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Academic literature on the topic 'Chlorophyle a fluorescence'

Author: Grafiati

Published: 4 June 2021

Last updated: 13 February 2022

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Journal articles on the topic "Chlorophyle a fluorescence"

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Kummerová, M., and L. Váňová. "Chlorophyll fluorescence as an indicator of fluoranthene phototoxicity." Plant, Soil and Environment 53, No. 10 (January 7, 2008): 430–36. http://dx.doi.org/10.17221/2197-pse.

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The effect of the short-time exposure (12, 24 and 48 h) of increasing concentration (0.1, 1 and 10 mg/l) of intact (FLT) and photo-modified (phFLT) fluoranthene on the chlorophyll fluorescence parameters (<i>F</i><sub>0</sub>, <i>F</i><sub>V</sub>/<i>F</i><sub>M</sub> and Φ<sub>II</sub>) in pea plants (<i>Pisum sativum</i> L. cv. Lantra) was investigated. Plants took up both forms of fluoranthene by two different ways, via roots or via leaves. The obtained results demonstrated a significant increase in <i>F</i><sub>0</sub> and decrease in <i>F</i><sub>V</sub>/<i>F</i><sub>M</sub> and Φ<sub>II</sub> in plants treated by 1 and 10 mg/l FLT and phFLT. An earlier response to presence of FLT and phFLT in the environment was demonstrated by application on cut leaves. The primary processes of photosynthesis were not significantly influenced by short-time phFLT treatment.

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Brody, S. S. "The Position of Carotene in the D-1/D-2 Sub-Core Complex of Photosystem II." Zeitschrift für Naturforschung C 43, no. 3-4 (April 1, 1988): 226–30. http://dx.doi.org/10.1515/znc-1988-3-413.

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When the sub-core complex of photosystem II, D1/D2, is irradiated at 436 or 415 nm (absorption by chlorophyll and pheophytin and β-carotene) or 540 nm (absorption primarily by pheophytin), the low temperature fluorescence spectrum has two maxima, at 685 and 674 nm. This shows the existence of at least two different fluorescent forms of chlorophyll (chlorophyll a and perhaps pheophytin a). When carotene is irradiated at 485 nm (absorption primarily by β-carotene), only fluorescence at 685 nm is observed: this indicates that carotene is transferring energy to only the long-wavelength form of chlorophyll in the D1/D2 sub-core complex. The band of carotene (at 485 nm) does not appear in the fluorescence excitation spectrum, measured at 674 nm. The position of the carotene molecule relative to each of the fluorescent forms of chlorophyll was determined from the excitation spectra of each of the fluorescence bands.

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Oh, Thomas, Jittiwat Sermsripong, and Barry W. Hicks. "Using Scuba for In Situ Determination of Chlorophyll Distributions in Corals by Underwater Near Infrared Fluorescence Imaging." Journal of Marine Science and Engineering 8, no. 1 (January 18, 2020): 53. http://dx.doi.org/10.3390/jmse8010053.

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Studies reporting quantitation and imaging of chlorophyll in corals using visible fluorescent emission in the red near 680 nm can suffer from competing emission from other red-emitting pigments. Here, we report a novel method of selectively imaging chlorophyll distributions in coral in situ using only the near infrared (NIR) fluorescence emission from chlorophyll. Commercially available equipment was assembled that allowed the sequential imaging of visible, visible-fluorescent, and NIR-fluorescent pigments on the same corals. The relative distributions of chlorophyll and fluorescent proteins (GFPs) were examined in numerous corals in the Caribbean Sea, the Egyptian Red Sea, the Indonesian Dampier Strait, and the Florida Keys. Below 2 m depth, solar induced NIR chlorophyll fluorescence can be imaged in daylight without external lighting, thus, it is much easier to do than visible fluorescence imaging done at night. The distributions of chlorophyll and GFPs are unique in every species examined, and while there are some tissues where both fluorophores are co-resident, often tissues are selectively enriched in only one of these fluorescent pigments. Although laboratory studies have clearly shown that GFPs can be photo-protective, their inability to prevent large scale bleaching events in situ may be due to their limited tissue distribution.

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Jay, Aicha El, Jean-Marc Ducruet, Jean-Claude Duval, and Jean Pierre Pelletier. "A high-sensitivity chlorophyll fluorescence assay for monitoring herbicide inhibition of photosystem II in the chlorophyte Selenastrum capricornutum: Comparison with effect on cell growth." Fundamental and Applied Limnology 140, no. 2 (September 25, 1997): 273–86. http://dx.doi.org/10.1127/archiv-hydrobiol/140/1997/273.

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Alexander, Troy A., Guan-Hong Gao, and Chieu D. Tran. "Development of a Novel Fluorimeter Based on Superluminescent Light-Emitting Diodes and Acousto-Optic Tunable Filter and its Application in the Determination of Chlorophylls a and b." Applied Spectroscopy 51, no. 11 (November 1997): 1603–6. http://dx.doi.org/10.1366/0003702971939578.

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A novel, compact, inexpensive fluorimeter that has high sensitivity and no moving parts has been developed by using super luminescent (bright-blue) light-emitting diodes (SLEDs) and an acousto-optic tunable filter (AOTF). In this instrument, the recently developed gallium nitride SLEDs were used in a counterpropagating configuration to provide excitation light. These SLEDs provide not only high intensity (several milliwatts) but also wide spectral bandwidth in the blue region (from 370 to 570 nm). The AOTF can be placed before the sample to facilitate the measurements of excitation spectra or after the sample for the emission spectra measurements. This fluorimeter is suitable for the sensitive and general fluorescent analysis of a variety of compounds. It has been used, as an example, for the sensitive and simultaneous determination of chlorophylls a and b. Detection limits of 2.30 × 10−9 and 1.10 × 10−9 M have been achieved for chlorophyll a and chlorophyll b, respectively. Index Headings: Superluminescent light-emitting diode; Acousto-optic tunable filter; Fluorescence; Chlorophyll.

