Journal articles on the topic 'Cholera Research Laboratory'

Today in Ukraine, nearly all the meat that comes for sale on the agro–industrial markets, produced in farms and private households, where the conditions to receive it, unfortunately, do not guarantee its sanitary quality. Meat rarely get from sick animals are not always fulfilled the requirements for slaughtering and primary processing of carcasses, storage and transportation. Such violations may result in contamination of the slaughter of microorganisms, including salmonella, in turn, contribute to the occurrence of damage processes meat and risks of occurrence of food poisoning, and therefore is a threat to consumers.According to international requirements and the state «the minimum list of research ...» microflora content in meat rationed and microbiological research of meat and meat products are required. But in a state laboratories of veterinary–sanitary expertise in markets, livestock microbiologically investigate.Given the above, we examined the degree of contamination with salmonella carcasses of cattle and pigs that come for sale on the markets of agro farms and private households, salmonella. The study was carried out of the market. Mykolaiv. Research subject carcasses that passed veterinary and sanitary examination in terms of state laboratory of veterinary–sanitary examination of the market, the results of which were accepted for implementation.The results of our studies on pig carcasses 11.1% and 9.1% of carcasses of cattle that came to the market and were made to implement, isolated salmonella Worms for serological and biochemical properties were attributed to serovariantiv S. typhimurium (25.0% ), S. enteritidis (18.7%), S. cholera suis (37.5%) and S. raratyphi (18.7%).Further studies to determine the pathogenic properties of isolated cultures found that 93.7% of them are pathogenic properties inherent in varying degrees, and 18.7% of selected crops (serovarianty S. cholera suis and S. typhimurium) found a high degree of pathogenicity.Investigation of the thermal stability of isolated cultures set high temperature resistant crops serovariantiv S. cholera suis and S. typhimurium – death at a temperature of 90 °C drew after 10 minutes.Our results indicate that under the current system status monitoring safety of meat, there is no preventive aspect, required by international standards, and meat which is sold in the markets can be a source of food poisoning and salmonellosis etiology constitute a danger for the consumer.

Vibrio cholerae is the causative agent of cholera, a notorious diarrheal disease that is typically transmitted via contaminated drinking water. The current pandemic agent, the El Tor biotype, has undergone several genetic changes that include horizontal acquisition of two genomic islands (VSP-I and VSP-II). VSP presence strongly correlates with pandemicity; however, the contribution of these islands to V. cholerae’s life cycle, particularly the 26-kb VSP-II, remains poorly understood. VSP-II-encoded genes are not expressed under standard laboratory conditions, suggesting that their induction requires an unknown signal from the host or environment. One signal that bacteria encounter under both host and environmental conditions is metal limitation. While studying V. cholerae’s zinc-starvation response in vitro, we noticed that a mutant constitutively expressing zinc starvation genes (Δzur) congregates at the bottom of a culture tube when grown in a nutrient-poor medium. Using transposon mutagenesis, we found that flagellar motility, chemotaxis, and VSP-II encoded genes were required for congregation. The VSP-II genes encode an AraC-like transcriptional activator (VerA) and a methyl-accepting chemotaxis protein (AerB). Using RNA-seq and lacZ transcriptional reporters, we show that VerA is a novel Zur target and an activator of the nearby AerB chemoreceptor. AerB interfaces with the chemotaxis system to drive oxygen-dependent congregation and energy taxis. Importantly, this work suggests a functional link between VSP-II, zinc-starved environments, and energy taxis, yielding insights into the role of VSP-II in a metal-limited host or aquatic reservoir.

IntroductionIn August 2017, a cholera outbreak started in Muna Garage Internally Displaced Persons camp, Borno state, Nigeria and >5000 cases occurred in six local government areas. This qualitative study evaluated perspectives about the emergency response to this outbreak.MethodsWe conducted 39 key informant interviews and focus group discussions, and reviewed 21 documents with participants involved with surveillance, water, sanitation, hygiene, case management, oral cholera vaccine (OCV), communications, logistics and coordination. Qualitative data analysis used thematic techniques comprising key words in context, word repetition and key sector terms.ResultsAuthorities were alerted quickly, but outbreak declaration took 12 days due to a 10-day delay waiting for culture confirmation. Outbreak investigation revealed several potential transmission channels, but a leaking latrine around the index cases’ house was not repaired for more than 7 days. Chlorine was initially not accepted by the community due to rumours that it would sterilise women. Key messages were in Hausa, although Kanuri was the primary local language; later this was corrected. Planning would have benefited using exercise drills to identify weaknesses, and inventory sharing to avoid stock outs. The response by the Rural Water Supply and Sanitation Agency was perceived to be slow and an increased risk from a religious festival was not recognised. Case management was provided at treatment centres, but some partners were concerned that their work was not recognised asking, ‘Who gets the glory and the data?’ Nearly one million people received OCV and its distribution benefited from a robust infrastructure for polio vaccination. There was initial anxiety, rumour and reluctance about OCV, attributed by many to lack of formative research prior to vaccine implementation. Coordination was slow initially, but improved with activation of an emergency operations centre (EOC) that enabled implementation of incident management system to coordinate multisectoral activities and meetings held at 16:00 hours daily. The synergy between partners and government improved when each recognised the government’s leadership role.ConclusionDespite a timely alert of the outbreak, delayed laboratory confirmation slowed initial response. Initial responses to the outbreak were not well coordinated but improved with the EOC. Understanding behaviours and community norms through rapid formative research should improve the effectiveness of the emergency response to a cholera outbreak. OCV distribution was efficient and benefited from the polio vaccine infrastructure.

