’obtenir des informations sur leur structure et sur leurs interactions éventuelles avec leurs partenaires. Le travail réalisé durant cette thèse a consisté à développer deux méthodes innovantes utilisant la spectrométrie de masse pour étudier la structure des protéines et à appliquer ces méthodes à une problématique biologique. Nous avons optimisé une méthode associant les échanges H/D et la spectrométrie de masse sur une protéine modèle, l

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EVORA, MARIA C. C. "Estudo do efeito da radiacao sobre a poliamida-6 reciclada." reponame:Repositório Institucional do IPEN, 2001. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10958.

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Fux, Anja [Verfasser], Stephan A. [Akademischer Betreuer] Sieber, Matthias J. [Gutachter] Feige, and Stephan A. [Gutachter] Sieber. "ClpXP Protease Networks studied by Chemical Cross-Linking Combined with Mass Spectrometry – A Proteomic Survey on Human Pyridoxal 5’-Phosphate-Binding Proteins / Anja Fux ; Gutachter: Matthias J. Feige, Stephan A. Sieber ; Betreuer: Stephan A. Sieber." München : Universitätsbibliothek der TU München, 2019. http://d-nb.info/1200547861/34.

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Sabzalian, Zohreh. "Cross-linking and hydrophobization of chemically modified cellulose fibers." Thesis, McGill University, 2013. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=114269.

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Owing to its unique structure, along with the inexhaustible renewability, cellulose has been a subject of scientific and commercial interest for over 150 years. However, given attractive structural properties, such as stiffness, hydrophilicity, stereoregularity, potential for chemical modifications and ability to form superstructures, utilization of this biopolymer is far below its potential. Over the past few years the number of research projects to modify cellulose fibers to make them more suitable for numerous applications has increased. Transforming hydrophilic cellulose fibers into hydrop

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Debnárová, Simona. "Modifikace termocitlivého kopolymeru bioaktivními látkami pro medicínské aplikace." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2018. http://www.nusl.cz/ntk/nusl-438887.

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Biodegradabilné syntetické polyméry nesú vlastnosti, ktoré ich zvýhodňujú oproti iným materiálom používaným na poli regeneratívnej medicíny a tkanivového inžinierstva. Najdôležitejšie výhody zahŕňajú schopnosť prispôsobovať mechanické a chemické vlastnosti aj kinetiku degradácie. Obzvlášť polyestery sú zaujímavé z pohľadu na ich biodegradáciu. Podliehajú hydrolýze, počas ktorej dochádza k štiepeniu esterových väzieb a degradačné produkty sú metabolizované bez akýchkoľvek škodlivých účinkov. Diplomová práca je zameraná na syntetické biodegradabilné triblokové kopolyméry PLGA-PEG-PLGA s obsahom

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Bendifallah, Maya. "Development of Inhibitors of Amyloid Fibril Propagation." Thesis, Université Paris-Saclay (ComUE), 2019. http://www.theses.fr/2019SACLS578.

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L'α-Synuclein (αSyn) fibrillaire, impliqué dans la maladie de Parkinson et d’autres synucleinopathies, peut se propager entre cellules de manière « prion-like » et cette propagation est liée à la progression de la maladie. Durant cette étude, nous nous sommes tournés vers les chaperons moléculaires impliqués dans l’agrégation de l’αSyn ou bien dans sa toxicité afin de trouver des candidats capables d’interférer avec la propagation. Nous avons ensuite testé l’effet des chaperons capables de se lier aux fibres d’αSyn sur l’internalisation des fibres d’αSyn par les cellules Neuro-2a. Nous démontr

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Shukri, Radhiah. "Effects of amylose content and chemically cross-linking starch on in-vitro digestibility and extrusion of starch." Diss., Kansas State University, 2013. http://hdl.handle.net/2097/15636.

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Doctor of Philosophy<br>Department of Grain Science and Industry<br>Yong Cheng Shi<br>This study focused on in-vitro digestibility and pasting properties of cross-linked (CL) starch with different amylose contents and different cross-linking levels, as well as physicochemical properties of extruded wheat flours with different amylose contents and functionality of amylopectin and cross-linking in improving the textural and physical properties of oat flour extrudates. Starch was CL by phosphorylation using a mixture of sodium trimetaphosphate and sodium tripolyphosphate at the ratio of 99:1 unde

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Silva, Mojica Ernesto. "Polymer-silica Hybrids for Separation of CO2 and Catalysis of Organic Reactions." University of Akron / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=akron1398439043.

