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Dissertations / Theses on the topic 'CHO HOST CELL PROTEINS'

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Published: 11 September 2023

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1

Goey, Cher Hui. "Cascading effects in bioprocessing : the impact of cell culture environment on CHO cell behaviour and host cell protein species." Thesis, Imperial College London, 2016. http://hdl.handle.net/10044/1/52703.

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One of the reasons for the rejection of new drugs during clinical trials is the presence of host cell proteins (HCPs) in the drug formulation. HCPs are immunogenic impurities that can compromise patient safety. Moreover, proteolytic and binding HCPs compromise the integrity, and, hence, the stability and efficacy of a recombinant protein. Therefore, HCPs should be removed from the bioprocess train as soon as possible. Current downstream purification platforms are challenged by HCP-mAb co-elution. A Quality by Design strategy to overcome this problem is to reduce the number of HCPs in the downs

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2

Carr, Sharon. "Adenovirus and its interaction with host cell proteins /." St Andrews, 2007. http://hdl.handle.net/10023/219.

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3

Qashqari, Fadi Saleh I. "Regulation of host cell proteins by adenovirus oncoproteins." Thesis, University of Birmingham, 2017. http://etheses.bham.ac.uk//id/eprint/8020/.

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Adenovirus early region proteins, ElA, E1B-55K, E4orf3 and E4orf6 regulate host cell processes to facilitate viral replication. E4orf3 suppress host cell anti-viral activities through association with host cell proteins in E4orf3 nuclear-track structures, whilst E1B-55K, E4orf3 and E4orf6 are all recruited to viral replication centres during infection to promote viral DNA replication and inhibit host cell antiviral activities. Immunoprecipitation coupled to mass spectrometry identified Toplla as an Ad12 E4orf3-binding protein that localized with E4orf3 in adenovirus-infected cells. It was dete

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4

Bjertsjö, Rennermalm Anna. "Staphylococcal cell wall associated proteins : characteristics and host interactions /." Stockholm, 2005. http://diss.kib.ki.se/2005/91-7140-542-9/.

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5

Huang, Edwin P. C. Biotechnology &amp Biomolecular Sciences Faculty of Science UNSW. "Recombinant protein production utilising a metallothionein expression system and a Super-CHO cell line." Awarded by:University of New South Wales. School of Biotechnology and Biomolecular Sciences, 2006. http://handle.unsw.edu.au/1959.4/24940.

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A novel metal-inducible and amplifiable metallothionein (MT) expression system, pNK, was firstly optimised and characterised for the production of a reporter protein, human growth hormone (hGH) in a suspension CHO cell line grown in a serum-free media. The pNK-based hGH production was demonstrated in cadmium-free condition under various fermentation modes (batch, fed-batch and perfusion) and scales (flask to bench-top bioreactor). Improvement of specific productivity of recombinant protein from pNK was shown to be possible by addition of butyrate or substrate substitution of glutamine by gluta

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6

Mohamed, Ahmed Attia Ali. "Interaction of hepatitis C virus polymerase with host cell proteins." Thesis, Durham University, 2009. http://etheses.dur.ac.uk/2107/.

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Hepatitis C virus (HCV) interacts with host cell proteins to modify cellular pathways creating a favourable environment that facilitates its replication and persistence. The purpose of the work presented in this thesis was to identify cellular proteins that can interact with NS5B, the virus's RNA-dependent RNA polymerase, that may contribute to the virus's biology. A number of cellular proteins were found to interact with NS5B using the yeast two-hybrid system. These proteins were involved in cellular pathways such as interferon signalling, lipid transport and metabolism, protein trafficking,

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7

Goldman, Merlin Hesper. "The effect of process variables on the glycosylation of gamma-interferon produced in CHO cells." Thesis, University College London (University of London), 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.244743.

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8

Patel, Amit. "Interaction of enteropathogenic 'Escherichia coli' (EPEC) tir with host cell proteins." Thesis, University of Nottingham, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.431869.

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9

Cowlishaw, Deborah Anne. "Identification of host proteins required for bacteriophage infection of Streptomyces sp." Thesis, University of Nottingham, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.367589.