Cockroaches can transmit disease to humans both mechanically and biologically, among others, dysentery, diarrhea, cholera, hepatitis A virus and polio in children. One effort that can be done in reducing the American cockroach without using synthetic insecticides is by using durian leaf extract (DuriozibethinusMurr). The purpose of this study was to determine the content of secondary metabolite compounds present in the skin of durian, determining the mortality of the American cockroaches and determining the LC50 value. This research was conducted by using experimental laboratory method in vivo. Durian leather extracts were made in various concentrations of 5%, 10%, 20%, and 30%, then put the American cockroach into the test box and observed for 3 hours. Based on the research, durian leaf extract contains flavonoid compounds, saponins, tannins and alkaloids. Percentage of mortality of American cockroaches. at concentrations of 5%, 10%, 20% and 30% respectively ie 11.11%, 22.22%, 38.88% and 61.11% with LC50 values ​​of 2,63x105 ppm. It can be concluded that durian skin extract is capable of killing American cockroaches characterized by the percentage of death of American cockroaches.

Water is one of the biggest components from environment and an important aspect of living organism. The source of water that can be used by community to fulfill their needs is river water, but in recent years, the river water is no longer clear. There are various kinds of water pollution/ contamination sources, including waste from community whether it is from household waste or agriculture waste in smaller to bigger scales, or also industrial waste that can cause environment-based diseases such as cholera, dysentery, skin-disease, typhus, and so on that originated from dirty water Furthermore, if the river water has been contaminated with chemicals, especially heavy metals, it can cause severe toxic effects that will lead to death. Therefore, the aim of this research are knowing the quality of river water on Burai Village, Tanjung Baru Subdistrict, Ogan Ilir District, and analyzing the toxic effects that will occur due to the pollution/ contamination. Water examination was conducted in Laboratory of BTKL Palembang and Laboratory of Science and Technology Faculty UIN Raden Fatah Palembang. From the results of examination, it was found that physically the water was colored, smelly, and turbid, also the existance of heavy metals and numbers of high coliform, around >1600/100 mL samples. This is very dangerous because it can contaminate the aquatic biota in the river and the community whose consuming it can lead to disease or even heavy metal poisoning.

The research work was performed for the isolation and identification of Pasteurella multocida from field cases, preparation of oil adjuvanted vaccine from isolated strain and determination of its efficacy. Samples were collected from suspected dead birds of three poultry farms of Bangladesh (Code name: M and R). The P. multocida isolates were Gram negative, non-motile, non- spore forming rod occurring singly or pairs and occasionally as chains or filaments. Biochemically P. multocida ferment basic sugar and consistently produced acid except from maltose and lactose. After isolation formalin killed oil adjuvanted Fowl cholera vaccine was prepared in Laboratory of the Department of Microbiology and Hygiene, BAU and this experimental vaccine (3.2x108 CFU/ml) was administered in nine weeks old White Leg Horn chickens at the different dose rate through intramuscular (IM) route in each selected group A (1ml alum precipitated vaccine), B (0.5ml alum precipitated vaccine), C (1ml oil adjuvanted vaccine) and D (0.5ml oil adjuvanted vaccine). Pre-vaccinated sera were collected from all groups of birds. The mean of Passive Hemagglutination (PHA) titers of post-vaccination were 51±17.8, 76.8±17, 89.6±17, and 115±17.81 in group A, B, C and D respectively which consist of 5 birds in each. The vaccine produced better immune response when boostering with the similar dose and route at 15 days after primary vaccination. The mean PHA titers were higher at group D than other groups after boostering. Challenge infection was conducted on all the vaccinated and control group (n=5) of birds after 15 days of vaccination which protect 93.75% of birds and the PHA titers from different groups analyzed to determine the protective capacity of vaccinated chickens against challenge exposure. It was demonstrated that experimental oil adjuvanted fowl cholera vaccine with 0.5ml dose produce higher immune response against challenge infection and found to be safe. Microbes and Health, June 2013, 2(1): 1-4DOI: http://dx.doi.org/10.3329/mh.v2i1.17253

