Journal articles on the topic 'Circuit edit'

For designing hardware with a high-level synthesis tool using a programming language such as C or Java, its large size of logic circuit makes it difficult to implement the design in a single FPGA. In such a case, partitioning the logic circuit and implementing in multiple FPGAs is a commonly used approach.
 We propose the Scalable Hardware Mechanism, which enables the operation of a partitioned circuit to prevent the degradation of clock frequency by minimizing its dependence on the usage and the type of FPGA. Our mechanism provides a reduced delay by the collective signal transmission with the partitioned AES code generation circuit and the character string edit distance calculation circuit as partitioned circuits. The collective signal transmission has attained 1.27 times improvement in the speed for the AES code generation circuit and 3.16 times improvement for the character string edit distance calculation circuit compared with the circuit by the conventional method.

Abstract. Physical analysis for IC functionality in submicron technologies requires access through chip backside. Based upon typical global backside preparation with 50–100 µm moderate silicon thickness remaining, a state of the art of the analysis techniques available for this purpose is presented and evaluated for functional analysis and layout pattern resolution potential. A circuit edit technique valid for nano technology ICs, is also presented that is based upon the formation of local trenches using the bottom of Shallow Trench Isolation (STI) as endpoint for Focused Ion Beam (FIB) milling. As a derivative from this process, a locally ultra thin silicon device can be processed, creating a back surface as work bench for breakthrough applications of nanoscale analysis techniques to a fully functional circuit through chip backside. Several applications demonstrate the power and potential of this new approach.

Background: The present study explores a novel self-pipelining strategy that can enhance speed-power efficiency as well as the reliability of a binary multiplier as compared to state-of-art register and wavepipelining. Method: Proper synchronization with efficient clocking between the subsequent self-pipelining stages has been assured to design a self-pipelined multiplier. Each self-pipelining stage consists of self-latching leaf cells that are designed, optimized and evaluated by TSMC 0.18μm CMOS technology with 1.8V supply rail and at 25°C temperature. The T-Spice transient response and simulated results for the designed circuits are presented. The proposed idea has been applied to design 4-b×4-b self-pipelined Wallace- tree multiplier. The multiplier was validated for all possible test patterns and the transient response was evaluated. The circuit performance in terms of propagation delay, average power and Power-Delay- Product (PDP) is recorded. Next, the decomposition logic is applied to design a higher-order multiplier (i.e., 8-bit×8-bit and 16-bit×16-bit) based on the proposed strategy using 4-bit×4-bit self-pipelined multiplier. The designed multiplier was also validated through extensive TSpice simulation for all the required test patterns using W-Edit and the evaluated performance is presented. All the designs, optimizations and evaluations performed are based on BSIM3 device parameter of TSMC 0.18μm CMOS technology with 1.8V supply rail at 25°C temperature using S-Edit of Tanner EDA. Results: The reliability was investigated of the proposed 4-b×4-b multiplier in the temperature range - 40°C to 100°C for maximum PDP variation. Conclusion: A benchmarking analysis in terms of speed-power performance with recent competitive design reveals preeminence of the proposed technique.

To overcome the shortcomings of external PLC (Programmable Logic Controller) and soft PLC being widely employed in switch control of CNC machine tool, an embedded PLC system was developed by integrating AVR single chip ATMEGA169, PC bus technique and dual port RAM. Both hardware circuit and software of the PLC system were designed and implemented. The software of Industrial PC was developed by using ladder-diagram IDE (Integrated Design Environment) based on the platform of Borland C++ Builder. In this environment, it can fulfill edit, compilation and communication function of ladder-diagram file. It also can monitor and diagnose PLC running status. This developed embedded PLC system has the characteristics of compact structure, high communication and reliability. It can effectively satisfy real-time control requirements of CNC machine tool.

Purpose – Flexible light-emitting textile display is designed with floats for electronic elements covering and electronic contacts insulation what at the same time provides an opportunity to develop aesthetic design of the display in the single piece construction of material. The paper aims to discuss these issues. Design/methodology/approach – Display consists of interwoven electrically conductive yarns, non-conductive yarns and SMD LEDs connected to conductive yarns. Industrial jacquard weaving machine have been used, weave patterns were designed in PC-Edit software. Findings – Weave can be used as a tool to build and evolve electrotextile. Exploring weaving techniques and perceiving electronic circuit as a weave pattern, new approaches can be developed in electrotextile design field. Research limitations/implications – Connections of electronic elements and conductive textile materials still is actual problem what should be explored in further research. Practical implications – Flexible light emitting textile display can be used as output interface integrated into communication clothing by representing different animated images directly on clothing. Display also can be used for accessories, room and auto interior etc. applications. Originality/value – Paper describes method of light source integration directly into textile structure, combining functional and visual design of textile display.

In this contribution, we compare the performance of Focused Electron Beam-induced Deposition (FEBID) and Focused Ion Beam-induced Deposition (FIBID) at room temperature and under cryogenic conditions (the prefix “Cryo” is used here for cryogenic). Under cryogenic conditions, the precursor material condensates on the substrate, forming a layer that is several nm thick. Its subsequent exposure to a focused electron or ion beam and posterior heating to 50 °C reveals the deposit. Due to the extremely low charge dose required, Cryo-FEBID and Cryo-FIBID are found to excel in terms of growth rate, which is typically a few hundred/thousand times higher than room-temperature deposition. Cryo-FIBID using the W(CO)6 precursor has demonstrated the growth of metallic deposits, with resistivity not far from the corresponding deposits grown at room temperature. This paves the way for its application in circuit edit and the fast and direct growth of micro/nano-electrical contacts with decreased ion damage. The last part of the contribution is dedicated to the comparison of these techniques with other charge-based lithography techniques in terms of the charge dose required and process complexity. The comparison indicates that Cryo-FIBID is very competitive and shows great potential for future lithography developments.

FPGA device is a dominant implementation medium for digital circuits. Unfortunately, they do not support asynchronous circuits because of the lack of asynchronous circuit elements such as Muller gates, etc. In this paper, new efficient approaches are proposed to prototype asynchronous circuits on Look-Up Table-based (LUT) FPGA rapidly. The developed techniques are based on building of elements which play an important role in asynchronous circuits. The hazard-free elements are predefined in libraries in HDL and EDIF format. Timing and/or area constraints for place&route tool are automatically generated to map the asynchronous elements on suitable FPGA’s logic blocks. Several FPGA devices such as Altera, Xilinx and Actel could be used as target for the implementation.

As the demand on Computer-Aided Testing Systems (CATS)—Automatic Test Pattern Generation (ATPG) and logic and fault simulations as well as testability analysis—increases and the choice becomes more varied, a need to compare the merits of the different systems emerges. Benchmark circuits are used to carry out the comparisons.In this paper, criteria for selecting the benchmark circuits are discussed. These criteria are partly based on the results of experiments carried out to characterize CATS. The focus is particularly on Automatic Test Pattern Generators. The preliminary results show that there is no general agreement on how: 1) fault collapsing is performed, and 2) fault coverage is calculated. In addition, the performance of the ATPGs depends on the circuit representation, topology and size as well as the algorithm. In order to compare the performance of the ATPGs as the circuit under test increases in complexity, it is important to use regular structures that consist of replication of medium size circuits. Practical considerations involved in benchmarking are also examined. Emphasis is on the transfer of circuits between different CATS systems and the use of EDIF as a neutral exchange language.