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10

Rytkönen, Anne. "Molecular studies of Neisseria - host cell interactions /." Stockholm, 2004. http://diss.kib.ki.se/2004/91-7140-018-4/.

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11

Porter, Alison J. "Analysis of the efficiency of selecting GS-CHO cell lines for cGMP manufacture of recombinant proteins." Thesis, University of Manchester, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.692544.

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The market size for biotherapeutics is growing rapidly and the hunt is always on for the next blockbuster drug. A wide range of cell phenotypes are obtained during the process of generating mammalian cell lines suitable for the production of a biotherapeutic such as a monoclonal antibody. A strategy is therefore required for the selection of a cell line with ’desirable’ characteristics, from a population of closely related cell lines, for the successful manufacture of the biotherapeutic. As this process is included in the sequence of activities which determines the minimum time to start cGMP m

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12

Dickey, Laura Leigh. "Respiratory synctial virus interactions with host-cell RNA-processing structures and proteins." Thesis, Boston University, 2013. https://hdl.handle.net/2144/10980.

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Thesis (Ph.D.)--Boston University<br>Respiratory syncytial virus (RSV) is a negative-strand RNA virus that causes significant pneumonia-related morbidity and mortality worldwide. There are currently neither vaccines nor effective therapies for RSV. As with other viruses, RSV mRNAs are translated using host-cell machinery, rendering the virus subject to cellular factors that regulate mRNA homeostasis. Stress granules (SGs) and processing bodies (p-bodies) are inter-dependent, stress-response cytoplasmic structures involved in mRNA triage and degradation, respectively. We hypothesized that RSV h

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13

Riaz, Muhammad Suleman. "Investigating the effects of host factors (proteins and non-proteins) on mycobacteria." Thesis, Brunel University, 2018. http://bura.brunel.ac.uk/handle/2438/16060.

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Mycobacterium tuberculosis (M.tb), the causative agent of tuberculosis, is one of the leading causes of death due to a single infectious agent and results in more than 1 million human deaths every year. M.tb infection of the host initiates a local inflammatory response, resulting in the migration of a number of host plasma protein and non-protein factors to the site of infection. In addition, some of these factors are also produced locally at the site of infection. It is envisaged that these host factors are likely to come in direct contact with M.tb and immune cells and may modulate the outco

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14

Ratanji, Kirsty. "Investigating the immunogenicity of therapeutic proteins : protein aggregation and host cell protein impurities." Thesis, University of Manchester, 2017. https://www.research.manchester.ac.uk/portal/en/theses/investigating-the-immunogenicity-of-therapeutic-proteins-protein-aggregation-and-host-cell-protein-impurities(fda43dd8-2c1e-492b-abd9-e010774d2219).html.

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The development of anti-drug antibodies (ADA) against therapeutic proteins can impact upon drug safety and efficacy. This is a major challenge in the development of biotherapeutics. Various factors have the potential to contribute to protein immunogenicity and the production of ADA. Protein aggregation is one of these factors, though the mechanisms underlying aggregate immunogenicity are poorly understood. In this thesis the effect of protein aggregation on immunogenicity has been investigated. The thermal and/or mechanical stresses required in order to achieve subvisible aggregates of three t

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15

Ohmer, Michaela [Verfasser], and Georg [Akademischer Betreuer] Häcker. "Regulation of cell survival by host and viral anti-apoptotic Bcl-2 proteins." Freiburg : Universität, 2016. http://d-nb.info/1152210300/34.

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16

Schoggins, John Wesley. "Adenovirus host-cell interactions : the role of capsid proteins in transduction, immune activation, and gene targeting /." Access full-text from WCMC:, 2007. http://proquest.umi.com/pqdweb?did=1432771281&sid=1&Fmt=2&clientId=8424&RQT=309&VName=PQD.

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17

Khairalla, Ahmed Samir Mohammed. "Identification of host immune cell receptors mediating the binding of meningococcal App/MspA proteins." Thesis, University of Nottingham, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.575478.