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Chen, Min, Martin Schliep, Robert D. Willows, Zheng-Li Cai, Brett A. Neilan, and Hugo Scheer. "A Red-Shifted Chlorophyll: Fig. 1." Science 329, no. 5997 (August 19, 2010): 1318–19. http://dx.doi.org/10.1126/science.1191127.

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Chlorophylls are essential for light-harvesting and energy transduction in photosynthesis. Four chemically distinct varieties have been known for the past 60 years. Here we report isolation of a fifth, which we designate chlorophyll f. Its in vitro absorption (706 nanometers) and fluorescence (722 nanometers) maxima are red-shifted compared to all other chlorophylls from oxygenic phototrophs. On the basis of the optical, mass, and nuclear magnetic resonance spectra, we propose that chlorophyll f is [2-formyl]-chlorophyll a (C55H70O6N4Mg). This finding suggests that oxygenic photosynthesis can be extended further into the infrared region and may open associated bioenergy applications.

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Siebke, Katharina, and Marilyn C. Ball. "Non-destructive measurement of chlorophyll b:a ratios and identification of photosynthetic pathways in grasses by reflectance spectroscopy." Functional Plant Biology 36, no. 11 (2009): 857. http://dx.doi.org/10.1071/fp09201.

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Equations for non-destructive determination of chlorophyll b : a ratios in grasses were developed from reflectance spectra of intact leaves of barley (Hordeum vulgare L.) and two barley mutants: clorina f2, which lacks chlorophyll b and clorina f104, which has a low chlorophyll b content. These plants enabled separation of effects of chlorophyll composition on reflectance spectra due to differential light absorption by chlorophylls a and b and to measure the effects of chlorophyll b on the contribution of fluorescence emitted by chlorophyll a to the reflectance spectra. Indices developed from these data were then tested on growth chamber-grown leaves from six C3 and 17 C4 grass species (7 NAD-ME and 10 NADP-ME subtypes). We used the chlorophyll b : a ratio because the data were less skewed than the chlorophyll a : b ratio. The best index for determination of the chlorophyll b : a ratio utilised wavelengths affected by chlorophyll absorbance: [R626 – 0.5 (R603 + R647)]/[R552– R626]. The chlorophyll b : a ratio was significantly lower in the C4 than C3 grasses, but was not sufficient in itself to separate these two functional groups. However, because of differences in fluorescence characteristics, C3 and C4 species could be distinguished by an index based on wavelengths affected by chlorophyll fluorescence: [R696 to 709/R545 to 567].

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De Mesquita Alves, Jackson, Alex Serafim De Lima, Francisco Romário Andrade Figueiredo, Toshik Iarley Da Silva, Lourival Ferreira Cavalcante, Francisco De Oliveira Mesquita, Evandro Franklin De Mesquita, and Cesenildo De Figueiredo Suassuna. "Chlorophyll a fluorescence and development of zucchini plants under nitrogen and silicon fertilization." Agronomía Colombiana 38, no. 1 (January 1, 2020): 45–52. http://dx.doi.org/10.15446/agron.colomb.v38n1.79172.

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Zucchini (Cucurbita pepo L.) has a great economic and productive potential in the semi-arid region of Brazil, due to the wide acceptance by consumers and quick financial return. The nitrate (NO3-) assimilation process in C3 plants such as zucchini is related to photorespiration, and Si accumulated near the stomata reduces the transpiration rate, making the photosynthesis cycle more efficient. The objective of this study was to evaluate the interaction between nitrogen and silicon fertilization ongrowth, chlorophyll index, and chlorophyll a fluorescence of zucchini plants. The treatments were distributed in a split-plot scheme in a randomized block design with three replicates. The plot was arranged by silicon levels (0 and 6 g/plant) and the subplots constituted by five nitrogen levels (30, 60, 90, 120 and 150 kg ha-1). Leaf, stem and total dry masses, chlorophylla, chlorophyll b, total chlorophyll, chlorophyll a/b ratio and chlorophyll a fluorescence were evaluated. The highest dry matter productions in zucchini were obtained in treatments without Si. Si and N application together positively influences the chlorophyll a/b ratio of zucchini plants. The interaction between Si and N positively influences the maximum fluorescence, variable fluorescence and quantum yield of photosystemII of zucchini plants.

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Gruszecki, Wiesław I., Konka Veeranjaneyulu, and Roger M. Leblanc. "Qualitative changes in the fluorescence spectra of intact pea leaves after photoinhibition." Biochemistry and Cell Biology 69, no. 5-6 (May 1, 1991): 399–403. http://dx.doi.org/10.1139/o91-060.

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In high light (1400 W m−2) treated, intact pea leaves, a decrease in the ratio of fluorescence emission at 685 to 730 nm and an increase in fluorescence intensity between 500 and 600 nm were observed. Furthermore, photoacoustically monitored heat emission increased slightly, and O2 evolution decreased significantly. These findings are interpreted as effects of a photoprotective mechanism separating the carotenoid pool from the chlorophylls. This is supported by fluorescence excitation measurements and the results of a study on the reversibility of the process.Key words: chlorophyll fluorescence, carotenoid fluorescence, photoinhibition, photosynthesis, energy transfer.

