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Journal articles on the topic 'Chlorotic ringspots'

Author: Grafiati

Published: 4 June 2021

Last updated: 18 February 2022

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1

Chen, Y. K., and Y. H. Hong. "First Report of Begonia Chlorotic Ringspot Caused by Zucchini yellow mosaic virus in Taiwan." Plant Disease 92, no. 8 (2008): 1247. http://dx.doi.org/10.1094/pdis-92-8-1247b.

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Begonia (Begonia semperflorens) showing symptoms of systemic chlorotic ringspots were observed in the central part of Taiwan in May 2006. Infected begonia plants showed faint ringspots in leaves at the early stage of infection. Symptoms turned chlorotic and coalesced as the disease progressed. Electron microscopic examination revealed filamentous virus particles approximately 750 × 13 nm in the crude sap of infected begonia. Typical pinwheel inclusion bodies of potyvirus infection were observed in the ultrathin sections of infected begonia. A virus culture was isolated via mechanical inoculati

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2

Rehanek, Marius, Susanne von Bargen, Martina Bandte, David G. Karlin, and Carmen Büttner. "A novel emaravirus comprising five RNA segments is associated with ringspot disease in oak." Archives of Virology 166, no. 3 (2021): 987–90. http://dx.doi.org/10.1007/s00705-021-04955-w.

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AbstractWe report the complete nucleotide sequence of the genome of a novel virus in ringspot-diseased common oak (Quercus robur L.). The newly identified pathogen is associated with leaf symptoms such as mottle, chlorotic spots and ringspots on diseased trees. High-throughput sequencing (HTS, Illumina RNASeq) was used to explore the virome of a ringspot-diseased oak that had chlorotic ringspots of suspected viral origin on leaves for several years. Bioinformatic analysis of the HTS dataset followed by RT-PCR enabled us to determine complete sequences of four RNA genome segments of a novel vir

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3

Kiambi, Roy G., Mattie M. Baumann, and Benham E. Lockhart. "A New Disease of Epimedium Caused by Carnation Ringspot Virus." Plant Health Progress 19, no. 4 (2018): 329–31. http://dx.doi.org/10.1094/php-09-18-0051-br.

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Epimediums (Epimedium grandiflorum) are popular perennial groundcovers and are known to produce a wide range of medicinal effects. Extracts have long been used in traditional Chinese medicine. Carnation ringspot virus (CRSV) has a wide host range. In 2018, symptoms were observed on epimedium in three different landscape settings across St. Paul, MN. Leaf symptoms consisted of conspicuous mottling, chlorotic ringspots, and distortion of leaf margins. CRSV was confirmed by reverse transcription polymerase chain reaction. The CRSV host range now includes epimedium, which is the first species of t

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4

Holcomb, G. E., and R. A. Valverde. "Natural Infection of Salvia uliginosa with Cucumber Mosaic Cucumovirus." HortScience 33, no. 7 (1998): 1215–16. http://dx.doi.org/10.21273/hortsci.33.7.1215.

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Salvia uliginosa Benth. plants, in an experimental planting of Salvia species, exhibited virus-like symptoms of chlorotic line patterns and ringspots. The suspect virus was mechanically transmitted to several experimental hosts and was identified as cucumber mosaic cucumovirus (CMV) based on dsRNA gel patterns, positive reaction with CMV antiserum, and particle morphology as observed by transmission electron microscopy.

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5

Zheng, You-Xiu, Ching-Chung Chen, Chia-Jin Yang, Shyi-Dong Yeh, and Fuh-Jyh Jan. "Identification and characterization of a tospovirus causing chlorotic ringspots on Phalaenopsis orchids." European Journal of Plant Pathology 120, no. 2 (2007): 199–209. http://dx.doi.org/10.1007/s10658-007-9208-7.

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6

Roggero, P., M. Ciuffo, G. Dellavalle, P. Gotta, S. Gallo, and D. Peters. "Additional Ornamental Species as Hosts of Impatiens Necrotic Spot Tospovirus in Italy." Plant Disease 83, no. 10 (1999): 967. http://dx.doi.org/10.1094/pdis.1999.83.10.967a.

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Impatiens necrotic spot (INSV) and tomato spotted wilt (TSWV) tospoviruses are among the most important viral pathogens of glasshouse ornamental plants worldwide (1). Tospovirus infections drastically reduce the market value of plants and create certification problems for international traders. As with TSWV, the number of natural host species recorded for INSV is steadily increasing (2). In 1998, severe INSV infections were found on different ornamental plants in glasshouses in the Piedmont Region of northwestern Italy, together with heavy infestations of the thrips Frankliniella occidentalis.

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7

Olmedo-Velarde, Alejandro, Adam C. Park, Jari Sugano, et al. "Characterization of Ti Ringspot-Associated Virus, a Novel Emaravirus Associated with an Emerging Ringspot Disease of Cordyline fruticosa." Plant Disease 103, no. 9 (2019): 2345–52. http://dx.doi.org/10.1094/pdis-09-18-1513-re.