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HUEY, WONG SHENQ, and 黃聖惠. "Mapping Amino Acid Proximities in Sup35 Prion Strains by Chemical Cross-Linking." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/35422132030201157256.

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博士<br>國防醫學院<br>生命科學研究所<br>103<br>Strains of the yeast prion [PSI] are different folding patterns of the same Sup35 protein, which stacks up periodically to form a prion fiber. Chemical cross-linking is employed here to probe different fiber structures assembled with a mutant Sup35 fragment. The photo-reactive cross-linker, p-benzoyl-ʟ-phenylalanine (pBpa), was biosynthetically incorporated into bacterially prepared recombinant Sup(1-61)-GFP, containing the first 61 residues of Sup35, followed by the green fluorescent protein. Four methionine (Met) substitutions and two alanine (Ala) substituti

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Yiu, Pei-Chun, and 游珮均. "Analysis of protein organization of the postsynaptic density by chemical cross-linking method." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/07671344073332357866.

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Lin, Tien-Chen, and 林天正. "Chemical Cross-linking and Mass Spectrometric Analysis of Human Mitochondrial Malic Enzyme Structure." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/92687538536499595120.

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碩士<br>國立陽明大學<br>生化暨分子生物研究所<br>94<br>Human mitochondrial NAD+-dependent malic enzyme (m-NAD-ME) plays an essential role in glutamate metabolism for energy generation in rapidly proliferating tissues and tumors. Its structure is a double dimer composed of four 65-kDa subunits. Based on current X-ray crystal structures of m-NAD-ME with different binding modes, it was proposed that m-NAD-ME may exert conformational transformation upon the binding of cofactors, activator and substrate. However, such proposed structure changes have not been completely verified and many questions remained to be answe

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Schulz, Daniela M. [Verfasser]. "Protein - protein interaction studies by chemical cross-linking and mass spectrometry / von Daniela M. Schulz." 2007. http://d-nb.info/990264017/34.

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Chang, Chia-Wei, and 張家瑋. "Studying the protein-protein interactions in the postsynaptic density by immunoabsorption and chemical cross-linking means." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/37473295688331306626.

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Honzejková, Karolína. "Biofyzikální charakterizace N-koncové části proteinkinasy ASK1." Master's thesis, 2019. http://www.nusl.cz/ntk/nusl-396776.

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Apoptosis signal-regulating kinase 1 (ASK1) is an apical kinase of the mitogen-activated protein kinase cascade. Its activity is triggered by various stress stimuli such as reactive oxygen species (ROS), cytokines, endoplasmic reticulum (ER) stress or osmotic stress resulting in the activation of p38 and c-Jun N-terminal kinase metabolic pathways and leading to inflammation or cell death. Dysregulation of ASK1 is linked to several pathologies such as neurodegenerative and cardiovascular diseases and cancer, which makes this protein a potential target of therapeutic intervention. The activity o

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Shu-WeiWu and 吳書瑋. "Strengthening injectable thermo-sensitive NIPAAm-g-chitosan hydrogels by chemical cross-linking as scaffolds for cartilage tissue engineering." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/5gr8uq.

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Vaňková, Pavla. "Strukturní charakterizace intracelulární formy myšího Nkr-p1a proteinu." Master's thesis, 2016. http://www.nusl.cz/ntk/nusl-343063.

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NK cells are a component of innate immunity system, which is derived from lymphoid progenitor. By a sophisticated receptor repertoire, which is expressed on their surface, they provide a surveillance against pathogenic, virus infected or tumour cells. Simultaneously they produce cytokines, thereby are involved in adaptive immune response. This work is focused on the study of structure of mice soluble mNkr-p1a isoform. Recently this short isoform was identified at the transcriptional level by a member of our laboratory and it is designated as isoform 2. The aim was to produce mNkr-p1a iso2 prot

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Kukačka, Zdeněk. "Výzkum Struktury β-N-Acetylhexosaminidasy z Aspergillus oryzae". Master's thesis, 2011. http://www.nusl.cz/ntk/nusl-298082.

