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Journal articles on the topic 'Citrate Utilization test'

Author: Grafiati

Published: 5 June 2025

Last updated: 2 August 2025

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1

Anaizi, N. H., J. J. Cohen, A. J. Black, and S. J. Wertheim. "Renal tissue citrate: independence from citrate utilization, reabsorption, and pH." American Journal of Physiology-Renal Physiology 251, no. 3 (1986): F547—F561. http://dx.doi.org/10.1152/ajprenal.1986.251.3.f547.

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During alkalosis in vivo, renal tissue [citrate] [( citrate]t) increases and citrate reabsorption (Tcit) and utilization (Qcit) simultaneously decrease. The decrease in Qcit is interpreted to cause the increased [citrate]t, which in turn decreases Tcit X Renal citrate handling and [citrate]t could be regulated by other mechanisms, since alkalosis changes [substrate] and [H+] in extracellular (ECF) and intracellular (ICF) fluid. Also, since high plasma [citrate] decreases ionized [Ca2+] (Cai), it is not possible to determine in vivo whether there is a maximum for Tcit or Qcit and whether change

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2

Eruchalu I Augustine. "Identification of Proteus Vulgaris using Series of Biochemical Test and Staining Technique: Ecology, Pathogenicity (Enteric Pathogen), Description and Prevention of It’s Diseases." International Journal of Contemporary Microbiology 10, no. 1 (2024): 18–23. http://dx.doi.org/10.37506/c4fpes96.

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Phenotypic identification of bacteria by staining technique and biochemical tests. This practical was carried out to identify Proteus vulgaris using biochemical test, this test includes, citrate utilization, indole, carbohydrate fermentation, methyl red, voges Proskauer, motility, production of hydrogen sulphide, catalase, and oxidase tests. Results obtained shows that Proteus vulgaris was positive to indole, motility, methyl red and urease. Proteus vulgaris was unable to utilize citrate for carbon and energy, and does not synthesis the enzyme amylase to break down starch. The bacteria ferment

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RIZKY, VINCENTIA ADE, IKKE ELMALIA PUTRI, and SAADAH SIREGAR. "Utilization of Lemon Juice (Citrus limon L.) as an Alternative Substitute for Sodium Citrate in Blood Sedimentation Rate Examination." MEDISTRA MEDICAL JOURNAL (MMJ) 2, no. 1 (2024): 15–19. http://dx.doi.org/10.35451/mmj.v2i1.2370.

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Background: The erythrocyte sedimentation rate (LED) examination is a hematological test that describes the rate at which red blood cells settle in plasma, using 3.8% sodium citrate anticoagulant and is measured in mm/hour. Lemon (Citrus limon L.) is a plant with natural properties as a metal binding agent or chelating agent. Lemons contain vitamin C, citric acid, potassium, and limonene. Citric acid compounds are able to form complex bonds with metals because they have COOH and -OH functional groups. Apart from citric acid, lemons contain flavonoids which function as antiviral, anticancer and

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Mohsin, Umema, Madan Lowry, Pooja Devi, Himanshu Dixit, and Shaile Thakur. "Evaluation of Antibiotic Resistant and Metal Tolerances Capability of Petroleum (hydrocarbon) Degrading Bacteria." IOP Conference Series: Materials Science and Engineering 1224, no. 1 (2022): 012022. http://dx.doi.org/10.1088/1757-899x/1224/1/012022.

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Abstract Diesel oil is considered one of the important products of crude oil which constitute a vital source of pollution in the environment. Biodegradation of complex hydrocarbon requires cooperation of more than a single species. This is true in case of pollutants made up of crude oil or petroleum. Soil contains varieties of microorganism that can be established in any natural environment. The bacterial isolates were characterized biochemically by indole test, methyl red test, citrate utilization Test, voges proskauer test, starch hydrolysis, catalase production test, nitrate reduction test,

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Coggan, A. R., R. J. Spina, W. M. Kohrt, and J. O. Holloszy. "Effect of prolonged exercise on muscle citrate concentration before and after endurance training in men." American Journal of Physiology-Endocrinology and Metabolism 264, no. 2 (1993): E215—E220. http://dx.doi.org/10.1152/ajpendo.1993.264.2.e215.

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It has been hypothesized that endurance training reduces carbohydrate utilization during exercise via citrate-mediated inhibition of phosphofructokinase (PFK). To test this hypothesis, vastus lateralis muscle biopsy samples were obtained from eight men before and immediately (approximately 10 s) after 2 h of cycle ergometer exercise at 60% of pretraining peak O2 uptake, both before and after 12 wk of endurance exercise training (3 days/wk running, 3 days/wk interval cycling). Training increased muscle citrate synthase (CS) activity from 3.69 +/- 0.48 (SE) to 5.30 +/- 0.42 mol.h-1.kg protein-1

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Pria, S., and O. N. Shanmugapriya. "COMPARITIVE STUDY ON THE EFFECT OF RHOZOBIUM INOCULUM ON IMBEDDED SEEDS AND GERMINATED SEEDS OF VIGNO MUNGO L." JOURNAL OF ADVANCES IN NATURAL SCIENCES 3, no. 1 (2015): 174–77. http://dx.doi.org/10.24297/jns.v3i1.5011.

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The Rhizobium was isolated from the plant root and purified. The isolated rhizobium sample was identified based on morphological and bio chemical characterization. The result of biochemical tests showed positive result in indole test, citrate utilization test,starch hydrolysis test, urea hydrolysis test and catalase test. The isolated culture was mass multiplied and used by seed treatment like imbibed treatment and germinated treatment. After treatment the seedlings per planted separately by part culture method. The result showed that the rhizobium inoculation on the emerging radicals of one d

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7

Yanti, Nur Arfa. "Enhancement of Cocoa Quality By the Indigenous Yeast Candida Tropicalis KLK4 Through Cocoa Bean Fermentation." JOURNAL OF ADVANCES IN BIOTECHNOLOGY 4, no. 1 (2014): 327–35. http://dx.doi.org/10.24297/jbt.v4i1.1640.