With a considerably high level of poverty, high population density and relatively fragile health systems, most African countries have a predominance of factors that could contribute to the rapid spread of the COVID-19 pandemic. Despite these challenges, the continent has shown capacity in its response to the pandemic. This may be related to the continent’s experience in responding to several infectious disease outbreaks such as Ebola disease, Lassa fever and cholera. Since the beginning of the COVID-19 pandemic, several local innovations have been developed and implemented. These innovations take into consideration unique circumstances in countries such as multiple government levels, belief in traditional medicine, limited access to medical supplies and others. This paper describes the various strategies developed in African countries across leadership and coordination, surveillance, laboratory capacity, case management, infection, prevention and control, risk communications, points of entry, research, logistics and supply chain, partnership, food security and education. We highlight the impact of these strategies on the response so far, and lessons that other regions across the world can learn from Africa’s response to COVID-19. Finally, we recommend the urgent need for increased investment in African health and social institutions to enable the development of African-owned and led strategies in response to disease outbreaks.

Many genetically modified (GM) crops were used for production of plant-derived edible vaccines and other therapeutic recombinant products. However, GM crops resulted in the ecotoxicological risk of gene transfer because of pollen flow during the past 20 years. The most-commonly used eukaryotic model alga, Chlamydomonas reinhardtii has recently been shown the potential of decreasing this risk. Compared to GM crops, there is no risk of gene transfer because the alga culture can be deserved completely scrutiny under laboratory condition and it do not produce pollen. Recently, we had transformed the chloroplast of Chlamydomonas reinhardtii with two genes, CTB and CV1, which encode cholera toxin B subunit and chimeric antigen CV1 fused CTB with VP1 protein from foot and mouth disease virus (FDMV). The transgenic alga subculture were carried out under different selective conditions. The recombinant antigen in transgenic Chlamydomonas chloroplast was detected by western blotting in a period of subculture time. However, the PCR detection data demonstrated that transgene integrated with chloroplast genome would be lost in a special time when was connected with subculture condition. Although loss of transgenic fragment was an inevitable fate for the green alga, our research data showed the possibility that the presence of transgenic fragment was strictly regulated. Thus, the alga can be used for a safer platform for the production of recombinant product than GM crops.

Background:Uganda is prone to viral hemorrhagic fever (VHF) outbreaks. Infection prevention and control capacity is critical to supporting patient care, to preventing nosocomial transmission to health workers, and to limiting spread within the community. Offsite didactic training may increase healthcare worker knowledge, but this approach may be inadequate for assuring confident execution of practical clinical tasks in patient care settings. We aimed to develop a competency-based, onsite mentorship model for sentinel case isolation and management of viral hemorrhagic fever syndromes in Uganda. Methods: The Naguru Regional Referral Hospital (China Uganda Friendship Hospital) Kampala was selected as a site for training after its designation by the Uganda Ministry of Health (MoH) as facility for isolation of healthcare workers with suspected or confirmed VHF. The need for mentorships was determined from information from training providers, MoH assessments, hospital management, and key hospital staff. A list of skills was developed by reviewing WHO case management guidelines and Uganda-approved VHF trainings. The skills, exercised using scenario-based drills, focused on safety practices, identification and isolation of suspect cases, and delivery of optimized clinical care to suspected cases of VHF, among others. Trained facilitators (n = 2–4) supervised drills attended by staff from Naguru and other Kampala-based health facilities. Drills were scheduled weekly and were ordered to progressively increase in complexity. Specific drills could be repeated at the subsequent mentorship visit if gaps were identified. Results: Over 3 months, 12 drills were completed (Table 1). Cadres trained included 10 medical doctors, 12 nurses, 3 clinical officers, 5 laboratory technicians, 6 hygienists, 2 security officers, and 3 administrative officers. On average, 8 hospital staff attended weekly drills. During 3 months of the intervention, 1 suspected case of VHF and 3 cases with laboratory confirmed cholera were managed by the hospital team, and staff demonstrated the capacity for safe handling of patients with infectious bodily fluids. Barriers encountered included practice fatigue from repeated drills, challenges with team cohesion since members were from different institutions, limited personal protective equipment for repeated trainings, and competing routine hospital activities that reduced numbers of staff available for training. Repeated drills included clinical management, cadaver management, and infectious spills. Conclusions: This onsite mentorship project supported healthcare workers to gain confidence in the management of suspected VHF infection and other highly infectious diseases. Continued mentorship, hospital administration support and increase in exercise complexity are needed to consolidate on these gains.Funding: NoneDisclosures: Mohammed Lamorde reports contract research for Janssen Pharmaceutica, ViiV, and Mylan.

Molecular microbiologists depend heavily on laboratory strains of bacteria, which are ubiquitous across the community of research groups working on a common organism. However, this presumes that strains present in different laboratories are in fact identical. Work on a culture of Vibrio cholerae preserved from 1916 provoked us to consider recent studies, which have used both classical genetics and next-generation sequencing to study the heterogeneity of laboratory strains. Here, we review and discuss mutations and phenotypic variation in supposedlyisogenic reference strains of V. cholerae and Escherichia coli , and we propose that by virtue of the dissemination of laboratory strains across the world, a large ‘community evolution’ experiment is currently ongoing.