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Khanizadeh, Shahrokh, and Jennifer Deell. "Chlorophyll Fluorescence." Small Fruits Review 1, no. 3 (July 6, 2001): 61–67. http://dx.doi.org/10.1300/j301v01n03_06.

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Dissertations / Theses on the topic "Chlorophyle a fluorescence"

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Hodges, M. "Chlorophyll fluorescence and thylakoid membrane organisation." Thesis, Imperial College London, 1985. http://hdl.handle.net/10044/1/37728.

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Pniewski, Filip Franciszek. "Capacité photosynthétique du microphytobenthos des vasières intertidales de la Baie de l'Aiguillon (Côte atlantique, France) et des lagunes non-tidales de faible profondeur de la Baie de Puck (Mer Baltique, Pologne)." Thesis, La Rochelle, 2010. http://www.theses.fr/2010LAROS300.

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Dans les écosystèmes littoraux, les communautés microphytobenthiques sont soumises à des conditions environnementales qui peuvent être extrêmes en particulier en ce qui concerne l'intensité lumineuse. Les mécanismes de protection mis en place dépendent étroitement du type d'habitat où se développent ces communautés et cette thèse a pour objectifs d'analyser les caractéristiques de l'activité photosynthétique et les mécanismes de protection développés par des assemblages microphytobenthiques dans deux écosystèmes littoraux très différents : les vasières intertidales atlantiques de la Baie de L'Aiguillon (France) et la lagune côtière non tidale de Puck Bay dans la Mer Baltique (Władysławowo, Pologne). Pour réaliser ces objectifs, trois études ont été réalisées : (1) la description des communautés microphytobenthiques, (2) la caractérisation de leur activité photosynthétique et (3) l'analyse des mécanismes de photoinhibition et de photoprotection.La structure taxonomique du microphytobenthos a été décrite en se basant sur des observations en microscopie optique et sur la mesure des caractéristiques des pigments photosynthétiques par chromatographie liquide à haute performance (HPLC). L'activité photosynthétique a été étudiée par des méthodes de microrespirométrie volumétrique et de spectrofluorométrie de la chlorophylle a. Les mécanismes de photoinhibition et de photoprotection ont été étudiés par fluorométrie en modulation d'amplitude pulsée (PAM).Les résultats obtenus nous ont permis de montrer que :1) Les communautés atlantiques sont fortement dominées par des diatomées épipéliques, alors que le microphytobenthos de la Mer Baltique est plus diversifié et comporte, outre des diatomées, une large part de cyanobactéries,2) Les microphytobenthos atlantique est bien acclimaté à des valeurs d'intensités lumineuses plutôt faibles, alors que les communautés de la Mer Baltique ont encore une bonne activité photosynthétique à de fortes irradiances,3) Les diatomées atlantiques présentent une plus forte photoinhibition que les microalgues de la Baltique,4) L'activité photosynthétique des communautés microphytobenthiques non perturbées montre un des rythmes circadien et tidal, qui semblent être contrôlés par des facteurs endogènes, qui mettent en jeu des adaptations comportementales comme la migration verticale pour les diatomées atlantiques,5) En ce qui concerne le microphytobenthos de la Mer Baltique, qui n'a pas de capacité migratoire, la photoprotection est assurée en premier lieu par la mise en jeu de processus physiologiques. Nous avons pu montrer la très grande flexibilité du photsystème PSII qui est capable de suivre très rapidement les changements à court terme de lumière ambiante
The scope of this thesis includes the characteristics and comparison of photosynthetic activity and photoprotection mechanisms of microphytobenthos assemblages inhabiting the Atlantic intertidal mudflats of Aiguillon Bay (Esnandes, France) and the littoral zone of the Baltic Sea in non-tidal Puck Bay (Władysławowo, Poland). In order to accomplish the main aims of the work the following tasks were carried out: (1) characterization of microphytobenthic assemblages; (2) characterization of their photosynthetic activity and (3) description of photoinhibition and photoprotective mechanisms. The structure of microphytobenthos was described based on observation of the material in light microscope (LM) and through the characteristics of photosynthetic pigments using high performance liquid chromatography (HPLC). Photosynthetic activity was described using various methods including classical (volumetric micro-respirometer) and modern (chlorophyll a fluorescence) ones. In addition, the measurements of variable fluorescence were also used to study photoinhibition and photoprotective mechanisms. Based on the obtained results it was stated that:1.) the Atlantic assemblages were strongly dominated by epipelic diatoms, while the Baltic microphytobenthos was more diverse and cyanobacteria, next to diatoms, were also very important component,2.) it was shown that the Atlantic microphytobenthos was well acclimated to rather low light intensities, while the Baltic assemblages showed good utilization of higher irradiance,3.) the Atlantic diatoms were more susceptible to photoinhibition than the Baltic microalgae,4.) the photosynthetic activity described for the undisturbed microphytobenthos communities revealed circadian and circatidal rhythms, which seemed to be controlled by endogenous factors, supporting diatoms’ behavioural adaptations i.e., vertical migration,5.) in case of the Baltic microphytobenthos, the lack of the ability to move caused their physiological processes the first line of defence against excess irradiances. The analysis revealed extreme flexibility of PSII which was able to follow rapidly the short-term changes in ambient light

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Force, Lesleigh Eileen. "Applications of the JIP-test of chlorophyll fluorescence /." [St. Lucia, Qld.], 2001. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe16307.pdf.