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An indigenous yeast strain that isolated from fermented cocoa bean has a role in cocoa bean fermentation process, was characterized and identified to be member of the Candida tropicalis Â species Â based on phenotypic characteristics and the D1/D2 region of the large subunit rRNA gene. Properties of importance for cocoa bean fermentation, namely sucrose, glucose, and citrate assimilation capacity, pH-, ethanol-, and heat-tolerance, were examined for isolate. The Quality of fermented cocoa bean was analyzed by cut test. Candida tropicalis KLK4 was tolerance to low pH value, high temperature, et

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8

Caiazza, Nicky C., Douglas P. Lies, and Dianne K. Newman. "Phototrophic Fe(II) Oxidation Promotes Organic Carbon Acquisition by Rhodobacter capsulatus SB1003." Applied and Environmental Microbiology 73, no. 19 (2007): 6150–58. http://dx.doi.org/10.1128/aem.02830-06.

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ABSTRACT Anoxygenic phototrophic Fe(II) oxidation is usually considered to be a lithoautotrophic metabolism that contributes to primary production in Fe-based ecosystems. In this study, we employed Rhodobacter capsulatus SB1003 as a model organism to test the hypothesis that phototrophic Fe(II) oxidation can be coupled to organic carbon acquisition. R. capsulatus SB1003 oxidized Fe(II) under anoxic conditions in a light-dependent manner, but it failed to grow lithoautotrophically on soluble Fe(II). When the strain was provided with Fe(II)-citrate, however, growth was observed that was dependen

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S.L, Gbarakoro, Okorosaye-Orubite K, and Amadi K. "Characterization of Bacteria Isolates from Surfaces of Corroded Zinc and Copper Coupons." INTERNATIONAL JOURNAL OF CHEMISTRY AND CHEMICAL PROCESSES 8, no. 1 (2022): 19–27. http://dx.doi.org/10.56201/ijccp.v8.no1.2022.pg19.27.

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This research characterized bacteria isolates from surfaces of corroded zinc and copper coupons. Corroded zinc and copper materials were collected carefully, from two different locations in Ken Saro-Wiwa Polytechnic Campus and were put in sterile McCartney bottles. Bacteriological analysis such as Gram staining, motility test, catalase test, indole test, oxidase test, methyl red test, Voges-Proskauer test, and citrate utilization test were carried out t o ascertain the bacteria responsible for the corrosion. Two bacteria were isolated; Enterococcus which was seen to be responsible for the corr

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R, Nirmalkumar, Uma S., and Indumathi Parameswaran. "Isolation and Characterization of Bio-surfactant Producing Bacteria." International Journal of Zoological Investigations 08, special issue (2022): 126–35. http://dx.doi.org/10.33745/ijzi.2022.v08i0s.016.

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Oil spillages are so common in many fields which lead to contaminated soil. Soil polluted land filling contains bacteria that produce a broad spectrum of bio-surfactants. Bio-surfactant as an efficient biological surfaceactive agent may provide an alternative solution for the removal of heavy metals from industrial wastes. The biosurfactant has more potential efficiency in the process of remedying natural resources. Surfactin, Iturin and Fengycin are widely studied bio-surfactants. The present study was aimed to isolate and characterize the biosurfactant producing bacteria from the soil sample

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M, Syamala, and Yesu Raja I. "Physiological and Biochemical Variations in Soft Rot Pathogen (Pectobacterium chrysanthemi) Isolates of Aloe vera." Madras Agricultural Journal 99, JUNE (2012): 338–43. http://dx.doi.org/10.29321/maj.10.100081.

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All the 15 isolates of Pectobacterium chrysanthemi survived up to 37 0 C and the thermal death point was 38 0 C. The optimum range of pH for the growth of the isolates was 7.0–7.5.Although all the isolates showed good growth in many carbon sources, salicin supported the maximum growth while ribose was the least favourable. These isolates showed no differences in all the biochemical tests. All the 15 isolates of P. chrysanthemi gave positive result to utilization of citrate, nitrate reductase test, catalase test, Oxidation-Fermentation test (O- F test), Gelatin liquefaction, casein hydrolysis,

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S, Yaswanthkumar, Raja S, Pavithra R, Kannan K, and Navaneethan Muthu Krishnan Muthu Krishnan. "Isolation, estimation of bacterial load, and characterization of gut microbes of three Garra species from Bhavani river, Western ghats,Tamil Nadu, India." International Journal of Zoology and Applied Biosciences 8, no. 3 (2023): 27–31. http://dx.doi.org/10.55126/ijzab.2023.v08.i03.005.

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Microbiomes play an important role in fish physiology, particularly in digestion, by metabolizing largely indigestible feed components for the host or synthesis of essential micronutrients. The gut flora has a dynamic effect on the host’s gut and systemic immunity. This study is the preliminary exploration of gut microflora of three species Garra mcclellandi, Garra stenorhynchus, and Garra gotyla stenorhynchus . The total bacterial load ranges from 66×108 CFU/g to 81×108 CFU/g in the gut sample. It was found that the maximum load in G. stenorhynchus with 81×108 CFU/g followed by Gg. stenorhync

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ZOU, ZIYING, YUAN LIU, BING ZHU, and PING ZENG. "Direct identification of Mycobacterium abscessus through 16S rDNA sequence analysis and a citrate utilization test: A case report." Experimental and Therapeutic Medicine 8, no. 1 (2014): 115–17. http://dx.doi.org/10.3892/etm.2014.1705.