This research was conducted in Microbiology Laboratory of Biology Department, Faculty of Mathematics and Natural Sciences, Padjadjaran University in May 2017. The purpose of the research was to identify the number and types of microbes contained in carp (Cyprinus carpio) in Jatiluhur Reservoir, West Java. The method used in this research was sample survey method or field survey. The water and common carp sampling stations are divided into 3 stations, station 1 is located in Zone 1 (low density), station 2 is located in Zone 3 (medium density), and station 3 is located in Zone 5 (high density). Determination of sampling location water and fish is done based on Data Recapitulation of Density of Karamba Floating Net from the Department of Animal Husbandry and Fisheries of West Java.Water samples and fish mucus were tested using Total Plate Count (TPC) method using different specific media that is Trypticase Soy Agar (TSA), deMann Ragosa Sharpe Agar (MRSA), Kliger Iron Agaar (KIA), Thiosulphate Citrate Bile salts Sucrose Agar (TCBS), and Xylose Lysine Deoxycholate (XLD). Microbes can be known by species because they just grown on specific media that can directly showed the specific characteristics of certain microbial species based on the color of the colony. The parameter observed were microbiological test (TPC). The result of the research showed that there was Bacillus sp, Escherichia coli, Lactobacillus sp, Vibrio cholerae, Vibrio parahemoliticus, Vibrio spp, dan Shigella sp on the water samples and carp mucus. The result of research on water samples and fishery products in Jatiluhur Reservoir showed that water quality in Zone 1, Zone 3, and Zone 5 is still below the threshold of microbial contamination based on SNI 7388: 2009, while fish located in Zone 3 and Zone 5 is contaminated by Escherecia coli and Vibrio cholerae, exceed the threshold of SNI 7388: 2009 on microbial contamination in fresh fish.

This study was conducted to determine the microbiological quality of fresh raw and unwashed leafy and salad vegetables in Dhaka, Bangladesh. Six different types of samples (red amaranth, spinach, carrot, radish, tomato, and cucumber) were collected in pre-sterilized zip-lock bags from various local markets in Dhaka. All samples were transported to the Centre of Excellence for Global Health Research Laboratory of Primeasia University at earliest convenience. Samples were enriched in Nutrient Broth media and were then cultured on selective media for isolation purpose. Serial dilution was performed for the total viable count. Biochemical and carbohydrate profiling was conducted for the presumptive identification of the isolates. Commercial antibiotic discs were used for antibiogram by Kirby-Bauer disc diffusion method on Mueller-Hinton agar medium. The total microbial load ranged from 8x107 to 1.70x108. Total 36 isolates were identified, having 7 differentorganisms. The most predominant organism was Vibrio sp. (23%) followed by Klebsiella sp.(20%), Acinetobacter sp. (19%), Pseudomonas sp. (19%), Salmonella sp. (8%), Moraxella sp.(8%) and Escherichia coli (3%). 11% of the Vibrio sp. isolates were V. cholerae, found from 4 samples. No presence of V. cholerae was observed in the tomatosamples. E. coli was observed only in Carrot sample. Antibiotics from 7 different groups were tested against the organisms among which Imipenem showed the highest sensitivity (86%). Following Ceftriaxone (100%), Nitrofurantoin (94%), Erythromycin (89%) and Amoxicillin (83%) had the highest resistance against the isolated organisms. Moreover, most of the isolates showed a multi-drug resistance pattern where they were resistant to at least four drugs. Prevalence of pathogenic bacteria in raw unwashed vegetables can cause potential adverse health effects and therefore the consumers need to be conscious about the matter.