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Ti ringspot is an emerging foliar disease of the ti plant (Cordyline fruticosa) in Hawaii that is quickly spreading throughout the islands. Symptoms include small chlorotic ringspots on leaves that often coalesce to form larger lesions. Although several virus species have been discovered in symptomatic plants, none have been associated with these symptoms. Here, we report and characterize a novel virus closely associated with ti ringspot symptoms in Hawaii. The presence of double membrane bodies approximately 85 nm in diameter in symptomatic cells and sequence analyses of five genomic RNA segm

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8

McGovern, R. J., J. E. Polston, and B. K. Harbaugh. "Detection of a Severe Isolate of Impatiens Necrotic Spot Virus Infecting Lisianthus in Florida." Plant Disease 81, no. 11 (1997): 1334. http://dx.doi.org/10.1094/pdis.1997.81.11.1334b.

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In May 1997, inclusions typical of a tospovirus were visualized by light microscopy in leaf tissue of lisianthus (Eustoma grandiflorum) exhibiting stunting, necrotic ringspots, leaf distortion, and systemic necrosis. Wilting and plant death were the final symptoms observed. Affected plants occurred at low incidence (<0.1%) in greenhouse-grown lisianthus in Manatee County, FL. Symptomatic tissue tested positive for impatiens necrotic spot virus (INSV) and negative for tomato spotted wilt virus (TSWV) with enzyme-linked immunosorbent assay (ELISA; Agdia, Elkhart, IN). Mechanical transmission

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9

de Jensen, C. E., and S. Adkins. "First Report of Tomato chlorotic spot virus in Lettuce in Puerto Rico." Plant Disease 98, no. 7 (2014): 1015. http://dx.doi.org/10.1094/pdis-12-13-1200-pdn.

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Viral diseases have not previously been described in lettuce (Lactuca sativa) in Puerto Rico. In April 2013, lettuce samples from a hydroponic greenhouse in Guayanilla were submitted to the Plant Disease Clinic at the University of Puerto Rico's Juana Díaz Experimental Station. Lettuce plants were symptomatic for virus and had thrips. Eight samples reacted with Tomato spotted wilt virus (TSWV) DAS-ELISA (Envirologix, Portland, ME) and lateral flow immunoassay (Envirologix). Further sampling at the hydroponic greenhouse, which had 45,000 lettuce plants in different growth stages, revealed leaf

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10

Chen, X. J., Y. Huang, J. Li, et al. "First Report of Impatiens necrotic spot virus Causing Chlorotic Ringspots on Pepper in Yunnan, China." Plant Disease 100, no. 5 (2016): 1029. http://dx.doi.org/10.1094/pdis-10-15-1178-pdn.

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11

Luis-Arteaga, M., and M. A. Cambra. "First Report of Natural Infection of Greenhouse-Grown Tomato and Weed Species by Pelargonium zonate spot virus in Spain." Plant Disease 84, no. 7 (2000): 807. http://dx.doi.org/10.1094/pdis.2000.84.7.807c.

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Tomato (Lycopersicon esculentum Mill.) plants showing severe chlorotic and necrotic ringspots, line patterns on leaves, and concentric chlorotic ringspots on stems and fruits were observed in plastic greenhouse-grown tomato crops cv. Royesta during the spring of 1996 in Zaragoza province, Northeast Spain. Symptoms were similar to those associated with Pelargonium zonate spot virus (PZSV) infection on tomato in Italy (1,2). The causal agent was mechanically transmitted from leaf, fruit, and stem samples to several indicator species. The following host reactions were recorded: chlorotic local le

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12

Tang, J., D. R. Elliott, B. D. Quinn, G. R. G. Clover, and B. J. R. Alexander. "Occurrence of Hibiscus chlorotic ringspot virus in Hibiscus spp. in New Zealand." Plant Disease 92, no. 9 (2008): 1367. http://dx.doi.org/10.1094/pdis-92-9-1367a.

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Hibiscus spp. are popular ornamental plants in New Zealand. The genus is susceptible to Hibiscus chlorotic ringspot virus (HCRSV), a member of the genus Carmovirus, which has been reported in Australia, El Salvador, Singapore, the South Pacific Islands, Taiwan, Thailand, and the United States (1–4). In May of 2004, chlorotic spotting and ringspots were observed on the leaves of two H. rosa-sinensis plants in a home garden in Auckland, New Zealand. When inoculated with sap from symptomatic leaves, Chenopodium quinoa and C. amaranticolor developed faint chlorotic local lesions 12 to 15 days late

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13

Bau, Huey-Jiunn, Ying-Huey Cheng, Tsong-Ann Yu, et al. "Field Evaluation of Transgenic Papaya Lines Carrying the Coat Protein Gene of Papaya ringspot virus in Taiwan." Plant Disease 88, no. 6 (2004): 594–99. http://dx.doi.org/10.1094/pdis.2004.88.6.594.

