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1

Dias-Alves, Andrea, Oscar Cabezón, Nicole Borel, et al. "Molecular Detection and Identification of Chlamydiaceae in the Eyes of Wild and Domestic Ruminant Hosts from Northern Spain." Pathogens 10, no. 3 (2021): 383. http://dx.doi.org/10.3390/pathogens10030383.

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Infections by Chlamydiae are associated with ocular disease in humans and animals. In this study, the presence and diversity of Chlamydia spp. was assessed in diseased and healthy eyes of domestic sheep and wild ruminants that share mountain habitats in northern Spain. The presence of Chlamydia spp. was tested by real-time PCR in 1786 conjunctival swabs collected from both eyes of 893 animals from mountain habitats in northern Spain, and chlamydial species were identified in the positive samples by ArrayTube microarray methods. Chlamydial DNA was detected in 0.6% (CI95% 0.2–1.3) of the Pyrenea
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2

Jelocnik, Martina. "Chlamydiae from Down Under: The Curious Cases of Chlamydial Infections in Australia." Microorganisms 7, no. 12 (2019): 602. http://dx.doi.org/10.3390/microorganisms7120602.

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In Australia, the most researched and perhaps the most successful chlamydial species are the human pathogen Chlamydia trachomatis, animal pathogens Chlamydia pecorum and Chlamydia psittaci. C. trachomatis remains the leading cause of sexually transmitted infections in Australians and trachoma in Australian Indigenous populations. C. pecorum is globally recognised as the infamous koala and widespread livestock pathogen, whilst the avian C. psittaci is emerging as a horse pathogen posing zoonotic risks to humans. Certainly not innocuous, the human infections with Chlamydia pneumoniae seem to be
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3

Giannitti, Federico, Mark Anderson, Myrna Miller, et al. "Chlamydia pecorum." Journal of Veterinary Diagnostic Investigation 28, no. 2 (2016): 184–89. http://dx.doi.org/10.1177/1040638715625729.

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4

Szymańska-Czerwińska, Mońika, Agnieszka Jodełko, Zbigniew Osiński, Kinga Zaręba-Marchewka, and Krzysztof Niemczuk. "Prevalence of chlamydiae in dairy cattle herds and factors contributing to the spread of infections." Journal of Veterinary Research 68, no. 4 (2024): 531–38. https://doi.org/10.2478/jvetres-2024-0071.

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Abstract Introduction Different Chlamydia species affect cattle and contribute to economic losses. One of them, C. pecorum, is a globally endemic livestock pathogen. Despite its endemicity, prevalence data from Poland have so far been limited. The present study aimed to obtain insight into the chlamydiae prevalence in Polish dairy cattle. Material and Methods A screening of chlamydial seroprevalence in dairy cattle was initially performed, followed by Chlamydiaceae- and species-specific real-time qPCR. Vaginal swabs (n = 239) and placenta samples (n = 2) from seropositive animals in 142 herds
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5

Ксьонз, І. М., І. М. Цівенко, К. Ф. Почерняєв та С. М. Корінний. "РОЗРОБЛЕННЯ ПЛР-ТЕСТ-СИСТЕМИ ДЛЯ ІНДИКАЦІЇ БАКТЕРІЙ РОДУ СHLAMYDIA У БІОЛОГІЧНИХ ЗРАЗКАХ ВІД СВІЙСЬКИХ СОБАК". Вісник Полтавської державної аграрної академії, № 2 (26 червня 2014): 102–4. http://dx.doi.org/10.31210/visnyk2014.02.19.

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Розроблено ПЛР-тест-систему для діагностикихламідіозу свійських собак, до складу якої входятьолігонуклеотидні праймери, що фланкують ділянкугена, який кодує ендорибонуклеазу P (RNase P RNA)Chlamydia abortus, Chlamydia pecorum, Chlamydiapsittaci. Ідентичність продукту ампліфікації підтвер-джено шляхом рестрикційного аналізу з використан-ням ендонуклеази Alu I. Означена ПЛР-тест-системавипробувана на 50-и позитивних і 25-и негативнихзразках ДНК бактерій роду Сhlamydia, виділених зізолятів хламідій від собак, а також успішно пройш-ла валідацію із іншими ПЛР-тест-системами дляіндикації та видової
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6

Baghian, Abolghasem, Konstantin Kousoulas, Robert Truax, and Johannes Storz. "Specific antigens of Chlamydia pecorum and their homologues in C psittaci and C trachomatis." American Journal of Veterinary Research 57, no. 12 (1996): 1720–25. http://dx.doi.org/10.2460/ajvr.1996.57.12.1720.