Las revistas científicas se han consolidado como parte neurálgica de los regímenes de evaluación de la ciencia contemporánea. En este contexto, nos interesa aproximarnos a un espacio de circulación nacional concreto para advertir hasta qué punto la tan remanida globalización de la ciencia ha impactado en circuitos locales y regionales. En este artículo nos proponemos indagar la morfología del espacio argentino de revistas científicas, su constitución histórica y actual mapeo espacio-institucional. Para ello analizamos un universo de 1208 revistas científicas indexadas y no indexadas activas al año 2016/2017. Como resultado observamos la coexistencia de diferentes formas simultáneas y diversas de circulación de revistas. Particularmente analizamos dos tipos particulares de circuitos orientados localmente: 1) un circuito con una larga tradición de edición, orientado disciplinarmente por las ciencias exactas y naturales, editados mayoritariamente en papel y sin indexación; 2) y otro, con una tradición de edición desde mediados de 1990, que edita digitalmente y se desarrolla en el seno de grandes universidades nacionales. Proponemos una conceptualización guiada por la noción de capital social para comprender la subsistencia de estos circuitos locales en el marco de las culturas evaluativas nacionales y de la competencia científica internacional.

The discovery and adaption of bacterial clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas) systems has revolutionized the way researchers edit genomes. Engineering of catalytically inactivated Cas variants (nuclease-deficient or nuclease-deactivated [dCas]) combined with transcriptional repressors, activators, or epigenetic modifiers enable sequence-specific regulation of gene expression and chromatin state. These CRISPR–Cas-based technologies have contributed to the rapid development of disease models and functional genomics screening approaches, which can facilitate genetic target identification and drug discovery. In this short review, we will cover recent advances of CRISPR–dCas9 systems and their use for transcriptional repression and activation, epigenome editing, and engineered synthetic circuits for complex control of the mammalian genome.

Learning to read involves the acquisition of letter–sound relationships (i.e., decoding skills) and the ability to visually recognize words (i.e., orthographic knowledge). Although decoding skills are clearly human-unique, given they are seated in language, recent research and theory suggest that orthographic processing may derive from the exaptation or recycling of visual circuits that evolved to recognize everyday objects and shapes in our natural environment. An open question is whether orthographic processing is limited to visual circuits that are similar to our own or a product of plasticity common to many vertebrate visual systems. Here we show that pigeons, organisms that separated from humans more than 300 million y ago, process words orthographically. Specifically, we demonstrate that pigeons trained to discriminate words from nonwords picked up on the orthographic properties that define words and used this knowledge to identify words they had never seen before. In addition, the pigeons were sensitive to the bigram frequencies of words (i.e., the common co-occurrence of certain letter pairs), the edit distance between nonwords and words, and the internal structure of words. Our findings demonstrate that visual systems organizationally distinct from the primate visual system can also be exapted or recycled to process the visual word form.

AbstractThea.d.362/3 crisis in Antioch is usually interpreted as an economic or ideological crisis, and Julian'sMisopogonas a ‘festive satire’ or ‘edict of chastisement’. This article situates the root of the problem in a crisis of communication: Julian's failure to communicate publicly as expected in a situation that was tense because of the food shortage led to a short-circuit between emperor and subjects. Whilst theMisopogonis Julian's extraordinarypost-factumattempt to explain away this failure of ritualized communication on his part, Libanius’ speeches on the topic seek to give a positive twist to the extraordinary nature of Julian's reply, which posed serious problems for emperor, city, and sophist alike.

A partir de um estudo de caso centrado em uma organização que edita uma revista vendida por pessoas "sem teto" na cidade de Buenos Aires, me interrogo sobre os usos sociais do dinheiro em circuitos filantrópicos. Ao seguir a polêmica entre dinheiro e dom, que está no centro das discussões da antropologia e da sociologia, enfoco duas questões: a) o papel do dinheiro para demarcar os limites entre as "transações mercantis" e as "circulações de dons"; b) a relação entre dinheiro e moral. O trabalho de campo consistiu em uma etnografia das transações entre os vendedores e os compradores desta publicação.

The paper reveals the dependences of the output dynamic characteristics of semiconductor electric discharge installations (EDIs) with reservoir capacitors on the features of the change in the value of their capacitance. In particular, it is substantiated that for any fixed discharge duration less than the duration of reaching the maximum discharge current, an increase in the capacitance of such storage devices causes an increase in the value of the final discharge current both at aperiodic and oscillatory discharges in the linear resistance of the technological load. The change in the value of the discharge current in the load in the case of forced interruption of this current at a certain moment of time is investigated. Based on the obtained regularities, the authors of the work proposed to use the capacitance of the EDI's capacitor, which is larger than capacitance required to implement the maximum value of the discharge current in the load. Using a capacitor with a larger capacity and a fully controlled semiconductor switch in the discharge circuit of the EDI, it is possible to obtain the required maximum current value at a shorter duration of the discharge process. Thus, it is possible to regulate the main dynamic parameters of pulse currents in the load – the rate of their rise and/or their duration by changing the value of the capacitance of the discharge capacitor EDI. This approach is expedient for increasing the productivity of EDIs, focused on the production of dispersed spark powders of metals and alloys. References 15, figures 3.

Background: A novel system for the usage of Maximum Power Point Tracking of an expansive Solar Photo Voltaic (SPV) farm subjected to conceivable incomplete shading is displayed in this paper. The SPV farm being spread over an expansive territory a remote sensor organize is utilized for checking the sun based protection in the region of each board. The motivation behind the remote sensor organize is to screen the sunlight based protection at various areas near each of the PV board from the tremendous region of the photograph voltaic homestead comprising of countless voltaic boards. The observed protection information is utilized by a prepared. Artificial Neural Network to locate the ideal DC terminal voltage to be kept up over the general DC terminals of the photograph voltaic ranch. All the PV boards are associated in arrangement association with the fundamental bye pass diodes. The DC control accessible at the yield terminals of the SPV cultivate is first DC to DC changed over with a Positive Output Luo Converter (POLC) and bolstered to a heap. A MATLAB Simulink based reproduction was created to approve the proposed system. Methods: Maximum Power Point Tracking based on Artificial Neural Network through wireless sensor networks. Results: As the result of the proposed idea and its implementation in MATLAB we have two sets of results. In either case the input is a vector of 40 elements and the output of the first segment of the work is the estimation of the threshold PV terminal voltage that will guarantees maximum power point operation. In the first case we have the MATLAB SIMULINK implementation of the basic configuration of the forty PV panels arranged in series connection and we have provided a facility to edit the solar insulation levels pertaining to the individual PV panels. In this first configuration we have set a continuously variable PV current for all the panels and the PV current for all the panel are the same. Using this setup, for any combination of solar insulation pattern of the forty panels the overall PV curve and the overall VI curve can be drawn in MATLAB. As the simulation runs the PV current is changed from 0 to the maximum or the short circuit current level in a slowly rising manner implemented using a ramp signal. </P><P> During this period the total power output and the terminal voltage of the PV farm are sent to the work space and the data is thus collected in the workspace of MATLAB. Using basic MATLAB commands the maximum power output and the PV terminal voltage corresponding to the maximum power output are obtained. The PV current at maximum power output condition, the corresponding PV farm terminal voltage, the maximum power output recorded at this condition all correspond to the present insulation vector condition. This way, by changing the elements of the insulation for all the forty panels in a random manner we obtain for each case the Ipmax[i], Pmax[i], Vpmax[i] and this corresponds to insulation[n,i]. Where n is the number of panels, in this case 40 and i the ith experiment. In each experiment the solar insulation level of all the forty panels can be changed and the parameters Vpmax[i], Ipmax[i] and Pmax[i] can be obtained. The value of the harvested power as found from the characteristics for any given set of insulation is denoted as the estimated power. The value of power as obtained from the proposed ANN SMC POLC combination is denoted as the Actual Power. Conclusion: A wireless network based insulation monitoring has been done. An ANN based MPPT algorithm has been developed that gives the reference MPP voltage. The sliding mode control scheme uses the reference voltage and produces the switching pulses for the POLC. The ANN had been trained with a number of combinations of different insulation values falling on each of the forty panels and the ANN gives the correct reference voltage for any combination of insulation levels that were not used while training. The sliding mode controller uses this reference voltage and gives the switching pulses to the POLC that harvests the maximum power output to the RL load. The proposed system has been implemented in the MATLAB SIMULINK environment and has thus been validated. The obtained results have been compared against the maximum power output values that could be derived from the characteristic curves obtained for the given combination of insulation levels. The proposed system gives results very close to the values obtained from the characteristics. As a future work the proposed idea can be validated using hardware based experimental setup.