In this field, complex disinfectants were contrasted using effective concentrations against bacteria and spore-forming microorganisms. A significant problem for the owners is the emergence of resistance of strains of microorganisms in the existing production and the same disinfectants. Investigation of trusted working concentrations of disinfectants that do not have a corrosive effect on metal structures. According to the results of research and production, contrast is an effective disinfectant for reducing E. coli and S aureus at 0,3 – 0,5% concentration at exposure for 30 min. and consumption of 100 - 400 cm3 / m2. Laboratory researches were carried out in laboratories of microbiological faculties of veterinary medicine of Sumy National Agrarian University. Disappearance gaps and disinfection on the market in Kyiv. Samples were drawn in the refrigerators from the walls, ceilings and floors. Refrigerated chambers made of stainless steel are made up of rubber and plastic elements. Metals are very vulnerable to corrosion with more concentrated acids and alkali. This was taken into account when choosing a disinfectant and its effective organizations. As a disinfectant used the drug contrast (manufacturer PE "Kronos Agro", Ukraine). The disinfectant was taken at a dose of 100 ml per 1 m2. To produce the culture was prepared basic products containing 1000 mg of the drug in 1 ml of distilled water. The experimental solutions were prepared for study with the main formulations developed. Representatives had extraordinary effects. The disks were leaked through the disinfectant to obtain a zone of retention of high bacteria and fungi. The reliability of disinfectant destroying the micro-organisms of E. coli and S. aureus test cultures was also known. For the trusted production preparations, the contrast at the trusted enterprises was carried out by the rehabilitation of the premises of the educational laboratory of the Sumy National Agrarian University (premises for animals, dairies, refrigeration chambers). Renovation and test activities were performed at 0,1, 0,25 and 0,5 % of exposed contrast with exposure for 60, 30 and 10 min. the disinfectant was consumed 250 cm3 / m2. Upon completion of the studies, the investigated tests were investigated with distilled water (flow rate - 1000 cm3 / m2). Water that has been washed away test products, presented in specially prepared capabilities. Test results for this after disinfection were also investigated with distilled water, which was found on the disinfectant. To study the corrosion activity of the disinfectant used metal plates 1h1sm2. The metal samples were welded to the fifth mark after the commission before and after the study. 0,5; 1,0; 1,5; 2,5 % contrast solutions were used in the study. For comparison, in the experiments used 2% of productive. M. bovis mycobacterial cultures were grown on Pavlovsky's glycerol medium. The bacterial culture was transferred into sterile vials and sterile isotonic material containing 0,05 cm3 / mg was added. A large amount of delay zone in Petri dishes containing 0,5 % of S. aureus disinfectant, 3,5 times S. cholerase 1,8 S. Enteritidis 2 more than 0,5% formal form . Higher indicators of bacterial properties of the disinfectant contrast at a concentration of 0,5%.

Cholera is an acute form of the diarrheal disease that plagued human civilization over the centuries. The enormity of human sufferings led clinicians and scientists to carry out extensive research on cholera and Vibrio cholerae leading to major discoveries that opened up novel areas of research or new disciplines in biomedical sciences. An attempt is made here to summarize some of these breakthroughs and outline their significance in broader perspectives. In the present study, a total of 12 samples were collected from four types of water sources for isolation of Vibrio spp. Water samples are enrichment into alkaline peptone water then inoculate into culture media such as Nutrient agar, MacConkey, and Thiosulfate Citrate Bile Salts Sucrose (TCBS) agar medium. After incubation of TCBS plates for 24h at 37°C yellow and green colonies are screened out for biochemical identification. No-sucrose fermenting Vibrio parahaemolyticus, Vibrio mimicus, and Vibrio vulnificus show green colonies and sucrose fermenting Vibrio cholerae and Vibrio alginolyticus show yellow colonies on TCBS. To identify the Vibrio spp isolates biochemical test was carried out and typical Vibrio spp give a positive result. The majority of Vibrio spp are avirulent, but certain strains may be sporadically human pathogenic. The antibiotic resistance studies showed that among the 9 isolates were resistant against Erythromycin, Penicillin, Cephalexin, Vancomycin and 4 isolates resistant against Tetracycline. To expand current knowledge of the occurrence, ecological niche and persistence of potential human pathogenic Vibrio spp in aquatic environments, occurrence, and laboratory studies were performed.