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Abstract Objective To analyze the antigenic cross reactivity of various proteins of strains of Chlamydia pecorum, C psittaci, and C trachomatis. Samples and Procedures Strains FC-Stra and LW-613 of C pecorum; strains B577, Fitz-9, and 6BC of C psittaci, and strain LGV-2 of C trachomatis were studied. Strains of C pecorum were propagated in Georgia bovine kidney cells, and other chlamydial strains were propagated in L cells or Georgia bovine kidney cells. Partially purified chlamydial elementary bodies propagated in RAG cells, a BALB/c cell line cloned from a renal adenocarcinoma of BALB/c mice
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7

Korniyenko, M. V., та І. М. Кsyonz. "Визначення поліморфних варіантів фрагментів ДНК збудників хламідійних інфекцій сільськогосподарських тварин". Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 19, № 73 (2017): 66–70. http://dx.doi.org/10.15421/nvlvet7314.

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Under the currently existing classification, adopted at the Second European Symposium «Animal Chlamydioses and Zoonotic Implications (EMAC-2)», Chlamydia pathogens of animals and humans are intracellular gram-negative bacteria belonging to Chlamydiales order, Chlamydiaseae family, Chlamydia genus. The above mentioned genus includes 11 species: C. abortus, C. avium, C. caviae, C. felis, C. gallinacea, S. muridarum, C. pecorum, C. pneumoniae, C. psittaci, C. suis and C. trachomatis, 10 of them being pathogenic for animals and Chlamydia trachomatis being exclusively human Chlamydiosis agent. Deve
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8

Timms, Peter. "Chlamydial infection and disease in the koala." Microbiology Australia 26, no. 2 (2005): 65. http://dx.doi.org/10.1071/ma05065.

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Chlamydiae are obligate intracellular bacterial pathogens able to infect and cause serious disease in humans, birds and a remarkably wide range of warm and cold-blooded animals. The family Chlamydiaciae have traditionally been defined by their unique biphasic developmental cycle, involving the interconversion between an extracellular survival form, the elementary body and an intracellular replicative form, the reticulate body. However, as with many other bacteria, molecular approaches including 16SrRNA sequence are becoming the standard of choice. As a consequence, the chlamydiae are in a taxo
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9

Harvey, Erin, Danielle Madden, Adam Polkinghorne, and Edward Holmes. "Identification of A Novel Picorna-Like Virus, Burpengary Virus, that is Negatively Associated with Chlamydial Disease in the Koala." Viruses 11, no. 3 (2019): 211. http://dx.doi.org/10.3390/v11030211.

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Koalas (Phascolarctos cinereus) are native Australian marsupials whose populations are in decline from a range of threats. Infectious diseases caused by the bacterium Chlamydia pecorum and other pathogens are of particular concern. We analysed 26 poly-A selected RNA-sequencing libraries from a data set designed to study the immune response of koalas to ocular chlamydial infection. Using virus discovery techniques, we identified the coding-complete genome sequence of a novel picorna-like virus, denoted Burpengary virus, that was most common in south-east Queensland. Notably, abundance measureme
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10

Jenkins, Cheryl, Martina Jelocnik, Emily Onizawa, et al. "Chlamydia pecorum Ovine Abortion: Associations between Maternal Infection and Perinatal Mortality." Pathogens 10, no. 11 (2021): 1367. http://dx.doi.org/10.3390/pathogens10111367.

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Chlamydia pecorum is a common gastrointestinal inhabitant of livestock but infections can manifest in a broad array of clinical presentations and in a range of host species. While C. pecorum is a known cause of ovine abortion, clinical cases have only recently been described in detail. Here, the prevalence and sequence types (STs) of C. pecorum in ewes from a property experiencing high levels of perinatal mortality (PNM) in New South Wales (NSW), Australia, were investigated using serological and molecular methods. Ewes that were PNM+ were statistically more likely to test seropositive compare
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11

Loehrer, Samuel, Fabian Hagenbuch, Hanna Marti, Theresa Pesch, Michael Hässig, and Nicole Borel. "Longitudinal study of Chlamydia pecorum in a healthy Swiss cattle population." PLOS ONE 18, no. 12 (2023): e0292509. http://dx.doi.org/10.1371/journal.pone.0292509.

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Chlamydia pecorum is a globally endemic livestock pathogen but prevalence data from Switzerland has so far been limited. The present longitudinal study aimed to get an insight into the C. pecorum prevalence in Swiss cattle and investigated infection dynamics. The study population consisted of a bovine herd (n = 308) located on a farm in the north-eastern part of Switzerland. The herd comprised dairy cows, beef cattle and calves all sampled up to five times over a one-year period. At each sampling timepoint, rectal and conjunctival swabs were collected resulting in 782 samples per sampled area
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12

White, Rhys T., Alistair R. Legione, Alyce Taylor-Brown, et al. "Completing the Genome Sequence of Chlamydia pecorum Strains MC/MarsBar and DBDeUG: New Insights into This Enigmatic Koala (Phascolarctos cinereus) Pathogen." Pathogens 10, no. 12 (2021): 1543. http://dx.doi.org/10.3390/pathogens10121543.

