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1

Bentahar, Assia, Amel Bouaziz, Saliha Djidel, and Seddik Khennouf. "Phenolic content and antioxidant activity of ethanolic extracts from Citrus sinensis L. and Citrus reticulata L. fruits." Journal of Drug Delivery and Therapeutics 10, no. 5-s (October 30, 2020): 308–13. http://dx.doi.org/10.22270/jddt.v10i5-s.4537.

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Citrus fruits, in particular the genus Citrus, are very rich in antioxidants which have beneficial effects on human health. The objective of this study was to evaluate the phenolic content and the antioxidant activity of the ethanolic extracts from orange (Citrus sinensis L.) and mandarin (Citrus reticulata L.) fruits. The quantitative estimation of the total phenolic content in the two extracts revealed their richness in these compounds. The results obtained showed that the polyphenols content were 159.66 ± 2.62 mg GAE / g of dry extract and 127.33 ± 2.32 mg GAE / g of dry extract for C. sinensis and C. reticulata, respectively.The quantification of flavonoids content showed that the ethanolic extracts of C. sinensis and C. reticulata contained 0.85 ± 0.01 mg QE / g of dry extract and 0.876 ± 0.073 mg QE / g of dry extract, respectively. Whereas, the tannins contents were 46.32 ± 1.02 mg TAE / g of dry extract for C. sinensis and 47.65 ± 1.36 mg TAE / g of dry extract for C. reticulata. In vitro antioxidant activity was evaluated using two tests: the reducing power and the hydroxyl radical scavenger test. The evaluation of the two extracts by the ferric iron reducing power test showed an EC50 value of 0.882 ± 0.037 mg / ml for C. sinensis extract and an EC50 of 1.085 ± 0.068 mg / ml for C. reticulata extract. In addition, C. sinensis and C. reticulata showed good hydroxyl radical scavenging effect with IC50 values of 0.303 ± 0.026 mg /ml and 0.572 ± 0.100 mg / ml for C. sinensis and C. reticulata, respectively. These results suggested that these fruit extracts could be good sources of phenolic compounds and ingredients with high antioxidant potential. Keywords: Citrus sinensis, Citrus reticulata, Polyphenols, Flavonoids, Tannins, Antioxidant activity.
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2

Haag, H. P., L. E. Gutierrez, A. R. Dechen, F. A. A. Mourão Filho, and C. S. Moreira. "Variação de matéria seca e de nutrientes nas folhas e nos frutos, produção de ácido ascórbico e suco, em seis cultivares de citros, durante um ciclo." Scientia Agricola 50, no. 2 (September 1993): 193–203. http://dx.doi.org/10.1590/s0103-90161993000200005.

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De uma plantação de citros, com os cultivares T. Cravo (Citrus reticulata Blanco), L.Hamlin (Citrus sinensis (L.) Osbeck), T. Murcott (Citrus reticulata Blanco x Citrus sinensis (L.) Osbeck), L. Natal (Citrus sinensis (L.) Osbeck, L. Valencia (Citrus sinensis (L.) Osbeck) e L. Pera (Citrus sinensis (L.) Osbeck), situada na "Fazenda Sete Lagoas", no município de Mogi-Guaçu (22° 22% 46° 56'W.Gr.), em Latossolo Vermelho amarelo, fase arenosa, foram coletados frutos 30 dias após florescimento, até a idade da coleta comercial. No material coletado, foram determinadas a variação da matéria seca, a concentração dos macro e micronutrientes nas folhas adjacentes ao fruto, a extração de macro e micronutríentes pelos frutos, a produção de suco (ml) por fruto e a concentração de ácido ascórbico (mg/100 ml de suco). Concluiu-se que: 1. O aumento da matéria seca, intensifica-se a partir do segundo mês apos o florescimento; 2. Com exceção da T. Cravo, ocorre uma diminuição na produção de matéria seca no final do ciclo; 3. A concentração dos macro e micronutrientes nas folhas apresenta oscilações durante o desenvolvimento do fruto; 4. A ordem decrescente de extração de nutrientes é: K, N, Ca, Mg, P = S, Fe, B, Zn, Mn, Cu; 5. A capacidade de exportação de nutrientes pelos cultivares é, em ordem decrescente: L. Pera, L. Hamlin = T. Cravo, T. Murcott, L. Valencia, L. Natal; 6. A quantidade de suco produzido por fruto, oscila entre 43 a 95 ml; 7. A concentração de ácido ascórbico (mg/100 ml de suco), varia entre 30 a 95.
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3

VIUDA-MARTOS, M., Y. RUIZ-NAVAJAS, J. FERNÁNDEZ-LÓPEZ, and J. PEREZ-ÁLVAREZ. "ANTIBACTERIAL ACTIVITY OF LEMON (CITRUS LEMON L.), MANDARIN (CITRUS RETICULATA L.), GRAPEFRUIT (CITRUS PARADISI L.) AND ORANGE (CITRUS SINENSIS L.) ESSENTIAL OILS." Journal of Food Safety 28, no. 4 (October 22, 2008): 567–76. http://dx.doi.org/10.1111/j.1745-4565.2008.00131.x.

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4

Viuda-Martos, M., Y. Ruiz-Navajas, J. Fernández-López, and J. Pérez-Álvarez. "Antifungal activity of lemon (Citrus lemon L.), mandarin (Citrus reticulata L.), grapefruit (Citrus paradisi L.) and orange (Citrus sinensis L.) essential oils." Food Control 19, no. 12 (December 2008): 1130–38. http://dx.doi.org/10.1016/j.foodcont.2007.12.003.

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5

Fleisher, Zhenia, and Alexander Fleisher. "Sweet Orange Leaf Oil (Citrus sinensis(L.) Osbeck)." Journal of Essential Oil Research 2, no. 4 (July 1990): 203–5. http://dx.doi.org/10.1080/10412905.1990.9697862.

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6

Liu, Xing Yan, Meng Lin Fan, Huai You Wang, Bo yang Yu, and Ji Hua Liu. "Metabolic profile and underlying improved bio-activity of Fructus aurantii immaturus by human intestinal bacteria." Food & Function 8, no. 6 (2017): 2193–201. http://dx.doi.org/10.1039/c6fo01851c.

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7

Fahrurroji, Andhi, and Hafrizal Riza. "Karakterisasi Ekstrak Etanol Buah Citrus amblycarpa (L), Citrus aurantifolia (S.), dan Citrus sinensis (O.)." JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA 7, no. 2 (November 30, 2020): 100. http://dx.doi.org/10.20473/jfiki.v7i22020.100-113.