El fotógrafo, periodista, escritor y cineasta Alejandro Del Conte es hoy célebre por su rol fundamental en el desarrollo, difusión y profesionalización de la fotografía artística en la Argentina. Poco se sabe, sin embargo, de los variados y precursores proyectos que este personaje multifacético desarrolló en el ámbito de la producción, distribución, exhibición y, sobre todo, de la crítica cinematográfica regional. En efecto, además de exhibir, alquilar y dirigir películas en el norte del país y en Buenos Aires, en 1917 Del Conte fundó y editó en Tucumán una publicación gremial dedicada al cine, pionera y hoy casi desconocida: <em>Film Gráfico. Revista Semanal Cinematográfica del Norte de la República</em>. Basándonos en su correspondencia y archivo personal y en los pocos ejemplares sobrevivientes de esta publicación, en este trabajo proponemos no solo reconstruir este único y ambicioso proyecto editorial, sino también el papel que esta publicación tuvo en la creación y desarrollo de un temprano proyecto para instaurar un circuito comercial regional cuyo centro no estaba en la Capital sino en Rosario, ciudad que muy pronto se convertiría en el segundo polo cinematográfico de importancia en el país durante el período silente

With the shrinking feature size and increasing aspect ratios of interconnects in DSM chips, the coupling noise between adjacent interconnects has become a major signal integrity (SI) issue, giving rise to crosstalk failures. In older technologies, SI issues have been ignored because of high noise immunity of the CMOS circuits and the process technology. However, as CMOS technologies lower down the supply voltage as well as the threshold voltage of a transistor, digital designs are more and more susceptible to noise because of the reduction of noise margin. The genetic algorithms (GAs) have been applied earlier in different engineering disciplines as potentially good optimization tools and for various applications in VLSI design, layout, EDIF digital system testing and also for test automation, particularly for stuck-at-faults and crosstalk-induced delay faults. In this paper, an elitist GA has been developed that can be used as an ATPG tool for generating the test patterns for crosstalk-induced faults between on-chip aggressor and victim and as well as for stuck-at-faults. It has been observed that the elitist GA, when the fitness function is properly defined, has immense potential in extracting the suitable test vectors quickly from randomly generated initial patterns.

Urgency of the research. Computer models mean new quality in the knowledge process. Using a computer model, the properties of the subject under investigation can be tested under different operating conditions. By experimenting with a com-puter model, we learn about the modelled object. We can test different machine variants without having to produce and edit prototypes. Target setting. The development of computer technology has expanded the possibility of solving mathematical models and allowed to gradually automate the calculation of mathematical model equations. It is necessary to insert appropriate inputs of the mathematical model and monitor and evaluate the output results through the computer output device The target was to describe the mathematical apparatus required for mathematical modeling and subsequently to compile a model for computer modeling. Actual scientific researches and issues analysis. When formulating a mathematical model for a computer, the laws and the theory we use are always valid under more or less idealized conditions, and operate with fictitious concepts such as, material point, ideal gas, intangible spring, and the like. However, with these simplifications, we describe a realistic phenomenon where the initial assumptions are only met to a certain extent. In order for the results not to be different from the modeled reality, it is to be assumed that a good computer model arises gradually, by verifying and modifying it, which is one of the advantages of MSC Adams. Uninvestigated parts of general matters defining. The question of building a real manipulator model. Based on the above simulation, it is possible to build a real model. The research objective. Using MSC Adams to simulate multiple body systems and verify its suitability for simulating ma-nipulator and robot models. In various versions of the assembled model we can monitor its behavior under different operating conditions. The statement of basic materials. In computer simulation, MSC Adams-View is used to simulate mechanical systems. It has an interactive environment for automated dynamic analysis of parameterized mechanical systems with an arbitrary struc-ture of rigid and flexible bodies with geometric or force joints, in which act gravity, inertia, experimentally designed contact, friction, aerodynamic, hydrodynamic or electromechanical forces and have integrated control, hydraulic, pneumatic or elec-tromechanical circuits. Conclusions. Working with a mathematical model on a computer opens space for specific synthesis of empirical and ana-lytical method of scientific knowledge. Working with the computer model carries the characteristic features of classical experi-mentation. It represents a qualitatively new way of solving tasks that can not be experimented with on a real object. The result is the equivalence of the computer model and the object being investigated with the features and expressions chosen as essen-tial, with accuracy sufficient to the exact purpose.

Induced pluripotent stem cell (iPSC)-derived natural killer (iNK) cells offer a promising platform for off-the-shelf immunotherapy against cancer. A unique benefit of iPSC-derived immune effector cells is the possibility to perform multiple precision editing steps at the single cell level to achieve a homogenous effector cell population tailored to target a desired cancer type and equipped with selected functional properties. These functional edits are superimposed on the innate reactivity of NK cells to stress ligands and MHC downregulation (missing self). The ability of NK cells to sense missing self is based on a functional calibration to self MHC during a process termed NK cell education, the latter being critically dependent on signaling through inhibitory receptors, including CD94/NKG2A and killer cell immunoglobulin-like receptors (KIR). Whereas the process of NK cell differentiation into mature effector cells from iPSCs has been well characterized, the role of natural variation in inhibitory receptor expression and NK cell education remains poorly defined in iNK cells. We used mass cytometry to map the receptor repertoire in series of iNK cell lines and genetic edits thereof during differentiation and in vitro expansion (Figure 1A and B). Similar to peripheral blood NK cells, the receptor repertoire was diversified but genetically hardwired showing consistent patterns within each iNK cell line but with slight variation between genetically distinct lines. NKG2A was the dominantly expressed inhibitory receptor ranging from 13% to 87% with the highest expression in multi-edited iNK cell lines engineered to express a chimeric antigen receptor against CD19, a high affinity, non-cleavable FcγRIIIa receptor (CD16) and a recombinant IL15 signaling complex (CAR19-iNK cells). KIR expression was generally low in all tested iNK cell lines but increased gradually during culture and was further increased by genetic silencing of NKG2A receptors. Interestingly, silencing of NKG2A lead to increased levels of the activating receptor NKG2C. We monitored degranulation by iNK cell variants against K562 engineered to express varying levels of HLA-E as well as CD19+ Nalm-6 cells. Genetic silencing of ß2microglobulin (ß2m), associated with reduced levels of HLA-class I and HLA-E, led to dampened global functional responses in iNK cells, suggesting a positive impact of education during iNK cell differentiation and expansion (Figure 1C). Subset stratification revealed that NKG2A+ iNK cells showed superior functionality compared to NKG2A- iNK cells across all iNK cell lines tested, albeit less striking in CAR19-iNK cells that showed the highest overall natural cytotoxicity (Figure 1D). Knockdown of NKG2A led to a general reduction in functional capacity of NK92 cells (Figure 1E-F) and CAR19-iNK cells (Figure 1H), supporting a critical role for NKG2A-driven education in iNK cells. Given the superior functionality of NKG2A+ iNK cells, we next addressed whether this advantage was countered by expression of the check point ligand HLA-E during target cell interactions. Although we noted a slight inhibitory impact on natural cytotoxicity in NK cells isolated and expanded from peripheral blood (PB-NK) against K562 cells expressing physiological levels of HLA-E, this effect was completely overridden in iNK cells and did not interfere with NKG2A+ CAR-iNK cell recognition of HLA-E expressing CD19+ target cells (Figure 1G-H). Indeed, NKG2A+ CAR19-iNK showed superior degranulation against HLA-E expressing CD19+ Nalm-6 targets compared to CRISPR-edited NKG2A-/- CAR19-iNK cells (Figure 1I). Our results shed light on the regulatory gene circuits and cellular programs that determine functional potential in iPSC-derived NK cells products. Specifically, our results point to a crucial role for NKG2A-driven acquisition of a mature effector cell phenotype in combination with functional education through cognate ligands. Importantly, iNK cell education is operational during iNK cell differentiation and expansion without interfering with recognition of tumor targets expressing HLA-E. Figure 1 Disclosures Cichocki: Fate Therapeutics, Inc: Consultancy, Patents & Royalties, Research Funding. Mahmood:Fate Therapeutics, Inc: Current Employment. Gaidarova:Fate Therapeutics, Inc: Current Employment. Bjordahl:Fate Therapeutics: Current Employment. Chu:Fate Therapeutics, Inc: Current Employment. Groff:Fate Therapeutics, Inc: Current Employment. Denholtz:Fate Therapeutics, Inc: Current Employment. Miller:Fate Therapeutics, Inc: Consultancy, Patents & Royalties, Research Funding; Vycellix: Consultancy; Onkimmune: Honoraria, Membership on an entity's Board of Directors or advisory committees; Nektar: Honoraria, Membership on an entity's Board of Directors or advisory committees; GT Biopharma: Consultancy, Patents & Royalties, Research Funding. Lee:Fate Therapeutics, Inc.: Current Employment. Kaufman:Fate Therapeutics: Consultancy. Goodridge:Fate Therapeutics, Inc: Current Employment. Valamehr:Fate Therapeutics, Inc: Current Employment, Current equity holder in publicly-traded company. Malmberg:Fate Therapeutics: Consultancy, Patents & Royalties; Vycellix: Membership on an entity's Board of Directors or advisory committees.