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Neisseria meningitidis, a major cause of bacterial meningitis and septicemia, secretes multiple virulence factors to the extracellular environment, including the adhesion and penetration protein (App) and the meningococcal serine protease A (MspA). Both proteins belong to the serine protease auto transporter family and have the capacity to adhere to host epithelial and endothelial cells. Besides, both proteins have been previously shown to be internalized and translocated to the nuclei of human brain microvascular endothelial cells and dendritic cells (DCs), where they modulate gene expression

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18

Sokolova, Nadiia Verfasser], and Theresia [Akademischer Betreuer] [Stradal. "Identification and characterization of interactions between bacterial WxxxE-virulence proteins and host cell proteins / Nadiia Sokolova ; Betreuer: Theresia Stradal." Braunschweig : Technische Universität Braunschweig, 2017. http://d-nb.info/1175817961/34.

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19

He, Yupeng. "Modulation of the host cell signaling pathways and protein synthesis by hepatitis C virus nonstructural 5A protein /." Thesis, Connect to this title online; UW restricted, 2002. http://hdl.handle.net/1773/11491.

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20

Curra, Chiara. "Protein trafficking and host cell remodeling in malaria parasite infection." Thesis, Montpellier 2, 2010. http://www.theses.fr/2010MON20219.

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Pour assurer ses besoins de croissance, multiplication, et survie, Plasmodium modifie sa cellule hôte, l'érythrocyte, après l'invasion. Le parasite met en place ainsi un système d'échanges (import/export) avec sa cellule hôte et le milieu extérieur. Nous avons identifié dans la base de données de Plasmodium berghei, le parasite de rongeurs, une famille de gènes, sep, correspondant à la famille etramp chez Plasmodium falciparum. Cette famille de gènes code pour des petites protéines exportées, et conservées dans tout le genre Plasmodium. Les protéines SEP (13?16 kDa) contiennent en N-terminal u

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21

Courtney, Sean C. "Functional Analysis of Host Cell Proteins and Stress Responses that Inhibit West Nile Virus Infection." Digital Archive @ GSU, 2011. http://digitalarchive.gsu.edu/biology_diss/101.

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Resistance to flavivirus-induced disease is conferred by a single gene that encodes oligoadenylate synthetase (Oas) 1b (Oas1b). Oas1b is not a functional synthetase suggesting its anti-flavivirus mechanism is RNase L-independent and that it may be mediated by interactions with other host cell protein(s). A yeast two-hybrid screen was used to identify host cell binding partners of Oas1b. Candidate partners were confirmed by yeast co-transformation and co-immunoprecipitation analyses. Oxysterol binding protein-related 1L (ORP1L) and ATP binding cassette subfamily F 3 (ABCF3) were found to intera

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22

Westlund, Alexander. "Increased expression of therapeutic proteins by identification of 3'-UTRs from high expressing genes in CHO cells." Thesis, Linköpings universitet, Institutionen för fysik, kemi och biologi, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-157593.

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Therapeutic proteins, a.k.a. biopharmaceuticals, are most commonly produced in expression systems derived from Chinese Hamstery Ovary (CHO) cells, thanks to great capacity of post-translational modifications like secretation, folding and glycosylation. The engineering of cells for regulation of protein expression has many options including knock-in and knock-out of genes, epigenetic studies or improvement of the expression casette of the protein of interest by e.g. promotor variants or modifications of the 5’ and 3’ untranslated region (UTR). The 3’-UTR is therefore a good optimization candida

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23

Garcia, Lobato Tavares Raquel. "Host cell responses to Helicobacter pylori secreted factors." Doctoral thesis, Stockholms universitet, Institutionen för molekylär biovetenskap, Wenner-Grens institut, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-148427.

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The infection of the human gastric mucosa by the bacterium Helicobacter pylori can lead to the development of gastritis, gastroduodenal ulcers, and cancer. The factors that determine disease development in a small percentage of infected individuals are still not fully understood. In this thesis, we aimed to identify and functionally characterize novel virulence factors of H. pylori and to understand their effect on host cell responses. In Paper I, we found that JHP0290, an uncharacterized secreted protein of H. pylori, induced macrophage apoptosis concomitant to the release of pro-inflammatory

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24

Suppiah, Suganthi. "Investigation of Interactions of the Rubella Virus P150 Replicase Protein with Host Cell Proteins in Infected Cells." Digital Archive @ GSU, 2009. http://digitalarchive.gsu.edu/biology_diss/86.