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Pendahuluan: Buah jeruk manis (Citrus amblycarpa (L)), jeruk nipis (Citrus aurantifolia (S.)), dan jeruk sambal (Citrus sinensis (O.)) memiliki potensi yang sangat besar khususnya kandungan senyawa metabolit sekunder. Tujuan: Pada penelitian ini bertujuan untuk mengetahui karakteristik spesifik dan non spesifik berbagai jenis buah jeruk tersebut pada pelarut etanol 60%. Metode: Ekstrak yang diperoleh ditentukan parameter nonspesifik dan parameter spesifik ekstraknya dari masing-masing ekstrak dari komponen buah jeruk tersebut. Hasil: Berdasarkan hasil pengamatan yang dilakukan Ekstrak etanol 60% kulit dan biji serta air pada buah jeruk tersebut dengan nilai rendemen tertinggi diperoleh pada ekstrak etanol 60% biji jeruk sambal dengan rendemen yang dihasilkan sebesar 25,28%. Seluruh sampel mengandung senyawa fenolik dan flavonoid pada uji tabung. Pada uji KLT fase diam silica gel 60 F254 untuk identifikasi saponin dengan fase gerak kloroform : metanol : air (64 : 50 : 1) dan identifikasi flavonoid menggunakan fase gerak asam asetat glasial : asam formiat : air : etil asetat (11 : 11 : 27 : 100) pada kedua senyawa tersebut menunjukkan reaksi positif pada seluruh sampel uji. Kadar air untuk sampel air jeruk berkisar antara 8,149% - 9,748%. Kesimpulan: Nilai rendemen tertinggi diperoleh pada ekstrak etanol 60% biji jeruk sambal sebesar 25,28%. Pada uji KLT fase diam silica gel 60 F 254 untuk identifikasi saponin dan flavonoid seluruh sampel menunjukkan reaksi positif. Serta kadar abu seluruh sampel pada rentang 2,722 - 5,442%.
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Družić, Jasmina, Igor Jerković, Zvonimir Marijanović, and Marin Roje. "Chemical biodiversity of the leaf and flower essential oils of Citrus aurantium L. from Dubrovnik area (Croatia) in comparison with Citrus sinensis L. Osbeck cv. Washington navel, Citrus sinensis L. Osbeck cv. Tarocco and Citrus sinensis L. Osbeck cv. Doppio Sanguigno." Journal of Essential Oil Research 28, no. 4 (March 11, 2016): 283–91. http://dx.doi.org/10.1080/10412905.2016.1159258.

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9

Almeida, Weliton Antonio Bastos de, Francisco de Assis Alves Mourão Filho, Beatriz Madalena Januzzi Mendes, Alexandra Pavan, and Adriana Pinheiro Martinelli Rodriguez. "Agrobacterium-mediated transformation of Citrus sinensis and Citrus limonia epicotyl segments." Scientia Agricola 60, no. 1 (February 2003): 23–29. http://dx.doi.org/10.1590/s0103-90162003000100005.

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Genetic transformation allows the release of improved cultivars with desirable characteristics in a shorter period of time and therefore may be useful in citrus breeding programs. The objective of this research was to establish a protocol for genetic transformation of Valencia and Natal sweet oranges (Citrus sinensis L. Osbeck) and Rangpur lime (Citrus limonia L. Osbeck). Epicotyl segments of germinated in vitro plantlets (three weeks in darkness and two weeks in a 16-h photoperiod) were used as explants. These were co-cultivated with Agrobacterium tumefaciens strain EHA-105 and different experiments were done to evaluate the transformation efficiency: explants were co-cultivated with Agrobacterium for one, three or five days; explants were incubated with Agrobacterium suspension for 5, 10, 20 or 40 minutes; co-cultivation medium was supplemented with acetosyringone at 0, 100 or 200 µmol L-1; Explants ends had a longitudinal terminal incision (2-3 mm); co-cultivation temperatures of 19, 23 or 27°C were imposed. The experimental design was completely randomized in all experiments with five replications, each consisted of a Petri dish (100 x 15 mm) with 30 explants and resulted in a total of 150 explants per treatment. Longitudinal terminal incision in the explant ends did not improve shoot regeneration. However, transgenic plants of all three cultivars were confirmed from explants that had been subjected to inoculation time of 20 minutes, co-culture of three days at 23-27°C, in the absence of acetosyringone.
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10

Zhou, Carol L. Ecale, El-Desouky Ammar, Hany Sheta, Sandra Kelley, MaryLou Polek, and Diane E. Ullman. "Citrus tristeza virus ultrastructure and associated cytopathology in Citrus sinensis and Citrus aurantifolia." Canadian Journal of Botany 80, no. 5 (May 1, 2002): 512–25. http://dx.doi.org/10.1139/b02-030.

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Citrus tristeza virus ultrastructure and associated cytopathology was documented with three isolates and two hosts, sweet orange 'Madam vinous' (Citrus sinensis (L.) Osbeck) and Mexican lime (Citrus aurantifolia (L.) Swingle). Virions were long, flexuous, and disorganized or in swirled, parallel masses. Infection was common in phloem parenchyma and companion cells and less frequent in mature sieve elements. Immunogold labeling confirmed previous findings that the major coat protein encapsidated the length of purified virions, while the minor coat protein encapsidated one terminal. Three types of inclusions were observed: (i) viral arrays that reacted with antibodies against the major (p25) and minor (p27) Citrus tristeza virus coat proteins, (ii) fibrous inclusions that reacted with antibodies against the Citrus tristeza virus p20 gene product but were sparsely labeled with antibodies against either coat protein, and (iii) accumulated cytoplasmic vesicles associated with aggregated, vesiculating mitochondria. The latter resembled Beet yellows virus-like vesicles, which are typical of closterovirus infection, but did not react with any of our antibodies. Cytopathology did not differ between isolates and plant hosts. Most effects were observed in phloem parenchyma cells, including chloroplast degradation, mitochondria vesiculation, and nuclear membrane invagination. Multivesicular bodies and lipid-filled vesicles were abundant in the cytoplasm. Masses of electron-lucent vesicles and electron-dense bodies were present between the cell membrane and cell wall.Key words: immunolocalization, CTV major coat protein, CTV minor coat protein, CTV p20 gene product, inclusions, isolate severity.
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11

Mendes-da-Glória, Fernanda Januzzi, Francisco de Assis Alves Mourão Filho, Clarice Garcia Borges Demétrio, and Beatriz Madalena Januzzi Mendes. "Embryogenic calli induction from nucellar tissue of Citrus cultivars." Scientia Agricola 56, no. 4 suppl (1999): 111–1115. http://dx.doi.org/10.1590/s0103-90161999000500012.

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Nucellar tissues of seven Citrus varieties were introduced onto three growth media to produce embryogenic callus. The media tested were: EME [MT, modified, with the addition of malt extract (500 mg.L-1)]; 1/2-EME [half concentration of MT macronutrients + half concentration of BH3 macronutrients + 500 mg.L-1 malt extract + 1.55 g.L-1 of glutamine]; and EBA [EME + 0.44 muM 6-benzyladenine + 0.04 muM 2,4 D]. Soft friable calli were obtained from 'Cravo' and 'Ponkan' mandarins (Citrus reticulata, Blanco), 'Murcott' tangor (Citrus reticulata Blanco x Citrus sinensis L. Osbeck), 'Serra d'água' and 'Valencia' sweet oranges (Citrus sinensis, L. Osbeck) 120 days after callus induction. 'Natal' and 'Pera' sweet oranges (Citrus sinensis, L. Osbeck) produced hard non-friable calli in this period. EME and 1/2-EME media had the best results for 'Cravo' mandarin, 'Ponkan' mandarin and 'Serra d'água' sweet orange, whereas EBA was the best media composition to induce soft friable calli on 'Murcott' tangor and 'Valencia' sweet orange. Friable callus cultures of 'Cravo' and 'Ponkan' mandarins, and 'Murcott' tangor yielded high quality protoplasts after isolation. Abbreviations: a.c. - activated charcoal; BA - 6-benzyladenine; IAA - indole-acetic acid; 2,4-D - 2,4-diclorophenoxyacetic acid; MT - Murashige & Tucker basal medium.
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12

Girardi, Eduardo Augusto, and Francisco de Assis Alves Mourão Filho. "Production of interstocked 'Pera' sweet orange nursey trees on 'Volkamer' lemon and 'Swingle' citrumelo rootstocks." Scientia Agricola 63, no. 1 (February 2006): 5–10. http://dx.doi.org/10.1590/s0103-90162006000100002.