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Four transgenic papaya lines expressing the coat protein (CP) gene of Papaya ringspot virus (PRSV) were evaluated under field conditions for their reaction to PRSV infection and fruit production in 1996 to 1999. Plants were exposed to natural virus inoculation by aphids in two adjacent fields in four different plantings at the same sites. None of the transgenic lines showed severe symptoms of PRSV whereas control nontransgenic plants were 100% severely infected 3 to 5 months after planting. In the first and second trials, 20 to 30% of the transgenic plants showed mild symptoms consisting of co

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14

Materazzi, A., and E. Triolo. "Spathiphyllum sp.: A New Natural Host of Impatiens necrotic spot virus." Plant Disease 85, no. 4 (2001): 448. http://dx.doi.org/10.1094/pdis.2001.85.4.448b.

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In September 1999, several Spathiphyllum plants grown in a greenhouse in Tuscany (Italy) showed leaf symptoms in the form of concentric chlorotic ringspots, line patterns, and irregular chlorotic blotches. These symptoms developed into localized necrosis. Crude sap of tissues showing symptoms was mechanically inoculated to young symptomless Spathiphyllum plants and to Nicotiana benthamiana and N. clevelandii. Samples drawn from symptomatic and symptomless tissues of naturally or artificially infected Spathiphyllum and Nicotiana plants were tested for the presence of Alfalfa mosaic virus (AMV),

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15

Chen, Y. K., Y. S. Chang, Y. W. Lin, and M. Y. Wu. "First Report of Cucumber mosaic virus in Desert Rose in Taiwan." Plant Disease 96, no. 4 (2012): 593. http://dx.doi.org/10.1094/pdis-11-11-0917.

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Desert rose (Adenium obesum (Forssk.) Roem. & Schult, family Apocynaceae) is native to southeastern Africa, and is a perennial potted ornamental with colorful flowers that are popular in Taiwan. Symptoms of mosaic and chlorotic ringspots and line patterns on leaves were observed in July 2010, on all eight plants in a private garden in Potzu, Chiayi, Taiwan. Spherical virus particles with a diameter of approximately 28 nm were observed in crude sap prepared from symptomatic leaves. Virus culture was established by successive local lesion isolation in Chenopodium quinoa and was maintained in

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16

Waterworth, H. E. "First Report of Tobacco Streak Ilarvirus from Honeysuckle." Plant Disease 82, no. 12 (1998): 1402. http://dx.doi.org/10.1094/pdis.1998.82.12.1402b.

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A honeysuckle (Lonicera fragrantissima) shrub on the grounds of the former Plant Quarantine Station, Glenn Dale, MD, had chlorotic leaves on some shoot tips and a mild veinal chlorosis. Young leaves were triturated in buffer and rub-inoculated onto a series of potential indicator hosts. The virus incited necrotic local lesions and necrosis of the growing point in Chenopodium quinoa, etched ringspots on inoculated leaves of Nicotiana tabacum Xanthi nc, mosaic in Zinnia violacea, and chlorotic local lesions in Tetragonia tetragonioides. It did not infect any of 46 other herbaceous genera in fami

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17

Eisold, Anne-Mareen Ellen. "Molecular Characterization of the Movement and Coat Proteins of a New Elm Mottle Virus Isolate Infecting European White Elm (Ulmus laevis Pall.)." International Journal of Phytopathology 8, no. 1 (2019): 01–07. http://dx.doi.org/10.33687/phytopath.008.01.2742.

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European white elms (Ulmus laevis Pall.) growing in a park in Caputh near Berlin (Germany) were regularly monitored over a period of 18 years showing virus infection-like symptoms such as chloroses, chlorotic ringspots, mottling and dieback. To obtain the evidence for viral infection, RNA-seq using an Illumina Hi Seq2500 was conducted and three contigs were obtained. They match with the three EMoV genomic RNAs and cover the open reading frames for the viral replicase, the polymerase and the movement and coat proteins (MP, CP). The contigs show identities of 95.3–96.4%, 91.9–93.3% and 89.0–92.5

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18

Nunes, M. A., C. A. L. de Oliveira, M. L. de Oliveira, et al. "Transmission of Citrus leprosis virus C by Brevipalpus phoenicis (Geijskes) to Alternative Host Plants Found in Citrus Orchards." Plant Disease 96, no. 7 (2012): 968–72. http://dx.doi.org/10.1094/pdis-06-11-0538.

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The equivalent of US$75 million is spent each year in Brazil to control Brevipalpus phoenicis, a mite vector of Citrus leprosis virus C (CiLV-C). In this study, we investigated the possibility that hedgerows and windbreaks normally found in citrus orchards could host CiLV-C. Mites confined by an adhesive barrier were reared on sweet orange fruit with leprosis symptoms then were transferred to leaves of Hibiscus rosa-sinensis, Malvaviscus arboreus, Grevilea robusta, Bixa orellana, and Citrus sinensis. Ninety days post infestation, the descendant mites were transferred to Pera sweet orange plant

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19

Baysal-Gurel, F., R. Li, K. S. Ling, and S. A. Miller. "First Report of Tomato chlorotic spot virus Infecting Tomatoes in Ohio." Plant Disease 99, no. 1 (2015): 163. http://dx.doi.org/10.1094/pdis-06-14-0639-pdn.