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14

Choi, Yunju, Sun-Young Lee, Jong-Seong Bae, et al. "Nitrogen and Sulfur Co-Doped Porous Carbon Derived from Thiourea and Calcium Citrate for Lithium-Sulfur Batteries." Applied Sciences 10, no. 4 (2020): 1263. http://dx.doi.org/10.3390/app10041263.

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Lithium-sulfur (Li-S) batteries have shown a high theoretical specific capacity of 1675 mAh g−1. However, amongst the issues they have, the low electron conductivity of sulfur and its dissolution represent the biggest challenge limiting its practical applications. This contributes to the low utilization of the active sulfur at the cathode—a phenomenon known as the “shuttling effect.” To overcome these limitations, some strategies such as physical confinement (sulfur–carbon composite), chemical adsorption (N and/or S doping) electrolyte design, and separator design have already been proposed. C

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15

Theng, A. D., R. M. Gade, P. R. Padghan, and Priya C. Atram. "BIOCHEMICAL AND MORPHOLOGICAL CHARACTERIZATION OF PSEUDOMONAS FLUORESCENS ISOLATES AND ITS EFFICACY AGAINST DAMPING-OFF IN CHILLI." Journal of Plant Disease Sciences 19, no. 2 (2024): 128–33. https://doi.org/10.48165/jpds.2024.19.02.07.

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This study examines three isolates of Pseudomonas fluorescens (PDKV Pf1, Pf2, Pf3) obtained from the chilli rhizosphere, focusing on their biochemical and morphological characteristics and their efficacy in suppressing Pythium aphanidermatum, the causal agent of damping-off in chilli. The isolates were evaluated for their ability to produce hydrogen cyanide (HCN) and indole-3-acetic acid (IAA), essential plant growth-promoting compounds. Morphologically, all isolates were identified as gram-negative and rod-shaped. Among them, PDKV Pf1 displayed the strongest biochemical activity, testing posi

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J. Lakshmi, Sirisha, and K. Lakshmi A. Vijaya Gopal. "Isolation and Biochemical Characterization of Lactic Acid Bacteria from Fermented Foods." International Journal of Current Microbiology and Applied Sciences 10, no. 10 (2021): 584–600. http://dx.doi.org/10.20546/ijcmas.2021.1010.069.

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Probiotics are considered as successful major category of food supplements. Probiotics can be functional foods because their health benefits are essentially higher than traditional nutritional products. Probiotic bacteria was collected from home made and commercial fermented food samples. A total of 30 food samples were collected from local areas of Guntur in Andhra Pradesh. Bacteria were isolated on MRS agar medium after observation of growth and pure culture was obtained by sub-culturing on the same medium. Purity of each culture was confirmed by morphological investigation, Gram’s staining

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Bose, Sanchali, Senthil Kumar Ponnusamy, and Pooja G. "A Preliminary Study on Detection of Serogenic E.coli in Fishes to Conduct a Microbial Assessment of Coastal Water." ECS Transactions 107, no. 1 (2022): 3863–73. http://dx.doi.org/10.1149/10701.3863ecst.

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Escherichia coli is a bacterial pathogen mostly responsible for gut infections and related serious illnesses. There have been some studies in the past which detected the presence of E.coli in edible fishes. The diarrheagenic E.coli strains are mainly responsible for such health issues. E.coli is an indicator of the microbiological quality of water as it indicates faecal contamination. In this paper, it is intended to understand the microbiological quality of coastal water inhabited by fish. The edible fishes consumed along the Vishakhapatnam coast were subjected to various tests like Indole pr

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18

Snozek, Christine L. H., James S. Hernandez, and Stephen J. Traub. "“Rainbow Draws” in the Emergency Department: Clinical Utility and Staff Perceptions." Journal of Applied Laboratory Medicine 4, no. 2 (2019): 229–34. http://dx.doi.org/10.1373/jalm.2018.027649.

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Abstract Background Collecting a predefined set of blood tubes (the “rainbow draw”) is a common but controversial practice in many emergency departments (EDs), with limited data to support it. We determined the actual utilization of rainbow draw tubes at a single facility and evaluated the perceptions of ED staff regarding the utility of rainbow draws. Methods We analyzed 2 weeks of ED visits (1326 visits by 1240 unique patients) to determine blood tube utilization for initial and add-on testing, as well as the incidence of additional venipunctures. We also surveyed ED staff regarding aspects

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19

Babu, S. Vinod, A. Vijaya Gopal, N. Trimurtulu, G. Kishore Babu, and S. L. Bhattiprolu. "Efficacy of Silica Solubilizing Bacteria as Plant Growth Promoting Rhizobacteria and Their Biochemical Characteristics." International Journal of Environment and Climate Change 14, no. 6 (2024): 199–210. http://dx.doi.org/10.9734/ijecc/2024/v14i64221.

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Twenty eight isolates of silica solubilising bacterial inoculants were collected from rhizosphere soils of rice crop in Kurnool, Prakasam, Guntur and Anantapur districts of Andhra Pradesh by using insoluble source of silica. These isolates were verified for plant growth promoting rhizobacteria (PGPR) activity and then subjected biochemical tests in the Department of Agricultural microbiology, Advanced Post Graduate Centre, ANGRAU, Lam, Guntur, Andhra Pradesh, India. Two isolates, SiKPP-1 (Silica solubilizing isolate from Kurnool district, Pamulapadu Mandal and Pamulapadu Village - 1) & SiP

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Sija, S. L., S. Reshmi, and T. S. Athira. "Isolation and molecular identification of actinomycetes from sacred grove soil." International Journal of Agricultural Sciences and Veterinary Medicine 12, no. 2 (2024): 10–19. https://doi.org/10.25303/1202ijasvm10019.