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Incompatibility among certain citrus scion and rootstock cultivars can be avoided through interstocking. 'Pera' sweet orange (Citrus sinensis L. Osbeck) nursery tree production was evaluated on 'Swingle' citrumelo (Poncirus trifoliata (L.) Raf x Citrus paradisi Macf) and 'Volkamer' lemon (Citrus volkameriana Pasquale) incompatible rootstocks, using 'Valencia' and 'Hamlin' sweet oranges (Citrus sinensis L. Osbeck), 'Sunki' mandarin (Citrus sunki Hort. ex Tanaka), and 'Cleopatra' mandarin (Citrus reshni Hort. ex Tanaka) as interstocks. Citrus nursery trees interstocked with 'Pera' sweet orange on both rootstocks were used as control. 'Swingle' citrumelo led to the highest interstock bud take percentage, the greatest interstock height and rootstock diameter, as well as the highest scion and root system dry weight. Percentage of 'Pera' sweet orange dormant bud eye was greater for plants budded on 'Sunki' mandarin than those budded on 'Valencia' sweet orange. No symptoms of incompatibility were observed among any combinations of rootstocks, interstocks and scion. Production cycle can take up to 17 months with higher plant discard.
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13

FIGUEIREDO, JOSÉ ORLANDO DE, EDUARDO SANCHES STUCHI, LUIZ CARLOS DONADIO, JOAQUIM TEÓFILO SOBRINHO, FRANCISCO FERRAZ LARANJEIRA, ROSE MARY PIO, and OTÁVIO RICARDO SEMPIONATO. "Porta-enxertos para a lima-ácida-'Tahiti' na região de Bebedouro, SP." Revista Brasileira de Fruticultura 24, no. 1 (April 2002): 155–59. http://dx.doi.org/10.1590/s0100-29452002000100034.

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Foi instalado um experimento de seleção de porta-enxertos para a lima-ácida-'Tahiti' (Citrus latifolia Tanaka), em dezembro de 1988, na Estação Experimental de Citricultura de Bebedouro-SP, com o objetivo de conhecer seu comportamento e oferecer novas opções de plantio para as condições ecológicas semelhantes às daquela região. A variedade copa, originária do BAG-Citros do IAC, localizado no Centro de Citricultura Sylvio Moreira, Cordeirópolis-SP, é um clone nucelar de 'Tahiti', denominado IAC-5. Os porta-enxertos, que tiveram a mesma origem, foram: tangerinas-'Sunki' (Citrus sunki Hort. ex Tanaka); 'Cleópatra'(Citrus reshni Hort. ex Tan.); 'Batangas' e 'Oneco' (Citrus reticulata Blanco); trifoliata-EEL (Poncirus trifoliata Raf.); limão-'Cravo' (Citrus limonia Osbeck); limão-'Volkameriano Catania 2' (Citrus volkameriana Tan. & Pasq.); tangelo-'Orlando' (C. reticulata Blanco x Citrus paradisi Macf.); citrumelo-'Swingle' (P. trifoliata Raf. X C.paradisi Macf.); citrange-'Morton' (P. trifoliata Raf. X C. sinensis (L.) Osbeck) e laranja-'Caipira DAC' (C. sinensis (L.) Osbeck). Com relação à produção, avaliada no período de 1991 a 1998, os porta-enxertos de melhor comportamento foram o tangelo-'Orlando', citrange-'Morton' e citrumelo-'Swingle'. As mais baixas produções ocorreram nos porta-enxertos de tangerina e de laranja-'Caipira DAC'. O limão-'Cravo' apresentou produção intermediária e proporcionou curta vida útil às plantas.
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Kasali, Adeleke A., Oladipupo A. Lawal, Adeolu O. Eshilokun, Abayomi A. Olaniyan, Andy R. Opoku, and William N. Setzer. "Citrus Essential Oil of Nigeria Part V: Volatile Constituents of Sweet Orange Leaf Oil (Citrus sinensis)." Natural Product Communications 6, no. 6 (June 2011): 1934578X1100600. http://dx.doi.org/10.1177/1934578x1100600629.

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The volatile oils extracted from leaves of eight cultivars of Citrus sinensis (L) Osbeck were comprehensively analysed by a combination of GC and GC-MS. Fifty four constituents accounting for 82.3-98.2% were identified. Sabinene (20.9-49.1%), δ-3-carene (0.3-14.3%), ( E)-β-ocimene (4.4-12.6%), linalool (3.7-11.1%) and terpinen-4-ol (1.7-12.5%) were the major constituents that are common to all the volatile oils. In addition, a cluster analysis was carried out and indicated at least four different chemotypes for the C. sinensis cultivars.
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15

Hagenmaier, Robert D., and Robert A. Baker. "Cleaning Method Affects Shrinkage Rate of Citrus Fruit." HortScience 28, no. 8 (August 1993): 824–25. http://dx.doi.org/10.21273/hortsci.28.8.824.

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The shrinkage rate of `Marsh' grapefruit (Citrus paradisi Macf.), `Ambersweet' hybrid [(C. reticulata Blanco × C. paradisi Macf. × C. reticulata) × C. sinensis (L.) Osb.] and `Valencia' oranges [C. sinensis (L.) Osb.] was increased 50 % to 150% by washing the fruit with rotary brushes, but was not changed by hand-washing the fruit with cellulose sponges. Internal CO2 increased using both washing methods. Waxed fruit obtained from five Florida packinghouses and cleaned with rotary brushes and waxed had shrinkage rates the same as those of nonwashed controls. Thus, controlling the washing process is important to minimize shrinkage of fresh citrus fruit.
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Calixto, Marcia Cristina, Francisco de Assis Alves Mourão Filho, Beatriz Madalena Januzzi Mendes, and Maria Lúcia Carneiro Vieira. "Somatic hybridization between Citrus sinensis (L.) Osbeck and C. grandis (L.) Osbeck." Pesquisa Agropecuária Brasileira 39, no. 7 (July 2004): 721–24. http://dx.doi.org/10.1590/s0100-204x2004000700015.

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This work had as objective to produce citrus somatic hybrids between sweet oranges and pummelos. After chemical fusion of sweet orange embryogenic protoplasts with pummelo mesophyll-derived protoplasts, plants were regenerated by somatic embryogenesis and acclimatized in a greenhouse. The hybrids of 'Hamlin' sweet orange + 'Indian Red' pummelo and 'Hamlin' sweet orange + 'Singapura' pummelo were confirmed by leaf morphology, chromosome counting and molecular analysis. These hybrids have potential to be used directly as rootstocks aiming blight, citrus tristeza virus, and Phytophthora-induced disease tolerance, as well as for rootstocks improvement programs.
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Abbate, Loredana, Nicasio Tusa, Sergio Fatta Del Bosco, Tonia Strano, Agatino Renda, and Giuseppe Ruberto. "Genetic improvement of Citrus fruits: New somatic hybrids from Citrus sinensis (L.) Osb. and Citrus limon (L.) Burm. F." Food Research International 48, no. 1 (August 2012): 284–90. http://dx.doi.org/10.1016/j.foodres.2012.04.007.

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18

Reddy, Krishna N., and Megh Singh. "Response of Citrus (Citrus spp.) Rootstock Seedlings to Soil-Applied Herbicides." Journal of Environmental Horticulture 11, no. 1 (March 1, 1993): 39–40. http://dx.doi.org/10.24266/0738-2898-11.1.39.