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Due to their simplicity, viruses require the assistance of host factors for various aspects of their replication cycle. This study investigated the interaction of one of the two non-structural replicase proteins of rubella virus (RUBV), P150, with cell proteins. RUBV forms replication complexes for replicating its RNA in association with membranes of endosomes and lysosomes; the thusly modified endosomes/lysosomes are termed cytopathic vacuoles or CPVs. In the first study, a RUBV expressing a FLAG epitope-tagged P150 was used to co-immunoprecipitate putative interacting cell proteins from an i

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25

Fischer, Joshua Richard. "Mechanisms of Host Cell Attachment by the Lyme Disease Spirochete: A Dissertation." eScholarship@UMMS, 2005. http://escholarship.umassmed.edu/gsbs_diss/194.

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Host cell binding is an essential step in colonization by many bacterial pathogens, and the Lyme disease agent, Borrelia burgdorferi, which colonizes multiple tissues, is capable of attachment to diverse cell types. Glycosaminoglycans (GAGs) are ubiquitously expressed on mammalian cells and are recognized by multiple B. burgdorferi surface proteins. We previously showed that B. burgdorferi strains differ in the particular spectrum of GAGs that they recognize, leading to differences in the cultured mammalian cell types that they efficiently bind. The molecular basis of these binding specificiti

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26

Vatter, Heather. "Analysis of Simian Hemorragic Fever Virus Proteins and the Host Cell Responses of Disease Resistant and Susceptible Primates." Digital Archive @ GSU, 2013. http://digitalarchive.gsu.edu/biology_diss/128.

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African monkey species are natural hosts of simian hemorrhagic fever virus (SHFV) and develop persistent, asymptomatic infections. SHFV was previously shown to also cause a rapid onset fatal hemorrhagic fever disease in macaques. Infection of macaques with a new isolate of SHFV from persistently infected baboon sera, that showed high nucleotide identity with the lab strain LVR, resulted in viremia, pro-inflammatory cytokine and tissue factor production, and symptoms of coagulation defects. Primary macrophages and myeloid dendritic cell cultures from disease-susceptible macaques efficiently rep

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27

Jassim, Amir. "Involvement of the matrix proteins SPARC and osteopontin in the dynamic interaction between tumour and host cells." Thesis, Brunel University, 2016. http://bura.brunel.ac.uk/handle/2438/13399.

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Osteoblasts are highly active cells that are responsible for secreting bone forming components such as collagen type I and matricellular proteins that mediate collagen deposition and mineralisation. SPARC and osteopontin are matricellular proteins that are involved in bone regulation and cell-matrix interactions and are also upregulated in metastatic disease. Secretion of these proteins results in changes to the stromal environment that includes cell migration, angiogenesis, matrix degradation, matrix deposition, bone mineralisation and bone resorption. Signalling pathways not only lead to the

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28

Chang, Po-Hsun. "Characterization of the Outer Membrane of Treponema Pallidum Subsp. Pallidum by Binding Studies Using Antibodies, Complement, and Host Serum Proteins." Thesis, University of North Texas, 1989. https://digital.library.unt.edu/ark:/67531/metadc798468/.

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The major goal of this study was to achieve sustained cultivation of virulent T. pallidum in vitro. The putatuive binding of host proteins to the outer membrane (OM) of intact, virulent T. pallidum subsp. pallidum has been investigated. A major breakthrough was the development of a filtration assay, usinglow protein-binding membrane filters, for the measurement of substances bound to or incorporated into th eOM of T. pallidum. This avoided the conventional manipulations which can damage the fragile OM of T. pallidum. Using this filtration assay, studies on the binding of host serum proteins de

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29

Rhein, Bethany Ann. "Ebola virus: entry, pathogenesis and identification of host antiviral activities." Diss., University of Iowa, 2015. https://ir.uiowa.edu/etd/6629.