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Actinomycetes, a group of prokaryotes sharing traits with both bacteria and fungi, hold significant economic and commercial importance. They are prolific producers of enzymes, antibiotics, enzyme inhibitors and pharmaceuticals of high commercial value. This study aimed to characterize actinomycetes isolated from sacred groves, focusing on their morphological, biochemical and molecular traits and to assess their bioactive potential. Actinomycetes were isolated using plating and serial dilution methods followed by characterization through Gram staining and biochemical tests such as IMViC test (I

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Khan, Sonam, Nikita Negi, Anshika Pal, et al. "Preliminary isolation and biochemical characterization of plant growth promoting rhizobacteria from Curcuma longa from the high-altitude region of Uttarakhand." Environment Conservation Journal 26, no. 1 (2025): 45–51. https://doi.org/10.36953/ecj.30193154.

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Curcuma longa L. (common name: turmeric) is a medicinal herb with a rhizome and belongs to the Zingiberaceae family. The Indian subcontinent widely cultivates it, making it a significant commercial crop. Plant growth-promoting rhizobacteria (PGPR), which are found in the rhizosphere of plants, significantly contribute to plant growth. They aid in the mobilization and absorption of crucial nutrients like nitrogen, phosphorus, potassium, zinc, and others. They also contribute to the production of phytohormones by enhancing the microbial load, regulating both primary and secondary metabolite path

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Weli, Ikbal kh. "Biochemical and Molecular Detection of Aeromonas Hydrophila Isolated from Infected Cyprinus Carpio in Salah Al-Din Governorate." Wasit Journal for Pure sciences 3, no. 3 (2024): 241–51. http://dx.doi.org/10.31185/wjps.503.

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This study aimed to identify the presence of Aeromonas hydrophila bacteria in diseased common carp (Cyprinus carpio) fish collected from Salah al-Din Governorate in Iraq. For this purpose, 128 swabs were obtained from skin, gill and fin lesions of 70 fish showing symptoms of hemorrhagic sepsis, skin ulcers and boils, and fin and gill rot. Through different biochemical tests, the isolates showed negative results for Gram stain, methyl red, lactose fermentation, H2S, and string test, while positive results were recorded for glucose fermentation, oxidase, motility test, catalase, Voges-Proskauer,

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23

Borkar, R. D., R. M. Gade, R. J. Choudhari, A. V. Chinche, and N. V. Gurav. "Morphological, Physiological and Biohchemical Identification of Nitrogen Fixing Mesorhizobium in Chickpea." Journal of Plant Disease Sciences 18, no. 1 (2023): 59–62. http://dx.doi.org/10.48165/jpds.2023.1801.13.

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Chickpea is the world’s second most important legume, and it can be nodulated with a variety of rhizobial species to generate root nodules. The current study investigated the isolation, cultural, and biochemical characterization of several strains of root nodulating bacteria from different locations of Yavatmal districts. The serial dilution approach was used for isolation. Bacterium Mesorhizobium is a gram- negative, aerobic, non-spore producing, motile rods with circular, convex, whitish pink, and glistering colonies with an entire edge. The biochemical characterization of five different str

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Raouf, Tara Fouad Raouf, Melda DÖlarslan, and Rana Mujahid Abdul AL- Shwaikh. "Gene expression of psID gene in Pseudomonas aeruginosa isolated from clinical causes." Research Journal of Biotechnology 19, no. 11 (2024): 108–12. http://dx.doi.org/10.25303/1911rjbt1080112.

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One hundred clinical samples were collected from Baghdad hospitals including Teaching Laboratories Hospital, Medical City, Baghdad Teaching Hospital and Ghazi Hariri Hospital including burn infections, wound infections, urinary tract infections, otitis media from May 2022 to September 2022, The samples were identefied by microscopic, phenotypic and culturale on MacConkey agar and by biochemical tests including oxidase, catalase, citrate utilization test, triple sugar-iron test (TSI), gelatinase test, motility test and ornithine decarboxylation test. After diagnosis, we received 19 isolates of

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Khalid, Ibrahim yahaya, and Ibrahim Abdullahi Ahmad. "Isolation and identification of iron oxidizing bacteria from different sources of water within Kano metropolitan." International Journal of Scientific Development and Research 7, no. 12 (2022): 423–26. https://doi.org/10.5281/zenodo.11213766.

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Iron oxidizing bacteria were isolated on winogradsky medium from samples of tap water, iron reservoir, wells, borehole and scratched rusted water-borne pipe collected in Gwale Local Government, Ungogo Local Government and Kano Munincipal, Kano State, Nigeria. The bacterial viable count was ranged from  to  in Gwale while ungogo ranged from  to . Also for Kano Munincipal ranged from  to The bacterial isolates were all Gram negative bacilli observed microscopically. The apropriate biochemical test such as indole, methyl red (MR), citrate utilization, vogues proskauer (VP), ca

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YUSUF, Hajara Oyiza, Mercy MALLAM-DAUDA, Monica Ukungakpe AGAKWU, Idiongobong Emmanuel ASUQUO, Victoria Chinasa ELEBE, and Hauwa Shabu IBRAHIM. "Analysis of the microbial contamination in available cosmetic products from Samaru Market, Zaria, Kaduna State, Nigeria." Journal of Biotechnology 2, no. 1 (2024): 64–71. http://dx.doi.org/10.36108/jbt/3202.20.0150.