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Abstract A greenhouse study was conducted to evaluate the response of Carrizo citrange [Citrus sinensis (L.) Obs. × Poncirus trifoliata (L.) Raf.], Cleopatra mandarin (C. reticulata Blanco), sour orange (C. aurantium L.), and Swingle citrumelo [C. paradisi Macf. × P. trifoliata (L.) Raf.] citrus rootstock seedlings to multiple applications of Premier (fluometralin), Dual (metolachlor), Devrinol (napropamide), Solicam (norflurazon), Surflan (oryzalin), Prowl (pendimethalin), and Treflan trifluralin). The citrus rootstock responses ranged from a 11% reduction in shoot weight of sour orange with Treflan (trifluralin) to a 19% reduction in fibrous root weight of Swingle citrumelo with Surflan (oryzalin). Cleopatra mandarin was tolerant to all the seven herbicides. Dual (metolachlor), Devrinol (napropamide), Solicam (norflurazon), and Premier (fluometralin) were not phytotoxic to all four rootstocks.
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Santos, Carlos Henrique dos, Jaime Duarte Filho, Junior Cesar Modesto, Hélio Grassi Filho, and Gisela Ferreira. "Adubos foliares quelatizados e sais na absorção de boro, manganês e zinco em laranjeira ‘Pera’." Scientia Agricola 56, no. 4 (October 1999): 999–1004. http://dx.doi.org/10.1590/s0103-90161999000400031.

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O presente trabalho teve como objetivo comparar a eficiência de formulações de adubos foliares quelatizados na absorção dos micronutrientes boro, manganês e zinco, com a aplicação convencional de sais em plantas de laranjeira ‘Pera’ (Citrus sinensis (L.) Osbeck). Para tanto foi conduzido experimento nas dependências do Departamento de Ciência do Solo da Faculdade de Ciências Agronômicas UNESP/Campus de Botucatu, Estado de São Paulo. Utilizaram-se plantas de laranjeira ‘Pera’ (Citrus sinensis (L.) Osbeck) enxertadas sobre limoeiro ‘Cravo’ (Citrus limonia Osbeck), com 2 anos de idade, plantadas em caixas de 250 litros. Os adubos foliares utilizados foram: Grex Citros na dose de 1,0 mL L-1; Copas citros 2,0 mL L-1; Plantin Citros 1,0 mL L-1; Citrolino 2,0 mL L-1; Fertamin Citros 1,75 mL L-1; Yogen Citros 2,0 mL L-1; MS-2 1,0 mL L-1; Sais, Sais + 1,0 g L-1 de KCl e Sais substituindo o ZnSO4 pelo ZnCl2. O volume de aplicação, foi de 1 litro de calda planta-1. Em todos os tratamentos adicionou-se o espalhante adesivo do grupo químico dos alquifenoletoxilados a 0,03%. A amostragem das folhas foi realizada 30 dias após a aplicação dos tratamentos, coletando-se a 3a ou 4a folha de ramos vegetativos no início do florescimento, dos 4 quadrantes, localizados na região mediana da planta, totalizando 10 folhas por planta. A aplicação foliar de micronutrientes, favoreceu a absorção e resultou no aumento do teor foliar de Mn e Zn mas não de B, sendo que a presença de cloreto aumentou os teores de Zn na folhas de laranjeira ‘Pera’, proporcionando maior absorção do que o sulfato e sulfato adicionado ao cloreto de potássio. Os resultados mostram, também, que os produtos quelatizados Yogen e MS-2, para as condições deste estudo, não foram eficientes como fontes fornecedoras de Mn.
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Kayum, Mukadder, N. Kemal Koç, and Veli-Matti Rokka. "Variation of the Nuclear DNA Content of Species of Subtribe Citrinae (Rutaceae)." HortScience 33, no. 7 (December 1998): 1247–50. http://dx.doi.org/10.21273/hortsci.33.7.1247.

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Laser flow cytometry was used to analyze nuclear DNA contents (2C values) of five genera (Severinia Ten., Atalantia Corrèa, Fortunella Swing., Poncirus Raf., and Citrus L.) taxonomically grouped in subtribe Citrinae (citrus fruit trees) of the Rutaceae. The genotypes analyzed had 2C values ranging from 0.67 pg for diploid Severinia buxifolia (Poir.) Ten. to 1.27 pg for tetraploid Hongkong Fortunella hindsii Swing. There was no significant difference in the 2C values within the sexually compatible diploid species of 11 “true citrus fruit trees” [Citrus aurantium L., C. grandis (L.) Osbeck, C. limon (L.) Burm. f., C. limonia Osbeck, C. paradisi Macf., C. reshni Hort. ex Tanaka, C. sinensis (L.) Osbeck, C. volkameriana Ten. & Pasq., Poncirus trifoliata (L.) Raf., and the intergeneric hybrid C. sinensis × P. trifoliata]. The species Atalantia ceylanica (Arn.) Oliv. (a “near-citrus fruit tree”), sexually incompatible with Citrus spp., had a 2C value significantly different from those of the true citrus fruit tree species. The 2C value of Severinia buxifolia (a “primitive citrus fruit tree”), another species sexually incompatible with the Citrus spp., also differed from those of some of the true citrus fruit tree species. The data largely corresponds with taxonomical differences between a) the genera Citrus and Poncirus and b) the genera Severinia and Atalantia, all assigned to subtribe Citrinae.
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Toledo, V. A. A., M. C. C. R. Takasusuki, T. V. Baitala, F. M. C. Maia, H. L. Pereira, A. L. Halak, E. D. Chambó, and D. T. M. Souza. "Polinização por Abelhas (Apis mellifera L.) em Laranjeira (Citrus sinensis L. Osbeck)." Scientia Agraria Paranaensis 12, no. 4 (December 30, 2013): 236–46. http://dx.doi.org/10.18188/1983-1471/sap.v12n4p236-246.

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22

Khelker, Tuneer, Nurul Haque, and Arvind Agrawal. "Ultraviolet Protection potential of Curcuma longa L. and Citrus sinensis (L.) Osbeck." Research Journal of Pharmacy and Technology 10, no. 12 (2017): 4282. http://dx.doi.org/10.5958/0974-360x.2017.00784.3.

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23

Grosser, Jude W., Frederick G. Gmitter, J. L. Chandler, and Eliezer S. Louzada. "Somatic Hybridization of Complementary Citrus Rootstock:Five New Hybrids." HortScience 29, no. 7 (July 1994): 812–13. http://dx.doi.org/10.21273/hortsci.29.7.812.

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Protoplasm culture following polyethylene glycol-induced fusion resulted in the regeneration of tetraploid somatic hybrid plants from the following attempted parental combinations: Cleopatra mandarin (Citrus reticulata Blanco) + Argentine trifoliate orange [Poncirus trifoliata (L.) Raf.]; `Succari' sweet orange [C. sinensis (L.) Osb.] + Argentine trifoliate orange; sour orange (C. aurantium L.) + Flying Dragon trifoliate orange (P. trifolita); sour orange + Rangpur (C. limonia Osb.); and Milam lemon (purported sexual hybrid of C. jambhiri Lush × C. sinensis) + Sun Chu Sha mandarin (C. reticulate Blanco). Protoplasm isolation, fusion, and culture were conducted according to previously published methods. Regenerated plants were classified according to leaf morphology, chromosome number, and peroxidase, phosphoglucomutase, and phosphoglucose isomerase leaf isozyme profiles. All of the somatic hybrid plants were tetraploid, as expected (2n = 4x = 36), and all five selections have been propagated and entered into commercial citrus rootstock trials.
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Girardi, Eduardo Augusto, Francisco de Assis Alves Mourão Filho, and Sônia Maria de Stefano Piedade. "Desenvolvimento vegetativo e custo de produção de porta-enxertos de citros em recipientes para fins de subenxertia." Pesquisa Agropecuária Brasileira 42, no. 5 (May 2007): 679–87. http://dx.doi.org/10.1590/s0100-204x2007000500010.