Automation is one of the growing fields ,and is being used at levels from small scale industries to very a large scale industries due to the advantage of increase in productivity and quality, along with it recently new revolution in the automation 4.0enabling the data monitoring possible which helps in better control and monitoring of machineries and equipments. The objective of the work is to design electrical circuit and perform suitable control actions such as loading and unloading of heavier components to machines, indexing, providing suitable safety for the devices and improvising productivity and quality rate in the production line. These activities are being done by a control system adopted to gantry system, in such control systems there will be provision for manual control, auto mode, jog mode & edit mode to enable the work to be carried out smoothly and effectively. Such complete system is designed to reach the customer required production cycle time with 6 axis (y,z1,z2,x1,x2,c), these axis are controlled by CNC controller and all other Stations like IPC, OPC, tilting stations are controlled by the PMC controller, which is the part of CNC.

On July 10, 1926, the First-President of the Lăpușna Court, judge Eugen Ionescu-Dârzeu and the regional manager of the Romanian Archives, L. T. Boga, signed the acceptance protocol for the old archive of the Lăpușna Court, which comprised around 2,345 documents. During the inventorying process, L. T. Boga and the archivists in Kishinev typed several copies of an opis, of which one (copy No. 3)—which I feature here—ended up in the collections of the Romanian Academy Library, probably from the archive of judge E. Ionescu-Dârzeu. Currently, this collection was integrated to Fund 220 within the Kishinev Archives. Following a global appraisal of the documents published from this archive, I may state that around a quarter of them (approximately 500) were edited, mainly by L. T. Boga and by the editors of the collection Moldova în epoca feudalismului. It is the responsibility of present or future researchers to edit integrally or as abstracts, on a theme-based or chronology-based approach, other documents within this collection. Until the fulfilment of this desideratum, the critical editing of this opis introduces in the scientific circuit around 2,000 documents, as abstracts. This important archival work tool comprises unpublished information about the history of Moldova, mostly of the regions left from the Pruth River, before and after 1812, concerning both the political and social history and the local history, the toponymy or the genealogy.

In this article, displays the capacity of decrease the Power, Area in CMOS VLSI blocks. Power in CMOS circuits is mostly consumed for the duration of the transitions of the gates. Thusly, control estimation of CMOS circuits is changed over into progress action estimation. A few methods are utilized to mimic progress exercises of CMOS circuits. The proposed Vedic multiplier is planned by utilizing various strategies of full adder cells. The structure of full adders for low power is gotten and low power blocks are actualized on the arranged multiplier with the outcomes be broke down used for improved execution. The structures are finished by utilizing TANNER S-EDIT tool and recreated utilizing T-SPICE.

AbstractTemporal modulation of the expression of multiple genes underlies complex complex biological phenomena. However, there are few scalable and generalizable gene circuit architectures for the programming of sequential genetic perturbations. Here, we describe a modular recombinase-based gene circuit architecture, comprising tandem gene perturbation cassettes (GPCs), that enables the sequential expression of multiple genes in a defined temporal order by alternating treatment with just two orthogonal ligands. We use tandem GPCs to sequentially express single-guide RNAs to encode transcriptional cascades that trigger the sequential accumulation of mutations. We build an all-in-one gene circuit that sequentially edits genomic loci, synchronizes cells at a specific stage within a gene expression cascade, and deletes itself for safety. Tandem GPCs offer a multi-tiered cellular programming tool for modeling multi-stage genetic changes, such as tumorigenesis and cellular differentiation.

Los circuitos integrados VLSI (gran escala de integración), son aquellos que se caracterizan por sus dimensiones extremadamente pequeñas, constituidos principalmente por transistores de efecto de campo de metal óxido semiconductor (MOSFET), cuyas longitudes de canal son inferiores al micrómetro, permitiendo integrar un mayor número de transistores en un solo diseño. En el presente trabajo, se diseña una compuerta NAND basada en la tecnología CMOS en la herramienta L-Edit de Tanner Eda, realizando los cálculos matemáticos necesarios para la determinación de las relaciones W, L de los transistores MOSFET, siguiendo las reglas de diseño establecidas al momento del diseño del respectivo layout. Para la creación de bibliotecas de diseño y su posterior utilización en circuitos integrados más grandes y complejos.

There are several items that you should have to protect your family and home, such as: a fire extinguisher, smoke detectors, Ground Fault Circuit Interrupters (GFCIs), carbon monoxide detectors, and locks on your doors and windows. This document is FCS5233-05, one of a series of the Department of Family, Youth and Community Sciences, UF/IFAS Extension. Original publication date: May 2002. Revised: December 2005.

Abstract Autism spectrum disorder (ASD) is characterized by a triad of behavioural impairments including social behaviour. Neuroligin, a trans-synaptic adhesion molecule, has emerged as a penetrant genetic determinant of behavioural traits that signature the neuroatypical behaviours of autism. However, the function of neuroligin in social circuitry and the impact of genetic variation to this gene is not fully understood. Indeed, in animal studies designed to model autism, there remains controversy regarding the role of neuroligin dysfunction in the expression of disrupted social behaviours. The model organism, Caenorhabditis elegans, offers an informative experimental platform to investigate the impact of genetic variants on social behaviour. In a number of paradigms, it has been shown that inter-organismal communication by chemical cues regulates C. elegans social behaviour. We utilize this social behaviour to investigate the effect of autism-associated genetic variants within the social domain of the research domain criteria. We have identified neuroligin as an important regulator of social behaviour and segregate the importance of this gene to the recognition and/or processing of social cues. We also use CRISPR/Cas9 to edit an R-C mutation that mimics a highly penetrant human mutation associated with autism. C. elegans carrying this mutation phenocopy the behavioural dysfunction of a C. elegans neuroligin null mutant, thus confirming its significance in the regulation of animal social biology. This highlights that quantitative behaviour and precision genetic intervention can be used to manipulate discrete social circuits of the worm to provide further insight into complex social behaviour.