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The purpose of this investigation was to assess the level of microbial contamination of cosmetic goods found in Samaru Market, Zaria, Kaduna State, Nigeria. Ten samples of two distinct cosmetics: face powder (labeled as FP) and kohl (labeled as K); were obtained from merchants at Samaru Market. The samples were cultivated on nutrient agar and mannitol salt for the purposes of viable cell count, isolation, and microscopic identification. To further characterize the isolates from the agar, gram staining technique and biochemical tests (catalase, urease, indole, Voges-Proskauer, citrate utilizati

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Beylot, M., H. Vidal, G. Mithieux, M. Odeon, and C. Martin. "Inhibition of hepatic ketogenesis by tumor necrosis factor-alpha in rats." American Journal of Physiology-Endocrinology and Metabolism 263, no. 5 (1992): E897—E902. http://dx.doi.org/10.1152/ajpendo.1992.263.5.e897.

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Tumor necrosis factor-alpha (TNF-alpha) stimulates hepatic lipogenesis. Therefore, it could play a role in the control of ketogenesis. To test this hypothesis, we measured simultaneously free fatty acids (FFA; [1–13C]palmitate) and ketone body (KB; [3,4–13C2]acetoacetate) kinetics, before and after intraperitoneal injection of saline or TNF-alpha, in postabsorptive rats or rats starved for 24 h. In both groups of rats, TNF-alpha injection did not modify insulinemia and induced a moderate increase of FFA concentrations and appearance rates (P < 0.05). Despite increased FFA availability, keto

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Sawant, Shraddha Bhaskar, Mihira Kumara Mishra, SR Prabhukarthikeyan, Akshya Kumar Senapati, and Kailash Chandra Samal. "Biochemical characterization of Bacillus species isolated from rice rhizosphere in Odisha." Oryza-An International Journal on Rice 59, no. 4 (2022): 470–82. http://dx.doi.org/10.35709/ory.2022.59.4.10.

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The Plant growth-promoting rhizobacteria (PGPR) such as Bacillus spp. promotes plant growth directly or indirectly. The aim of this study was to isolate the Bacillus species from rice rhizosphere and characterize by biochemical methods. A total of 10 effective Bacillus spp. were identified using both classical and biochemical identification kit methods. The HiBacillusTM kit included a number of biochemical tests viz., citrate utilisation, malonate, voges proskauer’s, ONPG, nitrate reduction, catalase, arginine, sucrose, mannitol, glucose, arabinose and trehalose. The basis for the biochemical

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Singh, Shila K., Lahunlyne Kaharakor, and Najmiara S. Ahmed. "Rising Trend of Colistin Resistance and Shift in Minimum Inhibitory Concentrations Toward the Higher Side Among Enterobacteriaceae Isolated from Clinical Samples." Medical Journal of Dr. D.Y. Patil Vidyapeeth 18, no. 2 (2025): 282–87. https://doi.org/10.4103/mjdrdypu.mjdrdypu_295_24.

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ABSTRACT Background: The emergence of multidrug resistance (MDR) bacteria is a major global health issue that arises a concern for humanity especially when fewer options for antibiotics are available. Colistin used indiscriminately against gram-negative bacteria has led to a rise in Colistin resistance. In our study, we tried to find out the prevalence of Colistin-resistant Enterobacteriaceae isolates from outpatient/Inpatient departments (OPD/IPD) of a Tertiary care hospital. Methodology: 162 clinical samples were collected and processed by using different microbiological techniques gram stai

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Li, Hongmei, Huimin Huang, Ying Jia, Yuwei Tong, and Zhijun Zhou. "The Gut Bacteria of Gampsocleis gratiosa (Orthoptera: Tettigoniidae) by Culturomics." Insects 16, no. 2 (2025): 123. https://doi.org/10.3390/insects16020123.

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Gampsocleis gratiosa Brunner von Wattenwyl, 1862, is a type of omnivorous chirping insect with a long history of artificial breeding. It has high economic value and is also an excellent orthopteran model organism. In this study, 12 types of culture media combined with 16S rRNA sequencing were employed to isolate 838 bacterial strains from the gut of G. gratiosa. After sequence comparison, a total of 98 species of bacteria were identified, belonging to 3 phyla, 5 classes, 11 orders, 20 families, and 45 genera. Firmicutes and Proteobacteria accounted for the majority (92.86%). At the order level

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Anelechi, Madubuike Stella, Mailafia Samuel Egwu O. Godwin, Olabode H. Olatunde, Momoh A.Habiba, Ode O. Julius, and Ramon-Yusuf S.Babatunde. "Phenotypic Identification of Citrobacter Isolates from Cloacal Swabs of Apparently Healthy Turtles at the River Banks in Lokoja, Kogi State, Nigeria." International Journal of Current Microbiology and Applied Sciences 11, no. 11 (2022): 117–25. http://dx.doi.org/10.20546/ijcmas.2022.1111.014.

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Citrobacter species are opportunistic pathogens in humans that can lead to invasive disease, including infections of the urinary tract, respiratory tract, Central Nervous System, skin, and soft tissues. A total of 245 cloacal swab samples were collected from the cloaca of apparently healthy turtles and subjected to conventional biochemical tests. Presumptive identification on MacConkey Agar (MAC) and Xylose lysine deoxycholate agar (XLD) yielded 42.0% (103) on each medium, on Eosin Methylene Blue agar (EMB) yielded 21.6 % (53), and on Salmonella-Shigella agar yielded 9.8% (24) isolates while I

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Khdeer, A. Q. S., and R. N. Gergees. "Genetic diagnosis of phytopathogenic Agrobacterium tumefaciens isolates from Cydonia oblonga trees by PCR using 16SrRNA primers." Regulatory Mechanisms in Biosystems 33, no. 1 (2025): e25005. https://doi.org/10.15421/0225005.