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O objetivo deste trabalho foi avaliar o desenvolvimento vegetativo e estimar o custo de produção de 11 porta-enxertos de citros para fins de subenxertia, em diferentes recipientes. Avaliaram-se limão 'Cravo' clone Limeira (Citrus limonia Osbeck); citrumelo 'Swingle' (Poncirus trifoliata (L.) Raf. x Citrus paradisi Macf.); tangerina 'Cleópatra' (Citrus reshni Hort. ex Tanaka); tangerina 'Sunki' (Citrus sunki Hort. ex Tanaka); limão 'Volkameriano' clone Catânia 2 (Citrus volkameriana Pasquale); laranja 'Caipira' clone DAC (Citrus sinensis L. Osbeck); limão 'Rugoso da África' clone Mazoe (Citrus jambhiri Lush.); Poncirus trifoliata 'Davis A'; tangerina 'Sun Shu Sha Kat' (Citrus sunki Hort. ex Tanaka); tangerina 'Sunki' clone 2506 ou Fruto Grande (Citrus sunki Hort. ex Tanaka) e Poncirus trifoliata 'Barnes'. Foram utilizados tubetes de 290 mL, sacolas de 1,7 L, e porta-enxertos transplantados de tubetes de 75 mL para sacolas de polietileno de 1,7 e 4,5 L. Porta-enxertos produzidos diretamente em sacolas de 1,7 L atingem ponto ideal de subenxertia em menor tempo, de 100 a 150 dias após a semeadura, e permitem a obtenção de plantas maiores e com sistema radicular adequado, porém com custo de produção superior ao sistema de produção em tubetes de 290 mL.
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Mendes-da-Glória, Fernanda Januzzi, Francisco de Assis Alves Mourão Filho, and Beatriz Appezzato-da-Glória. "Morfologia de embriões nucelares de laranja 'Valência' (Citrus sinensis (L.) Osbeck)." Acta Botanica Brasilica 15, no. 1 (April 2001): 17–25. http://dx.doi.org/10.1590/s0102-33062001000100003.

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Esse trabalho teve como objetivo avaliar a morfologia de embriões nucelares de laranja 'Valência' (Citrus sinensis (L.) Osbeck) a fim de fornecer informações para estudos de embriogênese somática induzida in vitro visando o melhoramento de citros que utilizem essa variedade. Para a realização dessas avaliações, embriões coletados de sementes provenientes de frutos maduros (13 meses após o florescimento) foram fixados, desidratados e preparados para a realização dos cortes histológicos seriados. Dos embriões avaliados, apenas um apresentou a morfologia de embrião zigótico. Os demais mostraram cotilédones com angulações e formatos distintos. Foram observados embriões mal formados com a não diferenciação de ápice caulinar e/ou ápice radicular, não desenvolvimento de um dos cotilédones, além de eixo embrionário colapsado. Os dados obtidos poderão auxiliar na interpretação dos tipos morfológicos de embriões somáticos obtidos in vitro e sua capacidade de conversão em plantas.
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Walter, Abigail J., YongPing Duan, and David G. Hall. "Titers of ‘Ca. Liberibacter asiaticus’ in Murraya paniculata and Murraya-reared Diaphorina citri Are Much Lower than in Citrus and Citrus-reared Psyllids." HortScience 47, no. 10 (October 2012): 1449–52. http://dx.doi.org/10.21273/hortsci.47.10.1449.

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Huanglongbing, one of the most devastating diseases of citrus, is associated with the bacterium ‘Candidatus Liberibacter asiaticus’ vectored by the Asian citrus psyllid, Diaphorina citri, in North America. Murraya paniculata is a common ornamental plant that is an alternate host of both the psyllid and bacterium. We tested M. paniculata and Citrus sinensis grown together in the same field for their titer of ‘Ca. L. asiaticus’. We found the bacterium in both M. paniculata and C. sinensis, but the titer was four orders of magnitude lower in M. paniculata. We also assayed D. citri from laboratory colonies reared on either ‘Ca. L. asiaticus’-infected M. paniculata or infected Citrus spp. Psyllids reared on infected M. paniculata also carried bacterial titers five orders of magnitude lower than psyllids reared on infected Citrus spp. These observations imply resistance to huanglongbing in M. paniculata.
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Castilho, Alison Pureza, Walkymário De Paulo Lemos, Pedro Carlos Strikis, Maria do Socorro Miranda Sousa, and Ricardo Adaime. "Species of Neosilba (Diptera: Lonchaeidae) recorded in citrus-producing region in the state of Pará, Brazil." Biotemas 30, no. 3 (September 5, 2017): 85. http://dx.doi.org/10.5007/2175-7925.2017v30n3p85.

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Este trabalho registra as espécies de Lonchaeidae obtidas de frutos de Citrus sinensis (L.) Osbeck coletados em áreas do polo citrícola do estado do Pará, Brasil. Três espécies de Lonchaeidae infestaram frutos de C. sinensis, sendo Neosilba glaberrima (Wiedemann) a predominante.
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Hansen, U., and G. Seufert. "Terpenoid emission from citrus sinensis (L.) OSBECK under drought stress." Physics and Chemistry of the Earth, Part B: Hydrology, Oceans and Atmosphere 24, no. 6 (January 1999): 681–87. http://dx.doi.org/10.1016/s1464-1909(99)00065-9.

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Ribeiro, Jose Pedro Nepomuceno, and Maria Inês Salgueiro Lima. "Allelopathic effects of orange (Citrus sinensis L.) peel essential oil." Acta Botanica Brasilica 26, no. 1 (March 2012): 256–59. http://dx.doi.org/10.1590/s0102-33062012000100025.

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Crop weeds are the main problem in agriculture, causing a worldwide annual loss of about US$95 billion. The principal method for control is the use of synthetic herbicides. The continued use of these products increases crop costs, reduces crop quality, and leaves toxic residues in the environment, which are a threat to human and livestock health. Therefore, there is a demand for environmentally friendly methods of weed control. The use of allelopathic compounds from crop residues is an alternative. Orange is one of the biggest crops in the world, and its cultivation generates large amounts of residues. There is strong evidence of bioactivity in orange peel essential oil. Therefore, the objective in this work was to verify the allelopathic proprieties of this oil. We extracted the oil from the peels of recently discarded oranges using water vapor flow with a Clevenger extractor, and tested it against the growth of Euphorbia heterophylla L. and Ipomoea grandifolia (Dammer) O'Donell seedlings when placed in contact with the oil vapor. The results were both quantitative and qualitative in the inhibition of the seedlings.
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RAI, P., CHHAYA RAI, G. C. MAJUMDAR, S. DASGUPTA, and S. DE. "STORAGE STUDY OF ULTRAFILTERED MOSAMBI (CITRUS SINENSIS(L.) OSBECK) JUICE." Journal of Food Processing and Preservation 32, no. 6 (December 2008): 923–34. http://dx.doi.org/10.1111/j.1745-4549.2008.00222.x.

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31

Jerković, Igor, Jasmina Družić, Zvonimir Marijanović, Mirko Gugić, Stela Jokić, and Marin Roje. "GC-FID/MS Profiling of Supercritical CO2 Extracts of Peels from Citrus aurantium, C. sinensis cv. Washington navel, C. sinensis cv. Tarocco and C. sinensis cv. Doppio Sanguigno from Dubrovnik Area (Croatia)." Natural Product Communications 10, no. 7 (July 2015): 1934578X1501000. http://dx.doi.org/10.1177/1934578x1501000745.