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in English β-N-acetylhexosaminidase (EC 3.2.1.52) belongs to exoglycosidase, and is one of the most abundant enzymes found in organisms from bacteria to human. The fungal β-N-acetylhexosaminidase from Aspergillus oryzae is composed of propeptide and catalytic domain. The propeptide is noncovalently associated with the catalytic domain of the enzyme. Propeptide is essential for the enzyme activity. While the structure of the catalytic domain was desidned by homology modeling, the structure of the propeptide has not been resolved yet. In this study, the position where the propeptide is associate

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Krunclová, Tereza. "Výzkum Struktury β-N-Acetylhexosaminidasy z Penicillium oxalicum". Master's thesis, 2012. http://www.nusl.cz/ntk/nusl-308282.

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in English β-N-Acetylhexosaminidase (EC 3.2.1.52) is exoglycosidase, which exhibits the unique properties in the filamentous fungi. Enzyme from these organisms are dimeric, inducible and secreted extracelluary. It is expresed as preproprotein, consists of a signal sequence, a large propeptid and a catalytic subunit. Although the enzyme is widely distributed, its structure differs in varies organisms. Bacteria have only monomeric hexosaminidase. Human enzymes are dimeric as well as fungal, but only hexosaminidase from filamentous fungi have the catalytic subunit noncovalently associated with th

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Xu, Xiaobin. "Mass spectrometry approaches for profiling protein-protein interactions." Thesis, 2014. https://hdl.handle.net/2144/14097.

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This dissertation is focused on developing cross-linking and mass spectrometry methodologies to study protein-protein interactions. Top-down cross-linking, in combination with mass spectrometry, provides advantages over bottom-up approaches, such as retaining posttranslational modification. Intermolecular cross-linking studies focus on defining protein complex topology and protein-protein interactions. We first developed the top-down MS approach to analyze intermolecular cross-linking in human hemoglobin. Both α-α and β-β intermolecular cross-linking were found and the cross-linking sites on t

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Kukačka, Zdeněk. "Charakterizace struktury proteinů pomocí chemického zesítění a hmotnostní spektrometrie." Doctoral thesis, 2015. http://www.nusl.cz/ntk/nusl-334650.

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Some proteins require presence of their specific ligand, cofactor or prosthetic group for their activity. Binding of this specific molecule can cause conformational changes which permit to perform their function. In some occasions the identification of conformational changes could be really challenging task. In this thesis we describe the novel approach for monitoring structural changes in proteins using chemical cross-linking and high resolution mass spectrometry and its application on model calmodulin system. It is demonstrated that analysis using isotope-labelled cross-linking agents enable

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Kadavath, Harindranath. "NMR Spectroscopy of the Tau-Microtubule Interaction." Doctoral thesis, 2014. http://hdl.handle.net/11858/00-1735-0000-0028-866A-0.

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Peach, Retha. "Characterisation of proton exchange membranes in an H₂SO₄ environment / Retha Peach." Thesis, 2014. http://hdl.handle.net/10394/13405.

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In light of the world‟s growing demand for energy that is environmentally friendly and sustainable, energy sources such as hydrogen have been considered potential contenders. Hydrogen, which can be used for energy storage, can be produced efficiently by the membrane based Hybrid Sulfur (HyS) thermo-chemical process consisting of a decomposition and an electrolysis step. During the HyS electrolysis step, SO2 and H2O are converted to H2 and H2SO4, which implies that the proton exchange membranes (PEMs) to be used for this process should have a high proton conductivity, limited SO2 cross-over and

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Ptáčková, Renata. "Aplikační potenciál modifikačních metod (chemická činidla, foto-nanosondy) a hmotnostní spektrometrie pro studium struktury proteinů a jejich vzájemných interakcí." Doctoral thesis, 2015. http://www.nusl.cz/ntk/nusl-336161.

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A comprehensive understanding of physiological role of proteins requires knowledge of their three-dimensional structure, dynamics and protein-protein interactions. Chemical cross-linking in combination with mass spectrometry represents an alternative approach to standard methods for protein structure elucidation (X-ray crystalography, NMR spectroscopy) and enables characterization of interaction interface within protein complexes in their native states. The presented thesis is mainly focused on novel cross-linking methodology based on the in vivo incorporation of methionine analog with photo-r

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Javed, Asif. "Effects of plasticizing and crosslinking on the mechanical and barrier properties of coatings based on blends of starch and poly(vinyl alcohol)." Licentiate thesis, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-38337.