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Agrobacterium is a plant pathogen causing crown disease and plant tumors in 140 or more dicotyledonous plant species. T u mor-causing agrobacteri a are members of the Rhizobiaceae family of nitrogen-fixing legume symbionts, but are harmful to plants. This study's initial goal was to isolate 25 bacterial isolates from crown tumors that developed on the stems of quince trees in various areas of Mosul. The colonies were selected for tests on the basis of the ir size, shape and color . From these , Gram-negative bacterial isolates were chosen and subjected to the following tests ; catalase, L-tyro

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Sidra, Gill. "Agrobacterium tumefaciens pathogenicity and characterization in rose crown gall disease." International Journal of Biosciences (IJB) 24, no. 4 (2024): 129–35. https://doi.org/10.12692/ijb/24.4.129-135.

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<em>Agrobacterium </em>is a bacterium that has a gigantic role in modern plant genetic engineering. As its beneficial role of the bacterium, it also causes crown gall diseases in many horticultural crops and causes losses all over the world. A crown gall-infected rose sample was collected and morphological, biochemical, and cultural characteristics using NA, YDC, YPDA and King’s B mediums. Different tests were conducted to study the biochemical properties of the bacterium such as gram staining, KOH solubility test, Starch hydrolysis, Kovac’s oxidase, Litmus milk, Hydrolysis of

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Saimin, Juminten, Hartati Hartati, Yenti Purnamasari, Sufiah Asri Mulyawati, Tien Tien, and Pranita Ayitrina. "Microbiological and Biochemical Contamination Analysis of Refilled Drinking-water in Abeli, Kendari, Southeast Sulawesi." Indonesian Biomedical Journal 12, no. 2 (2020): 124–9. http://dx.doi.org/10.18585/inabj.v12i2.871.

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BACKGROUND: The access to safe drinkingwater is increasingly difficult, especially in water catchment or coastal areas. Due to the difficulty, people in coastal areas tend to consume ready-to-use refilled drinking-water. However, the sanitation facilities on most drinking-water refill depots do not meet the requirements, hence really prone to microbial contamination. We conducted this study to determine the types of bacteria causing the contamination of refilled drinking-water from refill depots in Abeli, a coastal area in Kendari.METHODS: Samples were collected from all 6 drinkingwater refill

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Dameanti, Fidi Nur Aini Eka Puji, Jacky T. Amanda F, Muhammad Ali A. Safri, and Chyntia S. Y. Hasan. "Extended-spectrum beta-laktamase (ESBL) enzyme producing bacteria isolated from rectal swabs of around animals in dairy farms." Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) 14, no. 3 (2024): 119–24. http://dx.doi.org/10.46549/jipvet.v14i3.421.

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This study aims to identify bacterial contamination of Klebsiella pneumoniae and Escherichia coli producing Extended Spectrum Beta Lactamase (ESBL) isolated from rectal swabs of local animals on a dairy farm in Deyeng Village, Kediri, East Java. Interviews and observations were conducted with farmers to obtain the required risk factor data (cleanliness of drinking and eating places, drinking water sources, and administration of antibiotics to livestock). The number of samples tested in this study was 34 samples, including 12 chicken samples, 8 goat samples, 5 duck samples, 4 rabbit samples, 3

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Pervin, Safirun, Bushra Jannat, and Sohana Al Sanjee. "Characterization of Rhizobia from Root Nodule and Rhizosphere of Lablab purpureus and Vigna sinensis in Bangladesh." Turkish Journal of Agriculture - Food Science and Technology 5, no. 1 (2017): 14. http://dx.doi.org/10.24925/turjaf.v5i1.14-17.743.

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Nitrogen fixation resulting from mutual symbiosis of rhizobia and cultivated legume plants is therefore critical to food security as it directly affects agricultural production. Biological Nitrogen Fixation (BNF) can be an important factor in sustainable agriculture.The isolation and identification of different slow growing and fast growing rhizobial strains from the nodules of two leguminous plant species. Symbiotic nitrogen fixing Rhizobium spp. was isolated from (Lablab purpureus and Vigna sinensis). Nodules samples were collected from plants growing in different Districts of Bangladesh and

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Yesigat, Tibebu, Mohabaw Jemal, and Wubet Birhan. "Prevalence and Associated Risk Factors of Salmonella, Shigella, and Intestinal Parasites among Food Handlers in Motta Town, North West Ethiopia." Canadian Journal of Infectious Diseases and Medical Microbiology 2020 (January 13, 2020): 1–11. http://dx.doi.org/10.1155/2020/6425946.

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Background. Intestinal parasite, Salmonella, and Shigella infections are the main public health concerns in the world, especially in developing countries due to inaccessibility of safe water and unhygienic food handling practices of food handlers. Methods. A cross-sectional study was conducted in food handlers in Motta town, from February 2019 to April 2019. The study was undertaken to determine the prevalence and associated risk factors of Salmonella, Shigella, and intestinal parasites among 243 food handlers. Sociodemographic and risk factors were collected using pretested structured questio

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Ram, R. A., Govind Kumar, Shailendra Kumar Maurya, S. Rajan, Israr Ahmad, and A. Kumar. "Plant growth promoting, biochemical and antifungal properties of microbes isolated from cow dung." JOURNAL OF ECO-FRIENDLY AGRICULTURE 18, no. 2 (2023): 248–53. http://dx.doi.org/10.48165/jefa.2023.18.02.4.