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The peels of Citrus aurantium L. and Citrus sinensis Osbeck cultivars from the Dubrovnik region (south Croatia) were extracted by supercritical CO2 at 40°C and 10 MPa at 1.76 kg/h to obtain enriched extracts in comparison with simple pressing of the peels. The extracts were analyzed in detail by gas chromatography and mass spectrometry (GC-FID/MS). Relevant similarities among the peel oil compositions of C. aurantium and C. sinensis cultivars were found with limonene predominance (up to 54.3%). The principal oxygenated monoterpenes were linalool (3.0%–5.9%), α-terpineol (0.7%–2.4%), linalyl acetate (0.0%–5.0%), geranyl acetate (0.0%-0.4%), ( Z)-citral (0.0%–1.8%) and ( E)-citral (0.0%–1.9%). Several sesquiterpenes were found with minor percentages. Coumarin derivatives were identified in all the samples among the relevant compounds. Isogeijerin dominated in the peels of C. sinensis cv. Tarocco (15.3%) and C. aurantium (11.2%). Scoparone ranged from 0.1% to 0.5% in all the samples. Bergapten (up to 1.4%), osthole (up to 1.1%) and 7-methoxy-8-(2-formylpropyl)coumarin (up to 1.1%) were found mostly in C. sinensis cv. Doppio Sanguigno. It was possible to indicate a few other differences among the extracts such as higher percentage of linalool, linalyl and geranyl acetates, as well as the abundance of sabinene and isogeijerin in C. aurantium or the occurrence of β-sinensal in C. sinensis cultivars.
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Chahal, Tanjeet Singh, P. P. S. Gill, and Adesh Kumar. "Propagation of citrus rootstock-carrizo citrange (Citrus sinensis Osb. X Poncirus trifoliate L. Raf.)." Agricultural Research Journal 55, no. 4 (2018): 706. http://dx.doi.org/10.5958/2395-146x.2018.00128.x.

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33

Martati, E., and P. P. Ciptadi. "Extraction of baby java citrus (Citrus sinensis (L) Osbeck) peel by microwave-assisted extraction." IOP Conference Series: Earth and Environmental Science 443 (March 26, 2020): 012020. http://dx.doi.org/10.1088/1755-1315/443/1/012020.

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34

Gonçalves, Ana Paula, Karina Silva dos Santos, Camila de Cassia Silva, Tanara Garcia de Novaes, and Rúbia de Oliveira Molina. "Identificação de isolados de Citrus tristeza virus (CTV) protetivos para Citrus sinensis (L.) Osbeck." Summa Phytopathologica 44, no. 1 (March 2018): 17–22. http://dx.doi.org/10.1590/0100-5405/172270.

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RESUMO O Citrus tristeza virus (CTV) causa significativas perdas na produtividade de laranja doce [Citrus sinensis (L.) Osbeck] e seu controle tem sido realizado principalmente com a premunização. O trabalho teve como objetivo analisar a variabilidade de isolados fortes e fracos de CTV provenientes de plantas de citros inoculadas e mantidas em casa de vegetação e amostras de campo, coletadas em pomar comercial situado no município de Rolândia, PR. Para a determinação da variabilidade e diversidade genética dos isolados foi realizada avaliação dos sintomas e empregadas as técnicas de RT– PCR e RFLP, utilizando os oligonucleotídeos específicos HCP1/HCP2 e posterior sequenciamento dos fragmentos amplificados. Na avaliação de canelura, os isolados mantidos em casa de vegetação induziram sintomas leves, com exceção do isolado severo Capão Bonito. Os sintomas mais severos ocorreram em amostras situadas no campo. De acordo com as análises multivariadas os isolados de CTV tendem a se agrupar conforme a severidade dos sintomas e condições ambientais as quais foram expostas formando agrupamentos distintos entre amostras provenientes do campo e casa de vegetação. O dendrograma gerado a partir do sequenciamento dos isolados e as análises multivariadas revelaram que o isolado proveniente da amostra “Forte Arapongas” apresentou maior similaridade com o controle padrão forte proveniente de Capão Bonito. Os isolados identificados como fracos e provenientes das amostras Pêra IAC e Rolândia 5 apresentaram maior similaridade. Pode-se aferir que plantas hospedeiras mantidas em campo possuem maior variabilidade de isolados.
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35

Rouse, Robert E., David P. H. Tucker, and Edgar D. Holcomb. "INJURY TO 27 CITRUS CULTIVARS ON 22 ROOTSTOCKS AT ONE-YEAR-OLD EXPERIENCING MINIMUM TEMPERATURE OF -4.4C." HortScience 25, no. 9 (September 1990): 1137b—1137. http://dx.doi.org/10.21273/hortsci.25.9.1137b.

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Evaluation ratings of cold injury following a freeze on December 24 & 25, 1989, showed differences among scion cultivars and rootstock. `Star Ruby' grapefruit (Citrus paradisi Macf.) and `Fallglo' citrus hybrid, a cross of Bower mandarin citrus hybrid × Temple tangor (C. temple Hort. ex Y. Tanaka) were the most severely damaged scion cultivars. `Rohde Red' valencia orange selection 472-11-43. [C. sinensis (L.) Osbeck]. was the least damaged scion cultivar. Scions budded to Cleopatra mandarin (C. reshni Hort. ex Tan.) and FL 80-18 citrumelo [C. paradisi × Poncirus trifoliata (L.) Raf.] rootstock were damaged more than on other rootstock. Scions budded to smooth flat seville (C. aurantium?) and P. trifoliata × Ridge pineapple sweet orange selection 1573-26 [C. sinensis (L.) Osbeck] had the least injury. Analysis comparing replications showed the greatest damage to be in the north side of the planting.
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36

Verdejo-Lucas, S., F. J. Sorribas, J. B. Forner, and A. Alcaide. "Screening Hybrid Citrus Rootstocks for Resistance to Tylenchulus semipenetrans Cobb." HortScience 32, no. 6 (October 1997): 1116–19. http://dx.doi.org/10.21273/hortsci.32.6.1116.

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The response of 22 citrus hybrid rootstocks to a Mediterranean biotype of Tylenchulus semipenetrans Cobb was determined in a greenhouse test. Five of the 11 rootstocks with Poncirus trifoliata (L.) Raf in their parentage did not support nematode reproduction. However, rootstocks with Troyer citrange [Citrus sinensis (L.) Osbeck × P. trifoliata (L.) Raf] in their parentage were susceptible to the nematode.
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37

Williamson, Jeffrey G., Karen E. Koch, and William S. Castle. "CITRUS ROOTSTOCK SHOOTS ENHANCE SCION GROWTH AFTER BUD FORCING." HortScience 25, no. 9 (September 1990): 1152d—1152. http://dx.doi.org/10.21273/hortsci.25.9.1152d.

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Carrizo citrange [Citrus sinensis (L.) Osb. × Poncirus trifoliata (L.) Raf.] seedlings were budded with `Hamlin' orange [Citrus sinensis (L.) Osb.] and subjected to 3 bud forcing treatments: (1) topping [T] by removing the seedling above the bud union; (2) lopping [L] by cutting half way through the seedling above the bud union and breaking the rootstock over; or, (3) bending [B] the seedling top over and tying it to the base of the plant. As scion buds emerged and grew, plants were sacrificed for dry weight measurements; also, the portion of the rootstock seedling above the bud union was exposed to 14CO2 at 3 stages of scion development. Plants with seedling tops attached (B,L) gained more dry weight and fibrous roots than T seedlings. Scion elongation was greater for B plants than for T plants. Plants usually flushed twice regardless of bud forcing treatment. No treatment differences were noted for time of flushing or scion bud emergence. Labeled photosynthate from attached rootstock leaves was translocated to scions during both flushes suggesting that recently fixed C enhanced scion growth for B and L plants.
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38

Toffano, Leonardo, Ivan Herman Fischer, Silvia Blumer, and Sérgio Florentino Pascholati. "Potencial do flavedo (epicarpo) de Citrus aurantifolia cv. Tahiti no controle do bolor verde e da antracnose em citros." Summa Phytopathologica 38, no. 1 (March 2012): 61–66. http://dx.doi.org/10.1590/s0100-54052012000100010.