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Cendrero, Mateo Maria del Pilar. "Chlorophyll Fluorescence Response to Water and Nitrogen Deficit." Diss., The University of Arizona, 2013. http://hdl.handle.net/10150/312504.

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The increasing food demand as well as the need to predict the impact of warming climate on vegetation makes it critical to find the best tools to assess crop production and carbon dioxide (CO₂) exchange between the land and atmosphere. Photosynthesis is a good indicator of crop production and CO₂ exchange. Chlorophyll fluorescence (ChF) is directly related to photosynthesis. ChF can be measured at leaf-scale using active techniques and at field-scales using passive techniques. The measurement principles of both techniques are different. In this study, three overarching questions about ChF were addressed: Q1) How water, nutrient and ambient light conditions determine the relationships between photosynthesis and ChF? Which is the optimum irradiance level for detecting water and nutrient deficit conditions with ChF?; Q2) which are the limits within which active and passive techniques are comparable?; and Q3) What is the seasonal relationship between photosynthesis and ChF when nitrogen is the limiting factor? To address these questions, two main experiments were conducted: Exp1) Concurrent photosynthesis and ChF light-response curves were measured in camelina and wheat plants growing under (i) intermediate-light and (ii) high-light conditions respectively. Plant stress was induced by (i) withdrawing water, and (ii) applying different nitrogen levels; and Exp2) coincident active and passive ChF measurements were made in a wheat field under different nitrogen treatments. The results indicated ChF has a direct relationship with photosynthesis when water or nitrogen drives the relationship. This study demonstrates that the light level at which plants were grown was optimum for detecting water and nutrient deficit with ChF. Also, the results showed that for leaf-average-values, active measurements can be used to better understand the daily and seasonal behavior of passive ChF. Further, the seasonal relation between photosynthesis and ChF with nitrogen stress was not a simple linear function. Our study showed that at times in the season when nitrogen was sufficient and photosynthesis was highest, ChF decreased because these two processes compete for available energy. These results demonstrated that ChF is a reliable indicator of crop stress and has great potential for better understand the CO₂ exchange between the land and atmosphere.

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MATOS, ANA GABRIELA BARBOSA. "CHLOROPHYLL A DETERMINATION IN MARINE WATER BY FLUORESCENCE." PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO DE JANEIRO, 2001. http://www.maxwell.vrac.puc-rio.br/Busca_etds.php?strSecao=resultado&nrSeq=2198@1.

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COORDENAÇÃO DE APERFEIÇOAMENTO DO PESSOAL DE ENSINO SUPERIOR
A clorofila a é um composto-chave no processo de absorção e aproveitamento da energia luminosa na fotossíntese. Monitorar a fluorescência da clorofila a para obter informações do aparato fotossintético de produção de energia é uma abordagem atraente porque a fluorescência á percebida externamente às células, podendo ser detectada sem destruir sua fonte. Estudos anteriores realizados pelo Laboratório de Hidrobiologia (UFRJ) e pelo Laboratório de Monitoramento Ambiental Remoto (LabMAR) (PUC-Rio) em águas marinhas indicaram a existência de uma relação linear entre os valores absolutos obtidos pelo Laboratório de Hidrobiologia para a concentração da clorofila a e os valores relativos obtidos pelo LabMAR para a sua fluorescência. Este resultado motivou os dois laboratórios a obter valores absolutos para a concentração da clorofila a em águas marinhas, a partir da medida de sua fluorescência, com a maior confiabilidade possível para, então, relacioná-los aos valores relativos fornecidos pelo LIDAR-PUC. Neste sentido, a implantação de um programa de controle de qualidade no Laboratório de Hidrobiologia indicou que este laboratório encontra-se em condições de obter valores confiáveis para a concentração da clorofila a em amostras de águas marinhas através da fluorimetria. No entanto, uma avaliação rigorosa da relação entre a intensidade da fluorescência da clorofila a (normalizada pela intensidade da emissão do espalhamento Raman da água) e o respectivo valor confiável para a concentração da clorofila a se faz necessária.
Chlorophyll a is a key-compound in the process of light absorption in the photosynthesis. Monitor the chlorophyll a fluorescence to obtain information about the photosynthetic apparatus of energy production is attractive because the chlorophyll a fluorescence could be detected without destruction of the source. Studies performed by the Laboratório de Hidrobiologia (UFRJ) and by the Laboratório de Monitoramento Ambiental Remoto (LabMAR) (PUC-Rio) in marine water samples indicated a linear relation between the absolute values obtained by the former for the chlorophyll a concentration and the relative values obtained by the latter for the chlorophyll a fluorescence. This result motivated both laboratories to obtain absolute values for the chlorophyll a concentration, in marine water samples, as confident as possible and, then, relate these values to the relative values generated by the LIDAR-PUC. In this way, the introduction of a quality control program in the Laboratório de Hidrobiologia indicated that this laboratory is able to analyse marine water samples and to obtain confident values for the chlorophyll a concentration by fluorimetry. However, a more rigorous evaluation of the relation between the chlorophyll a fluorescence and the respective confident value for the chlorophyll a concentration is still necessary.

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Wentworth, Mark. "Quenching of chlorophyll fluorescence in plant light-harvesting complexes." Thesis, University of Sheffield, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.340168.

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Ryan-Keogh, Thomas J. "Understanding the role of chlorophyll fluorescence in nutrient stress." Thesis, University of Southampton, 2014. https://eprints.soton.ac.uk/362003/.