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Ebola virus (EBOV) is a member of the Filoviridae family of highly pathogenic viruses that cause severe hemorrhagic fever and is the causative agent of the 2014 West Africa outbreak. Currently, there are no approved filovirus vaccines or treatments to combat these sporadic and deadly epidemics. One target for EBOV antiviral therapy is to block viral entry into host cells. Recently, phosphatidylserine (PtdSer) receptors, primarily known for their involvement in the clearance of dying cells, were shown to mediate entry of enveloped viruses including filoviruses. The PtdSer receptors, T-cell immu

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30

Arfi, Zulfaquar Ahmad Verfasser], Rainer [Akademischer Betreuer] [Fischer, and Stefan [Akademischer Betreuer] Schillberg. "Development of an immunoassay to detect tobacco host cell proteins during biopharmaceutical development / Zulfaquar Ahmad Arfi ; Rainer Fischer, Stefan Schillberg." Aachen : Universitätsbibliothek der RWTH Aachen, 2015. http://d-nb.info/1129176789/34.

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31

Arfi, Zulfaquar Ahmad [Verfasser], Rainer [Akademischer Betreuer] Fischer, and Stefan [Akademischer Betreuer] Schillberg. "Development of an immunoassay to detect tobacco host cell proteins during biopharmaceutical development / Zulfaquar Ahmad Arfi ; Rainer Fischer, Stefan Schillberg." Aachen : Universitätsbibliothek der RWTH Aachen, 2015. http://d-nb.info/1129176789/34.

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32

Thay, Bernard. "Vesicle-mediated and free soluble delivery of bacterial effector proteins by oral and systemic pathogens." Doctoral thesis, Umeå universitet, Institutionen för odontologi, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-82782.

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Periodontitis, the primary cause of tooth-loss worldwide, is a bacterially induced chronic inflammatory disease of the periodontium. It is associated with systemic conditions such as cardiovascular disease (CVD). However, pathogenic mechanisms of periodontitis-associated bacteria that may contribute to the CVD association are unclear. The aim of this doctoral thesis project was to characterize bacterial mechanisms that can originate from the periodontal pocket and expose the host to multiple effector proteins, thereby potentially contributing to periodontal tissue degradation and systemic stim

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33

Forsman, Alma. "The Epstein-Barr virus nuclear antigens 1 & 5 : study of virus-host cellular protein interactions /." Göteborg : Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine, 2009. http://hdl.handle.net/2077/21357.

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34

Lövkvist, Lena. "Receptor interactions between pathogenic bacteria and host cells /." Uppsala : Acta Universitatis Upsaliensis : Uppsala universitetsbibliotek [distributör], 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7782.

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35

Kanatani, Sachie. "Host-parasite interactions in the dissemination of Toxoplasma gondii." Doctoral thesis, Stockholms universitet, Institutionen för molekylär biovetenskap, Wenner-Grens institut, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-148573.

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Toxoplasma gondii is an obligate intracellular parasite that infects virtually all warm-blooded organisms. Systemic dissemination of T. gondii in the organism can cause life-threatening infection that manifests as Toxoplasma encephalitis in immune-compromised patients. In addition, mounting evidence from epidemiological studies indicates a link between chronic Toxoplasma infection and mental disorders. To better understand the pathogenesis of toxoplasmosis, basic knowledge on the host-parasite interactions and the dissemination mechanisms are essential. Previous findings have established that,

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36

Miller, Cathy Lea. "Investigation of the role of minute virus of mice (MVM) small non-structural protein NS2 interactions with host cell proteins during MVM infection." free to MU campus, to others for purchase, 2001. http://wwwlib.umi.com/cr/mo/fullcit?p3025638.

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37

Huang, Bernice. "Anaplasma phagocytophilum remodels its host cell-derived vacuole into a protective niche by redecorating the vacuolar membrane with select Rab GTPases and bacterial proteins." VCU Scholars Compass, 2011. http://scholarscompass.vcu.edu/etd/280.

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Anaplasma phagocytophilum is an obligate intracellular bacterium that infects neutrophils to cause the emerging tick-transmitted disease, human granulocytic anaplasmosis (HGA). Following entry, the pathogen replicates within a host cell-derived vacuole that fails to mature along the endocytic pathway, does not acidify, and does not fuse with lysosomes. Selective fusogenicity is prototypical of many vacuole-adapted pathogens and has been attributed, at least in part, to pathogen modification of the vacuolar inclusion membrane and/or to selective recruitment or exclusion of host trafficking regu

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Eccleston, Ruth Charlotte. "A mechanistic model predicting cell surface presentation of peptides by MHC class I proteins, considering peptide competition, viral intracellular kinetics and host genotype factors." Thesis, University College London (University of London), 2017. http://discovery.ucl.ac.uk/10038760/.