Writing in the American Annals of the Deaf in 1931, Emil S. Ladner Jr, a Deaf high school student, predicted the invention of words on screen to facilitate access to “talkies”. He anticipated:Perhaps, in time, an invention will be perfected that will enable the deaf to hear the “talkies”, or an invention which will throw the words spoken directly under the screen as well as being spoken at the same time. (Ladner, cited in Downey Closed Captioning)This invention would eventually come to pass and be known as captions. Captions as we know them today have become widely available because of a complex interaction between technological change, volunteer effort, legislative activism, as well as increasing consumer demand. This began in the late 1950s when the technology to develop captions began to emerge. Almost immediately, volunteers began captioning and distributing both film and television in the US via schools for the deaf (Downey, Constructing Closed-Captioning in the Public Interest). Then, between the 1970s and 1990s Deaf activists and their allies began to campaign aggressively for the mandated provision of captions on television, leading eventually to the passing of the Television Decoder Circuitry Act in the US in 1990 (Ellis). This act decreed that any television with a screen greater than 13 inches must be designed/manufactured to be capable of displaying captions. The Act was replicated internationally, with countries such as Australia adopting the same requirements with their Australian standards regarding television sets imported into the country. As other papers in this issue demonstrate, this market ultimately led to the introduction of broadcasting requirements.Captions are also vital to the accessibility of videos in today’s online and streaming environment—captioning is listed as the highest priority in the definitive World Wide Web Consortium (W3C) Web Content Accessibility Guideline’s (WCAG) 2.0 standard (W3C, “Web Content Accessibility Guidelines 2.0”). This recognition of the requirement for captions online is further reflected in legislation, from both the US 21st Century Communications and Video Accessibility Act (CVAA) (2010) and from the Australian Human Rights Commission (2014).Television today is therefore much more freely available to a range of different groups. In addition to broadcast channels, captions are also increasingly available through streaming platforms such as Netflix and other subscription video on demand providers, as well as through user-generated video sites like YouTube. However, a clear discrepancy exists between guidelines, legislation and the industry’s approach. Guidelines such as the W3C are often resisted by industry until compliance is legislated.Historically, captions have been both unavailable (Ellcessor; Ellis) and inadequate (Ellis and Kent), and in many instances, they still are. For example, while the provision of captions in online video is viewed as a priority across international and domestic policies and frameworks, there is a stark contrast between the policy requirements and the practical implementation of these captions. This has led to the active development of a solution as part of an ongoing tradition of user-led development; user-generated captions. However, within disability studies, research around the agency of this activity—and the media savvy users facilitating it—has gone significantly underexplored.Agency of ActivityInformation sharing has featured heavily throughout visions of the Web—from Vannevar Bush’s 1945 notion of the memex (Bush), to the hacker ethic, to Zuckerberg’s motivations for creating Facebook in his dorm room in 2004 (Vogelstein)—resulting in a wide agency of activity on the Web. Running through this development of first the Internet and then the Web as a place for a variety of agents to share information has been the hackers’ ethic that sharing information is a powerful, positive good (Raymond 234), that information should be free (Levey), and that to achieve these goals will often involve working around intended information access protocols, sometimes illegally and normally anonymously. From the hacker culture comes the digerati, the elite of the digital world, web users who stand out by their contributions, success, or status in the development of digital technology. In the context of access to information for people with disabilities, we describe those who find these workarounds—providing access to information through mainstream online platforms that are not immediately apparent—as the disability digerati.An acknowledged mainstream member of the digerati, Tim Berners-Lee, inventor of the World Wide Web, articulated a vision for the Web and its role in information sharing as inclusive of everyone:Worldwide, there are more than 750 million people with disabilities. As we move towards a highly connected world, it is critical that the Web be useable by anyone, regardless of individual capabilities and disabilities … The W3C [World Wide Web Consortium] is committed to removing accessibility barriers for all people with disabilities—including the deaf, blind, physically challenged, and cognitively or visually impaired. We plan to work aggressively with government, industry, and community leaders to establish and attain Web accessibility goals. (Berners-Lee)Berners-Lee’s utopian vision of a connected world where people freely shared information online has subsequently been embraced by many key individuals and groups. His emphasis on people with disabilities, however, is somewhat unique. While maintaining a focus on accessibility, in 2006 he shifted focus to who could actually contribute to this idea of accessibility when he suggested the idea of “community captioning” to video bloggers struggling with the notion of including captions on their videos:The video blogger posts his blog—and the web community provides the captions that help others. (Berners-Lee, cited in Outlaw)Here, Berners-Lee was addressing community captioning in the context of video blogging and user-generated content. However, the concept is equally significant for professionally created videos, and media savvy users can now also offer instructions to audiences about how to access captions and subtitles. This shift—from user-generated to user access—must be situated historically in the context of an evolving Web 2.0 and changing accessibility legislation and policy.In the initial accessibility requirements of the Web, there was little mention of captioning at all, primarily due to video being difficult to stream over a dial-up connection. This was reflected in the initial WCAG 1.0 standard (W3C, “Web Content Accessibility Guidelines 1.0”) in which there was no requirement for videos to be captioned. WCAG 2.0 went some way in addressing this, making captioning online video an essential Level A priority (W3C, “Web Content Accessibility Guidelines 2.0”). However, there were few tools that could actually be used to create captions, and little interest from emerging online video providers in making this a priority.As a result, the possibility of user-generated captions for video content began to be explored by both developers and users. One initial captioning tool that gained popularity was MAGpie, produced by the WGBH National Center for Accessible Media (NCAM) (WGBH). While cumbersome by today’s standards, the arrival of MAGpie 2.0 in 2002 provided an affordable and professional captioning tool that allowed people to create captions for their own videos. However, at that point there was little opportunity to caption videos online, so the focus was more on captioning personal video collections offline. This changed with the launch of YouTube in 2005 and its later purchase by Google (CNET), leading to an explosion of user-generated video content online. However, while the introduction of YouTube closed captioned video support in 2006 ensured that captioned video content could be created (YouTube), the ability for users to create captions, save the output into one of the appropriate captioning file formats, upload the captions, and synchronise the captions to the video remained a difficult task.Improvements to the production and availability of user-generated captions arrived firstly through the launch of YouTube’s automated captions feature in 2009 (Google). This service meant that videos could be uploaded to YouTube and, if the user requested it, Google would caption the video within approximately 24 hours using its speech recognition software. While the introduction of this service was highly beneficial in terms of making captioning videos easier and ensuring that the timing of captions was accurate, the quality of captions ranged significantly. In essence, if the captions were not reviewed and errors not addressed, the automated captions were sometimes inaccurate to the point of hilarity (New Media Rock Stars). These inaccurate YouTube captions are colloquially described as craptions. A #nomorecraptions campaign was launched to address inaccurate YouTube captioning and call on YouTube to make improvements.The ability to create professional user-generated captions across a variety of platforms, including YouTube, arrived in 2010 with the launch of Amara Universal Subtitles (Amara). The Amara subtitle portal provides users with the opportunity to caption online videos, even if they are hosted by another service such as YouTube. The captioned file can be saved after its creation and then uploaded to the relevant video source if the user has access to the location of the video content. The arrival of Amara continues to provide ongoing benefits—it contains a professional captioning editing suite specifically catering for online video, the tool is free, and it can caption videos located on other websites. Furthermore, Amara offers the additional benefit of being able to address the issues of YouTube automated captions—users can benefit from the machine-generated captions of YouTube in relation to its timing, then download the captions for editing in Amara to fix the issues, then return the captions to the original video, saving a significant amount of time when captioning large amounts of video content. In recent years Google have also endeavoured to simplify the captioning process for YouTube users by including its own captioning