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Phytoplankton exert a dominant influence on the biogeochemical cycling of the oceans, but iron limitation in this dynamic environment can exert a control on photosynthesis. Phytoplankton evolved coping mechanisms to overcome and alleviate the effects of iron limitation. One mechanism is the alteration of the thylakoid membrane and the expression of chlorophyll-binding proteins, which can alter the variable chlorophyll fluorescence signal. Firstly, a study of the chlorophyll-binding iron-stress induced protein, IsiA, in Synechocystis PCC 6803 revealed a 60% increase under iron limitation, in agreement with the theoretical increase. On progressive iron-stress IsiA continued to accumulate without a concomitant increase in _PSI, while Fv/Fm, a measure of photochemical efficiency, continued to decrease. Secondly an oceanographic study to the high latitude North Atlantic in which chlorophyll fluorescence kinetics were used to measure the response to iron addition of in situ phytoplankton populations. The difference in the Fv/Fm between nutrient amended and control treatments (_(Fv/Fm)) was used as a measure of the relative degree of iron stress. The combined observations of both longterm (> 24 h) and short-term (24 h) indicated variability in the seasonal cycle of iron stress, with phytoplankton iron stress developing during the transition from prebloom to peak bloom conditions. Thirdly, similar physiological characteristics were also observed in an oceanographic study in the Ross Sea. The results further confirmed the highly variable response across temporal and spatial scales, but also within different phytoplankton groups. Consistent across all three studies is the reduction in Fv/Fm as the result of an elevated Fo signal, representing potentially unbound chlorophyll-binding proteins. These unbound chorophyll-binding proteins can dominate the total cellular chlorophyll, at least in culture, and reflect a large resource investment. These proteins may provide a rapid source of chlorophyll upon iron resupply. Irrespective of the underlying causes of unbound chlorophyll-binding proteins, the potential large scale expression of such complexes provides a powerful diagnostic tool with which to investigate iron stress in situ.

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Toomey, Heidi M. "Chlorophyll Fluorescence and Thermal Stress in Archaias angulatus (Class Foraminifera)." Thesis, University of South Florida, 2014. http://pqdtopen.proquest.com/#viewpdf?dispub=1549223.

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Benthic foraminifers that host algal symbionts are similar to corals in that they rely on their algal endosymbionts for their energy needs, calcify prolifically, and are sensitive to changes in environmental conditions. They are abundant in the benthos of coastal coral-reef areas and are found throughout the tropical and subtropical regions. Pulse Amplitude Modulated (PAM) chlorophyll fluorometry and chlorophyll a extraction techniques were used to quantify and compare the photosynthetic responses of the benthic foraminifera, Archaias angulatus and their isolated endosymbionts, Chlamydomonas hedleyi, to short-term changes in temperature. Maximum quantum efficiency (Fv/Fm) and rapid light curves (RLCs), from which relative electron transport rates (rETR) of photosystem II (PSII) were derived, were investigated over a thermal range from 4.4° to 33.9 °C in three experiments that were 7 to 31 days in duration. Typical mean yields (Fv/Fm) for healthy holobionts (symbionts in hospite) were 0.6 - 0.7, and for isolated symbionts 0.5 - 0.6. Chronic photoinhibition, indicated by significant decreases in Fv/Fm, occurred at temperatures above 31.0°C; there was minimal reduction in efficiency in cooler treatments. The trends between holobiont and symbionts were very similar in all of the photophysiological parameters measured [yield, photoefficiency (á), ETRmax and minimum saturating irradiance (Ek)] and supported the temperature range findings in terms of the tolerance of the specimens in the low temperatures up to 31.0 °C. For all photochemical measurements assessed, the holobiont values tended to be somewhat higher than those for the symbionts, with the exception of Ek, possibly indicating a tight coupling in the host-symbiont response during photosynthesis. Chlorophyll a (ìg/foram) was negatively correlated with temperature (r = -0.37, p < 0.001) in Experiments 1 and 2. However, in all 3 experiments, chlorophyll a was variable, suggesting a high degree of individual variability in A. angulatus and the ability to acclimate. Some differences observed among treatments may be related to differences in seasons when the specimens were collected and in length of time in culture prior to experiments. Holobiont median rETR light curve trends and photophysiological derived parameters recorded median Ek ranges of ~100-150 ìmol photons m^-2 s^-1, observed ETR_max light intensities of ~200 ìmol photons m-2 s-1 and photoinhibition, induced by increasing irradiance intensities, which occurred > 500 ìmol photons m^-2 s^-1. These light curve trends and derived parameters generally supported previous photosynthesis O₂ and CO₂ gas production studies of A. angulatus. The differences in responses associated with acclimation should be considered in design of future experimental studies. This was the first known physiological study of the viable temperature range and photobiology of A. angulatus using chlorophyll fluorometry methods. Though commonly found in Caribbean and Atlantic waters ranging from 14.0 – 31.0 °C, these results indicate a wider thermal-tolerance range for A. angulatus than was previously known.

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au, jcos@iinet net, and Jeffrey John Cosgrove. "Marine phytoplankton primary production and ecophysiology using chlorophyll-A fluorescence." Murdoch University, 2007. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20071129.122222.