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Virus-like symptoms including deformation, discoloration, and necrotic ringspots on green and red fruits of tomato (Solanum lycopersicum L. cv. Big Dena) were observed in a 400 m2 commercial high tunnel in Wayne Co., Ohio, in July and August 2013. No symptoms were observed on leaves. Incidence of symptomatic fruits was approximately 15%. Tomato seedlings transplanted into the high tunnel were produced in a greenhouse containing ornamental plants. The grower observed high levels of thrips infestation in the tomato seedlings prior to transplanting. A tospovirus was suspected as a possible causal

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20

Batuman, O., M. R. Rojas, A. Almanzar, and R. L. Gilbertson. "First Report of Tomato chlorotic spot virus in Processing Tomatoes in the Dominican Republic." Plant Disease 98, no. 2 (2014): 286. http://dx.doi.org/10.1094/pdis-07-13-0685-pdn.

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Processing tomatoes (Solanum lycopersicum) are an important industry in the Dominican Republic. In November 2012, symptoms typical of tospovirus infection (bronzing, chlorosis, and necrosis of leaves) appeared in numerous processing tomato fields in the North (>50% incidence in some fields) and a few fields in the South (<1% incidence). Plants in affected fields had large populations of thrips on leaves and in flowers. Symptomatic leaves from four fields in the North (Guayubin, Juan Gomez, Hatillo Palma, and Navarrete) and one field in the South (Azua) were positive for infection by Toma

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21

Subbaiah, K. Venkata, D. V. R. Sai Gopal, and M. Krishna Reddy. "First Report of a Tospovirus on Sunflower (Helianthus annus L.) from India." Plant Disease 84, no. 12 (2000): 1343. http://dx.doi.org/10.1094/pdis.2000.84.12.1343b.

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Virus-like symptoms were observed on sunflower in and around Tirupati during January 1998. Infected plants exhibited severe mosaic, systemic necrosis along the stem and floral heads, leaf distortion, and ringspots on leaves. The causal virus, mechanically transmissible from sunflower to sunflower cvs. Morden, MHSF8, MHSF18, KBHS1, and Cargil, developed symptoms like those in the original plant. The virus caused chlorotic and necrotic spots on Chenopodium amaranticolor, chlorotic and necrotic rings on cowpea cv. C-152, chlorotic spots on Datura metal and Petunia hybrida, chlorotic rings and sys

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22

Baker, C. A., and S. Adkins. "Tobacco ringspot virus Found in the Cardboard Cycad (Zamia furfuracea) in Florida." Plant Disease 91, no. 1 (2007): 112. http://dx.doi.org/10.1094/pd-91-0112b.

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Zamia furfuracea (Zamiaceae) is native of coastal Mexico. It is a popular houseplant and easy to grow outdoors in warm climates. In November 2005, a plant of Z. furfuracea, originally from Texas, was received at the Division of Plant Industry in Gainesville, FL. The plant had numerous chlorotic spots on the leaves that eventually became necrotic. Leaves were ground in phosphate buffer (pH 7.2) with Carborundum and used to inoculate a host range that included Chenopodium amaranticolor, C. quinoa, Gomphrena globosa, Datura stramonium, and Nicotiana benthamiana. Systemic symptoms were seen in C.

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23

Cardin, L., J. P. Onesto, and B. Moury. "First Report of Alfalfa mosaic virus in Physostegia virginiana." Plant Disease 86, no. 1 (2002): 72. http://dx.doi.org/10.1094/pdis.2002.86.1.72d.

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Physostegia virginiana Benth. (false dragon head) is a perennial plant from the family Lamiaceae cultivated as an ornamental in gardens and for cut-flower production. In 2000, stunting of plants and yellow-to-brown ringspots on leaves were observed in cut-flower production in the Alpes Maritimes Department (southeast France). These symptoms greatly decreased the commercial value of the stems. The disease was attributed to Alfalfa mosaic virus (AMV) because extracts of infected plant tissues revealed typical bacilliform particles by electron microscopy, produced symptoms typical of AMV after in

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24

Gillaspie, A. G., and S. A. Ghabrial. "First Report of Peanut Stunt Cucumovirus Naturally Infecting Desmodium sp." Plant Disease 82, no. 12 (1998): 1402. http://dx.doi.org/10.1094/pdis.1998.82.12.1402a.

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Plant species in the genus Desmodium (Fabaceae) are used as forage and cover crops and include a number of common weeds such as beggarweed (D. tortuosum) and beggarlice (D. intortum). Accessions of the genus are part of the plant genetic resources collection maintained at Griffin, GA. Peanut stunt cucumovirus (PSV) was detected in naturally infected plants of Desmodium sp. PI 322505 (from Brazil) in a germ plasm regeneration plot by a direct antigen coating-enzyme-linked immunosorbent assay (DAC-ELISA) with an antiserum against PSV strain ER (subgroup I) originally isolated from cowpea in Geor

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25

Kucharek, T. A., D. E. Purcifull, R. G. Christie, and K. D. Perkins. "The Association of Severe Epidemics of Cucumber Mosaic in Commercial Fields of Pepper and Tobacco in North Florida with Inoculum in Commelina benghalensis and C. communis." Plant Disease 82, no. 10 (1998): 1172. http://dx.doi.org/10.1094/pdis.1998.82.10.1172a.