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In the last decades, intensive research has been carried out in order to replace oil-based polymers with bio-based polymers due to growing environmental concerns. So far, most of the barrier materials used in food packaging are petroleum-based materials. The purpose of the barrier is to protect the packaged food from oxygen, water vapour, water and fat. The mechanical and barrier properties of coatings based on starch-plasticizer and starch-poly(vinyl alcohol) (PVOH)-plasticizer blends have been studied in the work described in this thesis. The plasticizers used were glycerol, polyethylene gly

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Růžičková, Barbora. "Studium struktury receptoru DCL-1 pomocí hmotnostní spektrometrie." Master's thesis, 2013. http://www.nusl.cz/ntk/nusl-324111.

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DCL-1 (CD302) is a type I transmembrane C-type lectin receptor, which is expressed on monocytes, macrophages, granulocytes and dendritic cells. However, its extracellular domain lacks the amino acids motives essential for carbohydrate binding in the presence of calcium ions, suggesting that it does not have the classic binding capacity found in other C-type lectin receptors such as the mannose receptor. No exogenous or endogenous ligands have been identified yet, though. Due to internal colocalization with F-actin we can assume, that this unconventional lectin receptor plays a role not only in

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Slavata, Lukáš. "Studium interakce mezi DNA a transkripčními faktory pomocí hmotnostní spektrometrie." Master's thesis, 2015. http://www.nusl.cz/ntk/nusl-332586.

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Transcription factors play crucial regulatory role within the cell and the entire multicellular organism. The important factor is its ability to interact with other regulatory proteins and DNA. Despite the fact that a large part of the interaction network is already documented, detailed information on the structure and dynamics of protein-protein and protein-DNA complexes is still scarce. In this thesis we focused on the possibility of studying conformational changes given by the transcription factor-DNA complex formation using the methods of structural mass spectrometry: hydrogen/deuterium ex

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Hanč, Pavel. "Výzkum vzájemné interakce membránových receptorů NKR-P1D a Clrb." Master's thesis, 2011. http://www.nusl.cz/ntk/nusl-297140.

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Studies on interactions between NKR-P1D and Clrb membrane receptors Interaction between murine NKR-P1D and Clrb receptors was originally described as a novel type of "MHC class-I independent missing-self recognition" and was shown to confer protection from killing by natural killer cells.[1] However, further study brought conflicting results suggesting that NKR-P1D does not binds Clrb strongly if it does at all.[2] In order to address the issues arising from these conflicting results, we have recombinantly expressed the extracellular domains of both receptors in E. coli cells and refolded the

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Rosůlek, Michal. "Studium vlivu kofaktoru na strukturu proteinu pomocí hmotnostní spektrometrie." Master's thesis, 2015. http://www.nusl.cz/ntk/nusl-331768.

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Bacterial protein WrbA from E. coli is the founding member of a new family of FMN-dependent NAD(P)H oxidoreductases, forming a functional and structural bridge between bacterial flavodoxin and certain mammalian NAD(P)H:quinone oxidoreductase. For these reasons, protein WrbA is recently intensively studied using various analytical and computing methods. Protein WrbA participates in the protection of cells against oxidative stress, but precise function of the protein WrbA in vivo is still unknown. Protein WrbA forms multimers in solutions. In μM concentrations and at low temperature (4 řC) the p

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Hernychová, Lucie. "Studium interakce lektinových receptorů přirozených zabíječů s jejich proteinovými ligandy." Master's thesis, 2014. http://www.nusl.cz/ntk/nusl-332175.

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NK cells are innate lymphocytes which constitute the first line of organism's defence against infections through their receptor system. These cells represent an important part of antiviral and antitumor immunity, they also play a role in transplant immunity, autoimmunity and reproduction. This diploma thesis inquires into the structure of the transmembrane receptor NKR-P1B of mouse NK cells and the interaction with its ligand Clr-b. The aim was to prepare the expression vector coding the ligand-binding and whole extracellular region of the receptor NKR-P1B and to optimize its production and re

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