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O Brasil é considerado o maior produtor de citros e o maior exportador de suco de laranja. Doenças de pós-colheita representam uma grande perda para a citricultura, sendo que para a exportação de frutos são rígidas as exigências com relação a isenção de resíduos químicos nos mesmos. Patógenos de importância em pós-colheita de citros incluem o Penicillium digitatum, agente causal do bolor-verde e o Colletotrichum gloeosporioides, agente causal da antracnose. Dada a importância econômica que representam estas doenças dos frutos cítricos, tanto em termos de comprometimento da qualidade e dificuldade de controle, a busca de alternativas adicionais que possam viabilizar a capacidade produtiva e garantir a obtenção de frutos com excelentes padrões de qualidade torna-se imprescindível. Portanto, estudou-se os efeitos dos extratos aquosos do flavedo de Citrus aurantifolia var. Tahiti, Lentinula edodes, Agaricus subrufescens (syn. Agaricus brasiliensis), albedo de Citrus sinensis var. Valência e do ácido jasmônico no controle póscolheita do bolor verde e da antracnose e na indução de resistência em frutos de laranjeira Valência (Citrus sinensis). Foi possível observar que o extrato aquoso do flavedo (C. aurantifolia) apresentou efeito inibitório sobre os patógenos, quando tratados em pós-colheita, em função da redução dos sintomas e esporulação. Porém, os extratos de albedo (C. sinensis), L. edodes, A. subrufescens e o ácido jasmônico não apresentaram efeitos sobre P. digitatum e C. gloeosporioides.
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39

Scholberg, J. M. S., L. R. Parsons, and T. A. Wheaton. "507 Nitrogen Uptake Dynamics of Citrus Seedlings." HortScience 35, no. 3 (June 2000): 482B—482. http://dx.doi.org/10.21273/hortsci.35.3.482b.

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Improving our understanding of processes that control and limit nitrogen uptake by citrus can provide a scientific basis for enhancing nitrogen fertilizer use efficiency. Nitrogen uptake dynamics of two rootstock seedlings will be compared to those of young budded trees. Three-month old Swingle citrumelo [Citrus paradisi Macf. × Poncirus trifoliata (L.) Raf.] and Volkamer lemon (C. volkameriana Ten. & Pasq.) trees were planted in PVC columns filled with a Candler fine sand. Field experiments were conducted using 4-year-old `Hamlin' orange trees [Citrus sinensis (L.) Osb.] grafted on `Carrizo' [C. sinensis × Poncirus trifoliata (L.) Raf.] or on Swingle citrumelo. Trees were either grown in solution culture using 120-L PVC containers or in 900-L PVC tubs filled with a Candler fine sand. Additional trees were planted in the field during Spring 1998. Two lateral roots per tree were trained to grow in slanted, partly burried, 20-L PVC columns filled with a Candler fine sand. Nitrogen uptake from the soil was determined by comparing the residual N extracted by intensive leaching from planted units with that of non-planted (reference) units. With the application of dilute N solutions (7 mg N/L), plants reduced N concentrations to near-zero N concentrations within days. Applying N at higher concentrations (70 or 210 mg N/L) resulted in higher initial uptake rates, increased residual soil N levels, and reduced nitrogen uptake efficiency. Contributions of passive uptake to total nitrogen uptake ranged from less than 5% at soil solution concentrations around 3 ppm N to 20% to 30% at concentrations of 60 ppm N.
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40

Tisserat, B., D. Jones, and P. Galletta. "Adventitious juice vesicle initiation in lemon (Citrus limon L.), mandarin (Citrus reticulata Blanco), sour orange (Citrus aurantium L.), and sweet orange (Citrus sinensis (L.) Osb.) fruit explants." Experientia 44, no. 8 (August 1988): 722–24. http://dx.doi.org/10.1007/bf01941044.

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41

Martínez-Pérez, D., L. C. Donadio, and O. R. Sempionato. "DESARROLLO Y PRODUCTIVIDAD DE CULTIVARES DE NARANJA DULCE (Citrus sinensis L. Osbeck) INJERTADAS EN CITRUMELO ‘SWINGLE’." Revista Chapingo Serie Horticultura IV, no. 02 (December 1998): 119–24. http://dx.doi.org/10.5154/r.rchsh.1998.04.039.

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42

Bastianel, Marinês, Mariângela Cristofani-Yaly, Antonio Carlos de Oliveira, Juliana Freitas-Astúa, Antonio Augusto Franco Garcia, Marcos Deon Vilela de Resende, Vandeclei Rodrigues, and Marcos Antônio Machado. "Quantitative trait loci analysis of citrus leprosis resistance in an interspecific backcross family of (Citrus reticulata Blanco × C. sinensis L. Osbeck) × C. sinensis L. Osb." Euphytica 169, no. 1 (May 8, 2009): 101–11. http://dx.doi.org/10.1007/s10681-009-9950-3.

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43

Trovato, A., M. T. Monforte, R. Barbera, A. Rossitto, E. M. Galati, and A. M. Forestieri. "Effects of fruit juices of Citrus sinensis L. and Citrus limon L. on experimental hypercholesterolemia in the rat." Phytomedicine 2, no. 3 (January 1996): 221–27. http://dx.doi.org/10.1016/s0944-7113(96)80046-8.

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44

Tignor, Milton E., Frederick S. Davies, and Wayne B. Sherman. "Freezing Tolerance and Growth Characteristics of USDA Intergeneric Citrus Hybrids US 119 and Selection 17-11." HortScience 33, no. 4 (July 1998): 744–48. http://dx.doi.org/10.21273/hortsci.33.4.744.

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Two USDA intergeneric, hybrid citrus scions, US 119 {[Citrus paradisi Mac. `Duncan' × Poncirus trifoliata (L.) Raf.] × C. sinensis Osb. `Succory'} and selection 17-11 {C. grandis US 145 × [Citrus paradisi Mac. `Duncan' × P. trifoliata (L.) Raf.]} on `Swingle' citrumelo (C. paradisi × P. trifoliata) rootstocks were examined for freeze hardiness traits (4 years) and general growth characteristics (2 years). Hardiness was compared with that of `Hamlin' orange [C. sinensis (L.) Osb.] and satsuma mandarin (C. unshiu Marc) from Fall 1993 to Spring 1997. As expected, US 119 and 17-11 were both hardier than `Hamlin' orange as determined by leaf disc electrolyte leakage (EL). Both showed freezing tolerance similar to that of satsuma mandarin, but 17-11 was significantly hardier than satsuma or US 119 at several times during the 4-year study. Trunk diameter and tree height were similar for US 119 and selection 17-11.
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Tignor, Milton E., Frederick S. Davies, and Wayne B. Sherman. "Freezing Tolerance of Citrus Hybrids USDA 17-11 and USDA 119 Compared to `Hamlin' Orange and Satsuma Mandarin." HortScience 30, no. 4 (July 1995): 795B—795. http://dx.doi.org/10.21273/hortsci.30.4.795b.

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Citrus hybrids USDA 17-11 [Citrus grandis L. × (C. paradisi Macf. `Duncan' × Poncirus trifoliata (L.) Raf. `Gotha Road')] and 119 [(C. paradisi Macf. `Duncan' × P. trifoliata (L.) Raf. `Gotha Road') × C. sinensis (L.) Osb. `Succory'], `Hamlin' orange [C. sinensis (L.) Osb.], and satsuma mandarin (C. unshiu Marc.) were planted March 1993 and 1994. Trees were irrigated and fertilized in an identical manner. In 1993, electrolyte leakage readings were taken monthly using 17-11, 119, and satsuma leaf discs. Leaf killing point (LKP) LT50 averaged from –8 to – 9C by mid-November for all selections. In 1994, leaf discs from 17-11, 119, and `Hamlin' orange were sampled weekly to determine LKP. USDA 119 had the lowest LKP and acclimated the fastest during the fall. By the end of November, there was no significant difference in LKP (–6.5C) between USDA 119 and 17-11, although both selections were significantly more freeze-tolerant than `Hamlin' orange (LKP–40C), which showed no significant decrease in LKP until the 6 weeks after the hybrid selections began acclimating. Citrus hybrids 17-11 and 119 can survive in freeze-susceptible areas that are marginal for other commercial citrus.
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46

Falcinelli, Beatrice, Franco Famiani, Andrea Paoletti, Sara D’Egidio, Fabio Stagnari, Angelica Galieni, and Paolo Benincasa. "Phenolic Compounds and Antioxidant Activity of Sprouts from Seeds of Citrus Species." Agriculture 10, no. 2 (January 30, 2020): 33. http://dx.doi.org/10.3390/agriculture10020033.