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Since 1995, severe epidemics of cucumber mosaic virus (CMV) have occurred in select fields of tobacco (Nicotiana tabacum) and pepper (Capsicum annuum) in three counties in northern Florida. Yield losses greater than 50% have occurred in both crops. Baker and Zettler (1) identified the presence of CMV in one plant of tropical spiderwort (Commelina benghalensis) in an organic garden on the campus of the University of Florida 10 years ago. In addition, they infected tropical spiderwort and Asiatic dayflower (Commelina communis) with isolates of CMV. Since 1995, in one area of northern Alachua Cou

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Lemmetty, A., S. Latvala, A. T. Jones, P. Susi, W. J. McGavin, and K. Lehto. "Purification and Properties of a New Virus from Black Currant, Its Affinities with Nepoviruses, and Its Close Association with Black Currant Reversion Disease." Phytopathology® 87, no. 4 (1997): 404–13. http://dx.doi.org/10.1094/phyto.1997.87.4.404.

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Black currant reversion is a virus-like disease whose causal agent has not been identified. In rooted cuttings of a black currant plant affected with the severe form of the disease, pronounced chlorotic line patterns and ringspots developed in newly emerging leaves. From such symptom-bearing leaves, a virus was mechanically transmitted with difficulty to Chenopodium quinoa and, from this host, to other herbaceous test plants. The virus was purified and partially characterized, and the purified viri-ons were used for antiserum production. Virus particles were isometric, approximately 27 nm in d

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27

Escriu, F., M. A. Cambra, and M. Luis-Arteaga. "First Report of Pepper as a Natural Host for Pelargonium zonate spot virus in Spain." Plant Disease 93, no. 12 (2009): 1346. http://dx.doi.org/10.1094/pdis-93-12-1346b.

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Pelargonium zonate spot virus (PZSV) was first reported on Pelargonium zonale (L.) L'Hér. ex Aiton and later on tomato in Italy, Spain, France (1), and the United States (2). In Spain, PZSV was first detected in 1996 in tomato plants of cv. Royesta from greenhouses in Zaragoza Province (3) and subsequently in tomato in the Catalonia and Navarra areas. In April 2006, symptoms of PZSV were found at high incidence on tomato in a greenhouse in Huesca, Aragón (northeastern Spain). Randomly distributed pepper plants (Capsicum annuum L.) of cv. Estilo F1 growing in the same greenhouse showed severe f

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Soto-Valladares, A. G., R. De La Torre-Almaraz, B. Xoconostle-Cazares, and R. Ruíz-Medrano. "First Report of Cymbidium mosaic virus and Odontoglossum ringspot virus in Orchids in Mexico." Plant Disease 96, no. 3 (2012): 464. http://dx.doi.org/10.1094/pdis-08-11-0655.

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In 2010, a survey for viral diseases in commercial, orchid-producing greenhouses was carried out in Morelos, Mexico. Many symptomatic plants were observed. The most common leaf symptoms were yellow mottle, yellow streaks, and chlorotic and necrotic ringspots. Leaf samples were collected from eight symptomatic plants from the following genera: Encyclia, Oncidium, Shomburghia, Brassia, Guarianthe, Cattleya, Epidendrum, Vanilla, Xilobium, Laelia, and Brassocattleya. Samples were tested using double-antibody sandwich (DAS)-ELISA (Agdia, Elkhart, IN) with antiserum for Cymbidium mosaic virus (CymMV

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Adkins, S., L. Breman, C. A. Baker, and S. Wilson. "First Report of Tomato spotted wilt virus in Blackberry Lily in North America." Plant Disease 87, no. 1 (2003): 102. http://dx.doi.org/10.1094/pdis.2003.87.1.102c.

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Blackberry lily (Belamcanda chinensis (L.) DC.) is an herbaceous perennial in the Iridaceae characterized by purple-spotted orange flowers followed by persistent clusters of black fruit. In July 2002, virus-like symptoms including chlorotic ringspots and ring patterns were observed on blackberry lily leaves on 2 of 10 plants in a south Florida ornamental demonstration garden. Inclusion body morphology suggested the presence of a Tospovirus. Tomato spotted wilt virus (TSWV) was specifically identified by serological testing using enzyme-linked immunosorbent assay (Agdia, Elkhart, IN). Sequence

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Cardin, L., and B. Moury. "First Report of Cucumber mosaic virus in Viola hederacea in France and Italy." Plant Disease 91, no. 3 (2007): 331. http://dx.doi.org/10.1094/pdis-91-3-0331a.

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Viola hederacea Labill. (Australian violet or trailing violet), family Violaceae, is native to eastern Australia and used for its ornamental quality in humid conditions, especially in terrariums. Mosaic and chlorotic ringspots associated with a mild crinkling on leaves of V. hederacea were observed in gardens in southeast France and La Mortola, Italy in 2001 and 2003, respectively. These symptoms were different from the small chlorotic spots reported in the same species in Queensland, Australia, from which a rhabdovirus was isolated (1). In samples collected from both locations, presence of Cu

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Cardin, L., J. P. Onesto, and B. Moury. "First Report of Cucumber mosaic virus in Paeonia lactifera in France." Plant Disease 94, no. 6 (2010): 790. http://dx.doi.org/10.1094/pdis-94-6-0790c.