editors, but these tools are generally considered inferior to Amara (Media Access Australia).Similarly, several crowdsourced caption services such as Viki (https://www.viki.com/community) have emerged to facilitate the provision of captions. However, most of these crowdsourcing captioning services can’t tap into commercial products instead offering a service for people that have a video they’ve created, or one that already exists on YouTube. While Viki was highlighted as a useful platform in protests regarding Netflix’s lack of captions in 2009, commercial entertainment providers still have a responsibility to make improvements to their captioning. As we discuss in the next section, people have resorted extreme measures to hack Netflix to access the captions they need. While the ability for people to publish captions on user-generated content has improved significantly, there is still a notable lack of captions for professionally developed videos, movies, and television shows available online.User-Generated Netflix CaptionsIn recent years there has been a worldwide explosion of subscription video on demand service providers. Netflix epitomises the trend. As such, for people with disabilities, there has been significant focus on the availability of captions on these services (see Ellcessor, Ellis and Kent). Netflix, as the current leading provider of subscription video entertainment in both the US and with a large market shares in other countries, has been at the centre of these discussions. While Netflix offers a comprehensive range of captioned video on its service today, there are still videos that do not have captions, particularly in non-English regions. As a result, users have endeavoured to produce user-generated captions for personal use and to find workarounds to access these through the Netflix system. This has been achieved with some success.There are a number of ways in which captions or subtitles can be added to Netflix video content to improve its accessibility for individual users. An early guide in a 2011 blog post (Emil’s Celebrations) identified that when using the Netflix player using the Silverlight plug-in, it is possible to access a hidden menu which allows a subtitle file in the DFXP format to be uploaded to Netflix for playback. However, this does not appear to provide this file to all Netflix users, and is generally referred to as a “soft upload” just for the individual user. Another method to do this, generally credited as the “easiest” way, is to find a SRT file that already exists for the video title, edit the timing to line up with Netflix, use a third-party tool to convert it to the DFXP format, and then upload it using the hidden menu that requires a specific keyboard command to access. While this may be considered uncomplicated for some, there is still a certain amount of technical knowledge required to complete this action, and it is likely to be too complex for many users.However, constant developments in technology are assisting with making access to captions an easier process. Recently, Cosmin Vasile highlighted that the ability to add captions and subtitle tracks can still be uploaded providing that the older Silverlight plug-in is used for playback instead of the new HTML5 player. Others add that it is technically possible to access the hidden feature in an HTML5 player, but an additional Super Netflix browser plug-in is required (Sommergirl). Further, while the procedure for uploading the file remains similar to the approach discussed earlier, there are some additional tools available online such as Subflicks which can provide a simple online conversion of the more common SRT file format to the DFXP format (Subflicks). However, while the ability to use a personal caption or subtitle file remains, the most common way to watch Netflix videos with alternative caption or subtitle files is through the use of the Smartflix service (Smartflix). Unlike other ad-hoc solutions, this service provides a simplified mechanism to bring alternative caption files to Netflix. The Smartflix website states that the service “automatically downloads and displays subtitles in your language for all titles using the largest online subtitles database.”This automatic download and sharing of captions online—known as fansubbing—facilitates easy access for all. For example, blog posts suggest that technology such as this creates important access opportunities for people who are deaf and hard of hearing. Nevertheless, they can be met with suspicion by copyright holders. For example, a recent case in the Netherlands ruled fansubbers were engaging in illegal activities and were encouraging people to download pirated videos. While the fansubbers, like the hackers discussed earlier, argued they were acting in the greater good, the Dutch antipiracy association (BREIN) maintained that subtitles are mainly used by people downloading pirated media and sought to outlaw the manufacture and distribution of third party captions (Anthony). The fansubbers took the issue to court in order to seek clarity about whether copyright holders can reserve exclusive rights to create and distribute subtitles. However, in a ruling against the fansubbers, the court agreed with BREIN that fansubbing violated copyright and incited piracy. What impact this ruling will have on the practice of user-generated captioning online, particularly around popular sites such as Netflix, is hard to predict; however, for people with disabilities who were relying on fansubbing to access content, it is of significant concern that the contention that the main users of user-generated subtitles (or captions) are engaging in illegal activities was so readily accepted.ConclusionThis article has focused on user-generated captions and the types of platforms available to create these. It has shown that this desire to provide access, to set the information free, has resulted in the disability digerati finding workarounds to allow users to upload their own captions and make content accessible. Indeed, the Internet and then the Web as a place for information sharing is evident throughout this history of user-generated captioning online, from Berner-Lee’s conception of community captioning, to Emil and Vasile’s instructions to a Netflix community of captioners, to finally a group of fansubbers who took BRIEN to court and lost. Therefore, while we have conceived of the disability digerati as a conflation of the hacker and the acknowledged digital influencer, these two positions may again part ways, and the disability digerati may—like the hackers before them—be driven underground.Captioned entertainment content offers a powerful, even vital, mode of inclusion for people who are deaf or hard of hearing. Yet, despite Berners-Lee’s urging that everything online be made accessible to people with all sorts of disabilities, captions were not addressed in the first iteration of the WCAG, perhaps reflecting the limitations of the speed of the medium itself. This continues to be the case today—although it is no longer difficult to stream video online, and Netflix have reached global dominance, audiences who require captions still find themselves fighting for access. Thus, in this sense, user-generated captions remain an important—yet seemingly technologically and legislatively complicated—avenue for inclusion.ReferencesAnthony, Sebastian. “Fan-Made Subtitles for TV Shows and Movies Are Illegal, Court Rules.” Arstechnica UK (2017). 21 May 2017 <https://arstechnica.com/tech-policy/2017/04/fan-made-subtitles-for-tv-shows-and-movies-are-illegal/>.Amara. “Amara Makes Video Globally Accessible.” Amara (2010). 25 Apr. 2017. <https://amara.org/en/ 2010>.Berners-Lee, Tim. “World Wide Web Consortium (W3C) Launches International Web Accessibility Initiative.” Web Accessibility Initiative (WAI) (1997). 19 June 2010. <http://www.w3.org/Press/WAI-Launch.html>.Bush, Vannevar. “As We May Think.” The Atlantic (1945). 26 June 2010 <http://www.theatlantic.com/magazine/print/1969/12/as-we-may-think/3881/>.CNET. “YouTube Turns 10: The Video Site That Went Viral.” CNET (2015). 24 Apr. 2017 <https://www.cnet.com/news/youtube-turns-10-the-video-site-that-went-viral/>.Downey, Greg. 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Ed. Ronald Berger. Boulder, London: Lynne Rienner Publishers, 2012.Levey, Steven. Hackers: Heroes of the Computer Revolution. North Sebastopol: O’Teilly Media, 1984.Media Access Australia. “How to Caption a Youtube Video.” 2017. 25 Apr. 2017 <https://mediaaccess.org.au/web/how-to-caption-a-youtube-video>.New Media Rock Stars. “Youtube’s 5 Worst Hilariously Catastrophic Auto Caption Fails.” 2013. 25 Apr. 2017 <http://newmediarockstars.com/2013/05/youtubes-5-worst-hilariously-catastrophic-auto-caption-fails/>.Outlaw. “Berners-Lee Applies Web 2.0 to Improve Accessibility.” Outlaw News (2006). 25 June 2010 <http://www.out-law.com/page-6946>.Raymond, Eric S. The New Hacker’s Dictionary. 3rd ed. Cambridge: MIT P, 1996.Smartflix. “Smartflix: Supercharge Your Netflix.” 2017. 9 Apr. 2017 <https://www.smartflix.io/>.Sommergirl. “[All] Adding Subtitles in a Different Language?” 2016. 9 Apr. 2017 <https://www.reddit.com/r/netflix/comments/32l8ob/all_adding_subtitles_in_a_different_language/>.Subflicks. “Subflicks V2.0.0.” 2017. 9 Apr. 2017 <http://subflicks.com/>.Vasile, Cosmin. “Netflix Has Just Informed Us That Its Movie Streaming Service Is Now Available in Just About Every Country That Matters Financially, Aside from China, of Course.” 2016. 9 Apr. 2017 <http://news.softpedia.com/news/how-to-add-custom-subtitles-to-netflix-498579.shtml>.Vogelstein, Fred. “The Wired Interview: Facebook’s Mark Zuckerberg.” Wired Magazine (2009). 20 Jun. 2010 <http://www.wired.com/epicenter/2009/06/mark-zuckerberg-speaks/>.W3C. “Web Content Accessibility Guidelines 1.0.” W3C Recommendation (1999). 25 Jun. 2010 <http://www.w3.org/TR/WCAG10/>.———. “Web Content Accessibility Guidelines (WCAG) 2.0.” 11 Dec. 2008. 21 Aug. 2013 <http://www.w3.org/TR/WCAG20/>.WGBH. “Magpie 2.0—Free, Do-It-Yourself Access Authoring Tool for Digital Multimedia Released by WGBH.” 2002. 25 Apr. 2017 <http://ncam.wgbh.org/about/news/pr_05072002>.YouTube. “Finally, Caption Video Playback.” 2006. 24 Apr. 2017 <http://googlevideo.blogspot.com.au/2006/09/finally-caption-playback.html>.