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Seeds from Citrus species represent a relevant by-product of the juice industry and a potential source of bioactive compounds such as phenols and other antioxidants. Sprouting could be an intriguing idea to enhance the content of these compounds, as explored for other fruittree species. In this experiment, the sprouting performance, the concentration of total phenols and phenolic acids, and the antioxidant activity of seeds and sprouts were evaluated for bitter orange (Citrus aurantium L. seedlings), blonde orange (Citrus sinensis (L.) Osbeck cv.Biondocomune), sweet orange (Citrus sinensis (L.) Osbeck seedlings), lemon (Citrus limon (L.) Osbeck cv.Femminello), and mandarin (Citrus reticulata Blanco cv.Tardivo di Ciaculli). The germination was high for all genotypes except for mandarin, but it took 4–8 weeks. Sprouts did not differ among genotypes for size and generally had hard consistency of cotyledons and a bitter taste. The concentrations of total phenols and phenolic acids of seeds and sprouts varied with the genotype, while the antioxidant activity was not statistically different among treatments. Sprouting increased both the concentration of phenolic compounds and antioxidant activity but no correlation was found between them, suggesting that other antioxidants, besides phenols, are present. Given the slow germination and the bitter taste, Citrus sprouts appear unsuitable for homemade production aimed at direct consumption, while they may have perspectives for extraction of food additives, cosmetics, and pharmaceutics.
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47

Oliveira, Roberto Pedroso de, and Elizete Beatriz Radmann. "Genetic similarity of citrus fresh fruit market cultivars." Revista Brasileira de Fruticultura 27, no. 2 (August 2005): 332–34. http://dx.doi.org/10.1590/s0100-29452005000200037.

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The objective of this work was to assess the genetic similarity of the following citrus fresh fruit market seedless cultivars: Lane Late, Navelate, Navelina and Salustiana sweet oranges (Citrus sinensis (L.) Osbeck), Clemenules and Marisol mandarins (C. reticulata Blanco) and Okitsu satsuma mandarin (C. unshiu Marcovitch), and the hybrids Nova [C. clementina x (C. paradisi x C. tangerina)] and Ortanique (tangor probably derived from C. sinensis (L.) Osbeck x C. reticulata Blanco), utilizing isoenzymatic markers. Electrophoresis analysis of proteins extracted from leaf tissues was utilized to detect polymorphisms at ten isoenzymatic systems. Out of 30 alleles, 16 were polymorphic. The Jaccard coefficient was utilized to estimate the genetic similarity between the cultivars and the unweigthed pair-group method using an arithmetic average (UPGMA) was used to obtain the phenogram (NTSYS 1.7). The cultivars showed high genetic similarity (>72.5%), and were classified in five main groups: sweet oranges, 'Clemenules' and 'Marisol' mandarins, 'Nova', 'Ortanique', and 'Okitsu' satsuma mandarin.
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48

Grosser, Jude W., Frederick G. Gmitter, Franca Sesto, Xiu Xin Deng, and J. L. Chandler. "Six New Somatic Citrus Hybrids and Their Potential for Cultivar Improvement." Journal of the American Society for Horticultural Science 117, no. 1 (January 1992): 169–73. http://dx.doi.org/10.21273/jashs.117.1.169.

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Protoplasm culture following polyethylene glycol (PEG) -induced fusion resulted in the regeneration of somatic hybrid plants from the following six parental combinations: Citrus sinermis (L.) Osbeck cv. Hamlin + Severinia buxifolia (Poir.) Tenore (Chinese box-orange); C. reticulate Blanco cv. Cleopatra + Poncirus trifoliata (L.) Raf. cv. Flying Dragon; C. reticulate cv. Cleopatra + Swingle citrumelo (C. paradisi Macf. × P. trifoliata); C. sinensis cv. Hamlin + C. jambhiri cv. Rough lemon; C. sinensis cv. Valencia + C. jambhiri cv. Rough lemon; and C. paradisi cv. Thompson + `Murcott' tangor (purported hybrid of C. reticulate × C. sinensis). Diploid plants were regenerated from nonfused embryogenic culture-derived protoplasts of `Cleopatra' mandarin and `Hamlin' and `Valencia' sweet orange, and from nonfused leaf-derived protoplasts of Rough lemon and `Mnrcott'. Regenerated plants were classified according to leaf morphology, chromosome number, and isozyme analyses. All of the somatic hybrids reported herein are tetraploid (2n = 4x = 36), with the exception of the `Hamlin' + S. buxifolia hybrid, which was unexpectedly found to have a chromosome number of 2n = 27. These six new somatic hybrids have potential in citrus scion and rootstock improvement for commercial use.
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49

Mueller, Tobias G., Hanna M. Kahl, Bodil N. Cass, Elizabeth E. Grafton-Cardwell, and Jay A. Rosenheim. "Differential Impacts of Citrus Thrips Across Sweet Orange and Mandarin Species." Journal of Economic Entomology 112, no. 6 (July 1, 2019): 2767–73. http://dx.doi.org/10.1093/jee/toz178.

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Abstract Several domesticated Citrus species are grown as major commercial crops in California. Despite this, farmers currently use a single set of management practices, originally created for sweet oranges (Citrus sinensis (L.) Osbeck [Sapindales: Rutaceae]), for both sweet oranges and all mandarin species. Mandarins, primarily Citrus reticulata Blanco, Citrus clementina hort. ex Tanaka, and Citrus unshiu Marcovitch, comprise almost 25% of California citrus acreage, and little work has been done to assess host–pest interactions for these species. Citrus thrips (Scirtothripscitri Moulton [Thysanoptera: Thripidae]) are one of the main pests in California citrus and are major targets for early spring, “petal fall” insecticide applications. We used mixed species citrus blocks to test the influence of Citrus species, including C. sinensis, C. reticulata, C. clementina, and C. unshiu, on 1) citrus thrips densities following petal fall; 2) citrus thrips-induced scarring on both the calyx and stylar ends of fruit; and 3) fruit deformation. Citrus sinensis and C. unshiu had relatively high citrus thrips densities and scarring levels, whereas C. reticulata had lower densities of citrus thrips and scarring levels. The age structure of citrus thrips populations also varied across Citrus species. Fruit deformity associated with citrus thrips scarring was found on all Citrus species examined. Scarring on the stylar-end of fruit, a previously largely ignored location of citrus thrips scarring, was found to be common in C. reticulata. It is clear from our work that species-specific management guidelines for citrus thrips are needed in sweet oranges and mandarins.
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50

Rapisarda, Paolo, Paolo Pannuzzo, Gabriella Romano, and Giuseppe Russo. "Juice Components of a New Pigmented Citrus HybridCitrus sinensis(L.) Osbeck ×Citrus clementinaHort. ex Tan." Journal of Agricultural and Food Chemistry 51, no. 6 (March 2003): 1611–16. http://dx.doi.org/10.1021/jf020871n.

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