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Chinese peony (Paeonia lactiflora Pall.), a hardy ornamental plant of the family Paeoniaceae cultivated in gardens and for cut flower production, is frequently infected by Tobacco rattle virus (TRV) in the field. The virus usually induces severe mosaic and chlorotic ringspot symptoms in the leaves, decreasing the commercial value of cut flowers. TRV is routinely detected by mechanical inoculation onto Nicotiana tabacum cv Xanthi, where it induces typical necrotic local ringspots in 3 to 7 days, followed by a reverse transcription (RT)-PCR test (2). In 2004, Xanthi test plants inoculated with s

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Vargas-Asencio, J., H. McLane, E. Bush, and K. L. Perry. "Spinach latent virus Infecting Tomato in Virginia, United States." Plant Disease 97, no. 12 (2013): 1663. http://dx.doi.org/10.1094/pdis-05-13-0529-pdn.

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Plants in a single field of commercial tomato (Solanum lycopersicum) of unidentified cultivars in Virginia in July, 2012, were observed showing stunting, leaf distortion, twisting and thickening, discoloration, and color streaking and ringspots on fruits. Serological tests were negative for Cucumber mosaic virus, Groundnut ringspot virus, Tomato spotted wilt virus, Tomato chlorotic spot virus, Impatiens necrotic spot virus, Tobacco mosaic virus, and Tomato bushy stunt virus (Agdia, Inc., Elkhart, IN). Using a membrane-based macroarray (3), hybridization was observed to 8 of 9 70-mer oligonucle

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Bandte, Martina, Marius Rehanek, Bertram Leder, Susanne von Bargen, and Carmen Büttner. "Identification of an Emaravirus in a Common Oak (Quercus robur L.) Conservation Seed Orchard in Germany: Implications for Oak Health." Forests 11, no. 11 (2020): 1174. http://dx.doi.org/10.3390/f11111174.

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We observed the health status of oak trees in a conservation seed orchard for over twenty years, focusing on characteristic virus-suspected symptoms. The orchard was established in 1992 in Kreuztal, North Rhine-Westphalia (Germany) with 1302 seedlings in 186 clusters. The number of seedlings showing chlorotic ringspots and mottle on leaves has fluctuated annually, but has increased from 3.3% to 12.1% in the last 20 years; the number of affected clusters has risen from 8% to 25.9%. A scientific breakthrough was the identification of a novel virus related to members of the genus Emaravirus in di

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Gera, A., J. Cohen, R. Salomon, and B. Raccah. "Iris Yellow Spot Tospovirus Detected in Onion (Allium cepa) in Israel." Plant Disease 82, no. 1 (1998): 127. http://dx.doi.org/10.1094/pdis.1998.82.1.127b.

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During March 1997, 20 to 30% of field-grown onion (Allium cepa), observed in Bet Shean Valley, Israel, had unusual viral symptoms of straw-colored ringspots on leaves and flower stalks. Leaf samples were analyzed by transmission electron microscopy (EM) of leaf dip preparations. Typical tospovirus-like particles were observed only with samples taken from symptomatic plants. Crude sap from symptomatic tissue was mechanically transmitted to Nicotiana benthamiana, Chenopodium quinoa, and Gomphrena globosa. On inoculated plants of N. benthamiana, chlorotic spots developed on inoculated leaves, fol

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Meng, J. R., P. P. Liu, C. W. Zou, et al. "First Report of a Tospovirus in Mulberry." Plant Disease 97, no. 7 (2013): 1001. http://dx.doi.org/10.1094/pdis-08-12-0792-pdn.

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Mulberry (Morus alba L.) is an economically important crop grown widely throughout Asia. Various virus-like symptoms including mosaics, vein banding, and chlorotic ringspots have been observed and reported on mulberry trees in China and Japan for decades. However, the etiology of mulberry viral diseases is generally understudied, although two mulberry-infecting viruses, Mulberry latent virus (genus Carlavirus) (2) and Mulberry ringspot virus (genus Nepovirus) (3), have been partially characterized. In a recent (2010 to 2011) field survey in Guangxi Province, China, supported by the local gover

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Šubíková, V., E. Kollerová, and Ľ. Slováková. "Occurrence of nepoviruses in small fruits and fruit trees in Slovakia." Plant Protection Science 38, SI 2 - 6th Conf EFPP 2002 (2017): 367–69. http://dx.doi.org/10.17221/10493-pps.

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A survey of nepoviruses in small fruits and fruit trees in thirteen localities of Slovakia was undertaken during the last three years. The samples of spontaneously infected small fruits and fruit trees with symptoms such as leaf yellowing, mottling, chlorotic spotting, vein clearing, chlorosis, dwarfing, and in some cases even plant dying, were analysed. Virus identification in collected samples from affected crops was performed by ELISA using polyclonal antibodies. The following quarantine nepoviruses were detected: Tobacco ringspot nepovirus, Tomato ringspot nepovirus, Tomato black ring nepo

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Puffinberger, C. W. "Euonymus Chlorotic Ringspot Disease Caused by Tomato Ringspot Virus." Phytopathology 75, no. 4 (1985): 423. http://dx.doi.org/10.1094/phyto-75-423.

