Academic literature on the topic 'Ciprofloxacin Erythromycin Electrospray ionization mass spectrometry'

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Journal articles on the topic "Ciprofloxacin Erythromycin Electrospray ionization mass spectrometry"

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Jo, Mi-Ra, Jong-Soo Mok, Doo-Seog Lee, Min-Jung Kim, and Poong-Ho Kim. "Determination of Residual Erythromycin Antibiotic in Fishery Products by Liquid Chromatography-electrospray Ionization Mass Spectrometry." Korean Journal of Fisheries and Aquatic Sciences 42, no. 1 (February 28, 2009): 15–19. http://dx.doi.org/10.5657/kfas.2009.42.1.015.

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Wang, Bo, Soyeon Nam, Eunyeong Kim, Hayoung Jeon, Kiho Lee, and Kaizhou Xie. "Identification of Erythromycin and Clarithromycin Metabolites Formed in Chicken Liver Microsomes Using Liquid Chromatography–High-Resolution Mass Spectrometry." Foods 10, no. 7 (June 29, 2021): 1504. http://dx.doi.org/10.3390/foods10071504.

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Nontargeted analysis can be used for the rapid screening and confirmatory analysis of veterinary drugs and their metabolites, which are important for the comprehensive safety evaluation of animal-derived foods. Here, a novel nontargeted screening approach based on liquid chromatography coupled with electrospray ionization–high-resolution mass spectrometry (LC/ESI–HR-MS) was developed to determine erythromycin, clarithromycin, and their metabolites in chicken liver microsomes. Erythromycin and clarithromycin were incubated in vitro in the presence of NADPH for 60 min to generate metabolites in chicken liver microsomes. After the incubation, the supernatant was extracted using ultrasonic shaking, orbital shaking, and centrifugation before analysis using LC/ESI-HR-MS in positive ion mode on an Agilent Eclipse Plus C18 column (100 mm × 2.1 mm; i.d. 3.5 µm) with 0.1 percent formic acid-water and acetonitrile as the mobile phases for gradient elution at 0.4 mL/min. The results show that erythromycin can produce N-desmethyl-erythromycin A in chicken liver microsomes, but clarithromycin cannot produce N-desmethyl-clarithromycin in chicken liver microsomes. The N-desmethyl-erythromycin A and N-desmethyl-clarithromycin were tentatively identified in chicken liver microsomes using the established quick analytic method, which will provide a theoretical foundation for future research on pharmacokinetics and drug elimination in poultry.
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Gu, Yi, Guangji Wang, and Jianguo Sun. "Simultaneous determination of erythromycin ethylsuccinate and its metabolite erythromycin in human plasma using liquid chromatography–electrospray ionization mass spectrometry for clinical study." Journal of Pharmaceutical and Biomedical Analysis 40, no. 3 (February 2006): 737–43. http://dx.doi.org/10.1016/j.jpba.2005.11.005.

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Billedeau, Stanley M., Thomas M. Heinze, and Paul H. Siitonen. "Liquid Chromatography Analysis of Erythromycin A in Salmon Tissue by Electrochemical Detection with Confirmation by Electrospray Ionization Mass Spectrometry." Journal of Agricultural and Food Chemistry 51, no. 6 (March 2003): 1534–38. http://dx.doi.org/10.1021/jf0209138.

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Adjei, Michael D., Thomas M. Heinze, Joanna Deck, James P. Freeman, Anna J. Williams, and John B. Sutherland. "Acetylation and nitrosation of ciprofloxacin by environmental strains of mycobacteria." Canadian Journal of Microbiology 53, no. 1 (January 1, 2007): 144–47. http://dx.doi.org/10.1139/w06-101.