ObjectiveTo prove the role of partnerships in Disease Surveillance and Response to emerging public health threats in Kerala state, India.IntroductionKerala is a small state in India, having a population of only 34 million (2011 census) but with excellent health indices, human development index and a worthy model of decentralised governance. Integrated Disease Surveillance Program, a centrally supported surveillance program, in place since 2006 and have carved its own niche among the best performing states,in India. Laboratory confirmation of health related events/disease outbreaks is the key to successful and timely containement of such events, which need support from a wide range of Laboratories-from Primary care centers to advanced research laboratories, including private sector. In a resource constraint setting, an effective model of Partnership have helped this state in achieving great heights. Networking with laboratores of Medical Education Department, and Premier Private sector laboratories, Financing equipments and reagents through decentralised governance program, resource sharing with other National programs, Laboraotries of Food Safety, Fisheries and Water authorities have resulted in laboratory confirmation of public health events to the extend of 75-80% in the past 5 years in the state. Etiological confirmation accelerated response measures, often multidsciplinary, involving Human health sector, Animal Health, Agriculture, wild life and even environemntal sectors, all relevant in One Health context.MethodsDuring 2013-14,state launhced a laboratory networking initiative, with aid and guidance from central government,through a mutually beneficial MoU, linking all the 5 Govt Medical College Microbiology Laboratories with the State Health surveillance system. A State Laboratory Cordinator was designated, and these teaching Hospital were requested to assist the state in testing of outbreak samples from adjoining 3-4 districts.Additional funds were provided for these institutions after a team assessment and periodic monitoring.All the 14 disricts of state gained remarkably in laboratory confirmation of various outbreaks.During 2013, when one of the remote districts in the state detected an unusual fever cluster among the indigenous community, investigation by a multidisciplinary team, supported by a reputed private sector virology laboratory of an academic institution of the neighbouring state, confirmed Lyme disease, first time in the state. In 2014 and 2015, the same laboratory confirmed another hitherto unreported disease, Kyasanur Forest disease, in the same district. These two events lead to the establishing of a Private Public Partnership model in disease surveillance in the state. This model shared physical infrastructure in the govt hospital premises with technological support from the virology center. Since then, this laboratory has contributed to >90% of laboratory confirmation of health events in the district. Eventually, the same laboratory became the pioneer in confirmation of the first Nipah Virus outbreak in the state in 2018. This laboratory is also the Reference laboratory for H1N1 and Avian Influenza for whole of South India. This surveillance network, has since then, established additional units in other parts of the state through special government order.From the response perspective also, the state adopted similar partnership approach. The strategy for control of Kyasanur Forest Disease(KFD) is a classical example. Monkey deaths were autopsied by Wildlife experts, domestic animals were treated for tick infestation by the veterinary officers, research work done at Veterinary university, human cases treated and vulnerable population vaccinated by Human Health officers, Tribal and Revenue department addressed the welfare aspects of the affected indigenous communities, and the district collector cordinated all related activities. It was a pathbreaking experience, and since 2015, till date, no new case is reported from the district, unlike hotspots in other parts of India.In 2014, the state gained from Fisheries department laboratory, by confirmation of a fish toxin from an event of food borne infection outbreak. In the same year, Veterinary Univerisity laboratory isolated Vibrio Cholera from water samples from a Cholera outbreak.In 2018, the state surveillance unit, engaged with Veterinary University of the state to undertake MAT testing of Human Leptospirosis cases for facilitating the identification of serovars, another landmark effort, approved by Govt of India. The state surveillance system also receives tremendous support from laboratories of research centers like Rajeev Gandhi center for Biotechnology and Vector Control Research Center of ICMR (Indian council of Medical Research center). The state is now, preparing a draft action plan for constituing a One Health Governance Secretariate in Kerala, to bring together all the stakeholders in disease surveillance, for optimizing their contribution.ResultsState Health surveillance system detected 135,130,140,130,disease outbreaks during the years 2014,15,16,17,and 93, till date in 2018. The laboratory confirmation of 65%,75%,80%,82.5% and 65.5% in respective years facilitated prompt response by the state. This was made possible with an extensive laboratory collaboration with partners ranging from Institutional labs of state government as well as decentralised local self governments,(12.3%) Regional Public Health labs(13.8%), Referral Network Labs of Govt Medical College Hospitals (16.2%), Manipal Center for Viral Research Lab(11.5%) Kerala Water Authority Labs (6.2%), Food Security and Safety department (2.3%) and a small contribution by Private Laboratories (1.5%) during 2017. In 2018, 324 human samples were tested and 16 samples confirmed for Nipah virus disease,from MCVR Manipal. The same laboratory confirmed Lyme disease (2013) and Kyasanur Forest Disease (2014 and 2015) from human sampels. 3 environemntal samples were tested positive for Legionnaires bacteria from cooling system of 2 Tourist Hotels, following notification of Legionnaires Pneumonia among 2 foreign tourists.(2016 and 17). Fish toxin "Ciguaterin" was confrimed from an incident of food borne outbreak by a laboratory attached to Fisheries department ( 2015) - a unique example of One Health application in disease surveillance and outbreak response. Laboratories attached to Kerala Water Authority supports testing of water sampels during water borne infections and Food Safety department facilitates analysis of food items during food borne infections. 7 water samples tested positive for Vibrio Cholerae during a Cholera outbreak, done through Reserch wing of Veterinary Univerisity Micobiology Lab in 2016. An instance of Primary Amoebic Meningoencephalitis was confirmed through a premier private tertiary center laboraotry.Leptospira serovars are being identified through a collaborative project with a Veterinary University(2018).ConclusionsKerala state in India has shown many successful models in development sector. Partnership in Laboratory surveillance is the most recent one in the segment. Besides interdepartmental collaboration, a unique model of Private Public Partnership is also tried by this state, resulting in historic achievements like high eteological confirmation of outbreks including the mos recent and first ever Nipah virus disease,ample evidence for state's commitment to IHR compliance as well.This model, I feel is replicable in similar situations in resource poor countries across the globe.