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Poudel, B., T. Ho, A. Khadgi, and I. E. Tzanetakis. "EPIDEMIOLOGY OF BLACKBERRY CHLOROTIC RINGSPOT VIRUS." Acta Horticulturae, no. 1064 (January 2015): 311–16. http://dx.doi.org/10.17660/actahortic.2015.1064.38.

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Poudel, Bindu, Thien Ho, Alma Laney, Archana Khadgi, and Ioannis E. Tzanetakis. "Epidemiology of Blackberry chlorotic ringspot virus." Plant Disease 98, no. 4 (2014): 547–50. http://dx.doi.org/10.1094/pdis-08-13-0866-re.

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The pollen- and seed-borne ilarviruses pose a substantial threat to many specialty crops, including berries, rose, and tree fruit, because there are no efficient control measures other than avoidance. The case of Blackberry chlorotic ringspot virus (BCRV) is of particular interest because the virus has been found to be an integral part of blackberry yellow vein disease and is widespread in rose plants affected by rose rosette disease. This study provides insight into the epidemiology of BCRV, including incidence in blackberry and rose; host range, with the addition of apple as a host of the vi

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Cardin, L., B. Delecolle, and B. Moury. "Occurrence of Mosaic Caused by Cucumber mosaic virus in Lobelia Hybrids in France and Italy." Plant Disease 89, no. 3 (2005): 340. http://dx.doi.org/10.1094/pd-89-0340b.

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In 2002, mosaic symptoms associated with yellowish ringspots were observed on leaves of a hybrid of lobelia (Lobelia spp.) grown in a public garden in Alsace (France). In 2003, similar symptoms were observed in Lobelia laxiflora in the Hanbury botanical garden (La Mortola, Italy) and the botanical garden of Nice (France). Cucumber mosaic virus (CMV) was identified in samples collected from the three locations on the basis of the following: symptoms exhibited by a host range of inoculated plants previously described (1); the observation of isometric particles (approximately 30 nm) with an elect

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Aboughanem-Sabanadzovic, N., T. W. Allen, M. Broome, A. Lawrence, W. F. Moore, and S. Sabanadzovic. "First Report of Kudzu (Pueraria montana) Infections by Tobacco ringspot virus in Mississippi." Plant Disease 98, no. 12 (2014): 1746. http://dx.doi.org/10.1094/pdis-12-13-1249-pdn.

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Virus-like symptoms were observed in several kudzu patches in Mississippi during a survey of viruses infecting soybean carried out in late summer/fall of 2013 as a part of a project funded by the Mississippi Soybean Promotion Board. Symptomatology consisted of chlorotic mottle and ringspots, vein-associated feathering, necrosis, and leaf deformation, which were often observed in combination on the same plant. In order to identify the virus(es) involved in the disease, young leaves from a symptomatic kudzu sample collected in Kemper County were crushed in 10 volumes of 0.1 M phosphate buffer (p

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Verma, Raj, Satya Prakash, and S. P. S. Tomer. "First Report of Zucchini yellow mosaic virus in Cucumber (Cucumis sativus) in India." Plant Disease 88, no. 8 (2004): 906. http://dx.doi.org/10.1094/pdis.2004.88.8.906b.

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In August of 2002, cucumber (Cucumis sativus L. cv. Himangi) plants grown in commercial fields in Pune, western Maharashtra, India, exhibited chlorotic spots, veinal chlorosis, mosaic, blister formation and shoestring symptoms on leaves, stunted growth, and distortion of fruits. Incidence of virus infection in the fields varied from 25 to 38%. Crude sap extracted from infected cucumber leaf samples was inoculated mechanically onto cucumber and indicator host plants. The inoculated glasshouse-grown cucumber plants showed virus symptoms similar to those observed in the field. The virus produced

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von Bargen, S., T. Büttner, H. P. Mühlbach, J. Robel, and C. Büttner. "First Report of European mountain ash ringspot-associated virus in Sorbus aucuparia in Norway." Plant Disease 98, no. 5 (2014): 700. http://dx.doi.org/10.1094/pdis-09-13-0955-pdn.

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In July 2012, leaf mottle and intensive chlorotic ringspots were observed on urban, forest, or roadside mountain ash trees (Sorbus aucuparia L., rowan) of different ages in Norway during visual inspection of native broadleaf forest tree species. Symptoms resembled those caused by European mountain ash ringspot-associated virus (EMARaV), the type-member of the newly established genus Emaravirus, containing segmented ss(-)RNA and infecting woody host species (2). Leaves of nine out of 30 assessed rowan trees exhibiting characteristic symptoms were sampled in the counties of Nordland and Nord-Trø

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Nischwitz, C., A. L. Maas, S. W. Mullis, A. K. Culbreath, and R. D. Gitaitis. "First Report of Peanut mottle virus in Forage Peanut (Arachis glabrata) in North America." Plant Disease 91, no. 5 (2007): 632. http://dx.doi.org/10.1094/pdis-91-5-0632a.