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To determine the ability of environmental bacteria to metabolize the frequently prescribed fluoroquinolone drug ciprofloxacin, eight Mycobacterium spp. cultures were grown for 4 days in a medium containing sorbitol and yeast extract with 100 mg·L–1ciprofloxacin. After the cultures had been centrifuged and the supernatants extracted with ethyl acetate, two metabolites were purified by using high-performance liquid chromatography. They were identified with liquid chromatography/electrospray ionization mass spectrometry and proton nuclear magnetic resonance spectroscopy. Ciprofloxacin was transformed to both N-acetylciprofloxacin (2.5%–5.5% of the total peak area at 280 nm) and N-nitrosociprofloxacin (6.0%–8.0% of the peak area) by Mycobacterium gilvum PYR-GCK and Mycobacterium sp. PYR100 but it was transformed only to N-acetylciprofloxacin by Mycobacterium frederiksbergense FAn9, M. gilvum ATCC 43909, M. gilvum BB1, Mycobacterium smegmatis mc2155, Mycobacterium sp. 7E1B1W, and Mycobacterium sp. RJGII-135. The results suggest that biotransformation may serve as a ciprofloxacin resistance mechanism for these bacteria.Key words: acetylation, ciprofloxacin, fluoroquinolones, Mycobacterium, nitrosation.
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Demeiry, Menna El, Ahmed Ali, Yasmine Abouleila, Walaa Zarad, Heba El-Gendy, Randa Abdel Salam, Ghada Hadad, Yoshihiro Shimizu, Tsutomu Masujima, and Samy Emara. "Direct infusion nano-electrospray ionization mass spectrometry for therapeutic drug monitoring of ciprofloxacin and its metabolites in human saliva." Journal of Pharmaceutical and Biomedical Analysis 195 (February 2021): 113866. http://dx.doi.org/10.1016/j.jpba.2020.113866.

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Lai, Chien-Chen, Pei-Lun Tsai, Cheng Yu, and Guor-Rong Her. "Analysis of a commercial preparation of erythromycin estolates by tandem mass spectrometry and high performance liquid chromatography/electrospray ionization tandem mass spectrometry using an ion trap mass spectrometer." Rapid Communications in Mass Spectrometry 14, no. 6 (March 31, 2000): 468–75. http://dx.doi.org/10.1002/(sici)1097-0231(20000331)14:6<468::aid-rcm897>3.0.co;2-4.

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Khan, Aman Ullah, Waleed S. Shell, Falk Melzer, Ashraf E. Sayour, Eman Shawkat Ramadan, Mandy C. Elschner, Amira A. Moawad, Uwe Roesler, Heinrich Neubauer, and Hosny El-Adawy. "Identification, Genotyping and Antimicrobial Susceptibility Testing of Brucella spp. Isolated from Livestock in Egypt." Microorganisms 7, no. 12 (November 22, 2019): 603. http://dx.doi.org/10.3390/microorganisms7120603.

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Brucellosis is a highly contagious zoonosis worldwide with economic and public health impacts. The aim of the present study was to identify Brucella (B.) spp. isolated from animal populations located in different districts of Egypt and to determine their antimicrobial resistance. In total, 34-suspected Brucella isolates were recovered from lymph nodes, milk, and fetal abomasal contents of infected cattle, buffaloes, sheep, and goats from nine districts in Egypt. The isolates were identified by microbiological methods and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Differentiation and genotyping were confirmed using multiplex PCR for B. abortus, Brucella melitensis, Brucella ovis, and Brucella suis (AMOS) and Bruce-ladder PCR. Antimicrobial susceptibility testing against clinically used antimicrobial agents (chloramphenicol, ciprofloxacin, erythromycin, gentamicin, imipenem, rifampicin, streptomycin, and tetracycline) was performed using E-Test. The antimicrobial resistance-associated genes and mutations in Brucella isolates were confirmed using molecular tools. In total, 29 Brucella isolates (eight B. abortus biovar 1 and 21 B. melitensis biovar 3) were identified and typed. The resistance of B. melitensis to ciprofloxacin, erythromycin, imipenem, rifampicin, and streptomycin were 76.2%, 19.0%, 76.2%, 66.7%, and 4.8%, respectively. Whereas, 25.0%, 87.5%, 25.0%, and 37.5% of B. abortus were resistant to ciprofloxacin, erythromycin, imipenem, and rifampicin, respectively. Mutations in the rpoB gene associated with rifampicin resistance were identified in all phenotypically resistant isolates. Mutations in gyrA and gyrB genes associated with ciprofloxacin resistance were identified in four phenotypically resistant isolates of B. melitensis. This is the first study highlighting the antimicrobial resistance in Brucella isolated from different animal species in Egypt. Mutations detected in genes associated with antimicrobial resistance unravel the molecular mechanisms of resistance in Brucella isolates from Egypt. The mutations in the rpoB gene in phenotypically resistant B. abortus isolates in this study were reported for the first time in Egypt.
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Wilcox, Sheri K., Gregory S. Cavey, and James D. Pearson. "Single Ribosomal Protein Mutations in Antibiotic-Resistant Bacteria Analyzed by Mass Spectrometry." Antimicrobial Agents and Chemotherapy 45, no. 11 (November 1, 2001): 3046–55. http://dx.doi.org/10.1128/aac.45.11.3046-3055.2001.