ABSTRACT Phenotypic diversity between laboratory-domesticated bacterial strains is a common problem and often results in the failed reproduction of published data. However, researchers rarely compare such strains to elucidate the underlying mutation(s). In this study, we tested one of the best-studied V. cholerae isolates, O1 El Tor strain C6706 (a patient isolate from Peru), with respect to two main phenotypes: natural competence for transformation and type VI secretion. We recently demonstrated that the two phenotypes are coregulated and specifically induced upon the growth of pandemic V. cholerae O1 El Tor strains on chitinous surfaces. We provide evidence that of seven C6706 strains collected from different laboratories, four were impaired in the tested phenotypes due to a mutation in a QS gene. Collectively, our data indicate that the circulation of such a mutated wild-type strain of C6706 might have had important consequences for QS-related data. Vibrio cholerae, the causative agent of cholera, is a model organism for studying virulence regulation, biofilm formation, horizontal gene transfer, and the cell-to-cell communication known as quorum sensing (QS). As in any research field, discrepancies between data from diverse laboratories are sometimes observed for V. cholerae. Such discrepancies are often caused by the use of diverse patient or environmental isolates. In this study, we investigated the inability of a few laboratories to reproduce high levels of natural transformation, a mode of horizontal gene transfer that is specifically induced on chitinous surfaces. This irreproducibility was mostly related to one specific isolate of V. cholerae: the O1 El Tor C6706 strain. C6706 was previously described as QS proficient, an important prerequisite for the induction of natural competence for transformation. To elucidate the underlying problem, we collected seven isolates of the same C6706 strain from different research laboratories in North America and Europe and compared their phenotypes. Importantly, we observed a split response with respect to QS-related gene expression, including chitin-induced natural competence and type VI secretion (T6S). While approximately half of the strains behaved as reported for several other O1 El Tor pandemic isolates that are commonly studied in the laboratory, the other half were significantly impaired in QS-related expression patterns. This impairment was caused by a mutation in a QS-related gene (luxO). We conclude that the circulation of such QS-impaired wild-type strains is responsible for masking several important phenotypes of V. cholerae, including natural competence for transformation and T6S. IMPORTANCE Phenotypic diversity between laboratory-domesticated bacterial strains is a common problem and often results in the failed reproduction of published data. However, researchers rarely compare such strains to elucidate the underlying mutation(s). In this study, we tested one of the best-studied V. cholerae isolates, O1 El Tor strain C6706 (a patient isolate from Peru), with respect to two main phenotypes: natural competence for transformation and type VI secretion. We recently demonstrated that the two phenotypes are coregulated and specifically induced upon the growth of pandemic V. cholerae O1 El Tor strains on chitinous surfaces. We provide evidence that of seven C6706 strains collected from different laboratories, four were impaired in the tested phenotypes due to a mutation in a QS gene. Collectively, our data indicate that the circulation of such a mutated wild-type strain of C6706 might have had important consequences for QS-related data.

ABSTRACTVibrio choleraeis an aquatic organism that causes the severe acute diarrheal disease cholera. The ability ofV. choleraeto cause disease is dependent upon the production of two critical virulence determinants, cholera toxin (CT) and the toxin-coregulated pilus (TCP). The expression of the genes that encode for CT and TCP production is under the control of a hierarchical regulatory system called the ToxR regulon, which functions to activate virulence gene expression in response toin vivostimuli. Cyclic dipeptides have been found to be produced by numerous bacteria, yet their biological function remains unknown.V. choleraehas been shown to produce cyclo(Phe-Pro). Previous studies in our laboratory demonstrated that cyclo(Phe-Pro) inhibitedV. choleraevirulence factor production. For this study, we report on the mechanism by which cyclo(Phe-Pro) inhibited virulence factor production. We have demonstrated that exogenous cyclo(Phe-Pro) activated the expression ofleuO, a LysR-family regulator that had not been previously associated withV. choleraevirulence. IncreasedleuOexpression repressedaphAtranscription, which resulted in downregulation of the ToxR regulon and attenuated CT and TCP production. The cyclo(Phe-Pro)-dependent induction ofleuOexpression was found to be dependent upon the virulence regulator ToxR. Cyclo(Phe-Pro) did not affecttoxRtranscription or ToxR protein levels but appeared to enhance the ToxR-dependent transcription ofleuO. These results have identifiedleuOas a new component of the ToxR regulon and demonstrate for the first time that ToxR is capable of downregulating virulence gene expression in response to an environmental cue.IMPORTANCEThe ToxR regulon has been a focus of cholera research for more than three decades. During this time, a model has emerged wherein ToxR functions to activate the expression ofVibrio choleraevirulence factors upon host entry.V. choleraeand other enteric bacteria produce cyclo(Phe-Pro), a cyclic dipeptide that we identified as an inhibitor ofV. choleraevirulence factor production. This finding suggested that cyclo(Phe-Pro) was a negative effector of virulence factor production and represented a molecule that could potentially be exploited for therapeutic development. In this work, we investigated the mechanism by which cyclo(Phe-Pro) inhibited virulence factor production. We found that cyclo(Phe-Pro) signaled through ToxR to activate the expression ofleuO, a new virulence regulator that functioned to repress virulence factor production. Our results have identified a new arm of the ToxR regulon and suggest that ToxR may play a broader role in pathogenesis than previously known.