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Rhizoma peanut (Arachis glabrata Benth.) is a forage crop with increasing acreage (>10,500 ha) in the coastal plain region of the United States. Peanut mottle virus (PeMoV), a member of the family Potyviridae, is transmitted nonpersistently by aphids and seed-transmitted in A. hypogaea. Important hosts of the virus include peanut, soybean, and pea. During January of 2006 in Tifton, GA, immature rhizoma peanut plants identifier A176 with a lost PI number and PI 243334 exhibiting chlorotic ringspots were tested for viruses (potyviruses, Tomato spotted wilt virus [TSWV] and Cucumber mosaic vir

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Liu, H. Y., and J. L. Sears. "First Report of Pelargonium zonate spot virus from Tomato in the United States." Plant Disease 91, no. 5 (2007): 633. http://dx.doi.org/10.1094/pdis-91-5-0633b.

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Pelargonium zonate spot virus (PZSV) was first isolated from tomato in southern Italy in 1982 (1) and later was also reported from Spain (3) and France (2). Infected tomato plants showed stunting, malformation, yellow rings and line patterns on the leaves, and concentric chlorotic ringspots on the stems. In June of 2006, more than 100 tomato (Lycopersicon esculentum Mill.) plants exhibiting symptoms similar to PZSV were observed in seven acres of tomato fields in Yolo County, California. The causal agent was mechanically transmitted to several indicator species. Symptoms on infected plants inc

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Fan, Xudong, Zunping Zhang, Fang Ren, et al. "Development of a Full-Length Infectious cDNA Clone of the Grapevine Berry Inner Necrosis Virus." Plants 9, no. 10 (2020): 1340. http://dx.doi.org/10.3390/plants9101340.

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Grapevine berry inner necrosis virus (GINV) belongs to the genus Trichovirus in the family Betaflexiviridae. The GINV isolate LN_BETA_RS was obtained from a “Beta” grapevine (Vitis riparia × Vitis labrusca) exhibiting chlorotic mottling and ring spot in Xingcheng, Liaoning Province, China. To verify the correlation between GINV and grapevine chlorotic mottling and ring spot disease, we constructed an infectious cDNA clone of GINV isolate LN_BETA_RS using the seamless assembly approach. Applied treatments of agroinfiltration infectious cDNA confirmed systemic GINV infection of the Nicotianaocci

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Dardick, Christopher. "Comparative Expression Profiling of Nicotiana benthamiana Leaves Systemically Infected with Three Fruit Tree Viruses." Molecular Plant-Microbe Interactions® 20, no. 8 (2007): 1004–17. http://dx.doi.org/10.1094/mpmi-20-8-1004.

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Plant viruses cause a wide array of disease symptoms and cytopathic effects. Although some of these changes are virus specific, many appear to be common even among diverse viruses. Currently, little is known about the underlying molecular determinants. To identify gene expression changes that are concomitant with virus symptoms, we performed comparative expression profiling experiments on Nicotiana benthamiana leaves infected with one of three different fruit tree viruses that produce distinct symptoms: Plum pox potyvirus (PPV; leaf distortion and mosaic), Tomato ringspot nepovirus (ToRSV; tis

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Jones, P., J. Devonshire, A. Dabek, and C. Howells. "First Report of Hibiscus Chlorotic Ringspot Carmovirus in Tuvalu." Plant Disease 82, no. 5 (1998): 591. http://dx.doi.org/10.1094/pdis.1998.82.5.591c.

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In September 1997, plants of Hibiscus manihot (locally called nambele) were observed on Vaitupu Island, Tuvalu, exhibiting an angular leaf mosaic and chlorosis that was not always clearly discernible. Electron microscopy of negatively stained sap from affected leaves revealed the presence of numerous isometric virus particles 28 nm in diameter. Poly-acrylamide gel electrophoresis of purified virus gave a single protein band of Mr 38,000 similar to that of the carmoviruses. Immunosorbent electron microscopy tests with antisera kindly provided by N. Spence showed the virus to be hibiscus chlorot

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Doan, Danny N. P., Kian Chung Lee, Pasi Laurinmäki, Sarah Butcher, Sek-Man Wong, and Terje Dokland. "Three-dimensional reconstruction of hibiscus chlorotic ringspot virus." Journal of Structural Biology 144, no. 3 (2003): 253–61. http://dx.doi.org/10.1016/j.jsb.2003.10.001.

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Robertson, N. L., K. L. Brown, L. M. Winton, and P. S. Holloway. "First Report of Tobacco rattle virus in Peony in Alaska." Plant Disease 93, no. 6 (2009): 675. http://dx.doi.org/10.1094/pdis-93-6-0675b.

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Peonies (Paeonia sp.) are highly valued for their large showy flowers in home gardens and commercially in the cut flower industry. In 2007, scattered peony (Paeonia lactiflora ‘Sarah Bernhardt’) plants cultivated on small plots at the University of Alaska Experimental Station in Fairbanks displayed distinct leaf ringspot patterns. Symptoms were more severe during the cooler months of the growing season (June and September), with symptom remission in the intervening warmer months. Leaf samples from six symptomatic plants were collected in July and from 20 symptomatic plants in September and ass

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