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Chlamydia pecorum, an obligate intracellular pathogen, causes significant morbidity and mortality in livestock and the koala (Phascolarctos cinereus). A variety of C. pecorum gene-centric molecular studies have revealed important observations about infection dynamics and genetic diversity in both koala and livestock hosts. In contrast to a variety of C. pecorum molecular studies, to date, only four complete and 16 draft genomes have been published. Of those, only five draft genomes are from koalas. Here, using whole-genome sequencing and a comparative genomics approach, we describe the first t
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13

Everson, J. S., S. A. Garner, B. Fane, B. L. Liu, P. R. Lambden, and I. N. Clarke. "Biological Properties and Cell Tropism of Chp2, a Bacteriophage of the Obligate Intracellular Bacterium Chlamydophila abortus." Journal of Bacteriology 184, no. 10 (2002): 2748–54. http://dx.doi.org/10.1128/jb.184.10.2748-2754.2002.

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ABSTRACT A number of bacteriophages belonging to the Microviridae have been described infecting chlamydiae. Phylogenetic studies divide the Chlamydiaceae into two distinct genera, Chlamydia and Chlamydophila, containing three and six different species, respectively. In this work we investigated the biological properties and host range of the recently described bacteriophage Chp2 that was originally discovered in Chlamydophila abortus. The obligate intracellular development cycle of chlamydiae has precluded the development of quantitative approaches to assay bacteriophage infectivity. Thus, we
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14

Bezborodova, Natalia, Veronika Kozhukhovskaya, Ol'ga Sokolova, Elena Pechura, and Alisa Romanova. "The role of PCR in the diagnosis of species-specific chlamydia in cattle." Agrarian Bulletin of the 204, no. 01 (2021): 30–35. http://dx.doi.org/10.32417/1997-4868-2021-204-01-30-35.

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Abstract. The aim of the study was to study the practical value of the PCR method for the identification of chlamydia species in cattle. Methods. PCR diagnostics of biological material from cows and calves was carried out. Results. In the course of laboratory studies, it was found that in 17.1 % of cases the biological diversity of the chlamydia species was found in the biological material: the genomes of Chlamydia spp. (7.6 %), Chlamydophila pecorum (7.6 %) and Chlamydophila abortus (1.9 %). Specific DNA regions of the bacterium Chlamydophila pecorum were found in biological material from cal
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15

Jelocnik, Martina, and Adam Polkinghorne. "Chlamydia pecorum: successful pathogen of koalas or Australian livestock?" Microbiology Australia 38, no. 3 (2017): 101. http://dx.doi.org/10.1071/ma17042.

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In Australia, the obligate intracellular bacterium Chlamydia pecorum is best known as the notorious koala pathogen that causes debilitating ocular and urogenital tract disease. While globally published data suggests that this species is essentially ubiquitous in livestock, little is known about the epidemiology of livestock C. pecorum infections here in Australia. My research is focused on investigating the genetic diversity and transmission patterns of C. pecorum, and why it causes disease. Using our newly developed C. pecorum-specific molecular epidemiology typing scheme we provided the firs
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16

Struthers, Jason D., Ailam Lim, Sylvia Ferguson, et al. "Meningoencephalitis, Vasculitis, and Abortions Caused by Chlamydia pecorum in a Herd of Cattle." Veterinary Pathology 58, no. 3 (2021): 549–57. http://dx.doi.org/10.1177/0300985820985288.

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A cow dairy ( n = 2000) in close proximity to a sheep flock had third-trimester abortions and fatalities in cows and calves over a 14-month period. Eighteen of 33 aborted fetuses (55%) had multifocal random suppurative or mononuclear meningoencephalitis with vasculitis. Seventeen of these affected fetuses had intracytoplasmic bacteria in endothelial cells, and 1 fetus with pericarditis had similar bacteria within mesothelial cells or macrophages. Immunohistochemistry for Chlamydia spp. or polymerase chain reaction (PCR) for Chlamydia pecorum or both, performed on brain or pooled tissue, were p
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17

FUKUSHI, Hideto, and Katsuya HIRAI. "Chlamydia pecorum the Fourth Species of Genus Chlamydia." Journal of the Japan Veterinary Medical Association 48, no. 1 (1995): 1–6. http://dx.doi.org/10.12935/jvma1951.48.1.

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18

Peel, Emma, Yuanyuan Cheng, Julianne T. Djordjevic, et al. "Koala cathelicidin PhciCath5 has antimicrobial activity, including against Chlamydia pecorum." PLOS ONE 16, no. 4 (2021): e0249658. http://dx.doi.org/10.1371/journal.pone.0249658.