Objectivea) To observe trends and patterns of diseases of public health importance and responseb) To predict, prevent, detect, control and minimize the harm caused by public health emergenciesc) To develop evidence for managing any future outbreaks, epidemic and pandemicIntroductionDisease surveillance is an integral part of public health system. It is an epidemiological method for monitoring disease patterns and trends. International Health Regulation (IHR) 2005 obligates WHO member countries to develop an effective disease surveillance system. Bangladesh is a signatory to IHR 2005. Institute of Epidemiology, Disease Control and Research (IEDCR <www.iedcr.gov.bd>) is the mandated institute for surveillance and outbreak response on behalf of Government of the People’s Republic of Bangladesh. The IEDCR has a good surveillance system including event-based surveillance system, which proved effective to manage public health emergencies. Routine disease profile is collected by Management Information System (MIS) of Directorate General of Health Services (DGHS). Expanded Program of Immunization (EPI) of DGHS collect surveillance data on EPI-related diseases. Disease Control unit, DGHS is responsible for implementing operational plan of disease surveillance system of IEDCR. The surveillance system maintain strategic collaboration with icddrr,b.MethodsThe IEDCR is conducting disease surveillance in several methods and following several systems. Surveillance data of priority communicable disease are collected by web based integrated disease surveillance. It is based on weekly data received from upazilla (sub-district) health complex on communicable disease marked as priority. They are: acute watery diarrhea, bloody dysentery, malaria, kala-azar, tuberculosis, leprosy, encephalitis, any unknown disease. Government health facilities at upazilla (sub-district) send the data using DHIS2. During outbreak, daily, even hourly reporting is sought from the concerned unit.Moreover, IEDCR conducts disease specific specialized surveillance systems. Data from community as well as from health facilities are collected for Influenza, nipah, dengue, HIV, cholera, cutaneous anthrax, non-communicable diseases, food borne illness. Data from health facilities are collected for antimicrobial resistance, rotavirus and intussusception, reproductive health, child health and mortality, post MDA-surveillance for lymphatic filariasis transmission, molecular xenomonitoring for detection of residual Wucheria bancrofti, dengue (virological), emerging zoonotic disease threats in high-risk interfaces, leptospirosis, acute meningo-encephalitis syndrome (AMES) focused on Japanese encephalitis and nipah, unintentional acute pesticide poisoning among young children. Data for event based surveillance are collected from usual surveillance system as well as from dedicated hotlines (24/7) of IEDCR, media monitoring, and any informal reporting.Case detection is done by syndromic surveillance, laboratory diagnosed surveillance, media surveillance, hotline, cell phone-based surveillance. Dissemination of surveillance is done by website of IEDCR, periodic bulletins, seminar, conference etc. Line listing are done by rapid response teams working in the surveillance sites. Demographic information and short address are listed in the list along with clinical and epidemiological information. Initial cases are confirmed by laboratory test, if required from collaborative laboratory at US CDC (Atlanta). When the epidemiological trend is clear, then subsequent cases are detected by symptoms and rapid tests locally available.ResultsIn 2017, 26 incidents of disease outbreak were investigated by National Rapid Response Team (NRRT) of IEDCR. In the same year, 12 cases of outbreak of unknown disease was investigated by NRRT of IEDCR at different health facilities. Joint surveillance with animal health is being planned for detection and managing zoonotic disease outbreaks, following One Health principles. Department of Livestock, Ministry of Environment and icddrb are partners of the joint surveillance based on One Health principles.Disease Control unit of DGHS, district and upazilla health managers utilizes the disease surveillance data for public health management. They analyze also the surveillance data at their respective level to serve their purpose.ConclusionsA robust surveillance is necessary for assessing the public health situation and prompt notification of public health emergency. The system was introduced at IEDCR mainly for malaria and diarrhea control during establishment of this institute. Eventually the system was developed for communicable disease, and recently for non-communicable diseases. It is effectively used for managing public health emergencies. Notification and detection of public health emergency is mostly possible due to media surveillance.Data for syndromic surveillance for priority communicable diseases is often not sent timely and data quality is often compromised. Tertiary hospitals are yet to participate in the web based integrated disease surveillance system for priority communicable diseases. But they are part of specialized disease surveillances. Data from specialized surveillance with laboratory support is of high quality.Evaluation of the system by conducting research is recommended to improve the system. Specificity and sensitivity of case detection system should also be tested periodically.ReferencesCash, Richard A, Halder, Shantana R, Husain, Mushtuq, Islam, Md Sirajul, Mallick, Fuad H, May, Maria A, Rahman, Mahmudur, Rahman, M Aminur. Reducing the health effect of natural hazards in Bangladesh. Lancet, The, 2013, Volume 382, Issue 9910IEDCR. At the frontline of public health. updated 2013. www.iedcr.gov.bdAo TT, Rahman M et al. Low-Cost National Media-Based Surveillance System for Public Health Events, Bangladesh. Emerging Infectious Diseases. Vol 22, No 4. 2016.<www.iedcr.gov.bd> accessed on 1 Oct 2018.