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Cow dung is a rich source of microbial diversity and being used since long time in India for seed treatment, plastering cut ends of vegetative propagated plant parts and sprinkling of diluted solution on crops. In present study, microbes from fresh cow dung of indigenous cow were isolated and biochemically characterized. Cow dung enriched with diversity of microorganisms in different medium i.e 38 x 106cfu g-1 (Nutrient agar), 26.0 x 106 cfu g-1 (Actinomycetes isolation agar), 36 x 106 cfu g-1 (Kings B agar), 16 x 106 cfu g-1(CV agar),13 x 106 cfu g-1 (Methyl red), 15 x 106 cfu g-1 (Pikovskyas

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Zahoor, Sarmad, Naveed Shehzad Ahmad, Sadia Asif, et al. "Antibiotic susceptibility of bacterial strains isolated from urinary tract infections inPakistan." International Journal of Medicine 9, no. 1 (2021): 18. http://dx.doi.org/10.14419/ijm.v9i1.31359.

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Background: Urinary track infection (UTI) is a common infection in Pakistan. Poor knowledge about personal hygiene and lack of awareness are major reasons of this infection. All over the world and in Pakistan E. coli and K. pneumonia are main causative agents of UTI.Objectives:To analyze Antibiotic susceptibility pattern of bacterial strains isolated from urinary tract infections.Methods: In present study total 227 samples from Genomic Research Lab and Diagnostic Center Rawalpindi, Pakistan were tested for presence of different microbial infectious agents and their antibiotic resistance and su

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Abduljalil, Nafisa Adamu, Oche Clement Abah, and Hafsat Bakori Sagir. "Incidence of Typhoid Fever Infection among Febrile Patients attending FUDMA Clinic." Sahel Journal of Life Sciences FUDMA 2, no. 3 (2024): 1–5. http://dx.doi.org/10.33003/sajols-2024-0203-01.

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Typhoid fever remains a public health concern in Nigeria, with its incidence poising substantial challenges to health care systems. Widal agglutination test is largely the only used diagnostic test method for the laboratory diagnosis of Typhoid infection which has been proven to be neither sensitive nor specific. This paper delves into the incidence, contributory factors as well as sensitivity and specificity of commonly used diagnostic tests of typhoid fever infection among febrile patients attending the FUDMA Clinic. A total of 55 Blood and stool samples were analyzed using standard procedur

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Pandey, S., and A. Alam. "Isolation of endophytic bacteria from bryophytes and study of their morphological, biochemical and biofilm formation properties." Journal of Environmental Biology 44, no. 3 (2023): 351–58. http://dx.doi.org/10.22438/jeb/44/3/mrn-5079.

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Aim: To isolate an endophytic bacterial community associated with bryophytes, which were collected from various locations in Mount Abu, Rajasthan, and to characterise their morphological, biochemical, and biofilm development properties. Methodology: Different bryophytes were selected from various regions of Mount Abu, Rajasthan. On an R2A agar plate, colonies with various morphologies were isolated. Biofilm development of bacterial isolates was done on a glass surface and visualised through scaning electron microscopy. Biochemical test, such as indole test, catalase test, urease test, MR and V

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Çağlayan, Pınar. "Characterization of Planococcus dechangensis isolated from a water sample of Çamaltı Saltern." Ege Journal of Fisheries and Aquatic Sciences 38, no. 4 (2021): 527–31. http://dx.doi.org/10.12714/egejfas.38.4.15.

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In the present study, strain MHDS3 was isolated from a water sample of Çamaltı Saltern and identified using conventional and molecular methods. 16S rRNA gene sequence analyses showed that the strain MHDS3 belonged to Planococcus dechangensis species. It gave a positive result in the Gram staining test. The cells were coccus, non-motile, aerobic, catalase positive, oxidase negative and the colony pigmentation was yellow-orange. It showed negative results for citrate utilization, indole production from tryptophane, Voges-Proskauer and methyl red. This isolate was able to grow at 10-45°C (optimal

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Peltroche-Llacsahuanga, Heidrun, Norbert Schnitzler, Rudolf Lütticken, and Gerhard Haase. "Rapid Identification of Candida glabrataby Using a Dipstick To Detect Trehalase-Generated Glucose." Journal of Clinical Microbiology 37, no. 1 (1999): 202–5. http://dx.doi.org/10.1128/jcm.37.1.202-205.1999.

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Candida glabrata is a yeast frequently isolated from human specimens. Based upon its well-known ability to rapidly hydrolyze trehalose, we have developed a novel and cost-effective test incubating one yeast colony emulsified in 50 μl of citrate buffer (0.1 M [pH 5.0]) containing 4% (wt/vol) trehalose for 3 h at 37°C. Trehalase-generated glucose is detected with a commercially available dipstick (range, 1.0 to 50 g/liter). For evaluation, consecutive clinical isolates and several reference strains of C. glabrata (n = 160), C. albicans(n = 120), and other yeast species with potential ability for

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Abdul, Qadir Zafar Ahmad Kamran Taj Abdul Samad Muhammad Rizwan Muhammad Ali Muhammad shafee Shehzad Ahmad Mujahid Farooq. "BIOMOLECULAR IDENTIFICATION OF STAPHYLOCOCCUS AUREUS AND SCREENING OF METHICILLIN RESISTANT STRAIN FROM PUS /WOUND SAMPLE IN TERTIARY CARE HOSPITAL QUETTA." INDO AMERICAN JOURNAL OF PHARMACEUTICAL SCIENCES 05, no. 08 (2018): 7532–39. https://doi.org/10.5281/zenodo.1345127.