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Major histocompatability complex class I (MHC-I) proteins present short fragments of pathogenic or cancerous proteins (peptides) on the surface of infected cells for recognition by T lymphocytes which are stimulated upon recognition of foreign peptides. Due to the diversity of peptide sequences and the sequence-specificity of MHC-I alleles, being able to determine which peptides will be presented by which MHC-I alleles and in what proportion could be important for the development of vaccines and treatments based on the presented peptiodome. Machine learning tools, trained on experimental data,

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Husson, Gauthier. "Development of host cell protein impurities quantification methods by mass spectrometry to control the quality of biopharmaceuticals." Thesis, Strasbourg, 2017. http://www.theses.fr/2017STRAF066/document.

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Les récents progrès instrumentaux en spectrométrie de masse, notamment en terme de- rapidité de balayage et de résolution, ont permis l'émergence de l'approche « data independent acquisition» (DIA). Cette approche promet de combiner les points forts des approches « shotgun » et ciblées,mais aujourd'hui l'analyse des données DIA reste compliquée. L'objectif de cette thèse a été de développer des méthodes innovantes de spectrométrie de masse, et en particulier d'améliorer l'analyse des données DIA. De plus, nous avons développé une approche originale Top 3-ID-DIA, permettant à la fois un profila

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Ebrahimi, Majid. "Studies of p63 and p63 related proteins in patients diagnosed with oral lichen planus." Doctoral thesis, Umeå : Univ, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1437.

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Ahlqvist, Jenny. "Differences in tropism and viral assembly pathways of human herpesvirus 6A and 6B (HHV-6A and 6B) and association of host cell proteins in HHV-6A virions /." Stockholm, 2007. http://diss.kib.ki.se/2007/978-91-7357-214-9/.

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Thorsén, Jenny. "Purification of His-tagged Proteins Using WorkBeads 40 TREN as a Pre-Treatment Step Prior Loading Sample onto IMAC Resins with the Purpose to Enhance Performance." Thesis, Uppsala universitet, Biokemi, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-439642.

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This work is the result of evaluating a novel strategy for the purification of recombinant His-tagged proteins. Proteins purified in this study were the E. coli translational proteins IF-3, RF-1, and RFF. The study aimed to analyse the potential of using Bio-Works WorkBeads™40 TREN, a multimodal anion ion exchange chromatography resin, as a pretreatment step upstream an immobilized metal ion chromatography (IMAC) resin to enhance performance efficiency of His-tagged protein purification. The method demonstrated here shows potential for anyone seeking to increase the purity of His-tagged protei

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43

Bevington, Joyce M. "Cellular Response to Adenovirus and Adeno- Associated Virus Coinfection." Connect to full text in OhioLINK ETD Center, 2009. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=mco1242921394.

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Dissertation (Ph.D.)--University of Toledo, 2009.<br>"In partial fulfillment of the requirements for the degree of Doctor of Philosophy in Biomedical Sciences." Title from title page of PDF document. Bibliography: p. 70-80, p. 28-158.

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Jonscher, Ernst Georg Wolfgang [Verfasser]. "Identification of proteins involved in host cell cytosol uptake in the human Malaria parasite Plasmodium falciparum : Identifizierung von Proteinen der Wirtszellzytosolaufnahme des humanen Malaria-Erregers Plasmodium falciparum / Ernst Georg Wolfgang Jonscher." Hamburg : Staats- und Universitätsbibliothek Hamburg Carl von Ossietzky, 2018. http://d-nb.info/1223620964/34.

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Danielsson, Niemi Liza. "Host ligands and oral bacterial adhesion studies on phosphorylated polypeptides and gp-340 in saliva and milk /." Doctoral thesis, Umeå : Umeå university, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-32894.

