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Dissertations / Theses on the topic 'Cleavage assay'

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1

Wu, Wing-kei Ricky. "Development of an in vitro assay for MMP cleavage /." View the Table of Contents & Abstract, 2005. http://sunzi.lib.hku.hk/hkuto/record/B31494183.

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2

Wu, Wing-kei Ricky, and 胡永基. "Development of an in vitro assay for MMP cleavage." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2005. http://hub.hku.hk/bib/B4501050X.

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3

Zhao, Hongwei. "A Proteomic Study of Plant Messenger RNA Cleavage and Polyadenylation Specificity Factors and the Establishment of an In Vitro Cleavage Assay System." Oxford, Ohio : Miami University, 2008. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=miami1218547019.

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4

Dampanaboina, Lavanya. "FUNCTIONAL CHARACTERIZATION OF WD REPEAT PROTEINS, AtCstF50 AND AtFY IN CLEAVAGE AND POLYADENYLATION." UKnowledge, 2011. http://uknowledge.uky.edu/pss_etds/2.

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Polyadenylation is an essential post-transcriptional modification resulting in a mature mRNA in eukaryotes. Three cis-elements the Far Upstream Element (FUE), Near Upstream Element (NUE), and Cleavage Site (CS) - guide the process of cleavage and polyadenylation with the help of multi-subunit protein complexes cleavage and polyadenylation specificity factor (CPSF), cleavage stimulation factor (CstF) along with cleavage factors and poly(A) polymerase. Protein-protein interactions play an important role in the cleavage and polyadenylation process. WD repeat proteins play an important role in pro
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5

Zheng, Jun. "The Establishment and characterization of a novel plant in vitro cleavage and polyadenylation assay system." Miami University / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=miami1281031132.

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6

Setiawan, Careza [Verfasser], and Moritz [Akademischer Betreuer] Rossner. "NRG1 cleavage assay and small molecule screen for modulators of NRG1 processing / Careza Setiawan ; Betreuer: Moritz Rossner." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2018. http://d-nb.info/1168145848/34.

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7

Vosyka, Oliver [Verfasser], Steven [Akademischer Betreuer] Verhelst, Aphrodite [Akademischer Betreuer] Kapurniotu, and Stefan [Akademischer Betreuer] Lichtenthaler. "Assay development for monitoring intramembrane (Rhomboid) serine protease substrate cleavage and inhibition / Oliver Vosyka. Gutachter: Aphrodite Kapurniotu ; Stefan Lichtenthaler ; Steven Verhelst. Betreuer: Steven Verhelst." München : Universitätsbibliothek der TU München, 2013. http://d-nb.info/1048176185/34.

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8

Gupte, Varsha [Verfasser], and Jürgen [Akademischer Betreuer] Wehland. "Development of a high throughput compatible cell assay based on the proteolytic cleavage or inhibition of the human La protein / Varsha Gupte ; Betreuer: Jürgen Wehland." Braunschweig : Technische Universität Braunschweig, 2009. http://d-nb.info/1175829234/34.

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9

TALASILA, PHANI KUMAR. "NOVEL THYROID HORMONE TARGET GENES IN THE LIVER, AND THEIR ROLES IN THYROID HORMONE SIGNALING AND PHYSIOLOGY." Kent State University / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=kent1347474067.

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10

Chang, Chien-Chih, and 張健治. "Establishment of a MALT1-Mediated Proteolytic Cleavage Assay in vitro." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/92103927100306870552.

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碩士<br>國立臺灣大學<br>微生物學研究所<br>98<br>MALT1 (mucosa-associated-lymphoid-tissue lymphoma-translocation gene 1) acts as an adaptor protein with proteolytic activity that controls T/B-cell activation by regulating key molecules in T/B-cell-receptor-induced signaling pathways. It plays a central role in MALT lymphoma by its interaction with BCL10 and enforced activation of NF-κB signaling. Computer assisted amino acid sequence analysis indicated that MALT1 shared sequence similarity with caspases. However, MALT1 was not able to cleave substrates of caspases. It was, therefore, defined as a “paracas
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11

Nie, Bei. "Genome-wide human SNP genotyping using the surface invasive cleavage assay." 2006. http://www.library.wisc.edu/databases/connect/dissertations.html.

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12

Phillips, Margaret F. "Towards a high-throughput surface invasive cleavage assay using the maskless array synthesizer." 2008. http://www.library.wisc.edu/databases/connect/dissertations.html.