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Marine phytoplankton ecophysiological state and primary production measurements have typically been controversial due to potential impacts of measurement techniques. Advances in chl-a fluorescence techniques have provided a means for rapid, non-invasive measurement of electron transport through photosystem 2 (PSII) in dilute phytoplankton suspensions. While studies on higher plants have outlined a close relationship between PSII electron transport and carbon fixation, results from studies on microalgae reveal significant variations in the relationship. Three species of phytoplankton representing three major taxonomic groups of the marine phytoplankton were used in this study: (1) Chaetoceros muelleri CS176 Lemmermann (Bacillariophyta), (2) Isochrysis galbana CS177 Parke (Haptophyta) and, (3) Nannochloropsis oculata CS179 (Droop) Hibberd (Ochrophyta, eustigmatophyte). Each species was cultured in semicontinuous culture and primary production was estimated using oxygen evolution and carbon fixation techniques and compared against predictions based on chl-a fluorescence measurements. It was found that predicted values of primary production both under-estimated and overestimated actual carbon fixation measured via radioisotope (14C) techniques. This variation was primarily explained by probable errors in the assumed values for PSII density. The relationship between oxygen evolution or carbon fixation with chl-a fluorescence-derived measures was commonly linear below the light saturation parameter, with a departure from linearity occurring at higher irradiances. This departure from linearity was greatest in cultures adapted to low light conditions. At higher light intensities alternative electron pathways such as the Mehler reaction and/or chlororespiration are likely to be more active in low light-adapted cultures, leading to this greater non-linearity. Chl-a fluorescence measurements were also found to be a useful in characterising ecophysiology using photosynthesis-versus irradiance curves. However, an important caveat on this is the measurement of PSII density (çPSII) rather than use of an assumed value as changes in çPSII can have a profound impact on light curve parameters. A field study in Fremantle Harbour found a healthy (negligible nutrient starvation), diatom dominated, phytoplankton community. Results suggest that phytoplankton are able to begin boosting photosynthetic capability just prior to morning twilight. Waters in the harbour were well mixed via tidal motion and substantial midday photoinhibition was not observed. Data suggest levels of primary production at the mouth of the harbour are similar to those of coastal waters in the plume of the Ocean Reef wastewater outfall.

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Cosgrove, Jeffrey John. "Marine phytoplankton primary production and ecophysiology using chlorophyll-A fluorescence." Cosgrove, Jeffrey John (2007) Marine phytoplankton primary production and ecophysiology using chlorophyll-A fluorescence. PhD thesis, Murdoch University, 2007. http://researchrepository.murdoch.edu.au/503/.

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Marine phytoplankton ecophysiological state and primary production measurements have typically been controversial due to potential impacts of measurement techniques. Advances in chl-a fluorescence techniques have provided a means for rapid, non-invasive measurement of electron transport through photosystem 2 (PSII) in dilute phytoplankton suspensions. While studies on higher plants have outlined a close relationship between PSII electron transport and carbon fixation, results from studies on microalgae reveal significant variations in the relationship. Three species of phytoplankton representing three major taxonomic groups of the marine phytoplankton were used in this study: (1) Chaetoceros muelleri CS176 Lemmermann (Bacillariophyta), (2) Isochrysis galbana CS177 Parke (Haptophyta) and, (3) Nannochloropsis oculata CS179 (Droop) Hibberd (Ochrophyta, eustigmatophyte). Each species was cultured in semicontinuous culture and primary production was estimated using oxygen evolution and carbon fixation techniques and compared against predictions based on chl-a fluorescence measurements. It was found that predicted values of primary production both under-estimated and overestimated actual carbon fixation measured via radioisotope (14C) techniques. This variation was primarily explained by probable errors in the assumed values for PSII density. The relationship between oxygen evolution or carbon fixation with chl-a fluorescence-derived measures was commonly linear below the light saturation parameter, with a departure from linearity occurring at higher irradiances. This departure from linearity was greatest in cultures adapted to low light conditions. At higher light intensities alternative electron pathways such as the Mehler reaction and/or chlororespiration are likely to be more active in low light-adapted cultures, leading to this greater non-linearity. Chl-a fluorescence measurements were also found to be a useful in characterising ecophysiology using photosynthesis-versus irradiance curves. However, an important caveat on this is the measurement of PSII density ([eta]PSII) rather than use of an assumed value as changes in [eta]PSII can have a profound impact on light curve parameters. A field study in Fremantle Harbour found a healthy (negligible nutrient starvation), diatom dominated, phytoplankton community. Results suggest that phytoplankton are able to begin boosting photosynthetic capability just prior to morning twilight. Waters in the harbour were well mixed via tidal motion and substantial midday photoinhibition was not observed. Data suggest levels of primary production at the mouth of the harbour are similar to those of coastal waters in the plume of the Ocean Reef wastewater outfall.

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Books on the topic "Chlorophyle a fluorescence"

1

Papageorgiou, George Christos, and Govindjee, eds. Chlorophyll a Fluorescence. Dordrecht: Springer Netherlands, 2004. http://dx.doi.org/10.1007/978-1-4020-3218-9.

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Kalaji, Mohamed H., Vasilij N. Goltsev, Krystyna Zuk-Golaszewska, Marek Zivcak, and Marian Brestic. Chlorophyll Fluorescence, Understanding Crop Performance. 6000 Broken Sound Parkway NW, Suite 300 Boca Raton, FL 33487-2742: CRC Press, 2017. http://dx.doi.org/10.1201/9781315153605.

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DeEll, Jennifer R., and Peter M. A. Toivonen, eds. Practical Applications of Chlorophyll Fluorescence in Plant Biology. Boston, MA: Springer US, 2003. http://dx.doi.org/10.1007/978-1-4615-0415-3.

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Suggett, David J., Ondrej Prášil, and Michael A. Borowitzka, eds. Chlorophyll a Fluorescence in Aquatic Sciences: Methods and Applications. Dordrecht: Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-9268-7.