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Devastating fires in Australia over 2019–20 decimated native fauna and flora, including koalas. The resulting population bottleneck, combined with significant loss of habitat, increases the vulnerability of remaining koala populations to threats which include disease. Chlamydia is one disease which causes significant morbidity and mortality in koalas. The predominant pathogenic species, Chlamydia pecorum, causes severe ocular, urogenital and reproductive tract disease. In marsupials, including the koala, gene expansions of an antimicrobial peptide family known as cathelicidins have enabled pro
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19

Kuroda-Kitagawa, Yuko, Chieko Suzuki-Muramatsu, Tsuyoshi Yamaguchi, Hideto Fukushi, and Katsuya Hirai. "Antigenic analysis of Chlamydia pecorum and mammalian Chlamydia psittaci by use of monoclonal antibodies to the major outer membrane protein and a 56- to 64-kd protein." American Journal of Veterinary Research 54, no. 5 (1993): 709–12. http://dx.doi.org/10.2460/ajvr.1993.54.05.709.

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Summary Monoclonal antibodies were prepared against 40- and 56- to 64-kd antigens of Chlamydia pecorum strain Maeda, which was isolated from a cow with pneumonia. Using the monoclonal antibodies, 5 strains of C pecorum, 25 strains of mammalian and 19 strains of avian C psittaci, 1 strain of C pneumoniae, and 3 strains of C trachomatis were analyzed for immunologic reactivity by use of the indirect immunofluorescent test. Monoclonal antibody analysis revealed immunologic relatedness between C pecorum and mammalian strains of C psittaci, which were completely differentiated from the other avian
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20

Chisu, Valentina, Rosanna Zobba, Giovanna Masala, et al. "First Molecular Detection of Zoonotic Chlamydia Species in Vietnamese Goats." Pathogens 11, no. 8 (2022): 903. http://dx.doi.org/10.3390/pathogens11080903.

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The genus Chlamydia comprises obligate intracellular bacteria that infect a wide variety of hosts, with infection leading to a range of diseases in humans and animals; they thus constitute a major public health threat. Among the members of the Chlamydiaceae family, Chlamydia suis, C. abortus, C. pecorum, and C. psittaci represent the most important pathogenic species infecting a large range of hosts and are a well-established threat to livestock. Information regarding the circulation of Chlamydia species in ruminants from Vietnam is lacking. In this study, DNA extracted from 60 blood samples c
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21

Limón-González, Magdalena, Rigoberto Hernández-Castro, Gabriela Palomares Reséndiz, Enrique Herrera López, and Efrén Díaz Aparicio. "Identification of Chlamydia abortus and Chlamydia pecorum in water buffaloes (Bubalus bubalis) and cows cohabitating the same herd." Buffalo Bulletin 42, no. 4 (2023): 511–16. http://dx.doi.org/10.56825/bufbu.2023.4243989.

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The aim of this study was to determine the presence of Chlamydia spp. in a dual-purpose zebu and water buffalo-mixed herd cohabitating in a ranch located in southern Mexico. The zootechnical purpose of this herd is milk and cheese production. A total of 52 vaginal exudate samples were obtained from clinically healthy water buffaloes, less than one month after parturition, and two vaginal exudate samples were taken from cows that had recently aborted. L929 cells were used for bacterial isolation. Two of these cultures were infected, confirmed by direct immunofluorescence. Total DNA was extracte
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22

Zezekalo, V. K., S. B. Peredera, T. V. Buslik, and K. F. Pochernyaev. "PCR-test system specific identification Parachlamydia acanthamoebae." Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 20, no. 92 (2018): 101–4. http://dx.doi.org/10.32718/nvlvet9220.

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Chlamydia-like bacteria Parachlamydia acanthamoebae belongs to the phylum Chlamydiae, class Chlamydiia, order Chlamydiales, family Parahlamydiaceae, genus Parachlamydia. The following terms were introduced to designate Chlamydiales bacteria that are not members of the Chlamydia genus: Chlamydia-related bacteria – CRB, Chlamydia-like bacteria and Chlamydia-like organisms – CLOs. Parachlamydia acanthamoebae is associated with respiratory diseases in humans and ruminants, as well as diseases of the reproductive organs leading to abortions, or the birth of weak or non-viable offspring. Unfortunate
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23

Palmieri, Chiara, Lyndal Hulse, Sara Pagliarani, et al. "Chlamydia pecorum Infection in the Male Reproductive System of Koalas (Phascolarctos cinereus)." Veterinary Pathology 56, no. 2 (2018): 300–306. http://dx.doi.org/10.1177/0300985818806963.

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Chlamydiosis is the most documented and serious disease of koalas, characterized by ocular, urinary, and reproductive lesions. Since little attention has been paid to the pathological effects of this infection in the male reproductive system, we aimed to determine the incidence and severity of reproductive pathology associated with chlamydial infection in male koalas submitted to koala hospitals in southeast Queensland. The entire reproductive tract from 62 sexually mature male koalas not suitable for rehabilitation was evaluated and 677 tissue samples were collected for histology, immunohisto
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24

Meijer, Adam, Servaas A. Morré, Adriaan J. C. Van Den Brule, Paul H. M. Savelkoul, and Jacobus M. Ossewaarde. "Genomic Relatedness of ChlamydiaIsolates Determined by Amplified Fragment Length Polymorphism Analysis." Journal of Bacteriology 181, no. 15 (1999): 4469–75. http://dx.doi.org/10.1128/jb.181.15.4469-4475.1999.