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46

Lassalle, Damien. "Les pore forming toxin chez les Lophotrochozoaires : exemple des organismes Biomphalaria glabrata/ Schistosoma mansoni A New Assessment of Thioester-Containing Proteins Diversity of the Freshwater Snail Biomphalaria glabrata Cholesterol-TEG addition at the 5’end of siRNA allows significant increase of its uptake by hemocytes from Biomphalaria glabrata, the schistosomiasis vector snail. Under review in PeeJ. Schistosoma mansoni lysine specific demethylase (SmLSD1) is a druggable target involved in parasite survival, oviposition and stem cell proliferation." Thesis, Perpignan, 2020. https://theses-public.univ-perp.fr/2020PERP0036.pdf.

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La bilharziose est une maladie touchant 230 millions de personnes dans le monde (source OMS). Cette parasitose est provoquée par le schistosome, un vers plat parasite nommé Schistosoma mansoni. Avant de pénétrer dans l’organisme humain par la peau, ce parasite se développe chez un escargot d’eau douce, Biomphalaria glabrata, qui lui sert d’hôte intermédiaire. Nous avons dans ce contexte identifié et étudié deux protéines appartenant à la famille des pore formingtoxins (PFTs), que nous avons appelé Biomphalysine et Glabralysine. Les PFTs sont des effecteurs très connus dans le monde procaryote

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Seung, Edward. "CD40-CD154 Blockade Facilitates Induction of Allogeneic Hematopoietic Chimerism and Transplantation Tolerance: A Dissertation." eScholarship@UMMS, 2003. https://escholarship.umassmed.edu/gsbs_diss/103.

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Allogeneic hematopoietic chimerism leading to central tolerance has significant therapeutic potential. Establishment of hematopoietic chimerism created by stem cell transplantation has been shown to prevent and cure a number of autoimmune diseases and induce the most robust and long-lasting form of transplantation tolerance known. However, the realization of the vast clinical potential of hematopoietic chimerism for induction of transplantation tolerance has been impeded by the toxicity of the host conditioning regimen and the development of graft-versus-host disease (GVHD). This thesis descri

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Tavares, Lucas Alves. "O envolvimento da proteína adaptadora 1 (AP-1) no mecanismo de regulação negativa do receptor CD4 por Nef de HIV-1." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/17/17136/tde-06012017-113215/.

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O Vírus da Imunodeficiência Humana (HIV) é o agente etiológico da Síndrome da Imunodeficiência Adquirida (AIDS). A AIDS é uma doença de distribuição mundial, e estima-se que existam atualmente pelo menos 36,9 milhões de pessoas infectadas com o vírus. Durante o seu ciclo replicativo, o HIV promove diversas alterações na fisiologia da célula hospedeira a fim de promover sua sobrevivência e potencializar a replicação. A rápida progressão da infecção pelo HIV-1 em humanos e em modelos animais está intimamente ligada à função da proteína acessória Nef. Dentre as diversas ações de Nef está a regula

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YADAV, DIVYANSHI. "TO STUDY INTERACTIONS BETWEEN MONOCLONAL ANTIBODY AND CHO HOST CELL PROTEINS TO DESIGN THE WASH PROCESS FOR MAB PURIFICATION." Thesis, 2017. http://dspace.dtu.ac.in:8080/jspui/handle/repository/15879.

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Today, recombinant protein therapies represent a substantial focus of the pharmaceutical industry. Most therapeutic proteins are produced in host cells of non-human origin, including Escherichia coli, yeast, and various mammalian cell lines e.g. Chinese Hamster Ovary Cell Lines. A major focus of all therapeutic protein purification process is to reduce components of host organism, including host cell proteins (HCPs), to levels considered as adequate in the final formulated drug product. HCPs can pose potential safety risks for patients, including immune reactions, decreased product stability,

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Lum, Mabel Yuen Teng. "Identification of host cell proteins involved in Shigella flexneri pathogenesis." Thesis, 2014. http://hdl.handle.net/2440/87369.

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Shigella flexneri is the etiological agent of bacillary dysentery (shigellosis). It is transmitted via the faecal-oral route and is a significant human pathogen due to the high morbidity among children <5 years in developing countries. The key pathogenic features of Shigella include cell death induction in myeloid immune cells and circumventing cell death in colonic epithelial cells, the site of bacterial infection. Shigella also interact with host proteins to initiate de novo actin synthesis to facilitate its intra- and intercellular spread to disseminate in the host. In this thesis, the role

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