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K, Lichtenthaler Hartmut, ed. Applications of chlorophyll fluorescence: In photosynthesis research, stress physiology, hydrobiology, and remote sensing. Dordrecht, Netherlands: Kluwer Academic Publishers, 1988.

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Lichtenthaler, Hartmut K., ed. Applications of Chlorophyll Fluorescence in Photosynthesis Research, Stress Physiology, Hydrobiology and Remote Sensing. Dordrecht: Springer Netherlands, 1988. http://dx.doi.org/10.1007/978-94-009-2823-7.

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Lichtenthaler, H. K. Applications of Chlorophyll Fluorescene. Springer, 2011.

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Chlorophyll A Fluorescence A Signature Of Photosynthesis. Springer, 2010.

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C, Papageorgiou George, and Govindjee 1933-, eds. Chlorophyll a fluorescence: A signature of photosynthesis. Dordrecht: Kluwer Academic, 2004.

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Practical Applications of Chlorophyll Fluorescence in Plant Biology. Springer, 2003.

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Book chapters on the topic "Chlorophyle a fluorescence"

1

Strasser, Reto J., Merope Tsimilli-Michael, and Alaka Srivastava. "Analysis of the Chlorophyll a Fluorescence Transient." In Chlorophyll a Fluorescence, 321–62. Dordrecht: Springer Netherlands, 2004. http://dx.doi.org/10.1007/978-1-4020-3218-9_12.

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Nedbal, Ladislav, and John Whitmarsh. "Chlorophyll Fluorescence Imaging of Leaves and Fruits." In Chlorophyll a Fluorescence, 389–407. Dordrecht: Springer Netherlands, 2004. http://dx.doi.org/10.1007/978-1-4020-3218-9_14.

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Cavender-Bares, Jeannine, and Fakhri A. Bazzaz. "From Leaves to Ecosystems: Using Chlorophyll Fluorescence to Assess Photosynthesis and Plant function in Ecological Studies." In Chlorophyll a Fluorescence, 737–55. Dordrecht: Springer Netherlands, 2004. http://dx.doi.org/10.1007/978-1-4020-3218-9_29.

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van Grondelle, Rienk, and Bas Gobets. "Transfer and Trapping of Excitations in Plant Photosystems." In Chlorophyll a Fluorescence, 107–32. Dordrecht: Springer Netherlands, 2004. http://dx.doi.org/10.1007/978-1-4020-3218-9_5.

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Adams, William W., and Barbara Demmig-Adams. "Chlorophyll Fluorescence as a Tool to Monitor Plant Response to the Environment." In Chlorophyll a Fluorescence, 583–604. Dordrecht: Springer Netherlands, 2004. http://dx.doi.org/10.1007/978-1-4020-3218-9_22.

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Lawson, Tracy, and Silvere Vialet-Chabrand. "Chlorophyll Fluorescence Imaging." In Methods in Molecular Biology, 121–40. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7786-4_8.

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Holzwarth, Alfred R. "Time Resolved Chlorophyll Fluorescence." In Applications of Chlorophyll Fluorescence in Photosynthesis Research, Stress Physiology, Hydrobiology and Remote Sensing, 21–31. Dordrecht: Springer Netherlands, 1988. http://dx.doi.org/10.1007/978-94-009-2823-7_3.

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Wassink, E. C. "Chlorophyll Fluorescence and Photosynthesis." In Advances in Enzymology - and Related Areas of Molecular Biology, 91–199. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2006. http://dx.doi.org/10.1002/9780470122563.ch3.

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Senthilkumar, M., N. Amaresan, and A. Sankaranarayanan. "Determination of Chlorophyll Fluorescence." In Springer Protocols Handbooks, 147–48. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-1080-0_38.

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Berden-Zrimec, Maja, Luka Drinovec, and Alexis Zrimec. "Delayed Fluorescence." In Chlorophyll a Fluorescence in Aquatic Sciences: Methods and Applications, 293–309. Dordrecht: Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-9268-7_14.

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Conference papers on the topic "Chlorophyle a fluorescence"

1

Omasa, Kenji. "Image instrumentation of chlorophyll a fluorescence." In Aerospace/Defense Sensing and Controls, edited by Ram M. Narayanan and James E. Kalshoven, Jr. SPIE, 1998. http://dx.doi.org/10.1117/12.312614.

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Gull, Christopher, Minko T. Minkov, Eduardo Gusmao Pereira, and Jose Augusto M. Nacif. "A Low-Cost Chlorophyll Fluorescence Sensor System." In 2016 VI Brazilian Symposium on Computing Systems Engineering (SBESC). IEEE, 2016. http://dx.doi.org/10.1109/sbesc.2016.036.

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Hashemi, Nastaran. "Optofluidic Cytometry on a Chip." In ASME 2012 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/sbc2012-80538.