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ABSTRACT The genomic relatedness of 19 Chlamydia pneumoniaeisolates (17 from respiratory origin and 2 from atherosclerotic origin), 21 Chlamydia trachomatis isolates (all serovars from the human biovar, an isolate from the mouse biovar, and a porcine isolate), 6 Chlamydia psittaci isolates (5 avian isolates and 1 feline isolate), and 1 Chlamydia pecorum isolate was studied by analyzing genomic amplified fragment length polymorphism (AFLP) fingerprints. The AFLP procedure was adapted from a previously developed method for characterization of clinical C. trachomatis isolates. The fingerprints of
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25

Higgins, Damien P., Susan Hemsley, and Paul J. Canfield. "Association of Uterine and Salpingeal Fibrosis with Chlamydial Hsp60 and Hsp10 Antigen-Specific Antibodies in Chlamydia-Infected Koalas." Clinical Diagnostic Laboratory Immunology 12, no. 5 (2005): 632–39. http://dx.doi.org/10.1128/cdli.12.5.632-639.2005.

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ABSTRACT Infection by Chlamydia pneumoniae or Chlamydia pecorum commonly causes chronic, fibrotic disease of the urogenital tracts of female koalas. Studies of humans have associated titers of serum immunoglobulin G (IgG) against chlamydial hsp60 and hsp10 antigens with chronic infection, salpingeal fibrosis, and tubal infertility. To determine whether a similar relationship exists in Chlamydia-infected koalas, samples were collected opportunistically from 34 wild female koalas and examined by gross pathology and histopathology, PCR, and immunohistochemistry for Chlamydia spp. and enzyme-linke
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Fukushi, Hideto, and Katsuya Hirai. "Chlamydia pecorum-The Fourth Species of GenusChlamydia-." Microbiology and Immunology 37, no. 7 (1993): 515–22. http://dx.doi.org/10.1111/j.1348-0421.1993.tb01671.x.

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27

Casteriano, Andrea, Astrid Robin Van Aggelen, Shali Fischer, et al. "Evaluation of a biosecurity survey approach for contamination by Chlamydia pecorum in koala rehabilitation, field capture, and captive settings." PeerJ 11 (August 15, 2023): e15842. http://dx.doi.org/10.7717/peerj.15842.

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Transmission of Chlamydia pecorum between koalas is a potential risk in field capture or rehabilitation settings, where koalas are held in proximity to each other, or equipment is shared between animals. Given the impact of C. pecorum on koala welfare and population viability it is surprising that quarantine and disinfection protocols in a koala rehabilitation facility or capture settings have not previously been evaluated. This study aimed to evaluate an approach, based on the detection of chlamydial DNA and cell viability, to determine the degree of environmental contamination within a koala
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Зезекало, В. К., С. Б. Передера та Н. С. Щербакова. "Оновлення таксономічної класифікації мікроорганізмів порядку Chlamydiales". Вісник Полтавської державної аграрної академії, № 1 (29 березня 2019): 207–15. http://dx.doi.org/10.31210/visnyk2019.01.24.

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Мета статті – надати актуальну на сьогоднішній день таксономічну класифікацію порядку Chlamydiales та детально роз’яснити терміни: «Chlamydia-related bacteria (CRB)» (хламідіє-споріднені бактерії,) «Chlamydia-like organisms (CLO)» (хламідіє-подібні організми), «environmental chlamydiae» (екологічні хламідії, хламідії довкілля).
 Методика дослідження. Використано такі методи дослідження: системний аналіз доступних наукових джерел, історичний метод (для вивчення виникнення, формування та розвитку таксономії хламідійних видів у хронологічній послідовності), емпіричний метод (щодо комплексної
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De Jesús Aldama, Fernando, Roberto Montes de Oca Jiménez, and Jorge Antonio Varela Guerrero. "Diagnóstico, prevención y control de enfermedades causadas por Chlamydia en pequeños rumiantes. Revisión." Revista Mexicana de Ciencias Pecuarias 13, no. 3 (2022): 725–42. http://dx.doi.org/10.22319/rmcp.v13i3.5564.

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Las especies que conforman el género Chlamydia afectan una amplia gama de hospederos animales, causando diversas patologías. Chlamydia abortus (C. abortus), Chlamydia psittaci (C. psittaci) y Chlamydia pecorum (C. pecorum) son las de mayor relevancia clínica en pequeños rumiantes a nivel mundial, ya que han sido relacionadas con problemas reproductivos, oculares y del tracto digestivo respectivamente; dos de estas (C. abortus y C. psittaci), representan un riesgo potencial zoonótico al ser humano. El diagnóstico de infecciones por organismos de este género resulta complicado; ya que, en la may
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Jonker, Annelize, and Anita L. Michel. "Optimization and Application of Real-Time qPCR Assays in Detection and Identification of Chlamydiales in Products of Domestic Ruminant Abortion." Pathogens 12, no. 2 (2023): 290. http://dx.doi.org/10.3390/pathogens12020290.