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13

Schoonover, Michelle Lea. "Investigation of G-Quadruplex DNA cleavage through development of a solution-based fluorescent assay." Thesis, 2012. http://hdl.handle.net/2152/30922.

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In vitro, G-rich sequences form highly stable secondary structures known as G-Quadruplexes. These structures have been characterized by circular dichroism nuclear magnetic resonance and X-ray crystallography; although their detection in vivo has remained elusive. Due to the biological implication of a transisent and polymorphic secondary structure forming within the hypothetical G-Quadruplex forming regions, there is growing interest to understand their in vivo molecular dynamics.<br>text
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14

Li, Ziyi. "An Investigation of Autoxidation and DNA Thermal Cleavage by Polymethine Cyanine Dyes and Analogs in Aqueous Solutions." 2015. http://scholarworks.gsu.edu/chemistry_theses/79.

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Studies on a series of polymethine cyanine dyes and analogs (1-24) show that certain near-infrared cyanines are capable of damaging DNA in the absence of light and external reducing agents. Experimental results imply that in this DNA thermal cleavage, the cyanine reduces Cu(II) to Cu(I) which reacts with O2 to generate the reactive oxygen species (ROS) O2∙- and ∙OH. The formation of these ROS is also thought to be responsible for the irreversible bleaching of the dyes in aqueous solutions. A correlation between structural features and DNA thermal cleavage activity as well as dye bleaching is s
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15

Kubíková, Jana. "Štěpení substrátů isoformami savčího Diceru." Master's thesis, 2016. http://www.nusl.cz/ntk/nusl-345029.

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Host organisms evolved antiviral responses, which can recognize the viral infection and deal with it. One of the frequent signs of viral infection in a cell is appearance of double-stranded RNA (dsRNA). One of the pathways responding to dsRNA is RNA interference (RNAi), which functions as the key antiviral defence system in invertebrates and plants. Mammals, however, utilize for antiviral defence a different dsRNA-sensing pathway called the interferon response. RNAi functions only in mammalian oocytes and early embryonal stages although its enzymatic machinery is present in all somatic cells,
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16

Chang, Wen-Yu, and 張文郁. "The infectivity assays of papaya ringspot virus contained the mutated coat protein cleavage sites." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/78326853641944692467.

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碩士<br>大葉大學<br>分子生物科技學系碩士班<br>93<br>A polyprotein of Papaya ringspot virus (PRSV) is initially synthesized from the vial genome, followed a proteolytic process by three virus-encoded proteinase P1, HC-Pro, and NIa. According to NIa cleavage rule, there were two possible cleavage sites located between NIb and coat protein (CP), and each was 20 amino acid apart. The sequence of the upstream cleavage site is VYHE/SRGTD (named CP1 cut site) and the other cleavage site is VFHQ/SKNE (named CP2 cut site). The double cleavage sites present in PRSV were special in potyviruses. To investigate the pos
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17

Yu-ChiehHsiao and 蕭宇傑. "Cleavable Peptide Synthesis on Polydimethylsiloxane (PDMS)-based Microarrays and Its Application for PKA Kinase Assay." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/45885866078939904334.

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碩士<br>國立成功大學<br>化學系<br>102<br>Solid state synthesis of cleavable peptides on the surface of gold nanoparticle-coated poly(dimethylsiloxane) (PDMS) substrate was demonstrated as an array format using a 5-mer molecule of poly-Trp5 as an example and heterobifunctional crosslinkers as the anchoring layer, t-Boc chemistry/ trifluoroacetic acid (TFA) for protection and de-protection, and reducing agents for peptide cleavage. The method was judiciously designed to use reagents and solvents that are compatible with the PDMS polymer substrate and the formation of the covalent binding during the synthes
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18

Lin, Bi-Yun, and 林碧雲. "The infectivity assays of papaya ringspot virus contained the mutation at the dual coat protein cleavage sites." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/24802097226057344604.

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碩士<br>大葉大學<br>分子生物科技學系碩士班<br>98<br>The genome of Papaya ringspot virus ( PRSV) contains 10,326 nucleotides that encode a 381 kDa protein processed by three viral proteinase P1, HC-Pro, and NIa. NIa is responsible for the processing of at least six cleavage sites in the C-terminal part of the polyprotein. According to the cleavage rule of NIa, there were two consensus cleavage sequences located between NIb and coat protein (CP), each represented as CP1 and CP2 cut site, and as a results, two in-frame heterologous N-terminal CP would be produced. The double cleavage sites between NIb and CP
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