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The effects of global warming, pollution in river effluents, and changing ocean currents can be studied by characterizing variations in phytoplankton populations. We demonstrate the design and fabrication of a microflow cytometer for characterization of phytoplankton. Guided by chevron-shaped grooves on the top and bottom of a microfluidic channel, two symmetric sheath streams wrap around a central sample stream and hydrodynamically focus it in the center of the channel. The lasers are carefully chosen to provide excitation light close to the maximum absorbance wavelengths for the intrinsic fluorophores chlorophyll and phycoerythrin, and the excitation light is coupled to the flow cytometer through the use of an optical fiber. Fluorescence and light scatter are collected using two multimode optical fibers placed at 90-degree angles with respect to the excitation fiber. Light emerging from these collection fibers is directed through optical bandpass filters into photomultiplier tubes. The cytometer measured the optical and side scatter properties of Karenia b., Synechococcus sp., Pseudo-Nitzchia, Alexandrium, Nitzschia, and Thallassiosira pseudonana. The microflow cytometer proved sensitive enough to detect and characterize picoplankton with diameter approximately 1 μm and larger phytoplankton of up to 80 μm in length. The wide range in size discrimination coupled with detection of intrinsic fluorescent pigments suggests that this microflow cytometer will be able to distinguish different populations of phytoplankton on unmanned underwater vehicles. We also studied the effect of the sheath-to-sample flow-rate ratio on the light scatter and fluorescence of these marine microorganisms.

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Daley, Paul F., J. Timothy Ball, Joseph A. Berry, Juergen Patzke, and Klaus E. Raschke. "Visualizing photosynthesis through processing of chlorophyll fluorescence images." In Electronic Imaging '90, Santa Clara, 11-16 Feb'93, edited by Alan C. Bovik and William E. Higgins. SPIE, 1990. http://dx.doi.org/10.1117/12.19560.

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Marra, John. "Diurnal variability in chlorophyll fluorescence: observations and modeling." In San Diego '92, edited by Gary D. Gilbert. SPIE, 1992. http://dx.doi.org/10.1117/12.140654.

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Zhou, Lina, Shuchao Cheng, and Haiye Yu. "Detection of Chlorophyll Content of Rice Leaves by Chlorophyll Fluorescence Spectrum Based on PCA-ANN." In 2016 7th International Conference on Mechatronics, Control and Materials (ICMCM 2016). Paris, France: Atlantis Press, 2016. http://dx.doi.org/10.2991/icmcm-16.2016.12.

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Ivanova, M. V., and G. G. Suvorova. "Study of coniferous chlorophyll fluorescence parameters under vegetation conditions." In IX Congress of society physiologists of plants of Russia "Plant physiology is the basis for creating plants of the future". Kazan University Press, 2019. http://dx.doi.org/10.26907/978-5-00130-204-9-2019-187.

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Damm, Alexander, Micol Rossini, Roberto Colombo, Uwe Rascher, and Michael E. Schaepman. "Airborne based spectroscopy to measure sun-induced chlorophyll fluorescence." In 2014 6th Workshop on Hyperspectral Image and Signal Processing: Evolution in Remote Sensing (WHISPERS). IEEE, 2014. http://dx.doi.org/10.1109/whispers.2014.8077628.

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Li, Cuiling, Xiu Wang, and Zhijun Meng. "Tomato seeds maturity detection system based on chlorophyll fluorescence." In SPIE/COS Photonics Asia, edited by Yongtian Wang, Tina E. Kidger, and Kimio Tatsuno. SPIE, 2016. http://dx.doi.org/10.1117/12.2247866.

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Kashulin, P. A., and N. V. Kalacheva. "Physiological responses in plants induced by artificially generated EMF vector potential." In Physics of Auroral Phenomena. FRC KSC RAS, 2020. http://dx.doi.org/10.37614/2588-0039.2020.43.042.

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The theoretically suggested eigen activity of EMF vector-potential (VP) was checked up through finding out its plausible bioactivity in relation to living plant objects. The stimulating effects of steady-state artificially generated by toroidal emitter VPof magnetic field on Taraxacum officinaledormant or germinated seeds and on Avena sativacoleoptiles are registered. The modulation of photosynthetic activity of underwent to VP emission Hibiscus rosa-sinensisplants in terms of its chlorophyll red fluorescence was shown as well. The findings support the theoretical premises regarding an eigen physical reality of VP.

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Reports on the topic "Chlorophyle a fluorescence"

1

Frost, Bruce. Time- and Irradiance-Dependent Behavior of the Quantum Yield of Chlorophyll alpha Fluorescence. Fort Belvoir, VA: Defense Technical Information Center, September 2001. http://dx.doi.org/10.21236/ada627704.

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Perry, Mary J. Time- and Irradiance-Dependent Behavior of the Quantum Yield of Chlorophyll alpha Fluorescence. Fort Belvoir, VA: Defense Technical Information Center, August 2001. http://dx.doi.org/10.21236/ada628016.

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Perry, Mary J. Determination of Phytoplankton Biomass in Coastal Waters by Remote Sensing of Chlorophyll A Fluorescence (AASERT). Fort Belvoir, VA: Defense Technical Information Center, September 1997. http://dx.doi.org/10.21236/ada635376.

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4

Medeiros, W. H., and C. D. Wirick. SEEP II, Shelf Edge Exchange Processes-II: Chlorophyll a fluorescence, temperature, and beam attenuation measurements from moored fluorometers. Office of Scientific and Technical Information (OSTI), February 1992. http://dx.doi.org/10.2172/10140332.

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Medeiros, W. H., and C. D. Wirick. SEEP II, Shelf Edge Exchange Processes-II: Chlorophyll a fluorescence, temperature, and beam attenuation measurements from moored fluorometers. Office of Scientific and Technical Information (OSTI), February 1992. http://dx.doi.org/10.2172/5406681.

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Mueller, J., and R. E. Lange. Bio/Optical Provinces of the Northeast Pacific Ocean in Summer: A provisional Analysis Combining Remotely Sensed Ocean Color with Irradiance and Chlorophyll-A Fluorescence Profiles. Fort Belvoir, VA: Defense Technical Information Center, January 1988. http://dx.doi.org/10.21236/ada232127.

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