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Domestic ruminant abortions due to infectious agents represent an important cause of economic losses in the agricultural industry. This study aimed to optimise and apply qPCR assays for detection of Chlamydiales in domestic ruminant abortion cases. Primers and probes for detection of the order Chlamydiales, Chlamydia abortus, Chlamydia pecorum, Parachlamydia acanthamoeba and Waddlia chondrophila were taken from the literature to create one singleplex and two duplex assays and the assays were optimised. Placentitis and pneumonia are pathological lesions associated with Chlamydiales infection. I
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Leonard, Cory Ann, Frederic Dewez, and Nicole Borel. "Penicillin G-Induced Chlamydial Stress Response in a Porcine Strain ofChlamydia pecorum." International Journal of Microbiology 2016 (2016): 1–10. http://dx.doi.org/10.1155/2016/3832917.

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Chlamydia pecorumcauses asymptomatic infection and pathology in ruminants, pigs, and koalas. We characterized the antichlamydial effect of the beta lactam penicillin G onChlamydia pecorumstrain 1710S (porcine abortion isolate). Penicillin-exposed and mock-exposed infected host cells showed equivalent inclusions numbers. Penicillin-exposed inclusions contained aberrant bacterial forms and exhibited reduced infectivity, while mock-exposed inclusions contained normal bacterial forms and exhibited robust infectivity. Infectious bacteria production increased upon discontinuation of penicillin expos
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Clune, Tom, Susan Anstey, Vasilli Kasimov, Caroline Jacobson, and Martina Jelocnik. "Real-Time Fluorometric Isothermal LAMP Assay for Detection of Chlamydia pecorum in Rapidly Processed Ovine Abortion Samples: A Veterinary Practitioner’s Perspective." Pathogens 10, no. 9 (2021): 1157. http://dx.doi.org/10.3390/pathogens10091157.

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Traditional methods of detecting Chlamydia pecorum in tissue samples such as polymerase chain reaction or cell culture are laborious and costly. We evaluated the use of a previously developed C. pecorum LAMP assay using minimally processed ovine samples. Cotyledon (n = 16), foetal liver (n = 22), foetal lung (n = 2), and vaginal (n = 6) swabs, in addition to cotyledon (n = 6) and foetal liver (n = 8) tissue samples, were rapidly processed and used for LAMP testing without DNA extraction. Overall, LAMP test results were highly congruent with the in-house reference qPCR, with 80.43% (37/46; 72.7
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Fukushi, H., and K. Hirai. "Proposal of Chlamydia Pecorum sp. nov. for Chlamydia Strains Derived from Ruminants." International Journal of Systematic Bacteriology 42, no. 2 (1992): 306–8. http://dx.doi.org/10.1099/00207713-42-2-306.

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Quigley, Bonnie L., and Peter Timms. "Helping koalas battle disease – Recent advances in Chlamydia and koala retrovirus (KoRV) disease understanding and treatment in koalas." FEMS Microbiology Reviews 44, no. 5 (2020): 583–605. http://dx.doi.org/10.1093/femsre/fuaa024.

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ABSTRACT The iconic Australian marsupial, the koala (Phascolarctos cinereus), has suffered dramatic population declines as a result of habitat loss and fragmentation, disease, vehicle collision mortality, dog attacks, bushfires and climate change. In 2012, koalas were officially declared vulnerable by the Australian government and listed as a threatened species. In response, research into diseases affecting koalas has expanded rapidly. The two major pathogens affecting koalas are Chlamydia pecorum, leading to chlamydial disease and koala retrovirus (KoRV). In the last eight years, these pathog
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Rahman, K. Shamsur, Erfan U. Chowdhury, Anil Poudel, Anke Ruettger, Konrad Sachse, and Bernhard Kaltenboeck. "Defining Species-Specific Immunodominant B Cell Epitopes for Molecular Serology of Chlamydia Species." Clinical and Vaccine Immunology 22, no. 5 (2015): 539–52. http://dx.doi.org/10.1128/cvi.00102-15.

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ABSTRACTUrgently needed species-specific enzyme-linked immunosorbent assays (ELISAs) for the detection of antibodies againstChlamydiaspp. have been elusive due to high cross-reactivity of chlamydial antigens. To identifyChlamydiaspecies-specific B cell epitopes for such assays, we ranked the potential epitopes of immunodominant chlamydial proteins that are polymorphic among allChlamydiaspecies. High-scoring peptides were synthesized with N-terminal biotin, followed by a serine-glycine-serine-glycine spacer, immobilized onto streptavidin-coated microtiter plates, and tested with mono-specific m
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Myers, G. S. A., S. A. Mathews, M. Eppinger, et al. "Evidence that Human Chlamydia pneumoniae Was Zoonotically Acquired." Journal of Bacteriology 191, no. 23 (2009): 7225–33. http://dx.doi.org/10.1128/jb.00746-09.