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The aim of this study was the bio-molecular identification of Staphylococcus aureus and screening of methicillin resistant strain. To evaluate the study, 60 samples of pus and wound were collected from Bolan Medical Complex Hospital Quetta, Balochistan during period from September 2016 to December 2017. Out of 60 samples processed for isolation, ten (10) were found positive for staphylococcus aureus. Isolates were recognized by growth characteristic on selective media, Gram staining and different biochemical tests like oxidase, catalase, coagulase, motility, thermonuclease (DNase), haemolysis

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Begum, Amina, Asma Jabeen, Rizwana Lakho, Zakia Zaheen, Madhu Bala, and Arooba Bhutto. "Prevalence of Urinary Tract Infection during Pregnancy at Tertiary Care Hospital: A Cross-Sectional Study." Pakistan Journal of Medical and Health Sciences 16, no. 5 (2022): 851–53. http://dx.doi.org/10.53350/pjmhs22165851.

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Aim: the aim of this study was to determine the prevalence of urinary tract infection during pregnancy and the microorganism associated with it. Study design: A cross-sectional study Place and Duration: This study was conducted at CDF Hospital Hyderabad, Pakistan from June 2020 to June 2021 Methodology: A total of 150 women were included in this study. All the women were pregnant and aged between 18 to 50 years. Urine samples were collected from all the participants. From each sample, bacteria were isolated, and then the isolated bacteria were stained. Also, indole test, catalase test, voges p

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Patwekar, Mohsina, Faheem Patwekar, Saad Alghamdi, et al. "Vancomycin as an Antibacterial Agent Capped with Silver Nanoparticles: An Experimental Potential Analysis." BioMed Research International 2022 (August 21, 2022): 1–8. http://dx.doi.org/10.1155/2022/3682757.

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For the treatment of various infections, a variety of antimicrobial drugs are formulated. Nevertheless, many bacterial infections now exhibit antibiotic resistance due to the widespread utilization antibiotics. Methicillin-resistant among the most dangerous multidrug-resistant bacteria is Staphylococcus aureus (MRSA). Vancomycin became a viable therapy option due to MRSA resistance to methicillin medicines. One of the well-informed antibacterial compounds with wideband antibacterial activity is silver nanoparticles (AgNPs). AgNPs are thus suitable candidates for usage in conjunction alongside

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Samer M. Al-Hulu. "Antibacterial activity of Metal nanoparticles produced by Streptomyces spp. Isolated from soil samples." International Journal of Research in Pharmaceutical Sciences 11, no. 4 (2020): 5042–46. http://dx.doi.org/10.26452/ijrps.v11i4.3097.

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Twenty-five soil samples were collected from Hilla city. (10) Actinomyctes isolates were isolated. Five Streptomyces spp. Late was diagnosed. Isolates positive for a gram and having grey Aerial Mycelium and yellow-green Substrate mycelium on yeast-malt extract medium. All Streptomyces spp. olates d for nanoparticles production. Streptomyces spp.2 was able for producing of omyces spp.2 was, grey aerial, yellow-green substrate mycelium, unable for in producing, having the ability for using glucose, sucrose, mannitol, negative for fructose and mannose, negative for indole, and vogas Proskauer, po

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Rzigalinski, Beverly A., Holly M. Giovinco, and Byron J. Cheatham. "Cerium Oxide Nanoparticles Improve Lifespan of Stored Blood." Military Medicine 185, Supplement_1 (2020): 103–9. http://dx.doi.org/10.1093/milmed/usz210.

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ABSTRACT Introduction Blood is a precious commodity, with storage limited to 42 days under refrigeration. Degradative changes in red blood cells (RBCs) begin as early as 11–21 days after collection, and compromise their function. Materials that extend the life of RBCs will improve blood utilization in the field, as well as in hospital settings. Cerium oxide nanoparticles (CeONPs) are widely used in the materials industry to counteract oxidative stress and improve oxygen storage. We have previously shown that CeONPs extended the lifespan of cells in culture and counteract oxidative stress in vi

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Kasem Hussein, Humam. "Isolation and Detection of Moraxella catarrhalis from children Infected with Acute Otitis Media in Al-Kadhemiya Pediatric Hospital." Al-Kufa University Journal for Biology 7, no. 1 (2015): 28–39. http://dx.doi.org/10.36320/ajb/v7.i1.8099.

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A study was carried out to assess isolation and detection of Moraxella catarrhalis(M. catarrhalis) isolates from 100 specimens received from Children at 1-3 years of age suffered with Acute Otitis Media (AOM) in Al-Kadhemiya Pediatric Hospital.Methods: The identity of isolates was confirmed by staining method (Gram stain) , culturing, biochemical test(catalase, citrate utilization, indole production, urease production, motility, methyl red, Voges-Proskauer and DNase production) and Sensitivity test.Results: from 100 samples, 8 isolates of M. catarrhalis were collected. The biochemical activiti

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Joshi, Bhavesh M., Mrugesh M. Patel, Rudra B. Parmar, N. K. Singh, and P. V. Tapre. "Molecular Characterization of Plant Growth Promoting Pseudomonas from Rhizosphere of Euphorbia caducifolia Haines." International Journal of Bio-resource and Stress Management 13, no. 11 (2022): 1240–51. http://dx.doi.org/10.23910/1.2022.3262.

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A study was conducted during kharif 2020 at the Department of Microbiology, Chimanbhai Patel College of Agriculture, S. D. Agricultural University for molecular characterization of plant growth promoting Pseudomonas isolates from rhizosphere of Euphorbia caducifolia. Twenty Pseudomonas isolates (designated as PsEc1 to PsEc20) were axenically isolated from rhizospheric soil sample of Euphorbia caducifolia using Pseudomonas Isolation Agar Base (a selective medium). These isolates were tested gram negative, rod shaped, and oxidase positive. All 20 isolates were positive for utilization of maltose

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