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ABSTRACT Mutations in several ribosomal proteins are known to be related to antibiotic resistance. For several strains of Escherichia coli, the mutated protein is known but the amino acid actually altered has not been documented. Characterization of these determinants for antibiotic resistance in proteins will further the understanding of the precise mechanism of the antibiotic action as well as provide markers for resistance. Mass spectrometry can be used as a valuable tool to rapidly locate and characterize mutant proteins by using a small amount of material. We have used electrospray and matrix-assisted laser desorption ionization–time of flight (MALDI–TOF) mass spectrometry to map out all 56 ribosomal proteins in E. coli based on intact molecular masses. We used this fingerprinting approach to locate variants of ribosomal proteins displaying a change in mass. In particular we have studied proteins responsible for streptomycin, erythromycin, and spectinomycin resistance in three strains of E. coli, and then we characterized each mutation responsible for resistance by analyzing tryptic peptides of these proteins by using MALDI-TOF and nanoelectrospray tandem mass spectrometry. The results provided markers for antibiotic resistance and demonstrated that mass spectrometry can be used to rapidly investigate changes in individual proteins from a complex with picomole amounts of protein.
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WAMBUI, JOSEPH, TAURAI TASARA, PATRICK MURIGU KAMAU NJAGE, and ROGER STEPHAN. "Species Distribution and Antimicrobial Profiles of Enterococcus spp. Isolates from Kenyan Small and Medium Enterprise Slaughterhouses." Journal of Food Protection 81, no. 9 (August 3, 2018): 1445–49. http://dx.doi.org/10.4315/0362-028x.jfp-18-130.

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ABSTRACT The present study aimed at identifying and assessing antimicrobial resistance of Enterococcus spp. isolated from small and medium enterprise slaughterhouses in Kenya. In total, 67 isolates were recovered from 48 of 195 samples examined from beef carcasses, personnel, and cutting equipment in five small and medium enterprise slaughterhouses. The isolates were identified by using matrix-assisted laser desorption–ionization time of flight mass spectrometry and screened thereafter for their resistance against 12 antibiotics by using a disk diffusion assay. The isolates (n = 67) included Enterococcus faecalis (41.8%), Enterococcus mundtii (17.9%), Enterococcus thailandicus (13.4%), Enterococcus faecium (9.0%), Enterococcus hirae (7.5%), Enterococcus casseliflavus (6.0%), and Enterococcus devriesei (4.5%). None of the isolates were resistant to ciprofloxacin, penicillin, ampicillin, vancomycin, nitrofurantoin, teicoplanin, linezolid, and levofloxacin. Resistance to rifampin (46.3%), erythromycin (23.9%), tetracycline (20.9%), and chloramphenicol (7.5%) was distributed among six of the seven species. All E. thailandicus were resistant to rifampin, erythromycin, and tetracycline. E. faecalis was resistant to rifampin (60.7%), tetracycline (17.9%), erythromycin (14.3%), and chloramphenicol (10.7%). Resistance to two or three antibiotics was observed in 26.9% of the enterococci isolates. The isolation of enterococci that are resistant to clinically relevant antibiotics, such as erythromycin, is of a serious concern given the role enterococci play in the transfer of antibiotic resistance genes.
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Dissertations / Theses on the topic "Ciprofloxacin Erythromycin Electrospray ionization mass spectrometry"

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Achberger, Susan Lynn. "Analysis of alkali metal-catonized pharmaceuticals using electrospray ionization tandem mass spectrometry." Diss., 2008.

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Thesis (M.S.)--Michigan State University. Dept. of Biochemistry and Molecular Biology, 2008.
Title from PDF t.p. (viewed on July 29, 2009) Includes bibliographical references (p. 78-81). Also issued in print.
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