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ABSTRACT Zoonotic infections are a growing threat to global health. Chlamydia pneumoniae is a major human pathogen that is widespread in human populations, causing acute respiratory disease, and has been associated with chronic disease. C. pneumoniae was first identified solely in human populations; however, its host range now includes other mammals, marsupials, amphibians, and reptiles. Australian koalas (Phascolarctos cinereus) are widely infected with two species of Chlamydia, C. pecorum and C. pneumoniae. Transmission of C. pneumoniae between animals and humans has not been reported; howev
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Kramer, Gianna, Janine Duffy, and Valentina S. A. Mella. "Preliminary Evidence of Chlamydiosis in Koalas of the Greater Geelong Region, Victoria: A Potential Emerging Threat?" Animals 15, no. 14 (2025): 2048. https://doi.org/10.3390/ani15142048.

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Koalas (Phascolarctos cinereus) face many threats, with one of them being the disease chlamydiosis caused by the bacterium Chlamydia pecorum, a cause for infertility and one of the main causes of population decline. In New South Wales, Queensland, and the Australian Capital Territory, the koala is an endangered species, but in Victoria, koalas are widespread and overabundant in some areas. The koala populations in the You Yangs Regional Park (YYRP) and Brisbane Ranges National Park (BRNP) in Victoria are reported to be in decline, although there have been no studies looking into population den
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Dijkman, R., L. Harkema, E. Dijkstra, and R. Dijkman. "Chlamydia pecorum-Associated Pneumonia in an Aborted Goat Fetus." Journal of Comparative Pathology 191 (February 2022): 44. http://dx.doi.org/10.1016/j.jcpa.2021.11.098.

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Wedrowicz, Faye, Jennifer Mosse, Wendy Wright, and Fiona E. Hogan. "Using non-invasive sampling methods to determine the prevalence and distribution of Chlamydia pecorum and koala retrovirus in a remnant koala population with conservation importance." Wildlife Research 45, no. 4 (2018): 366. http://dx.doi.org/10.1071/wr17184.

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Context Pathogenic infections are an important consideration for the conservation of native species, but obtaining such data from wild populations can be expensive and difficult. Two pathogens have been implicated in the decline of some koala (Phascolarctos cinereus) populations: urogenital infection with Chlamydia pecorum and koala retrovirus subgroup A (KoRV-A). Pathogen data for a wild koala population of conservation importance in South Gippsland, Victoria are essentially absent. Aims This study uses non-invasive sampling of koala scats to provide prevalence and genotype data for C. pecoru
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Bezborodova, N. A., V. V. Kozhukhovskaya, N. S. Balysheva, et al. "Molecular-biological study of causes of persistent chlamydial infection in cattle: application of developed pcr test system for detecting chlamydophila abortus." International Journal of Veterinary Medicine, no. 3 (December 19, 2024): 28–35. https://doi.org/10.52419/issn2072-2419.2024.3.28.

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Chlamydial infections in animals are a serious problem spread across many continents. Despite the fact that 22% of the world's countries have reported cases of chlamydial infection in animals, the actual prevalence of this disease may be much higher due to the difficulty of diagnosing it. To date, there is no commercial PCR kit for the detection of Chlamydophila abortus in the Russian Federation. In this regard, the main goal of our study was to conduct a molecular genetic study of the causative agent of chlamydial infection in cattle, using a primer PCR test system developed and optimized by
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Salti-Montesanto, Viviane, Eleana Tsoli, Periklis Papavassiliou, et al. "Diagnosis of ovine enzootic abortion, using a competitive ELISA based on monoclonal antibodies against variable segments 1 and 2 of the major outer membrane protein of Chlamydia psittaci serotype 1." American Journal of Veterinary Research 58, no. 3 (1997): 228–35. http://dx.doi.org/10.2460/ajvr.1997.58.03.228.

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Abstract Objective To develop and evaluate a competitive ELISA (cELISA) specific for detection of antibodies to abortion strains of Chlamydia psittaci and C pecorum that is based on monoclonal antibodies against the 2 segments. Procedure Monoclonal antibodies were screened for binding to ELISA plates coated with elementary bodies, and were selected on the basis of positive competition with experimentally produced sera against C psittaci and lack of competition with anti-C pecorum sera. The cELISA was evaluated with field sera, and the results were compared with those obtained by complement-fix
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Cheong, Heng Choon, Chalystha Yie Qin Lee, Yi Ying Cheok, Grace Min Yi Tan, Chung Yeng Looi, and Won Fen Wong. "Chlamydiaceae: Diseases in Primary Hosts and Zoonosis." Microorganisms 7, no. 5 (2019): 146. http://dx.doi.org/10.3390/microorganisms7050146.