In the contemporary urban environment of mass transit, it falls to a small group of public officers to keep large number of travellers safe. The small size of their force and the often limited powers they exert mean that these public safety ‘transit officers’ must project more authority and control than they really have. It is this ambience of authority and control which, in most situations they encounter and seek to influence, is enough to keep the public safe. This paper examines the ambience of a group of transit officers working on the railway lines of an Australian capital city. We seek to show how transit officers are both influenced by, and seek to influence, the ambience of their workplace and the public spaces they inhabit whilst on duty, and here we take ambience to apply to the surrounding atmosphere, the aura, and the emotional environment of a place or situation: the setting, tone, or mood. For these transit officers to keep the public safe, they must themselves remain safe. A transit officer who is disabled in a confrontation with a violent offender is unable to provide protection to his or her passengers. Thus, in the culture of the transit officers, their own workplace safety takes on a higher significance. It affects not just themselves. The ambience exuded by transit officers, and how transit officers see their relationship with the travelling public, their management and other organisational work groups, is an important determinant of their work group’s safety culture. Researching the Working Lives of Transit Officers in Perth Our discussion draws on an ethnographic study of the working lives and communication cultures of transit officers (TOs) employed by the Public Transport Authority (PTA) of Western Australia (WA). Transit officers have argued that to understand fully the challenges of their work it is necessary to spend time with them as they undertake their daily duties: roster in, roster out. To this end, the research team and the employer organisation secured an ARC Linkage Grant in partnership with the PTA to fund doctoral candidate and ethnographer Christine Teague to research the workers’ point of view, and the workers’ experiences within the organisation. The two-hundred TOs are unique in the PTA. Neither of the other groups who ride with them on the trains, the drivers and revenue protection staff (whose sole job is to sell and check tickets), experiences the combination of intense contact with passengers, danger of physical injury or group morale. The TOs of the PTA in Perth operate from a central location at the main train station and the end stations on each line. Here there are change lockers where they can lock up their uniforms and equipment such as handcuffs and batons when not on duty, an equipment room where they sign out their radios, and ticket-checking machines. At the main train station there is also a gym, a canteen and holding cells for offenders they detain. From these end stations and central location, the TOs fan out across the network to all suburbs where they either operate from stations or onboard the trains. The TOs also do ‘delta van’ duty providing rapid, mobile back-up support for their colleagues on stations or trains, and providing transport for arrested persons to the holding cell or police lock up. TOs are on duty whenever the trains are running–but the evenings and nights are when they are mainly rostered on. This is when trouble mostly occurs. The TOs’ work ends only after the final train has completed its run and all offenders who may require detaining and charging have been transferred into police custody. While the public perceive that security is the TOs’ most frequent role, much of the work involves non-confrontational activity such as assisting passengers, checking tickets and providing a reassuring presence. One way to deal with an ambiguous role is to claim an ambience of power and authority regardless. Various aspects of the TO role permit and hinder this, and the paper goes on to consider aspects of ambience in terms of fear and force, order and safety, and role confusion. An Ambience of Fear and Force The TOs are responsible for front-line security in WA’s urban railway network. Their role is to offer a feeling of security for passengers using the rail network after the bustle of the work day finishes, and is replaced by the mainly recreational travels of the after hours public. This is the time when some passengers find the prospect of evening travel on the public transport rail network unsettling–so unsettling that it was a 2001 WA government election promise (WA Legislative Council) that every train leaving the city centre after 7pm would have two TOs riding on it. Interestingly, recruitment levels have never been high enough for this promise to be fully kept. The working conditions of the TOs reflect the perception, and to an extent, the reality that some late night travel on public transport involves negotiating an edgy ambience with an element of risk, rubbing shoulders with people who may be loud, rowdy, travelling in a group, and or drug and alcohol affected. As Fred (all TO names are pseudonyms) comments: You’re not dealing with rational people, you’re not dealing with ‘people’: most of the people you’re dealing with are either drunk or under the influence of drugs, so they’re not rational, they don’t hear you, they don’t understand what you’re saying, they just have no sense of what’s right or wrong, you know? Especially being under the influence, so I mean, you can talk till you’re blue in the face with somebody who’s drunk or on drugs, I mean, all you have to say is one thing. ‘Oh, can I see your ticket please’, ‘oh, why do I need a fucking ticket’, you know? They just don’t get simple everyday messages. Dealing with violence and making arrest is a normal part of this job. Jo described an early experience in her working life as a TO:Within the first week of coming out of course I got smacked on the side of the head, but this lady had actually been certified, like, she was nuts. She was completely mental and we were just standing on the train talking and I’ve turned around to say something to my partner and she was fine, she was as calm as, and I turned around and talked to my partner and the next thing I know I ended up with her fist to the side of my head. And I went ‘what the hell was that’? And she went off, she went absolutely ballistic. I ended up arresting her because it was assault on an officer whether she was mental or not so I ended up arresting her.Although Jo here is describing how she experienced an unprovoked assault in the early days of her career as a TO, one of the most frequent precursors to a TO injury occurs when the TO is required to make an arrest. The injury may occur when the passenger to be arrested resists or flees, and the TO gives chase in dark or treacherous circumstances such as railway reserves and tunnels, or when other passengers, maybe friends or family of the original person of concern, involve themselves in an affray around the precipitating action of the arrest. In circumstances where capsicum spray is the primary way of enforcing compliance, with batons used as a defence tool, group members may feel that they can take on the two TOs with impunity, certainly in the first instance. Even though there are security cameras on trains and in stations, and these can be cued to cover the threatening or difficult situations confronting TOs, the conflict is located in the here-and-now of the exchanges between TOs and the travelling public. This means the longer term consequence of trouble in the future may hold less sway with unruly travellers than the temptation to try to escape from trouble in the present. In discussing the impact of remote communications, Rubert Murdoch commented that these technologies are “a powerful influence for civilised behaviour. If you are arranging a massacre, it will be useless to shoot the cameraman who has so inconveniently appeared on the scene. His picture will already be safe in the studio five thousand miles away and his final image may hang you” (Shawcross 242). Unfortunately, whether public aggression in these circumstances is useless or not, the daily experience of TOs is that the presence of closed circuit television (CCTV) does not prevent attacks upon them: nor is it a guarantee of ‘civilised behaviour’. This is possibly because many of the more argumentative and angry members of the public are dis-inhibited by alcohol or other drugs. Police officers can employ the threat or actual application of stun guns to control situations in which they are outnumbered, but in the case of TOs they can remain outnumbered and vulnerable until reinforcements arrive. Such reinforcements are available, but the situation has to be managed through the communication of authority until the point where the train arrives at a ‘manned’ station, or the staff on the delta vehicle are able to support their colleagues. An Ambience of Order and Safety Some public transport organisations take this responsibility to sustain an ambience of order more seriously than others. The TO ethnographer, Christine Teague, visited public transport organisations in the UK, USA and Canada which are recognised as setting world-class standards for injury rates of their staff. In the USA particularly, there is a commitment to what is called ‘the broken windows’ theory, where a train is withdrawn from service promptly if it is damaged or defaced (Kelling and Coles; Maple and Mitchell). According to Henry (117): The ‘Broken Windows’ theory suggests that there is both a high correlation and a causal link between community disorder and more serious crime: when community disorder is permitted to flourish or when disorderly conditions or problems are left untended, they actually cause more serious crime. ‘Broken windows’ are a metaphor for community disorder which, as Wilson and Kelling (1982) use the term, includes the violation of informal social norms for public behaviour as well as quality of life offenses such as littering, graffiti, playing loud radios, aggressive