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Bacteria of the Chlamydiaceae family are a type of Gram-negative microorganism typified by their obligate intracellular lifestyle. The majority of the members in the Chlamydiaceae family are known pathogenic organisms that primarily infect the host mucosal surfaces in both humans and animals. For instance, Chlamydia trachomatis is a well-known etiological agent for ocular and genital sexually transmitted diseases, while C. pneumoniae has been implicated in community-acquired pneumonia in humans. Other chlamydial species such as C. abortus, C. caviae, C. felis, C. muridarum, C. pecorum, and C.
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Yang, Rongchang, Caroline Jacobson, Graham Gardner, Ian Carmichael, Angus J. D. Campbell, and Una Ryan. "Longitudinal prevalence and faecal shedding of Chlamydia pecorum in sheep." Veterinary Journal 201, no. 3 (2014): 322–26. http://dx.doi.org/10.1016/j.tvjl.2014.05.037.

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Mackie, J. T., A. K. Gillett, C. Palmieri, T. Feng, and D. P. Higgins. "Pneumonia due to Chlamydia pecorum in a Koala ( Phascolarctos cinereus )." Journal of Comparative Pathology 155, no. 4 (2016): 356–60. http://dx.doi.org/10.1016/j.jcpa.2016.07.011.

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Watt, B., J. McNally, J. Bourke, et al. "O-082 Chlamydia pecorum causing ewe abortions in NSW, Australia." Animal - science proceedings 14, no. 1 (2023): 116. http://dx.doi.org/10.1016/j.anscip.2023.01.158.

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46

Blyton, Michaela D. J., Michael Pyne, Paul Young, and Keith Chappell. "Koala retrovirus load and non-A subtypes are associated with secondary disease among wild northern koalas." PLOS Pathogens 18, no. 5 (2022): e1010513. http://dx.doi.org/10.1371/journal.ppat.1010513.

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Koala Retrovirus (KoRV) has been associated with neoplasia in the vulnerable koala (Phascolarctos cinereus). However, there are conflicting findings regarding its association with secondary disease. We undertook a large-scale assessment of how the different KoRV subtypes and viral load are associated with Chlamydia pecorum infection and a range of disease pathologies in 151 wild koalas admitted for care to Currumbin Wildlife Hospital, Australia. Viral load (KoRV pol copies per ml of plasma) was the best predictor of more disease pathologies than any other KoRV variable. The predicted probabili
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Aaziz, Rachid, Karine Laroucau, Federica Gobbo, et al. "Occurrence of Chlamydiae in Corvids in Northeast Italy." Animals 12, no. 10 (2022): 1226. http://dx.doi.org/10.3390/ani12101226.

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Chlamydiaceae occurrence has been largely evaluated in wildlife, showing that wild birds are efficient reservoirs for avian chlamydiosis. In this study, DNA extracted from cloacal swabs of 108 corvids from Northeast Italy was screened for Chlamydiaceae by 23S real-time (rt)PCR. The positive samples were characterised by specific rtPCRs for Chlamydia psittaci, Chlamydia abortus, Chlamydia gallinacea, Chlamydia avium, Chlamydia pecorum and Chlamydia suis. Cloacal shedding of Chlamydiaceae was detected in 12 out of 108 (11.1%, 5.9%–18.6% 95% CI) corvids sampled. Molecular characterisation at the
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Zezekalo, V. K. "Chlamydiales order bacteria in swine with reproductive disoders. Detection of Waddlia chondrophila in swine." Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 21, no. 95 (2019): 122–26. http://dx.doi.org/10.32718/nvlvet9523.

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Chlamydial infections cause significant economic expenses due to infertility, insufficient productivity growth, and high mortality rates among young stock. Bacteria of the Chlamydiaceae family (Chlamydia suis Chlamydia abortus, Chlamydia psittaci and Chlamydia pecorum) are well known pathogens isolated from pigs. The diseases associated with these species are widely studied; monitoring and prevention are carried out. However, chlamydia-related bacteria in swine remain poorly studied in Ukraine. The purpose of the study was to check samples, taken from swine from private farms in the Poltava re
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Bachmann, Nathan L., Tamieka A. Fraser, Claire Bertelli, et al. "Comparative genomics of koala, cattle and sheep strains of Chlamydia pecorum." BMC Genomics 15, no. 1 (2014): 667. http://dx.doi.org/10.1186/1471-2164-15-667.

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Mojica, S., H. Huot Creasy, S. Daugherty, et al. "Genome Sequence of the Obligate Intracellular Animal Pathogen Chlamydia pecorum E58." Journal of Bacteriology 193, no. 14 (2011): 3690. http://dx.doi.org/10.1128/jb.00454-11.

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