panhandling, and vandalism.This theory implies that the physical ambience of the train, and by extension the station, may be highly influential in terms of creating a safe working environment. In this case of ‘no broken window’ organisations, the TO role is to maintain a high ‘quality of life’ rather than being a role predominantly about restraining and bringing to justice those whose behaviour is offensive, dangerous or illegal. The TOs in Perth achieve this through personal means such as taking pride in their uniforms, presenting a good-natured demeanour to passengers and assisting in maintaining the high standard of train interiors. Such a priority, and its link to reduced workforce injury, suggests that a perception of order impacts upon safety. It has long been argued that the safety culture of an organisation affects the safety performance of that organisation (Pidgeon; Leplat); but it has been more recently established that different cultural groupings in an organisation conceive and construct their safety culture differently (Leith). The research on ‘safety culture’ raises a problematic which is rarely addressed in practice. That problematic is this: managers frequently engage with safety at the level of instituting systems, while workers engage with safety in terms of behaviour. When Glendon and Litherland comment that, contrary to expectations, they could find no relationship between safety culture and safety performance, they were drawing attention to the fact that much managerial safety culture is premised upon systems involving tick boxes and the filling in of report forms. The broken window approach combines the managerial tick box with managerial behaviour: a dis-ordered train is removed from service. To some extent a general lack of fit between safety culture and safety performance endorses Everett’s view that it is conceptually inadequate to conceive organisations as cultures: “the conceptual inadequacy stems from the failure to distinguish between culture and behavioural features of organizational life” (238). The general focus upon safety culture as a way of promoting improvements in safety performance assumes that compliance with a range of safety systems will guarantee a safe workplace. Such an assumption, however, risks positioning the injured worker as responsible for his or her own predicament and sets up an environment in which some management officials are wont to seek ways in which that injured worker’s behaviour failed to conform with safety rules or safety processes. Yet there are roles which place workers in harm’s way, including military duties, law enforcement and some emergency services. Here, the work becomes dangerous as it becomes disorderly. An Ambience of Roles and Confusion As the research reported here progressed, it became clear that the ambience around the presentation of the self in the role of a TO (Goffman) was an important part of how ‘safety’ was promoted and enacted in their work upon the PTA (WA) trains, face to face with the travelling public. Goffman’s view of all people, not specifically TOs, is that: Regardless of the particular objective which the individual has in mind and of his motive for having this objective, it will be in his interests to control the conduct of the others, especially their responsive treatment of him. This will largely be through influencing the perception and definition that others will come to formulate of him. He will influence them by expressing himself in such a way that the kind of impression given off will lead them to act voluntarily in accordance with his own plan. (3)This ‘influencing of perception’ is an important element of performing the role of a TO. This task of the TOs is made all the more difficult because of confusions about their role in relation to two other officers: police (who have more power to act in situations of public safety) and revenue project officers (who have less), as we now discuss. The aura of the TO role borrows somewhat from those quintessential law and order officers: the police. TOs work in pairs, like many police, to support each other. They have a range of legal powers including the power of arrest, and they carry handcuffs, a baton and capsicum spray as a means of helping ensure their safety and effectiveness in circumstances where they might be outnumbered. The tools of their trade are accessibly displayed on heavy leather belts around their waists and their uniforms have similarities with police uniforms. However, in some ways these similarities are problematic, because TOs are not afforded the same respect as police. This situation underlines of the ambiguities negotiated within the ambience of what it is to be a TO, and how it is to conduct oneself in that role. Notwithstanding the TOs’ law and order responsibilities, public perceptions of the role and some of the public’s responses to the officers can position these workers as “plastic cops” (Teague and Leith). The penultimate deterrent of police officers, the stun gun (Taser), is not available to TOs who are expected to control all incidents arising on duty through the fact that they operate in pairs, with capsicum spray available and, as a last resort, are authorised to use their batons in self defence. Furthermore, although TOs are the key security and enforcement staff in the PTA workforce, and are managed separately from related staff roles, they believe that the clarity of this distinction is compromised because of similarities in the look of Revenue Protection Officers (RPOs). RPOs work on the trains to check that passengers have tickets and have paid the correct fares, and obtain names and addresses to issue infringement notices when required. They are not PTA employees, but contracted staff from an outside company. They also work in pairs. Significantly, the RPO uniform is in many respects identical to that of the TO, and this appears to be a deliberate management choice to make the number of TOs seem greater than it is: extending the TO ambience through to the activities of the RPOs. However, in the event of a disturbance, TOs are required and trained to act, while RPOs are instructed not to get involved; even though the RPOs appear to the travelling public to be operating in the role of a law-and-order-keeper, RPOs are specifically instructed not to get involved in breaches of the peace or disruptive passenger behaviour. From the point of view of the travelling public, who observe the RPO waiting for TOs to arrive, it may seems as if a TO is passively standing by while a chaotic situation unravels. As Angus commented: I’ve spoken to quite a few members of public and received complaints from them about transit officers and talking more about the incident have found out that it was actually [RPOs] that are dealing with it. So it’s creating a bad image for us …. It’s Transits that are copping all the flak for it … It is dangerous for us and it’s a lot of bad publicity for us. It’s hard enough, the job that we do and the lack of respect that we do get from people, we don’t need other people adding to it and making it harder. Indeed, it is not only the travelling public who can mistake the two uniforms. Mike tells of an “incident where an officer [TO] has called for backup on a train and the guys have got off [the train at the next station] and just stood there, and he didn’t realise that they are actually [revenue protection] officers, so he effectively had no backup. He thought he did, but he didn’t.” The RPO uniform may confer an ambience of power borrowed from TOs and communicated visually, but the impact is to compromise the authority of the TO role. Unfortunately, what could be a complementary role to the TOs becomes one which, in the minds of the TO workforce, serves to undermine their presence. This effect of this role confusion is to dilute the aura of authority of the TOs. At one end of a power continuum the TO role is minimised by those who see it as a second-rate ‘Wannabe cop’ (Teague and Leith 2008), while its impact is diluted at the other end by an apparently deliberate confusion between the TO broader ‘law and order’ role, and the more limited RPO revenue collection activities. Postlude To the passengers of the PTA in Perth, the presence and actions of transit officers appear as unremarkable as the daily commute. In this ethnographic study of their workplace culture, however, the transit officers have revealed ways in which they influence the ambience of the workplace and the public spaces they inhabit whilst on duty, and how they are influenced by it. While this ambient inter-relationship is not documented in the organisation’s occupational safety and health management system, the TOs are aware that it is a factor in their level at safety at work, both positively and negatively. Clearly, an ethnography study is conducted at a certain point in time and place, and culture is a living and changing expression of human interaction. The Public Transport Authority of Western Australia is committed to continuous improvement in safety and to the investigation of all ways and means in which to support TOs in their daily activities. This is evident not only in their support of the research and their welcoming of the ethnographer into the workforce and onto the tracks, but also in their robust commitment to change as the findings of the research have progressed. In particular, changes in the ambient TO culture and in the training and daily practices of TOs have already resulted from this research or are under active consideration. Nonetheless, this project is a cogent indicator of the fact that a safety culture is critically dependent upon intangible but nonetheless important factors such as the ambience of the workplace and the way in which officers are able to communicate their authority to others. References Everett, James. “Organizational Culture and Ethnoecology in Public Relations Theory and Practice.” Public Relations Research Annual. Vol. 2. Eds. Larissa Grunig and James Grunig. Hillsdale, NJ, 1990. 235-251. 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